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isolation using triflic acid solvolysis and identification of n(epsilon)-[(r)-1-carboxyethyl]-n(alpha)-(d-galacturonoyl)-l-lysine as a component of the o-specific polysaccharide of proteus mirabilis o13.an amino acid was released from the o-specific polysaccharide of proteus mirabilis o13 by acid hydrolysis and identified as n(epsilon)-[(r)-1-carboxyethyl]-l-lysine by comparison with the authentic sample. an amide of this amino acid with d-galacturonic acid was isolated from the polysaccharide by solvolysis with anhydrous trifluoromethanesulfonic (triflic) acid and characterised by 1h and 13c nmr spectroscopy. these and published data enabled determination of the full structure of the repeating ...200011072853
extreme functional sensitivity to conservative amino acid changes on enzyme exteriors.mutagenesis studies and alignments of homologous sequences have demonstrated that protein function typically is compatible with a variety of amino-acid residues at most exterior non-active-site positions. these observations have led to the current view that functional constraints on sequence are minimal at these positions. here, it is shown that this inference assumes that the set of acceptable residues at each position is independent of the overall sequence context. two approaches are used to t ...200010966772
periplasmic localization of a groes homologue in escherichia coli transformed with groesx cloned from legionella-like endosymbionts in amoeba proteus.escherichia coli mc4100 transformed with a groe homologous operon cloned from x-bacteria accumulated large amounts of the gene product when cultured at 30 or 37 degrees c. heat shock for 10-30 min at 42 degrees c or ethanol (5%) shock for 2 h increased groesx levels to about twice that in e. coli grown at 30 degrees c. the subcellular localization of groesx in transformed e. coli was determined by several subcellular fractionation methods, by the analysis of extracted proteins in sds polyacrylam ...200011081576
structural and serological studies of the related o-specific polysaccharides of proteus vulgaris o21 and proteus mirabilis o48 having oligosaccharide-phosphate repeating units.the o-specific polysaccharide chains (o-antigens) of the lipopolysaccharides (lpss) of proteus mirabilis o48 and proteus vulgaris o21 were found to have tetrasaccharide and pentasaccharide repeating units, respectively, interlinked by a glycosidic phosphate. polysaccharides and an oligosaccharide were derived from the lpss by various degradation procedures and studied by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,13c and 1h,31p hmqc experiments. the following rel ...200011082201
the structure of the carbohydrate backbone of the core-lipid a region of the lipopolysaccharide from proteus vulgaris serotype o25.the following structure of the lipid a-core region of the lipopolysaccharide (lps) from proteus vulgaris serotype o25 was determined by using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of lps, of the products of alkaline deacylation of the lps, and of the products of lps deamination: [structure: see text] terminal residues of beta-glcnac and beta-kdo (indicated by bold italics) are present alternatively in approximately 3:2 amount, leaving no unsubsti ...200011093709
evaluation of a method using proteus mirabilis and pseudomonas aeruginosa to experimentally induce dual infection of the urinary bladder in dogs.to evaluate a method using proteus mirabilis and pseudomonas aeruginosa to experimentally induce dual infection of the urinary bladder in dogs.200011131585
human anti-scrub typhus rickettsia and rabbit anti-proteus antibodies recognize similar epitope in the o-polysaccharide part of proteus mirabilis oxk lipopolysaccharide.in the weil-felix test, sera from patients infected with orientia tsutsugamushi reacted with lipopolysaccharide (lps) from proteus mirabilis oxk strains. the o-polysaccharide of p. mirabilis oxk lps consisted of pentasaccharide repeating units, with amidically-linked lysine residues. the lysine, linked to galacturonic residues, which plays an important role in the reaction with rabbit anti-oxk antibodies, was revealed with the aid of synthetic antigens. using elisa, immunoglobulin m antibodies f ...200011131808
the binding of proteus mirabilis nonagglutinating fimbriae to ganglio-series asialoglycolipids and lactosyl ceramide.proteus mirabilis is a common opportunistic gram-negative uropathogen that infects the upper urinary tract. we have examined the role of the nonagglutinating fimbriae (naf) of p. mirabilis in mediating bacterial adhesion to cell surface receptors. purified naf of p. mirabilis were demonstrated to bind to a number of glycolipids, including asialo-gm1, asialo-gm2, and lactosyl ceramide (laccer) in solid-phase binding assays and in thin layer chromatography (tlc) overlay assays. furthermore, preinc ...200011068685
reduction of endogenous bacteria associated with catfish fillets using the grovac process.fresh catfish (ictalurus punctatus) fillets are known to be contaminated with a large number of spoilage and pathogenic bacteria. the grovac method, a new patented (u.s. 5,543,163) process, was evaluated for its efficacy in reducing the number of pathogens and spoilage microorganisms associated with food. this process involves using a processing solution containing ascorbic acid (aa) and sodium chloride (nacl), vacuum, and tumbling. a total of 51 bacterial isolates were isolated and identified f ...200010983798
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1998). i. susceptibility distribution].the frequencies of bacterial isolation and susceptibilities to antimicrobial agents were investigated on 538 bacterial strains isolated from patients with urinary tract infections (utis) in 9 hospitals during the period of june 1998 to may 1999. of the above bacterial isolates, gram-positive bacteria accounted for 30.3% and gram-negative bacteria accounted for 69.7%. susceptibilities of several isolated bacteria to antimicrobial agents were as follows; against enterococcus faecalis isolated from ...200010868300
characteristics of neonatal bacterial meningitis in a teaching hospital in taiwan from 1984-1997.during the period from 1984 to 1997, 85 bacterial meningitis neonates with positive cerebrospinal fluid cultures were treated. the ages of these patients ranged from 1 to 28 days. the male to female ratio was 1.7 to 1. the most common causative agent was group b beta-hemolytic streptococci (gbs, 31.8%), followed by escherichia coli (20%), proteus mirabilis (7.1%), enterobacter cloacae (5.9%), other streptococci excluding streptococcus pneumoniae (5.9%), chryseobacterium meningosepticum (5.9%), e ...200010917879
synthesis and antimicrobial activity of 4-carbethoxymethyl-2-[(alpha-haloacyl)amino] thiazoles and 5-nonsubstituted/substituted 2-[(4-carbethoxymethylthiazol-2-yl)imino]-4-thiazolidinones.4-carbethoxymethyl-2-[(chloroacetyl/alpha-chloropropionyl/al pha- bromobutyryl/alpha-chloro-(alpha-phenylacetyl)amino]thiazoles (i-iv) were synthesized by reacting 4-carbethoxymethyl-2-aminothiazole with chloroacetyl chloride, alpha-chloropropionyl chloride, alpha-bromobutyryl bromide and alpha-chloro-alpha-phenylacetyl chloride, respectively. furthermore, i-iv were refluxed with ammonium thiocyanate to give 2-[(4-carbethoxymethylthiazol-2-yl)imino]-4-thiazolidinones (v-viii). v was refluxed wit ...200010918953
sequence diversity of intervening sequences (ivss) in the 23s ribosomal rna in salmonella spp.intervening sequences (ivss) occur sporadically in the rrl (ribosomal rna large) genes for 23s ribosomal rna (rrna) at helix-25 (base pair 550) and helix 45 (base pair 1170) in several bacterial genera, including salmonella, yersinia, proteus, and providencia, representing the enterobacteriaceae, but are missing from other genera such as escherichia. these sequences are transcribed, but later excised without re-ligation during rnaseiii processing of the rrna, resulting in fragmented 23s rrna. th ...200010925202
multiple meningiomas, craniofacial hyperostosis and retinal abnormalities in proteus syndrome.because clinical evidence suggests that proteus syndrome may be caused by a somatic mutation during early development, resulting in mosaicism, the possible types of abnormalities and their clinical distributions are highly variable. here, we report on an unusual patient with proteus syndrome. manifestations included multiple meningiomas, polymicrogyria, and periventricular heterotopias. both eyes had epibulbar cystic lesions. the retina showed diffuse disorganization with nodular gliosis, retina ...200010925389
in vitro activity of 19 antimicrobial agents against 3513 nosocomial pathogens collected from 48 canadian medical centres. the canadian antimicrobial study group.antimicrobial resistance is a global concern. differentiation between susceptibility rates for nosocomial versus community pathogens is important epidemiologically because it impacts on the appropriate empirical selection of antimicrobial therapy for infected patients. we studied resistance rates for 3513 nosocomial pathogens from 48 canadian medical centres tested against 19 antimicrobial agents. the following are percent susceptibility for ceftazidime, ceftriaxone, ciprofloxacin, imipenem, net ...200010926444
mechanisms of action of rabbit cap18 on monolayers and liposomes made from endotoxins or phospholipids.we have investigated the mechanism of action of the cationic antimicrobial protein (18 kda) cap18 on liposomes and monolayers made from phospholipids and enterobacterial lipopolysaccharides (lps). cap18 intercalates into lipid matrices composed of lps from sensitive strains, weaker into those made of lps from a resistant strain (proteus mirabilis strain r45) or negatively charged phospholipids, but not into those composed of neutral phosphatidylcholine. from the combination of data obtained with ...200010931974
evaluation of the intestinal microflora in the rat model for esophageal adenocarcinoma.surgically induced duodenal reflux results in cancer development in the rat esophagus. one proposed mechanism of carcinogenesis relies on the production of carcinogens in the presence of bacterial overgrowth. against this background, intestinal microflora in the rat jejunum was analyzed before and after reflux-inducing surgery. total gastrectomy and esophagojejunostomy were performed on sprague-dawley rats to produce esophageal reflux of duodenal juice (n = 12). three days before surgery they we ...200011005330
inhibition of virulence factor expression and swarming differentiation in proteus mirabilis by p-nitrophenylglycerol.proteus mirabilis is a common cause of upper urinary tract infections that can involve invasion of host urothelial cells. the ability to invade urothelial cells is coupled closely to swarming, a form of multicellular behaviour in which vegetative bacteria differentiate into hyperflagellate, filamentous swarming cells capable of co-ordinated and rapid population migration. co-ordinate expression of virulence factors including urease, protease, haemolysin and flagellin during swarm-cell differenti ...200010933258
[renocolic fistula: a case report].a 78-year-old woman had disregarded pneumaturia since april 1998. in march 1999, computed tomography and barium enema were done to examine anemia and positive fecal occult blood, revealing a left renocolic fistula and bilateral renal stones. intravenous pyelography revealed a left staghorn calculus, non-functioning kidney, and right partial staghorn calculus. urinalysis showed pyuria and the culture grew proteus vulgaris and klebsiella oxytoca. smear and culture of the urine were negative for ac ...200010934611
[enterobacterial native valve endocarditis in the intensive care unit: report of two cases]. 200010909160
prevalence of multidrug resistant organisms in an intensive care burn unit.during january to december, 1998, analysis of an outbreak of infections in the burn intensive care unit (bicu) of the hospital attached to the dayanand medical college, ludhiana was carried out. a total of 868 clinical samples from 290 patients with more than 40 per cent thermal injury were investigated. these samples included 322 wound swabs, 325 blood and 221 urine samples. bacterial pathogens were isolated from 80, 62 and 48 per cent samples of pus, blood and urine respectively. among the nin ...200010935317
virulence of a proteus mirabilis atf isogenic mutant is not impaired in a mouse model of ascending urinary tract infection.proteus mirabilis, a common cause of urinary tract infection, produces a number of different fimbriae, including ambient temperature fimbriae (atf). these fimbriae are optimally expressed at 23 degrees c and their contribution to urinary tract infection has so far remained unknown. in the present study, a clinical isolate of p. mirabilis and an isogenic allelic replacement mutant unable to express atf were tested for their ability to cause infection in the ascending urinary tract infection model ...200011024353
crystallization and preliminary x-ray analysis of chondroitin sulfate abc lyases i and ii from proteus vulgaris.chondroitin sulfate abc lyases (e.c. 4.2.2.4) are broad-specificity glycosaminoglycan-degrading enzymes. their preferred substrates are chondroitin sulfate and dermatan sulfate, which are broken down to short oligosaccharides. proteus vulgaris produces two such lyases, abc lyase i and ii, with molecular weights of 112-113 kda. diffraction-quality crystals of both enzymes have been obtained by the hanging-drop vapour-diffusion method. abc lyase i crystallizes in space group p2(1)2(1)2(1), with un ...200010930840
endotoxins stimulate generation of superoxide radicals and lipid peroxidation in blood platelets.lipopolysaccharide (lps, endotoxin) is an important structural constituent of the membrane of gram-negative bacteria with a wide range of biological effects. it can activate blood platelets. the purpose of present study was to determine the direct effect of endotoxins from proteus mirabilis, differing significantly in their composition, on the generation of superoxide radicals and thiobarbituric acid reactive substances (tbars) in blood platelets. superoxide radicals were measured by means of su ...200011034442
effect of initial carbon sources on the performance of microbial fuel cells containing proteus vulgaris.mediator-coupled microbial fuel cells containing proteus vulgaris were constructed and the cell performance was tested. fuel cell efficiency depended on the carbon source in the initial medium of the microorganism. maltose and trehalose were not utilized substantially by p. vulgaris; however, their presence in the initial medium resulted in enhanced cell performance. in particular, galactose showed 63% coulombic efficiency in a biofuel cell after p. vulgaris was cultured in a trehalose-containin ...200010940867
a novel action of the proton pump inhibitor rabeprazole and its thioether derivative against the motility of helicobacter pylori.the motility of helicobacter pylori was maximum at 37 degrees c and at ph 6. a newly developed proton pump inhibitor, rabeprazole (rpz), and its thioether derivative (rpz-th) markedly inhibited the motility of h. pylori. the concentrations of the drug necessary to inhibit 50% of the motility were 0.25, 16, 16, and >64 microgram/ml for rpz-th, rpz, lansoprazole, and omeprazole, respectively. no such inhibitory effects were observed with h(2) blockers or anti-h. pylori agents. the motilities of ca ...200011036024
bacteriology of chronic otitis media in dar es salaam, tanzania.to determine the aetiology of chronic otitis media (com) in dar es salaam and to find out the shelf life of boric acid in spirit ear drops (baised).200010944833
cefdinir versus cefaclor in the treatment of uncomplicated urinary tract infection.this multicenter, double-blind, randomized, parallel-group study was conducted in europe, south africa, and australia to compare the clinical and microbiologic efficacy and the tolerability of a cephalosporin antibiotic, cefdinir, with those of cefaclor in the treatment of uncomplicated urinary tract infection.200010945508
ibc-1, a novel integron-associated class a beta-lactamase with extended-spectrum properties produced by an enterobacter cloacae clinical strain.a transferable beta-lactamase produced by a multidrug-resistant clinical isolate of enterobacter cloacae was studied. the bla gene was carried by a large (>80-kb) transmissible plasmid. nucleotide sequence analysis of cloned fragments revealed that it was part of a gene cassette carried by a class 1 integron along with other resistance genes, including aac(6')-ib. the encoded beta-lactamase, designated ibc-1, was a novel class a enzyme that hydrolyzed ceftazidime and cefotaxime and was inhibited ...200010952563
tem-72, a new extended-spectrum beta-lactamase detected in proteus mirabilis and morganella morganii in italy.a new natural tem-2 derivative, named tem-72, was identified in a proteus mirabilis strain and in a morganella morganii strain isolated in italy in 1999. compared to tem-1, tem-72 contains the following amino acid substitutions: q39k, m182t, g238s, and e240k. kinetic analysis showed that tem-72 exhibits an extended-spectrum activity, including activity against oxyimino-cephalosporins and aztreonam. expression of bla(tem-72) in escherichia coli was capable of decreasing the host susceptibility to ...200010952610
complement activation by proteus mirabilis negatively charged lipopolysaccharides.proteus mirabilis strains are human pathogens responsible for urinary tract infections and bacteremias and may be involved in rheumatoid arthritis. lipopolysaccharide (lps, bacterial endotoxin), the major component of the cell wall, is one of the virulence factors of proteus. in the presented studies, we have investigated complement activation by lpss isolated from p. mirabilis o10, o23, o30, and o43 strains, which differ in the number of negative coo- groups on their polysaccharide components. ...200011052177
[pyonephrosis: diagnosis and treatment: report of 14 cases].prior to the introduction of antibiotics, the treatment of pyelonephrosis frequently consisted of nephrectomy to remove the non-functional kidney, which was a potentially dangerous source of systemic infection. this approach was later modified as a result of the advances made in antibiotic therapy, and included vigorous antibiotic treatment and prompt drainage of the kidney. at present, percutaneous nephrostomy provides a means of draining off the pus and determining a possible residual renal fu ...200010953791
[tartrate-sensitive and tartrate-resistant acid phosphatases in amoeba proteus].in free-living amoeba proteus (strain b), acid phosphatase (acp) was examined by disc-electrophoresis in polyacrylamide gel. the tartrate-sensitive amebian acp was greatly inhibited by dithiothreitol and cu2+, and only partly inhibited by sodium orthovanadate, ammonium molybdate, edta, disodium salt and mg2+, ca2+, zn2+ and mn2+. on the contrary, it appeared to be resistant to sulfhydryl reagents--4(hydroxymercury) benzoic acid, sodium salt and n-ethylmaleimide. unlike the tartrate-sensitive enz ...200010953868
antimicrobial-resistance trends in bacterial isolates from companion-animal community practice in the uk.we conducted a longitudinal, retrospective investigation of antimicrobial resistance in bacterial isolates obtained from canine and feline clinical cases in veterinary community practice in uk (1989-1997). individual-drug resistance was examined using isolates of escherichia coli and staphylococcus spp. as gram-negative and gram-positive indicator organisms, respectively. the annual prevalence of resistance was calculated for each organism to each of nine (for e. coli) and 11 (for staphylococcus ...200010960713
[immunosenescence: delayed cutaneous hypersensitivity tests in independently-living chilean elderly individuals].delayed cutaneous hypersensitivity tests are a globally accepted test to assess cellular immunity in vivo. the quality and quantity of the response to these type of tests, varies in different populations.200010962854
genetic characterization of dna region containing the trh and ure genes of vibrio parahaemolyticus.we have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. here, we cloned and sequenced the 15,754-bp dna region containing the trh gene and the gene cluster for urease production from the chromosome of clinical v. parahaemolyticus (th3996). we found 16 open reading frames (o ...200010992480
in-vitro antimicrobial activity of cefpirome: a new fourth-generation cephalosporin against clinically significant bacteria.to study the in-vitro antimicrobial activity of cefpirome: a new fourth generation cephalosporin in comparison with other agents against clinically significant gram-negative and gram-positive bacteria.200010992706
capillary electrophoretic analysis of wild type and mutant proteus penneri outer membrane proteins.in this study the virulence factors, outer membrane proteins (omp), lipopolysaccharides (lps), hemolysin, and the in vivo and in vitro virulence of wild-type proteus penneri 357 and its two isogenic mutant variants--a transposon and a spontaneous mutant--were examined. the omps of these variants were analyzed by a new and fast technique, "dynamic sieving" capillary electrophoresis (ce). the omp profiles were dominated by two peaks (39 and 43 kda). in the p. penneri clone examined, both the trans ...200011001319
[psoas abscess: difficult initial clinical impression]. 200010974773
production of a tem-24 plasmid-mediated extended-spectrum beta-lactamase by a clinical isolate of pseudomonas aeruginosa.several pseudomonas aeruginosa strains, including one urinary isolate producing an extended-spectrum beta-lactamase tem-24, were isolated from a long-term-hospitalized woman. three tem-24-producing enterobacterial species (enterobacter aerogenes, escherichia coli, and proteus mirabilis) were isolated from the same patient. tem-24 and the resistance markers for aminoglycosides, chloramphenicol, and sulfonamide were encoded by a 180-kb plasmid transferred by conjugation into e. coli hb101.200010602754
introduction of a mini-gene encoding a five-amino acid peptide confers erythromycin resistance on bacillus subtilis and provides temporary erythromycin protection in proteus mirabilis.a 15-bp mini-gene was introduced into bacillus subtilis and into stable protoplast-like l-forms of proteus mirabilis. this mini-gene encoded the peptide mvlfv and modeled a fragment of escherichia coli 23s rrna responsible for e. coli erythromycin (ery) resistance. expression of the introduced mini-gene conferred permanent ery resistance on b. subtilis. in l-forms of p. mirabilis, the ery-protective effect was maintained in the course of several generations. herewith, the mechanism of ery resist ...200010620668
synthesis of cefminox by cell-free extracts of streptomyces clavuligerus.in vitro synthesis of cefminox by cell-free extracts of streptomyces clavuligerus was investigated using alpha-ketoglutarate, l-ascorbic acid, feso(4), s-adenosyl-l-methionine, and 7alpha-demethoxycefminox as the substrates. the formation of cefminox was detected both by a biological assay with proteus vulgaris gn 76/c-1 and by high performance liquid chromatography. although the conversion rate of 7alpha-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the poten ...200010620685
problems related to determination of mics of oximino-type expanded-spectrum cephems for proteus vulgaris.during in vitro susceptibility testing of clinical isolates of proteus vulgaris, we noted that the mics of several expanded-spectrum cephems were much higher in the broth microdilution method than in the agar dilution method (termed the mic gap phenomenon). here we investigated the mechanism of the mic gap phenomenon. cephems with the mic gap phenomenon were of the oximino type, such as cefotaxime, cefteram, and cefpodoxime, which serve as good substrates for inducible class a beta-lactamase (cu ...200010655366
immediate and long-term galvanotactic responses of amoeba proteus to dc electric fields.the long-term and immediate galvanotactic responses of amoeba proteus to the direct current electric fields (dcefs) were studied with the methods of computer-aided image analysis. it was found that in contrast to earlier reports, amoebae continued locomotion towards cathode (the negative pole) for hours and the increase in the field strength in the range 300-600 mv/mm caused the straightening of cell trajectories accompanied by the decreased frequency of the lateral pseudopods formation and less ...200010618163
dynamic aspects of the structured cell population in a swarming colony of proteus mirabilis.proteus mirabilis forms a concentric-ring colony by undergoing periodic swarming. a colony in the process of such synchronized expansion was examined for its internal population structure. in alternating phases, i.e., swarming (active migration) and consolidation (growth without colony perimeter expansion), phase-specific distribution of cells differing in length, in situ mobility, and migration ability on an agar medium were recognized. in the consolidation phase, the distribution of mobile cel ...200010629184
role and mechanism of action of c. pvuii, a regulatory protein conserved among restriction-modification systems.the pvuii restriction-modification system is a type ii system, which means that its restriction endonuclease and modification methyltransferase are independently active proteins. the pvuii system is carried on a plasmid, and its movement into a new host cell is expected to be followed initially by expression of the methyltransferase gene alone so that the new host's dna is protected before endonuclease activity appears. previous studies have identified a regulatory gene (pvuiic) between the dive ...200010629196
biological activities of lipopolysaccharides of proteus spp. and their interactions with polymyxin b and an 18-kda cationic antimicrobial protein (cap18)-derived peptide.the saccharide constituents of lipopolysaccharides (lps) of proteus spp. vary with the strain and contain unique components about which little is known. the biological activities of lps and lipid a from s- and r-forms of 10 proteus strains were examined. lps from all s-form proteus strains was lethal to d-(+)-galactosamine (galn)-loaded, lps-responsive, c3h/hen mice, but not to lps-hypo-responsive c3h/hej mice. p. vulgaris 025 lps evoked strong anaphylactoid reactions in n-acetylmuramyl-l-alanyl ...200010670563
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 63 from a new serogroup o68.lipopolysaccharide of proteus penneri strain 63 was degraded by mild acid to give a high molecular mass o-specific polysaccharide that was isolated by gel-permeation chromatography. sugar and methylation analyses and nmr spectroscopic studies, including two-dimensional 1h, 1h cosy, tocsy rotating-frame noe spectroscopy, h-detected 1h,13c and 1h,31p heteronuclear multiple-quantum coherence (hmqc), and 1h, 13c hmqc-tocsy experiments, demonstrated the following structure of the polysaccharide: wher ...200010632731
rapid turnover of flhd and flhc, the flagellar regulon transcriptional activator proteins, during proteus swarming.the enterobacterial flhdc master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. during proteus mirabilis differentiation into elongated hyperflagellated swarm cells, flhdc transcription is strongly but transiently increased. we show that concentration of the flhd and flhc proteins is also tightly controlled at the posttranslational level. this is achieved by protein degradation, which is most severe after differentiation when the half-lif ...200010633123
aortic ring abscess and aortoatrial fistula complicating fulminant prosthetic valve endocarditis due to proteus mirabilis. 200010625192
insertional transposon mutagenesis by electroporation of released tn5 transposition complexes.dna transposition is an important biological phenomenon that mediates genome rearrangements, inheritance of antibiotic resistance determinants, and integration of retroviral dna. transposition has also become a powerful tool in genetic analysis, with applications in creating insertional knockout mutations, generating gene-operon fusions to reporter functions, providing physical or genetic landmarks for the cloning of adjacent dnas, and locating primer binding sites for dna sequence analysis. dna ...200010625401
how do intestinal t cells sense the dietary and microbial environment? 200010648476
biosynthetically diverse compounds from a saltwater culture of sponge-derived aspergillus niger.the new compound, asperic acid (1), and the known compounds hexylitaconic acid (2), malformin c (3), pyrophen (4), and asperazine (5) were isolated from the saltwater culture of aspergillus niger derived from a caribbean sponge, hyrtios proteus. the structure elucidation of asperic acid is presented.200010650076
structure of the o-specific polysaccharide of proteus penneri 71 and classification of cross-reactive p. penneri strains to a new proposed serogroup o64.a neutral o-specific polysaccharide (o-antigen) was isolated from the lipopolysaccharide (lps) of the bacterium proteus penneri 71. on the basis of sugar analysis and 1h- and 13c-nmr spectroscopic studies, including two-dimensional cosy, 13c,1h heteronuclear cosy and roesy, the following structure of the trisaccharide repeating unit of the polysaccharide was established: -->3)-beta-d-glcpnac-(1-->4)-beta-d-glcpnac-(1-->3)-alpha-d-galp-(1-- > the polysaccharide has the same carbohydrate backbone ...200010651818
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 8 from a new serogroup o67.an acidic o-specific polysaccharide was obtained by mild acid degradation of the proteus penneri 8 lipopolysaccharide and found to contain d-glucose, d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, 2-acetamido-2,6-dideoxy-l-galactose (l-fucnac) and 2-aminoethyl phosphate (petn) in the ratios 2 : 1 : 1 : 1 : 1 : 1. 1h and 13c nmr spectroscopy was applied to the intact and dephosphorylated polysaccharides, and the following structure of the hexasaccharide repea ...200010651819
fragmentation of 23s rrna in strains of proteus and providencia results from intervening sequences in the rrn (rrna) genes.intervening sequences (ivss) were originally identified in the rrl genes for 23s rrna (rrl genes, for large ribosomal subunit, part of rrn operon encoding rrna) of salmonella enterica serovars typhimurium lt2 and arizonae. these sequences are transcribed but later removed during rnase iii processing of the rrna, resulting in fragmentation of the 23s species; ivss are uncommon, but have been reported in at least 10 bacterial genera. through pcr amplification of ivs-containing regions of the rrl g ...200010648538
functional morphology of the inner ear and underwater audiograms of proteus anguinus (amphibia, urodela).octavolateral sensory organs (auditory and lateral line organs) of cave salamander proteus anguinus are highly differentiated. in the saccular macula of the inner ear the complex pattern of hair cell orientation and the large otoconial mass enable particle displacement direction detection. additionally, the same organ, through air cavities within the body, enables detection of underwater sound pressure changes thus acting as a hearing organ. the cavities in the lungs and mouth of proteus are a r ...200010653179
the ultrastructure of photoreceptor cells in the pineal organ of the blind cave salamander, proteus anguinus (amphibia, urodela).we studied ultrastructure of the photoreceptor cells in the pineal organ of blind, depigmented, neotenic cave salamander, proteus anguinus. unlike in epigean vertebrates the outer segments of most photoreceptor cells consists of concentrically arranged lamellae, however; in few cells, the outer segments contain 7-9 plasma membrane disks. in both types of photoreceptor cells the outer segments enclose lumps of vesicles of different sizes. the photoreceptor cells of proteus anguinus are similar to ...200010653183
antibacterial activity of some folklore medicinal plants used by tribals in western ghats of india.a series of 30 indian folklore medicinal plants used by tribal healers to treat infections, were screened for antibacterial properties at 10 mg/ml concentration by using disc diffusion method against bacillus subtilis, escherichia coli, klebsiella aerogenes, proteus vulgaris, pseudomonas aerogenes and staphylococcus aureus. twenty plant species showed activity against one or more species of bacteria used in this assay; among them the leaf extracts of cassia occidentalis and cassia auriculata exh ...200010661885
microbiologic survey of long-term care facilities.we undertook a microbiologic survey of long-term care facilities to categorize bacteria found in cultures of residents. culture and sensitivity data were collected on 566 samples from indwelling bladder catheters, percutaneous gastrostomy tubes, nares, stool, wounds, pressure ulcers, and tracheostomies in 25 nebraska and iowa facilities. information was also collected on resident factors (eg, presence of indwelling urinary catheter, prior antibiotic administration) and institutional variables (e ...200010679131
biochemical sequence analyses of ges-1, a novel class a extended-spectrum beta-lactamase, and the class 1 integron in52 from klebsiella pneumoniae.klebsiella pneumoniae ori-1 was isolated in 1998 in france from a rectal swab of a 1-month-old girl who was previously hospitalized in cayenne hospital, cayenne, french guiana. this strain harbored a ca. 140-kb nontransferable plasmid, ptk1, that conferred an extended-spectrum cephalosporin resistance profile antagonized by the addition of clavulanic acid, tazobactam, or imipenem. the gene for ges-1 (guiana extended-spectrum beta-lactamase) was cloned, and its protein was expressed in escherichi ...200010681329
proteus syndrome and immunodeficiency.a 10 year old boy with proteus syndrome presented with a pericardial effusion of unknown aetiology. immunological investigation revealed low serum igg and iga, accompanied by low levels of specific antibodies to pneumococcal and haemophilus type b polysaccharides. circulating lymphocyte surface marker profile revealed t and b cell lymphopenia. this is the first report of hypogammaglobulinaemia occurring in the proteus syndrome.200010685928
structure of a glycerol teichoic acid-like o-specific polysaccharide of proteus vulgaris o12.a phosphorylated o-specific polysaccharide (o-antigen) was obtained by mild acid degradation of proteus vulgaris o12 lipopolysaccharide and studied by sugar and methylation analyses, 1h-, 13c- and 31p-nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, h-detected 1h, 13c and 1h, 31p heteronuclear multiple-quantum coherence experiments. it was found that the polysaccharide consists of pentasaccharide repeating units connected via a glycerol phosphate group, and has the following struc ...200010651815
occurrence and antibiotic sensitivity of enterobacteriaceae isolated from a group of jordanian patients with community acquired urinary tract infections.the type and antibiotic sensitivity of urinary tract pathogens may differ in various communities. of 207 isolates recovered from midstream urine specimens collected from a group of patients with community acquired urinary tract infections (uti), 86% were species of enterobacteriaceae. the most frequently recovered pathogens were escherichia coli (82%), klebsiella spp. (7.3%), proteus spp. (6.2%), enterobacter spp. (3.4%) and citrobacter spp. (1.1%). high rates of resistance were found against am ...200010697742
trends in the susceptibilities of proteus mirabilis isolates to quinolones. 200010702570
[the resistance of recent clinical isolates against isepamycin, other aminoglycosides and injectable beta-lactams].clinical isolates collected from clinical facilities across japan in 1998 were tested against five aminoglycosides and three beta-lactams. the resistance of 50 strains each of methicillin sensitive staphylococcus aureus, methicillin resistant staphylococcus aureus (mrsa), staphylococcus epidermidis, escherichia coli, citrobacter freundii, klebsiella pneumoniae, enterobacter sp., serratia sp., pseudomonas aeruginosa and proteus sp. (p. mirabilis 25 strains and p. vulgaris 25 strains) to the amino ...200010709145
proteus, a technical and management model for aquatic risk assessment of industrial spills.the assessment of risks to the aquatic environment related to industrial installations is a priority in environmental pollution control in the netherlands. major accidents to the surface water such as the sandoz incident, but also the high number of smaller accidents that occur every year has invoked the need for an effective method to assess these risks. two different models have been used in this field in the netherlands over several years. these two software applications, veris and risam were ...200010677674
evaluation of the discriminatory powers of the dienes test and ribotyping as typing methods for proteus mirabilis.a total of 63 clinical isolates of proteus mirabilis collected over a 19-month period were typed by the dienes test and ribotyping. ribotyping was performed using the fully automated riboprinter microbial characterization system (qualicon, wilmington, del.). isolates that were indistinguishable by the dienes test and/or ribotyping were characterized further by pulsed-field gel electrophoresis (pfge). most of the isolates represented unique strains as judged by the dienes test and ribotyping. for ...200010699000
trends of antimicrobial resistance in bacterial isolates from a small animal referral hospital.a longitudinal, retrospective investigation was made of antimicrobial resistance in bacterial isolates obtained from clinical cases in a small animal hospital between 1989 and 1997. isolates of escherichia coli and staphylococcus species were used as gram-negative and gram-positive indicator organisms, respectively, and the annual prevalence of antimicrobial resistance was calculated for each organism to each of nine (for e coli) and 11 (for staphylococcus species) appropriate antimicrobials, in ...200010706308
h-ns is a repressor of the proteus mirabilis urease transcriptional activator gene urer.expression of proteus mirabilis urease is governed by urer, an arac-like positive transcriptional activator. a poly(a) tract nucleotide sequence, consisting of a(6)ta(2)ca(2)tggta(5)ga(6)tga(5), is located 16 bp upstream of the sigma(70)-like urer promoter p2. since poly(a) tracts of dna serve as binding sites for the gene repressor histone-like nucleoid structuring protein (h-ns), we measured beta-galactosidase activity of wild-type escherichia coli mc4100 (h-ns(+)) and its isogenic derivative ...200010762273
mixed infection in adult bacterial meningitis.12 adult patients suffering from bacterial meningitis caused by mixed infection were identified at kaohsiung chang gung memorial hospital over a period of 13 years (1986-1998), and they accounted for 6.5% (12/184) of our culture-proven adult bacterial meningitis. the 12 cases included seven males and five females, aged 17-74 years. six of the 12 cases had community-acquired infections and the other six had nosocomially-acquired infections. ten of the 12 cases had associated underlying diseases, ...200010697784
structure of the o-specific polysaccharide of the bacterium proteus mirabilis o29.an o-specific polysaccharide was obtained by mild acid degradation of p. mirabilis o29 lipopolysaccharide (lps) and found to contain 2-acetamido-2-deoxy-d-galactose and d-glucuronic acid (d-glca) in the ratio 3:1. studies of the polysaccharide by 1h- and 13c-nmr spectroscopy including two-dimensional correlation spectroscopy (cosy), total correlation spectroscopy (tocsy), nuclear overhauser effect spectroscopy (noesy), and h-detected 1h,13c-heteronuclear multiple-quantum coherence (hmqc) experim ...200010713543
ctx-m-type beta-lactamases: an emerging group of extended-spectrum enzymes.ctx-m-type beta-lactamases constitute a novel group of class a beta-lactamases with extended-spectrum properties. they are encoded by transferable plasmids and found in various enterobacteria, mostly salmonella typhimurium, escherichia coli, klebsiella pneumoniae and proteus mirabilis. ctx-m enzymes share extensive sequence similarity with the chromosomal beta-lactamases of klebsiella oxytoca. they efficiently hydrolyze many newer broad-spectrum oximino-beta-lactams including cefotaxime, ceftria ...200010720804
ciprofloxacin in the treatment of chronic bacterial prostatitis: a prospective, non-comparative multicentre clinical trial with long-term follow-up. the german prostatitis study group.the efficacy and safety of ciprofloxacin 500 mg bd for 28 days were assessed in 65 adult males with symptomatic bacterial prostatitis, from eight centres in germany. urine and prostatic secretions were obtained for culture. clinical signs and symptoms were evaluated at 12-18 days during treatment, end of treatment (4-9 days post-treatment), and 1, 3, 6 and 9 months post-treatment. safety was monitored during and at the end of treatment. e. coli was the most frequent pathogen causing infection (3 ...200010720805
nucleotide sequence of the bla(rtg-2) (carb-5) gene and phylogeny of a new group of carbenicillinases.we determined the nucleotide sequence of the bla gene for the acinetobacter calcoaceticus beta-lactamase previously described as carb-5. alignment of the deduced amino acid sequence with those of known beta-lactamases revealed that carb-5 possesses an rtg triad in box vii, as described for the proteus mirabilis gn79 enzyme, instead of the rsg consensus characteristic of the other carbenicillinases. phylogenetic studies showed that these rtg enzymes constitute a new, separate group, possibly ance ...200010722515
microbial populations and volatile compounds in the 'bone taint' spoilage of dry cured ham.'bone taint' is one of the most important causes of spoilage of dry cured ham. this alteration is characterized by a foul-smelling odour. the microbial population and volatile compounds associated with incipient 'bone taint' were evaluated. enterobacteriaceae species were found at levels of 7.94 x 10(5) cfu g(-1) in spoiled hams and were not detected in unspoiled hams. serratia sp. and proteus sp. are the main organisms characterized. the volatile compounds from the spoiled hams give rise to hig ...200010728563
comparable endotoxic properties of lipopolysaccharides are manifest in diverse clinical isolates of gram-negative bacteria.in general there is a poor correlation between serum lipopolysaccharide (lps; the biologically active constituent of endotoxin) levels and mortality in septic patients. the objective of this study was to determine if chemical, structural, or biological differences among lps from different clinical isolates of gram-negative bacteria might explain this discrepancy. lps preparations were made using the hot phenol-water extraction method from eight clinical isolates of gram-negative bacteria. as a p ...200010722580
treatment of gram-negative bacterial meningitis in term neonates with third generation cephalosporins plus amikacin.the aim of this retrospective study was to evaluate the clinical efficacy in terms of mortality and long-term morbidity of third generation cephalosporins and amikacin in combination for the treatment of gram-negative bacterial meningitis in a homogeneous group of neonates. a 15-year experience (1983-1997) with 72 term neonates without central nervous system anomalies and with gram-negative organisms grown in their cerebrospinal fluid treated with the above combination of antibiotics is presente ...200010729716
minimum inhibitory concentration determinations for various antimicrobial agents against 1570 bacterial isolates from turkey poults.minimum inhibitory concentrations (mics) were determined for 1570 bacteria from eight geographic locations (1204 escherichia coli, 231 other enteric gram-negative bacilli [including citrobacter spp., enterobacter spp., klebsiella spp., proteus spp., and salmonella spp.], 31 pseudomonas spp., 18 coagulase-positive staphylococci, 26 coagulase-negative staphylococci, and 55 streptococci and enterococci) by the national committee for clinical laboratory standards broth microdilution procedure. antim ...200010737648
three-year surveillance study of nosocomial bacterial resistance in argentina. the antimicrobial committee; and the national surveillance program (sir) participants group.a national surveillance program (sir) was introduced in 1996 in argentina by the antimicrobial committee of the argentinean society for microbiology to assess bacterial resistance. the present study reports the rates of nosocomial bacterial resistance found by this program.200010737844
proteus syndrome: mri characteristics of plantar cerebriform hyperplasia.proteus syndrome is a rare congenital hamartomatous syndrome with a variety of abnormalities. it shares many features with other congenital hamartomatous disorders, but cerebriform hyperplasia of the soles and the palms is known as a quite distinctive characteristic in the dermatologic literature. the purpose of this case report is to demonstrate the mri features of plantar cerebriform hyperplasia in a 9-year-old boy with known proteus syndrome.200010741500
antimicrobial activity and spectrum of the new glycylcycline, gar-936 tested against 1,203 recent clinical bacterial isolates.the in vitro activity of gar-936, a new semisynthetic glycylcycline, was evaluated in comparison with two tetracyclines and several other antimicrobial agents. a total of 1,203 recent clinical isolates were tested by reference broth or agar dilution methods. among the members of the family enterobacteriaceae, gar-936 was generally two- to four-fold more active than minocycline, and two- to 16-fold more active than tetracycline. all enteric bacilli mic90 results were < or = 4 microg/ml; the excep ...200010744364
frequency and time course of pancreatic and extrapancreatic bacterial infection in experimental acute pancreatitis in rats.infectious complications in severe pancreatitis are the main factors determining clinical course and outcome. the taurocholate model for acute necrotizing pancreatitis was evaluated for frequency and time course of pancreatic and extrapancreatic bacterial infection.200010776434
is rheumatoid arthritis a form of reactive arthritis? 200010743787
infection and rheumatoid arthritis: guilt by association? 200010743788
antibiotic susceptibility of bacterial strains isolated from patients with community-acquired urinary tract infections in france. multicentre study group.the aim of this study was to determine the distribution and antibiotic susceptibility patterns of bacterial strains isolated from adults with community-acquired urinary tract infections (uti) in france. from december 1996 to march 1997, each of 15 private laboratories in france consecutively collected about 80 non-duplicate strains isolated from adult outpatients with uti, including patients receiving care at home, and tested their susceptibility by the disk diffusion test. a total of 1160 strai ...200010746497
in vitro antibacterial spectrum of a new broad-spectrum 8-methoxy fluoroquinolone, gatifloxacin.the in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin mic90s, < or =1 mg/l, except 4 mg/l against methicillin-resistant staphylococcus aureus and enterococcus faecium). gatifloxacin was two-fold less potent than ciprofloxacin, and the same as or two-fold more potent than ofloxacin against ent ...200010747819
clinical relevance of proteus mirabilis in hospital patients: a two year survey.a retrospective study was performed on 1072 non-duplicate isolates of proteus mirabilis, taken in the period april 1996 to march 1998, and on 100 patient charts randomly selected during the same period. p. mirabilis isolates accounted for 7.7% of enterobacteriaceae. the isolates were predominantly from urine (70.2%); of the total, 38.0% were penicillinase-producing isolates, 6.9% were extended-spectrum beta-lactamase (esbl)-producing isolates and 3.6% produced inhibitor-resistant beta-lactamase ...200010747835
bacterial contamination of bath-water from spinal cord lesioned patients with pressure ulcers exercising in the water.to evaluate the bacterial contamination of the water from the microflora of pressure ulcers in para- and tetraplegic patients, when they were exercising in water at 36 degrees c for half an hour.200010762183
aerobic and anaerobic microbiology of wound infection following spinal fusion in children.to study the aerobic and anaerobic microbiology of wound infections following spinal fusion in children.200010765134
germline and germline mosaic pten mutations associated with a proteus-like syndrome of hemihypertrophy, lower limb asymmetry, arteriovenous malformations and lipomatosis.germline pten mutations cause cowden syndrome (cs) and bannayan-riley-ruvalcaba syndrome (brr), two hamartoma-tumour syndromes, and somatic pten alterations have been shown to participate, to a greater or lesser extent, in a wide variety of sporadic neoplasia. pten is a tumour suppressor and dual-specificity phosphatase which affects apoptosis via its lipid phosphatase activity in the phosphoinositol-3-kinase and akt pathway as well as inhibiting cell spreading via the focal adhesion kinase path ...200010749983
[production of the therapeutic and prophylactic preparation enterobifidin on the basis of bifidobacterium adolescentis mc-42].production of enterobifidin comprises preparation of culture media, reparation of lyophilized bifidobacterium adolescentis ms-42 culture, preparation of starters, cultivation of bacteria in fermenters, biomass conservation, and its biological control. the preparation contains physiologically active bifidobacterium cells with high activities of growth (mu = 0.7 h-1, g = 1.0 h) and acid formation (titratable acidity is approximately 120-140 degrees t; acetate concentration, 0.50-0.75%; and lactate ...200010752094
intracranial complications of acute mastoiditis.oral antibiotic use may have changed the incidence and microbiology of otitic intracranial complications. we reviewed cases of acute mastoiditis to document: (1) incidence of intracranial complications; (2) risk factors; and (3) identify pathologic organisms.200010767461
antimicrobial resistance among urinary tract infection (uti) isolates in europe: results from the sentry antimicrobial surveillance program 1997.the sentry antimicrobial surveillance program was established to monitor the occurrence and antimicrobial susceptibility of bacterial pathogens via an international network of sentinel hospitals. twenty european hospitals referred a total of 887 urinary tract infection (uti) isolates to the european sentry reference laboratory during the period october-december 1997. ninety percent of the referred species were represented by escherichia coli (52%), enterococcus spp. (12%), klebsiella spp. (7%), ...200010768473
[primary bacterial aortitis].primary bacterial aortitis represents a rare disease with a high lethality. from june 1997 to april 1999 5 patients with an abdominal aortic infection were treated by resection of the infected aorta and in-situ reconstruction or by extra-anatomic bypass. there was no treatment in one case because of the infaust prognosis. 3 patients survived, one with a paraparesis as a result of spinal ischemia. on the basis of our patients the pathogenesis, clinical symptoms with diagnosis and the therapeutic ...200010769448
[hypercalcemia due to septic bone metastasis]. 200010776416
structure of the o-specific polysaccharide of proteus mirabilis o11, another proteus o-antigen containing an amide of d-galacturonic acid with l-threonine.the o-specific polysaccharide of proteus mirabilis o11 was studied by sugar analysis, smith degradation, 1h and 13c nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, and 1h-detected 1h, 13c hmqc experiments. the following structure of a pentasaccharide repeating unit of the polysaccharide was established: [formual: see text] where d-gala6lthr is n-(d-galacturonoyl)-l-threonine. elisa with anti-p. mirabilis o11 serum showed that d-gala6lthr is of minor importance for manifesting the ...200010782289
[detection of extended spectrum beta-lactamases producing enterobacteriaceae in feces].a study was made of 366 feces for detection of extended spectrum beta-lactamases producing enterobacteriaceae from feces. the selective agar was used for modified drigalski agar (eiken chemical co., ltd) with 2 micrograms/ml cefotaxime (esbl screen agar). 92 strains of enterobacteriaceae, 41 escherichia coli, 15 citrobacter freundii, 13 enterobacter cloacae, 11 klebsiella pneumoniae, and other 12, were isolated from esbl screen agar. and, r-plasmid that were selected by 2 micrograms/ml cefotaxim ...200010783580
[laboratory diagnosis and serologic course in patients with tularemia].tularemia was practically unknown in spain until the end of 1997, when an epidemic outbreak was declared. this paper presents the data on microbiological diagnosis of 55 patients who suffered from tularemia.200010786358
antibiotic prophylaxis in children with relapsing urinary tract infections: review.recurrent urinary tract infections (utis) are observed in 30-50% of children after the first uti. of these, approximately 90% occur within 3 months of the initial episode. the basic aim of antibiotic prophylaxis in children with malformative uropathy and/or recurrent utis, is to reduce the frequency of utis. the bacteria most frequently responsible for uti are gram-negative organisms, with escherichia coli accounting for 80% of urinary tract pathogens. in children with recurrent utis and in thos ...200010789549
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