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enterobacteria in feedlot waste and runoff.samples of beef cattle feedlot waste (flw), runoff from the pens, and water from a large drainage ditch at the feedlot were examined for enterobacteriaceae. the drainage ditch receives the runoff but contains primarily subsurface drainage from fields on which flw is spread for disposal. planting and enrichment techniques with seven different media were used to isolate 553 cultures of enterobacteria. flw contains about 50 million enterobacteria/g dry weight. more than 90% of these were escherichi ...197216349932
effect of gamma irradiation on the microflora of freshwater fish: ii. generic identification of aerobic bacteria from yellow perch fillets.studies on the generic identification of bacteria isolated from nonirradiated and irradiated (0.3 and 0.6 mrad) yellow perch fillets during the course of microbial spoilage have been conducted. after the enumeration and tabulation of macrocolonies on petri dish cultures obtained from fillets, isolates were examined and keyed out essentially according to modified morphological and biochemical protocols of shewan. identification was further confirmed through reference to bergey's manual. data obta ...196616349702
inhibition of swarming of proteus by sodium tetradecyl sulfate, beta-phenethyl alcohol, and p-nitrophenylglycerine.the effects of sodium tetradecyl sulfate (sts), beta-phenethyl alcohol (pea), and p-nitrophenylglycerol (pnpg) on motility, swarming, flagellation, and growth of proteus were examined. growth-inhibitory concentrations (gic) and swarming-inhibitory concentrations (sic) were determined. a characterization of the swarming-inhibitory efficacy of these compounds was based on their gic/sic ratio and their concentration inhibition curves. using the homologous series of sodium alkyl sulfates as a standa ...196616349688
biochemical and molecular characterization of obesumbacterium proteus, a common contaminant of brewing yeasts.we have evaluated the effectiveness of api 20e, biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (eric)-pcr to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant obesumbacterium proteus. of the five typing techniques, biolog testing, plasmid profiling, and eric-pcr provided the most differentiation, and api 20e testing and ribotyping were relatively indiscriminate. the molecular biology approach of eric-pc ...199416349260
quantitative and qualitative analysis of bacteria in er(iii) solution by thin-film magnetopheresis.magnetic deposition, quantitation, and identification of bacteria reacting with the paramagnetic trivalent lanthanide ion, er, was evaluated. the magnetic deposition method was dubbed thin-film magnetopheresis. the optimization of the magnetic deposition protocol was accomplished with escherichia coli as a model organism in 150 mm nacl and 5 mm ercl(3) solution. three gram-positive bacteria, staphylococcus epidermidis, staphylococcus saprophyticus, and enterococcus faecalis, and four gram-negati ...199316348916
effects of varying concentrations of novobiocin incorporated into two salmonella plating media on the recovery of four enterobacteriaceae.hydrogen sulfide-producing strains of salmonellae, escherichia coli, citrobacter freundii, and proteus mirabilis were isolated from fresh pork sausage. all the strains produced black-centered colonies on hektoen enteric agar (he). on xylose lysine deoxycholate agar (xld), c. freundii produced yellow colonies, and the strains of the other three genera formed black-centered colonies. the selectivity of he and xld for salmonellae was improved by the addition of novobiocin to both media. with increa ...197716345211
assessment of antibacterial activity of some topical otological solutions.otological solutions have long been used in the treatment of the bacterial and fungal infections of the ear. we investigated antibacterial activity of some otic solutions against the most common bacteria isolated from discharging ears.200516340276
effect of triclosan on the development of bacterial biofilms by urinary tract pathogens on urinary catheters.to examine (i) the effect of triclosan on the formation of catheter biofilms by urinary tract pathogens and (ii) the diffusion of triclosan through the retention balloons of urinary catheters.200616339844
prospective study of individuals with long-term urinary catheters colonized with proteus species.to characterize the variability in the times catheters take to block with encrustation in patients who have proteus in their urinary flora, and to identify factors responsible for modulating the rate of catheter encrustation and blockage.200616336341
in-vitro antibacterial and cytotoxic activity of cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes of the antibiotic drug cephalothin (keflin).keflin (kefl) interacts with co(ii), cu(ii), ni(ii) and zn(ii) metal ions leading to complexes of the type m(kefl)2cl2 and m(kefl)cl2, which have been characterized by physicochemical and spectroscopic methods. magnetic moment, ir, electronic spectral and elemental analyses data suggest that keflin behaves tridentately forming octahedral or trigonal bipyramidal complexes with the metal ions mentioned above. the new compounds have been screened in-vitro for antibacterial and cytotoxic activity ag ...200516335054
hsp60 expression during carcinogenesis: a molecular "proteus" of carcinogenesis? 200516333979
in vitro efficacy of the successive or staggered use of eardrops.chronic suppurative otitis media is still a significant health problem. several topical agents such as antibacterials, steroids and acid media eardrops are widely used alone or in combination to control active otorrhea and obtain a dry ear. patients frequently ask if there is any benefit or loss in the effect of these due to their use in a successive or staggered manner. this question led us to design this in vitro study to investigate the effect of the acetic acid solution and dexamethasone sod ...200616328405
evaluation of antimicrobial activity of cleome viscosa and gmelina asiatica.the ethanolic extracts of the leaves and flowers of cleome viscosa and roots of gmelina asiatica were tested for antimicrobial activity. the two plants exhibited a broad spectrum of antimicrobial activity, particularly significative against escherichia coli , proteus vulgaris and pseudomonas aeruginosa. the leaf extract of c. viscosa showed moderate activity against pathogenic fungi.200616325351
case analysis of purple urine-bag syndrome at a long-term care service in a community hospital.purple urine-bag syndrome (pubs) is a rare phenomenon in which the contents of urine bags turn purple or blue following patient catheterization. the condition often causes care givers tremendous distress. we investigated the prevalence and possible causes of pubs for a group of elderly patients.200516323555
comparative antimicrobial potency of meropenem tested against gram-negative bacilli: report from the mystic surveillance program in the united states (2004).the meropenem yearly susceptibility test information collection (mystic) program is a longitudinal resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of bacterial pathogens to carbapenems and other broad-spectrum agents. in 2004 (year six), the antimicrobial activity of 12 broad-spectrum agents was assessed against 2,799 gram-negative bacterial isolates submitted from 15 united states (usa) medical centers using clinical and laboratory standa ...200516323433
interaction between antibiotics and non-specific humoral immunity. 198916312465
sensitivity to beta-lactams and aminoglycosides of clinically important pathogens isolated from children in poland. 198916312442
antimicrobial activity of a new difluorinated quinolone: lomefloxacin (sc-47111). 198916312351
comparative in vitro antibacterial activities of cefetamet (ro 15-8074/005), a new oral cephalosporin, and eight other oral antimicrobials. 198916312332
ytr-830h: its origin, the present and the future. 198916312317
a herd level analysis of urinary tract infection in dairy cattle.cystitis, urethritis and pyelonephritis in cattle most commonly result from ascending urinary tract infection with corynebacterium renale, corynebacterium cystidis, corynebacterium pilosum or escherichia coli. we describe the clinical, bacteriological, clinical-pathological and epidemiological findings in a dairy cattle herd with urinary tract infection (uti). blood and urine samples from 17 calves and 19 cows were submitted to laboratory examinations. depression, muscle wasting, weakness and fr ...200616310382
differential activity of a lectin from solieria filiformis against human pathogenic bacteria.a lectin isolated from the red alga solieria filiformis was evaluated for its effect on the growth of 8 gram-negative and 3 gram-positive bacteria cultivated in liquid medium (three independent experiments/bacterium). the lectin (500 microg/ml) stimulated the growth of the gram-positive species bacillus cereus and inhibited the growth of the gram-negative species serratia marcescens, salmonella typhi, klebsiella pneumoniae, enterobacter aerogenes, proteus sp, and pseudomonas aeruginosa at 1000 m ...200516302091
particularities of bacterial ecology of acute otitis media in an african subtropical country (cote d'ivoire).acute otitis media (aom) is the commonest infectious disease of childhood. its treatment is based on a probabilistic antibiotic therapy.200616297453
microbiology of dacryocystitis among adults population in southern australia.retrospective study on microbiology of dacryocystitis cases in adults was carried out at the royal victorian eye and ear hospital (rveeh). sixty-four consecutive cases seen between march 2000 and june 2003 were included. seventy-nine organisms were isolated from the sixty-six samples (1.2/specimen). aerobic or facultative bacteria were recovered in 76 (96.2), fungi from two specimens (2.5%). and anaerobic bacteria from only one specimen (1.3%). staphylococcus aureus (27), was most common followe ...200516295714
dna sequence analysis of the genetic environment of various blactx-m genes.over a 3 year period (2000-2003) 21 escherichia coli, 5 klebsiella pneumoniae, 1 serratia marcescens and 1 proteus mirabilis producing ctx-m-type beta-lactamase were collected from five different hospitals in paris, france. this study was conducted to analyse the genetic environment of these 28 bla(ctx-m) genes.200616291869
identification and phylogenetic analysis of arsenophonus- and photorhabdus-type bacteria from adult hippoboscidae and streblidae (hippoboscoidea).this is the first report of arsenophonus- and photorhabdus-type bacteria from streblidae (bat flies) and hippoboscidae (louse flies, keds). strains were detected by means of polymerase chain reaction of 16s rdna, and phylogenetic analysis determined the relationship of the obtained sequences to previously reported sequences in genbank. phylogenetic analysis by means of maximum parsimony revealed that all isolated arsenophonus spp. 16s rdna sequences formed a monophyletic sub-clade within other i ...200616289111
occurrence of extended-spectrum (beta)-lactamases and plasmid-mediated ampc (beta)-lactamases among korean isolates of proteus mirabilis. 200616284101
a structured-population model of proteus mirabilis swarm-colony development.in this paper we present continuous age- and space-structured models and numerical computations of proteus mirabilis swarm-colony development. we base the mathematical representation of the cell-cycle dynamics of proteus mirabilis on those developed by esipov and shapiro, which are the best understood aspects of the system, and we make minimum assumptions about less-understood mechanisms, such as precise forms of the spatial diffusion. the models in this paper have explicit age-structure and, wh ...200616283413
relationships between antimicrobial use and antimicrobial resistance in gram-negative bacteria causing nosocomial infections from 1991-2003 at a university hospital in taiwan.this study was conducted to evaluate the relationship between antimicrobial resistance and antimicrobial use in a university hospital in taiwan. disk susceptibility data of escherichia coli, klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, proteus spp., pseudomonas aeruginosa, acinetobacter spp., stenotrophomonas maltophilia and other non-fermentative gram-negative bacilli causing nosocomial infections were evaluated. data on annual patient-days and annual consumption (defined d ...200516280243
lunatusin, a trypsin-stable antimicrobial peptide from lima beans (phaseolus lunatus l.).an anti-fungal peptide designated as lunatusin, with a molecular mass around 7kda, was purified from the seeds of chinese lima bean (phaseolus lunatus l.). the peptide was isolated using a simple protocol consisting of affinity chromatography on affi-gel blue gel and gel filtration on superdex 75. lunatusin exerted an anti-fungal activity toward fungal species such as fusarium oxysporum, mycosphaerella arachidicola and botrytis cinerea, and an antibacterial action on, bacillus megaterium, bacill ...200516269344
the incidence of proteus mirabilis infection increases in patients on treatment but does not trigger disease activity.the aim of this study is to determine whether treatment increases the levels of anti-proteus antibodies (apa) in patients with rheumatoid arthritis (ra). the blood samples of 32 patients suffering from ra who were recruited in our previous study and continued to participate in our follow-up study were collected after 1 year. their first and follow-up samples were analysed for the presence of igg isotype and total immunoglobulins (igg+iga+igm) against proteus mirabalis (pm) using enzyme-linked im ...200616267598
bacterial flora of the low male genital tract in patients consulting for infertility.the physiological aerobic bacterial flora of the low male genital tract was determined. this prospective study was performed on 600 semen specimens collected from 543 asymptomatic males consulting for infertility. semen cultures were sterile in 28.8%, with a polymicrobial flora and/or absence or low titres of ureaplasma urealyticum in 49.3%, and with one or two aerobic and facultative bacteria > or =1 x 10(3) cfu ml(-1) and/or u. urealyticum with titres > or =10(4) ccu ml(-1) (colour changing un ...200516266393
structures of the o-polysaccharides and classification of proteus genomospecies 4, 5 and 6 into respective proteus serogroups.an acidic branched o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide (lps) of proteus genomospecies 4 and studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, roesy and h-detected 1h, 13c hsqc experiments. the following structure of the pentasaccharide repeating unit of the o-polysaccharide was established, which is unique among proteus polysaccharide structures: [structure: see text] where qui3nac stands for 3 ...200516262693
synthesis and evaluation of new quinazolone derivatives of nalidixic acid as potential antibacterial and antifungal agents.in continuation of our work on synthesis of biheterocycles carrying the biodynamic heterocyclic systems at position 3, a series of new nalidixic acid derivatives having quinazolones moiety were synthesised to achieve enhanced biological activity and wide spectrum of activity. nalidixic acid was first converted into its acid chloride using thionyl chloride as an acylating agent at laboratory temperature. later it was converted to methyl ester. nalidixoyl chloride formed vigorously reacts with met ...200616260068
outcome of bacterial culture from mastoid granulations: is it relevant in chronic ear disease?to detect the presence of bacteria in mastoid granulations and compare its prevalence in both types of chronic suppurative otitis media (csom). to find out if stage of disease activity, age, duration of disease, and aditus patency relate to obtaining positive cultures.200516259653
characterization and biological activities of two copper(ii) complexes with diethylenetriamine and 2,2'-bipyridine or 1,10-phenanthroline as ligands.two new mixed ligand copper(ii) complexes with diethylenetriamine, 2,2'-bipyridine and 1,10-phenanthroline have been synthesized. the crystal and molecular structures of [cu(dien)(phen)](clo(4))(2) and [cu(dien)(bipy)](bf(4))(2) (dien=diethylenetriamine, phen=1,10-phenanthroline, bipy=2,2'-bipyridine) were determined by x-ray crystallography from single crystal data. these two complexes have similar structures. the epr spectral data also suggest that these complexes have distorted square pyramid ...200516257723
[dercum's disease: a case report].a case of dercum's disease in a 51-year-old obese woman with a history of brain vasculitis, and painful subcutaneous multiple lipomas is described. this disease, included in the category of rare diseases by the world health organization, first described in 1892 by francis xavier dercum, is characterized by its prevalence among women, its familiarity, by the presence of multiple painful subcutaneous lipomas and its association with obesity, hypercholesterolemia and asthenia. the disease has to be ...200516250185
evolutionarily conserved structural motifs in bacterial gst (glutathione s-transferase) are involved in protein folding and stability.the bacterium proteus mirabilis expresses a cytosolic class beta glutathione s-transferase (pmgst b1-1) that is part of a family of multifunctional detoxication enzymes. like other cytosolic gsts, pmgst b1-1 possesses two local structural motifs, an n-capping box and a hydrophobic staple motif, both of which are located between amino acids 151 and 156. the n-capping box consists of a reciprocal hydrogen bonding interaction of thr152 with asp155, whereas the hydrophobic staple motif consists of a ...200616248855
surveillance of extended-spectrum beta-lactamases from clinical samples and faecal carriers in barcelona, spain.the aim of the present study was to characterize and compare the extended-spectrum beta-lactamase (esbl)-producing organisms isolated from clinical samples and faecal carriers in 2001 and 2002.200516244084
resolving deep phylogenetic relationships in salamanders: analyses of mitochondrial and nuclear genomic data.phylogenetic relationships among salamander families illustrate analytical challenges inherent to inferring phylogenies in which terminal branches are temporally very long relative to internal branches. we present new mitochondrial dna sequences, approximately 2,100 base pairs from the genes encoding nd1, nd2, coi, and the intervening trna genes for 34 species representing all 10 salamander families, to examine these relationships. parsimony analysis of these mtdna sequences supports monophyly o ...200516243763
[necrotizing fasciitis of the perineum secondary to a surgical treatment of bartholin's gland abscess].necrotizing fasciitis of the perineum is a rare but of fast evolution, and potentially fatal infectious disease process. it is characterized by progressive inflammation and extensive necrosis of subcutaneous tissue involving the fascia and other adjacent tissues. this infection may be idiopathic or secondary to local trauma or pelvic surgery. its mortality rate is 20%. we report a case of necrotzing fasciitis of the perineum in a 34-year-old woman following incision and drainage of bartholin's g ...200516243570
antibiotic resistance in outpatient urinary isolates: final results from the north american urinary tract infection collaborative alliance (nautica).the goal of the north american urinary tract infection collaborative alliance (nautica) study was to determine antibiotic susceptibility to commonly used agents for urinary tract infections against outpatient urinary isolates obtained in various geographic regions in the usa and canada. forty-one medical centres (30 from the usa and 11 from canada) participated, with each centre submitting up to 50 consecutive outpatient midstream urine isolates. isolates were identified to species level by the ...200516243229
[effect of the proteolytic enzymes of bacillus licheniformis and the lysoamidase of lysobacter sp. xl1 on proteus vulgaris and proteus mirabilis].preparations of culture liquid of three bacullus licheniformis strains (s, 103, and 60.4) and the enzymatic preparation lysoamidase from culture liquid of lysobacter sp. strain xl1 actively lysed preliminarily autoclaved cells of gram-negative bacteria proteus vulgaris and p. mirabilis. living proteus cells treated with these enzymatic preparations were lysed during their subsequent autoclaving. inoculation of enzyme-treated proteus cells, taken either separately or in combination with one anoth ...200516240656
paraparesis caused by an angiolipomatous hamartoma in an adolescent with proteus syndrome and scoliosis.this 16-year-old boy with proteus syndrome suffered acute spinal cord compression secondary to hemorrhage into an extradural paraspinal angiolipomatous hamartoma. a magnetic resonance (mr) imaging study performed 12 years earlier had revealed the presence of this tumor at the apex of a mild thoracic scoliosis, which had been stable during the intervening decade. spinal cord neurological deficit in proteus syndrome is rare. it can arise either from vertebral abnormalities and kyphoscoliotic defor ...200516238085
photoproduction of hydrogen from raw starch using a halophilic bacterial community.the bacterial community designated bc1, which originates from night soil treatment sludge, exhibited a strong ability to produce h2 from raw starch in the light in the presence of 3% nac1. three halophilic or halotolerant bacterial species, vibrio fluvialis, rhodobium marinum, and proteus vulgaris, were isolated from bc1 and identified. the level of h2 production from starch by coculture of v. fluvialis and r. marinum was nearly equal to that by bc1, indicating that these two strains play roles ...199916232577
antimicrobial activity of some medicinal plants from india.the results of a preliminary antimicrobial screening of the methanol extracts of zingiber officinale, asteracantha longifolia, citrus acida, salacia microsperma and tinospora cordifolia are reported.200516229969
antibacterial and wound healing properties of methanolic extracts of some nigerian medicinal plants.this study was conducted to evaluate methanolic extracts of ageratum conyzoides, anthocleista djalonensis, napoleona imperialis, ocimum gratissimum, and psidium guajava for antibacterial and wound healing properties. antibacterial properties of the extracts were studied against eleven wound isolates (staphylococcus aureus (four strains), e. coli (two strains), pseudomonas aeruginosa (one strain), proteus spp. (three strains), and shigella spp. (one strain)) using the well diffusion method. wound ...200616226414
extended spectrum beta-lactamases among gram-negative bacteria of nosocomial origin from an intensive care unit of a tertiary health facility in tanzania.resistance to third generation cephalosporins due to acquisition and expression of extended spectrum beta-lactamase (esbl) enzymes among gram-negative bacteria is on the increase. presence of esbl producing organisms has been reported to significantly affect the course and outcome of an infection. therefore infections due to esbl isolates continue to pose a challenge to infection management worldwide. the aim of this study was to determine the existence and to describe phenotypic and genotypic c ...200516225701
plasma and urinary adrenomedullin levels in children with acute pyelonephritis.adrenomedullin (am), a novel peptide recently isolated from pheochromocytoma, eliciting vasorelaxing activity, is the strongest among all known peptides. am has been detected in the adrenal medulla, cardiac tissue, lung and kidney. immunohistochemical studies have demonstrated the localization of am in glomeruli, tubules and collecting cells of the kidney. clinically, plasma and urinary am levels are altered in patients with different renal disease. the present study aims to determine plasma and ...200516221100
[prevalence and risk factors for bacteriuria in patients with cirrhosis].bacterial infections occur frequently in patients with cirrhosis and may worsen the disease outcome. we investigated the prevalence of bacteriuria in 500 consecutive patients with cirrhosis, in different child-pugh stages (41.4% a; 40.8% b; 17% c) and analysed the associated risk factors. most of the cirrhosis cases were virus related; alcohol abuse was recorded in 6.2% of the patients. bacteriuria was detected in 139 (27.8%) cases: 32.4% were more than 100,000 cfu/ml; 7.9% between 100,000 and 1 ...200516220030
increase of faecal bifidobacteria due to dietary oligosaccharides induces a reduction of clinically relevant pathogen germs in the faeces of formula-fed preterm infants.in a previous study on formula-fed preterm infants, we were able to demonstrate that dietary oligosaccharides (a mixture of 90% galacto-oligosaccharides and 10% fructo-oligosaccharides in a concentration of 1 g/dl) stimulate the growth of faecal bifidobacteria. in the present explorative analysis of this study, we focus on the effect of the dominance of bifidobacteria on the presence of clinically relevant pathogens (staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, ...200516214763
n,n-dichlorotaurine: chemical and bactericidal properties.the biogenous antimicrobial agent n-chlorotaurine (nct) converts by disproportionation to n,n-dichlorotaurine (ndct) at a rate proportional to acidity. this occurs at appreciable amounts already in weakly acidic biological systems. to understand the consequences of ndct formation, a thorough investigation of this undescribed compound was mandatory, which needed its synthesis. differently from nct, this was possible in the aqueous system using trichloroisocyanuric acid. while the free acid, cl(2) ...200516211659
in-vitro antibacterial, antifungal and cytotoxic activities of some coumarins and their metal complexes.a series of new antibacterial and antifungal coumarin-derived compounds and their transition metal complexes [cobalt (ii), copper (ii), nickel (ii) and zinc (ii)] have been synthesized, characterized and screened for their in vitro antibacterial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebacterium diphtheriae, staphylococcus aureus and streptococcus pyogenes bacterial strains ...200516206827
metabolic activation of bladder procarcinogens, 2-aminofluorene, 4-aminobiphenyl, and benzidine by pseudomonas aeruginosa and other human endogenous bacteria.pseudomonas aeruginosa, an opportunistic pathogen of the human urinary tract, and other selected human endogenous bacteria were investigated for metabolic activation of the bladder procarcinogens, 2-aminofluorene (2-af), 4-aminobiphenyl (4-ab), and benzidine (bz). the cell-free extracts of pseudomonas aeruginosa, escherichia coli, enterobacter aerogenes, proteus mirabilis, proteus vulgaris, staphylococcus epidermidis, staphylococcus saprophyticus, klebsiella pneumoniae, and intestinal anaerobes, ...200616203120
multiple origins and incursions of the atlantic barnacle chthamalus proteus in the pacific.chthamalus proteus, a barnacle native to the caribbean and western atlantic, was introduced to the pacific within the last few decades. using direct sequencing of mitochondrial dna (coi), we characterized genetic variation in native and introduced populations and searched for genetic matches between regions to determine if there were multiple geographical sources and introduction points for this barnacle. in the native range, we found great genetic differences among populations (max. f(st) = 0.6 ...200516202091
identification and antimicrobial susceptibility of micro-organisms recovered from cutaneous lesions of human american tegumentary leishmaniasis in minas gerais, brazil.an evaluation of the microbiota present in cutaneous ulcers from 31 patients with a clinical and parasitological diagnosis of american tegumentary leishmaniasis (atl) was carried out by the standard filter paper disc technique, including antimicrobial susceptibility of the bacterial isolates. microbial examination indicated that 21 patients (67.7%) were contaminated with one to four bacteria and some of them also with yeast. a total of 142 micro-organisms were isolated. staphylococcus aureus was ...200516192439
granulomatous inflammation of salt glands in ducklings (anas platyrhynchos) associated with intralesional gram-negative bacteria.the "nasal glands" occur in many bird species and are powerful sodium ion-excretory organs. in ducks, they are located in supraorbital bony recesses. granulomatous inflammation of these glands occurs with an incidence of approximately 1% in ducklings (anas platyrhynchos), and is not associated with specific clinical symptoms. we investigated nine glands of eight animals with granulomas by gross pathology and histopathology, and compared results of bacteriology with 20 non-lesioned nasal glands. ...200516191707
in vitro and in vivo antibacterial activities of sm-216601, a new broad-spectrum parenteral carbapenem.sm-216601 is a novel parenteral 1beta-methylcarbapenem. in agar dilution susceptibility testing, the mic of sm-216601 for 90% of the methicillin-resistant staphylococcus aureus (mrsa) strains tested (mic(90)) was 2 microg/ml, which was comparable to those of vancomycin and linezolid. sm-216601 was also very potent against enterococcus faecium, including vancomycin-resistant strains (mic(90) = 8 microg/ml). sm-216601 exhibited potent activity against penicillin-resistant streptococcus pneumoniae, ...200516189097
bacteriology of surgical wound infections in a tertiary care hospital in turkey.to determine the spectrum of the pathogens cultured from surgical wound infections and assess their antimicrobial drug resistances.200516167704
influence of human ascitic fluid on the in vitro antibacterial activity of moxifloxacin.we investigated the in vitro influence of haf on the antibacterial activity of moxifloxacin against escherichia coli atcc 10798, escherichia coli k-12, proteus rettgeri (sanelli), staphylococcus aureus atcc 25923, staphylococcus aureus nctc 1808 and staphylococcus epidermidis atcc 12228. human ascitic fluid was obtained from 6 cirrhotic patients by paracentesis. the interaction effect was evaluated by the checkerboard technique. our results indicate the ability of human ascitic fluid to reduce m ...200516167519
genotyping of urinary and fecal proteus mirabilis isolates from individuals with long-term urinary catheters. 200516167137
phylogeny of the enterobacteriaceae based on genes encoding elongation factor tu and f-atpase beta-subunit.the phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor tu gene (tuf) and of their f-atpase beta-subunit gene (atpd). an 884 bp fragment for tuf and an 884 or 871 bp fragment for atpd were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. the atpd sequence analysis exhibited an indel specific to pantoea and tatumella species, showing, for the first time, a tight phylogenetic ...200516166704
the ability of proteus mirabilis to sense surfaces and regulate virulence gene expression involves flil, a flagellar basal body protein.proteus mirabilis is a urinary tract pathogen that differentiates from a short swimmer cell to an elongated, highly flagellated swarmer cell. swarmer cell differentiation parallels an increased expression of several virulence factors, suggesting that both processes are controlled by the same signal. the molecular nature of this signal is not known but is hypothesized to involve the inhibition of flagellar rotation. in this study, data are presented supporting the idea that conditions inhibiting ...200516166542
toxicological effect of indole and its azo dye derivatives on some microorganisms under aerobic conditions.azo dyes are ubiquitous commercial chemicals that present unique environmental problems. the azo dyes in particular can undergo natural anaerobic degradation to potentially carcinogenic amines. they pose a major problem for water-treatment plants downstream. one strategy to remidate polluted surface contaminants is to make use of the degradative capacity of bacteria rather than using destructive chemical reactions. therefore, pathogenic and non-pathogenic microorganisms namely; bacillus thuringi ...200616165191
regional variation in the prevalence of extended-spectrum beta-lactamase-producing clinical isolates in the asia-pacific region (sentry 1998-2002).we examined the prevalence of extended-spectrum beta-lactamase (esbl)-producing strains of klebsiella pneumoniae, klebsiella oxytoca, escherichia coli, proteus mirabilis, citrobacter koseri, and salmonella spp. that were isolated as part of the sentry asia-pacific surveillance program between 1998 and 2002. during the study period, a total of 6,388 strains were gathered from 17 medical centers in 7 countries and examined for esbl production and hyperproduction of k. oxytoca chromosomal k1 beta-l ...200516165001
[surveillance of susceptibility of clinical isolates to cefmetazole between 2000 and 2003].for the post-marketing surveillance of cefmetazole (cmz, cefmetazon), mics of injectable beta-lactam antibacterials including cmz against clinical isolates from 15 medical institutions all over japan are measured yearly and the incidence rates of resistance in various species are also evaluated. in the first surveillance from june 2000 to march 2001, 574 isolates of 13 species were tested, 548 isolates of the same 13 species were tested in the second surveillance from april 2001 to march 2002, a ...200516161756
anti-bacterial activity of euphorbia fusiformis--a rare medicinal herb.euphorbia fusiformis buch.-ham. ex. d.don (euphorbiaceae) is a rare medicinal herb. aqueous and organic solvent extracts of the leaves and rootstocks were investigated for anti-bacterial properties by using disc diffusion and well-in agar methods, against pathogenic strains of gram positive (bacillus subtilis and staphylococcus aureus) and gram negative bacteria (escherichia coli, klebsiella pneumoniae, proteus vulgaris, pseudomonas aeruginosa, salmonella typhii a and salmonella typhii b). the d ...200516159702
[efficacy of probiotic bactisporin in therapy of intrahospital urinary infection].we studied efficacy of bactisporin (probiotic based on aerobic spore-forming culture bacillus subtilis, strain 3h) in combined treatment of intrahospital urinary infection (iui) in 36 patients with infravesical obstruction. these patients developed postoperative complications due to iui caused by antibiotic-resistant hospital flora. the control group consisted of 35 patients matched by age and disease given conventional postoperative etiotropic therapy. bacterial translocation enables bactispori ...200516158748
purple urine bag syndrome in a hemodialysis patient.purple urine bag syndrome (pubs) is an uncommon disorder, in which the plastic disposable urinary catheter bag turns purple or blue following hours or days of urinary catheterization. the purple discoloration results from indirubin dissolved in the plastic mixing with indigo in the urine. bacteria possessing indoxyl sulfatase degrade indoxyl sulfate into indirubin and indigo. indoxyl sulfate is derived from the metabolism of tryptophan. pubs usually occurs in chronic catheterized elderly women w ...200516157987
extended spectrum beta-lactamase-producing klebsiella pneumoniae chronic ambulatory peritoneal dialysis peritonitis treated successfully with polymyxin b.peritonitis is not an infrequent complication of inpatients with chronic ambulatory peritoneal dialysis (capd). capd peritonitis may be related to the catheter or secondary to perforation of an intra-abdominal viscus. the most common organisms usually associated with capd peritonitis are staphylococcus aureus and staphylococcus epidermidis (coagulase-negative staphylococci). rarely, aerobic gram-negative bacilli have been the causative agents of capd peritonitis. the treatment of capd peritoniti ...200516157192
hemihyperplasia with ehlers-danlos syndrome like skin changes.hemihyperplasia is a condition characterized by asymmetric overgrowth of one or more body parts. it may be an isolated finding or may be associated with various syndromes like beckwith-weidemann syndrome, klippel-trenaunay-weber syndrome, proteus syndrome etc. pigmentary skin lesions and capillary malformations are commonly seen in cases of hemihyperplasia. we report a case of hemihyperplasia with associated skin hyperextensibility, varicose veins, skin fragility, easy bruising and papery scars, ...200516155424
predictive modelling of growth and measurement of enzymatic synthesis and activity by a cocktail of selected enterobacteriaceae and aeromonas hydrophila.the possibility was examined of developing a predictive model that would predict food spoilage by combining microbial growth (increase in cellular number) with extracellular enzymatic activity of a cocktail of five strains of enterobacteriaceae: escherichia coli, enterobacter agglomerans, klebsiella oxytoca, klebsiella pneumoniae and proteus vulgaris and one aeromonas hydrophila strain. estimations of growth and enzyme activity were made within a three-dimensional matrix of conditions: temperatu ...200516154655
intestinal protozoa and associated bacteria in captive houbara bustards (chlamydotis undulata) in the united arab emirates.a protozoal survey was carried out in 114 captive houbara bustards (chlamydotis undulata) in the united arab emirates. trichomonas gallinarum, chilomastix gallinarum, giardia spp. and lophomonas spp. were detected from faecal samples of 49 (43%) captive houbara bustards. culture and identification studies were carried out to assess associated bacteria. the bacteria isolated from birds negative to protozoa included enterococcus spp., aerococcus spp., micrococci spp., proteus spp., enterobacter sp ...199916147554
analysis of antibiotic susceptibility and resistance of leg ulcer bacterial flora in patients hospitalized at dermatology department, poznań university hospital.effective treatment of frequently infected, poorly healing wounds such as leg ulcers due to chronic venous insufficiency poses a major clinical problem. antibiotic resistance in dermatology patients, especially those with non-healing leg ulcers, is a widespread phenomenon. various antibiotics, mainly broad-spectrum agents, are frequently and sometimes inappropriately prescribed, which often leads to the selection of antibiotic-resistant bacteria strains. the aim of this study was to analyze anti ...200516146620
bacterial flora of leg ulcers in patients admitted to department of dermatology, poznań university of medical sciences, during the 1998-2002 period.venous leg ulcers are an important cause of morbidity in a significant percentage of the world population. the percentage of leg ulceration, either active or healed, in the european population is about 1%-2%. the aim of this study was to analyze the rate of colonization and qualitative composition of the bacterial flora isolated from leg ulcers in patients admitted to dermatology department, poznań university of medical sciences, during the 1998-2002 period, with special reference to the infecti ...200516146619
recurrent urinary tract infections--uropathogen incidence and drug sensitivity.the aim of the study was the retrospective analysis of the results of the drug sensitivity studies of bacterial species cultured from 681 urinary specimens collected from 79 children (12 boys and 67 girls) aged 0-18 years with recurrent urinary tract infection in a period from 1997 to 2001. factors predisposing to recurrent urinary tract infections and the incidences of particular uropathogens were also analyzed. escherichia coli (57.3%), proteus spp. (15.6%) and pseudomonas aeruginosa (9.1%) we ...200416146092
the susceptibility of certain bacterial strains to imidazotriazine derivatives.the synthesized imidazo[2,1-c]triazines were tested for their antimicrobial activity. microbiological screening tests conducted in relation to 54 strains of gram-positive and 52 strains of gram-negative bacteria, 6 strains of yeast-like fungi and 3 strains of moulds showed significant activity of compound 19 against 52 gram-negative bacterial strains. this compound was active against 16 strains of escherichia coli, 12 strains of pseudomonas, 10 strains of proteus spp., 8 strains of klebsiella pn ...200416146057
[bacterial flora and mycosis of the vagina in women with symptoms of vaginal inflammation].to estimate the microbiological profile of vaginal flora in 30 women with gynecologic problems and 20 pregnant women complaining about pathological symptoms {pruritus, burning, vaginal discharge}.200516145858
[antibiotic resistance: from the results of onerba's networks to good antibiotic use]. 200516144065
rheumatoid arthritis and proteus. 200516138503
antibacterial activity of zuccagnia punctata cav. ethanolic extracts.the present study was conducted to investigate antibacterial activity of zuccagnia punctata ethanolic extract against 47 strains of antibiotic-resistant gram-negative bacteria and to identify bioactive compounds. inhibition of bacterial growth was investigated using agar diffusion, agar macrodilution, broth microdilution and bioautographic methods. zuccagnia punctata extract was active against all assayed bacteria (escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, ...200516137849
[health accuracy of travnik cheese and quality standards of sanitary accuracy].the aim of work was to determine a health accuracy of sheep milk and the final product travnik cheese, as well as the quality standards of sanitary accuracy. the study was performed on 212 milk samples selected by random choice. the study involved observing the sanitary conditions of sheep milk and cheese production, as well as isolation and identification of causal agents of infective diseases in these products. the results showed the following agents isolated from milk and travnik cheese: esch ...200516134761
[comparison of frequency and susceptibility to antimicrobials of bacterial strains of the family enterobacteriaceae isolated from patients hospitalised in intensive care units].the aim of the study was estimation of frequency and susceptibility to antimicrobial agents of gram-negative rods isolated from clinical specimens obtained from patients requiring intensive care, with emphasis on profile of the unit. the analysis comprised strains of gram-negative rods isolated from patients of two intensive care units (icus) of a tertiary care hospital (1200 beds). identification of cultured isolates was done using automated vitek and api systems (biomerieux, france). susceptib ...200516134390
[prevalence of multidrug-resistant proteus spp. strains in clinical specimens and their susceptibility to antibiotics].proteus sp. are opportunistic microorganisms which cause urinary tract and wounds infections, bacteriaemia and sepsis. the aim of this study was analysis of prevalence of multidrug-resistant proteus sp. strains in clinical specimens and evaluation of their susceptibility to selected antibiotics. the study was carried out of 1499 proteus sp. strains were isolated in 2000-2003 from patients of departments and dispensaries of the university hospital cm in bydgoszcz umk in torun. the strains were id ...200516134389
the incorporation of glucosamine into enterobacterial core lipopolysaccharide: two enzymatic steps are required.the core lipopolysaccharide (lps) of klebsiella pneumoniae is characterized by the presence of disaccharide alphaglcn-(1,4)-alphagala attached by an alpha1,3 linkage to l-glycero-d-manno-heptopyranose ii (ld-heppii). previously it has been shown that the wabh enzyme catalyzes the incorporation of glcnac from udp-glcnac to outer core lps. the presence of glcnac instead of glcn and the lack of udp-glcn in bacteria indicate that an additional enzymatic step is required. in this work we identified a ...200516131489
synthesis and antimicrobial activity of some novel derivatives of benzofuran: part 1. synthesis and antimicrobial activity of (benzofuran-2-yl)(3-phenyl-3-methylcyclobutyl) ketoxime derivatives.the reaction of salicylaldehyde with 1-phenyl-1-methyl-3-(2-chloro-1-oxoethyl) cyclobutane (1) and potassium carbonate was used to prepare (benzofuran-2-yl)(3-methyl-3-phenylcyclobutyl) methanone (2) for the starting reagent purposes. (benzofuran-2-yl)(3-phenyl-3-methyl cyclobutyl) ketoxime (3) was synthesized from the reaction of the compound (2) with hidroxylamine. new derivatives of (benzofuran-2-yl)(3-phenyl-3-methyl cyclobutyl) ketoxime (3) such as, o-glycidylketoxime (4) and o-phenylacylke ...200516129517
a strategy for the control of catheter blockage by crystalline proteus mirabilis biofilm using the antibacterial agent triclosan.catheter blockage by crystalline proteus mirabilis biofilm is a common complication in patients undergoing long-term indwelling bladder catheterisation. previously we have shown that inflating the retention balloons of all-silicone catheters with triclosan solutions prevents the encrustation process. the aim of the present work was to examine whether this strategy is effective in latex-based catheters.200516126323
bacteria isolated from blood, stool and urine of typhoid patients in a developing country.blood, stool and urine samples were collected from 100 patients diagnosed as having typhoid in 5 hospitals in akwa ibom state and analyzed for the presence of salmonella species and other bacteria. of the 100 blood samples screened, 55 (55%) were positive with the widal test and 39 (39%) were positive on blood culture. thirteen (14.1%) out of 92 urine samples were positive for bacterial growth, while 22 (26.8%) of the stool cultures were positive out of the 82 samples screened. those within the ...200516124436
development and evaluation of 16s rdna microarray for detecting bacterial pathogens in cerebrospinal fluid.the rapid identification of bacteria in cerebrospinal fluid (csf) is very important for patient management and antimicrobial therapies. we developed a 16s dna microarray-based method that targets 16s rdna and can directly detect bacteria from csf without cultivation. universal primers and specific probes were designed from the 16s rdna sequence data retrieved directly from the genbank database. the specificity of the assay is obtained through a combination of microarray hybridization and enzymat ...200516118409
a study on inhibitory effects of siğla tree (liquidambar orientalis mill. var. orientalis) storax against several bacteria.in this study, siğla (liquidambar orientalis mill.) storax (styrax) was investigated for antibacterial activity against aeromonas hydrophila, bacillus amyloliquefaciens, b. brevis, b. cereus, b. megaterium, b. subtilis, corynebacterium xerosis, enterobacter aerogenes, enterococcus faecalis, escherichia coli, e. coli o157:h7, klebsiella pneumoniae, listeria monocytogenes, micrococcus luteus, mycobacterium smegmatis, proteus vulgaris, pseudomonas aeruginosa, p. fluorescens, staphylococcus aureus a ...200516114094
[weil-felix test]. 200516111212
biochemical characterization of the chondroitinase abc i active site.cabc i (chondroitinase abc i) from proteus vulgaris is a galag (galactosaminoglycan) depolymerizing lyase that cleaves its substrates at the glycosidic bond via beta-elimination. cabc i cleaves a particularly broad range of galag substrates, including cs (chondroitin sulphate), ds (dermatan sulphate) and hyaluronic acid. we recently cloned and recombinantly expressed cabc i in escherichia coli, and completed a preliminary biochemical characterization of the enzyme. in the present study, we have ...200516108757
potency and spectrum of tigecycline tested against an international collection of bacterial pathogens associated with skin and soft tissue infections (2000-2004).the antimicrobial activity of tigecycline, a novel glycylcycline, was evaluated against 5289 bacterial isolates recovered from hospitalized patients with skin and soft tissue infections during 2000-2004. strains were submitted from >70 medical centers in north america, latin america, and europe, and were tested centrally using reference broth microdilution methods. the top 10 ranking pathogens (95% of total) recovered included staphylococcus aureus (55.2%), enterococcus spp. (9.6%), pseudomonas ...200516105564
the family of serratia type pore forming toxins.the serratia marcescens hemolysin represents the prototype of a growing family of pore forming toxins. the available bacterial genome sequences reveal serratia hemolysin homologues in additional species. however, only s. marcescens hemolysin has been studied in great molecular detail. this family of toxins has nothing in common with the pore forming toxins of e. coli type (rtx toxins), the staphylococcus aureus alpha-toxin or the thiol activated toxin of group a beta-hemolytic streptococci (stre ...200516101433
[the effect of selective bowel decontamination and mechanical bowel preparation on bacterial translocation due to intraabdominal hypertension].the objective of our study is to evaluate the preventive effects of selective digestive decontamination (sdd) and mechanical bowel preparation in rats with experimentally induced bacterial translocation.200516100664
proteus syndrome: a rare cause of hemihypertrophy and macrodactyly on bone scanning.proteus syndrome is a rare, sporadic genetic disorder characterized by overgrowth of multiple different tissues in a mosaic pattern. it is associated with connective tissue nevi, epidermal nevi, disproportionate overgrowth of multiple tissues, vascular malformations, characteristic tumors, and specific facial anomalies. joseph merrick, popularly known as the elephant man, is now believed to have suffered from proteus syndrome. a case of proteus syndrome and associated findings on bone scintigrap ...200516100477
efficacy of dilute betadine solution irrigation in the prevention of postoperative infection of spinal surgery.prospective, single-blinded, randomized study.200516094267
an electrified catheter to resist encrustation by proteus mirabilis biofilm.we investigated the effect of iontophoresis produced by passing an electric current through silver electrodes attached to catheters on catheter encrustation by crystalline proteus mirabilis biofilm.200516094079
frequency and treatment of urinary tract infection in children subjected to urine culture, in sana'a, yemen.this study was carried out to estimate the frequency of urinary tract infection among children subjected to urine culture presenting to sam hospital, sana'a city, yemen and to determine the susceptibility of the isolated bacteria to the antibiotics.200516092643
risk of nosocomial bacteria transmission: evaluation of cleaning methods of probes used for routine ultrasonography.there exists a small but definite risk of nosocomial infection transmission attributable to ultrasonography probes and coupling gels.200516092321
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