Publications

proceedings: bacteriology of ileal conduits.		1975	1148640
the tryptophanase from proteus rettgeri, improved purification and properties of crystalline holotryptophanase.	the inducible tryptophanase (l-tryptophan indole-lyase (deaminating) ec 4.1.99.1) was crystallized in holoenzyme from the cell extract of proteus rettgeri. the purification procedure included ammonium sulfate fractionation, heat treatment at 60 degrees c, deae-sephadex and hydroxylapatite column chromatographies. crystallization was performed by the addition of ammonium sulfate to the purified enzyme solution containing 20% (v/v) glycerol, 0.1 mm pyridoxal phosphate and 10 mm mercaptoethanol. th ...	1975	1148216
acute postoperative wound complications after gastric surgery for morbid obesity.	the acute postoperative wound complications of 225 morbidly obese patients undergoing gastric bypass were compared against the complications observed in 225 normal-sized individuals who underwent elective gastric resection for peptic ulcer disease. not only were there more wound infections (8.9 percent to 5.3 percent) in the obese population, but these infections inflicted more serious complications and a significantly increased hospital stay. while the exact cause of the increase in infections ...	1975	1147403
faecal stasis and bacteriuria: experimental research in rats.		1975	1145908
[change of the hereditary properties of amoeba proteus during micrurgic intervention at the time of mitosis (metaphase ane early anaphase)].	studies have been made on viability and hereditary properties of the progeny of amoebae after micrurgical coercions (sectioning of cells, suction of cytoplasm) during metaphase and early anaphase. the progeny of the fragments as well as of the cells with the reduced bulk of the cytoplasm exhibits unusual methionine resistance. possible mechanism of the observed phenomemon are discussed.	1975	1145767
oriented thick and thin filaments in amoeba proteus.	actin and myosin filaments as a foundation of contractile systems are well established from ameba to man (3). wolpert et al. (19) isolated by differential centrifugation from amoeba proteus a motile fraction composed of filaments which moved upon the addition of atp. actin filaments are found in amebas (1, 12, 13) which react with vertebrate heavy meromyosin (hmm), forming arrowhead complexes as vertebrate actin (3, 9), and are prominent within the ectoplasmic tube where some of them are attache ...	1975	1141376
canine polypoid cystitis.	the clinical, laboratory, and radiographic characteristics of polypoid cystitis in 2 dogs were similar to those commonly associated with neoplasms of the urinary bladder. gross appearance of the polyps did not permit differentiation between inflammation and neoplasia; microscopic examination of excised tissue was required to establish a definitive diagnosis. following surgical extirpation of the polyps, remission of clinical signs was induced by prolonged antibacterial therapy.	1975	1141031
amoeba proteus: the nuclear periphery.	this study extends previous work on the nuclear envelope and associated structures. it illustrates that the cylindrical structures of the honeycomb lattice are not attached to the nuclear envelope, although generally perpendicular and closely apposed to it, and that there is a complex arrangement of fibrillar material between the cylinders of the lattice. the relationship of nuclear helices to these structures is described and the possible mode of their transfer from nucleus to cytoplasm is disc ...	1975	1137851
trimethoprim-sulfamethoxazole in chronic bronchitis.	authenic tracheobronchial secretions/exudates (tbse) were aspirated under direct vision via a sterile catheter passed through a fiberoptic bronchoscope from patients with chronic obstructive pulmonary disease complicated by chronic bronchitis. tbse, saliva and blood were obtained during long-term administration of trimethoprim-sulfamethoxazole (tmp-smx) and were assayed for drug content. before and during treatment tbse were cultured qualitatively and quantitatively for aerobic and anaerobic bac ...	1975	1137833
susceptibility of clinical isolates of enterobacteriaceae to bl-s640, a new oral cephalosporin.	the in vitro activity of bl-s640, a 7-(2-aryl-2-aminoacetamido)-3-(heterocyclic-thiomethyl) cephalosporin, was evaluated against 338 clinical isolates of enterobacteriaceae in comparison with ampicillin, cephalothin, cefazolin, and cephalexin. against escherichia coli, bl-s640 was as active as cefazolin and more active than ampicillin, cephalothin, and cephalexin. bl-s640 was as effective as the other cephalosporins tested and far more active than ampicillin against klebsiella and was more activ ...	1975	1137392
bl-s 640, a cephalosporin with a broad spectrum of antibacterial activity: properties in vitro.	bl-s 640 was evaluated in vitro by comparison with cephalothin, cephaloridine, cefazolin, and cephalexin. the new compound was more active than the control cephalosporins against most major gram-negative and some gram-positive species. moreover, its antibacterial spectrum included strains of enterobacter, proteus morganii, p. rettgeri, and providencia stuartii, species generally resistant to the other cephalosporins. bl-s 640 was an effective bactericidal agent for strains of various species of ...	1975	1137381
activity of three aminoglycosides and two penicillins against four species of gram-negative bacilli.	three aminoglycoside antibiotics and two penicillins were compared for their in vitro activity against 60 isolates of serratia, pseudomonas, proteus mirabilis, and indole-positive proteus sp. testing was done by the agar dilution method using mueller-hinton broth solidified with 1.5% agar. the activity of amikacin, aminodeoxybutirosin, and gentamicin against proteus and pseudomonas, as related to their peak blood levels, showed no significant differences. amikacin was the most active against ser ...	1975	1137369
microfibrillar structures in the nucleus and cytoplasm of amoeba proteus.	the presence of microfibrillar structures in the nucleus and cytoplasm of amoeba proteus has been described after glutaraldehyde and osmium fixation. the possible roles of cytoplasmic microfibrils in the contraction process of amoeba and nuclear microfibrils in the formation of the honeycomb nuclear lamina are discussed.	1975	1133571
effects of induced pinocytotic activity and extreme temperatures on the morphology of golgi bodies in amoeba proteus.	the morphology of the golgi apparatus of amoeba proteus can be influenced by substances inducing pinocytotic activity as well as by extreme temperatures. during the ingestion of a solution of 0.5% egg white the number of golgi bodies decreases from 100% measured in control cells to 82% measured in cells showing induced pinocytosis. simultaneously the ratio of the surface area of the cisternae at the proximal face to that of the vesicles at the distal face of single dictyosomes remains constant ...	1975	1131858
nephrotoxicity of combined cephalothin-gentamicin regimen.	two patients developed acute tubular necrosis, characterized clinically by acute oliguric renal failure, while they were receiving a combination of cephalothin sodium and gentamicin sulfate therapy. patients who are given this drug regimen should be observed very carefully for early signs of nephrotoxicity. high doses of this antibiotic combination should be avoided especially in elderly patients. patients with renal insufficiency should not be given this regimen.	1975	1130930
experience with detailed surveillance of nosocomial infection.	infection rates have been determined in a 332 bed university hospital using an efficient surveillance program. this computerized program analyzed, summarized and returned infection reports in six days. the efficiency rate of this system was 80 per cent as determined by prevalence survey. the mean nosocomial infection rate for the 12 month study period was 10.8 per cent. neurology, medicine, neurosurgery, surgery and urology ahd the highest attack rates. urinary tract infections composed 44 per c ...	1975	1129686
in vitro antibacterial activity of amikacin, a new aminoglycoside, against clinical bacterial isolates from children.	four hundred and fifty-eight clinical bacterial isolates from a children's hospital were examined for antibiotic susceptibility to amikacin (bb-ks) in comparison with a number of other antibiotics by the disk diffusion and agar dilution methods. the wide spectrum of activity of amikacin against gram-negative bacteria was confirmed; it included e. coli, proteus species, enterobacter species, and pseudomonas aeruginosa. staphylococci were highly sensitive, but other gram-positive bacteria tested w ...	1975	1127131
infections in total hips secondary to a primary source elsewhere.	this is a report of 3 cases in which a primary infection in a site other than a total hip led to infection in the hip itself. one hip infection appeared to arise in a tooth abscess, a second in the urinary tract, and one from the respiratory tract. all infections resulted in the necessity of removing the components of the total hip. it is suggested that patients who have had previous total hip replacement should be warned to consult their physician when the possibility of an infection is present ...	1975	1126095
[intrageneric o-antigenic relations of providencia bacteria].	the authors present literature and personal data on the intragenera o-antigenic relations of the strains of a collection of bacteria belonging to providencia genus, including standard strains of 53 serological o-groups whose antigenic interrelations were studied serologically in the tests of agglutination and adsorption of agglutinins. experimental results confirmed the presence of unilateral relations by the o-antigen between the serological groups 01-021, 03-021 and bilateral relations between ...	1975	1124619
the tryptophanase from proteus rettgeri: sulfhydryl groups in the holo- and apo-enzyme preparations.		1975	1123078
[effect of carbenicillin and its combination with prodigiozan and lysozyme on the course of an experimental inflammatory process of proteus etiology].	biological properties of 142 proteus strains isolated from patients were studied. sensitivity of proteus to ii antibiotics was tested. the isolates were resistant to most of the antibiotics. the highest number of the isolates was sensitive to ampicillin (77.1 minus or plus 7.16) and especially to carbenicillin (82.6 plus or minus 6.16). this provided the use of carbenicillin for the treatment of experimental septicemia in albino mice and wound processes in rabbits with proteus complications. the ...	1975	1119792
effects of procedural variations on the activity of aminoglycosides in vitro.	am j clin pathol 63:438-445, 1975. the effects of procedural variations on the activities of gentamicin, tobramycin, sisomicin, kanamycin, and amikacin in vitro were evaluated using 134 clinical isolates. in broth dilution studies, a change in assay medium from brain-heart infusion broth to mueller-hinton broth resulted in significant changes in minimal inhibitory concentrations in 36% (242 of 670) of assays. a change in the bacterial population size utilized in broth dilution studies resulted i ...	1975	1119441
[necrotic and infected tubal cancer. ileo-sigmoid fistula].		1975	1118696
a transvesical approach to total seminal vesiculectomy: a case report.		1975	1117507
tryptophan biosynthetic pathway in the enterobacteriaceae: some physical properties of the enzymes.	several physical properties of the first four enzymatic activities of the tryptophan pathway were examined using gel filtration and ion exchange chromatography. five different patterns were noted. differences in the anthranilate synthetase (as) and phosphoribosylanthranilate transferase (prt) defined these patterns. in all the organisms studied phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase co-eluted from both diethylaminoethyl-cellulose and g-200 and thus probably ...	1975	1116988
antibiotics in surgery.		1975	1115614
[recent studies on the in vivo and in vitro activity of gentamicin].	the effect of gentamycin was investigated at the university of modena phthisiology and respiratory diseases department. in vitro activity against most pathological gram-positive and gram-negative bacteria was observed. a dose of 4-5 mg/kg proved very effective in cases of bacteria-induced bronchopneumopathy. the drug was also active against penicillin-resistant staphylococci and gram-negative bacteria with a known resistance to ordinary antibiotics.	1975	1113913
sex-related incidence in proteus infection of the urinary tract in childhood.	over a 2-year period 80% of children found to have significant proteus bacteriuria were boys. the organism was isolated from the prepuce in 30% of normal boys and 32% of those with balanitis. proteus urinary tract infection should be carefully confirmed and proved cases thoroughly investigated.	1975	1108808
[study of the transferable factors of antibiotic resistance in the enterobacteria isolated at the regional hospital center of nantes].	characteristics of transferable resistance of 72 entero-bacteriaceae resistant to one more antibiotic, isolated from february to september 1972, showed that this was common. multi-resistant strains permit one to obtain transfer of various characteristics either alone or together in 76 per cent of cases, whereas strains with less than three characteristics, transferred in only 9 per cent of cases. the transfers were uncommon in the case of proteus compared with other enterobacteriaceae and, in pa ...	1975	1108714
evaluation of amniotic fluid for aerobic and anaerobic bacteria.	studies of 117 pregnant women, 83 at term, were instituted to determine the bacteriologic state of amniotic fluid, utilizing both standard aerobic and anaerobic technics. a high association of postpartum infection was found in women who had long periods of premature reptured membranes and many vaginal examinations. significant organisms including anaerobes, were isolated in many of these instances. based on the findings of these studies, it is recommended that amniocentesis for aerobic and anaer ...	1976	1108641
[amino acid decarboxylases in bacteria of the genus proteus].		1975	1108516
[influence of the storage of slaughtered birds at low temperature on the viability of some microorganisms].	studied was the effect of the period of storing slaughtered broiler birds at low temperatures on the activity of pathogenic staphylococci, enteropathogenic escherichia coli organisms and pathogenic species of the proteus genus. it was found that freezing at minus 34 degrees c and storage of the slaughtered birds at minus 18 degrees c contribute to the considerable decrease in the bacterial count of the investigated test organisms, this being observed to a highest extent during the first fifteen ...	1975	1108407
twenty-eight-day courses of antibiotics for urinary tract infection.	a prospective double-blind study was performed in which 30 adult patients with recurrent urinary tract infections due to escherichia coli, and proteus mirabilis organisms were treated by twenty-eight-day courses of either ampicillin, 500 mg. four times a day, or trimethoprim-sulphamethoxazole, 2 tablets twice a day. in terms of freedom from infection fifty-six days after the cessation of treatment in both complicated and uncomplicated infections, trimethoprim-sulphamethoxazole yielded results su ...	1976	1108350
[bactericidal properties of volatile fractions of garlic phytoncids].	changes in the bactericidal effect of volatile fractions of garlic were examined during storage. b. coli communis, proteus vulgaris and staph. pyogenes aureus 209 were used as test-organisms. bactericidal properties of volatile fractions of garlic phytoncids were most pronounced prior to storage. during storage they reduced. at higher temperature (16 degrees) the decline in the bactericidal properties was much more significant than at low temperature of storage (2 degrees).	1975	1107992
[study of the antibacterial activity of ftorlon threads].	the study concerned antibacterial properties of the ftorlon threads obtained from acetone or dimethylformamide polymer solution containing antibacterial derivatives of 5-nitrofuran added which were known to be active against the germs most commonly causing the postoperative infection including staphylococci, escherichia coli, pseudomonas aeruginosa, proteus vulgaris and candida type fungi. basing of the laboratory test following the method of infected medium and suspension method it was found th ...	1975	1107978
[ulcerative enterocolitis in infants].		1975	1107951
infected total hip replacement after dental procedures.	three cases are reported in which there was a worrisome association between dental work and an infected total hip replacement. the patients had long asymptomatic intervals subsequent to implantation of prosthetic hip joints. after dental procedures, infections became apparent in these hips. such infections carry an enormous and crippling morbidity. the potential complications of transient bacteremia in the patient with a cardiac valvular prosthesis are appreciated and the importance of prophylac ...	1976	1107928
dna repair in proteus mirabilis. iii.survival, dimer excision, and uv reactivation in comparison with escherichia coli k12.	measurements of uv sensitivity of wildtype cells (wt) and uv senistive mutants of e. coli and p. mirabilis suggest that the increased sensitivity of p. mirabilis (wt) is due either to incomplete repair of dna lesions or to additive lethality probably as a result of uv induction of defective phage(s) present in p. mitabilis (taubeneck, 1967). direct estimates of the rate of pyrimidine dimer excision and a comparison of the uv reactivation capacity of e. coli and p. mirabilis for the temperate pha ...	1975	1107808
properties of plasmids produced by recombination between r factors of groups j and fii.	recombinant plasmids have been produced both by transduction of genetic material from fiir factors into proteus mirabilis strains carrying plasmids of group j and by insertion of a transposon conferring streptomycin and trimethoprim resistances into a j group r factor. the transposon-carrying derivative and one of the transductants were shown to be members of group j whereas another transductant was shown to be compatible with members of this group. this recombinant plasmid was able to eliminate ...	1975	1107800
[studies of correlative relations between virulence and other biological properties of proteus].		1975	1107766
the phylogenetic status of pasteurella pestis.	yersinia pestis has been characterized in terms of fingerprints of digests (pancreatic and/or t1 ribonuclease) of its 16s and 5s ribosomal rnas. these show clearly that y. pestis is a member of the enterobacteriaceae and suggest that within the family it is most closely related to serratia and/or proteus.	1975	1107566
control of microflora in stallion semen with a semen extender.	sterile equipment was used to collect ten ejaculates from each of ten normal stallions and quantitative and qualitative bacterial counts were made within 15 min after collection. the mean bacterial population in undiluted semen was found to be 573,000 +/- 374,000 organisms/ml. the bacterial content of semen diluted with two parts sterile physiological saline, or with two parts of a cream-gelatin extender, was measured within 15 min after collection and again after 2 hr at 25 degrees c. the numbe ...	1975	1107540
marked suppression of thyroid function in rats with gram-negative septicemia.	gram-negative septicemia was induced in rats by two daily injections of fecal mixture into the thigh, after which the thyroid function was markedly suppressed for 2 days. iodine metabolism was studied by organ radioassay and by imaging with a multiwire proportional chamber (mwpc) at various time intervals after intravenous injection of 125i. plasma t3, t4, and tsh, measured by radioimmunoassays, were suppressed, as were the t3-resin uptakes. fractional blood supply to the thyroid glands of the i ...	1976	1107490
the elimination of urease activity in streptococcus faecium as evidence for plasmid-coded urease.	a strain of streptococcus faecium from the sheep rumen showed spontaneous loss of urease activity when subcultured at the normal rumen temperature of 38 degrees c, although in mixed cultures in vivo or in vitro loss of urease was not apparent. the rate of loss of urease in pure cultures was increased at incubation temperatures above 38 degrees c, but loss was never complete. however, at temperatures below 38 degrees c loss was greater, and at 22 or 18 degrees c the urease was completely eliminat ...	1976	1107485
physical properties of l-asparaginase from serratia marcescens.	purified l-asparaginase from serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. a subunit molecular weight of 31,500 +/- 1,500 was estimated for the enzyme after treatment with sodium dodecyl sulfate and urea and electrophoresis on polyacrylamide gels; a similar value was obtained by high-speed sedimentation equilibrium in ...	1976	1107330
extrachromosomal deoxyribonucleic acid in r factor-harboring enterobacteriaceae.	extrachromosomal deoxyribonucleic acid (dna) from 24 different r factor-harboring enterobacteriaceae was isolated and characterized by analytical ultracentrifugation and electron microscopy. the r factors represented 15 different patterns of transferable drug resistance found in enterobacteria from an enclosed geographic area. all of the strains contained extrachromosomal, circular dna molecules within the range of 0.4 to 52 mum. more than one size class of circular dna molecules was observed in ...	1976	1107322
plasmid-determined beta-lactamase indistinguishable from the chromosomal beta-lactamase of escherichia coli.	a plasmid, derived from a naturally occurring strain of proteus mirabilis, conferred resistance to cephalosporins, apparently mediated by a beta-lactamase indistinguishable from that determined by the chromosomal gene of escherichia coli k-12. there was evidence for a recombination event between the wild-type plasmid and a defective f factor (fsp) in the escherichia coli k-12 culture in which it was stored.	1976	1107303
significance of anaerobic bacteria isolated from the urinary tract. ii. experimental studies.	an attempt to cause retrograde urinary tract infection with bacteroides fragilis (a strain subcultured in artificial media) failed to produce any significant renal infection in rats. intravenous inoculation with fusobacterium (a strain subcultured in artificial media) also did not cause demonstrable renal infection in rats. nor could the anaerobic organism be demonstrated in the kidneys of these animals. animals receiving bacteroides (a strain subcultured in artificial media) inoculated directly ...	1976	1107257
[peritonitis in infants, small children and childhood].		1975	1106963
[synergism of colistin and sulfonamide in proteus species (author's transl)].	in agar diffusion testing of proteus mirabilis strains sensitive to sulfonamide a synergistic effect of colistine (c) and sulfonamide (s) was demonstrated. by quantitative evaluation these results were confirmed in 100 strains of proteus mirabilis and 100 strains of indol-positive proteus spp. using broth dilution method. combining 1 part c with 10 parts s the mean increase in sulfonamide sensitivity was enhanced fourfold. increasing the inoculum sensitivity of proteus against the combination of ...	1975	1106440
reduction of tetrathionate, trithionate and thiosulphate, and oxidation of sulphide in proteus mirabilis.	the reductase catalyzing the reduction of tetrathionate and thiosulphate in proteus mirabilis is also concerned with the reduction of trithionate and the oxidation of sulphide. tetrathionate is reduced to thiosulphate, thiosulphate to sulphite and sulphide, and trithionate is reduced to thiosulphate plus sulphite. the oxidation of sulphide in cell-free extracts proceeds most likely to polysulphanes or to elemental sulphur, depending on the conditions. the kinetics of the reduction of tetrathiona ...	1975	1106343
[some problems of nomenclature and taxonomy of the enterobacteriaceae].		1975	1106093
characterization of a stable spheroplast type l-form of proteus mirabilis d 52 as cell envelope mutant. i. isolation, growth characteristics, biochemical activities, and sensitivity to bacteriophages.	a stable spheroplast type l-form could be isolated by transferring 627 single colonies and 195 agar blocks with several colonies of unstable l-forms of proteus mirabilis d 52 on agar media without supplements of penicillin. the l-form grows well on complex and synthetic agar media, however, it failed to grow in any of the liquid media which have been proved. with one exception (formation of acid from maltose) the l-form shows the same bioche mical activities like the parent rod-shaped bacterium. ...	1975	1105990
transformation of lymphocytes in patients with certain bacterial infections.	the state of cell-mediated immunity was measured by the morphological method of lymphocyte transformation with and without pha, in a group of 56 patients with bacterial infections and in a group of healthy controls. the patients were divided into three subgroups according to the aetiology: 1. patients with gram-positive infections, 2. patients with gram-negative infections, and 3. mixed infections. the transformation values with pha were significantly (t-test) higher in the gram-positive subgrou ...	1975	1105129
r-factors in urinary tract infection.		1975	1104976
morbidity, age and sex distribution, recurrences and renal scarring in symptomatic urinary tract infection in childhood.		1975	1104973
[sensitivity of bacteria inducing urinary infections to 3 drugs (amoxicillin, ampicillin and cephalexin) and clinical efficacy of amoxicillin (author's transl)].	mic of amoxicillin (ampc), ampicillin (abpc) and cephalexin (cex) against bacteria detected from the patients suffering from urinary infections was determined and the clinical efficacy of ampc was examined. as the result of determining mic of ampc against the detected escherichia coli, 85.4% showed the value of less than 6.25 mcg/ml. ampc and abpc seem to be well correlated with each other regarding mic, and ampc showed 1 approximately to 2 stage lower concentration than cex. in connection to cl ...	1975	1104914
[in vitro examination on antibacterial activities of broad-spectrum antibiotics against gram-negative clinical isolates (author's transl)].	in vitro studies were undertaken on clinically isolated strains of various bacteria to examine the antibacterial activity of ampicillin (ab-pc), carbenicillin (cb-pc), sulbenicillin (sb-pc) cephalothin (cet), cefazolin (cez) and gentamicin (gm). fifty three % of escherichia coli strains 53.3% of citrobacter sp., 80% of proteus mirabilis, 42.9% of morganella and 80% of bacteroides sp. were susceptible (mic being not higher than 12.5 mug/ml) to ab-pc. the eight other species were not so susceptibl ...	1975	1104913
[studies on the disc method for the determination of bacterial sensitivity to sulfobenzylpenicillin (author's transl)].	the mic''s of sulfobenzylpenicillin (sb-pc) were determined by the two-fold serial agar-dilution method for 108 bacterial strains of 21 species. the diameters of inhibition zones of these bacterial strains by the 30 mug and 200 mug sb-pc discs were also measured. the relation between the mic and the diameter of the inhibition zone was found to be expressed as a primary regression line in all cases of the conventional method (cultured for about 16 hours), delayed assay method (cultured for about ...	1975	1104912
specialized transduction of a leucine marker by proteus mirabilis phage 5006m.		1975	1104770
selective medium for growth of proteus.	a medium containing heart infusion agar supplemented with bile salts, lithium chloride, sodium thiosulfate, and sodium citrate was developed for the selective growth of proteus.	1975	1104657
classification of cephalosporins by their antibacterial activity and pharmacokinetic properties.		1975	1104556
attempt to enhance resistance to infection in 6-mercaptopurine treated mice by lysozyme administration and immunization with escherichia coli o86 antigen.	long-term immunosuppressive therapy of mice with 6-mercaptopurine (6-mp) for 2 and/or 3 weeks results in partial lethality, decrease of total leukocyte count, of serum lysozyme level and in bacteremia. the adverse effect of 6-mp treatment could not be prevented by lysozyme administration; immunization with escherichia coli o86 antigen further increased the lethality of 6-mp in mice. the results stress the potential danger of immunization with bacterial antigens during immunosuppressive therapy.	1975	1104427
further investigations of proteus mirabilis lipopolysaccharides.		1975	1104102
septic shock.	the problems associated with septic shock are highlighted, including those which arose during a 3-year prospective study. mortality in the first year of the study was 71 per cent, falling to 38 per cent in the third year. the reasons for this fall are detailed. the pattern of survival times changed over the 3 years, giving rise to the longer term complications such as stress bleeding, multiple organ failure and superadded infection.	1975	1104040
matrix calculi.	matrix calculi are an uncommon form of urinary tract concretion. they must be considered in the differential diagnosis of a radiolucent mass within the renal collecting system or ureter. the clinical and radiographic features of three cases are presented and the literature of matrix calculi is reviewed.	1975	1104035
enumeration of high numbers of bacteria using hydrophobic grid-membrane filters.	printing a wax grid on a conventional membrane filter yields a device functioning as a most probable number apparatus (mpn), used at a single dilution but with a very large number of growth compartments (e.g., 3,650). by restraining the lateral spread and confluence of colonies, the hydrophobic grid-membrane filter (hgmf) allows growth- or colony-forming units (gu) to be resolved at levels far above those which produce an uncountable lawn on a conventional membrane filter. it also eliminates the ...	1975	1103728
resistance to beta-lactam antibiotics proteus strains.	a total of 218 proteus strains isolated from clinical sources were tested for their susceptibility to three penicillins and two cephalosporins. the ability to beta-lactamase production was examined in 36 of these strains. proteus mirabilis strains were generally more susceptible to cephalosporins than to penicillins, whereas indole-positive protei were almost uniformely resistant to cephalosporins as well as to ampicillin and benzylpenicillin but susceptible to carbenicillin. fairly good correla ...	1975	1103580
bacterial flora in relation to cataract extraction. iii. postoperative flora.	the conjunctival flora of 499 patients operated for cataract was studied on the 4th and 7th postoperative days and compared with the flora examined previously on admission to the hospital and at the time of surgery (fahmy et al. 1975b, c). antibiotics had been administered approximately 18 hours before operation, at the conclusion of surgery, and then on the 4th postoperative day after the cultures had been taken. after a significant fall in incidence, including all kinds of bacteria at the time ...	1975	1103562
[modification of problem pathogens in chronically infected bladders through irrigation and instillation agents].		1975	1103518
[dependence of various antigen formation of proteus mirabilis strain 1095/67 growing on minimal nutrient media].	proteus mirabilis strain 1095/67 has been cultivated in surface culture on different minimal media, which were composed either of single amino acids or combinations of several amino acids without or with stepwise addition of other c-sources up to the complete medium. we found a direct dependence of the formation of the various antigens (moving to the cathode, to the anode, or not moving) on the composition of the medium. certain amino acids (ala, glu, asp. ser) elicit only parts of the antigenic ...	1975	1103489
reduction of azo food dyes in cultures of proteus vulgaris.	1. rates of reduction of a number of azo food dyes were measured in anaerobic cultures of proteus vulgaris. the rates of colour loss were found to be zero order under conditions in which the concentration of viable cells remained constant. 2. a significant increase in the rate of reduction followed the onset of cell mortality. 3. the zero-order rates correlate with the redox potentials of the dyes. a mechanism consistent with these observations involes an extracellular non-enzymic reducing agent ...	1975	1103488
bacteremia arising from the urinary tract.		1975	1103413
therapeutic guidelines in the management of urinary tract infections and pyelonephritis.		1975	1103409
susceptibility of a hybrid plasmid to excision of genetic material.	a 5 megadalton segment of dna carrying a gene for kanamycin resistance from r447 b (a plasmid of group n of molecular weight 33 megadaltons) has been inserted into plac (a plasmid of the a--c complex of molecular weight 101 megadaltons) to produce the recombinant plasmid plac-r447 b (coetzee, 1974). the recombinant plasmid is a typical member of the a--c complex except that entry of an n group plasmid into a plac-r447 b+ recipient frequently leads to the loss of 5 megadaltons of dna (including t ...	1975	1102953
the significance of cardiac arrhythmias in the septic patient.		1975	1102867
[studies on the in vitro effects of cephalothin and gentamicin, alone and in combination, on proteus mirabilis and enterococci (author's transl)].	a combined antibiotic therapy is only useful in a few precisely defined clinical pictures where testing of the chemotherapeutics administered is required to determine their characteristics of action (antagonism, indifference, synergism) on the isolated organism. for the initial therapy of critical acute infections, simultaneous administration of cephalothin and gentamicin proved to be valuable. in the present study, the efficiency of these chemotherapeutics alone and in combination was investiga ...	1975	1102455
identification of ribitol phosphate as a constituent of the lipopolysaccharide from proteus mirabilis, strain d52.	a polyol was released from the lipopolysaccharide of proteus mirabilis, strain d52, during alkaline hydrolysis and its phosphate ester was isolated after acid hydrolysis. this polyol has been identified as ribitol by comparison of the free polyol, its phosphate ester and its anhydro derivative formed after acid treatment with authentic xylitol, d- and l-arabitol, ribitol and their corresponding derivatives on paper and gas-liquid chromatography.	1975	1102310
the isolation of two different lipopolysaccharide fractions from various proteus mirabilis strains.	four distinct proteus mirabilis strains were extracted by the phenol/water procedure. after ultracentrifugation of the dialyzed water phase, the pelleted lipopolysaccharide was purified and analyzed. the sugar composition of this lipopolysaccharide fraction i was similar for all four strains, containing only small amounts of strain-specific constituents. a second lipopolysaccharide fraction was isolated from the supernatant above (termed l1 fraction) after removal of nucleic acids. deae-cellulos ...	1975	1102309
potent combinations of beta-lactam antibiotics using the beta-lactamase inhibition principle.	several penicillins known to be stable to enterobacterial beta-lactamases were tested in combination with beta-lactamase-sensitive penicillins and cephalosporins in a turbidimetric system. nafcillin was found to be the best beta-lactamase inhibitor amongst agents presently available commercially, but the related, new semi-synthetic penicillin brl 1437 (2-isopropoxy-1-naphthylpenicillin) was consistently found to be superior. using 103 ampicillin-resistant coliform bacilli and antibiotic levels a ...	1975	1102266
effect of surfactants on antibiotic resistance.	the effectiveness of surfactants as potentiators of antibiotic activity on several resistant strains of bacteria, selected from clinical sources and laboratory collections, was studied using a tube dilution assay. bacterial strains included members of the enterobacteriaceae and staphylococci. cetyltrimethylammonium bromide (ctab), tween 80 (tw80), a mixture of n-alkyldimethyl betaines (l14), and alpha-(2,4,5-trichlorophenoxy) propionic acid (tcp) were tested in combination with pencillin g (peng ...	1975	1101823
[swarming phenomenon of an aeromonas spec (author's transl)].	a genuine swarming phenomenon, such as has previously been known to occur in proteus, bacillus and clostridium species only, was observed in an aeromonas species. fig. 1 shows the terraced swarming zones of the aeromonas species on nutrient agar. the swarming rate, expressed as the growth of the swarming zone per time unit, was measured to be 70-120 mum/min on blood agar at 30 degrees c. the swarming could be inhibited by incubation at 37 degrees c (table 2), by low saline concentrations (table ...	1975	1101579
bacteremia in a general hospital. a prospective study of 102 consecutive cases.	a prospective clinical-bacteriological study of 102 consecutive cases of confirmed bacteremia at a copenhagen city general hospital was carried out during 5 months of 1973 with special concern given to focus of infection and acquisition of microorganisms. valid positive cultures were obtained from 7.2 patients per 1000 admissions. 50 of the 102 bacteremias were by all probability acquired in the hospital, mainly due to transurethral manipulations or intravenous lines. pneumonia and hepatobiliary ...	1975	1101372
septic arthritis in children.	when septic arthritis involves the hip, the prognosis is much worse than with any other joint. approximately 50 per cent of the results were unsatisfactory with hip involvement compared to 12 per cent unsatisfactory results with involvement of the other joints. the erythrocyte sedimentation rate is a much more helpful diagnostic test than the leukocyte count or differential cell count. the most important prognostic factors are the duration of symptoms prior to treatment, a penicillinase-producin ...	1975	1101133
extensive segments of the escherichia coli k12 chromosome in proteus mirabilis diploids.	various escherichia coli k12 hfr donors transfer at low frequency portions of the e. coli genome to proteus mirabilis. by remating such proteus hybrids with the same or a different e. coli hfr strain, other genetic characters could be added to yield diploid proteus hybrids which contained more than 30 percent of the e. coli genome. the extent of the e. coli genetic material in these unstable proteus diploid hybrids included segments with the following selected markers: gal, lac, ara, mel, mtl, a ...	1975	1101034
transduction of a proteus vulgaris strain by a proteus mirabilis bacteriophage.	only proteus vulgaris strain pv127 out of many p. vulgaris, p. morganii and providence strains was transduced to kanamycin resistance by high-frequency transducing variants, 5006mhftk and 5006mhftak, of phage 5006m, a general transducing phage for p. mirabilis strain pm5006. the phages adsorbed poorly to strain pv127 and did not form plaques. the transduction frequency of pv127 by these phages was 5 x 10(-8)/p.f.u. adsorbed. phage 5006m increased the transduction frequencies. abortive transducta ...	1975	1100773
rapid identification of swarming proteus using the pathotec ornithine decarboxylase test strip.	the pathotec ornithine decarboxylase test strip was evaluated for use in identification of swarming proteus. all 181 strains tested were accurately identified within 4 h.	1975	1100667
amino-acid composition of the covalent rigid-layer lipoprotein in cell walls of proteus mirabilis.	cell walls of proteus mirabilis in the stationary phase of growth contain a lipoprotein in covalent linkage to peptidoglycan and probably also in free form in the outer membrane. the protein moiety of this lipoprotein is composed of about 50 amino acids and has an approximate molecular weight of 5500. the proteus lipoprotein has glycine and phenylalanine as specific components which are not present in lipoproteins of other enteric bacteria. treatment of the peptidoglycan-lipoprotein complex of p ...	1975	1100405
cephalexin in the management of bacteriuria.	this report evaluates the effectiveness of cephalexin in the treatment of bacteriuria in 93 children. cephalexin therapy eradicated sensitive organisms in 97 per cent of the cases regardless of recurrence, structural abnormality, or status of renal function. nevertheless, recurrences with a new, resistant organism occurred significantly in certain patients, expecially those with major anatomic abnormalities, during a six-week follow-up period. the incidence of drug reactions was low.	1975	1100295
effective selection of proteus mirabilis clones producing mirabilicin d-52.	from a defective-lysogenic proteus mirabilis strain we isolated several clones differing in the pattern of their growth on agar plates. using electron microscopy we have shown some of the selected clones to be efficient in producing mirabilicin d-52 after uv induction, while other clones produced defective mirabilicin polysheaths and polycores. clones producing polysheaths and polycores can be detected electron microscopically only, since these defective particles are biologically inactive.	1975	1100207
editorial: a clinical classification of urinary tract infections based upon origin.		1975	1099658
evaluation of the usefulness of nitroblue tetrazolium reduction test (nbt test) for detection of bacterial infection in cancer patients.	false positivity of the nbt test in neoplastic diseases described by some authose may be a limitation in the use of the test for infection screening in cancer patients. for explaining this problem studies were performed in a group of 30 patients with various untreated malignancies without infection and in 20 of these with bacteriologically confirmed infections. the obtained results were compared with those in groups of normal persons without and with infection. slightly modified park method for ...	1975	1099471
[phage typing of proteus mirabilis].		1975	1099367
arginine gene cluster of serratia marcescens.	biochemical and genetic studies on the arginine-requiring auxotrophs derived from a serratia marcescens strain were carried out. the arg mutants were classified into seven biochemical groups based on their growth response to five precursors of arginine biosynthesis and enzyme deficiency. reciprocal transduction tests among these arg mutants divided them into three linkage groups, and the fine mapping in each of the groups by two- or three-point crosses revealed the following arrangement of loci. ...	1975	1099287
synthesis and antibacterial evaluation of 1,2,3,4-tetrahydro-4-oxo-1,8-naphthyridine-3-carboxylic acid esters, carbonitriles, and carboxamides.	a series of 1,2,3,4-tetrahydro-4-oxo-1,8-naphthyridine-3-carboxylic acid esters, carbonitriles, and carboxamides (2a-k) was synthesized and initially evaluated (dose range 50-400 mg/kg) in mice infected with escherichia coli. only two derivatives, the ethyl and butyl esters of 1-ethyl-1,2-dihydro-4-hydroxy-7-methyl-1,8-naphthyridine-3-carboxylic acid, protected the animals against e. coli and several other gram-negative bacterial pathogenic infections. a pro-drug type of mechanism appears to be ...	1975	1099201
crystallographic studies on l-asparaginase from proteus vulgaris. ii. symmetry and location of the tetrameric molecule.	analyses of the x-ray diffraction intensity data by the patterson synthesis and rotation function techniques show that the true space group of the monoclinic crystals of l-asparaginase (l-asparagine amidohydrolase, ec 3.5.1.1) from proteus vulgaris is p21, that the molecular centers lie at x = 0.054, y = 0, z = 0.256, and its symmetry related positions, and that the tetramer molecules possess three approximate, mutually perpendicular 2-fold rotational symmetries, the axes of which run along the ...	1975	1099088
denaturation mapping of r factor deoxyribonucleic acid.	the r factor nr1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (rtf-tc) and the r-determinants component which harbors the other drug resistance genes. using partial denaturation mapping it is possible to distinguish the rtf-tc region from the r-determinants region of the composite r factor nr1 dna which has a contour length of 37 mum and a density of 1.712 g/ml. the r-determinants region was a relatively undenatured 8.5-mum segment of t ...	1975	1099070
transition of r factor nr1 in proteus mirabilis: molecular structure and replication of nr1 deoxyribonucleic acid.	the structure of r factor nr1 dna in proteus mirabilis has been studied by using the techniques of cscl density gradient centrifugation, sedimentation in neutral and alkaline sucrose gradients, and electron microscopy. it has been shown that the nontransitioned form of nr1 dna isolated from p. mirabilis cultured in drug-free medium is a37-mum circular deoxyribonucleic acid (dna) with a density of 1.712 g/ml in a neutral cscl gradient. this circular molecule is a composite structure consisting of ...	1975	1099069
determination of ammonia inhibition in fabric impregnated with antimicrobials.	a new method is described for determining the presence or absence of ammonia produced by urealytic microorganisms, using fabric which has been treated with an antimicrobial. circular disks of fabric treated with various concentrations of an antimicrobial are placed in the bottoms of snap-on lid petri dishes. nutrient medium containing urea, an indicator, and a dilution of an overnight culture of proteus mirabilis is applied to the fabric disks. the lids are snapped on and the dishes are incubate ...	1975	1099065
the kinetic of degradation of chondroitin of sulphates and hyaluronic acid by chondroitinase form proteus vulgaris.	km and vmax. were determined for the degradation by chondroitinase of chondroitin 4-sulphate, 4-sulphate-proteoglycna, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid. degradation of chondroitin 4-sulphate was inhibited by hyaluronic acid but not by keratan sulphate. the results are discussed with regard to the use to the use of chondroitinase as a sleective reagent for the degradation of tissue glycosaminoglycans.	1975	1098655