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[clinical strain of proteus mirabilis able to produce a plasmid mediated ceftazidimase.]resistance to third generation cephalosporins due to the acquisition and expression of extended spectrum beta-lactamases (esbls) enzymes among gram negative bacteria continue to pose a challenge to infection management worldwide. the aim of this study was to determine the implication of the enzymatic mechanism and to describe the properties of esbls from a clinical strain of proteus mirabilis nc4150 isolated in the intensive care unit at the military hospital in tunisia during the period 2004. t ...200717574349
some observations on the diffusion of antimicrobial agents through the retention balloons of foley catheters.we examined the ability of antimicrobial agents to diffuse through the retention balloons of urinary catheters and inhibit their encrustation by proteus mirabilis.200717574612
kasabach-merritt syndrome caused by giant hemangiomas of the spleen in patients with proteus syndrome.proteus syndrome is a rare hamartomous disorder with highly variable manifestations of disproportionate overgrowth of body parts, epidermal nevi and vascular malformations. we describe two patients who had typical clinical and imaging proteus syndrome features. one of most characteristic findings in these two patients was that they had giant hemangiomas of the spleen that caused kasabach-merritt syndrome, demonstrating clinical bleeding and disseminated intravascular coagulation laboratory alter ...200717581327
[encephalocraniocutaneous lipomatosis (haberland syndrome) with bilateral ocular damage].encephalocraniocutaneous lipomatosis, or haberland syndrome, is an unusual form of neurocutaneous syndrome. clinically, this syndrome is characterized by unilateral lipomatous hamartomas on the scalp and face, ocular damage and ipsilateral malformations of the central nervous system. first described by haberland and perou in 1970, only about 40 cases have been reported to date. the differential diagnoses with other neurocutaneous mosaicisms such as delleman, goltz, goldenhar or proteus syndrome ...200717583626
[rocky mountain spotted fever in children: clinical and epidemiological features].to report the clinical features of the rocky mountain spotted fever (rmsf) in children of southern sonora, mexico.200717585701
kohl - the traditional eyeliner: use and analysis.kohl is a traditional powder-like mixture used as an eyeliner and to treat eye disease in many middle and far east countries. we studied kohl use among 360 individuals selected at random in riyadh, saudi arabia. kohl was used by 13% of males and 26% of females, especially the elderly and illiterate. forty percent of females with children applied kohl to the eyes of their children during the first postnatal month. analysis of five commercially available traditional kohl samples showed that some p ...199317587986
use of gas liquid chromatography as an adjunct to conventional bacteriological methods in the diagnosis of anaerobic cerebral abscess.forty cases of cerebral abscesses were studied prospectively to establish the microbial agents implicated in these cases. chronic otitis media (14 patients, 35%), congenital heart disease (five patients, 12.5%),a and meningitis (five patients, 12.5%) were among the important predisposing factors. streptococcus (14 patients, 35%) was the most common causative pre-isolated, the predominant species being streptococcus milleri (11 patients, 27.5%). other organisms isolated included proteus mirabilis ...199117590760
proteus syndrome with syringohydromyelia and arachnoid cyst.introduction: proteus syndrome (ps) is a rarely seen, sporadic disorder with a wide range of abnormalities including asymmetrical overgrowth, skin lesions, dysregulated adipose tissue, and vascular malformations. brain and spinal malformations are extremely rare; syringohydromyelia and arachnoid cyst have not been reported previously. materials and methods: we present a 5-year-old girl with ps having severe central nervous system (cns) abnormalities demonstrated by magnetic resonance imaging (mr ...200717593376
chronic granulomatous disease in pediatric patients: 25 years of experience.chronic granulomatous disease (cgd) is an uncommon primary immune deficiency (affecting 1/200,000 newborn infants) caused by a defect in phagocyte production of oxygen metabolites, and resulting in bacterial infections produced by catalase-positive microorganisms and fungal diseases that occasionally may prove fatal.200717594870
in vitro susceptibility of community-acquired urinary tract pathogens to commonly used antimicrobial agents in spain: a comparative multicenter study (2002-2004).the susceptibility patterns of 2724 uropathogens isolated in 9 spanish regions during 2002, and 3013 obtained in 2004 were determined. the antibiotics tested were fosfomycin trometamol, amoxicillin, co-amoxiclav, cefixime, cefuroxime-axetil, pipemidic, ceprofloxacin, trimethoprim plus sulphamethoxazole and nitrofurantoin. escherichia coli was the main pathogen in both studies (73% vs. 68.3%) followed by proteus mirabilis 7.2% vs. 6.4%) and klebsiella pneumoniae (5.4% vs. 5.2%). enteroccocus spp. ...200717594920
[incidence of nosocomial infections at the recovery clinic of iaşi hospital, in 2004-2005].the nosocomial infections remain a major public health problem. the descriptive study has been performed on a sample of 37541 patients hospitalized in the sanitary during 2004-2005. the aim of the study was to evaluate the declared and real incidence of nosocomial infections, to evaluate the risk factors and the etiological agents involved in their occurrence.200717595877
convenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory.although transmissible metallo-beta-lactamases (mbls) are a serious threat to beta-lactam antibiotic therapy, the clsi currently does not recommend testing methods for the detection of mbls. the aim of this study was to evaluate the capability of double-disk tests (ddts) by using disks containing a combination of the chelators 2-mercaptopropionic acid (mpa) and tris-edta (te) to detect mbls. sixteen isolates (4 acinetobacter baumannii isolates, 6 pseudomonas aeruginosa isolates, 1 serratia marce ...200717596358
structure of a new ribitol teichoic acid-like o-polysaccharide of a serologically separate proteus vulgaris strain, tg 276-1, classified into a new proteus serogroup o53.an unusual ribitol teichoic acid-like o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide from a previously non-classified proteus vulgaris strain tg 276-1. structural studies using chemical analyses and 2d (1)h and (13)c nmr spectroscopy showed that the polysaccharide is a zwitterionic polymer with a repeating unit containing 2-acetamido-4-amino-2,4,6-trideoxy-d-galactose (d-fucnac4n) and two d-ribitol phosphate (d-rib-ol-5-p) residues and having the following struc ...200717601512
new aspects of membrane dynamics of amoeba proteus contractile vacuole revealed by vital staining with fm 4-64.the contractile vacuole (cv) cycle of amoeba proteus has been studied by phase contrast and electron microscopy. however, the understanding of membrane dynamics in this cycle is still poor. in this study, we used live imaging by fluorescence microscopy to obtain new insights. we succeeded in staining the cv with a styryl dye, fm 4-64 (n-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium dibromide), and obtained the following results. (1) the cv membrane was directly s ...200717602276
actin dynamics in amoeba proteus motility.we studied the distribution of the endogenous arp2/3 complex in amoeba proteus and visualised the ratio of filamentous (f-actin) to total actin in living cells. the presented results show that in the highly motile amoeba proteus, arp2/3 complex-dependent actin polymerisation is involved in the formation of the branching network of the contractile layer, adhesive structures, and perinuclear cytoskeleton. the aggregation of the arp2/3 complex in the cortical network, with the exception of the uroi ...200717602277
synthesis and structure of silver complexes with nicotinate-type ligands having antibacterial activities against clinically isolated antibiotic resistant pathogens.the synthesis and low-temperature x-ray crystal structures of five new silver complexes, [ag(2)-mu-o,o'(2-aminonicotinium)(2)(no(3))(2)](n) (7), [ag(isonicotinamide)(2)-mu-o,o'(no(3))](2) (8), [ag(ethyl nicotinate)(2)](no(3)) (9), [ag(ethyl isonicotinate)(2)(no(3))] (10), and [ag(methyl isonicotinate)(2)(h(2)o)](no(3)) (11), are presented and fully characterized by spectral and elemental analysis. the antimicrobial activities of these complexes were screened using 12 different clinical isolates ...200717602550
in vitro activity of cefadroxil, cephalexin, cefatrizine and cefpirome in presence of essential and trace elements.evidences supporting the introduction of metallic elements in several biological processes are rapidly accumulating. likewise, many drugs possess modified toxicological and pharmacological properties when in the form of metal complexes. in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various cephalosporins, the synergistic or antagonistic behavior of cefadroxil, cephalexin, cefatrizine and cefpirome in presence of essential and ...200717604254
antimicrobial activity against gram negative bacilli from yaounde central hospital, cameroon.antimicrobial resistance among bacteria pathogens is a world-wide issue. the antimicrobial susceptibility patterns of common pathogenic bacteria are essential to guide empirical and pathogen-specific therapy; unfortunately, these data are scarse in cameroon.200617604512
[comparison of susceptibilities for fosfomycin determined by various methods in clinical isolates in 2003 -2004].antimicrobial susceptibilities for fosfomycin (fom), cephalexin, cefpodoxime, cefdinir, cefditren, ampicillin, sulbactam/ampicillin, imipenem (ipm), panipenem, meropenem (mepm), biapenem, levofloxacin (lvfx), gatifloxacin, pazufloxacin, prulifloxacin and sulfamethoxazole/trimethoprim were determined by an agar dilution method using mueller-hinton agar (mha) in escherichia coli, klebsiella spp., serratia marcescens, citrobacter spp., enterobacter spp. and proteus mirabilis, which were isolated fr ...200717612258
a two-step procedure for automatic and accurate segmentation of volumetric clsm biofilm images.this paper presents a robust two-step segmentation procedure for the study of biofilm structure. without user intervention, the procedure segments volumetric biofilm images generated by a confocal laser scanning microscopy (clsm). this automated procedure implements an anisotropic diffusion filter as a preprocessing step and a 3d extension of the otsu method for thresholding. applying the anisotropic diffusion filter to even low-contrast clsm images significantly improves the segmentation obtain ...200717618700
hyperoxaluria, hypocitraturia, hypomagnesiuria, and lack of intestinal colonization by oxalobacter formigenes in a cervical spinal cord injury patient with suprapubic cystostomy, short bowel, and nephrolithiasis.although urolithiasis is common in spinal cord injury patients, it is presumed that the predisposing factors for urinary stones in spinal cord injury patients are immobilization-induced hypercalciuria in the initial period after spinal injury and, in later stages, urine infection by urease-producing micro-organisms, e.g., proteus sp., which cause struvite stones. we describe a patient who sustained c-7 complete tetraplegia in a road traffic accident in 1970, when he was 16 years old. left ureter ...200617619709
investigation of essential oil extracts from four native jamaican species of bursera for antibacterial activity.bacterial infection with organisms resistant to antibiotics have increased during the last few decades worldwide. because of this increase, the authors decided to subject the essential oils from the stem, leaves and fruits of the four native jamaica species of bursera to microbial studies.200717621840
some aspects of epidemiology of filth flies: musca domestica, musca domestica vicina, drosophilia melanogaster and associated bacteria pathogens in ekpoma, nigeria.between january 2005 and december 2005, including the wet and dry seasons, 1,358 filth flies (musca domestica, 737 [54.3%]; m. domestica vicina 556 [41%]; and drosophilia melanogaster 65 [5%]) were caught by quadrant samplings using nets twice each month (early and later) in each of the various sites with varying sanitary conditions in ekpoma, nigeria. these sites were markets, hospitals, houses/human habitations, pit latrines, stalls of local food vendors, abattoirs/slaughterhouses, and dairy f ...200717627427
[the new mucosal adjuvant cry1ac increase the capacity of mononuclear cells to stop bacterial growth]. 200717633805
fimbriae of uropathogenic proteus mirabilis.proteus mirabilis is a common causative agent of cystitis and pyelonephritis in patients with urinary catheters or structural abnormalities of the urinary tract. several types of fimbriae, which are potentially involved in adhesion to the uroepithelium, can be expressed simultaneously by p. mirabilis: mannose-resistant/proteus-like (mr/p) fimbriae, p. mirabilis fimbriae (pmf), uroepithelial cell adhesin (uca), renamed by some authors nonagglutinating fimbriae (naf), and ambient-temperature fimbr ...200717640292
bacteriology of diabetic foot lesions.clinical grading and bacteriological study of 107 patients with diabetic foot lesions revealed polymicrobial aetiology in 69 (64.4%) and single aetiology in 21 (19.6%). among 107 patients 62 had ulcer. of these 31 had mixed aerobes. twenty six patients with cellulitis and 12 with gangrene had more than 5 types of aerobes and anaerobes such as e. coli, klebsiella spp., pseudomonas spp., proteus spp., enterobactor spp., enterococci spp., clostridium perfringens, bacteroides spp., prevotella spp. a ...200417642727
images in emergency medicine. infected urachal cyst. 200717643855
occurrence of antimicrobial resistance in bacteria from diagnostic samples from dogs.to study the occurrence of antimicrobial resistance among common bacterial pathogens from dogs and relate resistance patterns to data on consumption of antimicrobials.200717644533
suprapubic cystotomy in a stallion.a five-year-old thoroughbred stallion had been showing urinary incontinence with staining of the hind legs for some time, and was in poor physical condition. on rectal examination a large calculus was palpated in the urinary bladder. urine culture revealed a proteus infection. the calculus, weighing 420 gm., was removed by laparo-cystotomy, using a left paramedian approach well anterior to the external inguinal ring. anaesthesia was maintained with halothane during the operation. aftercare inclu ...196417649501
evaluation of colistin in the treatment of respiratory infections in dogs.thirty cases of respiratory infections in adult dogs were treated with colistimethate sodium. e. coli, pseudomonas aeruginosa, klebsiella pneumoniae, paracolobactrum sp., and aerobacter aerogenes responded to therapy, whereas proteus sp., streptococcus faecalis, streptococcus pyogenes, and staphylococcus aureus were resistant to treatment.196417649529
[proteus syndrome with a giant hemangiomas in the spleen associated with chronic dic--two case report and literature review].to investigate the clinical manifestations, pathologic features and laboratory findings in two proteus syndrome patients with giant hemangiomas in the spleen and chronic dic.200717649705
use of hp selective medium to detect helicobacter pylori associated with other enteric bacteria in seawater and marine molluscs.this project investigated the utility of hp selective medium to isolate h. pylori cells from seawater and from marine molluscs.200717651221
unilateral generalized hypertrichosis in proteus syndrome.a young lady was found to have hemihypertrophy, scoliosis, subcutaneous swellings, patchy dermal hypoplasia, plantar hyperplasia and macrodactyly. these suggested a diagnosis of proteus syndrome. she was also having unilateral generalized hypertrichosis. the association of proteus syndrome and unilateral generalized hypertrichosis has not been reported in the literature.200217656984
antibiotic resistance pattern in uropathogens.uropathogenic strains from inpatient and outpatient departments were studied from april 1997 to march 1999 for their susceptibility profiles. the various isolates were escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, acinetobacter baumanii and enterococcus faecalis. antibiotic susceptibility pattern of these isolates revealed that for outpatients, first generation cephalosporins, nitrofurantoin, norfloxacin/ciprofloxacin were effective for treatment of urinary ...200217657041
prevalence of hospital-acquired infection in a moroccan university hospital.infection control and hospital-acquired infection (hai) prevalence reports from developing countries are often not well established because of the lack of staff and resources. the aim of this study was to estimate the prevalence of hai in a moroccan hospital as well as to identify its predisposing factors.200717660013
haemagglutination, haemolysin production and serum resistance of proteus and related species isolated from clinical sources.a total of 148 strains of proteus and related species comprising of proteus mirabilis (116), proteus vulgaris (24), providentia rettgeri (4), providentia alcalifaciens (2), providentia stuarti (1) and morganella morganii (1), isolated from various sources, were examined for haemagglutination (ha), haemolysin production (hl) and serum resistance (sr). maximum isolates were obtained from urine (47.30%) and pus (40.54%) and they were multidrug resistant. the sensitivity to ciprofloxacin was 78.38%, ...200117664798
diverse tetracycline resistant bacteria and resistance genes from coastal waters of jiaozhou bay.environmental microbiology investigation was carried out in jiaozhou bay to determine the source and distribution of tetracycline-resistant bacteria and their resistance mechanisms. at least 25 species or the equivalent molecular phylogenetic taxa in 16 genera of resistant bacteria could be identified based on 16s ribosomal deoxyribonucleic acid sequence analysis. enterobacteriaceae, pseudomonadaceae, and vibrionaceae constituted the majority of the typical resistant isolates. indigenous estuari ...200817668262
hormonal regulation of the contractile vacuole in amoeba proteus: the role of cyclic nucleotide phosphodiesterases. 200717668613
an urgent problem of aerobic gram-negative pathogen infection in complicated parapneumonic effusions or empyemas.complicated parapneumonic effusion or empyema is a troublesome disease with a high mortality. the most common involved microorganisms seem to have changed over recent decades, influenced by the introduction of new antibiotics, and the increase of immunocompromised hosts, and the elderly population. more epidemiological studies on the current bacteriology are needed to help us to empirically select adequate antibiotics.200717675765
profile of community- and hospital-acquired pneumonia cases admitted to basra general hospital, iraq.over an 18-month period 485 throat swabs and sputum samples were taken from patients admitted to basra general hospital, iraq, with a clinical diagnosis of pneumonia. most patients (94.0%) had community-acquired pneumonia; 29 (6.0%) had hospital-acquired pneumonia. patients aged < or = 15 years and males had the highest incidence of pneumonia. cases were analysed by type of pneumonia: bronchial (76.3%) versus lobar (23.7%), and primary (81.0%) versus secondary (19.0%). the most common pathogen w ...200717684843
[ecthyma gangrenosum in a patient with non-hodgkin lymphoma].ecthymagangrenosum is a rare disease of the skin that causes the localized necrosis of the skin and subcutaneous fat tissue, leading to the multiple ulcerations surrounded by local hyperaemia. the ulcerations are usually localized in groins, and perianal area. in the majority of cases ecthyma is caused by a pseudomonas aeruginosa sepsis. the disease usually appears in immunocompromized, most frequently hematological patients.200717687947
synthesis and antibacterial activity of some novel chiral fluorophoric biscyclic macrocycles.synthesis of chiral permanent fluorophoric biscyclic macrocycles incorporating anthraquinone and (s)-binol core is described. interestingly, the biscyclic macrocycle 1 exhibited remarkable antibacterial activity against most of the pathogenic bacteria in the tested concentrations as compared to the other three compounds 2, 14 and 17 as well as the test control, tetracycline. further biscyclophanes 1 and 2 exhibited permanent fluorescence sensing property even under highly acidic conditions.200717689076
comparison of real-time polymerase chain reaction and conventional polymerase chain reaction methods for the rapid identification of bacillus anthracis.bacillus anthracis spores have been shown to be one of the most effective biological weapons. for the rapid detection of b. anthracis spores, several genetic markers, including chromosomal and plasmid-based sequences, were studied with polymerase chain reaction (pcr) methods. in the present study, a method using a primer/probe set based on the pxo1-encoded pag gene for the detection of b. anthracis was tested in addition to culture. eight pathological samples (four blood-immersed cotton specimen ...200717691694
apnoea-hypopnoea and mandibular retrusion as uncommon findings associated with proteus syndrome.the aetiology of proteus syndrome (ps) is yet unclear. this disease includes partial gigantism of the hands and/or feet, nevi, hemihypertrophy due to overgrowth of long bones, subcutaneous tumours, macrocephaly, cranial hyperostosis, and pulmonary and renal abnormalities. this case report is about a 17-year-old boy with two uncommon findings associated with ps: apnoea-hypopnoea and mandibular retrusion. a multidisciplinary team was important to provide professional care for this patient. dentist ...200717699709
production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses.twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ...200717701035
parenting children with proteus syndrome: experiences with, and adaptation to, courtesy stigma.courtesy stigma refers to the stigmatization an unaffected person experiences due to his or her relationship with a person who bears a stigma. parents of children with genetic conditions are particularly vulnerable to courtesy stigma, but little research has been done to explore this phenomenon. the purpose of this study was to investigate the courtesy stigma experiences of parents of children with proteus syndrome (ps) and related overgrowth conditions. thematic analysis of transcripts from 31 ...200717702022
the structure of apo tryptophanase from escherichia coli reveals a wide-open conformation.the crystal structure of apo tryptophanase from escherichia coli (space group f222, unit-cell parameters a = 118.4, b = 120.1, c = 171.2 a) was determined at 1.9 a resolution using the molecular-replacement method and refined to an r factor of 20.3% (r(free) = 23.2%). the structure revealed a significant shift in the relative orientation of the domains compared with both the holo form of proteus vulgaris tryptophanase and with another crystal structure of apo e. coli tryptophanase, reflecting th ...200717704565
[intracranial hypertension in proteus syndrome].proteus syndrome, described for the first time in 1979, is a sporadic congenital poly-malformation syndrome named for its highly variable manifestations. we report the case of a 36-year-old male patient with several malformations including skull hyperostosis and huge frontal sinus hypertrophy compressing the brain. he complained of increasing headache for 5 years. cerebrospinal fluid pressure monitoring revealed severe hypertension. the patient underwent frontoparietal craniectomy, which allowed ...200717706728
[selective action of broad-spectrum antibiotics on intestinal flora in sepsis in rats].to investigate the selective effect of broad-spectrum antibiotics on the intestinal flora in sepsis in rats.200717708836
[first report of parasitism of the trichogramma bruni nagaraja (hymenoptera: trichogrammatidae) in eggs of urbanus proteus (l.) (lepidoptera: hesperiidae) in snap beans (phaseolus vulgaris) ( l.)].aiming to verify the presence of eggs parasitoids of the genus trichogramma, for using in the biological control of insects pests, were collected eggs from long tailed skipper butterfly, a lepidopteran defoliator of snap beans, which presented eggs with parasitism characteristics (dark eggs). the eggs were taken to the laboratory were 44.9% of parasitism was verified. viability was 96.3%, with an average of 6.6 individuals per egg and a sexual ratio of 0.93. male specimens were identified as tri ...200717710338
aggregative adherence of uropathogenic proteus mirabilis to cultured epithelial cells.proteus mirabilis is an important cause of urinary tract infection (uti) in patients with complicated urinary tracts. thirty-five strains of p. mirabilis isolated from uti were examined for the adherence capacity to epithelial cells. all isolates displayed the aggregative adherence (aa) to hep-2 cells, a phenotype similarly presented in llc-mk(2) cells. biofilm formation on polystyrene was also observed in all strains. the mannose-resistant proteus-like fimbriae (mr/p), type i fimbriae and aaf/i ...200717714491
antimicrobial resistance among gram-negative bacilli causing infections in intensive care unit patients in the united states between 1993 and 2004.during the 12-year period from 1993 to 2004, antimicrobial susceptibility profiles of 74,394 gram-negative bacillus isolates recovered from intensive care unit (icu) patients in united states hospitals were determined by participating hospitals and collected in a central location. mics for 12 different agents were determined using a standardized broth microdilution method. the 11 organisms most frequently isolated were pseudomonas aeruginosa (22.2%), escherichia coli (18.8%), klebsiella pneumoni ...200717715376
diversity of microflora in the gut and casts of tropical composting earthworms reared on different substrates.the diversity of fungi, bacteria, yeast, actinomycetes and protozoa were analysed in the gut and casts of eudrilus eugeniae, lampito mauritii, eisenia fetida and perionyx excavatus, both qualitatively and quantitatively as influenced by different feed substrates like clay loam soil, cowdung and pressmud. while actinomycetes (streptomyces albus, s. somaliensis, nocardia asteroides, n. caviae and saccharomonosporia) were not digested by any of these species of worms, protozoa (amoeba proteus, a. t ...200717717992
antimicrobial activity of methanol extract of origanum majorana l. (sweet marjoram).in-vitro microbicidal activity of the methanol extract of origanum majorana l. was tested against seven fungi (fusarium solani, candida albicans, aspergillus niger, a. parasiticus, rhizopus oryzae, rhizoctonia otyzae-sativae and altemaria brassicicola) and six bacteria (bacillus subtilis, b. megaterium, escherichia coil, proteus vulgaris, pseudomonas aeruginosa and staphylococcus aureus). the methanol extract of o. majorana can be used as an effective herbal protectant against different pathogen ...200717718003
plasmid determined resistance to quinolones in clinical isolates of gram-negative bacilli.a total of 166 strains of gram-negative bacilli comprising 65 klebsiella species, 22 escherichia coli, 39 pseudomonas aeruginosa, 20 proteus species, 18 other pseudomonas species and 2 salmonella typhi, were isolated from clinical specimens in the university college hospital, ibadan. antimicrobial susceptibility testing and plasmid profiling of the strains were done. ceftazidine had the highest antibacterial activity of 83.1% compared to the fluoroquinolones followed by ciprofloxacin (78.3%), pe ...200617722809
first report of the emergence of ctx-m-type extended-spectrum beta-lactamases (esbls) as the predominant esbl isolated in a u.s. health care system.ctx-m-type extended-spectrum beta-lactamases (esbls) have become increasingly common worldwide, with the notable exception of the united states, where tem- and shv-type esbls have appeared to predominate. we have noted the emergence of esbls in our health care system (the university health system in san antonio, tx), especially in escherichia coli isolates, that preferentially hydrolyze cefotaxime rather than ceftazidime, suggesting the possibility of ctx-m-type enzymes. microbiology laboratory ...200717724160
the contractile vacuole granules in amoeba proteus. 192617739269
charles proteus steinmetz and the american institute of electrical engineers. 193417745747
the observation of mitosis in the living cell in amoeba proteus. 193417747194
proteus henricensis nov. spec. 192717752626
temperature sensitivity: a cell character determined by obligate endosymbionts in amoebas.a strain of amoeba proteus has lost its ability to survive at temperatures above 26 degrees c as a result of becoming dependent on endosymbiotic bacteria that are psychrophile-like. the observed temperature sensitivity develops in fewer than 200 host cell generations (18 months of culture) after the host cells are experimentally infected with the symbionts.197817754039
a case of proteus syndrome with lateral embryonal vein and frontal intraosseous lipoma.a patient with proteus syndrome is reported with heretofore unreported features including a frontal intraosseous lipoma and a lateral embryonal leg vein (servelle) associated with a venous malformation. these associated anomalies further broaden the phenotypic variability of proteus syndrome.200717760489
is it a proteus syndrome? 200717762684
charles proteus steinmetz. 192317770636
action of hydrogen sulfide on the protoplasm of amoeba proteus. 192917773751
amoeba proteus: studying the contractile vacuole by micropuncture.direct measurements of the freezing point depression of the protoplasm and of the fluid from the contractile vacuole of fresh-water amoebae showed that the fluid in the vacuole is distinctly hypoosmotic to the protoplasm. fourteen samples of protoplasm from amoebae, placed in a medium with a milliosmolality of 7, had an average osmolality of 101 milliosmoles with a range of 73 to 116. eleven samples of vacuolar fluid had an average osmolality of 32 milliosmoles, with a range of 24 to 38. it is s ...196317788304
concerning the species amoeligba proteus. 191617795980
a modification of the urease test for proteus. 194517799005
acquired ampc type beta-lactamases: an emerging problem in italian long-term care and rehabilitation facilities.we report the multiple detection of proteus mirabilis isolates, from 4 different long-term care and rehabilitation facilities (ltcrfs) of northern italy, resistant to expanded-spectrum cephalosporins and cephamycins and producing an acquired ampc-like beta-lactamase, named cmy-16. genotyping by pfge showed that isolates were clonally related to each other, although not identical. in all isolates the bla(cmy16) gene was not transferable by conjugation and was found to be carried on the chromosome ...200717802913
molecular epidemiology of esbetal producing p. mirabilis strains from a long-term care and rehabilitation facility in italy.we report the detection of multidrug resistant esbetal producing proteus mirabilis isolates from a long-term care and rehabilitation facility (ltcrf) in northern italy. 53% of the collected p. mirabilis strains were esbetal producers. pcr and sequencing techniques confirmed the presence of the bla(tem-92) and bla(cmy-16) resistance genes in 23/26 (88.5%) and 3/26 (11.5%) of the esbetal producers respectively. pfge showed that the tem-92 beta-lactamase producing isolates were not clonally related ...200717802927
polymerase chain reaction for the rapid diagnosis of tuberculous meningitis.polymerase chain reaction (pcr) based on the amplification of a 169 bp dna fragment specific for the mycobacterium tuberculosis complex was evaluated for the rapid diagnosis of tuberculous meningitis (tbm). a total of 105 csf specimens from clinically suspected cases of tbm were studied. clinical details of the cases and cytochemical parameters of the csf specimens were recorded. for pcr 10 csf specimens from cases other than tbm, 4 non-mycobacterial culture isolates (one strain of e coli, one s ...200717802972
microbial keratitis in a case of lamellar ichthyosis.we report a case of microbial keratitis in a patient with lamellar ichthyosis. an 11-year-old boy, a known case of lamellar ichthyosis, presented with microbial keratitis. microbiological evaluation of corneal scraping revealed a mixed infection caused by gram-negative bacilli and gram-positive cocci. he was treated with topical ciprofloxacin and fortified cefazolin eyedrops. microbial keratitis in patients with lamellar ichthyosis has a poor prognosis.200817828596
clinical epidemiology of ciprofloxacin-resistant proteus mirabilis isolated from urine samples of hospitalised patients.this study investigated the clinical characteristics of ciprofloxacin-resistant proteus mirabilis isolates from urine samples associated with nosocomial infection or colonisation, and identified the risk-factors for ciprofloxacin resistance. data for patients with ciprofloxacin-resistant p. mirabilis isolates (n=13) were compared with those for randomly selected patients with ciprofloxacin-susceptible p. mirabilis isolates (n=40) who were matched by temporal occurrence as control patients. the m ...200717850340
bacteriology of chronic suppurative otitis media--a multicenter study.bacterial predominance and antibiotic sensitivity have changed over time, making continuous and periodic surveillance necessary in guiding appropriate antibacterial therapy.200717851935
mannose-resistant proteus-like and p. mirabilis fimbriae have specific and additive roles in p. mirabilis urinary tract infections.proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (uti). it produces several types of fimbriae, including mannose-resistant proteus-like (mr/p) fimbriae and p. mirabilis fimbriae (pmf). previously, we determined that these fimbriae affect the ability of p. mirabilis to colonize the urinary tract. the objective of this study was to analyse the effect of the simultaneous lack of p. mirabilis mr/p and pmf fimbriae in uti pathogenesis. a double mutant ...200717854474
xv. the rôle of the bacillus proteus vulgaris in surgery. 190217861144
viii. the identity of proteus infection and hospital gangrene: a case of mixed infection with aerogenes capsulatus and proteus vulgaris. 190217861211
[the antibacterial activity of fluoroquinolones against gram-negative bacteria isolated from gifu prefecture (2005)].we investigated the susceptibility to 6 fluoroquinolones against 433 strains of gram-negative bacteria isolated from 6 medical facilities in gifu prefecture between january and september in 2005, determined by the agar dilution methods in according with the japan society of chemotherapy. we also investigated the correlation between the degree of resistance to fluoroquinolones and the amino acid substitutions in the quinolone resistance-determining region (qrdr). the tested clinical isolates were ...200717867488
frequency of urinary tract infection in catheterized dogs and comparison of bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with urinary tract infections.to determine frequency of urinary tract infections (utis) in catheterized dogs that had intervertebral disk disease (ivdd) or disease other than ivdd and compare bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with utis.200717867973
synthesis and antimicrobial activity of some novel nucleoside analogues of adenosine and 1,3-dideazaadenosine.a number of nucleoside analogues have been synthesized and evaluated for their antibacterial and antifungal activities against staphylococcus aureus, group d streptococcus, pseudomonas aeruginosa, proteus spp., salmonella spp., aspergillus fumigatus, penicillium marneffei, candida albicans, cryptococcus neoformans, and mucor spp. the compounds 1, 4, and 6 emerged as potent antibacterial agents with mic values of 0.75, 0.38, and 0.19 microm, respectively, against group d streptococcus. further, t ...200717890082
breast abscess bacteriologic features in the era of community-acquired methicillin-resistant staphylococcus aureus epidemics.increasing rates of community-acquired methicillin-resistant staphylococcus aureus (mrsa) infections have also affected the microbial profile of breast abscesses.200717891866
the type iii secretion system of proteus mirabilis hi4320 does not contribute to virulence in the mouse model of ascending urinary tract infection.the gram-negative enteric bacterium proteus mirabilis is a frequent cause of urinary tract infections (utis) in individuals with long-term indwelling catheters or with complicated urinary tracts. the recent release of the p. mirabilis strain hi4320 genome sequence has facilitated identification of potential virulence factors in this organism. genes appearing to encode a type iii secretion system (ttss) were found in a low gc-content pathogenicity island in the p. mirabilis chromosome. this islan ...200717893161
endoscopic treatment of pituitary abscess: two case reports and literature review.pituitary abscess is a rare entity and the correct diagnosis is still difficult before surgery. more than 210 cases have been reported in the medical literature, mostly in the form of isolated case reports. we report two cases of pituitary abscess treated endoscopically and we review the literature. a 30-year-old woman and a 35-year-old man were admitted with a history of pituitary dysfunction. patient 1 presented with polyuria, polydipsia, amenorrhea, headache, and visual impairment. magnetic r ...200817899232
bacterial defluorination of 4-fluoroglutamic acid.fluorinated amino acids are used as enzyme inhibitors, mechanistic probes and in the production of pharmacologically active peptides. because enantiomerically pure 4-fluoroglutamate is difficult to prepare, the selective degradation of the l-isomer is a potentially convenient method of obtaining d-4-fluoroglutamate from the racemate. in this paper, we describe our investigations on the degradation of 4-fluoroglutamate by bacteria. fluoride ion was detected in resting-cell cultures of a number of ...200717901951
pattern of pathogens in ear discharge of hiv-infected children in nnewi, southeast nigeria.this is a descriptive, prospective, hospital-based study designed to determine the prevalent organisms in ear infections and their antimicrobial susceptibility patterns in hiv-infected children compared to age-and sex-matched hiv sero-negative patients.200717902505
purification, crystallization, x-ray diffraction analysis and phasing of an engineered single-chain pvuii restriction endonuclease.the restriction endonuclease pvuii from proteus vulgaris has been converted from its wild-type homodimeric form into the enzymatically active single-chain variant scpvuii by tandemly joining the two subunits through the peptide linker gly-ser-gly-gly. scpvuii, which is suitable for the development of programmed restriction endonucleases for highly specific dna cleavage, was purified and crystallized. the crystals diffract to a resolution of 2.35 a and belong to space group p4(2), with unit-cell ...200717909283
septic postpartum uterine inversion.puerperal uterine inversion is an uncommon but life threatening obstetrical emergency. a 26-year-old woman, para six, was referred from a peripheral hospital seven days after delivery, with a mass protruding per vaginum. complete uterine inversion had occurred after delivery of baby and placenta. she was resuscitated and her genital infection was treated. she had a vaginal hysterectomy upon request. her postoperative recovery was uneventful. poor management of the third stage of labour is a comm ...200717909681
production of l-carnitine by secondary metabolism of bacteria.abstract: the increasing commercial demand for l-carnitine has led to a multiplication of efforts to improve its production with bacteria. the use of different cell environments, such as growing, resting, permeabilized, dried, osmotically stressed, freely suspended and immobilized cells, to maintain enzymes sufficiently active for l-carnitine production is discussed in the text. the different cell states of enterobacteria, such as escherichia coli and proteus sp., which can be used to produce l- ...200717910757
antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent.the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ...200717913083
prevalence of extended-spectrum beta-lactamases in proteus mirabilis in a taiwanese university hospital, 1999 to 2005: identification of a novel ctx-m enzyme (ctx-m-66).a total of 1574 nonduplicate proteus mirabilis isolates collected at a taiwanese hospital during 1999 to 2005 were analyzed for production of extended-spectrum beta-lactamases (esbls). forty-four esbl-producing isolates including 22 ctx-m-14, 18 ctx-m-3, 2 ctx-m-24, and 2 ctx-m-66 producers were detected, and the proportion of esbl producers increased from 0.7% in 1999 to approximately 6% after 2002. ctx-m-66 is a novel variant of ctx-m esbls that differs from ctx-m-3 by a ser to asn change at a ...200817913434
effect of natural preservatives on the growth of histamine producing bacteria.present study deals with the hampering of the growth of histamine producing bacteria (hpb), by using nacl and spices which are easily available and cheaper cost wise. for this experiment, four strains of hpb viz. vibrio parahaemolyticus, bacillus cereus, pseudomonas aeruginosa and proteus mirabilis were tested against 1 to 10% concentrations of nacl and 1 to 5% concentrations of natural preservatives (turmeric, ginger and garlic) in a basal medium. hpb showed different growth rates at different ...200717915763
cowden syndrome.cowden syndrome (cs), due to germline mutations of the pten tumor-suppressor gene, is an often overlooked cancer predisposition syndrome associated with an increased risk of breast, thyroid, and endometrial cancers, as well as benign manifestations. germline pten mutations also are associated with syndromes that have not been historically connected to an increase in risk for malignancy. these disorders include bannayan-riley-ruvalcaba syndrome (brrs), proteus syndrome (ps), and proteus-like synd ...200717920899
bacterial contaminations of informally marketed raw milk in ghana.summary200717925843
brain abscess in infants and children: a retrospective study of 107 patients in northeast thailand.to review the clinical manifestation, predisposing factor location of abscess, causative organism, treatment, and outcome of brain abscess.200717926990
[phosphatase activity in amoeba proteus at ph 9.0].in the free-living amoeba amoeba proteus (strain b), after paag disk-electrophoresis of the homogenate supernatant, at using 1-naphthyl phosphate as a substrate and ph 9.0, three forms of phosphatase activity were revealed; they were arbitrarily called "fast", "intermediate", and "slow" phosphatases. the fast phosphatase has been established to be a fraction of lysosomal acid phosphatase that preserves some low activity at alkaline ph. the question as to which particular class the intermediate p ...200717933343
regulatory circuit based on autogenous activation-repression: roles of c-boxes and spacer sequences in control of the pvuii restriction-modification system.type ii restriction-modification (r-m) systems comprise a restriction endonuclease (rease) and a protective methyltransferase (mtase). after r-m genes enter a new cell, mtase must appear before rease or the chromosome will be cleaved. pvuii and some other r-m systems achieve this delay by cotranscribing the rease gene with the gene for an autogenous transcription activator (the controlling or 'c' protein c.pvuii). this study reveals, through in vivo titration, that c.pvuii is not only an activat ...200717933763
structure and mode of action of the antimicrobial peptide arenicin.the solution structure and the mode of action of arenicin isoform 1, an antimicrobial peptide with a unique 18-residue loop structure, from the lugworm arenicola marina were elucidated here. arenicin folds into a two-stranded antiparallel beta-sheet. it exhibits high antibacterial activity at 37 and 4 degrees c against gram-negative bacteria, including polymyxin b-resistant proteus mirabilis. bacterial killing occurs within minutes and is accompanied by membrane permeabilization, membrane detach ...200817935487
structure of a glucosyl phosphate-containing o-polysaccharide of proteus vulgaris o42.an o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of proteus vulgaris o42 and studied by sugar and methylation analyses along with 1h, 13c and 31p nmr spectroscopy. the following structure of the polysaccharide having a linear pentasaccharide phosphate repeating unit was established: -->3)-alpha-l-fucpnac4ac-(1-->4)-alpha-d-glcp-1-p-(o-->4)-alpha-d-glcpnac-(1-->3)-alpha-l-fucpnac4ac-(1-->3))-alpha-d-glcpnac6ac-(1--> where the degree of o-acetylation is approxima ...200717936254
antimicrobial susceptibility of klebsiella spp. and proteus spp. from various organ systems of horses, dogs and cats as determined in the bft-germvet monitoring program 2004-2006.a total of 120 isolates of klebsiella spp. and proteus spp. collected from horses and small animals (dogs and cats) were screened for their susceptibility to 24 different antimicrobial agents. klebsiella spp. were included from infections of the genital tract (gt) of horses (36 isolates) and the urinary/genital tract (ugt) from dogs and cats (17 isolates), while proteus spp. were from small animal (dogs and cats) infections of the ugt (37 strains) and the skin (incl. ear/mouth) (30 isolates). in ...200717939455
proteus syndrome in the aetiology of carpal tunnel syndrome. 200717939492
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