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extended-spectrum beta-lactamase-producing enterobacteriaceae in an italian intensive care unit: clinical and therapeutical remarks.in this study we evaluated the prevalence of enterobacteriaceae and the epidemiology of esbl+ microorganisms in an icu of our institution over a 5-year period and analyzed the clinical features and outcomes of the infections caused by these microorganisms. the most frequent esbl+ isolate was proteus mirabilis (69 isolates, 58%); a high rate of positive results in the double-disk synergy test (dds) was also recognized for klebsiella pneumoniae (52 isolates, 51%), whereas this phenomenon was obser ...200415216948
resistance rates to commonly used antimicrobials among pathogens of both bacteremic and non-bacteremic community-acquired urinary tract infection.this study examined the distribution of organisms and their antimicrobial resistance in patients admitted due to acute bacteremic and non-bacteremic community-acquired urinary tract infection (uti). during a period of 1 year and 1 month, a total of 201 patients and 253 bacterial isolates were studied. fever higher than 38.5 degrees c was significantly more common in the bacteremic group than the non-bacteremic group (68% vs 48%; p<0.05). escherichia coli, klebsiella pneumoniae, pseudomonas aerug ...200415221039
antibiotic resistance of urinary tract pathogens and rationale for empirical intravenous therapy.empirical antibiotic treatment in urinary tract infection (uti) in children must rely on surveillance data on the epidemiology and resistance patterns of common uropathogens. a retrospective analysis of bacteria isolated from children with uti irrespective of underlying disease or pre-treatment was performed at the university hospital of freiburg, germany, in 1997, and from 1999 to 2001. in the first study period, 261 positive urine samples and in the second period 684 positive samples were anal ...200415221429
sensitivity and spectrum of bacterial isolates in infectious otitis externa.to determine the spectrum and antibiotic sensitivity pattern of organisms from aural swabs taken from cases of infectious otitis externa.200415228846
central venous catheter-related blood stream infections: incidence, risk factors, outcome, and associated pathogens.to determine the incidence, risk factors, outcome, and pathogens of central venous catheter-related bloodstream infections (cvc-bsis).200415232809
changing epidemiology of neonatal septic arthritis.to study the changing epidemiological pattern of micro-organisms as an aetiology of septic arthritis, and to correlate the pattern with the outcome of neonatal septic arthritis, in terms of joint function and morphology.200415237115
the antimicrobial activity of extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents.the antimicrobial activity of the chloroform, diethyl ether, acetone, petroleum ether, and ethanol extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents against 9 bacteria and fungi has been investigated. the extracts and pure compounds alone were found active against the same bacteria and the same yeasts. bacillus cereus, bacillus subtilis, staphylococcus aureus, streptococcus faecalis, proteus vulgaris, listeria monocytogenes, aero ...200415241936
isolation of bacteriophages from the oral cavity.to isolate bacteriophages lytic for oral pathogens from human saliva, dental plaque and mature biofilms constituted from saliva-derived bacteria.200415242464
structure of the o-polysaccharide of proteus mirabilis o19 and reclassification of certain proteus strains that were formerly classified in serogroup o19.bacteria of the genus proteus are a common cause of urinary tract infections. the o-polysaccharide chain of their lps (o-antigen) defines the serological specificity of these bacteria. based on the immunospecificity of the o-antigens, two species, p. mirabilis and p. vulgaris, were classified into 49 o-serogroups, and more o-serogroups for strains of these species and p. penneri have been subsequently proposed.200415247885
bacterial dynamics associated with algal antibacterial substances during post harvest desiccation process of sargassum stolonifolium phang et yoshida.brown algae of genus sargassum are known to produce relatively higher amount of alginic acid. optimal extraction of this algalcolloid for local consumption requires in-depth studies on post-harvest treatment of the algal fronds. present investigation endeavors to establish the dynamics and inter-relationship of moisture content and bacteria found on the surface of the alga and alginic acid content during post-harvest desiccation of sargassum stolonifolium phang et yoshida. harvested fronds were ...200315248481
[urolithiasis in hemodialysis patients]. 200415250327
gene cloning, expression and characterization of novel phytase from obesumbacterium proteus.the gene phya encoding phytase was isolated from obesumbacterium proteus genomic library and sequenced. the cleavage site of the phya signal peptide was predicted and experimentally proved. the phya protein shows maximum identity of 53% and 47% to phosphoanhydride phosphorylase from yersinia pestis and phytase appa from escherichia coli, respectively. based on protein sequence similarity of phya and its homologs, the phytases form a novel subclass of the histidine acid phosphatase family. to cha ...200415251209
lemierre's syndrome associated with septic pulmonary embolism: a case report.an 18-year-old female patient suffering from a painful right-sided neck mass was admitted to our emergency service. computed tomography of the neck revealed thrombosis of the right internal jugular vein. computed tomography of the chest indicated septic pulmonary emboli in both lungs. blood and oropharyngeal cultures showed proteus that was sensitive to cefepime and amicasin. chemotherapy was administered for 12 days, however, her complaints continued with fulminant progression. an urgent decomp ...200415253264
in vitro activity of ave1330a, an innovative broad-spectrum non-beta-lactam beta-lactamase inhibitor.production of beta-lactamases is the main mechanism of beta-lactam resistance in gram-negative bacteria. despite the current use of clavulanic acid, sulbactam and tazobactam, the prevalence of class a and class c enzymes is increasing worldwide, demanding new beta-lactamase inhibitors. here we report the antimicrobial properties of ave1330a, a representative of a novel class of bridged bicyclico[3.2.1]diazabicyclo-octanones in combination with ceftazidime. materials and methods: ic(50) and kinet ...200415254025
kirby-bauer disc approximation to detect inducible third-generation cephalosporin resistance in enterobacteriaceae.resistance to beta-lactam antibiotics in enteric gram-negative bacilli may be difficult to detect using standard methods of either kirby-bauer disc diffusion (kbdd) or broth dilution for minimal inhibitory concentration (mic). this difficulty is due to genetic differences in resistance determinants, differences in levels of gene expression, and variation in spectra of enzymatic activity against the substrate beta-lactams used for susceptibility testing. we have examined 95 clinical isolates repo ...200415256000
radiologic manifestations of proteus syndrome.proteus syndrome is a sporadic disorder named for its highly variable manifestations. the disease causes tissue overgrowth in a mosaic pattern and may affect tissues derived from any germinal layer. the disease process is not usually apparent at birth but develops rapidly in childhood. common manifestations include macrodactyly, vertebral abnormalities, asymmetric limb overgrowth and length discrepancy, hyperostosis, abnormal and asymmetric fat distribution, asymmetric muscle development, connec ...200415256628
diffuse xanthogranulomatous pyelonephritis and staghorn calculus: report of one case.xanthogranulomatous pyelonephritis and staghorn calculus are rare in children. in this report, we describe a chinese boy without history of urinary tract infection who developed insidious onset of left flank pain. urine culture showed proteus mirabilis infection. sonography and computed tomography of the abdomen showed typical picture of xanthogranulomatous pyelonephritis and staghorn calculus. photomicrography showed characteristic lipid-laden macrophage aggregates. after nephrectomy, he was sy ...200415264707
lesson of the week: perils of pessaries.vaginal pessaries are widely considered to be a safe alternative to surgery in older women. we report a case of near fatal septicaemia in a 75-year-old woman associated with a shelf pessary, the presence of which was identified during an exploratory laparotomy. this case highlights the importance of the gynaecological history and examination when assessing older women with septicaemia of unknown source.200415271640
the three-dimensional structure of catalase from enterococcus faecalis.enterococcus faecalis haem catalase was crystallized using lithium sulfate at neutral ph. the crystals belong to space group r3, with unit-cell parameters a = b = 236.9, c = 198.1 a. the three-dimensional structure was determined by molecular replacement using a subunit of the proteus mirabilis catalase structure. it was refined against 2.3 a synchrotron data to a free r factor of 21.8%. like other catalases, the e. faecalis catalase is a homotetramer with a fold and structure similar to those o ...200415272159
incidence of enteric bacteria and staphylococcus aureus in day care centers in akwa ibom state, nigeria.the incidence of enteric bacteria and staphylococcus aureus in four day care centers in akwa ibom state was studied using culture techniques. the percentage frequencies of the isolates from 124 samples were staphylococcus aureus (33.9), escherichia coli (19.0), klebsiella sp (14.4), citrobacter sp (12.5) and proteus mirabilis (7.4). the sources of contamination were floors, chairs, skin, bed linen, door handles, fans, children's tables, walls, windows, ceiling, headmistress's table and chairs, d ...200415272770
application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections.a rapid and accurate method for detection for common pathogenic bacteria in foodborne infections was established by using oligonucleotide array technology. nylon membrane was used as the array support. a mutation region of the 23s rrna gene was selected as the discrimination target from 14 species (genera) of bacteria causing foodborne infections and two unrelated bacterial species. a pair of universal primers was designed for pcr amplification of the 23s rrna gene. twenty-one species (genera)-s ...200415279944
structure of the o-polysaccharide of proteus serogroup o34 containing 2-acetamido-2-deoxy-alpha-d-galactosyl phosphate.on mild acid degradation of the lipopolysaccharide of proteus vulgaris o34, strain ccug 4669, the o-polysaccharide was cleaved at a glycosyl-phosphate linkage that is present in the main chain. the resultant phosphorylated oligosaccharides and an alkali-treated lipopolysaccharide were studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, and the following structure of the branched tetrasaccharide phosphate repeating unit of the o-polysaccharide was established: [carbo ...200415280059
frequency and diversity of class a extended-spectrum beta-lactamases in hospitals of the auvergne, france: a 2 year prospective study.to evaluate the frequency and diversity of extended-spectrum beta-lactamases (esbls) produced by enterobacteriaceae and pseudomonas aeruginosa in one french region.200415282240
epidermal nevus syndromes: clinical findings in 35 patients.of the patients with epidermal nevi, 10-18% may have disorders of the eye, nervous, and musculoskeletal systems. a predisposition to malignant neoplasms in ectodermal and mesodermal structures may also be found. there are six different epidermal nevus syndromes described so far: proteus, congenital hemidysplasia with ichthyosiform nevus and limb defect syndrome, phakomatosis pigmentokeratotica, sebaceous nevus, becker nevus, and nevus comedonicus. thirty-five patients with epidermal nevus syndro ...200415283784
anesthesia for proteus syndrome.proteus syndrome is a complex disorder comprising malformations and overgrowth of multiple tissues. the disorder is highly variable affecting tissues in a mosaic pattern. a 2-year-old boy with proteus syndrome, with epidermal verrucal naevus, hyperplastic lesions of connective tissue, hyperostosis, overgrowth of tubular bones, bilateral inguinal hernia, and juvenile intestinal polyposis was scheduled for vertebral magnetic resonance imaging (mri) for further evaluation of malignancies. in additi ...200415283831
emerging resistance among bacterial pathogens in the intensive care unit--a european and north american surveillance study (2000-2002).globally icus are encountering emergence and spread of antibiotic-resistant pathogens and for some pathogens there are few therapeutic options available.200415283864
epidermal nevus syndromes.the term "epidermal nevus syndrome" (ens) has been used to describe the association of epidermal hamartomas and extra-cutaneous abnormalities. epidermal nevi follow the lines of blaschko. the majority of the extra-cutaneous manifestations involve the brain, eye, and skeletal systems. several subsets with characteristic features have been delineated including the nevus sebaceous syndrome, proteus syndrome, child syndrome, becker nevus syndrome, nevus comedonicus syndrome, and phakomatosis pigment ...200415295924
investigating the antimicrobial activity of natural honey and its effects on the pathogenic bacterial infections of surgical wounds and conjunctiva.antimicrobial activities of 10-100% (wt/vol) concentrations of new honey, stored honey, heated honey, ultraviolet-exposed honey, and heated stored honey were tested against common human pathogens, including escherichia coli, entrobacter cloacae, pseudomonas aeruginosa, shigella dysenteriae, klebsiella sp., haemophilus influenzae, proteus sp., staphylococcus aureus, streptococcus hemolyticus group b, and candida albicans. antimicrobial activity of honey was tested in acidic, neutral, or alkaline ...200415298770
crystallization and preliminary x-ray analysis of a bacterial glutathione transferase.crystals of a bacterial glutathione s-transferase from proteus mirabilis have been grown from polyethylene glycol by the hanging-drop vapour-diffusion method. successful crystallization required the presence of the substrate glutathione. the crystals belong to the tetragonal space group p4 with cell dimensions a = b = 90.9 and c = 117.3 a. they contain between three and six monomers in the asymmetric unit and diffract to beyond 2.3 a resolution.199615299741
[synthesis and antibacterial activity of 2-(3-pyridyl)-5-([(5-aryl-1,3,4-oxadiazol-2-yl) methylene]thio)-1,3,4-oxadiazoles].studies on synthesis and antibacterial activity of new heterocycles.200415303654
treatment of dairy wastewater using a selected bacterial isolate, alcaligenes sp. mmrr7.physicochemical and biologic analysis of dairy wastewater showed that the effluent had a high organic load (chemical oxygen demand [cod]: 5095 mg/l), an acidic ph (6.4), and a high probability of coliforms (most probable number [mpn] >1100). the various bacterial strains isolated and purified were identified as sporolactobacillus sp., citrobacter sp., pseudomonas sp., alcaligenes sp., bacillus sp., staphylococcus sp., and proteus sp., as per the bergey's manual of systematic bacteriology. among ...200415304739
[bacterial infections in chronic otitis media--usefulness of molecular diagnostics based on pcr method].infections in chronic otitis media (com) are common, and identification of their pathogen is difficult, what is proven by high rate of negative results of bacteriological examinations. this fact may be explained by two factors: it is difficult to obtain a reliable material for culture and classic methods of bacteriological cultures may be of low sensitivity. amplification of bacterial dna in pcr is an examination, that leads to a higher effectiveness and sensitivity of bacteriological tests. the ...200415311593
cutaneous manifestations of proteus syndrome: correlations with general clinical severity.proteus syndrome is a rare congenital disorder with progressive asymetric overgrowth of multiple tissues.200415313810
the manifold faces of proteus syndrome. 200415313819
contribution of the two conserved tryptophan residues to the catalytic and structural properties of proteus mirabilis glutathione s-transferase b1-1.pmgstb1-1 (proteus mirabilis glutathione s-transferase b1-1) has two tryptophan residues at positions 97 and 164 in each monomer. structural data for this bacterial enzyme indicated that trp97 is positioned in the helix a4, whereas trp164 is located at the bottom of the helix a6 in the xenobiotic-binding site. to elucidate the role of the two tryptophan residues they were replaced by site-directed mutagenesis. trp97 and trp164 were mutated to either phenylalanine or alanine. a double mutant was ...200515320869
s-adenosylmethionine and pneumocystis.pneumocystis is a parasitic fungus causing pneumonia in immunosuppressed mammals and s-adenosylmethionine a key intermediary metabolite for all cells. other than a species of rickettsia bacteria and an aberrant strain of the protozoan amoeba proteus, pneumocystis is the only cell known unable to synthesize adomet; it must extract this key compound from its host. this was discovered using a culture system and confirmed by observing depletion of adomet in the plasma of infected animals. depletion ...200415321660
a novel glutamate-dependent acid resistance among strains belonging to the proteeae tribe of enterobacteriaceae.morganella, providencia and proteus strains were capable of surviving ph 2.0 for 1 h if glutamate was present. these strains did not have glutamic acid decarboxylase activity and the gadab genes were not detected in any of these bacteria. when exposed to ph 2.0 acid shocks, the survival rate of these bacteria was significantly increased with glutamate concentrations as low as 0.3 mm in the acid media. escherichia coli cells incubated at ph 3.4 consumed four times more glutamate and produced at l ...200415321677
proteus mirabilis zapa metalloprotease degrades a broad spectrum of substrates, including antimicrobial peptides.the 54-kda extracellular metalloprotease zapa is an important virulence factor of uropathogenic proteus mirabilis. while zapa has the ability to degrade host immunoglobulins (igs), the dramatic attenuation of virulence in zapa mutants suggests that this enzyme may have a broader spectrum of activity. this hypothesis was tested by in vitro assays with purified zapa and an array of purified protein or peptide substrates. the data reveal that many proteins found in the urinary tract are substrates ...200415322010
incidence of extended-spectrum beta-lactamases in clinical isolates of the family enterobacteriaceae in a pediatric hospital.the incidence of extended-spectrum beta-lactamases (esbls) was analyzed in enterobacteriaceae population circulating in the upper silesian child and mother health center in katowice (usc&mhc). altogether 1164 clinical specimens, collected from children hospitalized in 8 different hospital units of usc&mhc were investigated. five hundred and eighty-five clinical isolates of the family enterobacteriaceae were identified in specimens collected from 403 patients. two hundred and twenty-nine enteroba ...200415330264
per-1 production in a urinary isolate of providencia rettgeri.a providencia rettgeri strain resistant to extended-spectrum cephalosporins and intermediate to aztreonam was isolated from the urine of a patient hospitalized in the urology clinic of ssk educational hospital in ankara. clavulanic acid restored the activity of extended-spectrum cephalosporins, suggesting that the strain was harboring an extended-spectrum beta-lactamase. since the per-1 enzyme is widespread in turkey, and had been already detected in a related species such as proteus mirabilis, ...200415332708
urinary calculi: bacteriological and chemical association.we investigated the bacteriology of urinary calculi in relation to urinary tract infection, stone formation, chemical composition and antibiotic sensitivity. fifty-two patients (37 males, 15 females) with urolithiasis were studied. urine, serum and urinary calculi specimens were taken and serum biochemical tests to detect uric acid, calcium and phosphorus were performed. urine analysis and culture were also performed. of the 52 patients, 19 (37%) had associated urinary tract infection, with esch ...200115332777
defense function of pigment granules in the ciliate blepharisma japonicum against two predatory protists, amoeba proteus (rhizopodea) and climacostomum virens (ciliata).the defense function of pigment granules in the red ciliate blepharisma japonicum against two predatory protists, amoeba proteus and climacostomum virens, was investigated by (1) comparing normally-pigmented and albino mutant cells of b. japonicum as the prey of these predators and (2) comparing resistance of the predators to blepharismin, the toxic pigment contained in the pigment granules of b. japonicum. normally pigmented cells which contained more blepharismin than albino cells were less vu ...200415333994
klippel-trénaunay syndrome: the importance of "geographic stains" in identifying lymphatic disease and risk of complications.klippel-trénaunay syndrome (kts) is a rare congenital anomaly classically defined as the triad of vascular stain, soft tissue and/or bony hypertrophy, and venous varicosities.200415337982
effects of trifluoromethyl ketones on the motility of proteus vulgaris.in the present study, we showed the inhibition of motility by trifluoromethyl ketone (tf) derivatives (1-8) in proteus vulgaris (p. vulgaris) cultures. among them, 1-(2-benzoxazoyl)-3,3,3-trifluoro-2-propanone (1) showed a much stronger inhibitory effect on the motility of p. vulgaris than other tf compounds at 10% mic. our results suggest the possibility of an inhibitory action of tf compounds on the proton motive forces by affecting the action of biological motor and proton efflux in the membr ...200415340240
[causes of urinary tract infection in pregnant women].data of etiology characteristics of uroinfections with pregnant women are presented treated at university hospital of obstetrics and gynecology "maichin dom" in three periods. gram negative species of microorganisms (e. coli, proteus, klebsiella) are the agens of uroinfections in 95.4% of cases in the period 1988/89. after 10-year period gram (-) are the uroinfection in 66.1% of the cases. the growing etiologycal importance of gram positive species of microorganisms (s. aureus, enterococcus, gbs ...200415341251
[incidence and sensitivity to antibiotics of germs isolated from surgical wound infections].we studied 119 bacterial strains isolated from postoperative infected wounds. all these strains were considered to be strains that derived from the same number of nosocomial infections, all the postoperative infections of the wounds being nosocomial infections. regarding their frequency, we isolated the following strains: e. coli--68 strains (57%), s. aureus--37 strains (31%), pseudomonas spp.--9 strains (8%) and proteus spp.--5 strains (4%). we performed the bacteriological study of these strai ...200315341326
[molecular mechanisms of cephalosporin resistance in gram-negative bacteria of the enterobacteriaceae family].extended spectrum beta lactamase genes were detected by the pcr in 87.6% of 231 enterobacteriaceae strains isolated in medical institutions of moscow, st. petersburg, tomsk and nazran that showed a decrease in their susceptibility to 3rd generation cephalosporins. alone or in various combinations tem type beta lactamases were detected in 43.3% of the isolates, 46.8 and 51.2% of the isolates produced shv type and ctx type beta lactamases respectively. combinations of 2 and 3 different determinant ...200415344391
[use of therapeutic polycomponent vaccine "immunovac vp-4" for the treatment of chronic bacterial prostatitis].a complex of clinical and laboratory examinations of patients with bacterial chronic prostatitis was carried out. the patients were divided into two groups: 55 patients treated by the method of immunotherapy and 20 patients (controls) undergoing basic therapy. immunotherapy was carried out with the use of polycomponent vaccine vp-4 containing staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris and escherichia coli antigens. vaccinal therapy was indicated in cases with infection focus ...200415346948
transcription of proteus mirabilis flaab.proteus mirabilis, a gram-negative urinary tract pathogen, has two highly homologous, tandemly arranged flagellin-encoding genes, flaa and flab. flaa is transcribed from a sigma(28) promoter, while flab is a silent allele. previous studies have demonstrated the presence of a family of hybrid flagellin genes, referred to as flaab. these genes are composed of the 5' end of flaa and the 3' end of flab, and are produced through excision of the intervening dna between the two genes. although the exis ...200415347745
digestive pathology of sea turtles stranded in the canary islands between 1993 and 2001.digestive lesions were observed in 84 of 136 sea turtles (128 caretta caretta, four chelonia mydas and four dermochelys coriacea) stranded in the canary islands between january 1993 and december 2001. in the oral cavity ulcerative and necropurulent stomatitis were the most frequently observed lesions, and in the oesophagus ulcerative and fibrinous oesophagitis, and traumatic oesophageal perforation were most frequently observed; all these lesions were mainly associated with the ingestion of fish ...200415357377
poly(vinyl chloride)-coated sol-gels for studying the effects of nitric oxide release on bacterial adhesion.nitric oxide (no) releasing sol-gel materials coated with poly(vinyl chloride) (pvc) films exhibit increased stability at ambient and physiological temperatures. the polymer overcoat, however, reduces the no fluxes by 5-35% over the initial week of release. the variation in no fluxes between unmodified and pvc-coated sol-gels is negligible after 7 days. the pvc polymeric layer provides controlled surface chemistry for systematic studies of the effects of no release on bacterial adhesion. as an e ...200415360321
surgical infections: a microbiological study.surgical infections are mostly polymicrobial, involving both aerobes and anaerobes. one hundred seventeen cases comprised of abscesses (n=51), secondary peritonitis (n=25), necrotizing fascitis (n=22) and wounds with devitalized tissues (n=19) were studied. the number of microorganisms isolated per lesion was highest in secondary peritonitis (2.32). the aerobe/ anaerobe ratio was 0.81 in secondary peritonitis and 1.8 in necrotizing fascitis. most secondary peritonitis (80%), necrotizing fascitis ...200415361989
bacterial resistance to antimicrobials in urinary isolates.escherichia coli accounted for about 80% of organisms in uncomplicated urinary tract infections (utis), followed by staphylococcus spp. especially staphylococcus saprophyticus, and proteus mirabilis. against e. coli isolates from patients with uncomplicated uti, faropenem was the most effective. up to 1999, fluoroquinolone-resistant isolates were not observed in patients with uncomplicated uti, but in 2001 fluoroquinolone-resistant e. coli isolates emerged and accounted for about 8%. various typ ...200415364302
urinary tract infections in african infants.urinary tract infection (uti) causes significant illness in the first 2 years of life. the diagnosis in most developing countries is often overlooked due to the tedious nature of obtaining urine from these young ones who would not void voluntarily. misdiagnosis very often lead to avoidable ill health and long-term renal damage. there is a need to diagnose uti in febrile infants to alert clinicians. a prospective study of febrile infants aged up to 12 months on admission was undertaken in a 6-mon ...200415364303
the role of unusual pathogens in prostatitis syndrome.a total of 1442 patients with symptoms of chronic prostatitis were examined over a 4-year period at the outpatient department for urogenital infections, university hospital for infectious diseases "dr. fran mihaljevic", zagreb, croatia. an infectious aetiology was determined in 1070 (74.2%) patients. in 561 of 1070 (52.4%) patients the inflammatory finding (>10 wbc/hpf) was found in expressed prostatic secretions (eps) or voided bladder urine (vb(3)). normal, <10 wbcs/hpf was found in 362 of 536 ...200415364308
nosocomial transmission of ctx-m-2 beta-lactamase-producing acinetobacter baumannii in a neurosurgery ward.three strains of cefotaxime (ctx)-resistant acinetobacter baumannii, fm0209680, fm0300106, and fm0301433, were isolated from transtracheal aspirate cultures of three patients with probable nosocomial infections in a neurosurgery ward in japan. the ctx mics for these isolates were greater than 128 microg/ml but were drastically reduced in the presence of 4 microg of clavulanic acid per ml. these strains were also resistant to ceftriaxone, cefpodoxime, and aztreonam but were susceptible to ceftazi ...200415364979
microbiological quality of fermented cassava flour 'kpor umilin'.a study was conducted on the microbiological quality of traditionally fermented cassava flour 'kpor umilin' and a laboratory modification of the method developed with a view to upgrading the traditional process. microbial analysis of samples from both processing methods was evaluated using standard microbiological methods. the bacterial count in the traditionally processed flours ranged from 2.7 x 10(3) to 1.2 x 10(7) cfu/g, while the modified sample had a count of 3.5 x 10(2) cfu/g. the predomi ...200415369985
diagnosis of proteus syndrome was correct. 200415372511
mutation analysis of the tumor suppressor pten and the glypican 3 (gpc3) gene in patients diagnosed with proteus syndrome.proteus syndrome is a complex hamartomatous disorder characterized by asymmetrical gigantism, epidermal nevi, vascular malformations, hamartomas, lipomas, and hyperostosis. since the syndrome was first described, many hypotheses have been proposed to explain its occurrence. the most plausible is happle's somatic mosaic hypothesis, but no somatic mutations in candidate genes have been reported to be clearly involved in proteus syndrome. however, germ-line pten mutations have been reported in pati ...200415372512
reassessment of the proteus syndrome literature: application of diagnostic criteria to published cases.the medical care of patients affected by rare disorders depends heavily on experiences garnered from prior cases, including those patients evaluated by the treating physician and those published in the medical literature. the utility of published cases is wholly dependent upon accurate diagnosis of those patients. in our experience, the rate of misdiagnosis in proteus syndrome (ps) is high. diagnostic criteria have been published, but these criteria have not been applied consistently and were pu ...200415372514
[urinary stones in tunisian infants, based on a series of 64 cases].to define the epidemiological and clinical characteristics of urinary stones in infants, to study the role of stone chemical analysis in the aetiological assessment of urinary stones and to define the various treatment modalities adapted to this age-group.200415373180
mucosal vaccination of mice with recombinant proteus mirabilis structural fimbrial proteins.proteus mirabilis, a common cause of urinary tract infection (uti), expresses several types of fimbria including mannose-resistant/proteus-like fimbriae (mrp), uroepithelial cell adhesin (uca), renamed non-agglutinating fimbriae (naf) by some authors, and p. mirabilis fimbriae (pmf), which are potentially involved in adhesion to the uroepithelium. in this study, we immunised different groups of mice with recombinant structural subunits of these fimbriae (mrpa, ucaa and pmfa) using two mucosal ro ...200415374007
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2002). i. susceptibility distribution].the bacterial strains isolated from 491 patients diagnosed as having urinary tract infections (utis) in 13 institutions in japan were supplied between august 2002 and july 2003. the susceptibilities of them to many kinds of antimicrobial agents were investigated. of them, 578 strains were estimated as causative bacteria and used for the investigation. the number of them was 578 strains consisting of 177 gram-positive bacterial strains (30.6%) and 401 gram-negative bacterial strains (69.4%). agai ...200415376785
[xanthogranulomatous pyelonephritis: clinical, radiological and pathologic characteristics].xanthogranulomatous pyelonephritis is an atypical chronic infection which simulates a malignant renal neoplasia, definitive diagnosis of which is obtained by pathologic study after surgical removal. in this paper we analyze our experience and compare it to that reported on the literature.200415382434
[profile of antimicrobial resistance of agents causing urinary tract infections in children].the management of urinary tract infection in children faces the problem of the emergence of resistant strains to antibiotics. the aim of this study is to precise the frequency of the different germs and their susceptibility to antibiotics.200415382465
in vitro antimicrobial properties of aqueous garlic extract against multidrug-resistant bacteria and candida species from nigeria.the antimicrobial effects of aqueous garlic extract (age) against 133 multidrug-resistant gram-positive and gram-negative bacterial isolates, including staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, haemophilus influenzae, salmonella typhi, pseudomonas aeruginosa, escherichia coli, shigella spp., and proteus spp., and against 10 candida spp. were studied. antibacterial activity of age by well-diffusion and macrobroth dilution method was chara ...200415383227
the effect of podophyllotoxin on tissue metabolism and enzyme systems.podophyllotoxin, 10(-3) (m), inhibits the respiration in vitro of rat lymph nodes, thymus, kidney, tumor, spleen, liver, brain, testis, and chicken embryo. lymph node and spleen respiration are most sensitive, and the degree of inhibition increases with time. the injection of podophyllotoxin into tumor-bearing mice (20 mg. per kg.) causes a dramatic reduction in the respiration of tumor slices. within 6 hours, the respiration approaches zero. inhibition is evident 2 hours after injection of the ...194915394071
cobalt and bacterial growth, with special reference to proteus vulgaris. 194915395181
studies on the antigenic substance common to proteus ox10, rickettsia prowazeki and rickettsia mooseri. 194915396952
the effect of cobalt on the relationship between nucleic acid concentration and growth rate in proteus vulgaris. 194915400375
effect of cobalt on the phosphorus turnover rate in the nucleic acids of proteus vulgaris. 194915400376
in vitro sensitivity of bacillus proteus and pseudomonas aeruginosa to seven antibiotics; penicillin, streptomycin, bacitracin, polymyxin, aerosporin, aureomycin, and chloromycetin. 195015404235
streptomycin in the treatment of proteus vulgaris meningitis. 195015413915
mural bacterial endocarditis produced by proteus. 195015415278
the morphology and motility of proteus vulgaris and other organisms cultured in the presence of penicillin. 195015415581
[clinicopathological sessions; septicemia due to proteus mirabilis]. 195015420285
dispersal of amoeba proteus. 195015423557
[preparation of antigens for the diagnosis and therapy of bacterial allergy. iv. antigens of enterobacteria (escherichia, aerobacter, klebsiella, proteus, salmonella, shigella)]. 195015427975
demonstration of the chromatinic bodies of escherichia coli and proteus vulgaris with the aid of the phase contrast microscope. 195015436463
infective organisms living in the cytoplasm of amoeba proteus. 196015445238
incidence and susceptibility to antibiotics of bacteria causing common infections in a general hospital. 196315446089
thermorubin, a new antibiotic from a thermoactinomycete. 196415446134
otitis externa infection in jordan. clinical and microbiological features.aims of this study were to investigate the clinical findings and to identify causative microorganisms and their susceptibility to antibiotics in otitis externa infection.200415448765
prulifloxacin.prulifloxacin, the prodrug of ulifloxacin, is a broad-spectrum oral fluoroquinolone antibacterial agent. after absorption, prulifloxacin is metabolised by esterases to ulifloxacin. the drug has a long elimination half-life, allowing once-daily administration. ulifloxacin is generally more active in vitro than other fluoroquinolones against a variety of clinical isolates of gram-negative bacteria, including community and nosocomial isolates of escherichia coli, klebsiella spp., proteus, providenc ...200415456336
[bacterial colonization and home mechanical ventilation: prevalence and risk factors].to investigate the prevalence of bacterial contamination of ventilators and colonization of patients, the bacteria implicated, and predisposing factors in noninvasive home ventilation.200415458614
pcr method based on the ogdh gene for the detection of salmonella spp. from chicken meat samples.in a previous paper, the ogdh gene that encodes 2-oxoglutarate dehydrogenase was isolated from salmonella typhimurium. the catalytic n-terminal region in the enzyme was found to be very specific for the salmonella species. therefore, the aim of the present study was to detect s. typhimurium in food sources using primers designed for ogdh-1 and ogdh-2 which were based on the salmonella-specific region of the ogdh gene. a simple polymerase chain reaction (pcr) detection method was developed to det ...200415459651
[antimicrobial activity of new original 2-aryliden-6-furfuryliden cyclohexanones and hexahydroindazoles on their basis].antimicrobial activity of 25 new original 2-aryliden-6-furfuryliden cyclohexanones and hexahydroindazoles based on them was studied. the majority of the compounds had low or moderate activity against the test cultures of staphylococcus spp., pseudomonas aeruginosa, proteus spp. and escherichia coil. compound xix (2,3-diphenyl-7-(5-nitrofurfuryliden)-3,3a,4,5,6,7-hexahydroindazole) with high antistaphylococcal activity was selected among several 2-aryliden-6-furfuryliden cyclohexanones and hexahy ...200415460019
species-dependent premature degradation of absorbable suture materials caused by infection--impact on the choice of thread in vascular surgery.in case of infection after vascular reconstruction, preference is often given to absorbable suture material with the aim of preventing persistence of infection. we have investigated the functional deficit of absorbable sutures on incubation with various different bacteria.200415461069
[microbiocenosis of the bowels in patients with gastroduodenal ulcers].gastroduodenal ulcer is accompanied by the bowels dysbacteriosis irrespective of the helicobacter pylori infection. any variant of ulcer treatment aggravates the bowels dysbacteriosis intensity. eradication therapy destroys bifidobacteria mostly.200415462314
correlation of extended spectrum beta lactamases production with cephalosporin resistance in gram negative bacilli.beta lactamase production is an important mechanism of developing resistance to beta lactam group of antibiotics. cephlosporins with extended spectrum of activity and stability were introduced to overcome this resistance, but soon production of extended spectrum beta lactamases (esbls), which are inducible in nature was reported. in this study klebsiella aerogenes--166, escherichia coli--120, citrobacter spps--116, pseudomonas spps--50, proteus spps--32 and s. typhi--16, strains were subjected t ...200415471144
digestion of cellulose and xylan by symbiotic bacteria in the intestine of the indian flying fox (pteropus giganteus).bats (order chiroptera) are a widely distributed group of mammals. pteropus giganteus belongs to the suborder megachiroptera. this bat consumes fruits and leaves as their major food. cellulose and xylan are the major composition of leaves. as they consume leaves in their diet, their digestive tract must contain cellulolytic and xylanolytic bacteria which help in the digestion of cellulose and xylan. the cellulolytic and xylanolytic bacteria were isolated and screened on berg's agar containing ce ...200415471682
evaluation of the microscan esbl plus confirmation panel for detection of extended-spectrum beta-lactamases in clinical isolates of oxyimino-cephalosporin-resistant gram-negative bacteria.we aimed to assess the performance of the microscan esbl plus confirmation panel using a series of 87 oxyimino-cephalosporin-resistant gram-negative bacilli of various species.200415471997
[antibodies to yersinia enterocolitica and proteus mirabilis in blood sera of rheumatoid arthritis patients].data on specific antibodies to the bacterial antigens of yersinia enterocolitica (serovars 03, 09) and proteus mirabilis in rheumatoid arthritis (ra) patients are presented. according to the data of some researchers, these organisms play some role in the pathogenesis of ra. the highest level of igg antibodies to y. enterocolitica antigens of both serovars was noted. the activity of specific igg antibodies to serovar 03 was higher than that to serovar 09. in the sera of ra patients with p. mirabi ...200415481928
detection of staphylococcus aureus enterotoxin a and b genes with pcr-eia and a hand-held electrochemical sensor.two electrochemical assays for detecting staphylococcus aureus enterotoxin a and b genes were developed. the assays are based on pcr amplification with biotinylated primers, hybridization to a fluorescein-labeled probe, and detection with horseradish peroxidase-conjugated anti-fluorescein antibody using a hand-held electrochemical detector. the limit of detection (lod) for both assays was approximately 16 copies of the sea and seb genes. the assays were evaluated in blinded studies, each with 81 ...200415488376
[microbiological quality of minimally processed vegetable salads].the microbiological condition of minimally processed vegetable salads (7-days durability) purchased in the retail network of szczecin has been assessed. the study included 14 kinds of salads manufactured by two producers. the total count of mesophilic aerobic bacteria and their spores, aid-forming bacteria (lactobacilli), titre of coliforms, occurrence of pathogenic bacteria and counts of moulds and yeast were determined. no pathogenic bacteria (salmonella, staphylococcus aureus, bacillus cereus ...200415493346
micredox--development of a ferricyanide-mediated rapid biochemical oxygen demand method using an immobilised proteus vulgaris biocomponent.biochemical oxygen demand (bod) is an international regulatory environmental index for monitoring organic pollutants in wastewater and the current legislated standard test for bod monitoring requires 5 days to complete (bod5 test). we are developing a rapid microbial technique, micredox, for measuring bod by eliminating oxygen and, instead, quantifying an equivalent biochemical co-substrate demand, the co-substrate being a redox mediator. elevated concentrations of proteus vulgaris, either as fr ...200415494235
microorganisms and calcium oxalate stone disease.microorganisms may have a role in the pathogenesis and prevention of kidney stones. the subjects of this review include nanobacteria, oxalobacter formigenes, and lactic acid bacteria. not reviewed here is the well-described role of infections of the urinary tract with proteus species and other urease-producing organisms associated with struvite stone formation. nanobacteria have been proposed to be very small (0.08-0.5 nm), ubiquitous organisms that could play a role in stone formation. the theo ...200415499215
four variants of the citrobacter freundii ampc-type cephalosporinases, including novel enzymes cmy-14 and cmy-15, in a proteus mirabilis clone widespread in poland.twenty-nine proteus mirabilis isolates from 17 polish hospitals were analyzed. the isolates were resistant to a variety of antimicrobials, and their patterns of resistance to beta-lactams resembled those of the constitutive class c cephalosporinase (ampc) producers. indeed, beta-lactamases with a pi of approximately 9.0 were found in all of the isolates, and they were subsequently identified as four ampc-type cephalosporinases, cmy-4, -12, -14, and -15, of which the two last ones were novel enzy ...200415504832
synthesis and antimicrobial activity of some netropsin analogues.nine novel lexitropsins were synthesized by linking two netropsin-like moieties through three different dicarboxylic acids; 9,10-dihydro-2,7-phenanthrenedicarboxylic acid; [(3-[[(carboxymethyl)amino]carbonyl]benzoyl)amino]acetic acid and indole-2,5-dicarboxylic acid. the netropsin residues were modified by the use of n-isopentylpyrrole, 5-methylthiophene or 5-isopropylthiazole heterocyclic building blocks in place of the usual n-methylpyrrole. the compounds were tested against five gram-positive ...200415505717
traditional medicine in sakarya province (turkey) and antimicrobial activities of selected species.traditional medicine in north-west of turkey (sakarya province) were studied during a 2 months field study by interviewing local informants from several villages. plant species used to treat infections were tested for antimicrobial activity. information was collected for 46 plant species from 30 families and for 5 animal species. twenty four of the plant species were cultivated. most used families were asteraceae, cucurbitaceae, lamiaceae and rosaceae and the most used plants were artemisia absi ...200415507351
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