| identification and characterization of klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol. | klebsiella pneumoniae produces 3-hydroxypropionic acid (3-hp) from glycerol with oxidation of 3-hydroxypropionaldehyde (3-hpa) to 3-hp in a reaction catalyzed by aldehyde dehydrogenase (aldh). in the present study, two putative aldhs of k. pneumoniae, ynei and ydcw were identified and characterized. recombinant ynei was specifically active on 3-hpa and preferred nad(+) as a cofactor, whereas ydcw exhibited broad substrate specificity and preferred nadp(+) as a cofactor. overexpression of aldhs i ... | 2013 | 23297067 |
| the role of aldehyde/alcohol dehydrogenase (adhe) in ethanol production from glycerol by klebsiella pneumoniae. | transcriptome analysis of a k. pneumoniae gem167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed adhe at a high level. ethanol production decreased significantly, from 8.8 to 0.5 g l(-1), when an adhe-deficient derivative of that strain was grown on glycerol. bacterial growth was also reduced under such conditions, showing that adhe plays a critical role in maintenance of redox balance by catal ... | 2013 | 23296976 |
| complete sequence of a bla(kpc-2)-harboring incfii(k1) plasmid from a klebsiella pneumoniae sequence type 258 strain. | we report the nucleotide sequence of a novel bla(kpc-2)-harboring incfii(k1) plasmid, pbk32179, isolated from a carbapenem-resistant klebsiella pneumoniae st258 strain from a new york city patient. pbk32179 is 165 kb long, consists of a large backbone of pkpn3-like plasmid, and carries an 18.5-kb bla(kpc-2)-containing element that is highly similar to plasmid pkpqil. pbk32179-like plasmids were identified in 8.3% of strains in a collection of 96 k. pneumoniae isolates from hospitals in the new y ... | 2013 | 23295924 |
| population-based incidence of carbapenem-resistant klebsiella pneumoniae along the continuum of care, los angeles county. | carbapenem-resistant klebsiella pneumoniae (crkp) is an emerging multidrug-resistant pathogen associated with higher mortality, longer hospital stays, and increased costs. crkp was thought to be sporadic in los angeles county (lac); however, the actual incidence is unknown. to address this, lac declared crkp a laboratory-reportable disease on june 1, 2010. | 2013 | 23295560 |
| emphysematous liver abscesses complicated by septic pulmonary emboli in patients with diabetes: two cases. | an emphysematous liver abscess is a fatal condition that often occurs in patients with uncontrolled diabetes mellitus. i herein describe two cases of klebsiella pneumoniae-induced emphysematous liver abscesses complicated by septic pulmonary emboli in patients with poorly controlled diabetes mellitus. both patients showed hemoglobin a1c levels of more than 10% and did not present with any abdominal symptoms on admission. however, they were diagnosed and successfully treated with percutaneous tra ... | 2013 | 23291690 |
| evaluation of three selective chromogenic media, chromagar esbl, chromagar ctx-m and chromagar kpc, for the detection of klebsiella pneumoniae producing oxa-48 carbapenemase. | three selective chromogenic culture media (chromagars esbl, ctx-m and kpc) were evaluated for their ability to support the growth of nine klebsiella pneumoniae isolates producing oxa-48 carbapenemase in combination with other β-lactamases. chromagar esbl and chromagar kpc were the most sensitive media, supporting growth of all isolates with a detection limit as low as < 100 cfu/ml. five isolates failed to grow on chromagar ctx-m, and five were recovered on chromagar kpc only at counts > 10(6) cf ... | 2013 | 23288920 |
| long-term faecal carriage in infants and intra-household transmission of ctx-m-15-producing klebsiella pneumoniae following a nosocomial outbreak. | to investigate the duration of faecal carriage of ctx-m-15-producing klebsiella pneumoniae in infants colonized during a nosocomial neonatal intensive care unit (nicu) outbreak after discharge from hospital, possible risk factors for long-term colonization and transmission to household contacts (hcs). | 2013 | 23288401 |
| [rectal surveillance of carbapenemases in klebsiella pneumoniae: comparison of pcr and culture]. | | 2012 | 23282509 |
| first report of a klebsiella pneumoniae strain coproducing ndm-1, vim-1 and oxa-48 carbapenemases isolated in morocco. | | 2013 | 23278254 |
| complete sequence of an incn plasmid, pimp-hz1, carrying blaimp-4 in a klebsiella pneumoniae strain associated with medical travel to china. | | 2013 | 23274671 |
| resveratrol suppresses calcium-mediated microglial activation and rescues hippocampal neurons of adult rats following acute bacterial meningitis. | acute bacterial meningitis (abm) is a serious disease with severe neurological sequelae. the intense calcium-mediated microglial activation and subsequently pro-inflammatory cytokine release plays an important role in eliciting abm-related oxidative damage. considering resveratrol possesses significant anti-inflammatory and anti-oxidative properties, the present study aims to determine whether resveratrol would exert beneficial effects on hippocampal neurons following abm. abm was induced by ino ... | 2013 | 23273676 |
| characterization of klebsiella pneumoniae strains isolated from urinary tract infections: detection of esbl characteristics, antibiotic susceptibility and rapd genotyping. | in this study, a hundred klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (esbl) production. the random amplified polymorphic dna (rapd-pcr) method was used to identify the genetic differentiation of k. pneumoniae isolates. a total of 26 different dna bands ranging between 334 bp and 28033 bp were detected among the strains. it was found that 100 k ... | 2013 | 24730135 |
| colonization of peripheral intravascular catheters with biofilm producing microbes: evaluation of risk factors. | biofilms often colonize catheters and contribute to catheter-related septicemia. however, predictors of catheter colonization by biofilms remain poorly defined. the aim of this study was to evaluate clinical factors that may be associated with biofilm colonization of catheters. | 2012 | 23271843 |
| risk factors for bacteriuria with carbapenem-resistant klebsiella pneumoniae and its impact on mortality: a case-control study. | the objective of this study was to evaluate the mortality of and risk factors for bacteriuria due to carbapenem-resistant klebsiella pneumoniae (crkp) versus carbapenem-susceptible k. pneumoniae (cskp) producing extended spectrum β lactamase (esbl). | 2013 | 23271210 |
| chemical and antimicrobial studies on the essential oil from salvia santolinifolia boiss. | in view of the reputation of genus salvia in folklore medicine and its abundance in our region, the chemical composition and antimicrobial activity of essential oil from s. santolinifolia boiss. was analyzed. chemical analysis, using gas chromatography and gas chromatography mass spectrometry, retention indices and c-13 nuclear magnetic resonance spectroscopy has resulted in identification of 116 constituents, comprising about 97% of the total constituents. out of these 116, 78 constituents are ... | 2013 | 23261726 |
| current efficacy of antibiotics against klebsiella isolates from urine samples - a multi-centric experience in karachi. | due to emergence of bacterial resistant strains, the effectiveness of current antibiotic treatment without culture/sensitivity testing is questionable. our study aims to assess the present sensitivity profiles of klebsiella isolates from urine samples and provide options for empiric prescription in critically ill patients. klebsiella pneumoniae isolates collected over a period of 28 months till january 2011 from 1,617 urine samples of subjects presenting with urinary tract infections were identi ... | 2013 | 23261722 |
| rhamnolipid (rl) from pseudomonas aeruginosa obp1: a novel chemotaxis and antibacterial agent. | in the present study, the interaction of rhamnolipid produced by pseudomonas aeruginosa obp1 with the cell surfaces of staphylococcus aureus (mtcc 3160) and klebsiella pneumoniae (mtcc 618) were studied. rhamnolipid concentration below critical micelle concentration (cmc) did not exhibit significant antibacterial activity. however, on increasing rhamnolipid concentration beyond cmc a prominent antibacterial activity was observed. the results demonstrated different degree of rhamnolipid interacti ... | 2013 | 23261573 |
| klebsiella pneumoniae antimicrobial drug resistance, united states, 1998-2010. | we studied antimicrobial-resistant klebsiella pneumoniae for 1998-2010 by using data from the surveillance network. susceptibility results (n = 3,132,354) demonstrated significant increases in resistance to all antimicrobial drugs studied, except tetracycline. cross-resistance among carbapenem-resistant k. pneumoniae was lower for tetracycline and amikacin. | 2013 | 23260464 |
| epidemic of klebsiella pneumoniae st11 clone coproducing kpc-2 and 16s rrna methylase rmtb in a chinese university hospital. | emergence of rmtb-positive klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae (kpc-kp) poses a great threat to antimicrobial treatment options. | 2012 | 23259910 |
| structural and mechanistic studies of hpxo, a novel flavin adenine dinucleotide-dependent urate oxidase from klebsiella pneumoniae. | hpxo is a flavin-dependent urate oxidase that catalyzes the hydroxylation of uric acid to 5-hydroxyisourate and functions in a novel pathway for purine catabolism found in klebsiella pneumoniae. we have determined the structures of hpxo with and without uric acid at 2.0 and 2.2 å, respectively. we have also determined the structure of the r204q variant at 2.0 å resolution in the absence of uric acid. the variant structure is very similar to that of wild-type hpxo except for the conformation of a ... | 2013 | 23259842 |
| kpc presence in pseudomonas aeruginosa has minimal impact on the in vivo efficacy of carbapenem therapy. | while reports of klebsiella pneumoniae carbapenemase (kpc) production among pseudomonas aeruginosa strains have emerged from a number of countries worldwide, outcome data are lacking. this is the first report evaluating how kpc production in p. aeruginosa impacts the efficacy of carbapenems by using the murine thigh infection model. our findings suggest that the impact of kpc-2 in vivo is less pronounced than would be anticipated based on the in vitro potency. | 2013 | 23254422 |
| bloodstream infections caused by carbapenemase-producing klebsiella pneumoniae: a clinical perspective. | dissemination of carbapenemase-producing klebsiella pneumoniae (cp-kp) has caused a public health crisis that can be paralleled with that caused by the spread of mrsa. cp-kps, being multidrug-resistant, mainly affect patients with severe underlying conditions in the acute-healthcare setting. cp-kps are responsible for a variety of life-threatening infections including bacteremia and pneumonia. the shortage of therapeutic options has forced clinicians to use colistin as well as tigecycline, a nov ... | 2012 | 23253318 |
| design and exploration of novel boronic acid inhibitors reveals important interactions with a clavulanic acid-resistant sulfhydryl-variable (shv) β-lactamase. | inhibitor resistant (ir) class a β-lactamases pose a significant threat to many current antibiotic combinations. the k234r substitution in the shv β-lactamase, from klebsiella pneumoniae , results in resistance to ampicillin/clavulanate. after site-saturation mutagenesis of lys-234 in shv, microbiological and biochemical characterization of the resulting β-lactamases revealed that only -arg conferred resistance to ampicillin/clavulanate. x-ray crystallography revealed two conformations of arg-23 ... | 2013 | 23252553 |
| assessment of antimicrobial combinations for klebsiella pneumoniae carbapenemase-producing k. pneumoniae. | the prevalence of bla(kpc) among gram-negative bacteria continues to increase worldwide. limited treatment options exist for this multidrug-resistant phenotype, often necessitating combination therapy. we investigated the in vitro and in vivo efficacy of multiple antimicrobial combinations. | 2013 | 23242537 |
| continuous surveillance to reduce extended-spectrum β-lactamase klebsiella pneumoniae colonization in the neonatal intensive care unit. | clinical illness caused by resistant bacteria usually represents a wider problem of asymptomatic colonization. active surveillance with appropriate institution of isolation precautions represents a potential mechanism to control colonization and reduce infection. the neonatal intensive care unit (nicu) is an environment particularly appropriate for such interventions. neonates are rarely colonized by resistant bacteria on admission and staff enthusiasm for infection control is high. | 2013 | 23235260 |
| emergence of klebsiella pneumoniae coharboring kpc and vim carbapenemases in colombia. | | 2013 | 23229478 |
| plasmid content of a clinically relevant klebsiella pneumoniae clone from the czech republic producing ctx-m-15 and qnrb1. | the entire plasmid content of a multidrug-resistant, ctx-m-15-producing klebsiella pneumoniae st416 clone was investigated. two fii(k) plasmids, pkdo1 (127 kb) and pkpn-cz (207 kb), were identified and found to carry a formidable set of genes conferring resistance to toxic compounds, metals, and antimicrobial drugs and exhibiting novel features putatively associated with adaptation and fitness of the bacterium in the human host. | 2013 | 23229477 |
| simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of the lactate dehydrogenase-deficient recombinant klebsiella pneumoniae overexpressing an aldehyde dehydrogenase. | in the present study, the lactate dehydrogenase-deficient (ldha(-)) recombinant klebsiella pneumoniae overexpressing an aldh (kgsadh) was developed and the co-production of 3-hp and pdo from glycerol by this recombinant under resting cell conditions was examined. the new recombinant did not produce any appreciable lactate, which seriously inhibits the production of 3-hp and pdo. the final titers of 3-hp and pdo by the ldha(-) recombinant strain at 60 h were 252.2 mm and 308.7 mm, respectively, w ... | 2013 | 23228456 |
| metabolic response to klebsiella pneumoniae infection in an experimental rat model. | bacteremia, the presence of viable bacteria in the blood stream, is often associated with several clinical conditions. bacteremia can lead to multiple organ failure if managed incorrectly, which makes providing suitable nutritional support vital for reducing bacteremia-associated mortality. in order to provide such information, we investigated the metabolic consequences of a klebsiella pneumoniae (k. pneumoniae) infection in vivo by employing a combination of (1)h nuclear magnetic resonance spec ... | 2012 | 23226457 |
| survey of klebsiella pneumoniae bacteraemia in two south australian hospitals and detection of hypermucoviscous phenotype and maga/rmpa genotypes in k . pneumoniae isolates. | clinical patterns of klebsiella pneumoniae bacteraemia vary geographically. an invasive syndrome involving abscess formation has emerged in recent years. putative virulence factors associated with this syndrome include colony hypermucoviscosity, and maga and rmpa genes. we studied epidemiologic and microbiologic characteristics of k. pneumoniae bacteraemia at two south australian hospitals and identified cases of k. pneumoniae invasive syndrome. we determined the frequency of the hypermucoviscos ... | 2013 | 23225226 |
| anatomic sites of patient colonization and environmental contamination with klebsiella pneumoniae carbapenemase-producing enterobacteriaceae at long-term acute care hospitals. | to determine anatomic sites of colonization in patients and to assess environmental contamination with klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae. | 2013 | 23221193 |
| nosocomial transmission of new delhi metallo-β-lactamase-1-producing klebsiella pneumoniae in toronto, canada. | an analysis of a cluster of new delhi metallo-β-lactamase-1-producing klebsiella pneumoniae (ndm1-kp) and a retrospective case-cohort analysis of risk factors for acquisition in contacts of ndm1-kp-positive patients. | 2013 | 23221192 |
| virulence profiles and antibiotic susceptibility patterns of klebsiella pneumoniae strains isolated from different clinical specimens. | to detect virulence factors in 54 klebsiella pneumoniae isolates from different clinical specimens: urine (26), blood (11), pus (11), lung (four), cerebrospinal fluid (one) and ascitic fluid (one). | 2013 | 23218835 |
| [new description of a ndm-1 carbapenemase producing klebsiella pneumoniae carrier in spain]. | | 2013 | 23218738 |
| characterization of klebsiella pneumoniae strains producing extended spectrum beta-lactamases and ampc type beta-lactamases isolated from hospitalized patients in kerman, iran. | klebsiella pneumoniae is a major cause of nosocomial infections. emergence of antibacterial resistance and production of beta-lactamases are responsible for the frequently observed empirical therapy failures. the aim of this study was to determine the presence and the prevalence of extended spectrum beta-lactamases (esbls) and ampc beta-lactamases in clinical isolates of k. pneumoniae in kerman, iran. resistance to different antibiotics was determined using standard disk diffusion method. the be ... | 2012 | 23210321 |
| genome sequence of oxa-48 carbapenemase-producing klebsiella pneumoniae kpo3210. | klebsiella pneumoniae kpo3210 is a oxa-48 carbapenemase-producing isolate obtained from a blood culture in the context of an outbreak in hospital universitario la paz (madrid, spain) in 2010. it belongs to the major clone detected during the outbreak and is resistant to all beta-lactams and to several other antibiotics. | 2012 | 23209233 |
| clpp-dependent and -independent activities encoded by the polycistronic clpk-encoding locus contribute to heat shock survival in klebsiella pneumoniae. | the family of clp atpases plays an important role in bacterial physiology. here we characterize the genetic locus encompassing a newly described plasmid-encoded clpk protein protecting klebsiella pneumoniae cells during heat shock. we demonstrate that the clpk gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. clpk is encoded by a multifunctional transcriptional unit characterized by both clpp-d ... | 2013 | 23201206 |
| multiple-omic data analysis of klebsiella pneumoniae mgh 78578 reveals its transcriptional architecture and regulatory features. | the increasing number of infections caused by strains of klebsiella pneumoniae that are resistant to multiple antibiotics has developed into a major medical problem worldwide. the development of next-generation sequencing technologies now permits rapid sequencing of many k. pneumoniae isolates, but sequence information alone does not provide important structural and operational information for its genome. | 2012 | 23194155 |
| engineered constitutive pathway in klebsiella pneumoniae for 3-hydroxypropionic acid production and implications for decoupling glycerol dissimilation pathways. | 3-hydroxypropionic acid (3-hp) is a commercially important platform chemical from which a panel of chemicals can be generated. klebsiella pneumoniae has been regarded as a promising host strain in glycerol-based 3-hp production for its exceptional ability to metabolize glycerol. since the glycerol dissimilation mechanism governs the carbon flux distribution from glycerol, inducible strong promoters were usually employed to enhance the glycerol consumption and 3-hp production. here, we report an ... | 2013 | 23192305 |
| [comparison of ertapenem-emb agar with traditional methods for screening carbapenem-resistant klebsiella pneumoniae from rectal swabs]. | detection of rectal colonization with carbapenem-resistant klebsiella pneumoniae (crkp) is the most important step in the infection control protocols in order to prevent infections caused by crkp which has an increasing incidence all over the world. in this study, it was aimed to compare the detection rate of 2 mg/l ertapenem emb agar medium with the other methods recommended by various international guidelines. these methods include direct plate method using ertapenem disc, enrichment method in ... | 2012 | 23188568 |
| first detection of the ampc beta-lactamase acc-1 in a klebsiella pneumoniae isolate in bulgaria. | | 2012 | 23182053 |
| bactericidal activity of five antiseptics on klebsiella pneumoniae and its relationship to the presence of efflux pump genes and influence of organic matter. | | 2012 | 23182050 |
| kinetic modeling of fermentative production of 1, 3-propanediol by klebsiella pneumoniae hr526 with consideration of multiple product inhibitions. | during the fermentative production of 1, 3-propanediol (1,3-pd), the multiple product inhibitions cannot be negligible to accurately describe the kinetics of fermentation process. a kinetic model for fermentative production of 1,3-pd by klebsiella pneumoniae hr526 with glycerol as carbon source under aerobic condition was proposed. the inhibitions of multiple products including 1,3-pd, 2, 3-butanediol (2,3-bd), acetate, and succinate were considered in the model. it was found that 1,3-pd, 2,3-bd ... | 2013 | 23179286 |
| early identification and control of carbapenemase-producing klebsiella pneumoniae, originating from contaminated endoscopic equipment. | klebsiella producing carbapenemase is an emerging pathogen. we report transmission of this organism by contaminated endoscopic instruments. quick identification of source, staff education, contact precautions, and emphasis on hand and environmental hygiene led to case control and prevention of outbreak. | 2013 | 23171594 |
| interaction of lipocalin 2, transferrin, and siderophores determines the replicative niche of klebsiella pneumoniae during pneumonia. | pathogenic bacteria require iron for replication within their host. klebsiella pneumoniae and other gram-negative pathogens produce the prototypical siderophore enterobactin (ent) to scavenge iron in vivo. in response, mucosal surfaces secrete lipocalin 2 (lcn2), an innate immune protein that binds ent to disrupt bacterial iron acquisition and promote acute inflammation during colonization. a subset of k. pneumoniae isolates attempt to evade lcn2 by producing glycosylated ent (gly-ent, salmochel ... | 2012 | 23169997 |
| susceptibility of different phases of biofilm of klebsiella pneumoniae to three different antibiotics. | the existence of majority of bacteria in biofilm mode makes it difficult to eradicate them as antibiotics at much higher concentrations than the mics are required to destroy these bacteria. this study investigated the effect of different classes of antibiotics on different phases of biofilm formed by klebsiella pneumoniae. the organism was grown in different phases relevant to biofilm formation: planktonic cells at mid-log phase, planktonic cells at stationary phase, adherent monolayers and matu ... | 2013 | 23168403 |
| complete genome sequence of klebsiella pneumoniae phage jd001. | klebsiella pneumoniae is a member of the family enterobacteriaceae, opportunistic pathogens that are among the eight most prevalent infectious agents in hospitals. the emergence of multidrug-resistant strains of k. pneumoniae has became a public health problem globally. to develop an effective antimicrobial agent, we isolated a bacteriophage, named jd001, from seawater and sequenced its genome. comparative genome analysis of phage jd001 with other k. pneumoniae bacteriophages revealed that phage ... | 2012 | 23166250 |
| [primary causative agents of infectious complications in injured with severe trauma]. | study etiologic structure of infectious complications in injured with severe-trauma. | 2012 | 23163030 |
| blood stream infections (bsi) in severe burn patients--early and late bsi: a 9-year study. | bloodstream infections (bsi) and sepsis are among the most common complications occurring in severe burn patients. this study was designed to evaluate changes in bsi pathogens over almost a decade in severe burn patients at rambam healthcare campus, and bsi occurrence during early and late hospitalization periods. retrospective computerized data was retrieved from all severe burn patients hospitalized in our institution during the years 2001-2009. bsi in the first week was defined as early bsi, ... | 2013 | 23159703 |
| emergence of klebsiella pneumoniae-producing kpc-2 carbapenemase in paraíba, northeastern brazil. | the emergence of kpc-2 producing k. pneumoniae in hospitalized patients at the intensive care unit (icu) of a teaching hospital located in the city of joão pessoa, paraíba, brazil, is reported. seven carbapenem-resistant k. pneumoniae recovered from different body sites of infection were analyzed. most isolates showed a multidrug-resistance phenotype. genotypic analysis demonstrated the presence of two genotypes, with the predominance of genotype a, which belongs to st 437. these isolates also c ... | 2013 | 23158264 |
| carbapenem-sparing antibiotic regimens for infections caused by klebsiella pneumoniae carbapenemase-producing k. pneumoniae in intensive care unit. | a carbapenem-sparing regimen of tigecycline plus gentamicin or colistin was effective for treating 24 of 26 (92%) klebsiella pneumoniae carbapenemase-producing k. pneumoniae infectious episodes in 22 polytrauma intensive care unit patients without comorbidities. the 30-day crude mortality rate was 14%. regimens were considered appropriate in 12% of episodes according to the vitek 2 system and in 100% based on e-test. | 2013 | 23155147 |
| standardized outpatient management of klebsiella pneumoniae liver abscesses. | community-acquired klebsiella pneumoniae has emerged as a major cause of liver abscess in asia. using a standardized protocol, we conducted a prospective cohort study of all cases of k. pneumoniae liver abscess treated from 2005 to 2011 at two outpatient parenteral antimicrobial therapy (opat) centers in singapore, to assess the safety and efficacy of treatment. | 2013 | 23154175 |
| carbapenem-resistant klebsiella pneumoniae harbouring blakpc-3 and blavim-2 from central italy. | the frequency of klebsiella pneumoniae carbapenemase-producing k. pneumoniae is increasing in italian hospitals and poses an emerging threat to the management of infections in hospitalized patients. in this study, we report a detailed molecular characterization of a k. pneumoniae subsp. pneumoniae kp1/11 isolate from the decubitus ulcer of a hospitalized patient with a serious infection. k. pneumoniae kp1/11 produces kpc-3 and vim-2 β-lactamases. the bla(kpc-3) gene is harbored in a large plasmi ... | 2013 | 23153971 |
| multidrug resistance genes, including bla(kpc) and bla(ctx)-m-2, among klebsiella pneumoniae isolated in recife, brazil. | the prevalence of cephalosporins and carbapenem-resistant klebsiella pneumoniae strains is rising in brazil, with potential serious consequences in terms of patients' outcomes and general care. | 2012 | 23152339 |
| ccaat-enhancer binding protein delta (c/ebpδ) protects against klebsiella pneumoniae-induced pulmonary infection: potential role for macrophage migration. | mounting evidence suggests an important role for ccaat-enhancer binding protein delta (c/ebpδ) in the acute-phase response after bacterial infection. however, whether c/ebpδ limits pneumonia remains elusive and is the aim of this study. therefore, bacterial outgrowth, inflammatory responses, inflammatory cell influx, and survival were assessed in wild-type and c/ebpδ(-/-) mice infected with klebsiella pneumoniae via the airways. we showed that c/ebpδ expression is highly induced in the lung duri ... | 2012 | 23148291 |
| [clinical features and antimicrobial resistance of community-acquired pneumonia caused by klebsiella pneumoniae in infants]. | to study the clinical features and antimicrobial resistance of community-acquired pneumonia caused by klebsiella pneumoniae in infants. | 2012 | 23146728 |
| transfer from high-acuity long-term care facilities is associated with carriage of klebsiella pneumoniae carbapenemase-producing enterobacteriaceae: a multihospital study. | to determine whether transfer from a long-term care facility (ltcf) is a risk factor for colonization with klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae upon acute care hospital admission. | 2012 | 23143355 |
| high rate of colistin resistance among patients with carbapenem-resistant klebsiella pneumoniae infection accounts for an excess of mortality. | carbapenem-resistant klebsiella pneumoniae (cr-kp) is becoming a common cause of healthcare-associated infection in italy, with high morbidity and mortality. prevalent cr-kp clones and resistance mechanisms vary between regions and over time. therapeutic approaches and their impact on mortality have to be investigated. we performed a prospective study of patients with cr-kp isolation, hospitalized in nine hospitals of rome, italy, from december 2010 to may 2011, to describe the molecular epidemi ... | 2013 | 23137235 |
| the therapeutic effect of tigecycline, unlike that of ceftazidime, is not influenced by whether the klebsiella pneumoniae strain produces extended-spectrum β-lactamases in experimental pneumonia in rats. | the efficacies of tigecycline and ceftazidime against fatal pneumonia in rats caused by an extended-spectrum β-lactamase (esbl)-positive klebsiella pneumoniae strain or its wild-type (wt) progenitor were compared. ceftazidime at 12.5 or 50 mg/kg of body weight twice daily (b.i.d.) was effective (50% or 100% rat survival) in pneumonia caused by the wt isolate but unsuccessful (100% rat mortality) in pneumonia caused by the esbl-positive variant. in contrast, tigecycline at 6.25, 12.5, or 25 mg/kg ... | 2013 | 23129049 |
| repression of oxalic acid-mediated mineral phosphate solubilization in rhizospheric isolates of klebsiella pneumoniae by succinate. | two strains of klebsiella (sm6 and sm11) were isolated from rhizospheric soil that solubilized mineral phosphate by secretion of oxalic acid from glucose. activities of enzymes for periplasmic glucose oxidation (glucose dehydrogenase) and glyoxylate shunt (isocitrate lyase and glyoxylate oxidase) responsible for oxalic acid production were estimated. in presence of succinate, phosphate solubilization was completely inhibited, and the enzymes glucose dehydrogenase and glyoxylate oxidase were repr ... | 2013 | 23124768 |
| amino acid substitutions of maga in klebsiella pneumoniae affect the biosynthesis of the capsular polysaccharide. | mucoviscosity-associated gene a (maga) of klebsiella pneumoniae contributes to k1 capsular polysaccharide (cps) biosynthesis. based on sequence homology and gene alignment, the maga gene has been predicted to encode a wzy-type cps polymerase. sequence alignment with the wzy_c and rfal protein families (which catalyze cps or lipopolysaccharide (lps) biosynthesis) and topological analysis has suggested that eight highly conserved residues, including g308, g310, g334, g337, r290, p305, h323, and n3 ... | 2012 | 23118860 |
| complete nucleotide sequences of blakpc-4- and blakpc-5-harboring incn and incx plasmids from klebsiella pneumoniae strains isolated in new jersey. | klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae have emerged as major nosocomial pathogens. bla(kpc), commonly located on tn4401, is found in gram-negative bacterial strains, with the two most common variants, bla(kpc-2) and bla(kpc-3), identified in plasmids with diverse genetic backgrounds. in this study, we examined bla(kpc-4)- and bla(kpc-5)-bearing plasmids recovered from two k. pneumoniae strains, which were isolated from a single new jersey hospital in 2005 and 2006 ... | 2013 | 23114770 |
| detection of extended spectrum b-lactamases in urinary isolates of klebsiella pneumoniae in relation to bla, bla and bla gene carriage. | resistance to contemporary broad-spectrum β-lactam antibiotics mediated by extended-spectrum β-lactamases (esbls) is increasing worldwide. klebsiella pneumoniae, an important cause of nosocomial and community acquired urinary tract infections has rapidly become the most common esbl producing organism. we examined esbl production in urinary isolates of k. pneumoniae in relation to the presence of bla(shv), bla(tem) and bla(ctx-m) genes. | 2012 | 23113157 |
| multiple cutaneous neutropenic ulcers associated with azathioprine. | we report a case of neutropenic ulceration in a 42-year-old woman receiving azathioprine for pemphigus vulgaris. she developed multiple indolent ulcers involving the nose, neck, and back, after about 6-8 weeks following commencement of azathioprine 50 mg daily. the ulcers were large, disfiguring, dry, and with basal necrotic slough. they were painless and did not discharge pus. the absolute neutrophil count was severely depressed initially, but normalized following azathioprine withdrawal. swab ... | 2015 | 23112431 |
| complete genome sequence of klebsiella pneumoniae 1084, a hypermucoviscosity-negative k1 clinical strain. | we report the complete genome sequence of klebsiella pneumoniae 1084, a hypermucoviscosity-negative k1 clinical strain. sequencing and annotation revealed a 5,386,705-bp circular chromosome (57.4% g+c content), which contains 4,962 protein-coding genes, 80 trna genes, and 25 rrna genes. | 2012 | 23105059 |
| klebsiella pneumoniae liver abscess: a new invasive syndrome. | klebsiella pneumoniae is a well known human nosocomial pathogen. most community-acquired k pneumoniae infections cause pneumonia or urinary tract infections. during the past two decades, however, a distinct invasive syndrome that causes liver abscesses has been increasingly reported in asia, and this syndrome is emerging as a global disease. in this review, we summarise the clinical presentation and management as well the microbiological aspects of this invasive disease. diabetes mellitus and tw ... | 2012 | 23099082 |
| interaction of dihydrofolate reductase and aminoglycoside adenyltransferase enzyme from klebsiella pneumoniae multidrug resistant strain df12sa with clindamycin: a molecular modelling and docking study. | klebsiella pneumoniae strain df12sa (hq114261) was isolated from diabetic foot wounds. the strain showed resistance against ampicillin, kanamycin, gentamicin, streptomycin, spectinomycin, trimethoprim, tetracycline, meropenem, amikacin, piperacillin/tazobactam, augmentin, co-trimoxazole, carbapenems, penicillins and cefoperazone, and was sensitive to clindamycin. molecular characterization of the multidrug-resistance phenotype revealed the presence of a class 1 integron containing two genes, a d ... | 2013 | 23097003 |
| [experience with tigecycline compassionate use in pediatric patients infected with carbapenem resistant klebsiella pneumoniae]. | infections produced by multidrug-resistant pathogens represent a therapeutic challenge because of the few therapeutic options available. tigecycline is a relatively new antibiotic, with a wide spectrum of activity including some of these resistant bacteria. in adults is prescribed for the treatment of some infections caused by carbapenem resistant k. pneumoniae, however it has not been approved in children because of potential adverse effects in the dental enamel. | 2012 | 23096472 |
| role of edta and cse1034 in curli formation and biofilm eradication of klebsiella pneumoniae: a comparison with other drugs. | | 2012 | 23093035 |
| prevalence and genotypic analysis of plasmid-mediated β-lactamases among urinary klebsiella pneumoniae isolates in moroccan community. | the aim of this study is to assess the prevalence and molecular characterization of the extended spectrum β-lactamases (esbl)-producing klebsiella pneumoniae isolated from community acquired urinary tract infections and collected in five moroccan cities during a 2010 survey. in all, 34 (7.5%) of the 453 k. pneumoniae isolates studied were positive for an esbl phenotype and 91.1% of these isolates were multidrug resistant. the bla(ctx-m-15) (n=31) was the most frequent esbl genes detected, follow ... | 2013 | 23093031 |
| comparative evaluation of pumice stone as an alternative immobilization material for 1,3-propanediol production from waste glycerol by immobilized klebsiella pneumoniae. | in this study, pumice stone (ps), which is a vastly available material in turkey, was evaluated as an alternative immobilization material in comparison to other commercially available immobilization materials such as glass beads and polyurethane foam. all immobilized bioreactors resulted in much better 1,3-propanediol production from waste glycerol in comparison to the suspended cell culture bioreactor. it was also demonstrated that the locally available ps material is as good as the commerciall ... | 2012 | 23079889 |
| phenotypic and molecular characterization of ctx-m-14 extended-spectrum β-lactamase and plasmid-mediated act-like ampc β-lactamase produced by klebsiella pneumoniae isolates from chickens in henan province, china. | extended-spectrum β-lactamases (esbls) and ampc β-lactamases produced by a clinical isolate of klebsiella pneumoniae from chickens were detected with confirmatory phenotypic tests of the clinical and laboratory standards institute. the minimum inhibitory concentrations of 18 antibacterial drugs against k. pneumoniae were determined by the 2-fold microdilution method. the genotype and subtype of the esbl-producing and ampc β-lactamase-producing k. pneumoniae isolate were identified by pcr amplifi ... | 2012 | 23079829 |
| update of the molecular epidemiology of kpc-2-producing klebsiella pneumoniae in brazil: spread of clonal complex 11 (st11, st437 and st340). | to perform molecular epidemiology for 113 kpc-producing klebsiella pneumoniae isolated in 2010 from 12 brazilian states. | 2013 | 23070735 |
| patient risk factors for outer membrane permeability and kpc-producing carbapenem-resistant klebsiella pneumoniae isolation: results of a double case-control study. | in the 1,200-bed university hospital "umberto i" in rome, italy, we observed a dramatic substitution of a precedingly well-documented klebsiella pneumoniae clone (st37) with ertapenem resistance by outer membrane permeability modification (porin-er-kp) with a new k. pneumoniae strain expressing carbapenem resistance due to k. pneumoniae carbapenemase production (kpc-cr-kp). a case-case-control study was carried out to evaluate risk factors for porin-er-kp and kpc-cr-kp isolation. | 2013 | 23070604 |
| surveillance and molecular epidemiology of klebsiella pneumoniae isolates that produce carbapenemases: first report of oxa-48-like enzymes in north america. | a study was designed to characterize nonrepeat isolates of carbapenemase-producing k. pneumoniae obtained from the smart worldwide surveillance program during 2008 and 2009. characterization was done by pcr and sequencing for bla(vim), bla(imp), bla(ndm), bla(oxa), bla(kpc), and plasmid-mediated quinolone resistance and virulence factors (vfs). genetic relatedness was determined with pulsed-field gel electrophoresis (pfge) using xbai and multilocus sequence typing. a total of 110 isolates were i ... | 2013 | 23070171 |
| differential microrna (mirna) expression could explain microbial tolerance in a novel chronic peritonitis model. | we observed persistent peritoneal bacteria despite a transient early innate immune response to intraperitoneal (ip) klebsiella pneumoniae. pretreatment with lps prior to peritonitis induced a tolerant pattern of pro-inflammatory cytokine protein production over 72 h, but not at the mrna level. micrornas (mirnas) regulate inflammatory cytokines and may explain this paradox. after pretreatment with ip lps or saline, c57bl/6 mice were given 10(3) cfu of k. pneumoniae ip. total rna was isolated from ... | 2013 | 23060456 |
| heterocyclic methylsulfone hydroxamic acid lpxc inhibitors as gram-negative antibacterial agents. | the synthesis and antibacterial activity of heterocyclic methylsulfone hydroxamates is presented. compounds in this series are potent inhibitors of the lpxc enzyme, a key enzyme involved in the production of lipopolysaccharide (lps) found in the outer membrane of gram-negative bacteria. sar evaluation of compounds in this series revealed analogs with potent antibacterial activity against challenging gram-negative species such as pseudomonas aeruginosa and klebsiella pneumoniae. | 2012 | 23046961 |
| draft genome sequence of an extremely drug-resistant kpc-producing klebsiella pneumoniae st258 epidemic strain. | extremely drug-resistant (xdr) klebsiella pneumoniae carbapenemase-producing clone st258 has rapidly disseminated worldwide. we report here the draft genome sequence of the k. pneumoniae st258 xdr clinical strain from israel. | 2012 | 23045514 |
| infections caused by oxa-48-producing klebsiella pneumoniae in a tertiary hospital in spain in the setting of a prolonged, hospital-wide outbreak. | we describe clinical and microbiological features of infections caused by oxa-48-producing klebsiella pneumoniae (o48kp) in the setting of a prolonged, hospital-wide outbreak detected in january 2011. | 2013 | 23045224 |
| endogeneous endophthalmitis by klebsiella pneumoniae complicated with corneal perforation in intensive care unit. | | 2013 | 23044741 |
| unique genes identified in the epidemic extremely drug-resistant kpc-producing klebsiella pneumoniae sequence type 258. | a kpc-producing klebsiella pneumoniae clone, sequence type (st) 258, has emerged and spread worldwide. this study aimed to identify putative genes that may contribute to the extraordinary dissemination of the kpc-producing st258 clone. | 2013 | 23042812 |
| first report of kpc-producing klebsiella pneumoniae in croatia. | in february 2011, a 78-year-old male patient was admitted to clinical hospital center zagreb with subdural haematoma. klebsiella pneumoniae with reduced susceptibility to carbapenems was isolated. pcr revealed the presence of bla(kpc), bla(tem), and bla(shv) genes. sequencing of bla(kpc) gene identified k. pneumoniae carbapenemase (kpc)-2 beta-lactamase. the strain belonged to st37 clone by multilocus sequence typing. infection control efforts limited the spread of kpc-producing clone of k. pneu ... | 2012 | 23040691 |
| emergence of oxa-48-producing klebsiella pneumoniae and the novel carbapenemases oxa-244 and oxa-245 in spain. | to describe the molecular and population-level characterization of a selected group of oxa-48-like-producing klebsiella pneumoniae isolates collected in spain between january 2011 and may 2012. | 2013 | 23034714 |
| the emergence of multidrug-resistant klebsiella pneumoniae of international clones st13, st16, st35, st48 and st101 in a teaching hospital in the paris region. | despite infection control measures, an important increase in the extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae incidence density occurred in our hospital from 2006 onwards. this study, focusing on the 2005-2007 period, was performed in an attempt to explain this increase. esbls were characterized, isolates were typed by eric2-pcr, and sequence type (st) of clustered isolates was determined. temporal-spatial relationships of patients were analysed to assess possible cross-c ... | 2013 | 23034125 |
| differences in characteristics between healthcare-associated and community-acquired infection in community-onset klebsiella pneumoniae bloodstream infection in korea. | healthcare-associated (hca) infection has emerged as a new epidemiological category. the aim of this study was to evaluate the impact of hca infection on mortality in community-onset klebsiella pneumoniae bloodstream infection (kpbsi). | 2012 | 23034099 |
| risk of infections subsequent to pyogenic liver abscess: a nationwide population-based study. | this nationwide study aimed to provide risk estimates for a panel of infections subsequent to pyogenic liver abscesses (pla) in taiwan. in this study, we selected 12 050 patients diagnosed with pla as our study cohort and 60 250 non-pla patients as our comparison cohort. we individually tracked each subject for a 1-year period beginning with their index date to identify those who were subsequently diagnosed with any of the following infections: pneumonia, endophthalmitis, septic pulmonary emboli ... | 2013 | 23034092 |
| immunity to the conserved influenza nucleoprotein reduces susceptibility to secondary bacterial infections. | influenza causes >250,000 deaths annually in the industrialized world, and bacterial infections frequently cause secondary illnesses during influenza outbreaks, including pneumonia, bronchitis, sinusitis, and otitis media. in this study, we demonstrate that cross-reactive immunity to mismatched influenza strains can reduce susceptibility to secondary bacterial infections, even though this fails to prevent influenza infection. specifically, infecting mice with h3n2 influenza before challenging wi ... | 2012 | 23028058 |
| effect of puuc overexpression and nitrate addition on glycerol metabolism and anaerobic 3-hydroxypropionic acid production in recombinant klebsiella pneumoniae δglpkδdhat. | 3-hydroxypropionic acid (3-hp), an industrially important platform chemical, is used as a precursor during the production of many commercially important chemicals. recently, recombinant strains of k. pneumoniae overexpressing an nad(+)-dependent γ-glutamyl-γ-aminobutyraldehyde dehydrogenase (puuc) enzyme of k. pneumoniae dsm 2026 were shown to produce 3-hp from glycerol without the addition coenzyme b(12), which is expensive. however, 3-hp production in k. pneumoniae is accompanied with nadh gen ... | 2013 | 23022570 |
| draft genome sequence of klebsiella pneumoniae subsp. pneumoniae dsm 30104t. | klebsiella pneumoniae is a gram-negative, rod-shaped, nonmotile, and opportunistic pathogenic species with clinical importance. it is a part of natural flora of humans and animals. here we report the draft genome sequence of the type strain of klebsiella pneumoniae subsp. pneumoniae (dsm 30104(t)) to provide taxonomic and functional insights into the species. | 2012 | 23012294 |
| contribution of oqxab efflux pumps to quinolone resistance in extended-spectrum-β-lactamase-producing klebsiella pneumoniae. | the aims of this study were to analyse the presence of oqxa and oqxb genes in a collection of extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae strains, to determine their chromosomal and/or plasmidic locations and to analyse expression levels in relation to susceptibility or resistance to quinolones. | 2013 | 23011289 |
| functional characterization of a novel mn2+ dependent protein serine/threonine kinase kpnk, produced by klebsiella pneumoniae strain mgh78578. | klebsiella pneumoniae mgh78578 contains ~500 uncharacterized signaling proteins and in this study, we characterized the biological functions of a novel eukaryotic-like serine/threonine kinase; estk (kpnk). studies demonstrated that kpnk undergoes autophosphorylation within the ph range 7.0-7.5 at 37°c in a time- and concentration- dependent manner, with mn(2+) as its cofactor. the δkpnk mutant exhibited higher sensitivity to gastrointestinal and oxidative stresses. deletion of kpnk resulted in a ... | 2012 | 23010593 |
| effects of ph and dissolved co2 level on simultaneous production of 2,3-butanediol and succinic acid using klebsiella pneumoniae. | the influences of ph and dissolved co2 level on the regulation of growth and formation of catabolic end products have been investigated in klebsiella pneumoniae. with increasing co2 levels, there were no apparent changes in 2,3-butanediol production but succinic acid productions were enhanced significantly. a novel strategy for co-production of 2,3-butanediol and succinic acid using k. pneumoniae was developed by controlling ph and dissolved co2 concentration in fermentation medium. under the op ... | 2013 | 23010216 |
| emergence of resistance to fluoroquinolones among gram positive and gram negative clinical isolates. | fluoroquinolones are broad-spectrum antibiotics that are considered as first line drugs to treat infectious diseases. in order to find out useful fluoroquinolones, the antibiotic resistance of fluoroquinolones, namely, ofloxacin (ofl), ciprofloxacin (cip), norfloxacin (nrf), enoxacin (enx), pefloxacin (pfl) and levofloxacin (lvf) was investigated against ninety five clinical isolates that includes staphylococcus aureus, pseudomonas aeruginosa, klebsiella pneumoniae and proteus mirabilis. in vitr ... | 2012 | 23010009 |
| molecular basis for the increased polymyxin susceptibility of klebsiella pneumoniae strains with under-acylated lipid a. | the impact of under-acylation of lipid a on the interaction between klebsiella pneumoniae lps and polymyxins b and e was examined with fluorometric and calorimetric methods, and by (1)h nmr, using a paired wild type (wt) and the δlpxm mutant strains b5055 and b5055δlpxm, which predominantly express lps with hexa- and penta-acylated lipid a structures respectively. lps from b5055δlpxm displayed a fourfold increased binding affinity for polymyxins b and e compared with the b5055 wt lps. ec50 value ... | 2013 | 23008349 |
| microbiological and clinical characteristics of bacteraemia caused by the hypermucoviscosity phenotype of klebsiella pneumoniae in korea. | | 2013 | 23006593 |
| molecular typing and resistance mechanisms of imipenem-non-susceptible klebsiella pneumoniae in taiwan: results from the taiwan surveillance of antibiotic resistance (tsar) study, 2002-2009. | we investigated the molecular mechanisms and clonality of imipenem-non-susceptible klebsiella pneumoniae isolates collected during a taiwan national surveillance programme, between 2002 and 2009. genes for carbapenemases, plasmid-borne ampc-type genes and extended-spectrum β-lactamase (esbl) genes were analysed by pcr. the major porin channels ompk35 and ompk36 were studied by sds-page. molecular typing was performed with pulsed-field gel electrophoresis (pfge) and multi-locus sequence typing (m ... | 2013 | 23002067 |
| adenosine a(2b) receptor deficiency promotes host defenses against gram-negative bacterial pneumonia. | activation of the adenosine a(2b) receptor (a(2b)r) promotes antiinflammatory effects in diverse biological settings, but the role of this receptor in antimicrobial host defense in the lung has not been established. gram-negative bacillary pneumonia is a common and serious illness associated with high morbidity and mortality, the treatment of which is complicated by increasing rates of antibiotic resistance. | 2012 | 22997203 |
| rapid low-cost detection of klebsiella pneumoniae carbapenemase genes by internally controlled real-time pcr. | an internally controlled real-time pcr assay based on sybr green was developed to screen klebsiella pneumoniae carbapenemases (kpcs) gene containing bacteria and was validated for clinical strains or surveillance specimens. when 248 clinical samples were tested, the sensitivity and specificity of the assay were 100% and 99%, respectively. | 2012 | 22995713 |
| strategies of empiric antibiotic therapy in severe sepsis. | severe sepsis and septic shock remain the leading causes of multiple organ failure and mortality in surgical intensive care units. early antibiotic therapy recently became a challenge, because of the increased number of infections caused by multiple drug resistant bacteria, with gram-negative bacteria such as klebsiella pneumoniae and pseudomonas aeruginosa being the most frequently cultured pathogens.in this review, detailed recommendations for the treatment of various infections are presented ... | 2012 | 22992970 |
| case of endogenous endophthalmitis caused by klebsiella pneumoniae with maga and rmpa genes in an immunocompetent patient. | we report a case of endogenous endophthalmitis caused by klebsiella pneumoniae in an immunocompetent patient. a 73-year-old man with acute epididymitis who had no history of diabetes mellitus developed endogenous endophthalmitis. the patient underwent anterior vitrectomy and intracapsular cataract extraction with intravitreal injections of both vancomycin and ceftazidime. after the surgery, he was treated with topical and intravenous antibiotics; however, the left eye perforated and was enucleat ... | 2013 | 22992835 |