| [the application of loop-mediated isothermal amplification for detection of common pathogenic bacteria in lower respiratory tract infections]. | to investigate the spectrum of common pathogenic bacteria of low respiratory tract infection by loop-mediated isothermal amplification (lamp) of nucleic acid test and to prove the clinical significance of this method. | 2014 | 24969715 |
| extended-spectrum beta-lactamase-producing klebsiella pneumoniae causing peritonitis in a patient on continuous ambulatory peritoneal dialysis. | | 2014 | 24969210 |
| outbreak of pseudomonas aeruginosa and klebsiella pneumoniae bloodstream infections at an outpatient chemotherapy center. | four patients were hospitalized july 2011 with pseudomonas aeruginosa bloodstream infection (bsi), 2 of whom also had klebsiella pneumoniae bsi. all 4 patients had an indwelling port and received infusion services at the same outpatient oncology center. | 2014 | 24969124 |
| [the spread of kpc-3 klebsiella pneumoniae in hospitals in bogotá over a three-year period (2008-2010)]. | introduction : one of the major worldwide public health problems today are the infections caused by carbapenem-resistant enterobacteriaceae (cre), among which carbapenem-resistant klebsiella pneumoniae (crkp), constitutes one of the most common pathogens causing nosocomial infection. | 2014 | 24968054 |
| [behavior of carbapenemase-producing klebsiella pneumoniae cases in cancer patients at a third level hospital in bogotá, d.c]. | the presence of carbapenemase-producing enterobacteriaceae in hospitals is increasingly common. patients with advanced cancer who require invasive means for diagnosis, treatment or palliative care, and the use of broad-spectrum antimicrobials to treat secondary infections show increased susceptibility to infections caused by these bacteria. | 2014 | 24968049 |
| aspergillomarasmine a overcomes metallo-β-lactamase antibiotic resistance. | the emergence and spread of carbapenem-resistant gram-negative pathogens is a global public health problem. the acquisition of metallo-β-lactamases (mbls) such as ndm-1 is a principle contributor to the emergence of carbapenem-resistant gram-negative pathogens that threatens the use of penicillin, cephalosporin and carbapenem antibiotics to treat infections. to date, a clinical inhibitor of mbls that could reverse resistance and re-sensitize resistant gram-negative pathogens to carbapenems has n ... | 2014 | 24965651 |
| epidemic klebsiella pneumoniae st258 is a hybrid strain. | carbapenem-resistant enterobacteriaceae (cre), especially klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae, pose an urgent threat in health facilities in the united states and worldwide. k. pneumoniae isolates classified as sequence type 258 (st258) by multilocus sequence typing are largely responsible for the global spread of kpc. a recent comparative genome study revealed that st258 k. pneumoniae strains are two distinct genetic clades; however, the molecular origin of st258 l ... | 2014 | 24961694 |
| utilization of excess nadh in 2,3-butanediol-deficient klebsiella pneumoniae for 1,3-propanediol production. | to utilize excess nadh for 1,3-propanediol production by 2,3-butanediol-deficient mutants, the effect of dhat overexpression in two distinct 2,3-butanediol-deficient mutants was investigated. | 2014 | 24961176 |
| carbapenemase-producing klebsiella pneumoniae and hematologic malignancies. | | 2014 | 24960464 |
| deaths attributable to carbapenem-resistant enterobacteriaceae infections. | we evaluated the number of deaths attributable to carbapenem-resistant enterobacteriaceae by using studies from around the world published before april 9, 2012. attributable death was defined as the difference in all-cause deaths between patients with carbapenem-resistant infections and those with carbapenem-susceptible infections. online databases were searched, and data were qualitatively synthesized and pooled in a metaanalysis. nine studies met inclusion criteria: 6 retrospective case-contro ... | 2014 | 24959688 |
| description of klebsiella quasipneumoniae sp. nov., isolated from human infections, with two subspecies, klebsiella quasipneumoniae subsp. quasipneumoniae subsp. nov. and klebsiella quasipneumoniae subsp. similipneumoniae subsp. nov., and demonstration that klebsiella singaporensis is a junior heterotypic synonym of klebsiella variicola. | strains previously classified as members of klebsiella pneumoniae phylogroups kpi, kpii-a, kpii-b and kpiii were characterized by 16s rrna (rrs) gene sequencing, multilocus sequence analysis based on rpob, fusa, gapa, gyra and leus genes, average nucleotide identity and biochemical characteristics. phylogenetic analysis demonstrated that kpi and kpiii corresponded to k. pneumoniae and klebsiella variicola, respectively, whereas kpii-a and kpii-b formed two well-demarcated sequence clusters disti ... | 2014 | 24958762 |
| allergic airway inflammation decreases lung bacterial burden following acute klebsiella pneumoniae infection in a neutrophil- and ccl8-dependent manner. | the th17 cytokines interleukin-17a (il-17a), il-17f, and il-22 are critical for the lung immune response to a variety of bacterial pathogens, including klebsiella pneumoniae. th2 cytokine expression in the airways is a characteristic feature of asthma and allergic airway inflammation. the th2 cytokines il-4 and il-13 diminish ex vivo and in vivo il-17a protein expression by th17 cells. to determine the effect of il-4 and il-13 on il-17-dependent lung immune responses to acute bacterial infection ... | 2014 | 24958709 |
| evaluation of the effectiveness of hydrogen-peroxide-based disinfectants on biofilms formed by gram-negative pathogens. | hydrogen peroxide (h2o2)-based disinfectants are widely used in a number of different healthcare settings to control bacterial colonization and contamination, and reduce the risk of cross-infection. efficacy tests of these formulations are performed on planktonic cultures, although it is well known that biofilms are the dominant form of bacterial contamination and more difficult to eradicate. | 2014 | 24957804 |
| dipeptidyl peptidase i controls survival from klebsiella pneumoniae lung infection by processing surfactant protein d. | prior work established that a deficiency in the cysteine protease dipeptidyl peptidase i (dppi) improves survival following polymicrobial septic peritonitis. to test whether dppi regulates survival from severe lung infections, dppi(-/-) mice were studied in a klebsiella pneumoniae lung infection model, finding that survival in dppi(-/-) mice is significantly better than in dppi(+/+) mice 8d after infection. dppi(-/-) mice have significantly fewer bacteria in the lung than infected dppi(+/+) mice ... | 2014 | 24955853 |
| clinical factors associated with acquisition of resistance to levofloxacin in stenotrophomonas maltophilia. | fluoroquinolones, rapidly gaining prominence in treatment of stenotrophomonas maltophilia (smp), are noted for their potency and tolerability. however, smp may rapidly acquire resistance to fluoroquinolones. we evaluated associations of clinical factors with acquisition of levofloxacin resistance (lfr) in smp. | 2014 | 24954328 |
| characteristics of kpc-2-producing klebsiella pneumoniae (st258) clinical isolates from outbreaks in 2 mexican medical centers. | the kpc-producing klebsiella pneumoniae sequence type 258 (st258) is an important pathogen widely spread in nosocomial infections. in this study, we identified the kpc-2-producing k. pneumoniae clinical isolates of 2 unrelated outbreaks that corresponded to pandemic strain st258. the isolates showed high resistance to cephalosporins, carbapenems, quinolones, and colistin. the kpc-2-producing k. pneumoniae isolates were compared to the previously studied kpc-3-producing k. pneumoniae isolates fro ... | 2014 | 24952985 |
| klebsiella pneumoniae alleviates influenza-induced acute lung injury via limiting nk cell expansion. | a protective effect induced by bacterial preinfection upon a subsequent lethal influenza virus infection has been observed, but the underlying immune mechanisms have not yet been fully elucidated. in this study, we used a mouse model of klebsiella pneumoniae preinfection to gain insight into how bacterial preinfection influences the subsequent lethal influenza virus infection. we found that k. pneumoniae preinfection significantly attenuated lung immune injury and decreased mortality during infl ... | 2014 | 24951819 |
| rapid containment of coincident outbreaks with 2 carbapenem-resistant klebsiella pneumoniae strains in an intensive care unit. | in our intensive care unit, coincident outbreaks were caused by concomitant cross-transmission of 2 carbapenem-resistant klebsiella pneumoniae strains harboring distinct mechanisms of resistance. one strain produced extended-spectrum ß-lactamase in combination with reduced permeability. the other produced oxacillinase-48 carbapenemase. rapid phenotypic detection of carbapenemase production allowed timely implementation of appropriate infection control measures. | 2014 | 24950921 |
| surveillance for infectious complications in pediatric acute liver failure - a prospective study. | to prospectively evaluate infectious complications (ic) in pediatric acute liver failure (palf) by employing surveillance cultures. | 2015 | 24944144 |
| molecular epidemiology of oxa-48-producing klebsiella pneumoniae in france. | we characterized 53 oxa-48-producing klebsiella pneumoniae (oxa-48-kp) isolated between 2011 and 2013 in 21 french hospitals. all the isolates were genotyped using mlst and pfge and the population structure of the species was determined by a nucleotide-based analysis of the entire k. pneumoniae mlst database. most of the oxa-48-kp isolates also produced ctx-m-15 and remained susceptible to imipenem and meropenem. the isolates were distributed into 20 sts, of which five were dominant (st15, st101 ... | 2014 | 24942039 |
| a carbapenem-resistant klebsiella pneumoniae outbreak following bronchoscopy. | | 2014 | 24939515 |
| tigecycline therapy for carbapenem-resistant klebsiella pneumoniae (crkp) bacteriuria leads to tigecycline resistance. | carbapenem-resistant klebsiella pneumoniae (crkp) is an increasing global threat. here, we describe the prevalence and impact of tigecycline use in a cohort of patients with crkp bacteriuria nested within a multicentre, prospective study. in the 21-month study period, 260 unique patients were included. tigecycline was given to 80 (31%) patients. the use of tigecycline during the index hospitalization was significantly associated with the subsequent development of tigecycline resistance in the sa ... | 2014 | 24931918 |
| spectroscopic investigations of the binding mechanisms between antimicrobial peptides and membrane models of pseudomonas aeruginosa and klebsiella pneumoniae. | cd spectroscopy was used to investigate the interactions of a series of synthetic amps with lps isolated from pseudomonas aeruginosa and klebsiella pneumoniae, as well as with various phospholipids to better approximate the chemical composition of the membranes of these two strains of gram-negative bacteria. this investigation was conducted in order to probe how the contributions of key physicochemical properties of an amp vary in different regions of the membranes of these two bacteria. the con ... | 2014 | 24931276 |
| klebsiella pneumoniae pharyngitis mimicking malignancy: a diagnostic dilemma. | acute pharyngitis is a common disease. however, acute pharyngitis caused by klebsiella pneumoniae with a gross appearance mimicking hypopharyngeal malignancy has never previously been reported. we report the case of a 57-year-old man with a right hypopharyngeal tumor which was disclosed by fiberoptic laryngoscopy and computed tomography scan. however, both the frozen and final pathologies showed no evidence of malignant cells, and a bacterial culture revealed the growth of k. pneumoniae. the hyp ... | 2014 | 24925476 |
| studies on the factors modulating indole-3-acetic acid production in endophytic bacterial isolates from piper nigrum and molecular analysis of ipdc gene. | the study mainly aimed quantitative analysis of iaa produced by endophytic bacteria under various conditions including the presence of extract from piper nigrum. analysis of genetic basis of iaa production was also conducted by studying the presence and diversity of the ipdc gene among the selected isolates. | 2014 | 24916921 |
| phenotypic, antimicrobial susceptibility profile and virulence factors of klebsiella pneumoniae isolated from buffalo and cow mastitic milk. | studies on the prevalence and virulence genes of klebsiella mastitis pathogens in a buffalo population are undocumented. also, the association of rmpa kfu, uge, maga, aerobactin, k1 and k2 virulent factors with k. pneumoniae buffalo, and cow mastitis is unreported. the virulence of k. pneumoniae was evaluated through both phenotypic and molecular assays. in vivo virulence was assessed by the vero cell cytotoxicity, suckling mouse assay and mice lethality test. antimicrobial susceptibility was te ... | 2014 | 24915048 |
| current concepts in combination antibiotic therapy for critically ill patients. | widespread emergence of multidrug resistant (mdr) bacterial pathogens is a problem of global dimension. mdr infections are difficult to treat and frequently associated with high mortality. more than one antibiotic is commonly used to treat such infections, but scientific evidence does not favor use of combination therapy in most cases. however, there are certain subgroups where combination therapy may be beneficial, e.g. sepsis due to carbapenem-resistant enterobacteriaceae (cre), bacteremic pne ... | 2014 | 24914260 |
| resistance to colistin associated with a single amino acid change in protein pmrb among klebsiella pneumoniae isolates of worldwide origin. | a series of colistin-resistant klebsiella pneumoniae isolates recovered from different countries was investigated in order to evaluate the involvement of the pmra/pmrb two-component system in this resistance. six isolates possessed a mutated pmrb protein, which is encoded by the pmrb gene, part of the pmrcab operon involved in lipopolysaccharide modification. the same amino acid substitution (thr157pro) in pmrb was identified in the six isolates. the six isolates belonged to four distinct clonal ... | 2014 | 24914122 |
| novel ndm-9 metallo-β-lactamase identified from a st107 klebsiella pneumoniae strain isolated in china. | | 2014 | 24913967 |
| population structure of kpc-producing klebsiella pneumoniae isolates from midwestern u.s. hospitals. | genome sequencing of carbapenem-resistant klebsiella pneumoniae isolates from regional u.s. hospitals was used to characterize strain diversity and the bla(kpc) genetic context. a phylogeny based on core single-nucleotide variants (snvs) supports a division of sequence type 258 (st258) into two distinct groups. the primary differences between the groups are in the capsular polysaccharide locus (cps) and their plasmid contents. a strict association between clade and kpc variant was found. the bla ... | 2014 | 24913165 |
| biosynthesis of 2-phenylethanol from glucose with genetically engineered kluyveromyces marxianus. | 2-phenylethanol (2-pe) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-pe is produced in a few yeast strains by ehrlich pathway. in this study, kluyveromyces marxianus was genetically engineered for overproduction of 2-pe from glucose. about 1.0g/l of 2-pe was produced by overexpressing phenylpyruvate decarboxylase (aro10) and alcohol dehydrogenase (adh2) genes of saccharomyces cerevisiae. a similar level of 2-pe was also produced from e ... | 2014 | 24910335 |
| efficacy of tigecycline, polymyxin, gentamicin, meropenem and associations in experimental klebsiella pneumoniae carbapenemase-producing klebsiella pneumoniae non-lethal sepsis. | | 2014 | 24907469 |
| mechanistic study of manganese-substituted glycerol dehydrogenase using a kinetic and thermodynamic analysis. | mechanistic insights regarding the activity enhancement of dehydrogenase by metal ion substitution were investigated by a simple method using a kinetic and thermodynamic analysis. by profiling the binding energy of both the substrate and product, the metal ion's role in catalysis enhancement was revealed. glycerol dehydrogenase (gdh) from klebsiella pneumoniae sp., which demonstrated an improvement in activity by the substitution of a zinc ion with a manganese ion, was used as a model for the me ... | 2014 | 24896258 |
| upregulation of transcripts for metabolism in diverse environments is a shared response associated with survival and adaptation of klebsiella pneumoniae in response to temperature extremes. | klebsiella pneumoniae being ubiquitous in nature encounters wide differences in environmental condition. the organism's abundance in natural water reservoirs exposed to temperature variation forms the basis of its persistence and spread in the soil and other farm produce. in order to investigate the effect of temperature changes on the survival and adaptation of the bacteria, the transcriptional response of k. pneumoniae subjected to low (20 °c) and high (50 °c) temperature shock were executed u ... | 2014 | 24890397 |
| treating infections caused by carbapenemase-producing enterobacteriaceae. | carbapenemase-producing enterobacteriaceae (cpe) have spread worldwide, causing serious infections with increasing frequency. cpe are resistant to almost all available antibiotics, complicating therapy and limiting treatment options. mortality rates associated with cpe infections are unacceptably high, indicating that the current therapeutic approaches are inadequate and must be revised. here, we review 20 clinical studies (including those describing the largest cohorts of cpe-infected patients) ... | 2014 | 24890393 |
| chemical composition, antimicrobial and antibiofilm activity of the essential oil and methanol extract of the mediterranean cypress (cupressus sempervirens l.). | cupressus sempervirens is a medicinal plant traditional, its dried leaves are used in treatment of stomach pain, diabetes, inflammation, toothache, laryngitis and as contraceptive. | 2014 | 24890383 |
| bloodstream infections and sepsis in greece: over-time change of epidemiology and impact of de-escalation on final outcome. | choice of empirically prescribed antimicrobials for sepsis management depends on epidemiological factors. the epidemiology of sepsis in greece was studied in two large-periods. | 2014 | 24885072 |
| molecular characterization of clinical multidrug-resistant klebsiella pneumoniae isolates. | klebsiella pneumoniae is a frequent nosocomial pathogen, with the multidrug-resistant (mdr) k. pneumoniae being a major public health concern, frequently causing difficult-to-treat infections worldwide. the aim of this study was to investigate the molecular characterization of clinical mdr klebsiella pneumoniae isolates. | 2014 | 24884610 |
| [optimized cultivation of a bioflocculant m-c11 produced by klebsiella pneumoniae and its application in sludge dewatering]. | a bioflocculant-producing klebsiella pneumoniae strain c11 was screened out from activated sludge and the optimal medium conditions for the production of microbial flocculant m-c11 were determined. the bioflocculant was used in activated sludge dewatering and compared with conventional chemical conditioners. effects of ph, cacl2 dosages and m-c11 dosages on sludge dewaterability were investigated. the optimized conditions for m-c11 production indicated that the optimal medium carbon, nitrogen, m ... | 2014 | 24881415 |
| favourable outcome in the treatment of carbapenem-resistant enterobacteriaceae urinary tract infection with high-dose tigecycline. | | 2014 | 24879666 |
| surveillance trends of carbapenem-resistant enterobacteriaceae from singapore, 2010-2013. | trends in carbapenem-resistant enterobacteriaceae (cre) collected from hospitals nationwide in singapore over 3 years are presented. hospital isolates with imipenem or meropenem minimum inhibitory concentrations (mics) of >1mg/l were sent to the national public health laboratory for further investigation. a total of 400 cre were submitted, 227 (56.8%) of which carried a carbapenemase gene. blandm was the most common (130/400; 32.5%), followed by blaoxa-48-like (blaoxa-48, -181, -232) (55/400; 13 ... | 2014 | 27873598 |
| exogenous pulmonary surfactant as a vehicle for antimicrobials: assessment of surfactant-antibacterial interactions in vitro. | owing to its unique surface-active properties, an exogenous pulmonary surfactant may become a promising drug delivery agent, in particular, acting as a vehicle for antibiotics in topical treatment of pneumonia. the purpose of this study was to assess a mutual influence of natural surfactant preparation and three antibiotics (amikacin, cefepime, and colistimethate sodium) in vitro and to identify appropriate combination(s) for subsequent in vivo investigations of experimental surfactant/antibioti ... | 2014 | 24876994 |
| characterization of carbapenem resistance mechanisms in klebsiella pneumoniae and in vitro synergy of the colistin-meropenem combination. | in this prospective study, consecutive isolates of klebsiella pneumoniae were tested for different mechanisms of carbapenem resistance using the modified hodge test (mht), rosco neo-sensitabs (rosco). phenylalanine arginine beta-naphthylamide assay (pabn) inhibitor-based test was done on isolates in which the mechanism of resistance was not identifiable by the rosco. among 105 selected isolates, carbapenemase production was noted in 100 (95%) by mht and rosco showed 97 (92·4%) inhibition with di ... | 2015 | 24871673 |
| carbapenem-resistant klebsiella pneumoniae strains exhibit diversity in aminoglycoside-modifying enzymes, which exert differing effects on plazomicin and other agents. | we measured in vitro activity of plazomicin, a next-generation aminoglycoside, and other aminoglycosides against 50 carbapenem-resistant klebsiella pneumoniae strains from two centers and correlated the results with the presence of various aminoglycoside-modifying enzymes (ames). ninety-four percent of strains were sequence type 258 (st258) clones, which exhibited 5 ompk36 genotypes; 80% and 10% of strains produced klebsiella pneumoniae carbapenemase 2 (kpc-2) and kpc-3, respectively. ninety-eig ... | 2014 | 24867988 |
| nosocomial meningitis caused by klebsiella pneumoniae producing carbapenemase, with initial cerebrospinal fluid minimal inflammatory response. | | 2014 | 24863522 |
| investigation of class 1 integrons in klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in recife, state of pernambuco. | the high prevalence of klebsiella pneumoniae infections is related to the ability of k. pneumoniae to acquire and disseminate exogenous genes associated with mobile elements, such as r plasmids, transposons and integrons. this study investigated the presence of class 1 integrons in clinical and microbiota isolates of k. pneumoniae belonging to different phylogenetic groups and correlated these results with the antimicrobial resistance profiles of the studied isolates. | 2014 | 24861289 |
| emergence of carbapenem-resistant klebsiella pneumoniae strains producing kpc-3 in brescia hospital, italy. | carbapenem-resistant k. pneumoniae has recently been reported as a new multidrug-resistant nosocomial pathogen. this study reports the emergence of carbapenem-resistant k. pneumoniae strains in brescia civic hospital, italy. different samples, collected from april 2012 to february 2013, showed that 29 patients presented infections from multidrug-resistant k. pneumoniae and three of these patients were intestinal carriers. in total, 40 carbapenem-resistant k. pneumoniae strains were isolated from ... | 2014 | 24858644 |
| molecular mechanisms of β-lactam resistance in carbapenemase-producing klebsiella pneumoniae from sri lanka. | carbapenemases are increasingly important antimicrobial resistance determinants. little is known about the carbapenem resistance mechanisms in sri lanka. we examined 22 carbapenem-resistant klebsiella pneumoniae from sri lanka to determine their β-lactam resistance mechanisms. the predominant resistance mechanisms we detected in this study were oxa-181, ndm-1 carbapenemases and extended-spectrum β-lactamase ctx-m-15. all isolates were then genotyped by pulsed-field gel electrophoresis, variable- ... | 2014 | 24855071 |
| native valve endocarditis due to extended spectrum β-lactamase producing klebsiella pneumoniae. | | 2014 | 24851078 |
| multi-drug-resistant klebsiella pneumoniae pancreatitis: a new challenge in a serious surgical infection. | klebsiella pneumoniae is an important cause of nosocomial infections, but its role in severe acute pancreatitis (sap) is not well defined. few cases of k. pneumoniae associated sap have been reported. due to the emergence of extended-spectrum beta-lactamases (esbls) and carbapenemases, treatment of multidrug-resistant (mdr) k. pneumoniae presents a challenge. tigecycline and colistin have gained recent attention for their broad-spectrum antimicrobial activity. | 2015 | 24850293 |
| clinical and bacteriological influence of diabetes mellitus on deep neck infection: systematic review and meta-analysis. | diabetes mellitus has been recognized as the most common systemic disease associated with deep neck infection. we report the first systematic review and meta-analysis of the influence of diabetes on clinical and bacteriological characteristics of deep neck infection. | 2015 | 24844194 |
| transferable fluoroquinolone resistance in enterobacteriaceae and pseudomonas aeruginosa isolated from hemocultures. | the main mechanisms causing high-level resistance to fluoroquinolones (fq) are encoded chromosomally; that includes mutations in genes coding dna-gyrase, but overexpression of efflux pumps contributes to increased minimum inhibitory concentration (mic) of fq as well. however, genes responsible for fq-resistance may be harboured in transferable/conjugative plasmids. for some time, there was an assumption that resistance to fq cannot be transferable in conjugation due to their synthetic origin, un ... | 2014 | 24844110 |
| klebsiella pneumoniae sequence type 11 isolate producing rmtg 16s rrna methyltransferase from a patient in miami, florida. | | 2014 | 24841274 |
| in vivo evolution to colistin resistance by pmrb sensor kinase mutation in kpc-producing klebsiella pneumoniae is associated with low-dosage colistin treatment. | colistin is a key drug for the treatment of infections caused by extensively drug-resistant strains of enterobacteriaceae producing carbapenemases. however, the emergence of colistin resistance is being increasingly reported, especially among klebsiella pneumoniae strains producing kpc-type carbapenemases (kpc-kp). in this work, we investigated colistin-susceptible (kpb-1) and colistin-resistant (kpb-2) sequential isolates obtained from a patient with a kpc-kp infection before and after low-dosa ... | 2014 | 24841267 |
| enhancement of 1,3-propanediol production by expression of pyruvate decarboxylase and aldehyde dehydrogenase from zymomonas mobilis in the acetolactate-synthase-deficient mutant of klebsiella pneumoniae. | the acetolactate synthase (als)-deficient mutant of klebsiella pneumoniae fails to produce 1,3-propanediol (1,3-pd) or 2,3-butanediol (2,3-bd), and is defective in glycerol metabolism. in an effort to recover production of the industrially valuable 1,3-pd, we introduced the zymomonas mobilis pyruvate decarboxylase (pdc) and aldehyde dehydrogenase (aldb) genes into the als-deficient mutant to activate the conversion of pyruvate to ethanol. heterologous expression of pdc and aldb efficiently recov ... | 2014 | 24841211 |
| rectus femoris pyomyositis caused by klebsiella pneumoniae. | | 2014 | 24824052 |
| diversity of capsular polysaccharide gene clusters in kpc-producing klebsiella pneumoniae clinical isolates of sequence type 258 involved in the italian epidemic. | strains of klebsiella pneumoniae producing kpc-type beta-lactamases (kpc-kp) are broadly disseminating worldwide and constitute a major healthcare threat given their extensively drug resistant phenotypes and ability to rapidly disseminate in healthcare settings. in this work we report on the characterization of two different capsular polysaccharide (cps) gene clusters, named cpsbo-4 and cps207-2, from two kpc-kp clinical strains from italy belonging in sequence type (st) 258, which is one of the ... | 2014 | 24823690 |
| hygromycin-resistance vectors for gene expression in pichia pastoris. | pichia pastoris is a common host organism for heterologous protein expression and metabolic engineering. zeocin-, g418-, nourseothricin- and blasticidin-resistance genes are the only dominant selectable markers currently available for selecting p. pastoris transformants. we describe here new p. pastoris expression vectors that confer a hygromycin resistance base on the klebsiella pneumoniae hph gene. to demonstrate the application of the vectors for intracellular and secreted protein expression, ... | 2014 | 24822243 |
| mortality risk score for klebsiella pneumoniae bacteraemia. | klebsiella pneumoniae bacteraemia (kpb) has been associated with multiple risk factors. however association of these risk factors with mortality secondary to kpb has been poorly documented. | 2014 | 24814431 |
| whole genome mapping of the first reported case of kpc-2-positive klebsiella pneumoniae st258 in nebraska. | three ertapenem-resistant klebsiella pneumoniae carrying bla(kpc-2) were isolated from a single patient in nebraska over a span of 5 months. a comparative analysis of the genetic relatedness of these isolates was investigated using pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome mapping. | 2014 | 24813687 |
| in vitro and in vivo activities of piperacillin-tazobactam and meropenem at different inoculum sizes of esbl-producing klebsiella pneumoniae. | the inoculum effect is a laboratory phenomenon in which the minimal inhibitory concentration (mic) of an antibiotic is increased when a large number of organisms are exposed. due to the emergence of extended-spectrum β-lactamase-producing klebsiella pneumoniae (esbl-kpn) infections, the inoculum effect of esbl-kpn on β-lactams was studied in vitro and in vivo using an experimental model of pneumonia. the in vitro inoculum effect of 45 clinical esbl-kpn isolates on β-lactams was evaluated at stan ... | 2014 | 24813594 |
| microbiological profile of lower respiratory tract infections in neurological intensive care unit of a tertiary care center from central india. | lower respiratory tract infections (lrti's) are the most frequent infections among patients in intensive care units. the consequences of increased drug resistance are far reaching since bacterial infection of the lower respiratory tract (lrt) is a major cause of death from infectious disease. | 2013 | 24808671 |
| expansion and evolution of a virulent, extensively drug-resistant (polymyxin b-resistant), qnrs1-, ctx-m-2-, and kpc-2-producing klebsiella pneumoniae st11 international high-risk clone. | in this study, we report the early expansion, evolution, and characterization of a multiresistant klebsiella pneumoniae clone that was isolated with increasing frequency from inpatients in a tertiary-care university hospital in brazil. seven carbapenem- and quinolone-resistant and polymyxin b-susceptible or -resistant k. pneumoniae isolates isolated between december 2012 and february 2013 were investigated. beta-lactamase- and plasmid-mediated quinolone resistance (pmqr)-encoding genes and the g ... | 2014 | 24808234 |
| clonal spread of klebsiella pneumoniae producing kpc-2 beta-lactamase in croatian university hospital. | | 2015 | 24806872 |
| molecular epidemiology and virulence factors of pyogenic liver abscess causing klebsiella pneumoniae in china. | the molecular epidemiology and prevalence of virulence factors of isolates from patients with klebsiella pneumoniae liver abscess (kla) in mainland china are unknown. klebsiella pneumoniae isolates were obtained from drainage samples aseptically collected from patients with pyogenic liver abscess (pla). the genetic similarity of kla isolates was analyzed by pulsed-field gel electrophoresis. the hypermucoviscosity (hv) phenotype was identified by a positive string test. the k1 and k2 genotypes, t ... | 2014 | 24804560 |
| surveillance of carbapenem-resistant klebsiella pneumoniae: tracking molecular epidemiology and outcomes through a regional network. | carbapenem resistance in gram-negative bacteria is on the rise in the united states. a regional network was established to study microbiological and genetic determinants of clinical outcomes in hospitalized patients with carbapenem-resistant (cr) klebsiella pneumoniae in a prospective, multicenter, observational study. to this end, predefined clinical characteristics and outcomes were recorded and k. pneumoniae isolates were analyzed for strain typing and resistance mechanism determination. in a ... | 2014 | 24798270 |
| toll-like receptor 6 v327m polymorphism is associated with an increased risk of klebsiella pneumoniae infection. | klebsiella pneumoniae is a common cause of nosocomial pneumonia, especially in children. toll-like receptors plays an important role in defense against this pathogen. the impact of human tlr6 polymorphisms on susceptibility to k. pneumoniae infection is poorly understood. the aim of the present work was to determine whether single nucleotide polymorphisms in tlr6 are associated with altered immune responses to k. pneumoniae. | 2014 | 24797996 |
| direct application of maldi-tof mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis. | bacterial meningitis is a neurological emergency. early diagnosis and rapid initiation of antimicrobial therapy are vital. | 2014 | 24797349 |
| brief communication: detection of clinical klebsiella pneumoniae isolates from china containing transferable quinolone resistance determinants exhibiting resistance to both aminoglycoside and β-lactams. | though aminoglycosides are routinely used clinically as antimicrobial agents for the treatment of severe infections due to klebsiella pneumoniae, resistance to the same is an increasing problem. one such resistance mechanism is the production of 16s rrna methylases. the objective of the current study was to investigate the prevalence and molecular epidemology of 16s rrna methylase genes among 43 k. pneumoniae isolates (each of which had at least one pqmr gene and ciprofloxacin minimum inhibitory ... | 2014 | 24795066 |
| colistin resistance in klebsiella pneumoniae. | increasing use of colistin for multidrug-resistant gram-negative bacterial infections has led to the emergence of colistin resistance in klebsiella pneumoniae in several countries worldwide, including europe (especially greece), and colistin resistance rates are continually increasing. heteroresistance rates, which were significantly higher than resistance rates, were found to be important. although the mechanism underlying resistance is unclear, it has been suggested that it is related to lipop ... | 2014 | 24794735 |
| lung abscess due to streptococcus pneumoniae: a case series and brief review of the literature. | anaerobes used to be the most common cause of community-acquired lung abscess, and streptococcus species used to be the second most common cause. in recent years, this has been changing. klebsiella pneumoniae is now an increasing cause of community- acquired lung abscess, but streptococcus species continue to be major pathogens. necrotizing pneumonia has generally been regarded as a rare complication of pneumococcal infection in adults. type 3 streptococcus pneumoniae was the single most common ... | 2014 | 24793153 |
| outbreak of carbapenemase-producing klebsiella pneumoniae neurosurgical site infections associated with a contaminated shaving razor used for preoperative scalp shaving. | between july 5 and 21, 2011, an outbreak of neurosurgical site infections with carbapenemase-producing klebsiella pneumonia occurred in a tertiary care hospital. the outbreak affected 7 patients. the subsequent investigation revealed that a barber's contaminated shaving razor may have caused the carbapenemase-producing klebsiella pneumonia outbreak. standardized skin preparation performed by registered nurses using sterilized instruments should be emphasized. | 2014 | 24792718 |
| performance of quantification of modified hodge test: an evaluation with klebsiella pneumoniae carbapenemase-producing enterobacteriaceae isolates. | modified hodge test (mht) has been suggested as screening tests for carbapenemases, but concerns regarding its difficult interpretation and common false-positive results obtained in the presence of other β -lactamases have been noted. this study aimed to quantify the enhanced growth formed by the indicator strain and thus evaluate the performance of a quantitative interpretation of mht for kpc screening. mht was performed in 50 kpc-producing isolates and 334 non-carbapenemase-producing isolates, ... | 2014 | 24790988 |
| antibacterial activities of plants from central africa used traditionally by the bakola pygmies for treating respiratory and tuberculosis-related symptoms. | the antibacterial activities of 18 plants from 10 different families were investigated for their antimicrobial efficacy, based on the traditional uses of these species by bakola pygmies living in central africa, especially along the ngoyang area in cameroon for the treatment of respiratory and tuberculosis-related symptoms. the aim of the study is to test the antimicrobial efficacy of these plants against some pathogens associated with respiratory disease and to determine if there is any validat ... | 2014 | 24786571 |
| healthcare-associated klebsiella pneumoniae carbapenemase producing k. pneumoniae bloodstream infection: the time has come. | | 2014 | 24771335 |
| endogenous klebsiella pneumoniae endophthalmitis. | klebsiella pneumonia is a common human pathogen, and endogenous endophthalmitis is a vision-threatening infection presentedwith pain, redness, decreased vision acuity, and intraocular inflammation. endogenous endophthalmitis caused by klebsiella pneumoniae is uncommon and usually happens in patients with immunosuppression conditions. diabetes is a predisposing risk factor, and liver abscess is a major source of klebsiella pneumonia endogenous endophthalmitis (kpee). here, we report a case of kpe ... | 2014 | 24768670 |
| [the threat of the carbapenemase-producing enterobacteriaceae in spain: positioning report of the seimc study groups, geih and gemara]. | the emergence and spread of carbapenemase-producing enterobacteriaceae (cpe), as the current paradigm of extensive drug-resistance and multi-drug resistance to antibiotics, is a serious threat to patient health and public health. the increase in oxa-48- and vim-1-producing klebsiella pneumoniae isolates represents the greatest impact of cpe in spain. this evidence has lead the members of a representative panel of the spanish study groups of nosocomial infections and mechanisms of action and resi ... | 2014 | 24767691 |
| endogenous klebsiella pneumoniae endophthalmitis associated with intravenous drug abuse. | | 2014 | 24762614 |
| spread of klebsiella pneumoniae carbapenemase-2-producing klebsiella pneumoniae clones in asia. | | 2014 | 24762302 |
| first description of ndm-1-, kpc-2-, vim-2- and imp-4-producing klebsiella pneumoniae strains in a single chinese teaching hospital. | a total of 180 non-duplicate carbapenem-resistant klebsiella pneumoniae isolates were recovered from patients hospitalized between december 2010 and january 2012 at a chinese hospital. eight kpc-2, four ndm-1, one vim-2, and five kpc-2 plus imp-4 producers were identified and all were multidrug resistant due to the presence of other resistance determinants, including extended-spectrum β-lactamases (ctx-m-15, shv-12), 16s rrna methylases (arma, rmtb) and plasmid-mediated quinolone-resistance dete ... | 2015 | 24762211 |
| cross-infection of solid organ transplant recipients by a multidrug-resistant klebsiella pneumoniae isolate producing the oxa-48 carbapenemase, likely derived from a multiorgan donor. | we describe two cases of bacteremic infections caused by a multidrug-resistant klebsiella pneumoniae isolate producing the oxa-48 carbapenemase that occurred in two solid organ transplant (liver and kidney) recipients, which was apparently transmitted with the allografts. this finding underscores the risk of donor-derived infections by multidrug-resistant gram-negative pathogens in solid organ transplant recipients and emphasizes the need for rapid screening of organ donors for carriage of simil ... | 2014 | 24759725 |
| the role of nanotechnology in combating multi-drug resistant bacteria. | the development of antibiotics has played a significant role in combating the dreaded infectious disease such as tuberculosis, pneumonia, typhoid fever and meningitis in 20th century. however, the improper use of antibiotics led to the development of multidrug resistance (mdr) in microbial flora raising a global public health concern of 21st century. this unforeseen threat demands the development of new drugs and strategies for combating antibiotic resistance shown by many microbial species. rec ... | 2014 | 24757944 |
| clinical and microbiological characteristics of klebsiella pneumoniae from community-acquired recurrent urinary tract infections. | understanding the pathogenesis of recurrent urinary tract infection (ruti) and whether it is attributable to reinfection with a new strain or relapse with the primary infecting strain is of considerable importance. because previous studies regarding community-acquired klebsiella pneumoniae ruti are inconclusive, we undertook this study to evaluate the characteristics of the host and the bacterial agent k. pneumoniae in ruti. a prospective study was designed, using consecutive patients diagnosed ... | 2014 | 24756209 |
| inch-type plasmid harboring bla ctx-m-15, bla dha-1, and qnrb4 genes recovered from animal isolates. | the whole sequence of plasmid penva carrying the extended-spectrum β-lactamase gene blactx-m-15 was determined. it was identified from a series of clonally related klebsiella pneumoniae sequence type 274 strains recovered from companion animals. this plasmid was 253,984 bp in size and harbored, in addition to blactx-m-15, a large array of genes encoding resistance to many antibiotic molecules, including β-lactams (blatem-1, bladha-1), aminoglycosides (aaca2, aada1), tetracycline (teta), quinolon ... | 2014 | 24752252 |
| molecular characterization of klebsiella pneumoniae isolated from renal transplanted patients: virulence markers, extended-spectrum β-lactamases, and genetic relatedness. | the objective was to characterize virulence markers and β-lactam resistance in klebsiella pneumoniae isolates from renal transplant patients and to evaluate their genetic relatedness. two main genetic lineages were detected: 1 carried bla(ctx-m-15) not associated to incfiia plasmid replicon, which was found on the other lineage not expressing ctx-m-type enzyme. pai iii(536) and pai ii(cft073) were detected for the first time in k. pneumoniae in 1 clone, while the siderophore kfu was carried by t ... | 2014 | 24746826 |
| investigation of the first outbreak of oxa-48-producing klebsiella pneumoniae in ireland. | carbapenemase-producing enterobacteriaceae (cpe) strains are encountered with increasing frequency in europe. in november 2010 the european centre for disease control (ecdc) graded ireland as only having sporadic occurrence of cpe. | 2014 | 24746608 |
| presence of quinolone resistance to qnrb1 genes and blaoxa-48 carbapenemase in clinical isolates of klebsiella pneumoniae in spain. | a study is presented on the presence of quinolone resistance qnrb1 genes in clinical isolates belonging to the largest series of infections caused by oxa-48-producing klebsiella pneumoniae in a single-centre outbreak in spain. evidence is also provided, according to in vitro results, that there is a possibility of co-transfer of plasmid harbouring blaoxa-48 with an other plasmid harbouring qnrb1 in presence of low antibiotic concentrations of fluoroquinolones, showing the risk of multi-resistanc ... | 2014 | 24746402 |
| successful control of a neonatal outbreak caused mainly by st20 multidrug-resistant shv-5-producing klebsiella pneumoniae, greece. | extended spectrum beta-lactamase-producing klebsiella pneumoniae (esbl-kp) infection can cause significant morbidity and mortality in neonates. we investigated a nosocomial esbl-kp outbreak in a neonatal intensive care unit (nicu) of the university hospital of larissa (uhl), central greece. | 2014 | 24742105 |
| fatal klebsiella pneumoniae meningitis in a patient with diabetes mellitus and hansen's disease. | klebsiella is a gram-negative bacterium that causes different types of health care-associated infections including pneumonia, bloodstream infections, surgical site infections and meningitis. we report here a case of klebsiella pneumoniae meningitis in a patient with diabetes mellitus and hansen's disease. a middle-aged man with a known case of diabetes mellitus and hansen's disease presented with the complaints of blurred vision in the left eye and the patient was found to have cataract. patient ... | 2014 | 24739855 |
| multidrug resistant ndm-1 metallo-beta-lactamase producing klebsiella pneumoniae sepsis outbreak in a neonatal intensive care unit in a tertiary care center at central india. | the objective of the following study is to detect genes encoding carbapenem resistance in klebsiella pneumoniae sepsis outbreak in a neonatal intensive care unit (nicu). | 2014 | 24739834 |
| the balkan region: ndm-1-producing klebsiella pneumoniae st11 clonal strain causing outbreaks in greece. | despite the fact that the ndm-1 carbapenemase has successfully disseminated worldwide, outbreaks remain uncommon in the european region. we describe the characteristics of the first outbreaks caused by ndm-1-producing klebsiella pneumoniae clonal isolates in greece. | 2014 | 24739146 |
| predictive models for identification of hospitalized patients harboring kpc-producing klebsiella pneumoniae. | the production of klebsiella pneumoniae carbapenemases (kpcs) by enterobacteriaceae has become a significant problem in recent years. to identify factors that could predict isolation of kpc-producing k. pneumoniae (kpckp) in clinical samples from hospitalized patients, we conducted a retrospective, matched (1:2) case-control study in five large italian hospitals. the case cohort consisted of adult inpatients whose hospital stay included at least one documented isolation of a kpckp strain from a ... | 2014 | 24733460 |
| diabetes with pyogenic liver abscess--a perspective on tongue assessment in traditional chinese medicine. | this novel study provides a time series analysis of tongue features extracted from a diabetic patient with pyogenic liver abscess (pla), treated with the integration of western medicine and traditional chinese medicine (tcm). the features, namely, tongue color, tongue fur thickness and fur color, identified from a series of tongue images taken every two days, exhibit significant transitions matching closely with the progression of disease. these tongue features could serve as effective, non-intr ... | 2014 | 24731906 |
| the detection and verification of carbapenemases using ertapenem and matrix assisted laser desorption ionization-time of flight. | the increase in carbapenemase producing enterobacteriaceae and pseudomonas aeruginosa is a significant threat to modern medicine. a rapid detection of carbapenemase production in klebsiella pneumoniae and pseudomonas aeruginosa is of importance for the institution of correct antibiotic treatment and infection control measures. | 2014 | 24720586 |
| characterization of the blakpc-2 and blakpc-3 genes and the novel blakpc-15 gene in klebsiella pneumoniae. | three klebsiella pneumoniae isolates exhibiting high-level resistance to carbapenem were analysed by pcr, pfge, gene mapping, plasmid conjugation and southern blot hybridization using a blakpc probe. in addition to the frequently reported blakpc-2 and blakpc-3 genes, a novel blakpc-15 gene was identified in one of the isolates. the results of plasmid analysis and southern blot hybridization revealed that the three blakpc genes were located on transferable plasmids exhibiting three different patt ... | 2014 | 24713357 |
| lung adenocarcinoma masquerading as refractory klebsiella pneumoniae. | we report the case of a middle-aged man where a diagnosis of klebsiella pneumoniae obscured the underlying malignancy. the patient was hospitalised for management of a presumed refractory community-acquired pneumonia with radiological features of right lower lobe consolidation. bronchoscopy did not identify an endobronchial lesion and washings grew k pneumoniae. ct-guided fine-needle aspirate samples did not detect any malignancy. however, despite appropriate antibiotic treatment there was no im ... | 2014 | 24706703 |
| risk factors for carbapenem-resistant klebsiella pneumoniae infection/colonization: a case-case-control study. | carbapenem-resistant klebsiella pneumoniae (crkp) is increasingly reported worldwide. the aim of the present study was to identify risk factors associated with the development of crkp infections. a retrospective, case-case-control study was performed at the university hospital of heraklion, greece. the study population included 83 patients from whom crkp was isolated, 79 from whom carbapenem-sensitive k. pneumoniae (cskp) was isolated and 161 (control group) from whom k. pneumoniae was not isola ... | 2014 | 24703709 |
| fe(iii) oxides accelerate microbial nitrate reduction and electricity generation by klebsiella pneumoniae l17. | klebsiella pneumoniae l17 is a fermentative bacterium that can reduce iron oxide and generate electricity under anoxic conditions, as previously reported. this study reveals that k. pneumoniae l17 is also capable of dissimilatory nitrate reduction, producing no2(-), nh4(+), no and n2o under anoxic conditions. the presence of fe(iii) oxides (i.e., α-feooh, γ-feooh, α-fe2o3 and γ-fe2o3) significantly accelerates the reduction of nitrate and generation of electricity by k. pneumoniae l17, which is ... | 2014 | 24703664 |
| broth microdilution methods using b-lactamase inhibitors for the identification of klebsiella pneumoniae carbapenemases and metallo-β-lactamases in gram-negative bacilli. | this study was designed to evaluate the performance of the broth microdilution (bmd) test in detecting the production of klebsiella pneumoniae carbapenemases (kpcs) and metallo-β-lactamases (mbls) in clinical isolates of gram-negative bacilli by using various β-lactamase inhibitors. a carbapenemase detection test, comprised of mueller-hinton broth containing serial twofold dilutions of imipenem or meropenem with and without aminophenylboronic acid (apb), phenylboronic acid (pb), cloxacillin (clx ... | 2014 | 24695474 |
| first case of liver abscess in scandinavia due to the international hypervirulent klebsiella pneumoniae clone st23. | this is the first case report from scandinavia of a pyogenic liver abscess caused by a klebsiella pneumoniae isolate belonging to the international hyper virulent clone st23. the patient, an 85-year old caucasian, had no history of foreign travel or any classical predisposing factors for infection. the isolate was hypermucoviscous of capsular serotype k1 and carried the virulence factors aerobactin, alls, kfu and rmpa. | 2014 | 24688609 |