Publications

surface ruffles as markers for studies of cell transformation by rous sarcoma virus.	confluent chick embryo fibroblasts infected with the ts68 mutant of rous sarcoma virus were examined by scanning electron microscopy at the permissive (36 degrees) and nonpermissive (41 degrees) temperatures for transformation. infected cells shifted from 41 degrees to 36 degrees undergo a change in shape from elongated to rounded. this process is preceded by the appearance of surface ruffles on the cell. these surface ruffles are not observed on cells maintained at 41 degrees, appear as early a ...	1975	171654
[effect of the method of cell disintegration on the aspartate kinase activity of preparations from bacillus polymyxa var. ross].	the influence of methods of cell disintegration of bac. polymyxa on aspartate kinase activity (ec 2.7.2.4) was examined. the disruption by means of the hews press yielded a more active preparation as compared with ultrasonic disintegration. the supernatant treatment with streptomycin sulphate increased the preparation activity 2-fold. dialysis of the fraction with a high activity of aspartate kinase inactivated the enzyme by 80--85%. the relationship between the aspartate kinase activity and the ...	1975	171645
predicted distribution of nad domain among glycolytic enzymes.		1975	171581
cutaneous benign histiocytoses of childhood.		1990	171560
information contained in the amino acid sequence of the alpha1(i)-chain of collagen and its consequences upon the formation of the triple helix, of fibrils and crosslinks.	the molecule of type i collagen from skin consists of two alpha1(i)-chains and one alpha2-chain. the sequence of the entire alpha1-chain comprising 1052 residues is summarily presented and discussed. apart from the 279 residues of alpha1(i)-cb8 whose sequence has been established for rat skin collagen, all sequences have been determined for calf skin collagen. in order to facilitate sequence analysis, the alpha1-chain was cleaved into defined fragments by cyanogen bromide or hydroxylamine or lim ...	1975	171554
the separation of cells and subcellular particles by colloidal silica density gradient centrifugation.		1990	171541
"unbound" ligand adsorption on dextran-coated charcoal: practical considerations.		1975	171517
circular dichroism estimate of secondary structure of glutamate dehydrogenase.		1989	171417
sebaceous cell carcinoma of the parotid gland.	sebaceous cell carcinoma of the parotid gland is a rare lesion. a review of the english literature reveals nine previously reported cases. two cases are presented in which the tumor was originally diagnosed as mucoepidermoid carcinoma. aggressive treatment of the recurrence, which was subsequently recognized as sebaceous cell carcinoma has resulted in apparent cure. histologic features and possible histogenesis are discussed.	1977	170840
splenocyte plaque assay for the detection of murine leukemia virus.	a modified xc assay for murine leukemia virus (mulv) employing splenocytes taken directly from the animal is described. this modification can be more than 1000 times more sensitive than xc plaque assays employing tissue extracts. this technique should lend itself readily to the quantitation of infectious mulv in defined populations of lymphoid cells.	1975	170622
some properties of androgen-binding activity in rat testis.	high-affinity (ka approximately equal to 5 x 10(8) m-1 for testosterone) androgen-binding activity in rat testis was shown to have a rapid dissociation rate constant (t1/2 = 3 min, 0 degrees c, 30% glycerol buffer) using dextran-coated charcoal to separate bound from free hormone. because of this fact, exchange of endogenous and labeled hormone was complete in the assay incubation time (16 h, 0 degrees c) and scatchard plots of the high-affinity binding data were shown to measure total as contra ...	1975	170151
dispersion along fiber tracts and in the coupling between tracts and a cortical network.	dispersion in a neuronal coritcal network was modeled using csmp, a continuous systems modeling program. the signal dispersion over pathways was simulated by use of a serial product approximating the convolution integral. the program was written in a sufficiently general format to be applied to a variety of biological signals. calculated signals from a fiber tract and activation of a cortical network were compared with experimental data from cats. the network consists of excitatory cells in a fo ...	1975	170035
ultrastructure of human bronchiolo-alveolar cell carcinoma.	ultrastructural features were correlated with a series of special staining reactions in eight cases of bronchiolo-alveolar carcinoma. ultrastructural, all tumors were similarly composed of large cells with abundant cytoplasm and small nuclei in close contact with each other. straight membranes or complex interdigitations occurred within adjacent tumor cells, attached to each other by scattered desmosomes. microvilli or cilia abutted from free surfaces of the cells, and were noted in different st ...	1975	169987
a spin-label study on fusion of red blood cells induced by hemagglutinating virus of japan.	fusion of red blood cells (rbc) induced by hemagglutinating virus of japan (hvj) has been studied using a phosphatidylcholine spin label. the spin label was readily incorporated and diffused into the lipid bilayer portion of the viral envelope. the exchange broadening in the electron spin resonance (esr) spectrum of densely labeled virus disappeared rapidly when the virus was mixed with rbc at 37 degrees. the spectrum gradually approached that of the host cell spin labeled with the phosphatidylc ...	1975	169884
metabolism of d- and l-norgestrel in humans.	levels of dl-norgestrel, d-norgestrel, l-norgestrel and norethisterone were measured in blood by radioimmunoassay after oral administration of the progestin to 3 healthy male subjects and the half-lives calculated. half-lives for d-norgestrel were much lower than those of l-norgestrel and similar to those of norethisterone. measurement of norgestrel in blood can therefore be misleading unless the biologically active d-isomer is estimated. since there was no difference in half-lives between norge ...	1976	169856
the effect of ascorbic acid on lysyl and prolyl hydroxylase activity of cultured fibroblasts.		1976	169749
effect of klebsiella pneumoniae enterotoxin on intestinal transport in the rat.	the effects on intestinal transport of either a semipurified preparation of enterotoxin elaborated by klebsiella pneumoniae or similaryly prepared control material were tested by marker perfusion studies in the small intestine of rats. at a concentration of 2 mg/ml, the enterotoxin produced net secretion of water, na, and cl in both jejunal and ileal segments; hco3 transport was not affected. net secretion was evident within 30 min after intorduction of the toxin and was maximal after 90 min. th ...	1975	169297
kinetic parameters of tryptophan: trna ligase catalyzed atp-(32p) pyrophosphate exchange as an approach to extimation of the order of substrate binding.		1975	169163
integration of avian sarcoma virus specific dna in mammalian chromatin.		2003	169136
structural studies on two adenovirus 2-sv40 hybrids containing the entire sv40 genome.		1975	169085
a simplifying concept in tumor virology: virus-specific "pleiotropic effectors".		1975	169084
sv40 t antigen: partial purification and properties.		2003	169071
the role of cell surface changes in rna tumor virus-transformed cells.		1975	169020
nonchromaffin paraganglioma (chemodectoma) of thyroid region.	a case of a paraganglioma located over the thyroid cartilage in the midline is reported. the tumor had all the appearances of a carotid body tumor, but its odd location could not be explained on the basis of previously known areas where paraganglionic tissues have been found. since a paraganglioma has also hitherto been described within the thyroid gland, clinicians and pathologists alike are cautioned that unusually located paragangliomas may be expected in the future.	2003	168958
regulation of sensitivity to beta-adrenergic stimulation in induction of pineal n-acetyltransferase.	stimulation by isoproterenol causes large increases in the activity of rat pineal n-acetyltransferase (arylamine acetyltransferase or acetyl-coa:arylamine n-acetyltransferase, ec 2.3.1.5) after a variable lag period. lengthening periods of exposure to light cause rapid increases in the sensitivity of pineal n-acetyltransferase to induction by isoproterenol. this supersensitivity, which develops gradually over a 12 hr period, is correlated with increasingly longer lag periods in the induction of ...	1975	168570
the immune response to infection with vaccinia virus in mice. i. infection and the production of antibody neutralizing cell-associated and cell-free virus.	the onset, duration and magnitude of antibody responses to a poxvirus infection were examined. mice were inoculated intravenously with the wr strain of vaccinia virus and developed pocks on their tails. the number of pocks was related to the size of the inoculum. virus was detectable in the spleen and infected mice were subsequently immune to intravenous and intra-nasal challenge. sera of infected animals neutralized both cell-free and cell-associated virus. antibody against cell-free virus appe ...	1975	168248
improvements in the thin-layer chromatography of natural products. i. thin-layer chromatography of the aflatoxins.	during the period which has elapsed since the aflatoxins were first isolated, one of the main problems has been the separation of the individual aflatoxins in pure form from aflatoxin-containing extracts. this separation has been best effected by thin-layer chromatography, and in this paper we describe how some of the difficulties may be overcome by using an appropriate combination of solvent system and silica gel preparation. for the examination of aflatoxin-containing extracts from the mycelia ...	1975	168220
a possible non-oncogenic effect of c type bone cell virus on serum calcium levels of potentially leukemic mice.	in bone of c3h/fg mice, particles structurally identical to c-type leukemia virus arise from membranes of osteocytes and osteoblasts. although these virus apparently do not induce morphologic or neoplastic change in bone they may have other, more subtle, effects. thus, comparison of sera from male c3h/fg mice, a high leukemia-prone strain, with c57bl and c3h/hej mice, low leukemia strains which do not contain c-type virus in bone, reveals that serum calcium levels are significantly lower in the ...	1996	167915
differential effects of lipid depletion on membrane sodium-plus-potassium ion-dependent adenosine triphosphatase and potassium ion-dependent phosphatase.	the phospholipid-dependence of the (na-++k-+)-dependent atpase (adenosine triphosphatase) (ec 3.6.1.3) and associated k-+-dependent phosphatase activity (ec 3.6.1.7) have been compared. unlike the (na-++k-+)-dependent atpase activities, the k-+-dependent phosphatase activities of a number of different preparations were not closely correlated with their total phospholipid contents. after partial lipid depletion with a single extraction in lubrol w the residual atpase and phosphatase activities we ...	1975	167727
the effect of arginine deprivation on the cytopathogenic effect and replication of human cytomegalovirus.	arginine is necessary for the development of the cytopathogenic effect of human cytomegalovirus in human embryonic fibroblasts. it is also required, though in greater concentrations, for the production of infective virions, the requirement being at an early stage of replication. inhibitor studies suggested that this block in replication caused by arginine deficiency was prior to the formation of viral dna. withdrawal of arginine from the medium 24 or 48 hours after infection resulted in a declin ...	1975	167690
respiratory activity of ehrlich ascites tumour cell nuclei.	1. intact ehrlich ascites tumour cells exhibit a residual respiration which is insensitive to site-specific inhibitors of mitochrondrial electron transport. treatment of the tumour-bearing mice with phenobarbital does not influence the rate of this inhibitor-resistant respiration. 2. among the respiratory pigments analysed spectrophotometrically in the microsomal fraction prepared from ascites cells, only small amounts of flavoproteins (nadh-cytochrome b5 reductase and nadph-cytochrome c reducta ...	1995	167532
newer knowledge in comparative virology--its contribution to human health research.	like other comparative sciences, and despite its recent beginning comparative virology has already contributed useful applications and observations to human health research. teachings derived from the study of marek's disease found application in that of burkitt's lymphoma, and may lead to a possible vaccine against the human disease. equally useful information came from the study of canine distemper in the development of a chorio-allantoic membrane attenuated measles vaccine, and in our knowled ...	1975	167504
[glomus tumor of the vagus nerve].		1995	167496
interferon production in mice by the capsular polysaccharide of klebsiella pneumoniae.	the capsular polysaccharide of klebsiella pneumoniae (cps-k) type 1, kasuya strain, induces interferon production in the blood of mice when injected intravenously. cps-k resembles bacterial endotoxin (lipopolysaccharide) in the time pattern of interferon production, with peak levels 2h after injection. cps-k on a weight basis exhibits a more potent interferon-inducing effect than lipopolysaccharide. the active substance responsible for the interferon-inducing activity of cps-k is the neutral cps ...	1975	166921
purification and properties of moniezia cytochrome c550.		1975	166788
chemotherapeutic nitroheterocycles. 18. 2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups.	2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups and related compounds (41-69, table ii) were synthesized and their antimicrobial activities were evaluated (table iii). some of these compounds (e.g. 47, 52, and 59) suprisingly exhibited a broad antibacterial spectrum including proteus species and pseudomonas aeruginosa. extraordinary antitrichomonal activities could also be observed in vitro (mic of compound 59, 0.0004 pg/ml) and ...	1975	166185
the influence of sodium, potassium and lanthanum on amino acid release from spinal-medullary synaptosomes.		1975	166144
assignment of the configuration of the steroid spin label, 3-doxyl-5alpha-cholestane.		2003	166109
properties of improved glass-ionomer cement formulations.		2003	166096
separation of tetracyclines by high-speed liquid chromatography.	the separation of tetracycline and its four commoninpurities has been studied by high-speed liquid chromatography. a preliminary study of the effectiveness of ion-exchange, adsorption, liquid-liquid partition and reversed-phase ion-pair chromatography indicated that only the last method showed promise. by developing special hydrocarbon-bonded stationary phases a rapid and complete resolution of all five tetracyclines has been obtained within 10 min. plate heights using a derivatised 18-mum parti ...	2003	166085
improved thin-layer chromatography system for methadone and its metabolites in biological samples.		2003	166082
the crystal structure of bonito (katsuo) ferrocytochrome c at 2.3 a resolution. ii. structure and function.	the structure analysis of bonito heart ferrocytochrome c was carried out at 2.3 a resolution by x-ray diffraction, and a kendrew-type skeletal model was built up. this molecule has an overall egg shape, 35 a in height, 30 a in width and 23 a in thickness; the 5th ligand of the heme iron atom is the n-epsilon atom of the his-18 imidazole ring and the 6th is the met-80 sulfur atom. distinct alpha-helix regions are found between the n-terminus and reside 11, between 60 and 69, and between 90 and th ...	1975	166072
the ultrastructure of the sensory end-organs (baroreceptors) in the atrial endocardium of young mini-pigs.	light and electron microscopical studies on pig hearts have confirmed the presence in the right and the left atrial endocardium of distinct circumscribed, unencapsulated end-organs (baroreceptors) associated with 4-9 mum thick nerve fibres. the myelin sheath terminates before the entrance of the nerve into the end-organ. the regular presence of thin axon profiles containing clusters of small agranular vesicles, both around the thick nerve fibre and in the periphery of the end-organs, suggests a ...	1975	166065
adenovirus infection in two calves.	two 2-week-old calves with pyrexia, diarrhea, and subsequent dehydration were treated symptomatically for 1 week, but without favorable response. at necropsy, foci of necrosis were found in the abomasum and rumen of each calf. the small and large intestineswere dilated by grayish, turbid fluid. numerous large amphophilic, intranuclear inclusions in endothelial cells of blood vessels in the abomasum and rumen, in endothelial cellsof the adrenal cortical sinusoids and renal glomeruli, and in intes ...	1975	166059
results of radiation treatment of naturally occurring animal tumors.		1975	166056
a simple, specific, radioisotopic assay for 5'-nucleotidase.		2003	165748
glucagon and adenylate cyclase: binding studies and requirements for activation.	solubilization of myocardial adenylate cyclase abolished responsiveness to glucagon and catecholamines, two of the hormones which activate the membrane-bound enzyme. adenylate cyclase freed of detergent by deae-cellulose chromatography continues to remain unresponsive to hormone stimulation. however, adding purified bovine brain phospholipids--phosphotidylserine and monophosphatidylinositol--restored responsiveness to glucagon and catecholamines, respectively. 125-i-glucagon binding appeared to ...	1975	165684
letter: light induced change in rod outer segment membrane fluidity.		1975	165628
immunoreactive thyrotrophin releasing factor in gastropod circumoesophageal ganglia.		2003	165422
a sensitive bioassay for the determination of human plasma acth levels.	a sensitive bioassay for the measurement of plasma acth is presented. the use of silicic acid adsorption of plasma, with a subsequent acid wash and aqueous acetone desorption, was successful in removing those substances which had interfered with the steroidogenic response of dispersed adrenal cells when unextracted plasma was employed. this extraction procedure extracted 72-76% of acth present in plasma. two pg acth1-39 could be consistently detected. alpha-hacth1-39 and alpha-pacth1-39 exhibite ...	1975	165419
thyroid-stimulating hormone and cyclic adenosine 3',5'-monophosphate in the regulation of thyroid gland function.	the initial step in tsh action reflects binding of the hormone to specific receptor sites on the plasma membrane. such binding has been studied using plasma membranes, homogenates, isolated thyroid cells grown in culture, and thyroid slices. 3-h- and iodinated tsh preparations have been used; the latter have been prepared using both chloramine-t and lactoperoxidase. some of the discrepancies reported in the literature might reflect the different thyroid and hormone preparations and the variable ...	1975	165359
studies on cyclic amp metabolism in human epidermoid carcinoma (hep-2) cells.	cultured human epidermoid carcinoma (hep-2) cells were found to contain a highly responsive, catecholamine-sensitive adenyl cyclase activity in cellfree preparations. by contrast, cyclic amp levels in intact hep-2 cells were at best only marginally increased by catecholamines under a variety of conditions. the lack of an intact cell response could not be accounted for by escape of cyclic amp to the medium, excessive phosphodiesterase activity, inactivation of the catecholamine, or by unusual kin ...	1975	165357
sterol carrier protein hypothesis: requirement for three substrate-specific soluble proteins in liver cholesterol biosynthesis.		2003	165341
studies on a transplantable murine rhabdomyosarcoma.	well-differentiated rhabdomyosarcomas developed in 8 of 10 balb/c mice inoculated with cell-free extracts of two lesions arising in bats previously inoculated with the moloney strain of murine sarcoma virus. the tumors could be transplanted in balb/c mice and other strains of mice compatible at the h-2 locus. a tumorigenic clonal cell culture line (r2) was established from a transplanted tumor. ultrastructural studies of the primary and transplanted tumors and r2 cells revealed thick and thin my ...	2003	165312
vertical transmission of c-type viruses: their presence in baboon follicular oocytes and tubal ova.	c-type viruses were found in baboon follicular oocytes and tubal ova adjacent to the plasma membrane in the perivitelline space or along the inner margin of the zona pellucida. their presence support the concept of vertical transmission of c-type viruses.	2003	165309
vestibular nystagmus and teleost oculomotor neurons: functions of electrotonic coupling and dendritic impulse initiation.	1. nystagmus in the horizontal plane is evoked in fish by mechanical stimulation of the ampulla of the horizontal semicircular canal or by electrical stimulation of the nerve from this canal. the movements are conjugate and the slow phase is away from the side of stimulation. 2. medial rectus motoneurons were recorded from intracellularly, during nystagmus. during the slow phase (induced by ipsilateral stimulation), impulses arise abruptly from the base line and appear to arise at a distance fro ...	2003	165274
analysis of postural motoneuron activity in crayfish abdomen. ii. coordination by excitatory and inhibitory connections between motoneurons.	the identified spike trains of the crayfish abdominal postural efferent neurons were recorded simultaneously from one or more segments. the efferent activity was analyzed using cross-correlation histograms, peristimulus time scatter diagrams, and specialized antidromic techniques. the analyses show that the larger, phasically active motoneurons are coordinated in their activity by cross connections made at the motoneuron level. the cross connections are both excitatory and inhibitor in nature an ...	2003	165271
receptive fields of cerebellar cells receiving exteroceptive input in a gymnotid fish.	single neurons in the caudal lobe of the cerebellum of the weakly electric fish apteronotus albifrons respond to distortions in the normal electric field produced by the animal. moving plastic or metal objects as well as a simpler stimulus, a moving electrical dipole, produce adequate distortions of the fish's field to cause the cerebellar cells to respond. the moving dipole stimulated small enough areas of the fish's skin, as determined by the responses of single electroreceptors, to allow maps ...	2003	165269
local and systemic response after simultaneous intranasal inoculation of temperature-sensitive mutants of parainfluenza 3, ibr and bovine adenovirus 3.	triple seronegative calves were exposed by the nasal route to three (ts) mutants of bovine respiratory viruses (pi3, ibr, adeno3). after a single exposure, they responded with significant levels of serum antibodies to the three viruses. nasal antibodies were demonstrated for pi3 and adenovirus antigens. the failure to demonstrate nasal antibodies to ibr may be due to lack of sensitivity of the procedure used. when reexposed six weeks later, calves had sharp increases in levels of serum antibodie ...	1975	165126
extramammary paget's disease and anaplastic basaloid small-cell carcinoma of the anus: report of a case.		2003	165051
intranuclear and cytoplasmic annulate lamellae in grasshopper spermatocytes (genus melanoplus).	intranuclear and cytoplasmic annulate lamellae were studied in grasshopper spermatocytes (melanoplus) with the electron microscope. although cytoplasmic annulate lamellae were observed in all three species examined, intranuclear annulate lamellae were found in only one species. the intranuclear annulate lamellae encompass certain nuclear material adjacent to the nuclear envelope forming a vesicle that is extruded into the spermatocyte cytoplasm. in this same species, cytoplasmic annulate lamella ...	2003	164286
[isolation of the virus of transmissible gastroenteritis of swine from cell cultures and antigenic comparison of 2 american strains].		1975	164273
the attack mechanism of an exo-1,3-beta-glucosidase from basidiomycete sp. qm 806.	the attack mechanism of a purified exo-1,3-beta glucosidase (1,3-beta-d-glucan glucohydrolase, ec 3.2.1.58) was investigated by using as a substrate a mixture of two structurally characterized periodate-oxidized and reduced unbranched 1,3-beta-d-glucans which differed only at the reducing terminal. the substrates, derivatives of laminarin, were altered only at the terminals due to resistance of the internal (1 leads to 3)-linked glucosyl residues to periodate oxidation. each glucan has only a si ...	2003	164221
the biogenesis of mitochondrial membranes in the yeast saccharomyces cerevisiae.	membrane lipids of yeast mitochondria have been enriched by growing yeast cells in minimal medium supplemented with specific unsaturated fatty acids as the sole lipid supplement. using the activity of marker enzymes for the outer (kynurenine hydroxylase) and inner (cytochrome c oxidase and oligomycin-sensitive atpase) mitochondrial membranes, arrhenius plots have been constructed using both promitochondria and mitochondria obtained from o2-adapting cells in the presence of a second unsaturated f ...	1975	164216
the synthesis and turnover of virus-specific polyadenylated rna in polyoma-infected cells.		1975	164192
the influence of age of cell cultures on the production of foot-and-mouth disease antigen. brief report.		1975	164159
bioenergetics of mitochondria from flight muscles of aging blowflies: partial reactions of oxidation and phosphorylation.		1997	164153
progesterone-binding proteins: in vitro binding and biological activity of different steroidal ligands.	progesterone-binding proteins from human, rabbit, sheep and guinea pig myometrial cytosol, all induced with oestradiol, as well as from pregnant guinea pig myometrium and plasma were investigated. the physico-chemical properties of the oestradiol-induced binding proteins were very similar in all the species studied. in all, 63 steroids were tested for their ability to compete with tritiated progesterone for the binding sites on these six proteins and their relative affinities were determined. th ...	1975	164094
androgen-induced nuclear accumulation of the estrogen receptor.	the effect of androgens on the nuclear uptake of both tritiated estradiol (3h-e2) and the estrogen receptor was studied in immature rat uteri. it was demonstrated that in vitro preincubation of immature rat uteri with various androgens (1 mum to 50 mum) followed by incubation with 3h-e2 (20 nm) resulted in a greatly decreased specific nuclear uptake of 3h-e2. non-androgenic steroids had no effect. it was also confirmed that 5alpha-dihydrotestosterone (dht) causes the accumulation of the estrogen ...	1975	164064
genetic heterogeneity in xeroderma pigmentosum: complementation groups and their relationship to dna repair rates.	fibroblast strains from 12 patients with xeroderma pigmentosum had lower than normal rates of dna repair, as determined by autoradiographic studies of ultraviolet-induced unscheduled nuclear dna synthesis. the nuclei in binuclear cells, obtained by fusing fibroblasts from certain pairs of these strains, had a greater rate of dna repair than the nuclei of either strain's unfused mononuclear cells. these results indicate that complementary corrections of the strains' repair defects had occurred in ...	1975	164028
the antagonistic effect of an inhalation anesthetic and high pressure on the phase diagram of mixed dipalmitoyl-dimyristoylphosphatidylcholine bilayers.	several workers have shown that phase transition-related changes in membrane lipids have a profound effect on membrane-solvated protein function. this phase transition temperature dependence has been explained as resulting from the formation of lateral phase separations within the membrane bilayer. the present study demonstrates that a clinical concentration of an inhalation anesthetic produces changes in both the phase transition temperature of pure lipid bilayers and the lateral phase separati ...	1997	164016
radioreceptor assay for human chorionic gonadotropin.	a sensitive radioreceptor assay capable of detecting 100 pg of human chorionic gonadotropin has been developed using ovarian receptors and (125i)hcg. the dose-response curves of a series of urine and serum samples from pregnant women were linear and parallel to standard curves of 2nd international standard hcg and purified hcg when logit of percent bound was plotted against log dose. potency estimates of pregnancy sera, measured by radioreceptor assays, were approximately 2 times greater than th ...	1975	163971
collagen in the human lung. quantitation of rates of synthesis and partial characterization of composition.	the presence of collagen in lung is fundamental in normal lung structure and function. methods have been developed to examine human fetal and adult lung collagen with respect to its composition and synthesis. the second trimester fetal lung has a large number of cells per unit lung mass (36.6 plus or minus 2.7 mug dna/mg dry wt) and relatively small amounts of collagen (17.0 plus or minus 5.3 mug collagen/mg dry wt). the number of cells per unit lung mass in the adult lung (11.1 plus or minus 3. ...	1975	163849
prevention of gram-negative bacillary pneumonia using polymyxin aerosol as prophylaxis. ii. effect on the incidence of pneumonia in seriously ill patients.	all 744 patients admitted to a respiratory-surgical intensive care unit (rsicu) were included in a prospective study of the effects of a polymyxin (2.5 mg/kg body wt/day in six divided doses) or a placebo aerosol sprayed into the posterior pharynx and tracheal tube (if present), during 11 alternating 2-mo treatment cycles. the incidence of upper airway colonization in the rsicu with pseudomonas aeruginosa was 1.6% during the polymyxin treatment cycles (total 374 patients) and 9.7% during the pla ...	1975	163848
notochordal stimulation of in vitro somite chondrogenesis before and after enzymatic removal of perinotochordal materials.		1997	163775
potentiation of the effect of histamine by pge2 in the isolated perfused rabbit kidney and guinea-pig lung.		1975	163751
benzo(alpha)pyrene effects on mouse epithelial cells in culture.	the effect of benzo (a) pyrene on the growth in culture of 5 mouse epithelial cell strains was examined. these epithelial cells are highly sensitive to the cytotoxic action of benzo (a)-pyrene. in addition, the activity of the benzol (a) pyrene-metabolizing system, aryl hydrocarbon hydroxylase, is low but highly iducible by the carcinogen. as the sensitivity of a cell strain to the cytotoxic action of benjo (a) pyrene decreased, the inudcibility of the hydroxylase also decreased,. however, a str ...	1975	163692
adrenocortical activity and urinary cyclic amp levels: effects of hypobaric hypoxia.	six highly motivated and trained military test subjects were exposed to a simulated altitude of 4267 m (477 torr) for 48 hours, prededed and followed by sea level runs lasting 32 hours. during each scenario tests subjects were required to perform their respective military tasks on a continual basis with sporadic rest not exceeding several hours. extremely high levels of plasma cortisol prior to the start of each session and persistently elevated concentrations of urinary 17-oh corticosteroids at ...	1996	163634
microbial assimilation of hydrocarbons. i. the fine-structure of a hydrocarbon oxidizing acinetobacter sp.	1. the fine-structure analysis of the hydrocarbon oxidizing microorganism, acinetobacter sp., demonstrated a cytoplasmic modification resulting from growth on paraffinic and olefinic hydrocarbons. 2. intracytoplasmic hydrocarbon inclusions were documented by electron microscopy with chemical identifications obtained by gas chromatography and x-ray diffraction. 3. these results demonstrate the ability of a microorganism to accumulate hydrocarbon substrates intracellularly which, in turn, indicate ...	1975	163624
[surgical short-term treatment].		1996	163420
[study trip of the german nursing association to south africa; from oct. 30 - nov. 11, 1974].		1996	163408
evaluation of dye exclusion and colony inhibition techniques for detection of polyoma-specific, cell-mediated immunity.	cellular immunity directed against polyoma virus-induced antigen was observed with c3h/hej splenic lymphoid cells from mice sensitized by a short-term immunization schedule with syngeneic polyoma 4198 and 4198v tumor cells. polyoma specificity of the response was shown by demonstration that splenic cells from dba/2j animals with polyoma virus-induced tumors were cytotoxic for the c3h 4198 and 4198v cells, but not for the l-m cell, another cell line of c3h origin. the polyoma-specific response in ...	1975	163318
uptake of 3-o-methyl-d-glucose into cultured human glioma cells.		1975	163292
radiolabeling of proteins and viruses in vitro by acetylation with radioactive acetic anhydride.	we describe a convenient, rapid, and reproducible method for labeling proteins in vitro by acetylation with [3h] or [14-c]acetic anhydride dissolved in small amounts of anhydrous dioxane. the reaction is carried out at neutral ph and does not require the use of detergents, water-immiscible organic solvents, oxidizing, or reducing agents. thus undesirable solvent-induced alterations in protein structure and biological activity are minimized. a method for calculating the specific activity of the p ...	1975	163253
metabolism, function and clinical aspects of vitamin d.	briefly reviewed herein are some of the contemporary findings on the metabolism of vitamin d, and the biochemical and physiological effects of this steroid in the animal. certainly the most accepted major action of vitamin d is to enhance the intestinal absorption of calcium. historically, there is also considerable evidence that the vitamin d is required for the resorption of calcium from bone, thereby aiding in maintaining normal serum calcium levels. increasing evidence is becoming available ...	1975	163168
editorial: treatment for cataplexy.		1996	163113
antiviral drugs for the neonate--the risk-benefit ledger.		1975	163110
cyclic amp and cyclic gmp content and binding in malignancy.		1975	163087
methods for assessing steroid-receptor effect on rna synthesis in isolated nuclei.		1975	162990
classification of enterotoxins on the basis of activity in cell culture.	two cell culture systems were used in a study of the biological properties of several bacterial enterotoxins in vitro. by means of one model, in which hela cell monolayers were used, cytotoxic effects, interms of detachment of cells from a glass surface due to cell death, were assayed. by means of the second model, activation of the adenyl cyclase-cyclic adenosine 3', 5'-monophosphate (amp) system, in terms of increased steroidogenesis by y-1 adrenal cells (an effect which we have termed cytoton ...	1975	162926
ultrastructural studies of parallel tubular arrays in human lymphocytes.	parallel tubular inclusions were found in peripheral blood lymphocytes from 18 patients with various hematologic disorders, primarily lymphoproliferative processes, and 1 apparently healthy individual. the inclusions varied in size from 1000 to 6000 a and were usually membrane bounded. the microtubule-like structures comprising the inclusions ranged in size from 150 to 300 a and were packed in wall-to-wall contact with each other. dense amorphous material and small dark crystalloids were frequen ...	1975	162823
hemoperitoneum from liver cell adenoma in a patient on oral contraceptives.	a case of hemoperitoneum from ruptured benign liver cell adenoma in a young woman with a long history of oral contraceptive use is presented. the importance of recognizing the cause of this intra-abdominal emergency is stressed, and comment is made about the entity occurring in women on contraceptive drugs.	1995	162813
says cme article contained certain incomplete information.		1995	162694
heparin insensitivity responding to fresh frozen plasma.		1995	162689
lumbar discography. its value in diagnosis and treatment of lumbar disc lesions.	lumbar discography has been performed in over 1500 patients at st. luke's episcopal hospital, and a report concerning 683 cases has been previously published. the authors review an additional 199 cases, finding that decision making was influenced by discography in 155 cases (78%). a positive discogram was surgically confirmed in 111 patients (56%). in 14(7%) the disc was found to be normal at surgery. one hundred six patients (53%) had positive discograms with negative or equivocal myelogram. in ...	1995	162547
isolation and characterization of nuclei from neurospora crassa.	a procedure was developed for isolating nuclei from either the conidial or germinated conidial growth phase of neurospora crassa. a frozen conidial suspension was lysed by passage through a french pressure cell, and the nuclei were freed from the broken cells by repeated homogenization in an omni-mixer. pure nuclei were obtained from the crude nuclear fraction by density banding in a ludox gradient. the final nuclear yield was 20 to 30%. the nuclei had a deoxyribonucleic acid (dna):ribonucleic a ...	1995	162536
experimental enzyme evolution.		1979	162365
[scleroma].		1979	162348
atp-ase activity and lipid content of erythrocytes in treatment of acute lymphoblastic leukemia in children.	in erythrocytes of children with the acute lymphoblastic leukemia the rise in total phospholipid content, confined predominantly to lecithin, the decrease of cholesterol/phospholipid ratio and the increase of the mg++-activated atp-ase activity was found prior to treatment. the abnormalities of the lipid composition were in general persistent also in remission. the activity of erythrocytes atp-ase decreased to normal values after achievement of the reversion of clinical and hematological symptom ...	2003	162034
[first findings with sr-isosit-pe crown and bridge material].		2003	162023