Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| transfer between an algerian and a french hospital of four multi-drug resistant bacterial strains together via a single patient. | a 5 years-old girl, seriously burnt with fire, was first hospitalized during four days in an hospital at alger, and then transferred to our hospital at paris. admitted in our intensive care burns unit, she was third degree burnt on 78% of total body surface area, already treated with imipenem and vancomycin at her arrival. clinical aggravation was rapidly observed and death occurred within 24 hours. cultures of blood and multiple wound swabs yielded 3 multi-drug resistant bacterial strains: acin ... | 2015 | 26550534 |
| identification and characterization of ctx-m-15 producing klebsiella pneumoniae clone st101 in a hungarian university teaching hospital. | we investigated the molecular epidemiology of extended spectrum β-lactamase (esbl) producing klebsiella pneumoniae isolates derived from the teaching hospitals of university of pécs, pécs, hungary in the time period 2004-2008. molecular typing, antimicrobial susceptibility testing, detection of common β-lactamase genes (bla(ctx-m), bla(tem) and bla(shv)) and virulence associated traits (hypermucoviscosity, maga, k2a, rmpa, siderophores, type 1 and 3 fimbria, biofilm formation, serum resistance) ... | 2015 | 26551567 |
| clinical manifestations of pneumonia according to the causative organism in patients in the intensive care unit. | whether the causative organism influences the clinical course of pneumonia in the intensive care unit (icu) is controversial. we assessed the clinical manifestations and prognosis of pneumonia according to the causative pathogens in patients in a medical icu. | 2015 | 26552458 |
| molecular epidemiology of carbapenem resistant enterobacteriaceae in valle d'aosta region, italy, shows the emergence of kpc-2 producing klebsiella pneumoniae clonal complex 101 (st101 and st1789). | the spread of carbapenem resistant enterobacteriaceae (cre) is an emerging clinical problem, of great relevance in europe and worldwide. the aim of this study was the molecular epidemiology of cre isolates in valle d'aosta region, italy, and the mechanism of carbapenem resistance. | 2015 | 26552763 |
| ertapenem-containing double-carbapenem therapy for treatment of infections caused by carbapenem-resistant klebsiella pneumoniae. | we describe outcomes of patients with infections with carbapenem-resistant klebsiella pneumoniae (crkp) who received ertapenem-containing double-carbapenem therapy (ecdct). clinical success was observed in 7/18 (39%) patients overall: bloodstream infections, 3/7 (43%); pneumonia, 1/5 (20%); intraabdominal infections, 0/2 (0%); urinary tract infections, 2/3 (67%); and a skin and skin structure infection, 1/1 (100%). microbiologic success was observed in 11/14 (79%) evaluable patients; 5/18 (28%) ... | 2015 | 26552970 |
| prevalence of resistant gram-negative bacilli in bloodstream infection in febrile neutropenia patients undergoing hematopoietic stem cell transplantation: a single center retrospective cohort study. | bloodstream infection (bsi) is an important cause of morbidity and mortality in patients undergoing hematopoietic stem cell transplantation (hsct). to evaluate the causative bacteria and identify risk factors for bsi associated mortality in febrile neutropenia patients undergoing hsct, we collected the clinical and microbiological data from patients underwent hsct between 2008 and 2014 and performed a retrospective analysis. throughout the study period, among 348 episodes of neutropenic fever in ... | 2015 | 26559260 |
| antibacterial activity of achievable epithelial lining fluid exposures of amikacin inhale with or without meropenem. | while amikacin inhale (bay41-6551), an integrated drug-device combination under development, achieves an estimated amikacin epithelial lining fluid (elf) concentration of ∼ 5000 mg/l, its target site pharmacodynamics are unknown. we evaluated the pharmacodynamics of elf exposure of inhaled amikacin ± meropenem. | 2016 | 26559690 |
| a trial with igy chicken antibodies to eradicate faecal carriage of klebsiella pneumoniae and escherichia coli producing extended-spectrum beta-lactamases. | extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae is an emerging therapeutic challenge, especially in the treatment of urinary tract infections. following an outbreak of ctx-m-15 klebsiella pneumoniae in uppsala, sweden, an orphan drug trial on igy chicken antibodies was undertaken in an attempt to eradicate faecal carriage of esbl-producing k. pneumoniae and escherichia coli. | 2015 | 26560861 |
| effects of space environment on genome, transcriptome, and proteome of klebsiella pneumoniae. | the aim of this study was to explore the effects of space flight on klebsiella pneumoniae. | 2015 | 26562174 |
| [clinical impact of infections with carbapenem-resistant enterobacteriaceae in liver transplant recipients]. | infections with carbapenem-resistant enterobacteriaceae (cre) are an emerging cause of morbidity and mortality among liver transplant recipients (ltr) worldwide, particularly klebsiella pneumoniae carbapenemase (kpc)-producing organisms. approximately 3 - 13 % of solid organ transplant recipients in cre-endemic areas develop cre infections, and the infection site correlates with the transplanted organ. the cumulative 30-day mortality rate of ltr infected with carbapenem-resistant k. pneumoniae i ... | 2015 | 26562402 |
| genome sequence of a clinical klebsiella pneumoniae sequence type 6 strain. | we report here the genome sequence of klebsiella pneumoniae ch1034, a sequence type 6 (st6) strain isolated in 2012 from a central venous catheter of a hospitalized patient. | 2015 | 26564039 |
| complete genome sequence of klebsiella pneumoniae strain hkuoplc, a cellulose-degrading bacterium isolated from giant panda feces. | we report here the complete genome sequence of klebsiella pneumoniae strain hkuoplc, isolated from a giant panda fecal sample collected from ocean park, hong kong. the complete genome of this bacterium may contribute to the discovery of efficient cellulose-degrading pathways. | 2015 | 26564041 |
| binary interactions of antagonistic bacteria with candida albicans under aerobic and anaerobic conditions. | we used both aerobic and anaerobic liquid co-cultures, prepared with luria bertani broth, to study the effect of bacteria on the survival of candida albicans in the external environment, away from an animal host. the bacteria were represented by aeromonas hydrophila, bacillus cereus, bacillus subtilis, clostridium, enterobacter, klebsiella pneumoniae, kluyvera ascorbata and serratia marcescens. under aerobic conditions, the yeast's growth was inhibited in the presence of bacterial growth; howeve ... | 2016 | 26566932 |
| erratum. | simkins, j, aragon, l, fan, j, frederick, c, camargo, j. f. intravenous fosfomycin treatment for carbapenem-resistant klebsiella pneumoniae in the united states. ann pharmacother. 2015;49:1177-1178 (original doi: 10.1177/1060028015598326).on page 1177, dr. jose f. camargo is listed as the 3(rd) author. however, he should be the last author in the sequence. | 2015 | 26567262 |
| clinical characteristics of carbapenem-resistant klebsiella pneumoniae infections in ill and colonized children in colombia. | multidrug-resistant gram-negative infections represent a growing problem and a serious global threat. data in children are scarce. klebsiella pneumoniae carbapenemases (kpc) are the most common mechanism of resistance this organism has developed. we report the clinical characteristics and outcomes from a cohort of children infected or colonized with carbapenem-resistant k. pneumoniae (crkp) at a tertiary care center in medellín, colombia. | 2016 | 26569194 |
| identification and characterization of imipenem-resistant klebsiella pneumoniae and susceptible klebsiella variicola isolates obtained from the same patient. | klebsiella variicola, a bacterium closely genetically related to klebsiella pneumoniae, is commonly misidentified as k. pneumoniae by biochemical tests. to distinguish between the two bacteria, phylogenetic analysis of the rpob gene and the identification of unique genes in both bacterial species by multiplex-polymerase chain reaction (pcr) provide the means to reliably identify and genotype k. variicola. in recent years, k. variicola has been described both as the cause of an intrahospital outb ... | 2016 | 26571390 |
| [a case of metastatic endophthalmitis resulting from liver abscess complicated with pyogenic ventriculitis via optic nerve]. | intracranial extension of infection via the optic nerve is a rare but serious complication of bacterial endophthalmitis. | 2015 | 26571629 |
| cefazolin loaded chitosan nanoparticles to cure multi drug resistant gram-negative pathogens. | antibiotic resistance against gram-negative microbes is considered as an alarming phenomenon that needs to be addressed urgently to develop better therapeutic solutions. the aim of the present research work was to investigate and develop cefazolin loaded chitosan nanoparticles (csnps) as a potential tool against multidrug resistant pathogens. empty and drug loaded csnps were prepared by ionic gelation method. it was observed by scanning electron microscopy (sem) and atomic force microscopy (afm) ... | 2016 | 26572401 |
| comment on "production of 2-butanol from crude glycerol by a genetically-engineered klebsiella pneumoniae strain [oh et al., biotechnol lett (2014) 36:57-62]". | 2016 | 26573636 | |
| emergence of carbapenem-resistant serotype k1 hypervirulent klebsiella pneumoniae strains in china. | we report the emergence of five carbapenem-resistant k1 hypervirulent klebsiella pneumoniae (hvkp) strains which caused fatal infections in hospital patients in zhejiang province, china, upon entry through surgical wounds. genotyping results revealed the existence of three genetically related strains which exhibited a new sequence type, st1797, and revealed that all strains harbored the maga and wcag virulence genes and a plasmid-borne bla(kpc-2) gene. these findings indicate that k1 hvkp is sim ... | 2015 | 26574010 |
| in vitro antimicrobial activity of a siderophore cephalosporin, s-649266, against enterobacteriaceae clinical isolates, including carbapenem-resistant strains. | s-649266 is a novel siderophore cephalosporin antibiotic with a catechol moiety on the 3-position side chain. two sets of clinical isolate collections were used to evaluate the antimicrobial activity of s-649266 against enterobacteriaceae. these sets included 617 global isolates collected between 2009 and 2011 and 233 β-lactamase-identified isolates, including 47 kpc-, 49 ndm-, 12 vim-, and 8 imp-producers. the mic90 values of s-649266 against the first set of escherichia coli, klebsiella pneumo ... | 2016 | 26574013 |
| nosocomial bloodstream infections caused by escherichia coli and klebsiella pneumoniae resistant to third-generation cephalosporins, finland, 1999-2013: trends, patient characteristics and mortality. | few systematically collected multi-centre surveillance data on nosocomial bloodstream infections (bsi) caused by extended-spectrum β-lactamase (esbl) producing escherichia coli or klebsiella pneumoniae have been published. | 2016 | 26577519 |
| influence of biological fluids in bacterial viability on different hospital surfaces and fomites. | the hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. the present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces commonly present in nosocomial environment. | 2016 | 26577628 |
| complete genome sequence of novel t7-like virus vb_kpnp_kpv289 with lytic activity against klebsiella pneumoniae. | a novel bacteriophage, vb_kpnp_kpv289, lytic for hypermucoviscous strains of klebsiella pneumoniae, was attributed to the family podoviridae, subfamily autographivirinae, genus t7likevirus based on transmission electron microscopy and genome analysis. the complete genome of the bacteriophage vb_kpnp_kpv289 consists of a linear double-stranded dna of 41,054 bp including 179-bp direct-repeat sequences at the ends and 51 open reading frames (orfs). the g+c content is 52.56 %. the phage was shown to ... | 2016 | 26577901 |
| deciphering tissue-induced klebsiella pneumoniae lipid a structure. | the outcome of an infection depends on host recognition of the pathogen, hence leading to the activation of signaling pathways controlling defense responses. a long-held belief is that the modification of the lipid a moiety of the lipopolysaccharide could help gram-negative pathogens to evade innate immunity. however, direct evidence that this happens in vivo is lacking. here we report the lipid a expressed in the tissues of infected mice by the human pathogen klebsiella pneumoniae. our findings ... | 2015 | 26578797 |
| s-thanatin functionalized liposome potentially targeting on klebsiella pneumoniae and its application in sepsis mouse model. | s-thanatin (ts) was a short antimicrobial peptide with selective antibacterial activity. in this study, we aimed to design a drug carrier with specific bacterial targeting potential. the positively charged ts was modified onto the liposome surface by linking ts to the constituent lipids via a peg linker. the benefits of this design were evaluated by preparing a series of liposomes and comparing their biological effects in vitro and in vivo. the particle size and zeta potential of the constructed ... | 2015 | 26578959 |
| the impact of carvacrol on ammonia and biogenic amine production by common foodborne pathogens. | the impact of carvacrol at different levels (0.1%, 0.5%, and 1%) on ammonia (amn) and biogenic amines (bas) production by 8 common foodborne pathogens (fbps) (staphylococcus aureus, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, pseudomonas aeruginosa, listeria monocytogenes, aeromonas hydrophila, and salmonella paratyphi a) was studied using a rapid high-performance liquid chromatography method. significant differences among bacteria (p < 0.05) in amn and ba production were obs ... | 2015 | 26580308 |
| detection and characterization of carbapenemase-producing enterobacteriaceae in wounded syrian patients admitted to hospitals in northern israel. | since 2013, four hospitals in northern israel have been providing care for syrian nationals, primarily those wounded in the ongoing civil war. we analyzed carbapenemase-producing enterobacteriaceae (cpe) isolates obtained from these patients. isolate identification was performed using the vitek 2 system. polymerase chain reaction (pcr) was performed for the presence of bla kpc, bla ndm, and bla oxa-48. susceptibility testing and genotyping were performed on selected isolates. during the study pe ... | 2016 | 26581423 |
| innate immune signaling activated by mdr bacteria in the airway. | health care-associated bacterial pneumonias due to multiple-drug resistant (mdr) pathogens are an important public health problem and are major causes of morbidity and mortality worldwide. in addition to antimicrobial resistance, these organisms have adapted to the milieu of the human airway and have acquired resistance to the innate immune clearance mechanisms that normally prevent pneumonia. given the limited efficacy of antibiotics, bacterial clearance from the airway requires an effective im ... | 2015 | 26582515 |
| outbreak of kpc-3 producing carbapenem-resistant klebsiella pneumoniae in a us pediatric hospital. | the increase in carbapenem-resistant enterobacteriaceae (cre) infections is a critical public health issue. we recently experienced the largest single-center pediatric outbreak of carbapenem-resistant klebsiella pneumoniae (crkp) at our hospital. the objective of this study was to describe the molecular epidemiology of this outbreak before and after infection-prevention interventions. | 2015 | 26582872 |
| multidrug-resistant escherichia coli and klebsiella pneumoniae isolated from patients in kashan, iran. | escherichia coli and klebsiella pneumoniae are common human pathogens that cause a wide spectrum of infections. antimicrobial resistance is a basic obstacle in the management of these infections which has different patterns in various regions. | 2015 | 26587220 |
| in vitro antibacterial and antibiotic-potentiation activities of the methanol extracts from beilschmiedia acuta, clausena anisata, newbouldia laevis and polyscias fulva against multidrug-resistant gram-negative bacteria. | the present study was designed to investigate the antibacterial activities of the methanol extracts from different parts of beilschmedia acuta kosterm (lauraceae), clausena anisata (willd) hook (rutaceae), newbouldia laevis seem (bignoniaceae) and polyscias fulva (hiern) harms (araliaceae) as well as their synergistic effects with antibiotics against a panel of gram-negative bacteria, including multi-drug resistant (mdr) phenotypes expressing active efflux pumps. | 2015 | 26590804 |
| in vitro activity of cefepime against extended spectrum p-lactamase-producing escherichia coli and klebsiella pneumoniae from clinical specimens at bugando medical centre, mwanza, tanzania. | there is an increase in isolation of extended spectrum beta-lactamase (esbl) producing isolates from clinical samples worldwide. in developing countries the treatment option of esbl producing isolates is limited. recently fourth generation cephalosporins have been introduced for use in tanzania. this study was done to determine in vitro activity of cefepime against esbl producing clinical isolates. disc diffusion testing was performed to 235 esbl producing isolates; of which 73 (31%) were escher ... | 2011 | 26592057 |
| report on a transborder spread of carbapenemase-producing bacteria by a patient injured during euromaidan, ukraine. | spread of carbapenemase-producing bacteria has been described all over the world. this phenomenon may be accelerated by many factors, including wars and natural disasters. in this report, we described an ndm-1-producing klebsiella pneumonia st11 recovered from a patient injured during the maidan revolution in ukraine. to our knowledge, this is the first report of a carbapenemase-producing enterobacteriaceae in ukraine and one of several reports describing wound colonization/infection of humans i ... | 2015 | 26594376 |
| establishment of a multi-species biofilm model to evaluate chlorhexidine efficacy. | chronic infections, for example, diabetic foot ulcers, have a large impact in terms of patient morbidity and mortality. these wounds are characterized by complex polymicrobial communities of bacteria, which may include a number of difficult-to-eradicate multidrug-resistant pathogens. | 2016 | 26597632 |
| carbapenemase-producing klebsiella pneumoniae in romania: a six-month survey. | this study presents the first characterization of carbapenem-non-susceptible klebsiella pneumoniae isolates by means of a structured six-month survey performed in romania as part of an europe-wide investigation. klebsiella pneumoniae clinical isolates from different anatomical sites were tested for antibiotic susceptibility by phenotypic methods and confirmed by pcr for the presence of four carbapenemase genes. genome macrorestriction fingerprinting with xbai was used to analyze the relatedness ... | 2015 | 26599338 |
| emergence of plasmid-mediated colistin resistance mechanism mcr-1 in animals and human beings in china: a microbiological and molecular biological study. | until now, polymyxin resistance has involved chromosomal mutations but has never been reported via horizontal gene transfer. during a routine surveillance project on antimicrobial resistance in commensal escherichia coli from food animals in china, a major increase of colistin resistance was observed. when an e coli strain, shp45, possessing colistin resistance that could be transferred to another strain, was isolated from a pig, we conducted further analysis of possible plasmid-mediated polymyx ... | 2016 | 26603172 |
| epidemiologic and genotypic review of carbapenemase-producing organisms in british columbia, canada, between 2008 and 2014. | carbapenemase-producing organisms (cpos) are a serious emerging problem for health care facilities worldwide. owing to their resistance to most antimicrobial therapies, cpos are difficult to treat and pose a challenge for infection prevention and control. since 2010, lab-based surveillance for cpos and pcr-based testing were implemented in british columbia (bc), canada. a review of cpos in bc from 2008 to march 2014 was done to characterize the resistance mechanisms and possible clonal strain tr ... | 2016 | 26607987 |
| synthesis of novel cellulose carbamates possessing terminal amino groups and their bioactivity. | cellulose phenyl carbonates are an excellent platform to synthesize a broad variety of soluble and functional cellulose carbamates. in this study, the synthesis of cellulose carbamates with terminal amino groups, namely ω-aminoethylcellulose- and ω-aminoethyl-p-aminobenzyl-cellulose carbamate, is discussed. the products are well soluble and their structures can be clearly described by nmr spectroscopy. the cellulose carbamates exhibit a bactericide and fungicide activity in vitro. the ω-aminoeth ... | 2016 | 26612063 |
| complicated intra-abdominal infections: the old antimicrobials and the new players. | complicated intra-abdominal infections (ciais) are an important cause of morbidity and mortality worldwide. they are diagnosed when the initial abdominal organ infection has spread into the peritoneal space. successful treatment relies on adequate source control and appropriate empiric antimicrobial therapy. inappropriate antimicrobial therapy may result in poor patient outcomes and increases in healthcare costs. current guidelines recommend several single and combination antimicrobial regimens; ... | 2015 | 26612473 |
| impact of colistin sulfate treatment of broilers on the presence of resistant bacteria and resistance genes in stored or composted manure. | the application of manure may result in contamination of the environment with antimicrobials, antimicrobial-resistant bacteria, resistance genes and plasmids. the aim of this study was to investigate the impact of the administration of colistin and of manure management on (i) the presence of colistin-resistant escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa and (ii) the prevalence of various antimicrobial resistance genes in feces and in composted or stored manure. one flock o ... | 2016 | 26616601 |
| characterization of a ctx-m-15 producing klebsiella pneumoniae outbreak strain assigned to a novel sequence type (1427). | extended-spectrum -lactamase producing klebsiella pneumoniae have emerged as one of the major nosocomial pathogens. between july and september 2012, a ctx-m-15 producing k. pneumoniae caused an outbreak in a university hospital in the netherlands. the outbreak isolates were characterized and assigned to a novel sequence type (st1427). an epidemiological link between affected patients was supported by patient contact tracing and whole-genome phylogenetic analysis. intra-strain polymorphism was de ... | 2015 | 26617589 |
| on-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy. | bacteriophages are bacterial viruses, capable of killing even multi-drug resistant bacterial cells. for this reason, therapeutic use of phages is considered as a possible alternative to conventional antibiotics. however, phages are very host specific in comparison to wide-spectrum antibiotics and thus preparation of phage-cocktails beforehand against pathogens can be difficult. in this study, we evaluate whether it may be possible to isolate phages on-demand from environmental reservoir. we atte ... | 2015 | 26617601 |
| prevalence of plasmid-mediated quinolone resistance genes among extended-spectrum β -lactamase-producing klebsiella pneumoniae human isolates in iran. | the purpose of this study was to determine the prevalence and molecular characterization of plasmid-mediated quinolone resistance (pmqr) genes (qnra, qnrb, qnrs, aac(6')-ib-cr, and qepa) among esbl-producing klebsiella pneumoniae isolates in kashan, iran. a total of 185 k. pneumoniae isolates were tested for quinolone resistance and esbl-producing using the disk diffusion method and double disk synergy (ddst) confirmatory test. esbl-producing strains were further evaluated for the bla ctx-m gene ... | 2015 | 26618005 |
| suppurative peritonitis by klebsiella pneumoniae in captive gold-handed tamarin (saguinus midas midas). | this report describes an outbreak of suppurative peritonitis caused by klebsiella pneumoniae in an adult female of captive golden-handed tamarin (saguinus midas midas). two virulent and multidrug-resistant strains were isolated and classified through mlst as st60 and st1263. the microbiological diagnosis works as a support tool for preventive measures. | 2016 | 26620445 |
| early rectal cancer accompanied by multiple systemic abscesses: a case report. | a 70-year-old man undergoing treatment for diabetes presented with a cough and pyrexia that has lasted one week. laboratory tests demonstrated evidence of inflammation. the patient was hospitalized and antibiotic treatment was initiated, but the condition of the patient did not improve. after 2 weeks, computerized tomography scanning demonstrated the presence of multiple small nodules in the lungs and a liver abscess. the patient also developed neck pain and numbness of the upper extremities and ... | 2015 | 26622822 |
| nosocomial emerging of (vim1) carbapenemase-producing isolates of klebsiella pneumoniae in north of iran. | the rapid emergence and dissemination of carbapenemase-producing klebsiella pneumoniae strains and other members of the enterobacteriaceae poses a considerable threat to the care of hospitalized patients and to public health. the aim of this study was to determine the frequency of metallo-β-lactamases (mbl) and vim-1 gene in multidrug-resistant strains of k. pneumoniae. | 2015 | 26622969 |
| extended-spectrum β-lactamase-producing and third-generation cephalosporin-resistant enterobacteriaceae in children: trends in the united states, 1999-2011. | enterobacteriaceae infections resistant to extended-spectrum β-lactams are an emerging problem in children. we used a large database of clinical isolates to describe the national epidemiology of extended-spectrum β-lactamase (esbl)-producing and third-generation cephalosporin-resistant (g3cr) enterobacteriaceae. | 2014 | 26625452 |
| epidemiological study of prevalence, determinants, and outcomes of infections in medical icu at a tertiary care hospital in india. | to determine the prevalence of infections, risk factors, and outcomes in a medical intensive care unit (icu), we performed a hospital-based study. | 2017 | 26628756 |
| transcriptional regulation of genes involved in 3-hydroxypropionic acid production in response to aeration of recombinant klebsiella pneumoniae. | we have described a metabolically engineered strain of klebsiella pneumoniae/puc18kan-aldhec that could convert glycerol to 3-hydroxypropionic acid (3-hp) effectively. in this study, the transcription of the genes encoding aldehyde dehydrogenase (aldh) and glycerol dehydratase (dhab) responsible to 3-hp-biosynthesis, key enzymes in pp pathway and tca cycle, as well as hydrogenases was observed under anaerobic, microaerobic, and aerobic conditions using quantitative reverse transcription-polymera ... | 2016 | 26631006 |
| synergistic interactions between doxycycline and terpenic components of essential oils encapsulated within lipid nanocapsules against gram negative bacteria. | the combination of essential oils (eos) with antibiotics provides a promising strategy towards combating resistant bacteria. we have selected a mixture of 3 major components extracted from eos: carvacrol (oregano oil), eugenol (clove oil) and cinnamaldehyde (cinnamon oil). these compounds were successfully encapsulated within lipid nanocapsules (lncs). the eos-loaded lncs were characterised by a noticeably high drug loading of 20% and a very small particle diameter of 114nm. the in vitro interac ... | 2016 | 26631640 |
| screening of nursing home residents for colonization with carbapenem-resistant enterobacteriaceae admitted to acute care hospitals: incidence and risk factors. | there are increasing reports of multidrug-resistant gram-negative bacilli in nursing homes and acute care hospitals. | 2016 | 26631643 |
| good's syndrome patients hospitalized for infections: a single-center retrospective study. | good's syndrome (gs) is a rare combination of thymoma and hypogammaglobulinemia, resulting in immunodeficiency. patients with gs are highly susceptible to bacterial infection, particularly encapsulated bacterial infection in upper and lower respiratory tracts. good's syndrome patients with moderate-to- severe infection are often hospitalized. clinical features of gs patients remain to be characterized.patients with the discharge diagnosis of gs and simultaneous infection from peking union medica ... | 2015 | 26632723 |
| epidemiology and outcome of severe sepsis and septic shock in surgical intensive care units in northern taiwan. | severe sepsis remains the leading cause of mortality in the critically ill. local epidemiological studies on sepsis are of paramount importance to increase our knowledge about sepsis features and to improve patient care and prognosis.adult patients (≥20 years) admitted to the surgical intensive care units with severe sepsis or septic shock from 2009 to 2010 were retrospectively retrieved and analyzed. the primary outcome of interest was 28-day mortality.of 7795 admissions, 536 (6.9%) patients ha ... | 2015 | 26632737 |
| notes from the field: carbapenem-resistant enterobacteriaceae producing oxa-48-like carbapenemases--united states, 2010-2015. | carbapenem-resistant enterobacteriaceae (cre) are bacteria that are often resistant to most classes of antibiotics and cause health care-associated infections with high mortality rates. among cre, strains that carry plasmid-encoded carbapenemase enzymes that inactivate carbapenem antibiotics are of greatest public health concern because of their potential for rapid global dissemination, as evidenced by the increasing distribution of cre that produce the klebsiella pneumoniae carbapenemase and th ... | 2015 | 26633574 |
| observations on the inactivation efficacy of a maldi-tof ms chemical extraction method on bacillus anthracis vegetative cells and spores. | a chemical (ethanol; formic acid; acetonitrile) protein extraction method for the preparation of bacterial samples for matrix assisted laser desorption ionisation time-of-flight mass spectrometry (maldi-tof ms) identification was evaluated for its ability to inactivate bacterial species. initial viability tests (with and without double filtration of the extract through 0.2 μm filters), indicated that the method could inactivate escherichia coli mre 162 and klebsiella pneumoniae atcc 35657, with ... | 2015 | 26633884 |
| antimicrobial, anthelmintic activities and characterisation of functional phenolic acids of achyranthes aspera linn.: a medicinal plant used for the treatment of wounds and ringworm in east africa. | achyranthes aspera linn. (amaranthaceae) commonly known as prickly chaff flower (english) is traditionally used for treating a number of ailments. different parts of the plant are used in treating wounds and ringworm in east africa and elsewhere for a number of ailments. in this study, leaf extracts of a. aspera collected from two different geographical locations (ciaat, eritrea and ukulinga, south africa) were evaluated for antibacterial, antifungal, anthelmintic activities and the plant charac ... | 2015 | 26635604 |
| genomic epidemiology of an endoscope-associated outbreak of klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae. | increased incidence of infections due to klebsiella pneumoniae carbapenemase (kpc)-producing klebsiella pneumoniae (kpc-kp) was noted among patients undergoing endoscopic retrograde cholangiopancreatography (ercp) at a single hospital. an epidemiologic investigation identified kpc-kp and non-kpc-producing, extended-spectrum β-lactamase (esbl)-producing kp in cultures from 2 endoscopes. genotyping was performed on patient and endoscope isolates to characterize the microbial genomics of the outbre ... | 2015 | 26637170 |
| klebsiella pneumoniae: characteristics of carbapenem resistance and virulence factors. | klebsiella pneumoniae, known as a major threat to public health, is the most common factor of nosocomial and community acquired infections. in this study, 50 k. pneumoniae clinical specimens isolated from bronchial, urea, blood, catheter, rectal, bile, tracheal and wound cultures were collected. these isolates were identified and carbapenem resistance was determined via an automated system, chromagar orientation and chromagar kpc. the carbapenemase gene regions (blaimp, blavim, blaoxa, blandm an ... | 2015 | 26637376 |
| analytical performance of multiplexed screening test for 10 antibiotic resistance genes from perianal swab samples. | multiantibiotic-resistant bacteria pose a threat to patients and place an economic burden on health care systems. carbapenem-resistant bacilli and extended-spectrum β-lactamase (esbl) producers drive the need to screen infected and colonized patients for patient management and infection control. | 2016 | 26637481 |
| bloodstream infections due to enterobacteriaceae among neonates in poland--molecular analysis of the isolates. | bloodstream infections (bsis) are associated with a significantly increased risk of fatality. no report has been found about the molecular epidemiology of enterobacteriaceae causing bsi in neonates in poland. the aim of this work was to determine the antibiotic resistance profiles, virulence gene prevalence, the epidemiological and genetic relationships among the isolates from enterobacteriaceae causing bsi in neonates with birth weight < 1501 g. antimicrobial susceptibility testing was performe ... | 2015 | 26638530 |
| antimicrobial use at a multi-disciplinary hospital. | the problem of antimicrobial resistance has become topical and alarming all over the world, including kazakhstan. nosocomial strains of microorganisms are widespread, being resistant to the majority of available antimicrobials. this results in longer periods of hospital stay, increases in financial expenditures, and sometimes, in lethal outcomes. the social importance of antimicrobial resistance is preconditioned by the spread of resilient strains of microorganism beyond the hospital environment ... | 2015 | 26639689 |
| regional specifics of microbial landscape in outpatients with lower respiratory tract infections. | the last years have witnessed progressive growth of antimicrobial resistance (amr) both in hospital-acquired and community-acquired respiratory infections. regional guidelines (2006) presented standard approaches to pharmacotherapy and provided an important contribution to improvement of antimicrobial therapy at healthcare facilities of both the city of stavropol and the stavropol region. at the same time, recent years have witnessed substantial changes in sensitivity to antimicrobials; besides, ... | 2015 | 26639714 |
| microbiologic analyses of patients before and after liver transplant. | the aim of this study was to analyze microbiologic screening results of patients before and after liver transplant. | 2015 | 26640931 |
| synthesis and antimicrobial evaluation of cyanopyridinyl tetrahydronaphthalene derivatives. | a novel series of cyanopyridinyl tetrahydronaphthalene incorporated with different heterocycles were synthesized. the key compounds 2a,b were condensed with chloroacetone and ethyl chloroacetate to give 3a,b and 4a,b, respectively. also condensation of 4a,b with hydrazine hydrate gave the corresponding hydrazide 5a,b. reaction of 5b with different isothiocyanates gave the corresponding thiosemicarbazide derivatives 6a-c. also, condensation of 5a with chloroacetic acid, methyl iodide and/or aceti ... | 2015 | 26642656 |
| complete sequence of blakpc-2-harboring plasmid with a mosaic of incn1- and incn3-type plasmids in a klebsiella pneumoniae isolate from south korea. | 2016 | 26643324 | |
| searching for the optimal predictor of ciprofloxacin resistance in klebsiella pneumoniae by using in vitro dynamic models. | there is growing evidence of applicability of the hypothesis of the mutant selection window (msw), i.e., the range between the mic and the mutant prevention concentration (mpc), within which the enrichment of resistant mutants is most probable. however, it is not clear if mpc-based pharmacokinetic variables are preferable to the respective mic-based variables as interstrain predictors of resistance. to examine the predictive power of the ratios of the area under the curve (auc24) to the mpc and ... | 2015 | 26643328 |
| molecular characterization by using next-generation sequencing of plasmids containing blandm-7 in enterobacteriaceae from calgary, canada. | enterobacteriaceae with blandm-7 are relatively uncommon and had previously been described in europe, india, the united states, and japan. this study describes the characteristics of enterobacteriaceae (klebsiella pneumoniae [n = 2], escherichia coli [n = 2], serratia marcescens [n = 1], and enterobacter hormaechei [n = 1] isolates) with blandm-7 obtained from 4 patients from calgary, canada, from 2013 to 2014. the 46,161-bp incx3 plasmids with blandm-7 are highly similar to other blandm-harbori ... | 2016 | 26643346 |
| molecular characterization of extended-spectrum β-lactamase-producer klebsiella pneumoniae isolates causing neonatal sepsis in peru. | klebsiella pneumoniae (kp) is the most common cause of neonatal sepsis in the low- and middle-income countries. our objective was to describe the phenotypic and molecular characteristics of extended-spectrum β-lactamase (esbl)-producer kp in neonatal care centers from peru. we collected 176 non-duplicate consecutive kp isolates from blood isolates of neonates from eight general public hospitals of lima, peru. the overall rate of esbl production was 73.3% (n = 129). the resistance rates were high ... | 2016 | 26643537 |
| nqrm (duf539) protein is required for maturation of bacterial na+-translocating nadh:quinone oxidoreductase. | na(+)-translocating nadh:quinone oxidoreductase (na(+)-nqr) catalyzes electron transfer from nadh to ubiquinone in the bacterial respiratory chain, coupled with na(+) translocation across the membrane. na(+)-nqr maturation involves covalent attachment of flavin mononucleotide (fmn) residues, catalyzed by flavin transferase encoded by the nqr-associated apbe gene. analysis of complete bacterial genomes has revealed another putative gene (duf539, here renamed nqrm) that usually follows the apbe ge ... | 2016 | 26644436 |
| chemical composition and antibacterial activity of some medicinal plants from lamiaceae family. | chemical composition and antibacterial activity of aqueous (ethanolic and methanolic) extracts from herbs often used in polish cuisine and traditional herbal medicine including thyme (thymus vulgaris l.), rosemary (rosmarinus officinalis l.), oregano (origanum vulgare l.), peppermint (mentha piperita l.) and sage (salvia officinalis l.) were compared. the aqueous ethanolic extracts contained slightly higher levels of phenolics compared to the aqueous methanolic extracts. in turn, gc-ms analysis ... | 2015 | 26647633 |
| the erratic antibiotic susceptibility patterns of bacterial pathogens causing urinary tract infections. | increasing trend of antibiotic resistance and expression of extended spectrum beta lactamases (esbls) are serious threats for public health as they render the treatment ineffective. present study was designed to elucidate the antibiotic-susceptibility patterns of esbl and non-esbl producing e. coli and k. pneumoniae causing urinary tract infections so that the ineffective antibiotics could be removed from the line of treatment. the bacterial isolates obtained from the urine of patients visiting ... | 2015 | 26648826 |
| neonatal septicemia in nepal: early-onset versus late-onset. | introduction. neonatal septicemia is defined as infection in the first 28 days of life. early-onset neonatal septicemia and late-onset neonatal septicemia are defined as illnesses appearing from birth to three days and from four to twenty-eight days postnatally, respectively. methods. in this cross-sectional study, blood samples from the suspected infants were collected and processed in the bacteriology laboratory. the growth was identified by standard microbiological protocol and the antibiotic ... | 2015 | 26649057 |
| ciprofloxacin-induced antibacterial activity is attenuated by phosphodiesterase inhibitors. | ciprofloxacin is a commonly used antibiotic for urinary tract infection that interacts with bacterial topoisomerases leading to oxidative radicals generation and bacterial cell death. phosphodiesterase inhibitors (pdeis), on the other hand, are commonly used drugs for the management of erectile dysfunction. the group includes agents such as sildenafil, vardenafil, and tadalafil. | 2015 | 26649077 |
| [rapid detection of extended-spectrum beta-lactamases by flow cytometry method]. | extended-spectrum beta-lactamases (esbl), produced by enterobacteriaceae members are enzymes that especially cause a resistance to cephalosporin group antibiotics commonly used in clinics. early and rapid detection of esbl production is crucial for antimicrobial treatment and infection control; however the methods used for this purpose are time consuming (24 to 48 hours). the aim of this study was to determine a flow cytometry based-test which provides to detect esbl producing bacteria in a shor ... | 2015 | 26649418 |
| a highly sensitive oligonucleotide hybridization assay for klebsiella pneumoniae carbapenemase with the probes on a gold nanoparticles modified glassy carbon electrode. | to develop a new electrochemical dna biosensor for determination of klebsiella pneumoniae carbapenemase, a highly sensitive and selective electrochemical biosensor for dna detection was constructed based on a glassy carbon electrode (gce) modified with gold nanoparticles (au-nano). the au-nano/gce was characterized by scanning electromicroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. the hybridization detection was measured by differential pulse voltammetry using methyle ... | 2015 | 26651586 |
| phenotypic and genotypic characterization of extended-spectrum b-lactamases producing escherichia coli and klebsiella pneumoniaein a tertiary care hospital in riyadh, saudi arabia. | extended-spectrum beta-lactamase (esbl)-producing pathogens remain a public health concern, with limited data on the molecular characterization of isolates. we aimed to determine the molecular characterization of esbl-producers circulating in our setting and correlate the molecular types with the minimal inhibitory concentration (mic) to third-generation cephalosporins. | 2015 | 26657226 |
| retrospective study on multidrug-resistant bacterium infections after rigid internal fixation of mandibular fracture. | to retrospectively investigate infection by multidrug-resistant bacteria (mdrb) after rigid internal fixation (rif) of mandibular fracture and determine risk factors and cure methods. | 2016 | 26657396 |
| mortality due to blakpc klebsiella pneumoniae bacteraemia. | to determine the mortality rate secondary to blakpc klebsiella pneumoniae (kpc/kp) bacteraemia, compared with that from esbl-producing k. pneumoniae (esbl/kp) bacteraemia, and to determine associated risk factors. | 2016 | 26661396 |
| prediction of type ii toxin-antitoxin loci in klebsiella pneumoniae genome sequences. | klebsiella pneumoniae is an increasingly important bacterial pathogen to human. this gram-negative bacterium species has become a serious concern due to its dramatic increase in the levels of multiple antibiotic resistances, particularly to carbapenems. the toxin-antitoxin (ta) system has recently been reported to be involved in the formation of drug-tolerant persister cells. the type ii ta system is composed of a stable toxin protein and a relatively unstable antitoxin protein that is able to i ... | 2016 | 26662948 |
| septic knee arthritis in crohn's disease biological therapy-free patient. case report. | a 52-year-old woman with crohn's disease presented with septic arthrtis of the knee. this condition coincided with a symptomatic flare of her crohn's disease due to an ileal inflammatory stenosis, manifested as a phlegmonous mass palpable in the right lower quadrant and a small bowel obstruction. results of synovial fluid cultures showed the presence of gram-negative bacillus, klebsiella pneumoniae and the ct scan images were highly suggestive of abdominal abscess within crohn's disease. the pat ... | 2017 | 26664477 |
| [optimization of labeling and localizing bacterial membrane and nucleus with fm4-64 and hoechst dyes]. | to observe cell membrane and nucleus in bacteria for subcellular localization. | 2015 | 26665605 |
| correlation of β-lactamase production and colistin resistance among enterobacteriaceae isolates from a global surveillance program. | the increasing use of carbapenems for treating multidrug-resistant (mdr) gram-negative bacterial infections has contributed to the global dissemination of carbapenem-resistant enterobacteriaceae (cre). serine and metallo-β-lactamases (mbls) that hydrolyze carbapenems have become prevalent and endemic in some countries, necessitating the use of older classes of agents, such as colistin. a total of 19,719 isolates of enterobacteriaceae (excluding proteeae and serratia spp., which have innate resis ... | 2016 | 26666920 |
| emergence of oxa-162 carbapenemase- and dha-1 ampc cephalosporinase-producing sequence type 11 klebsiella pneumoniae causing community-onset infection in greece. | oxa-48-like carbapenemases have only recently emerged in europe. oxa-162 is a rare oxa-48 variant usually coexpressed with extended-spectrum β-lactamases. here, we report the identification of the first oxa-162 carbapenemase-producing klebsiella pneumoniae isolates, which coexpressed an ampc cephalosporinase (dha-1), retrieved from a patient in greece. they belonged to a single sequence type (st11) and caused the first documented community-onset urinary tract infections attributable to an oxa-48 ... | 2016 | 26666930 |
| porin loss impacts the host inflammatory response to outer membrane vesicles of klebsiella pneumoniae. | antibiotic-resistant strains of klebsiella pneumoniae often exhibit porin loss. in this study, we investigated how porin loss impacted the composition of secreted outer membrane vesicles as well as their ability to trigger proinflammatory cytokine secretion by macrophages. we hypothesize that porin loss associated with antibiotic resistance will directly impact both the composition of outer membrane vesicles and their interactions with phagocytic cells. using clonally related clinical isolates o ... | 2015 | 26666932 |
| role of the outer membrane and porins in susceptibility of β-lactamase-producing enterobacteriaceae to ceftazidime-avibactam. | this study examined the activity of the novel antimicrobial combination ceftazidime-avibactam against enterobacteriaceae exhibiting different outer membrane permeability profiles, specifically with or without porins and with or without expression of the main efflux pump (acrab-tolc). the addition of the outer membrane permeabilizer polymyxin b nonapeptide increased the antibacterial activities of avibactam alone, ceftazidime alone, and ceftazidime-avibactam against the characterized clinical iso ... | 2015 | 26666933 |
| complete sequence of a novel incr-f33:a-:b- plasmid, pkp1034, harboring fosa3, blakpc-2, blactx-m-65, blashv-12, and rmtb from an epidemic klebsiella pneumoniae sequence type 11 strain in china. | a high fosfomycin resistance rate was observed in klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae (kpc-kp) in our previous study, but little is known about its mechanisms. in this study, we explored the prevalence of plasmid-mediated fosfomycin resistance determinants among fosfomycin-resistant kpc-kp strains from a chinese university hospital and determined the complete sequence of a novel fosa3-carrying plasmid isolated from an epidemic k. pneumoniae sequence type (st) 11 str ... | 2016 | 26666939 |
| antimicrobial susceptibility trends among gram-positive and -negative clinical isolates collected between 2005 and 2012 in mexico: results from the tigecycline evaluation and surveillance trial. | the tigecycline evaluation and surveillance trial (t.e.s.t) is a global antimicrobial surveillance study of both gram-positive and gram-negative organisms. this report presents data on antimicrobial susceptibility among organisms collected in mexico between 2005 and 2012 as part of t.e.s.t., and compares rates between 2005-2007 and 2008-2012. | 2015 | 26667651 |
| polyphenolic secondary metabolites synergize the activity of commercial antibiotics against clinical isolates of β-lactamase-producing klebsiella pneumoniae. | emergence of worldwide antimicrobial resistance prompted us to study the resistance modifying potential of plant-derived dietary polyphenols, mainly caffeic acid, ellagic acid, epigallocatechin-3-gallate (egcg) and quercetin. these compounds were studied in logical combination with clinically significant antibiotics (ciprofloxacin/gentamicin/tetracycline) against klebsiella pneumoniae, after conducting phenotypic screening of a large number of clinical isolates and selecting the relevant strains ... | 2016 | 26668123 |
| assessment of microbial load of un-pasteurized fruit juices and in vitro antibacterial potential of honey against bacterial isolates. | the development of resistance in bacteria against commonly used antibiotics/drugs is of considerable medical significance. aim of this study was to determine the microbial load of un-pasteurized packed fruit juices sold in lahore city and to determine antibacterial activity of five different honey samples against isolated bacteria. unpasteurized fruit juice samples (n=60) were collected from street vendors. all the samples were subjected to total viable count (tvc), staphylococcal count (sc) and ... | 2015 | 26668658 |
| extended-spectrum β-lactamase and carbapenemase production among burn and non-burn clinical isolates of klebsiella pneumoniae. | klebsiella pneumoniae is an opportunistic pathogen responsible for up to 10% of nosocomial infections. the emergence and spread of multidrug resistant k. pneumoniae, mostly due to the production of extended-spectrum β-lactamases (esbl) and carbapenemases, is often responsible for antibiotic treatment failure of these infections. we compared the antibiotic resistance profiles, esbl and carbapenemase production as well as presence of kpc-type genes in burn and non-burn clinical isolates of k. pneu ... | 2015 | 26668701 |
| [study of rapid species identification of bacteria in water]. | multi-wavelength ultraviolet visible (uv-vis) transmission spectra of bacteria combined the forward scattering and absorption properties of microbes, contains substantial information on size, shape, and the other chemical, physiological character of bacterial cells, has the bacterial species specificity, which can be applied to rapid species identification of bacterial microbes. four different kinds of bacteria including escherichia coli, staphylococcus aureus, salmonella typhimurium and klebsie ... | 2015 | 26669181 |
| the spread of ctx-m-type extended-spectrum β-lactamases in brazil: a systematic review. | the aim of this study was evaluate the spread of ctx-m-type extended-spectrum β-lactamase variants in microorganisms involved in both hospital- and community-acquired infections in different regions of brazil to determine their epidemiology and identify areas for further research. thirty-six studies were included and analyzed. most of the studies were conducted in the southeastern (66.7%, 24/36), southern (22.2%, 8/36), and northeastern (8.3%, 3/36) regions of brazil. no study was conducted in t ... | 2016 | 26669767 |
| prostate lymphoma complicated with prostatorectal fistula and multifocal infection after chemotherapy. | a 67-year-old man with a biopsy proving prostate diffuse large b-cell lymphoma was referred for an f-fdg pet/ct scan for postchemotherapy treatment monitoring. the fdg pet/ct scan revealed a significant reduction of prostate lymphoma and a prostatorectal fistula formation. the patient developed klebsiella pneumoniae sepsis, and a follow-up fdg pet/ct 3 months later revealed multifocal lung, bone, and muscle infection. the fdg pet/ct scans of this patient not only demonstrated the effectiveness o ... | 2016 | 26673235 |
| [antimicrobial resistance monitoring of gram-negative bacilli isolated from 15 teaching hospitals in 2014 in china]. | to investigate the current situation of antimicrobial resistance of nosocomial gram-negative bacilli in 2014 in china. | 2015 | 26675021 |
| deep sequencing-based analysis of gene expression in bovine mammary epithelial cells after staphylococcus aureus, escherichia coli, and klebsiella pneumoniae infection. | the goal of this study was to characterize the transcriptome of primary bovine mammalian epithelial cells (pbmecs) and to identify candidate genes for response and resistance to staphylococcus aureus (strain s108), escherichia coli (strain e23), and klebsiella pneumoniae (strain k96) infection. using solexa sequencing, approximately 4.9 million total sequence tags were obtained from each of the three infected libraries and the control library. gene ontology (go) analysis of the s108-infected pbm ... | 2015 | 26681042 |
| intrathecal administration of colistin for meningitis due to new delhi metallo-β-lactamase 1(ndm-1)-producing klebsiella pneumoniae. | infection by bacteria carrying new delhi metallo-β-lactamase 1 (ndm-1) is becoming a global health problem. we report a case of meningitis caused by ndm-1-producing klebsiella pneumoniae, for which intrathecal administration of colistin was curative. a previously healthy 38-year-old japanese man, who lived in hyderabad, india, suddenly collapsed and was brought to a local hospital. he was diagnosed with subarachnoid hemorrhage and underwent emergency surgery which included partial skull removal. ... | 2016 | 26683242 |
| hospital readmissions in patients with carbapenem-resistant klebsiella pneumoniae. | various transmission routes contribute to spread of carbapenem-resistant klebsiella pneumoniae (crkp) in hospitalized patients. patients with readmissions during which crkp is again isolated ("crkp readmission") potentially contribute to transmission of crkp. | 2016 | 26686227 |
| carbapenem-resistant klebsiella pneumoniae isolates from egypt containing blandm-1 on incr plasmids and its association with rmtf. | the aim of this study was to characterize carbapenem-resistant klebsiella pneumoniae (crkp) isolates recovered from clinical specimens at a tertiary care hospital in egypt over a period of 15 months. | 2016 | 26686939 |
| deep neck infections of odontogenic origin and their clinical significance. a retrospective study from hradec králové, czech republic. | cellulitis remains a very serious disease even today. mortality, which varied between 10-40%, has been reduced owing to the standard securing of airway patency and use of an appropriate surgical treatment approach. | 2015 | 26686948 |