| hepatic abscess and osteomyelitis from klebsiella pneumoniae. | | 1978 | 361992 |
| polarity of mutations induced by insertion of transposons tn5, tn7 and tn10 into the nif gene cluster of klebsiella pneumoniae. | three new genes nifm, nifi and nifn have been mapped in the nif gene cluster of klebsiella pneumoniae and a fourth gene nifj has been confirmed as being a separate cistron. polar nif mutations were obtained by transposition of tn7 to plasmid prd1, and of tn5 and tn10 to plasmid pmf100, a derivative of prd1. complementation analysis of the nif::tn mutants led to the identification of at least six transcriptional units: nifb; nifa; nifj; nifh, nifd and nifk; nife and nifi; nifn, nifm and niff. bio ... | 1978 | 362160 |
| antiserum treatment of gram-negative bacteremia. | in order to lower the mortality rate from gram-negative bacteremia, 136 patients were treated with human antiserum against core glycolipid, or with control nonimmune human serum, in a double-blind clinical trial. the antiserum was prepared by immunizing healthy young men with a vaccine composed of heat killed cells of the j5 mutant of e. coli 0111 b4. since the core glycolipid in this mutant is not encumbered with "o" side chains, it can stimulate antibody against the core glycolipid possessed i ... | 1978 | 362528 |
| bladder surface mucin. its antibacterial effect against various bacterial species. | we previously reported the results of quantitative and histochemical studies implicating the surface mucin of the bladder mucosa as an important antibacterial defense mechanism, which functions by preventing bacteria from adhering to the bladder wall. we call the mucin "anti-adherence factor" and we feel this is a previously undocumented role for mucin as a type of host antibacterial defense. these experiments were conduced with escherichia coli. in an effort to determine whether the anti-adhere ... | 1978 | 362941 |
| pharyngeal flora in ambulatory alcoholic patients: prevalence of gram-negative bacilli. | the pharyngeal flora of a group of ambulatory alcoholic patients was studied and compared with the pharyngeal flora of a control group. sixty-eight patients were studied, 34 alcoholics and 28 controls. of the alcoholic patients, 59% had gram-negative bacilli in their pharyngeal flora, while 14% of the control group had the same organisms. there were no differences in gram-positive cocci colonization between the groups. klebsiella pneumoniae was the most frequent isolate (40%) and the klebsiella- ... | 1978 | 363086 |
| nitrogenase of klebsiella pneumoniae: a pre-steady state burst of atp hydrolysis is coupled to electron transfer between the component proteins. | | 1978 | 363454 |
| intraventricular administration of amikacin for complicated gram-negative meningitis and ventriculitis. | four pediatric neurosurgical patients with gram-negative meningitis and ventriculitis were treated with parenteral and intraventricular amikacin, a new aminoglycoside. the organisms infecting these patients were resistant to multiple antimicrobial drugs but were sensitive to amikacin. treatment was continued for 14 days after cerebrospinal fluid cultures became negative. all four patients were cured and have demonstrated no nephrotoxicity, ototoxicity, or evidence of persistent infection on foll ... | 1979 | 363982 |
| effect of cardiopulmonary bypass on in vivo clearance of live klebsiella aerogens. | | 1977 | 364718 |
| pathophysiologic responses to klebsiella aerogenes bacteria: 24-hour observations. | | 1977 | 364736 |
| acquisition of multiple antibiotic resistance by salmonella dublin from the gramnegative hospital flora, in a kidney allograft recipient. | the case of a kidney allograft recipient, who suffered from several episodes of salmonella dublin sepsis following massive immunosuppressive therapy to overcome a transplant rejection crisis, is presented. the focus of sepsis was the chronic inflamed gallbladder. the salmonella dublin strain isolated from the blood during the last episode was found to exhibit multiple resistance to antimicrobiol drugs. because the resistance phenotype was characteristic for the gramnegative flora of the universi ... | 1978 | 364885 |
| synergistic activity of gentamicin with trimethoprim or sulfamethoxazole-trimethoprim against escherichia coli and klebsiella pneumoniae. | the effect of combinations of gentamicin with trimethoprim or sulfamethoxazole-trimethoprim against clinical isolates of escherichia coli (11 strains) and klebsiella pneumoniae (12 strains) was examined by using a microdilution checkerboard technique. all isolates were susceptible to each antimicrobial agent. synergism, defined as at least a 2-log(2)-dilution lowering of the minimal inhibitory concentration of either antibiotic in the combination compared with the minimal inhibitory concentratio ... | 1978 | 365085 |
| primary klebsiella identification with macconkey-inositol-carbenicillin agar. | macconkey-inositol-carbenicillin agar has successfully been used as a primary selective medium for klebsiella enumeration. with pure cultures, nearly 100% recovery of klebsiella was observed by membrane filtration. with environmental samples using membrane filtration, 95% of typical pink- to red-colored colonies were verified as klebsiella, as opposed to only 1% of yellow background colonies. recovery of klebsiella on macconkey-inositol-carbenicillin agar was as good or better than on mendo agar ... | 1978 | 365108 |
| technical aspects of separation using aqueous two-phase systems in enzyme isolation processes. | | 1978 | 365256 |
| procedure for the simultaneous large-scale isolation of pullulanase and 1,4-alpha-glucan phosphorylase from klebsiella pneumoniae involving liquid-liquid separations. | a procedure for the simultaneous large-scale isolation of pullulanase and 1,4-alpha-glucan phosphorylase from klebsiella pneumoniae is described. the pullulanase is solubilized from the cell wall by cholate treatment; cells and cell debris are removed by partition in a poly(ethylene glycol) (peg)-dextran two-phase system and from the upper (peg) phase of this system the pullulanase is isolated by ultrafiltration and precipitation with n-cetyl,n-,n-,n-trimethyl ammonium bromide to a purity of abo ... | 1978 | 365257 |
| effect of monocontamination of germfree rats by enterotoxigenic coliform bacteria. | | 1979 | 365667 |
| antibody-independent interaction of the first component of complement with gram-negative bacteria. | the interaction of the first component of complement with two serum-sensitive strains of escherichia coli and klebsiella pneumoniae was studied. it could be demonstrated that highly purified c1, free of immunoglobulin g and immunoglobulin m, binds to e. coli or k. pneumoniae. c1 binding was also found with specifically absorbed human serum, after incubation of bacteria with normal serum in the presence of ethylenediaminetetraacetate or agammaglobulinemic serum; the number of c1 molecules taken u ... | 1978 | 365750 |
| the capsules of corynebacterium equi and streptococcus equi. | the capsules of corynebacterium equi and streptococcus equi were examined by electron microscopy after staining with ruthenium red. they were compared with the capsule of klebsiella pneumoniae which had previously been examined using the same procedure (springer & roth, 1973). the capsule of c. equi had a laminated appearance. when s. equi was grown on solid medium, its capsule appeared as radially arranged projections capped by a thick electron dense layer. when grown in liquid medium, s. equi ... | 1978 | 366078 |
| modulation of influenza in mice by transfer factor therapy. | | 1978 | 366134 |
| close association between shape alteration and loss of immunity to superinfection in a wild-type klebsiella pneumoniae stable lysogen which can be both immune and nonimmune to superinfection. | klebsiella pneumoniae mirm7 is a wild-type strain which grows as cocci at ph 7 and above and as rods at ph 6.5 and below. cultures of this strain and an auxotrophic derivative, mirm7b, have been found to undergo spontaneous lysis after purification from possible contaminating viruses. lysates always contained two phages, fr2 and ap3, most often at high titers. fr2 and ap3 plated with the same efficiency on both mirm7b and k59 (another k. pneumoniae strain sensitive to fr2 and ap3) and lysogenize ... | 1978 | 366180 |
| lysogenic conversion in klebsiella pneumoniae: system which requires active immunity regulation for expression of the conversion phenomenon. | we have previously described klebsiella pneumoniae mirm7b, which, although stably lysogenic for the inducible and nondefective phages fr2 and ap3, is not immune to superinfection by these same viruses. mira12b, a strain which is lysogenic for fr2 and ap3 and immune to superinfection, has been derived from mirm7b. the sensitivity of this strain and that of the nonimmune parent to several bacteriophages have been compared in this work. it has been found that, whereas mirm7b is sensitive to colipha ... | 1978 | 366181 |
| [synergistic effect of ampicillin and dicloxacillin on penicillin and cephalosporin-susceptible escherichia coli (author's transl)]. | an effect of a combination of ampicillin (abpc) and dicloxacillin (mdipc) on penicillins and cephalosporin-susceptible e. coli was determined. minimum inhibitory concentrations of the combined drug were as same as that of abpc, but bacterial growth was inhibited by the combined drug stronger than abpc, low activity of beta-lactamase was detected in sonicated e. coli 0.225 cells, and this activity was inhibited by relatively low concentration of mdipc. we considered that a weak synergism of the c ... | 1978 | 366193 |
| genetic and biochemical analysis of mutants induced by bacteriophage mu dna integration into klebsiella pneumoniae nitrogen fixation genes. | polar mutations were obtained by integration of bacteriophage mu c+ or mu cts dna into the klebsiella pneumoniae nif genes located on plasmid pce1, a derivative of prd1. in addition, nif deletions were isolated from nif::mu cts plasmids. complementation data allowed the characterization of twelve nif cistrons, nine corresponding to previously identified genes. polar effect of mu dna insertions suggested the existence of at least six transcription units: 1) nif k, nif d and nif h--2)nif a and nif ... | 1978 | 366377 |
| klebsiella pneumoniae and acute anterior uveitis in ankylosing spondylitis. | | 1979 | 367506 |
| klebsiella pneumoniae infection with secondary hypopyon in tokay gecko lizards. | | 1978 | 367993 |
| the configuration and location of the ribosidic linkage in the prosthetic group of citrate lyase (klebsiella aerogenes). | the structure of the prosthetic group of citrate lyase (klebsiella aerogenes) was studied by nuclear magnetic resonance and mass spectrometry. the spectra at 360 mhz of the nucleoside moiety (2'-ribosyladenosine) show the absence of 2'-hydroxyl proton, thus confirming the 2' position as the site of attachment of the second ribose moiety to the dephospho-coa. this glycosidic linkage is found to be alpha(1" leads to 2') and is identical to that of poly(adp-ribose). studies of permethylation produc ... | 1979 | 368065 |
| chemotherapy of pneumonias. | | 1979 | 368330 |
| microbial adjuvant and autoimmunity. iii. histological studies of development of experimental autoimmune thyroiditis in mice immunized with syngeneic thyroid extract together with the capsular polysaccharide of klebsiella pneumoniae. | | 1978 | 368527 |
| [studies on the bacterial causes of neonatal mortality in foals. report on post-mortem findings (author's transl)]. | the causes of neonatal mortality in foals were studied over a period of two years. the total number of foals studied was 121. bacterial infection was found to be an important factor. infection caused by a. equuli (1.6%) which previously was the most important one, has been superseded by e. coli infection (56%). e. coli infections particularly occur during the first weeks of life and, depending on the course of the disease, give rise to various pathological changes. infections running an acute co ... | 1979 | 369036 |
| dental infection in a cirrhotic patient. source of recurrent sepsis. | a patient with alcoholic cirrhosis had multiple episodes of sepsis with klebsiella pneumonia. repeated searches for the source of infection finally revealed the organism in the root of a tooth. evidence indicated that this site was the primary source of infection. the importance of dental infections in alcoholics and the difficulty in diagnosing those infections are emphasized by this case. | 1979 | 369935 |
| transfer by bone marrow cells of increased natural resistance to klebsiella pneumoniae induced by lipopolysaccharide in genetically deficient c3h/he mice. | in a previous study we demonstrated that lipopolysaccharide failed to elicit nonspecific resistance in c3h/he lipopolysaccharide low-responder mice against klebsiella infection in contrast to its activity in a closely related histocompatible high-responder subline, c3heb/fe. complete restoration of lipopolysaccharide-induced protection against 10(5) klebsiella was obtained in the present study by transferring bone marrow from high-responder mice to the highly deficient c3h/he mice. the ability o ... | 1979 | 370012 |
| enzyme-linked immunospecific antibody test for detecting antibody to klebsiella. | the enzyme-linked immunospecific antibody test was performed in standard test tubes and microtiter plates to meausre high-titer antibody against klebsiella capsular polysaccharide. initial studies were conducted with rabbit sera; other studies were conducted with the serum of a patient infected with type 9 klebsiella. both immunized rabbits and an infected patient disclosed high titers of anticapsular antibody. control sera from other immunized rabbits and other infected humans failed to show t ... | 1978 | 370145 |
| simultaneous nosocomial outbreaks caused by multiply resistant klebsiella pneumoniae types 2 and 30. | two simultaneous klebsiella pneumoniae outbreaks resistant to multiple antibiotics are reported. the closely related strains were distinguished by comparing the zone diameters obtained by disk diffusion susceptibility testing but not by interpretations of susceptible, resistant, or intermediate. similar conclusions were made when minimal inhibitory concentrations were considered. the separation was subsequently confirmed by capsular serotyping into types 2 and 30. the data presented suggest that ... | 1978 | 370152 |
| thermosensitive h1 plasmids determining citrate utilization. | twelve thermosensitive h1 plasmids from strains of salmonella typhi that had caused outbreaks of chloramphenicol-resistant typhoid fever in vietnam, thailand and india mediated citrate utilization (cit+) in a prototrophic escherichia coli k12 strain but not in the s. typhi strains from which they were derived. four h1 plasmids from a similar outbreak in mexico differed from the far eastern plasmids in not mediating citrate utlization but in mediating mercury resistance. h1 plasmids resembling th ... | 1978 | 370344 |
| epidemiologic and bacteriologic evaluation of neonatal necrotizing enterocolitis. | the incidence of necrotizing enterocolitis (nec) in our neonatal unit has varied from 4.7% to zero to 4.4% during three time periods. simultaneously, significant changes have occurred in the spectrum of bacterial species in the gastrointestinal tract of unaffected infants in the same unit. during the first period of increased attack rate, 82% of gastric and 88% of fecal enterobacteriaceae were e. coli and k. pneumoniae. when the attack rate decreased the frequencies were 11% (gastric) and 47% (f ... | 1979 | 370356 |
| bacteriocin typing of serratia marcescens. a simplified system. | the authors describe a simplified system for the detection of bacteriocin production by serratia marcescens with the use of six indicator strains, which include escherichia coli, klebsiella pneumoniae, citrobacter diversus, enterobacter aerogenes (two strains), and serratia rubidaea grown on arabinose minimal medium plates. of the 64 possible bacteriocin types, 11 were observed; 66% of the isolates tested were found to be one of three types. occasionally more than one bacteriocin type was observ ... | 1979 | 371382 |
| endotoxin in germfree, gnotobiotic, or conventional-flora sprague-dawley rats. | the limulus assay for bacterial endotoxin was performed on serum and (or) plasma from animals monoassociated with clostridium species, staphylococcus aureus, escherichia coli, proteus mirabilis, enterobacter agglomerans, bacteroides fragilis, klebsiella pneumoniae, or candida albicans. plasma from animals monoassociated with the gram-negative bacteria or c. albicans consistently showed a positive limulus test while conventional-flora controls, germfree rats, and gnotobiotic animals monoassociate ... | 1978 | 371771 |
| synthesis and excretion of polygalacturonic acid trans-eliminase in erwinia, yersinia, and klebsiella species. | | 1979 | 371775 |
| klebocin typing of klebsiella pneumoniae. | | 1979 | 372098 |
| trimethoprim in combination with a sulfonamide for oral treatment of canine urinary tract infections. | trimethoprim, in combination with sulfadiazine or sulfamethoxazole was administered orally for 7 to 14 days to 84 dogs with urinary tract infections (uti). the daily dosage of 26.4 mg/kg (12 mg/lb) was divided into 2 equal parts and administered at about 12-hour intervals. response to treatment, based on negative urine culture during or after therapy, was 37 of 45 (82%) for uti caused by escherichia coli, 11 of 15 (73%) uti caused by proteus mirabilis, 8 of 12 (67%) uti caused by klebsiella pneu ... | 1979 | 372165 |
| cefuroxime therapy for urinary tract infections caused by a multi-resistant, epidemic klebsiella aerogenes. | | 1979 | 372170 |
| sensitivity, precision, and accuracy of the y1 adrenal cell enterotoxin assay. | the y1 adrenal cell assay for heat-labile enterotoxin (lt) was found to be relatively insensitive for filtrates of toxigenic escherichia coli h10407. when observations were made blindly and subjected to rigorous controls, reliable detection of lt occured only at filtrate dilutions from 1:4 to 1:10. detection of lt was unreliable when e. coli h10407 was mixed with another enteric organism. overall, 7.1% of observers' reports from a single assay were imprecise, and 2.6% would have resulted in erro ... | 1979 | 372224 |
| reproducibility of an indirect immunofluorescent-antibody technique for capsular serotyping of klebsiella pneumoniae. | reproducibility of capsular serotypes of 55 consecutive clinical isolates of klebsiella pneumoniae was evaluated by an indirect immunofluorescent-antibody technique previously described by riser et al. (j. clin. pathol. 29: 296-304, 1976) five colonies per specimen were examined for colony-to-colony variation, day-to-day variation, and reader-to-reader variation. seventy-two reference strains were tested with each of 18 pools and 72 specific antisera prior to the clinical specimens to determine ... | 1979 | 372225 |
| the development and assessment of a bacteriocin typing method for klebsiella. | klebsiellas are generally typed by the method of capsular serotyping but, although this is a reliable method, it is time consuming, requires the production of a large number of antisera and is not generally available. for this reason another method for typing klebsiellas was sought. a bacteriocin typing method involving mitomycin c induction was developed and the cultural conditions giving optimum klebecin production and the best methods of testing the sensitivity of the organisms to klebecins w ... | 1979 | 372440 |
| [a case of skeletal dissemination of septic foci with disastrous outcome (proceedings)]. | | 1978 | 373023 |
| [phage typing of rough e. coli strains isolated from urine (author's transl)]. | a differentiation of e. coli rough strains is generally not well established in bacteriological urine diagnosis although these strains are relatively often isolated from urine samples of patients with urinary tract infections (see table 2). an exact characterization of rough strains seems to be necessary especially for the distinction between recurrent and reinfection during therapy and follow-up studies. the application of phage typing for characterization of e. coli rough strains isolated from ... | 1978 | 373321 |
| lymph follicular cholecystitis. | ten cases of cholecystitis with severe lymphocytic reactions were selected among serially examined 131 cases of cholecystitis or/and cholelithiasis. gram-negative bacterial infection, especially e. coli and k. pneumonia seemed to be related as the cause of these severe lymphocytic reactions, but the gallstone revealed no definite influence. we would like to separate the cases with severe lymphocytic reactions as a lymph follicular cholecystitis and consider the possibility of a gram-negative bac ... | 1979 | 373378 |
| histamine production by klebsiella pneumoniae and an incident of scombroid fish poisoning. | a histamine-producing strain of klebsiella pneumoniae was isolated from a sample of tuna sashimi implicated in an outbreak of scombroid fish poisoning. none of the other nine gram-negative bacterial strains isolated from the tuna sashimi was capable of equivalent histamine production. bacterial histamine production was monitored in a tuna fish infusion broth (tfib), and the implicated k. pneumoniae was capable of producing 442 mg of histamine per 100 g of tuna in tfib in 7 h under controlled inc ... | 1979 | 373626 |
| klebsiella pneumoniae. | | 1978 | 373650 |
| overflow metabolism in aerobic micro-organisms. | | 1979 | 374156 |
| [treatment of psoriasis using vitamin a, vitamin a acid and oral retinoids]. | vitamin a, vitamin a acid (retinoic acid) and their synthetic derivatives were variously applied in the management of psoriasis with different therapeutic results. obviously, they influence the proliferation rate and the differentiation of human keratinizing epithelia and have beneficial effects on skin diseases with disturbances of keratinization, including psoriasis. systemic application of vitamin a has been yet largely abandonned since high dosages leading to clearing develop evident systemi ... | 1979 | 374312 |
| association of tellurium resistance and bacteriophage inhibition conferred by r plasmids. | concomitant resistance to tellurium compounds (ter) and inhibition of coli-phage development (phi) are properties mediated by many h2 incompatibility group r plasmids which have been isolated from diverse bacterial and geographic sources. ter plasmids from tellurium-resistant bacteria that were isolated from sewage and industrial wastes also mediated phage inhibition. of these ter plasmids, three from citrobacter freundii belonged to the h incompatibility group, whereas three from klebsiella pne ... | 1979 | 374351 |
| antimicrobial therapy of septicemia due to klebsiella pneumoniae in neutropenic rats. | three different isolates of klebsiella pneumoniae, highly sensitive to amikacin but varying in susceptibility to cefazolin, were injected intraperitoneally into neutropenic rats. animals were treated every 8 hr for 72 hr with saline (controls), cefzolin (full dose, 40 mg/kg; one-fourth dose, 10 mg/kg), amikacin (full dose, 8 mg/kg; one-fourth dose, 2 mg/kg), or a combination of both drugs at either full dose or one-fourth dose. all drugs were given intramuscularly. combination therapy with full ... | 1979 | 374646 |
| spontaneous degradation of prd1 dna into unique size classes is reca dependent. | the his and nif genes of the p1 type plasmid prd1 were lost at a high frequency in a reca+ but not in a reca- escherichia coli host during growth in a non-selective medium. 92% of the his- nif- segregants after 6 subcultures retained the genetic markers of the precursor plasmid rp4, while the remainder lost all of the prd1 markers with the concomitant loss of ccc-dna. plasmids purified from the his- nif- segregants resembled rp4 in the physical and genetic properties examined. the contour length ... | 1979 | 375020 |
| the mutagenic action of nitroimidazoles. iv. a comparison of the mutagenic action of several nitroimidazoles and some imidazoles. | the mutagenic action of 51 imidazoles was investigated. the fluctuation test of luria and delbrück was used, with klebsiella pneumoniae as test organism. 8 compounds, including 5 with a weak mutagenic action in the fluctuation test, were also investigated by the ames test in which salmonella typhimurium ta100 was used. of the 51 imidazoles examined, 33 were nitroimidazoles. 31 of the latter appeared to be mutagenic, whereas out of the 18 other imidazoles without a nitro group only 2 were mutagen ... | 1979 | 375080 |
| protein-rna crosslinks in ribosomes: ultraviolet action spectra. comparison with inactivation action spectrum. | | 1979 | 375249 |
| dna turnover in buffer-held escherichia coli and its effect on repair of uv damage. | | 1979 | 375250 |
| enzymatic activities in ultraviolet-induced dna degradation in escherichia coli. | | 1979 | 375280 |
| prostaglandins, their intermediates and precursors: cardiovascular actions and regulatory roles in normal and abnormal circulatory systems. | the characterization of newly found unstable metabolites of arachidonic acid has provided new perspectives for cardiovascular regulatory mechanisms and new insights into disorders of the circulatory system. since these intermediates are often more potent on and more specific for cardiovascular structures than the classical prostaglandins, they are more likely candidates as physiologic mediators of circulatory events. their instability in vitro need not preclude these roles; on the contrary, the ... | 1979 | 375296 |
| [biosynthesis and assembly of outer membrane proteins of escherichia coli (author's transl)]. | | 1979 | 375315 |
| [sepsis caused by candida albicans. comments on 7 cases]. | | 1979 | 375329 |
| gram-negative bacillary sepsis associated with use of lipid emulsion in parenteral nutrition. | | 1979 | 375720 |
| coenzyme b12-dependent diol dehydrase: purification, subunit heterogeneity, and reversible association. | | 1979 | 375836 |
| nosocomial klebsiella pneumoniae epidemic in a neonatal special care unit. | | 1978 | 376441 |
| nursing care study. patient on a ventilator: beyond the speech barrier. | | 1979 | 377248 |
| a comparison of the antibacterial activity of sulphonamide-trimethoprim combinations. | sensitivity patterns of 326 clinical isolates toward sulphonamide/trimethoprim combinations were tested in vitro. the relative merits of the different combinations are discussed in their pharmacological and bacteriological contexts. it is concluded that organisms sensitive to cotrimoxazole as judged by in vitro sensitivity tests performed at 37 degrees c are also sensitive to sulphamoxole/trimethoprim. | 1979 | 377523 |
| characterization of motile and acetoin-negative klebsiella pneumoniae strains by dna: dna by hybridization. | hybridization studies were carried out to determine the extent of divergence between different members of the enterobacteriaceae and group d, previously described by numerical analysis of phenotypic characters, and comprising strains isolated from surface waters. this group, apparently belonging in or related to the genus citrobacter on the basis of the imvic tests differs from this taxon by numerical analysis and dna base composition. our work reveals a dna relatedness of 81-89% between the cen ... | 1978 | 378119 |
| respiratory nitrate reductase: its localization in the cytoplasmic membrane of klebsiella aerogenes and bacillus licheniformis. | the sidedness of the respiratory nitrate reductase in the cytoplasmic membrane of bacillus licheniformis and klebsiella aerogenes was studied by indirect immunofluorescence and by lactoperoxidase-catalyzed iodination. it was shown that the two subunits (mr 150000 and 57000, respectively) of nitrate reductase of b. licheniformis are localized on the cytoplasmic side of the membrane, whereas the k. aerogenes enzyme is a transmembrane protein. the different localization of nitrate reductase in the ... | 1979 | 378657 |
| capsular serotypic specificity of the protection conferred on mice by klebsiella pneumoniae ribosomal preparations. | klebsiella pneumoniae ribosomal preparations protect mice immunized by the subcutaneous route against an intraperitoneal challenge of 100 50% lethal doses. the minimal protective doses are 5 and 0.4 micrograms of proteins for preparations extracted from strains of capsular serotypes 1 and 2, respectively. this difference in protective activity is also found in bacteria killed by formalin. the protective activity of these preparations is not diminished by their purification on sucrose gradient, w ... | 1979 | 378855 |
| order of genes near nif in klebsiella pneumoniae. | analysis of strains with deletions of all or part of nif have ordered the klebsiella pneumoniae genetic loci as thi rbt dal udk gnd rfb has nif shia. the his-nif plasmids prd1 and ptm4010 contain the genes gnd rfb his nif shia. | 1979 | 378930 |
| fermentation of 1,2-propanediol with 1,2-ethanediol by some genera of enterobacteriaceae, involving coenzyme b12-dependent diol dehydratase. | klebsiella pneumoniae (aerobacter aerogenes) atcc 8724 was able to grow anaerobically on 1,2-propanediol and 1,2-ethanediol as carbon and energy sources. whole cells of the bacterium grown anaerobically on 1,2-propanediol or on glycerol catalyzed conversion of 1,2-diols and aldehydes to the corresponding acids and alcohols. glucose-grown cells also converted aldehydes, but not 1,2-diols, to acids and alcohols. the presence of activities of coenzyme b(12)-dependent diol dehydratase, alcohol dehyd ... | 1979 | 378959 |
| introduction of bacteriophage lambda into cells of klebsiella aerogenes. | we have shown that a mutation in the cro gene of phage lambda greatly reduces zygotic induction. this observation has allowed us to move this phage on an episome into cells of klebsiella aerogenes where it grows as well as in cells of escherichia coli. this technique should allow the introduction of various derivatives of lambda into any organism which is able to receive deoxyribonucleic acid from e. coli. | 1979 | 378984 |
| coliform mastitis--a review. | | 1979 | 379056 |
| mastitis: i. in vitro antimicrobial activity of alkyl amines against mastitic bacteria. | the activities of branched and straight chain amines (10 to 18 carbons chain length) were compared in inhibiting the growth of five microorganisms that cause about 95% of bovine mastitis. three gram-positive (streptococcus agalactiae, streptococcus uberis, staphylococcus aureus) and two gram-negative (escherichia coli, klebsiella pneumoniae) bacteria were used in a trypticase soy broth tube culture growth assay. sixty-two compounds were screened at concentrations of 200, 100, 50, 25, 10, 5, and ... | 1979 | 379061 |
| enterotoxigenic enteric bacteria in foods and outbreaks of food-borne diseases in sweden. | all of 86 food routinely examined for potentially pathogenic enteric bacteria were found to harbour one or more coliform species. none of the strains isolated produced heat-labile enterotoxin (lt) or showed invasive properties. the suckling mouse test indicated that one strain of escherichia coli produced heat-stable enterotoxin (st). twelve incidents of suspected food poisoning were also investigated. in two of them the foods examined contained lt-producing strains of e. coli and in two there w ... | 1979 | 379211 |
| acquisition of klebsiella aerogenes by neonates. | the acquisition of klebsiella aerogenes by neonates born in hospital has been studied by sero- and klebecin typing. this organism was more commonly carried in the bowel of breast-fed babies than of bottle-fed babies. only very rarely did babies acquire strains of k. aerogenes from their mothers. k. aerogenes was widely distributed in the ward environment and on the hands of nurses and mothers. some strains were able to spread on the ward. these results are relevant to the control of k. aerogenes ... | 1979 | 379340 |
| biosynthesis of pullulanase, an outer membrane enzyme in klebsiella. differential effect of translation and transcription inhibitors on synthesis of enzymes in cytoplasm, periplasm, cytoplasmic and outer membrane. | | 1979 | 379591 |
| further studies on the immunohistochemical localization of alpha-msh in the rat brain. | using two different specific antisera directed against alpha-melanocyte-stimulating hormone (alpha-msh), the immunoperoxidase localization of alpha-msh was performed on sections of the rat brain. with one antiserum, some nerve fibers stained for alpha-msh were seen close to the apex of ependymal cells near the lumen of the lateral and third ventricles. with the other antiserum, alpha-msh positive nerve fibers were localized in the pai mater. these results indicate that two different specific ant ... | 1979 | 379713 |
| circular dichroism and magnetic circular dichroism of nitrogenase proteins. | circular dichroism (cd) and magnetic circular dichroism (mcd) spectra of nitrogenase components (mofe protein and fe protein) from azotobacter vinelandii (av) and klebsiella pneumoniae (kp) have been obtained in the near infrared-visible-near ultraviolet spectral region. previously, visible cd was reported to be absent or barely detectable in nitrogenase proteins; mcd spectra have not been reported. the chiroptical spectra can be measured in solution at room temperature, an advantage relative to ... | 1979 | 379860 |
| physical map of chromosomal nitrogen fixation (nif) genes of klebsiella pneumoniae. | we describe a method for the rapid determination of the physical location of mutations caused by insertion of transposable elements. we used this method to construct a detailed physical map of the nitrogen fixation (nif) gene cluster of klebsiella pneumoniae and to correlate it with the genetic map. total cellular dna was isolated from individual strains, each carrying an insertion in 1 of 15 different nif genes. the dna was digested with a restriction endonuclease, fractionated by agarose gel e ... | 1979 | 379866 |
| antibiotic-resistant serratia marcescens infection in a hospital. | over a 12-month period, 74 isolates of serratia marcescens were obtained from various sources at tygerberg hospital. the majority of these isolates were from catheterized patients with urinary tract infections, and were non-pigmented and resistant to all antibiotics tested, excepting amikacin and neomycin. all isolates transferred resistance to tobramycin, gentamicin and tetracycline by conjugation to escherichia coli recipients as separate markers at low frequency. a non-self-transmissible plas ... | 1979 | 380020 |
| acute suppurative thyroiditis. | | 1979 | 380321 |
| clindamycin in treatment of aspiration pneumonia in children. | twenty-eight patients with anaerobic pleuropulmonary infections were treated with clindamycin alone or clindamycin with gentamicin. sixteen of the patients presented with pneumonitis, nine with necrotizing pneumonia, and three with lung abscesses. the average length of treatment was 13.8 days, and the duration of temperature after initiation of therapy was 3.1 days. the predominant isolates were anaerobic gram-positive cocci (23 isolates), bacteroides melaninogenicus (14), bacteroides fragilis ( ... | 1979 | 380459 |
| nosocomial multiply resistant klebsiella pneumoniae: epidemiology of an outbreak of apparent index case origin. | a nosocomial epidemic of multiply resistant (mr) klebsiella pneumoniae characterized by resistance to gentamicin, tobramycin, kanamycin, cephalothin, chloramphenicol, and ampicillin occurred in a veterans administration hospital from 1975 to 1977. a total of 66 infected or colonized patients were observed in a 2-year period; there were 43 urinary tract infections, 13 wound or soft tissue infections, 8 pneumonias, and 6 patients with only asymptomatic stool colonization. four patients had both pn ... | 1979 | 380463 |
| physical characterization of ten r plasmids obtained from an outbreak of nosocomial klebsiella pneumoniae infections. | gentamicin resistance in klebsiella pneumoniae involved in an outbreak at the minneapolis veterans administration hospital was due to a transmissible r plasmid. in addition to gentamicin, this plasmid conferred resistance to tobramycin, kanamycin, ampicillin, carbenicillin, cephalothin, chloramphenicol, and sulfathiazole. r plasmids which transferred this complex antibiogram were identified in several clinical isolates, including four different serotypes of k. pneumoniae, escherichia coli, enter ... | 1979 | 380464 |
| fresh immature articular cartilage allografts. a study on the integration of chondral and osteochondral grafts both in normal and in papain-treated knee joints of rabbits. | the integration of immature cartilage allografts to knee joints of both normal rabbits and those submitted to arthritis through papain was studied for a period of up to 9 months. two types of grafts were compared: chondral--constituted only by cartilage, and osteochondral--cartilage plus a thin layer of subchondral bone. the integration of the grafts was analyzed through inspection of the articular surface and histologic sections. the viability of the grafts was checked through the incorporation ... | 1979 | 380514 |
| stereospecificity and mechanism of adenosylcobalamin-dependent diol dehydratase. catalysis and inactivation with meso- and dl-2,3-butanediols as substrates. | | 1979 | 380560 |
| specificity of the macrophage spreading test with reference to leishmania antigens and correlation with delayed hypersensitivity. | the inhibition of the macrophage spreading test, claimed to be an in vitro correlate of delayed hypersensitivity, was examined in guinea-pigs immunized with l. enriettii and l. tropica soluble antigens. cells from peritoneal washings of the guinea-pigs were tested in presence of the homologous and heterologous antigens and also without antigen. inhibition of macrophage spreading compared to control preparations was noted only in the presence of the homologous antigen when the skin test response ... | 1979 | 380856 |
| [sensitivity of frequent-occurring isolates in outpatients to routinely-used antibiotics (author's transl)]. | the susceptibility of microorganisms (staphylococcus aureus, streptococcus pyogenes, streptococcus pneumoniae, e. coli, klebsiella pneumoniae, salmonella spp. and haemophilus influenzae) frequently occurring in outpatients (treated by practitioners) to six kinds of antibiotics [ampicillin (abpc), cefazolin (cez), erythromycin (em), minocycline (mnc), gentamicin (gm) and thiamphenicol (tp)] was determined by the standard method established by the japan society of chemotherapy. 1) there were few m ... | 1979 | 381708 |
| the peculiar behaviour of coliphage p1vir mutants on restricting hosts. | the sensitivity of two vir coliphage p1 mutants, to the host-specificity systems a, k, rii, and b of escherichia coli, and to the newly identified host-specificity systems mir and 59 of klebsiella pneumoniae has been studied. both 1 vir phages were completely resistant to the e. coli k, but were restricted 10(-5) by the b, mir and 59 systems. the p1 parent, on the contrary, was restricted 10(-2) by all of the above mentioned hosts. the possible role of phage coded proteins in the altered sensiti ... | 1978 | 381845 |
| different effects of the capsular polysaccharide of klebsiella pneumoniae on the functions of various subpopulations of b cells in the immune response of mice to t-dependent antigen. | | 1979 | 381848 |
| a simple method for the separation of tissue-cultured plant cells from unbound bacteria: a demonstration of acquired affinity for bacteria by cells of soy bean. | tissue-cultured cells of soy bean, grown in liquid medium and then transferred to an agar substratum in so-called tissue chambers, showed an affinity for the binding of applied bacteria (klebsiella pneumoniae). this binding, which became greater as the plant cells remained on the agar for up to 10 days, was manifested as an increasing resistance of the bacteria to be washed from the plant cells in a standard, gentle and reproducible procedure devised for this purpose. the number of bacteria firm ... | 1979 | 382698 |
| neonatal lung abscess. a report of six cases. | six infants 8 weeks of age or younger had lung abscesses treated in our medical center during the 20-year period of from 1957 to 1977. the duration of symptoms prior to diagnosis, the etiologic organisms, and the therapeutic approach differ from those in older children and adults. the pathogenic organisms were escherichia coli, group b streptococcus, and klebsiella pneumoniae, initial therapy with a penicillin and an aminoglycoside are indicated until identification and susceptibility results ar ... | 1979 | 382838 |
| [bactericidal kinetics of cefazolin, cefoxitin and amikacin alone and in combination (author's transl)]. | the in vitro activity of cefazolin, cefoxitin und amikacin alone and in combination was determined via membrane filtration against e. coli (n = 2), k. pneumoniae (n = 2) and of cefoxitin and amikacin against proteus vulgaris (n = 1). therapeutically achievable concentrations of cefazolin (10 micrograms/ml), cefoxitin (10 micrograms/ml) and amikacin (10 micrograms/ml) lead to a rapid bactericidal effect against all of our strains. the combination of cefazolin (10 micrograms/ml) and amikactin (5 m ... | 1978 | 383106 |
| hydrogen transfer in catalysis by adenosylcobalamin-dependent diol dehydratase. | studies [bachovchin, w. w., et al. (1978) biochemistry 17, 2218] of the mechanism of inactivation of adenosylcobalamin-dependent diol dehydratase have led to the development of a general method to describe the kinetics of a reaction pathway containing a reservoir of mobile hydrogen. analysis by this method of catalytic rate measurements for mixtures of 1,2-propanediol and 1,1-dideuterio-1,2-propanediol supports a mechanism involving an intermediate with three equivalent hydrogens, in which hydro ... | 1979 | 383139 |
| resolution of the coenzyme b-12-dependent dehydratases of klebsiella sp. and citrobacter freundii. | diol dehydratase (1,2-propanediol hydro-lyase, ec 4.2.1.28) and glycerol dehydratase (glycerol hydro-lyase, ec 4.2.1.30) are shown to be distinct, separable enzymes that occur individually or together in different strains of klebsiella sp. anaerobic growth with propan-1,2-diol induces diol dehydratase alone, whereas glycerol fermentation induces both enzymes in k. pneumoniae atcc 25955 and in citrobacter freundii ncib 3735. the dehydratases can be resolved by polyacrylamide-gel electrophoresis o ... | 1979 | 383154 |
| osteopetrosis reconsidered as a curable immune disorder. | osteopetrosis is a unique model for investigating osteoclast formation from lymphocytes and more precisely from t-lymphocytes. in the pathogenesis of this condition, a thymic impairement must be postulated. the cure of several osteopetrotic animal mutants and an infant suffering from juvenile malignant osteopetrosis by injecting normal marrow are the first examples of the successful management of a bone disorder by cell injection. | 1979 | 383170 |
| the coupling of electron transfer in nitrogenase to the hydrolysis of magnesium adenosine triphosphate. | | 1979 | 383544 |
| the lactose system in klebsiella aerogenes v9a. 6. lactose transport. | | 1979 | 383575 |
| comparison of assay of coliform enterotoxins by conventional techniques versus in vivo intestinal perfusion. | thirty-six strains of coliform bacteria were tested for enterotoxigenicity both by conventional assays, including the y-1 adrenal and chinese hamster ovary cell assays for heat-labile toxin and the suckling mouse assay for heat-stable toxin, and by determining the ability of graded concentrations of ultrafiltrate high- or low-molecular-weight toxin preparations to induce water secretion during in vivo perfusion in the rat jejunum. the ultrafiltrates of all 18 strains isolated from persons with i ... | 1979 | 383611 |
| comparison of disc zone sizes with minimum inhibitory concentrations using semisynthetic penicillin. | | 1979 | 383681 |