Publications

risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry.	the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ...	2017	27478029
identification and growth dynamics of meat spoilage microorganisms in modified atmosphere packaged poultry meat by maldi-tof ms.	modified atmosphere packaging (map) is widely used in food industry to extend the microbiological shelf-life of meat. typically, poultry meat has been packaged in a co2/n2 atmosphere (with residual low o2). recently, some producers use high o2 map for poultry meat to empirically reach comparable shelf lifes. in this work, we compared spoilage microbiota of skinless chicken breast in high (80% o2, 20% co2) and low o2 map (65% n2 and 35% co2). two batches of meat were incubated in each atmosphere ...	2016	27554149
delayed-onset postoperative endophthalmitis caused by hafnia alvei.	to identify hafnia alvei as an etiological factor of delayed-onset endophthalmitis.	2017	28226154
human norovirus binding to select bacteria representative of the human gut microbiota.	recent reports describe the ability of select bacterial strains to bind human norovirus, although the specificity of such interactions is unknown. the purpose of this work was to determine if a select group of bacterial species representative of human gut microbiota bind to human norovirus, and if so, to characterize the intensity and location of that binding. the bacteria screened included naturally occurring strains isolated from human stool (klebsiella spp., citrobacter spp., bacillus spp., e ...	2017	28257478
group ii intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer hafnia alvei ad27.	our previous studies showed that glycerol fermentation by hafnia alvei ad27 strain was accompanied by formation of high quantities of lactate. the ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. group ii intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of h. alvei ad27 strain was conducted. the effect of the δldh genotype in h. alvei ad27 strain varied depending on the culture medium a ...	2017	28284024
screening for fecal carriage of mcr-producing enterobacteriaceae in healthy humans and primary care patients.	the extent of the occurrence of the plasmid-encoded colistin resistance genes mcr-1 and mcr-2 among humans is currently sparsely studied in western europe.	2017	28316780
characteristics of n-acylhomoserine lactones produced by hafnia alvei h4 isolated from spoiled instant sea cucumber.	this study aimed to identify n-acylhomoserine lactone (ahl) produced by hafnia alvei h4, which was isolated from spoiled instant sea cucumber, and to investigate the effect of ahls on biofilm formation. two biosensor strains, chromobacterium violaceum cv026 and agrobacterium tumefaciens kyc55, were used to detect the quorum sensing (qs) activity of h. alvei h4 and to confirm the existence of ahl-mediated qs system. thin layer chromatography (tlc) and high resolution triple quadrupole liquid chro ...	2017	28379194
bacterial communities of fresh goat meat packaged in modified atmosphere.	the objective of this work was to study the growth and development of fortuitous flora and food pathogens in fresh goat meat packaged under modified atmospheres containing two different concentrations of co2. meat samples were stored at 10 °c under two different modified-atmosphere packing (map) conditions: treatment a had 45% co2 + 20% o2 + 35% n2 and treatment b had 20% co2 + 55% o2 + 25% n2. during 14 days of storage, counts of each bacterial group and dominant species identification by 16s r ...	2017	28400020
inhibition of hafnia alvei h4 biofilm formation by the food additive dihydrocoumarin.	quorum sensing (qs) is an intercellular signaling and gene regulatory mechanism that is implicated in food spoilage caused by bacteria. thus, blocking qs may suppress qs-controlled phenotypes of these bacteria that are responsible for food spoilage. biofilm formation is closely related to bacterial infection, and it is also a major mechanism responsible for the increased resistance of biofilm-associated bacteria to antimicrobial drugs. food spoilage and biofilm formation caused by food-related b ...	2017	28402186
[a descriptive study of hafnia alvei isolated from stool samples: an approach to its clinical assessment].	the importance in human diarrhoeal disease of hafnia alvei is unclear. the objective of the study was to describe the population which was isolated h. alvei in stool cultures and the therapeutic management of these cases in our health area.	2017	28537065
multiple microbial cell-free extracts improve the microbiological, biochemical and sensory features of ewes' milk cheese.	this study used cell-free enzyme (cfe) extracts from lactobacillus casei, hafnia alvei, debaryomyces hansenii and saccharomyces cerevisiae to condition or accelerate pecorino-type cheese ripening. compositional, microbiological, and biochemical analyses were performed, and volatile and sensory profiles were obtained. lactobacilli and cocci increased during ripening, especially in cheeses containing cfe from l. casei, h. alvei and d. hansenii (lhd-c) and l. casei, h. alvei and s. cerevisiae (lhs- ...	2017	28576361
hafnia, an enterobacterial genus naturally resistant to colistin revealed by three susceptibility testing methods.	to determine the susceptibility to colistin of hafnia alvei and hafnia paralvei, and to compare methods for colistin resistance detection in the hafnia genus.	2017	28586429
honeybee (apis mellifera)-associated bacterial community affected by american foulbrood: detection of paenibacillus larvae via microbiome analysis.	honeybee (apis mellifera l.) workers act as passive vectors of paenibacillus larvae spores, which cause the quarantine disease american foulbrood (afb). we assessed the relative proportions of p. larvae within the honeybee microbiome using metabarcoding analysis of the 16 s rrna gene. the microbiome was analyzed in workers outside of the afb zone (control - afb0), in workers from asymptomatic colonies in an afb apiary (afb1), and in workers from colonies exhibiting clinical afb symptoms (afb2). ...	2017	28698604
microorganisms of the intestinal microbiota of oncorhynchus mykiss produce antagonistic substances against bacteria contaminating food and causing disease in humans.	the objective was to analyse the antibacterial ability of members of the intestinal microbiota of oncorhynchus mykiss specimens on several bacterial target strains that contaminate food and cause disease in humans. bacterial colonies from an intestinal portion of the 20 specimens of o. mykiss were obtained in different culture media. several of the colonies showed antibacterial action on different target strains. the bacterial species with the highest antagonistic capacity were hafnia alvei and ...	2017	28713788
microbial cell-free extracts affect the biochemical characteristics and sensorial quality of sourdough bread.	this study aimed to improve the sensorial quality of sourdough wheat bread by the addition of cell-free enzyme extracts (cfes) from lactobacillus sanfranciscensis (sf), hafnia alvei (hf) and debaryomyces hansenii (dh). cfes were suitable sources of peptidases, glutamate dehydrogenase and cystathionine γ-lyase. the concentration of free amino acids (faa) in the sourdoughs containing cfes was higher than the control sourdough, produced without addition of cfes. the community-level catabolic profil ...	2017	28763982
complete genome sequence of a commensal bacterium, hafnia alvei cba7124, isolated from human feces.	members of the genus hafnia have been isolated from the feces of mammals, birds, reptiles, and fish, as well as from soil, water, sewage, and foods. hafnia alvei is an opportunistic pathogen that has been implicated in intestinal and extraintestinal infections in humans. however, its pathogenicity is still unclear. in this study, we isolated h. alvei from human feces and performed sequencing as well as comparative genomic analysis to better understand its pathogenicity.	2017	28770009