| facile synthesis of 2-[(3-aminopropyl)thio]adenosine 5'-diphosphate: a key intermediate for the synthesis of molecular probes of adenosine 5'-diphosphate function. | adenosine 5'-diphosphate (adp) and, uniquely, its c-2 derivatized analogues are able to induce platelet activation. we here report the synthesis of 2-[(3-aminopropyl)thio]-adp from adp itself via 1,n6-etheno-adp. 2-[(3-aminopropyl)thio]-adp induced platelet aggregation with a potency about one-seventh that of adp itself and should prove a useful intermediate in the synthesis of other probes of platelet function. | 2013 | 3669009 |
| [individuation and communication in various neurosis structures]. | | 2015 | 4692550 |
| [primary surgical treatment of punctate wounds in open fractures of the crural bones]. | | 2015 | 4610479 |
| [300th anniversary of lucka pharmacy]. | | 2016 | 4931013 |
| [2 cases of upper caval vein anomaly (diagnostic difficulties in mediastinal tumors)]. | | 2015 | 4774754 |
| [systole phase and cardiac volume dynamics in dosed veloergometric loading in patients with ischemic heeart disease and its significance in detecting pre-clinical stage of cardiac insufficiency]. | | 2016 | 5043024 |
| mental health: systems and non-systems. | | 2015 | 4357958 |
| genetics and schizophrenia. | | 2016 | 4739404 |
| report on injuries sustained by patients treated at the birmingham general hospital following the recent bomb explosions. | as a result of recent bomb explosions a total of 82 patients were treated at the birmingham general hospital, 61 with minor injuries. bomb injuries may be divided into three main groups due to the blast effect (such as blast lung and ruptured tympanic membranes), the flash (such as burns to the exposed part of the body), and shrapnel (which may cause a wide variety of injuries). the amount of warning of such explosions is usually minimal, and so the prepared accident schemes of most hospitals ar ... | 2005 | 1169088 |
| [disorders in the sequence of regional metastasis and long-term results of the surgical treatment of complicated lung cancer]. | to ascertain the frequency of disturbance of the stage character of regional metastasis in lung carcinoma complicated by disintegration or obstructive pneumonitis, and its influence on certain results of surgical treatment, the authors analysed the data on 175 patients who underwent lung resection. the stage character of regional lymphogenous metastasis was found to be disturbed in 61 (34.8%) patients, in 52 (85.2%) of them the main disease was complicated by obstructive pneumonitis. five-year s ... | 2007 | 2223212 |
| [disorders in the sequence of regional metastasis and long-term results of the surgical treatment of complicated lung cancer]. | to ascertain the frequency of disturbance of the stage character of regional metastasis in lung carcinoma complicated by disintegration or obstructive pneumonitis, and its influence on certain results of surgical treatment, the authors analysed the data on 175 patients who underwent lung resection. the stage character of regional lymphogenous metastasis was found to be disturbed in 61 (34.8%) patients, in 52 (85.2%) of them the main disease was complicated by obstructive pneumonitis. five-year s ... | 2007 | 2223212 |
| [analysis of kinetics of consecutive metabolic cerebral activations induced by spatial discrimination testing, using (14c)-glucose, in an eight-arm radial maze in mice]. | regional mapping of relative (14c)-glucose (glu) uptake was analyzed in balb/c mice at 3 time intervals (5 min., 1 hr., 3 hrs.) after either the first (day 1) or the last (day 9) daily sessions of a spatial discrimination testing procedure in an eight-arm radial maze. on day 1, increased labelling was found 5 min. post-training in subcortical, hippocampal and cortical regions. decreased glu uptake was observed 1 hr. later in the same regions, followed at 3 hrs. post-training by a retarded activa ... | 2007 | 1913258 |
| the prognostic value of exercise capacity: a review of the literature. | while there is still much debate in the literature regarding the specific met levels at which there are differences in survival, the following points have become clear with the growing body of reports in the literature. exercise capacity seems to be an independent predictor of mortality, and when it is combined with other clinical, exercise, or angiographic data, it becomes very powerful in this regard. this relates to both overall mortality and to that from cardiovascular disease. there is stil ... | 2007 | 1951007 |
| [thermometric diagnostics of chronic ulcerous pulpitis]. | thermometric measurements were performed of 408 teeth of the upper and lower jaw with clinically confirmed initial and advanced stage of chronic ulcerous pulpitis in 398 subjects, aged from 18 to 30. temperature elevation within the range from 1.4 to 3.2 degrees c was established in the initial form of chronic pulpitis and from 1 to 2.5 degrees c in advanced form of chronic pulpitis as compared with the norm. significant difference in the temperature deviations exists in both forms of chronic pu ... | 2007 | 2562574 |
| evaluation of n-(5-indanylsulfonyl)-n'-(4-chlorophenyl)-urea against xenografts of pediatric rhabdomyosarcoma. | n-(5-indanylsulfonyl)-n'-(4-chlorophenyl)-urea (ly186641), a novel anticancer compound, was evaluated against six lines of rhabdomyosarcoma xenografts, each of which was established from tissue biopsies from untreated patients, and additional sublines selected as xenografts for primary resistance to vincristine, melphalan, and ifosfamide. ly186641 was given by oral gavage twice daily for 10 consecutive days or as 5-day courses repeated at 7-day intervals. at the optimal schedule, complete regres ... | 2008 | 2598403 |
| stefan kopeć 1888-1941. | | 2010 | 3321899 |
| orbital apex syndrome due to sickle cell anemia. | | 2010 | 3153230 |
| excessive atp degradation during hemodialysis against sodium acetate. | as the initial step in examining the metabolic basis for acetate intolerance, we have tested the hypothesis that excessive adenosine triphosphate (atp) degradation occurs during hemodialysis against acetate dialysate (compared with the degree of degradation occurring during dialysis against bicarbonate dialysate). seven patients undergoing long-term dialysis were infused with carbon 14--labeled 8-adenine, and their response to dialysis against acetate was compared with their response to dialysis ... | 2011 | 3193024 |
| [ileocecal actinomycosis as a complication of crohn disease. clinicopathologic aspects]. | | 2011 | 3175218 |
| ventricular arrhythmias in normal individuals and athletes. | | 2011 | 3042408 |
| five-year results and complications with hdr afterloading in cervix cancer using a linear arrangement of source. | | 2011 | 3134705 |
| lower levels of vitamin c and carotenes in plasma of cigarette smokers. | plasma levels of vitamins a, c, and e, selenium, and carotenes were determined in 125 male cigarette smokers and 125 age- and race-matched nonsmokers. the smokers had a mean daily consumption of 30.6 cigarettes and a cumulative consumption of 22.8 pack years. plasma levels of vitamin c and total carotenes were significantly (p less than 0.05) lower in smokers than those of nonsmokers, while levels of vitamin a, selenium, and vitamin e were not significantly different between these two groups. si ... | 2013 | 3734276 |
| [bacteriuria: importance and value of the semi-quantitative method of uricult. comparative study]. | | 2013 | 4553474 |
| usefulness of an implantable antitachycardia pacemaker system for supraventricular or ventricular tachycardia. | the cordis omni-orthocor model 234a, an implantable antitachycardia system, was evaluated in 13 patients. two patients had recurrent sustained supraventricular tachycardia (svt) and 11 had ventricular tachycardia (vt). the system was used for svt or vt termination (group 1: svt, 2 patients; vt, 4 patients) or for demand pacing and noninvasive electrophysiologic studies for tachycardia induction and serial electrophysiologic testing alone (group 2: vt, 7 patients). the overdriver was used success ... | 2013 | 3728334 |
| sequential isokinetic and manual muscle testing in patients with neuromuscular disease. a pilot study. | the purposes of this study were to compare the value of isokinetic testing and manual muscle testing (mmt) in longitudinal measurements of muscle strength in patients with neuromuscular disease and to identify any consistent pattern demonstrated by the isokinetic testing of patients with specific diagnoses. we measured knee extensor muscle strength at periodic intervals in patients, using mmt and isokinetic testing at angular limb velocities of 30 and 180 degrees/sec. an isokinetic fatigability ... | 2013 | 3941825 |
| alteration of the protein composition in the haemolymph of american cockroaches immunized with soluble proteins. | previous in vivo experiments demonstrating the existence of humoral immunity in cockroaches prompted us to investigate the composition and number of proteins that are newly induced or increased in quantity in the haemolymph of immune cockroaches compared to saline-injected control animals. sds-page analysis showed that six different haemolymph proteins of molecular weights 220,000, 162,000, 115,000, 102,000, 95,000 and 45,000 were induced in response to immunization by honeybee toxoid. however, ... | 2013 | 3666788 |
| [d-penicillamine induced lupus during the treatment of rheumatoid arthritis. report of 2 cases and systematic immunologic study during the treatment]. | | 2014 | 4136184 |
| blue cones contribute to border distinctness. | a conclusion reached by kaiser and boynton in wavelength discrimination experiments, that differential blue-cone excitation makes a small contribution to the distinctness of borders in small fields, was verified using a rating procedure for borders produced in the la jolla analytic colorimeter. | 2014 | 4072017 |
| 5-aminosalicylic acid for the treatment of inflammatory bowel diseases. | | 2014 | 4018510 |
| validity of an empirically derived phenomenological typology. | | 2016 | 5071939 |
| clinical problems encountered in renal homotransplantation in children. | | 2016 | 4911478 |
| [centenary of the birth of mining engineer i. m. puginov]. | | 2016 | 4901215 |
| chemotherapy with hydroxyurea (nsc-32065) in renal cell carcinoma. | | 2016 | 4883783 |
| control rate in diphenylhydantoin therapy. | | 2016 | 4717597 |
| conjoint scaling of subjective number and weight. | | 2016 | 4705240 |
| dectin-2 recognizes mannosylated o-antigens of human opportunistic pathogens and augments lipopolysaccharide activation of myeloid cells. | lps consists of a relatively conserved region of lipid a and core oligosaccharide and a highly variable region of o-antigen polysaccharide. whereas lipid a is known to bind to the toll-like receptor 4 (tlr4)-myeloid differentiation factor 2 (md2) complex, the role of the o-antigen remains unclear. here we report a novel molecular interaction between dendritic cell-associated c-type lectin-2 (dectin-2) and mannosylated o-antigen found in a human opportunistic pathogen, hafnia alvei pcm 1223, whic ... | 2016 | 27358401 |
| genetic diversity of o-antigens in hafnia alvei and the development of a suspension array for serotype detection. | hafnia alvei is a facultative and rod-shaped gram-negative bacterium that belongs to the enterobacteriaceae family. although it has been more than 50 years since the genus was identified, very little is known about variations among hafnia species. diversity in o-antigens (o-polysaccharide, ops) is thought to be a major factor in bacterial adaptation to different hosts and situations and variability in the environment. antigenic variation is also an important factor in pathogenicity that has been ... | 2016 | 27171009 |
| quorum sensing activity of hafnia alvei isolated from packed food. | quorum sensing (qs) is a mechanism adopted by bacteria to regulate expression of genes according to population density. n-acylhomoserine lactones (ahls) are a type of qs signalling molecules commonly found in gram-negative bacteria which have been reported to play a role in microbial spoilage of foods and pathogenesis. in this study, we isolated an ahl-producing hafnia alvei strain (fb1) from spherical fish pastes. analysis via high resolution triple quadrupole liquid chromatography/mass spectro ... | 2014 | 24736131 |
| fifth stenhous-williams memorial lecture. oxygen and the obligate anaerobe. | | 1981 | 181358 |
| [autoregulation of l-asparaginase-induced diabetes mellitus]. | | 1991 | 270391 |
| [immunofluorescent study of the distribution of adult neuro-specific antigens in the chick embryo (author's transl)]. | the adult neuro-specific antigens have been localized by immunofluorescence techniques in diencephalon and mesencephalon of chick embryo. this study has been made using fresh or fixed tissues from embryos 72, 48 or 36 h old. at 72 h of incubation the wall of diencephalon shows marked fluorescence; at 48 h of incubation the fluorescent cells are localized in an outer layer and an inner one. in the 48 h-old embryo the reaction is more distinct and intensive in fresh tissues than in fixed tissues. ... | 1991 | 328815 |
| molecular characteristics of extended-spectrum cephalosporin-resistant enterobacteriaceae from humans in the community. | to investigate the molecular characteristics of extended-spectrum cephalosporin (esc)-resistant enterobacteriaceae collected during a cross-sectional study examining the prevalence and risk factors for faecal carriage of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in humans living in areas with high or low broiler density. | 2015 | 26029910 |
| occurrence of urinary tract infection in adolescent and adult women of shanty town in dhaka city, bangladesh. | urinary tract infection (uti) is commonly experienced by women of various age groups especially elderly ones. we planned to find out the prevalent microbial strains causing uti in slum inhabitant adolescent and adult women in dhaka city, bangladesh. | 2014 | 24795516 |
| hafnia alvei urosepsis in a kidney transplant patient. | hafnia alvei, a gram-negative facultative anaerobic, rod-shaped bacterium, is a rare cause of infection in humans. we report on a renal transplant patient who developed h. alvei pyelonephritis and urosepsis. the source of infection remains enigmatic but is most likely the intestinal tract. appropriate antibiotic therapy with cefepime followed by oral ciprofloxacin brought about rapid resolution of symptoms and complete recovery. h. alvei may cause severe infection in transplant patients without ... | 2015 | 25960912 |
| draft genome sequence of hafnia paralvei strain gta-haf03. | hafnia paralvei is a gram-negative member of the enterobacteriaceae family, closely related to the opportunistic pathogen hafnia alvei. we report here the first draft genome sequence of h. paralvei, from the beef trim isolate gta-haf03, consisting of a 5.0-mbp assembly encoding 4,382 proteins and 90 predicted rnas. | 2015 | 25700416 |
| antibiotic resistance of gram-negative benthic bacteria isolated from the sediments of kardzhali dam (bulgaria). | the aim of the present study was to carry out a preliminary assessment for the occurrence of bacterial strains resistant to frequently used antibiotics in the sediments beneath the sturgeon cage farm in kardzhali dam (bulgaria). samples were taken from the top 2 cm of sediments under a fish farm and from a control station in the aquatory of the reservoir in the period july-october 2011. surveillance of bacterial susceptibility to 16 antimicrobial agents was performed for 160 gram-negative strain ... | 2015 | 26019641 |
| whole-genome sequence of hafnia alvei humv-5920, a human isolate. | a clinical isolate of hafnia alvei (strain humv-5920) was obtained from a urine sample from an adult patient. we report here its complete genome assembly using pacbio single-molecule real-time (smrt) sequencing, which resulted in a chromosome with 4.5 mb and a circular contig of 87 kb. about 4,146 protein-coding genes are predicted from this assembly. | 2016 | 27313299 |
| genome sequence of hafnia alvei bta3_1, a bacterium with antimicrobial properties isolated from honey bee gut. | hafnia alvei bta3_1, a strain with antibacterial properties, was isolated from honey bee gut and cultured under aerobic and anaerobic conditions. to explore the potential genetic bases of its antibacterial and possible pathogenic properties, the complete genome of this organism was sequenced and analyzed. | 2016 | 27284146 |
| a new ligand-based method for purifying active human plasma-derived ficolin-3 complexes supports the phenomenon of crosstalk between pattern-recognition molecules and immunoglobulins. | despite recombinant protein technology development, proteins isolated from natural sources remain important for structure and activity determination. ficolins represent a class of proteins that are difficult to isolate. to date, three methods for purifying ficolin-3 from plasma/serum have been proposed, defined by most critical step: (i) hydroxyapatite absorption chromatography (ii) n-acetylated human serum albumin affinity chromatography and (iii) anti-ficolin-3 monoclonal antibody-based affini ... | 2016 | 27232184 |
| chemical composition and antibacterial activity of lavandula coronopifolia essential oil against antibiotic-resistant bacteria. | the aim of this study was to analyse the composition of the essential oil (eo) of lavandula coronopifolia from morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. the antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: klebsiella pneumoniae subsp. pneumoniae, klebsiella ornithinolytica, escherichia coli, enterobacter cloacae, ent ... | 2015 | 25174508 |
| evaluating the occurrence of escherichia albertii in chicken carcass rinses by pcr, vitek analysis, and sequencing of the rpob gene. | escherichia albertii is a recently described species that has been associated with gastroenteritis in humans and with healthy and ill birds. most recently, it has been identified as the causative agent in a food-borne outbreak in japan. the distribution and clinical importance of e. albertii are not well studied because its importance is unclear. culture methods for clinical isolation frequently miss e. albertii or incorrectly identify it as shigella spp., escherichia coli, or hafnia alvei. this ... | 2015 | 25548040 |
| degradation of phytate by the 6-phytase from hafnia alvei: a combined structural and solution study. | phytases hydrolyse phytate (myo-inositol hexakisphosphate), the principal form of phosphate stored in plant seeds to produce phosphate and lower phosphorylated myo-inositols. they are used extensively in the feed industry, and have been characterised biochemically and structurally with a number of structures in the pdb. they are divided into four distinct families: histidine acid phosphatases (hap), β-propeller phytases, cysteine phosphatases and purple acid phosphatases and also split into thre ... | 2013 | 23741456 |
| characteristics of n-acylhomoserine lactones produced by hafnia alvei h4 isolated from spoiled instant sea cucumber. | this study aimed to identify n-acylhomoserine lactone (ahl) produced by hafnia alvei h4, which was isolated from spoiled instant sea cucumber, and to investigate the effect of ahls on biofilm formation. two biosensor strains, chromobacterium violaceum cv026 and agrobacterium tumefaciens kyc55, were used to detect the quorum sensing (qs) activity of h. alvei h4 and to confirm the existence of ahl-mediated qs system. thin layer chromatography (tlc) and high resolution triple quadrupole liquid chro ... | 2017 | 28379194 |
| gene clusters of hafnia alvei strain fb1 important in survival and pathogenesis: a draft genome perspective. | hafnia alvei is an opportunistic pathogen involved in various types of nosocomical infections. the species has been found to inhabit food and mammalian guts. however, its status as an enteropathogen, and whether the food-inhabiting strains could be a source of gastrointestinal infection remains obscure. in this report we present a draft genome of h. alvei strain fb1 isolated from fish paste meatball, a food popular among malaysian and chinese populations. the data was generated on the illumina m ... | 2014 | 25075225 |
| pyrosequencing analysis of microbial community and food-borne bacteria on restaurant cutting boards collected in seri kembangan, malaysia, and their correlation with grades of food premises. | this study adopts the pyrosequencing technique to identify bacteria present on 26 kitchen cutting boards collected from different grades of food premises around seri kembangan, a city in malaysia. pyrosequencing generated 452,401 of total reads of otus with an average of 1.4×10(7) bacterial cells/cm(2). proteobacteria, firmicutes and bacteroides were identified as the most abundant phyla in the samples. taxonomic richness was generally high with >1000 operational taxonomic units (otus) observed ... | 2015 | 25679309 |
| comparison of identification systems for psychrotrophic bacteria isolated from raw bovine milk. | psychrotrophic bacteria in raw milk produce heat-resistant extracellular proteases, resulting in spoilage and shelf-life reduction of ultrahigh temperature treated milk and milk products. controlling of these spoilage microbes requires rapid and reliable identification systems for screening of raw milk. this study aimed to compare commercial bacterial identification systems with a genetic method (considered as the 'gold standard' method) for the identification of heat-resistant protease producin ... | 2014 | 25113043 |
| periodontal-disease-associated biofilm: a reservoir for pathogens of medical importance. | the ecological diversity of the periodontal microenvironment may provide suitable conditions for the colonization of species not usually considered members of the oral microbiota. in this investigation, we aimed to determine the prevalence and levels of pathogenic species of medical relevance in the microbiota of individuals with distinct periodontal clinical status. subgingival biofilm was obtained from patients with periodontal health (h, n = 81), gingivitis (g, n = 55), generalized aggressive ... | 2016 | 26416306 |
| risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry. | the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ... | 2017 | 27478029 |
| antimicrobial resistance patterns of enterobacteriaceae in european wild bird species admitted in a wildlife rescue centre. | wild birds have been considered to be reservoirs of enteric human pathogens and vectors of resistance dissemination to the environment. during annual migration, they potentially play a role in the epidemiology of human associated zoonoses. the aim of this study was to investigate the frequency of isolation and antimicrobial susceptibility profiles of enterobacteriaceae members isolated from cloacal swabs of common european wild birds. fifty-five cloacal swabs were taken during birds' entrance ev ... | 2016 | 27393875 |
| application of bacteriophages to reduce biofilms formed by hydrogen sulfide producing bacteria on surfaces in a rendering plant. | hydrogen sulfide producing bacteria (spb) in raw animal by-products are likely to grow and form biofilms in the rendering processing environments, resulting in the release of harmful hydrogen sulfide (h2s) gas. the objective of this study was to reduce spb biofilms formed on different surfaces typically found in rendering plants by applying a bacteriophage cocktail. using a 96-well microplate method, we determined that 3 spb strains of citrobacter freundii and hafnia alvei are strong biofilm for ... | 2015 | 26102989 |
| prevalence and characterization of esbl- and ampc-producing enterobacteriaceae on retail vegetables. | in total 1216 vegetables obtained from dutch stores during 2012 and 2013 were analysed to determine the prevalence of 3rd-generation cephalosporin (3gc) resistant bacteria on soil-grown fresh produce possibly consumed raw. vegetables grown conventionally and organically, from dutch as well as foreign origin were compared. included were the following vegetable types; blanched celery (n=192), bunched carrots (n=190), butterhead lettuce (n=137), chicory (n=96), endive (n=188), iceberg lettuce (n=19 ... | 2015 | 25828704 |
| antibacterial susceptibility of enterobacteriaceae isolated from raw horsemeat isolated for human consumption (basashi). | drug susceptibility testing was carried out using 14 antibiotics in order to identify trends in the antibiotic tolerance of 142 strains of enterobacteriaceae isolated from horsemeat commercially available for raw consumption (basashi). a comparison of the sensitivity to the 14 antibiotics using the 90% mic (minimum inhibitory concentration) values (mic90) showed the strongest tolerance to ampicillin (abpc) at a concentration of > 128 μg/ml, followed by that to fosfomycin (fom) at a concentration ... | 2015 | 25817809 |
| conversion of glycerol to 1,3-propanediol by citrobacter freundii and hafnia alvei - newly isolated strains from the enterobacteriaceae. | in this study, nearly 4000 bacterial strains from the family of enterobacteriaceae isolated from different environments were screened for ability to convert glycerol to 1,3-propanediol (1,3-pd). the aim of the research was to isolate 1,3-pd producers from the natural environment, identify and characterize the best isolates. three selective media were tested to usefulness in the isolation of bacteria from the family enterobacteriaceae. only, 28% of examined isolates could synthesize 1,3-pd from g ... | 2014 | 24768868 |
| [comparison of routine use of two chromogenic media chromid cps (biomérieux) and uriselect4 (bio-rad) for the detection of escherichia coli and major uropathogenics in urine]. | escherichia coli is the most common bacterial cause of urinary tract infections. its rapid and specific identification in urine samples represents a major challenge within the rendering results and optimizing the management of the patient. we aimed to compare the sensitivity and specificity of two commercially available chromogenic media for e. coli: chromid cps (biomérieux) and uriselect4 (bio(-)rad), without carrying out further tests. 99 consecutive and non-redundant urine samples considered ... | 2014 | 24736143 |
| isolation and characterization of bacteriophages specific to hydrogen-sulfide-producing bacteria. | the objectives of this study were to isolate and characterize bacteriophages specific to hydrogen-sulfide-producing bacteria (spb) from raw animal materials, and to develop a spb-specific bacteriophage cocktail for rendering application. meat, chicken offal, and feather samples collected from local supermarkets and rendering processing plants were used to isolate spb (n = 142). bacteriophages (n = 52) specific to spb were isolated and purified from the above samples using 18 of those isolated sp ... | 2013 | 23391228 |
| identification of the enterobacteriaceae in montasio cheese and assessment of their amino acid decarboxylase activity. | the aim of the study was to identify the species of enterobacteriaceae present in montasio cheese and to assess their potential to produce biogenic amines. plate count methods and an enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) approach, combined with 16s rdna sequencing, were used to investigate the enterobacteriaceae community present during the cheesemaking and ripening of 6 batches of montasio cheese. additionally, the potential decarboxylation abiliti ... | 2013 | 23298547 |
| the bactericidal activity of normal human serum against enterobacteriaceae rods with lipopolysaccharides possessing o-antigens composed of mannan. | the susceptibility of bacteria to the bactericidal activity of serum depends on the structure and organization of the bacterial outer membrane. it is known that the structure of the o-specific polysaccharide chain of lipopolysaccharide (lps) plays an important role in the resistance of bacteria to host immune defenses. | 2012 | 23214191 |
| [gram-negative bacteria influence on the ability of the arginine-vasotocin to stimulate osmotic permeability of the frog urinary bladder epithelium]. | the influence of endogenous gram-negative bacteria colonizing the mucosal epithelium of frog rana temporaria l. urinary bladders (fub) on arginine-vasotocin avt-stimulated osmotic water flow in isolated urinary bladders was investigated. 170 animals were examined and only 40% were contaminated with gram-negative bacteria (about 10(3)-10(6) cfu per hemibladder). several enterobacteriaceae species were identified (hafnia alvei, 36.7%, e. coli, 32.3%, serratia marcescens, 8.8%, citrobacter freundii ... | 2012 | 22834340 |
| increased iga and igm responses against gut commensals in chronic depression: further evidence for increased bacterial translocation or leaky gut. | recently, we discovered that depression is accompanied by increased igm and iga responses directed against gram negative gut commensals. the aim of this study was to replicate these findings in a larger study group of depressed patients and to examine the associations between the iga and igm responses to gut commensals and staging of depression as well as the fatigue and somatic (f&s) symptoms of depression. | 2012 | 22410503 |
| microbial diversity and dynamics during the production of may bryndza cheese. | diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ... | 2014 | 24291178 |
| aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland. | we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ... | 2015 | 25380369 |
| survival of cheese-ripening microorganisms in a dynamic simulator of the gastrointestinal tract. | a mixture of nine microorganisms (six bacteria and three yeasts) from the microflora of surface-ripened cheeses were subjected to in vitro digestive stress in a three-compartment "dynamic gastrointestinal digester" (didgi). we studied the microorganisms (i) grown separately in culture medium only (ii) grown separately in culture medium and then mixed, (iii) grown separately in culture medium and then included in a rennet gel and (iv) grown together in smear-ripened cheese. the yeasts geotrichum ... | 2016 | 26611167 |
| the effect of reduced sodium chloride content on the microbiological and biochemical properties of a soft surface-ripened cheese. | many health authorities have targeted salt reduction in food products as a means to reduce dietary sodium intake due to the harmful effects associated with its excessive consumption. in the present work, we evaluated the effect of reducing sodium chloride (nacl) content on the microbiological and biochemical characteristics of an experimental surface-ripened cheese. a control cheese (1.8% nacl) and a cheese with a reduced nacl content (1.3% nacl) were sampled weekly over a period of 27d. reducin ... | 2016 | 26852809 |
| microbial cell-free extracts as sources of enzyme activities to be used for enhancement flavor development of ewe milk cheese. | freeze-dried cell-free extracts (cfe) from lactobacillus casei lc01, weissella cibaria 1xf5, hafnia alvei moller atcc 51815, and debaryomyces hansenii lcf-558 were used as sources of enzyme activities for conditioning the ripening of ewe milk cheese. compared with control cheese (cc), cfe did not affect the gross composition and the growth of the main microbial groups of the cheeses. as shown through urea-page electrophoresis of the ph 4.6-soluble nitrogen fraction and the analysis of free aa, t ... | 2015 | 26142846 |
| identification of enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the vitek ms system. | this multicenter study evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry identifications from the vitek ms system (biomérieux, marcy l'etoile, france) for enterobacteriaceae typically encountered in the clinical laboratory. enterobacteriaceae isolates (n = 965) representing 17 genera and 40 species were analyzed on the vitek ms system (database v2.0), in accordance with the manufacturer's instructions. colony growth (≤72 h) was app ... | 2013 | 23818163 |
| hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol production. | study presented here demonstrates the ability of three newly isolated strains, obtained from environmental probes (manure, bottom sediment, and food waste) and identified as clostridium bifermentans, clostridium butyricum, and hafnia alvei, to synthesize 1,3-propanediol (1,3-pd), organic acids (such as lactic, acetic, fumaric, succinic, and butyric acids), and ethanol from glycerol. the production of 1,3-pd as well as the glycerol pathways in c. bifermentans and h. alvei cells have not been inve ... | 2014 | 25473655 |
| isolation and identification of enterobacteriaceae from raw horsemeat intended for human consumption (basashi). | the status of enterobacteriaceae contamination was investigated in a total of 131 samples of raw horsemeat (basashi) intended for human consumption purchased from a general meat shop or by mail-order from october 2012 to december 2013. the bacteria were isolated from 105 of the 112 samples (93.8%). prominent differences in the isolation rate due to the place of manufacture/sale or by the cut of the meat were not observed. moreover, in a comparison between domestic (92.6%) and imported (100%) sam ... | 2014 | 25744214 |
| culture-independent identification of bacteria associated with ovine 'broken mouth' periodontitis. | 'broken mouth' periodontitis (bmp) is a painful condition of sheep grazed on rough pasture and involves periodontal infection of the incisor teeth and progressive tooth loss. this can reduce the efficiency of grazing of sheep, which contributes to malnutrition, weight loss, systemic health problems, poor quality of life and early culling from flocks. consequently, this condition is a major economic problem to sheep farmers. however, there are no treatment or control methods available. the aim of ... | 2013 | 23928119 |
| microbiology and foodborne pathogens in honey. | honey has been considered a relatively safe foodstuff due to its compositional properties, with infant botulism caused by clostridium botulinum being the most prominent health risk associated with it. our review is focused on the honey microflora along the food chain and evaluates the pathogenic potential of those microorganisms found in honey. this product may contain a great variety of bacteria and, particularly, fungi that eventually entered the food chain at an early stage (e.g., via pollen) ... | 2017 | 26176586 |
| fractionation and analysis of lipopolysaccharide-derived oligosaccharides by zwitterionic-type hydrophilic interaction liquid chromatography coupled with electrospray ionisation mass spectrometry. | lipopolysaccharide (lps, endotoxin) is a main surface antigen and virulence factor of gram-negative bacteria. regardless of the source of lps, this molecule, isolated from the smooth forms of bacteria, is characterised by a general structural layout encompassing three regions: (i) an o-specific polysaccharide (o-ps) - a polymer of repeating oligosaccharide units, (ii) core oligosaccharide (os), and (iii) the lipid a anchoring lps in the outer membrane of the cell envelope of gram-negative bacter ... | 2016 | 27085741 |
| screening for fecal carriage of mcr-producing enterobacteriaceae in healthy humans and primary care patients. | the extent of the occurrence of the plasmid-encoded colistin resistance genes mcr-1 and mcr-2 among humans is currently sparsely studied in western europe. | 2017 | 28316780 |
| a broadband capacitive sensing method for label-free bacterial lps detection. | in this paper, the authors present a new type of highly sensitive label-free microwave sensor in a form of interdigital capacitor coated with t4 bacteriophage gp37 adhesin. the adhesin binds escherichia coli b (e. coli b) by precise recognizing its bacterial host lipopolysaccharide (lps). the c-terminal part of the adhesin consists of the receptor-binding amino acid residues which are involved in a specific interaction with two terminal glucose residues of the bacterial lps. the change of the se ... | 2016 | 26339930 |
| purification, biochemical characterization, and genetic cloning of the phytase produced by burkholderia sp. strain a13. | a phytase-producing bacterium, burkholderia sp. a13 (jcm 30421), was isolated from lake kasumigaura by enrichment cultivation using minimum medium containing phytic acid as the sole phosphorus source. the phytase production by strain a13 was induced by the presence of phytic acid and repressed by the addition of glucose. the purified enzyme had a molecular weight of 44 kda and a phytase activity of 174 μmol min(-1) mg(-1). the enzyme showed broad substrate specificity, but the highest activity w ... | 2015 | 25833676 |
| human norovirus binding to select bacteria representative of the human gut microbiota. | recent reports describe the ability of select bacterial strains to bind human norovirus, although the specificity of such interactions is unknown. the purpose of this work was to determine if a select group of bacterial species representative of human gut microbiota bind to human norovirus, and if so, to characterize the intensity and location of that binding. the bacteria screened included naturally occurring strains isolated from human stool (klebsiella spp., citrobacter spp., bacillus spp., e ... | 2017 | 28257478 |
| control of shigatoxin-producing escherichia coli in cheese by dairy bacterial strains. | bio-preservation could be a valuable way to control shigatoxin-producing escherichia coli (stec) in cheese. to this end, 41 strains were screened for their inhibitory potential on model cheese curd and on pasteurized and raw milk uncooked pressed cheeses. strains of lactococcus lactis, lactococcus garvieae, leuconostoc pseudomesenteroides, leuconostoc citreum, lactobacillus sp, carnobacterium mobile, enterococcus faecalis, enterococcus faecium, macrococcus caseolyticus and hafnia alvei reduced s ... | 2016 | 26678131 |
| group ii intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer hafnia alvei ad27. | our previous studies showed that glycerol fermentation by hafnia alvei ad27 strain was accompanied by formation of high quantities of lactate. the ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. group ii intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of h. alvei ad27 strain was conducted. the effect of the δldh genotype in h. alvei ad27 strain varied depending on the culture medium a ... | 2017 | 28284024 |
| identification and growth dynamics of meat spoilage microorganisms in modified atmosphere packaged poultry meat by maldi-tof ms. | modified atmosphere packaging (map) is widely used in food industry to extend the microbiological shelf-life of meat. typically, poultry meat has been packaged in a co2/n2 atmosphere (with residual low o2). recently, some producers use high o2 map for poultry meat to empirically reach comparable shelf lifes. in this work, we compared spoilage microbiota of skinless chicken breast in high (80% o2, 20% co2) and low o2 map (65% n2 and 35% co2). two batches of meat were incubated in each atmosphere ... | 2016 | 27554149 |
| mycobacterial antigen 85 complex (ag85) as a target for ficolins and mannose-binding lectin. | the pattern recognition molecules (prms) able to activate complement via the lectin pathway are suspected to be involved in the interaction between pathogenic mycobacteria and the host immune response. recently, we have found strong interactions between 25 and 35kda mycobacterial cell fractions and mannose-binding lectin (mbl) and ficolins. here we demonstrate that two biologically important mycobacterial structures, mannosylated lipoarabinomannan (manlam) and the antigen 85 (ag85) complex, indu ... | 2016 | 27141819 |
| microbiota and metabolome of un-started and started greek-type fermentation of bella di cerignola table olives. | this study aimed to utilize an "omics" approach to evaluate the ability of selected lactobacilli and yeasts to improve the fermentation process of bella di cerignola table olives. four types of fermentations were performed at the pilot-plant scale: un-started fermented olives used as a control (ctrl); olives started with a commercial lactobacillus plantarum strain (s); commercial l. plantarum strain and autochthonous yeast wickeramomyces anomalus disspa73 (sy); and l. plantarum, w. anomalus diss ... | 2015 | 26338113 |
| hafnia psychrotolerans sp. nov., isolated from lake water. | a psychrotolerant, gram-stain-negative, motile, aerobic, peritrichous bacterium, strain djc1-1(t), was isolated from lake dajiaco, tibetan plateau, china. the strain was negative for citrate utilization, lipase activity and α-glucosidase, but positive for the voges-proskauer reaction and n-acetyl-β-glucosaminidase. 16s rrna gene sequence analysis indicated that hafnia paralvei atcc 29927(t), hafnia alvei atcc 13337(t), serratia grimesii dsm 30063(t) and serratia plymuthica dsm 4540(t) were the c ... | 2015 | 25563917 |
| occurrence of glycine in the core oligosaccharides of hafnia alvei lipopolysaccharides--identification of disubstituted glycoform. | endotoxins (lipopolysaccharides, lps) are the main surface antigens and virulence factors of gram-negative bacteria involved for example in the development of nosocomial infections and sepsis. they consist of three main regions: o-specific polysaccharide, core oligosaccharide, and lipid a. bacteria modify lps structure to escape the immune defence, but also to adapt to environmental conditions. lps's structures are highly diversified in the o-specific polysaccharide region to evade bactericidal ... | 2015 | 25541016 |
| a methodological approach to screen diverse cheese-related bacteria for their ability to produce aroma compounds. | microorganisms play an important role in the development of cheese flavor. the aim of this study was to develop an approach to facilitate screening of various cheese-related bacteria for their ability to produce aroma compounds. we combined i) curd-based slurry medium incubated under conditions mimicking cheese manufacturing and ripening, ii) powerful method of extraction of volatiles, headspace trap, coupled to gas chromatography-mass spectrometry (hs-trap-gc-ms), and iii) metabolomics-based me ... | 2015 | 25475278 |
| ficolin-3 activity towards the opportunistic pathogen, hafnia alvei. | ficolin-3 (also called h-ficolin or hakata antigen) is a complement-activating pattern recognition molecule, possessing a fibrinogen-like domain involved in carbohydrate binding. amongst human ficolins, ficolin-3 has the highest concentration in serum and is the most potent lectin pathway activator in vitro. evidence for its physiological function is sparse, although its deficiency has been suggested to increase susceptibility to infections. the specificity of ficolin-3 is poorly characterized a ... | 2015 | 25178935 |
| recovery of hafnia alvei from diseased brown trout, salmo trutta l., and healthy noble crayfish, astacus astacus (l.), in bulgaria. | hafnia alvei was isolated in bulgaria from healthy noble crayfish, astacus astacus (l.), and then from farmed diseased brown trout, salmo trutta l., with signs of haemorrhagic septicaemia. the isolates were identified initially with conventional phenotyping and commercial merlin micronaut and api 20e rapid identification systems, followed by sequencing of the 16s rrna gene. hafnia alvei bt1, bt2 and aa4 were of low virulence to rainbow trout and brown trout, although cytotoxicity was demonstrate ... | 2014 | 24422558 |
| role of 30 kda antigen of enteric bacterial pathogens as a possible arthritogenic factor in post-dysenteric reactive arthritis. | reactive arthritis (rea)/reiter's syndrome (rs) may be caused as a sequel of infections caused by enteric bacterial pathogens, although the mechanisms through, which different pathogens cause similar disease are not clear. | 2013 | 24152499 |
| the italian hafnia alvei strain lmg 27376 is hafnia paralvei. | | 2013 | 24051127 |
| heat resistance of histamine-producing bacteria in irradiated tuna loins. | consumption of foods high in biogenic amines leads to an illness known as histamine, or scombrotoxin, poisoning. the illness is commonly associated with consumption of fish with high levels of histamine ( $ 500 ppm). the objective of this study was to determine and compare the heat resistance of five histamine-producing bacteria in irradiated albacore tuna loins. heat-resistance parameters (d- and z-values) were determined for morganella morganii, raoultella planticola, hafnia alvei, and enterob ... | 2013 | 23992506 |
| reprint of "new complete structure of hafnia alvei clinical isolate strain pcm 2670 semi-rough lipopolysaccharide". | hafnia alvei strain pcm 2670 is a clinical isolate from a patient with chronic reproductive tract infection. the novel structure of the semi-rough lipopolysaccharide was established with the use of nmr spectroscopy and mass spectrometry as well as immunochemical techniques. according to the mass spectrometry data, heptose in the oligosaccharide is partially substituted by glycine. h. alvei pcm 2670 core structure encompasses the common core of h. alvei which is modified with two additional galac ... | 2013 | 23927934 |
| bacterial microbiota of the ocular surface of captive and free-ranging microbats: desmodus rotundus, diameus youngi and artibeus lituratus. | to investigate normal aerobic bacterial microbiota of the eye surface in vvcaptive and free-ranging bats belonging to the suborder microchiroptera. | 2014 | 23710696 |
| new complete structure of hafnia alvei clinical isolate strain pcm 2670 semi-rough lipopolysaccharide. | hafnia alvei strain pcm 2670 is a clinical isolate from a patient with chronic reproductive tract infection. the novel structure of the semi-rough lipopolysaccharide was established with the use of nmr spectroscopy and mass spectrometry as well as immunochemical techniques. according to the mass spectrometry data, heptose in the oligosaccharide is partially substituted by glycine. h. alvei pcm 2670 core structure encompasses the common core of h. alvei which is modified with two additional galac ... | 2013 | 23643833 |