microbial interactions within a cheese microbial community.the interactions that occur during the ripening of smear cheeses are not well understood. yeast-yeast interactions and yeast-bacterium interactions were investigated within a microbial community composed of three yeasts and six bacteria found in cheese. the growth dynamics of this community was precisely described during the ripening of a model cheese, and the lotka-volterra model was used to evaluate species interactions. subsequently, the effects on ecosystem functioning of yeast omissions in ...200817981942
concise chemical synthesis of a tetrasaccharide repeating unit of the o-antigen of hafnia alvei 10457.concise chemical synthesis of a tetrasaccharide repeating unit of the o-antigen of hafnia alvei 10457 is reported. construction of the tetrasaccharide as its 4-methoxyphenyl glycoside was achieved by condensation of less abundant monosaccharide units such as, d-galactofuranose, n-acetyl-d-galactosamine and n-acetylneuraminic acid. the synthetic strategy consists of the preparation of suitably protected required monosaccharide intermediates from the commercially available reducing sugars and high ...200818074224
influence of environmental conditions on biofilm formation by hafnia alvei strains.hafnia alvei is considered an opportunistic pathogen of animal and humans, affecting a wide range of homeothermic and poikilothermic hosts with different body temperatures. in this work, h. alvei strains isolated from different sources were studied with regard to their capacity to form biofilms under different environmental conditions. strain, growth phase, temperature and culture media dependent changes of biofilm formation were semiquantitatively monitored using a microtiter plate method. our ...200818093756
method for reliable isolation of lactobacillus sakei strains originating from tunisian seafood and meat tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. we isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of tunisia, and selectively searched for lactobacillus sakei, a lactic ...200818155310
antibiotic resistance and adherence properties of hafnia alvei clinical isolates: a 19-month study in the hospital of orléans, france.we studied the epidemiology of antibiotic resistance and adherence properties among all hafnia alvei clinical isolates collected from august 2003 to february 2005 from patients hospitalized in the hospital of orléans, france. the isolates were typed by random amplified polymorphic dna (rapd), screened for antibiotic resistance and bacterial adherence to a549 respiratory and t24 bladder cells. six intestinal, 3 respiratory, and 8 isolates from different body sites were collected. a total of 12 ra ...200718230550
lactoferrin-monophosphoryl lipid a complex enhances immunity of mice to plesiomonas shigelloides cnctc 138/92.our previous study showed the efficacy of lactoferrin-monophosphoryl lipid a isolated from hafnia alvei lps complex (lf-mpl(h.a.)) as an adjuvant in stimulation of humoral and cellular immune response in mice to conventional antigens and a lower pyrogenicity of the complex as compared with mpl(h.a.) alone. in the present investigation we demonstrated that lf-mpl(h.a.) complex enhanced the immunity of balb/c mice immunized with plesiomonas shigelloides cnctc 138/92 bacterial vaccine, against p. s ...200818231655
[gene cloning, expression and characterization of a novel phytase from hafnia alvei].a gene appa encoding a novel phytase was firstly cloned from hafnia alvei by pcr and sequenced. the gene was consisted of 1335 bp, encoding 444 amino acids. the calculated molecular weight of the mature appa was about 45.2 kd. the gene appa was expressed in e. coli bl21 (de3). recombinant appa was purified and its enzymatic properties were determined. the optimum ph for the enzyme was 4.5 and the optimum temperature was 60 degrees c. the ph stability of r-appa is good, the relative phytase activ ...200718257229
the gut-brain barrier in major depression: intestinal mucosal dysfunction with an increased translocation of lps from gram negative enterobacteria (leaky gut) plays a role in the inflammatory pathophysiology of depression.there is now evidence that major depression (mdd) is accompanied by an activation of the inflammatory response system (irs) and that pro-inflammatory cytokines and lipopolysacharide (lps) may induce depressive symptoms. the aim of the present study was to examine whether an increased gastrointestinal permeability with an increased translocation of lps from gram negative bacteria may play a role in the pathophysiology of mdd. toward this end, the present study examines the serum concentrations of ...200818283240
damage threshold prediction of hafnia-silica multilayer coatings by nondestructive evaluation of fluence-limiting defects.a variety of microscopic techniques were employed to characterize fluence-limiting defects in hafnia-silica multilayer coatings manufactured for the national ignition facility, a fusion laser with a wavelength of 1.053 mum and a pulse width of 3 ns. photothermal microscopy, with the surface thermal lens effect, was used to map the absorption and thermal characteristics of 3 mm x 3 mm areas of the coatings. high-resolution subaperture scans, with a 1-mum step size and a 3-mum pump-beam diameter, ...200118357189
characterisation of sol-gel prepared (hfo2)x(sio2)1-x (x=0.1, 0.2 and 0.4) by 1h, 13c, 17 o and 29si mas nmr, ftir and tga.the hfo2-sio2 system is attracting interest as a possible new dielectric material in semiconductor devices. knowledge of the location of hafnium within the silica network and the effect hafnium has on the structure will be central to the successful use of this material system in this application. here, sol-gel techniques have been used to manufacture (hfo2)x(sio2)1-x samples (x=0.1, 0.2 and 0.4, each heat treated at 250, 500 and 750 degrees c) and these have been characterised by magic angle spi ...200818373971
laser damage resistance of hafnia thin films deposited by electron beam deposition, reactive low voltage ion plating, and dual ion beam sputtering.a comparative study is made of the laser damage resistance of hafnia coatings deposited on fused silica substrates with different technologies: electron beam deposition (from hf or hfo(2) starting material), reactive low voltage ion plating, and dual ion beam sputtering. the laser damage thresholds of these coatings are determined at 1064 and 355 nm using a nanosecond pulsed yag laser and a one-on-one test procedure. the results are associated with a complete characterization of the samples: ref ...200818449230
identification of a non-pathogenic surrogate organism for chlorine dioxide (clo2) gas treatment.the identification of non-pathogenic surrogate microorganisms is beneficial for determining and validating the efficacy of antimicrobial treatments in food manufacturing environments. a surrogate organism was identified to aid in the decontamination process of fresh produce when treated with chlorine dioxide (clo(2)) gas. thirty-two known strains of pathogenic and non-pathogenic microorganisms and seven unknown microbial isolates from mushroom, tomatoes, and strawberries were evaluated. the prim ...200818456115
enterobacteriaceae and related organisms isolated from shell eggs collected during commercial the united states, commercial shell eggs are washed and graded before retail. since passage of the egg products inspection act in 1971, processing guidelines have been set to ensure that external and internal characteristics are maintained. however, less is known about how commercial processing affects the safety of shell eggs. to identify enteric bacteria entering plants and persisting throughout processing, eggs were collected from 3 us commercial shell egg-processing plants on 3 separate v ...200818493013
vitek 2 failure in screening hafnia alvei inducible beta-lactam resistance. 200818511150
molecular characterization of a cryptic plasmid from the psychrotrophic antarctic bacterium pseudoalteromonas sp. 643a.we report the identification and nucleotide sequence analysis of pkw1, a plasmid of the psychrotrophic bacterium pseudoalteromonas sp. 643a isolated from the stomach of antarctic krill euphasia superba. pkw1 consists of 4583 bp, has a g+c content of 43% and seven putative open reading frames (orfs). the deduced amino acid sequence from orf-1 shared significant similarity with the plasmid replicase protein of psychrobacter cryohalolentis, strain k5. the dna region immediately downstream of the or ...200818611409
thermodynamic investigation of the redox properties for ceria-hafnia, ceria-terbia, and ceria-praseodymia solid solutions.ceria-hafnia, ceria-terbia, and ceria-praseodymia solid solutions were prepared by the citric-acid (pechini) method and characterized using x-ray diffraction (xrd) for structure and coulometric titration for redox thermodynamics. following calcination at 973 k, xrd showed that both ce(0.5)hf(0.5)o2 and ce(0.8)hf(0.2)o2 exist as single-phase solid solutions and that the lattice parameters of the cubic fluorite structures shifted linearly with hf content. following calcination at 1323 k, ce(0.8)hf ...200818630948
an unexpected isolate of hafnia alvei with reduced susceptibility to cefoxitin. 200818653239
[sepsis induced by hafnia alvei in a kidney transplant patient]. 200818662166
[intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees].dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ...200818683661
re: hafnia alvei infection of the deep palmar space. 200818687848
biodiversity of the microbial community in a spanish farmhouse cheese as revealed by culture-dependent and culture-independent methods.the microbial diversity within alberquilla cheese, made from a spontaneously fermented mixture of raw goats' and sheep's milk in the alpujarra mountains (granada, south-east spain), has been studied by the classical culturing method and also by molecular analysis of community dna. a collection of 206 isolates was obtained from the cheese on different selective/differential media, which were then re-grouped to 52 after randomly amplified polymorphic dna (rapd)-pcr analyses. isolates on man-rogosa ...200818692931
graft versus host disease-related hafnia alvei colonization and probable infection.we describe the case of a graft versus host disease (gvhd) patient, in whom hafnia alvei was cultured as a single organism, and at high bacterial counts from stool samples, from the onset of the disease until its resolution. this case is a further example of the contentious role of this species in causing human intestinal disease. furthermore, it focuses on enteric damage by gvhd as a risk factor for acquiring h. alvei colonization, and probably infection.200818719190
diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots.forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ...200818759227
impact on disinfection efficiency of cell load and of planktonic/adherent/detached state: case of hafnia alvei inactivation by plasma activated water.this paper describes the effects of initial microbial concentration and planktonic/adherent/detached states on the efficiency of plasma-activated water. this disinfecting solution was obtained by treating distilled water with an atmospheric pressure plasma produced by gliding electric discharges in humid air. the inactivation kinetics of planktonic cells of hafnia alvei (selected as a bacterial model) were found to be of the first order. they were influenced by the initial microbial concentratio ...200818769918
invasion and intracellular survival of hafnia alvei strains in human epithelial cells.the aim of this study was to investigate the invasion and intracellular survival of different hafnia alvei strains in hela cells.200818795976
structure of the glycerol phosphate-containing o-specific polysaccharide and serological studies on the lipopolysaccharides of citrobacter werkmanii pcm 1548 and pcm 1549 (serogroup o14).the o-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of citrobacter werkmanii pcm 1548 and pcm 1549 (serogroup o14) and found to contain d-glucose, d-glucosamine and glycerol-1-phosphate in molar ratios 2 : 2 : 1. based on methylation analysis and 1h and 13c nuclear magnetic resonance spectroscopy data, it was established that the o-specific polysaccharides from both strains have the identical branched tetrasaccharide repeating unit with 3,6-disubstituted ...200818811720
immunochemical studies of the lipopolysaccharides of hafnia alvei pcm 1219 and other strains with the o-antigens containing d-glucose 1-phosphate and 2-deoxy-2-[(r)-3-hydroxybutyramido]-d-glucose.hafnia alveiis the only species of the genus hafnia, which belongs to the family of enterobacteriaceae. these gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. their lipopolysaccharides (lpss) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. so far, 29 different o-polysaccharide (ops, o-antigen) structures in hafni ...200818836891
[the analysis of the bacterial and fungal flora in maxillary sinuses in patients operated due to fess method].the aim of this work was to analyse the bacterial and fungal flora in maxillary sinuses in patients operated due to the fess method.200818837224
multiscale analysis of the laser-induced damage threshold in optical coatings.we have investigated the influence of laser beam size on laser-induced damage threshold (lidt) in the case of single- and multiple-shot irradiation. the study was performed on hafnia thin films deposited with various technologies (evaporation, sputtering, with or without ion assistance). lidt measurements were carried out at 1064 nm and 12 ns with a spot size ranging from a few tens to a few hundreds of micrometers, in 1-on-1 and r-on-1 modes. these measurements were compared with simulations ob ...200818846165
escherichia albertii and hafnia alvei are candidate enteric pathogens with divergent effects on intercellular tight junctions.attaching-effacing lesion-inducing escherichia albertii and the related, but non-attaching-effacing organism, hafnia alvei, are both implicated as enteric pathogens in humans. however, effects of these bacteria on epithelial cells are not well-characterized. related enteropathogens, including enterohemorrhagic escherichia coli o157:h7, decrease epithelial barrier function by disrupting intercellular tight junctions in polarized epithelia. therefore, this study assessed epithelial barrier functio ...200818930803
two kdo-heptose regions identified in hafnia alvei 32 lipopolysaccharide: the complete core structure and serological screening of different hafnia o serotypes.hafnia alvei, a gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. various 3-deoxy-d-manno-oct-2-ulosonic acid (kdo)-containing fragments different from known structures of core oligosaccharides were previously found among fractions obtained by mild acid hydrolysis of some h. alvei lipopolysaccharides (lpss). however, the positions of these segments in the lps structure were not known. analysis of de- ...200919011031
temperature influences the expression of fimbriae and flagella in hafnia alvei strains: an immunofluorescence study.hafnia alvei, a gram negative bacillus related to the enterobacteriaceae family, is considered an opportunistic pathogen of several animal species and humans. in this communication, we describe fimbrial-like structures from different strains of h. alvei that cannot be easily ascribed to any of the previously reported fimbrial types in this species (type i or type iii). polymerase chain reaction (pcr) and immunofluorescence assays were carried out to study fimbriae and flagella in h. alvei strain ...200919011836
vibrational studies and properties of hybrid inorganic-organic proton conducting membranes based on nafion and hafnium oxide this report, we will describe the effect of different concentrations of hfo2 nanopowders on the structure and properties of [nafion/(hfo2)n] membranes with n = 0, 3, 5, 9, 11, 13, and 15 wt %, respectively. films were prepared by a solvent casting procedure using hfo2 oxoclusters and nafion. seven new homogeneous membranes were obtained with thicknesses ranging from 200 to 350 microm. each membrane is characterized by a rough hfo2-rich surface and a smooth nafion-rich surface, with different ...200819032059
enrichment/isolation of phosphorylated peptides on hafnium oxide prior to mass spectrometric analysis.hafnium oxide (hafnia) exhibits unique enrichment properties towards phosphorylated peptides that are complementary to those of titanium oxide (titania) and zirconium oxide (zirconia) for use with mass spectrometric analysis in the field of proteomics.200919082170
normalization of leaky gut in chronic fatigue syndrome (cfs) is accompanied by a clinical improvement: effects of age, duration of illness and the translocation of lps from gram-negative bacteria.there is now evidence that an increased translocation of lps from gram negative bacteria with subsequent gut-derived inflammation, i.e. induction of systemic inflammation and oxidative & nitrosative stress (io&ns), is a new pathway in chronic fatigue syndrome (cfs).200819112401
isolation of colistin-resistant hafnia alvei. 200919141752
microbial inactivation using plasma-activated water obtained by gliding electric evaluate the microbial disinfection efficacy of a plasmachemical solution obtained by the activation of water with gliding electric discharges.200919170858
molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk.the aim of this study was to use molecular techniques to assess the microbiota of eight raw cow's milk samples at biotype and species level. sixty-six isolates from raw milk samples were screened by randomly amplified polymorphic dna-pcr (rapd-pcr) biotyping and representative strains of rapd-pcr profiles were identified by 16s rrna gene sequencing. pseudomonas spp. were the most commonly occurring contaminants along with enterobacteriaceae such as hafnia alvei, serratia marcescens and citrobact ...200919171267
fourier transform infrared spectroscopy and solid-state nuclear magnetic resonance studies of octadecyl modified metal oxides obtained from different silane precursors.octadecyl (c(18)) modified metal oxide substrates, including titania, zirconia, hafnia, and alumina, are prepared using two types of silylating reagents, n-octadecyltrihydridosilane and n-octadecyltrichlorosilane. fourier transform infrared (ftir) and solid-state (29)si nuclear magnetic resonance (nmr) measurements are performed to examine the cross-linking of the silanes. solid-state (13)c nmr spectroscopy provides information about the conformation and mobility of surface-immobilized alkyl cha ...200919178911
isolation of hafnia species from kimchi.eight commercially packaged kimchi products were examined over 15 days of storage at 4 degrees to evaluate the occurrence of hafnia alvei (h. alvei). additionally, 7 saeujeot products, as a possible ingredient source, were analyzed to examine the bacteria's origin. over the storage period, kimchi samples had decreasing ph levels, which stabilized at ph 4.2; acidity increased to 0.9+/-0.1%. lactose-nonfermenting bacteria, which h. alvei belongs to, gradually reduced in numbers over the kimchi sto ...200919190412
[taxonomic characteristics and physiological properties of microorganisms from the gut of pike (esox lucius)].the taxonomic composition and distribution of microorganisms differing in the degree of association with the intestinal mucosa of the pike (lucius lucius) has been studied. microorgansism of the families enterobacteriaceae, aeromonadaceae, and vibrionaceae dominate in the gut microflora. numerically prevailing bacterial species are characterized by high proteolytic and amylolytic enzyme activities as well as by high persistence accounted for by antilysozyme and antihistone activities. the result ...200819198074
comparison of pcr-based molecular markers for the characterization of proteus mirabilis clinical isolates.proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. for epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (pfge) or ribotyping, have been developed for this pathogen. however, these methods are labor intensive a ...200819219283
reactive landing of gas-phase ions as a tool for the fabrication of metal oxide surfaces for in situ phosphopeptide enrichment.zirconium, titanium, and hafnium oxide-coated stainless steel surfaces are fabricated by reactive landing of gas-phase ions produced by electrospray ionization of group ivb metal alkoxides. the surfaces are used for in situ enrichment of phosphopeptides before analysis by matrix-assisted laser desorption ionization (maldi) mass spectrometry. to evaluate this method we characterized zro(2) (zirconia) surfaces by (1) comparison with the other group ivb metal oxides of tio(2) (titania) and hfo(2) ( ...200919251440
polyphasic study of microbial communities of two spanish farmhouse goats' milk cheeses from sierra de aracena.the microbial communities present in 2 different types of farmhouse goats' milk cheese from the aracena mountains (southwest spain), quesailla arochena (hard cheese) and torta arochena (soft cheese), have been studied using both culture-dependent and culture-independent techniques. all bacterial isolates were clustered by using randomly amplified polymorphic dna (rapd) and identified by 16s rrna gene sequencing, species-specific pcr and multiplex pcr. thus a total of 26 different species were id ...200919269572
targeted capture of pathogenic bacteria using a mammalian cell receptor coupled with dielectrophoresis on a biochip.efficient capture of target analyte on biosensor platforms is a prerequisite for reliable and specific detection of pathogenic microorganisms in a microfluidic chip. antibodies have been widely used as ligands; however, because of their occasional unsatisfactory performance, a search for alternative receptors is underway. heat shock protein 60 (hsp60), a eukaryotic mitochondrial chaperon protein is a receptor for listeria adhesion protein (lap) during listeria monocytogenes infection. this paper ...200919317455
molecular characterisation of the reca locus in clinical isolates of verocytotoxigenic e. coli o157:h7.molecular epidemiology of verocytoxigenic escherichia coli o157:h7 is important to help elucidate reservoirs and modes of transmission, particularly between animals and humans. as the reca gene locus is now beginning to gain application in bacterial genotyping schemes, and as it has not been examined previously in e. coli o157 isolates, this study aims to examine potential polymorphic variation as a possible epidemiological marker for the subspecies characterisation of clinically significant ver ...200919348125
[aberrant gallstones--extraordinary complications of laparoscopic cholecystectomy].about 0.2 % of patients undergoing laparoscopic cholecystetomy will suffer from complications caused by lost gallstones. diagnostic and therapeutic measures are correlated to the symptoms. at different locations, abscesses can arise, which can be treated by direct access through the -abdominal wall, laparotomy or laparoscopy. two cases are presented with the especially grave sequels of subphrenic abscess, infiltration of the thoracic wall, and pleural empyema, which -respectively needed several ...200919382051
clostridium perfringens liver abscess after pancreatic resection.liver abscess is a rare complication after pancreatectomy. clostridium perfingens is a rare cause of intrahepatic infections.200919388837
effects of proteus vulgaris growth on the establishment of a cheese microbial community and on the production of volatile aroma compounds in a model investigate the impact of proteus vulgaris growth on a multispecies ecosystem and on volatile aroma compound production during cheese ripening.200919426267
evaluation of the anti-inflammatory activity of biolysat hafnia(r), in vivo in man.synopsis 'biolysat hafnia(r)', an active principle, whose anti-inflammatory activity had been demonstrated in animals, also has anti-inflammatory properties in vivo in human volunteers as shown by the technique of migration chambers after suction blisters. evaluation de l'activité anti-inflammatoire du biolysat hafnia(r), en vivo chez l'homme.198519460021
gangrenous cholecystitis caused by hafnia alvei: a case report and review of literature. 200919497550
phenotypic characterization of human pathogenic bacteria in fish from the coastal waters of south west cameroon: public health implications.increasing economic and recreational opportunities, attractive scenery and a perception of a better quality of life are luring people to the coast. unfortunately, these activities together with the commensurate increase in population in the area inevitably result in pollution of coastal waters with excessive microorganisms and other pollutants. microbial pollutants not only contaminate the coastal water but also aquatic food sources, thus posing a health risk to consumers. fish is a major source ...200919658320
gram-negative bacteria associated with brewery yeasts: reclassification of obesumbacterium proteus biogroup 2 as shimwellia pseudoproteus gen. nov., sp. nov., and transfer of escherichia blattae to shimwellia blattae comb. nov.phylogenetic analyses of type and reference strains of obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16s rrna gene sequences and partial sequences of four protein-coding genes (fusa, leus, pyrg and rpob). both approaches revealed that o. proteus biogroup 1 strains were closely related to hafnia alvei. biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included escherichia blattae. phenotypic tests were ...201019661513
structural analysis of the lipid a isolated from hafnia alvei 32 and pcm 1192 lipopolysaccharides.hafnia alvei, a gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. the majority of clinical symptoms of diseases caused by this bacterium have a lipopolysaccharide (lps, endotoxin)-related origin. the lipid a structure affects the biological activity of endotoxins predominantly. thus, the structure of h. alvei lipid a was analyzed for the first time. the major form, asymmetrically hexa-acylated lipid ...201019706748
hafnia paralvei sp. nov., formerly known as hafnia alvei hybridization group has been shown previously, based largely on dna-dna hybridizations and partial 16s rrna gene sequencing, that hafnia alvei is genotypically heterogeneous and consists of at least two dna hybridization groups (hgs). in the present study, the taxonomic status of h. alvei hgs 1 and 2 was reassessed. a panel of 24 reference strains and isolates previously assigned to one of the two hgs in h. alvei was subjected to (gtg)5-pcr fingerprinting; this resulted in the delineation of two (gtg)5-pcr clust ...201019734282
enterobacteriaceae and related organisms isolated from nest run cart shelves in commercial shell egg processing facilities.enterobacteriaceae, including salmonella, may be recovered from foods and processing facilities. high levels of enterobacteriaceae in the processing plant environment can be an indication of inadequate sanitation. this experiment was designed to determine if nest run egg carts serve as reservoirs for enterobacteriaceae. eggs that are produced by hens not housed in buildings connected to the processing plant are referred to as nest run. many of these eggs are transported to the plant on carts to ...200919762864
[hafnia alvei]. 200919802404
inhibition of fungi and gram-negative bacteria by bacteriocin bactn635 produced by lactobacillus plantarum sp. tn635.the aim of this study was to evaluate 54 lactic acid bacteria (lab) strains isolated from meat, fermented vegetables and dairy products for their capacity to produce antimicrobial activities against several bacteria and fungi. the strain designed tn635 has been selected for advanced studies. the supernatant culture of this strain inhibits the growth of all tested pathogenic including the four gram-negative bacteria (salmonella enterica atcc43972, pseudomonas aeruginosa atcc 49189, hafnia sp. and ...201019888697
technology-induced selection towards the spoilage microbiota of artisan-type cooked ham packed under modified atmosphere.the microbiota associated with a highly-perishable belgian artisan-type cooked ham was analyzed through plating and (gtg)(5)-fingerprinting of isolates throughout its processing chain. the raw tumbled meat was characterized by the presence of a versatile microbiota around 4.8 log(cfu g(-1)), consisting of lactic acid bacteria, staphylococci, brochothrix thermosphacta, gram-negative bacteria, and yeasts. pasteurisation of the ham logs reduced bacterial counts below 2 log(cfu g(-1)) and subsequent ...201019913696
novel o-antigen of hafnia alvei pcm 1195 lipopolysaccharide with a teichoic acid-like structure.the lipopolysaccharide (lps) of hafnia alvei strain pcm 1195 was obtained by the hot phenol/water method. the o-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. the structure of the o-specific polysaccharide was investigated by (1)h, (13)c, and (31)p nmr spectroscopy, maldi-tof ms, and gc-ms, accompanied by monosaccharide and methylation analysis. it was concluded that the o-specific polysaccharide is composed of a hexasaccharide repeating units inte ...201020003963
inhibition of the early stage of salmonella enterica serovar enteritidis biofilm development on stainless steel by cell-free supernatant of a hafnia alvei culture.compounds present in hafnia alvei cell-free culture supernatant cumulatively negatively influence the early stage of biofilm development by salmonella enterica serovar enteritidis on stainless steel while they also reduce the overall metabolic activity of s. enteritidis planktonic cells. although acylhomoserine lactones (ahls) were detected among these compounds, the use of several synthetic ahls was not able to affect the initial stage of biofilm formation by this pathogen.201020097823
histamine, cadaverine, and putrescine produced in vitro by enterobacteriaceae and pseudomonadaceae isolated from spinach.a total of 364 bacterial isolates, obtained from spinach leaves, were assayed in a decarboxylase broth containing histidine, lysine, and ornithine to check their ability to produce biogenic amines, and then quantified by high-performance liquid chromatography. among these isolates, 240 formed cadaverine, 208 formed putrescine, and 196 formed histamine, in widely varying amounts. they frequently produced more than one biogenic amine. klebsiella pneumoniae subsp. pneumoniae and morganella morganii ...201020132689
biofilm formation and acyl homoserine lactone production in hafnia alvei isolated from raw milk.the objective of this study was to detect the presence of acyl homoserine lactones (ahls), signal molecules of the quorum sensing system in biofilm formed by hafnia alvei strains. it also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. ahls were assayed using well diffusion techniques, thin layer chromatography (tlc) and detection directly in biofilm with biomonitors. the extracts obtained from planktonic and sessile cell of h. alvei induced at least two of thre ...201020140298
[report of four clinical cases of hafnia alvei bacteremia in a pediatric cardiac surgery unit].the gram-negative bacillus hafnia alvei is the only species of the genus hafnia, family enterobacteriaceae. it occasionally behaves as an opportunistic pathogen in humans, causing intestinal and respiratory infection and sepsis. it rarely causes bacteremia, usually of unknown focus.201020140313
an unusual organism for pd-related peritonitis: hafnia alvei. 201020200377
emergence of carbapenem-resistant hafnia: the fall of the last soldier. 201020397936
optical properties of cubic hafnia stabilized with yttria.the composition, transparency, refractive index, and infrared reflectance of yttria-stabilized cubic hafnia (c-hfo(2)) single crystals were measured. the material is transparent from the ultraviolet to the mid-infrared and for 9.6-mol % y(2)o(3), the index is slightly smaller than for comparable cubic zirconia c-zro(2) or for diamond, but the dispersion (n(f) - n(c) = 0.02811) is larger than that of diamond. the index vs wavelength from 0.36 microm in the ultraviolet to 5.0 microm in the infrare ...199020556154
production of biogenic amines "in vitro" in relation to the growth phase by enterobacteriaceae species isolated from traditional sausages.histidine, lysine, ornithine and tyrosine decarboxylase activities were tested in 79 strains of enterobacteriaceae (41 of hafnia alvei, 17 of serratia liquefaciens, 5 of enterobacter cloacae, 4 of citrobacter braakii, 2 of proteus vulgaris, 2 of proteus mirabilis, 2 of providencia stuartii, 2 of klebsiella terrigena, 1 of rahnella aquatilis, 1 of salmonella arizonae, 1 of citrobacter youngae and 1 of escherichia coli) isolated from botillo, a spanish traditional sausage. in general, the strains ...201020673614
testing of the durability of single-crystal calcium fluoride with and without antireflection coatings for use with high-power krf excimer lasers.the transmission of uncoated and antireflection (ar)-coated caf(2) windows has been measured during long krf laser exposures. samples were tested for up to 75 million pulses in front of an industrial-grade krf excimer laser at high repetition rates (200-300 hz) and moderately high energy fluence (220-550 mj/cm(2)). in this fluence range bare caf(2) performs much better than the best fused silicas currently available. various ar coatings (whose structures were initially unknown to us) were deposi ...199220733809
reactive evaporation of low-defect density hafnia.motivation for this work includes observations at lawrence livermore national laboratory of a correlation between laser damage thresholds and both the absorption and the nodular-defect density of coatings. activated oxygen is used to increase the metal-oxidation kinetics at the coated surface during electron-beam deposition. a series of hafnia layers are made with various conditions: two µ-wave configuations, two sources (hafnium and hafnia), and two reactive oxygen pressures. laser damage thres ...199320856370
selective testing of ß-galactosidase activity in the laboratory identification of salmonella and shigella species.salmonella and shigella species are pathogens of great medical and public health importance. however, laboratory identification of these organisms is time consuming. using current standard laboratory algorithms, the vast majority of organisms submitted for serological typing with salmonella-specific and shigella-specific antisera are not clinically significant.201020924038
solvothermal and surfactant-free synthesis of crystalline nb(2)o(5), ta(2)o(5), hfo(2), and co-doped hfo(2) nanoparticles.a simple route to niobium, hafnium and tantalum oxide nanocrystals using a nonaqueous sol-gel route based on the solvothermal reaction of the corresponding metal chlorides with benzyl alcohol is presented. this approach can easily be extended to the preparation of high quality co-doped hfo(2) nanoparticles of uniform size and shape and with a homogenous distribution of the magnetic ions. the structural characterization of all these nanomaterials as well as the magnetic properties of pure and dop ...201020976360
improved selective and differential medium for isolation of escherichia coli o157:h7.gmac, a modified version of sorbitol macconkey medium (smac), was produced with a reduced quantity of selective agents and incorporated gentiobiose. gmac supported a higher recovery rate of heat- or acid-injured escherichia coli o157:h7 cells than smac with cefixime and tellurite (ct-smac), while differentiating e. coli o157:h7 from sorbitol-nonfermenting hafnia alvei.201020980579
optical properties of hafnia and coevaporated hafnia:magnesium fluoride thin films.mixed films of hfo(2) and mgf(2) were prepared by the e-beam coevaporation of two source materials with or without the presence of ion assistance. optical properties and hardness of mixed films were compared among various compositions that were achieved by controlling the deposition rate of mgf(2). results obtained from pure materials confirmed the densification of hfo(2) and the increased absorption in mgf(2) with a higher discharge voltage. a refractive index of a composite film from 1.38 to 1 ...199621102941
characterization of the enterobacteriaceae community that developed during storage of minced beef under aerobic or modified atmosphere packaging conditions.the whole cell protein and macrorestriction analysis of dna of enterobacteriaceae isolates recovered from minced beef stored at 0, 5, 10 and 15 °c aerobically and under modified atmosphere packaging consisting of 40% co(2)-30% o(2)-30% n(2) in the presence (map+) and absence (map-) of oregano essential oil were studied. sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) profiles obtained from whole cell protein analysis of the enterobacteriaceae isolates revealed seven groups. ...201021216482
hafnia alvei pyelonephritis in a renal transplant recipient: case report and review of an under-recognized nosocomial pathogen.a.p. cardile, d. forbes, v. cirigliano, b. stout, n.p. das, g. hsue. hafnia alvei pyelonephritis in a renal transplant recipient: case report and review of an under-recognized nosocomial pathogen. transpl infect dis 2011: 13: 407-410. all rights reserved abstract: we describe the first case to our knowledge of hafnia alvei pyelonephritis in a renal transplant recipient. clinicians should consider this under-recognized pathogen when clinically evaluating immunosuppressed patients with a history o ...201121299775
strong influence of polymer architecture on the microstructural evolution of hafnium-alkoxide-modified silazanes upon ceramization.the present study focuses on the synthesis and ceramization of novel hafnium-alkoxide-modified silazanes as well as on their microstructure evolution at high temperatures. the synthesis of hafnia-modified polymer-derived sicn ceramic nanocomposites is performed via chemical modification of a polysilazane and of a cyclotrisilazane, followed by cross-linking and pyrolysis in argon atmosphere. spectroscopic investigation (i.e., nmr, ftir, and raman) shows that the hafnium alkoxide reacts with the n ...201121381195
femtosecond pulse damage thresholds of dielectric coatings in vacuum.the dielectric breakdown behavior of dielectric coatings in studied for different ambient gas pressures with femtosecond laser pulses. at 10(-7) torr, the multiple femtosecond pulse damage threshold, fm, is about 10% of the single pulse damage fluence f(1) for hafnia and silica films compared to about 65% and 50%, respectively, at 630 torr. in contrast, the single-pulse damage threshold is pressure independent. the decrease of fm with decreasing air pressure correlates with the water vapor and o ...201121445209
laser-induced damage of hafnia coatings as a function of pulse duration in the femtosecond to nanosecond range.laser-damage thresholds and morphologies of hafnia single layers exposed under femtosecond, picosecond, and nanosecond single pulses (1030/1064 nm) are reported. the samples were made with different deposition parameters in order to study how the damage behavior of the samples evolves with the pulse duration and how it is linked to the deposition process. in the femtosecond to picosecond regime, the scaling law of the laser-induced damage threshold as a function of pulse duration is in good agre ...201121460935
improved scratch resistance for antireflective coatings on sapphire.antireflective coatings on sapphire were optimized by variation of the coating design, the total thickness, and the highly refractive material used. the coatings were characterized with a focus on their scratch resistance. an increased resistance against scratching wear is shown for hafnia-containing coatings with a total thickness of about 500?nm.201121460948
comparisons between laser damage and optical electric field behaviors for hafnia/silica antireflection coatings.we compare designs and laser-induced damage thresholds (lidts) of hafnia/silica antireflection (ar) coatings for 1054?nm or dual 527?nm/1054?nm wavelengths and 0° to 45° angles of incidence (aois). for a 527?nm/1054?nm, 0° aoi ar coating, lidts from three runs arbitrarily selected over three years are ~20?j/cm2 or higher at 1054?nm and <10?j/cm2 at 527?nm. calculated optical electric field intensities within the coating show two intensity peaks for 527?nm but not for 1054?nm, correlating with th ...201121460961
a case of bacteremia caused by hafnia paralvei.the genus hafnia, a member of the enterobacteriaceae, is widespread in nature and rarely causes human infection. we describe a case of an 85-year-old japanese man hospitalized consequent to suspected cholecystitis, in which hafnia sp. was recovered from the blood culture concomitantly with enterococcus faecalis. sequencing of the 16s ribosomal rna gene sequence and phenotyping with id 32 e revealed that the recovered hafnia sp. was considered to be hafnia alvei genomosp. 2 (atcc 29927), recently ...201121553084
clinical and laboratory diagnostic characteristics and cytotoxigenic potential of hafnia alvei and hafnia paralvei strains.a collection of 68 strains of hafnia previously identified to species by 16s rrna gene sequencing were investigated for simple phenotypic properties that could aid in their recognition in the clinical laboratory. four tests including malonate utilization, fermentation of salicin and d-arabinose, and expression of ß-glucosidase activity correctly assigned each strain to either h. alvei or h. paralvei. antibiotic susceptibility profiles for 35 hafnia alvei and hafnia paralvei isolates were generat ...201121795516
ab initio study of the elastic properties of single and polycrystal tio(2), zro(2) and hfo(2) in the cotunnite this work, we study theoretically the elastic properties of the orthorhombic (pnma) high-pressure phase of iv-b group oxides: titania, zirconia and hafnia. by means of the self-consistent siesta code, pseudopotentials, density functional theory in the lda and gga approximations, the total energies, hydrostatic pressures and stress tensor components are calculated. from the stress-strain relationships, in the linear regime, the elastic constants c(ij) are determined. derived elastic constants, ...200921817222
surface microbial consortia from livarot, a french smear-ripened cheese.the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ...201121815832
first-principles study of fission product (xe, cs, sr) incorporation and segregation in alkaline earth metal oxides, hfo(2), and the mgo-hfo(2) order to close the nuclear fuel cycle, advanced concepts for separating out fission products are necessary. one approach is to use a dispersion fuel form in which a fissile core is surrounded by an inert matrix that captures and immobilizes the fission products from the core. if this inert matrix can be easily separated from the fuel, via e.g.-ásolution chemistry, the fission products can be separated from the fissile material. we examine a surrogate dispersion fuel composition, in which hafn ...200921715804
detection of bacteria in normal adult nasal cavity based on polymerase chain reaction-denaturing gradient gel electrophoresis.this study investigated the bacterial diversity in a normal adult nasal cavity using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) of 16s ribosomal rna (rrna) gene fragments and compared the results with those of a culture-based method.201121711966
yokenella regensburgei in an immunocompromised host: a case report and review of the literature.yokenella regensburgei belongs to the enterobacteriaceae and shares some biochemical characteristics with hafnia alvei. a few case reports have suggested that it is an opportunistic pathogen, but there is no strong evidence to support its clinical importance. until recently, it was difficult to accurately differentiate between y. regensburgei and h. alvei by use of routine identification techniques. here, we present a case of soft tissue infection and bacteremia caused by y. regensburgei, which ...201121706224
growth, structure, and thermal conductivity of yttria-stabilized hafnia thin films.yttria-stabilized hafnia (ysh) films of 90 nm thickness have been produced using sputter-deposition by varying the growth temperature (t(s)) from room-temperature (rt) to 400 °c. the effect of t(s) on the structure, morphology, and thermal conductivity of ysh films has been investigated. structural studies indicate that ysh films crystallize in the cubic phase. the lattice constant decreases from 5.15 to 5.10 å with increasing t(s). the average grain size (l) increases with increasing t(s); l-t( ...201122148322
Ecological and aromatic impact of two Gram-negative bacteria (Psychrobacter celer and Hafnia alvei) inoculated as part of the whole microbial community of an experimental smear soft cheese.The impact of the growth of two Gram-negative bacteria, Psychrobacter celer and Hafnia alvei, inoculated at 10(2) and 10(6)cfu/g, on the dynamics of a multispecies community as well as on volatile aroma compound production during cheese ripening was investigated. Results showed that P. celer was able to successfully implant itself in cheese, regardless of its inoculation level. However, when it was inoculated at a high level, the bacterial biodiversity was drastically lowered from day 25 to the ...201122177851
biogenic amines produced by enterobacteriaceae isolated from meat products.biogenic amines in ground meat and processed meat products are one of the indicators to determine the poor quality raw material. major histamine forming bacterium was escherichia coli (strain ec04 with 65.88 mg/100 ml in brain heart infusion medium), followed by the microorganisms morganella morganii (strain mm4 with 8.40 mg/100 ml and strain mm7 with 8.28 mg/100 ml) and proteus mirabilis (strain pm02 with 8.76 mg/100 ml), respectively. the highest putrescine production level was found in citrob ...200122062111
Bacterial infections of Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to gamete collecting weirs in Michigan.Herein, we describe the prevalence of bacterial infections in Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to spawn in two tributaries within the Lake Michigan watershed. Ten bacterial genera, including Renibacterium, Aeromonas, Carnobacterium, Serratia, Proteus, Pseudomonas, Hafnia, Salmonella, Shewanella and Morganella, were detected in the kidneys of Chinook salmon (n = 480) using culture, serological and molecular analyses. Among these, Aeromonas salmonicida was detected at ...201222168454
new functional ligands for ficolin-3 among lipopolysaccharides of hafnia alvei.ficolin-1 (m), ficolin-2 (l), ficolin-3 (h) and mannan-binding lectin (mbl) activate the complement system and have opsonic activity. the specificity of ficolin-3 is poorly characterized and currently limited to a few ligands only. we present new specific targets for human ficolin-3, identified among lipopolysaccharides (lpss, endotoxin) of hafnia alvei. the interaction was restricted to lpss of four strains: 23, polish collection of microorganisms (pcm) 1200, pcm 1203 and pcm 1205 and limited t ...201121890891
quasiparticle excitations and charge transition levels of oxygen vacancies in hafnia.we calculate the quasiparticle defect states and charge transition levels (ctls) of oxygen vacancies in monoclinic hafnia using density functional theory (dft) and the gw method. we introduce the criterion that the quality and reliability of ctls may be evaluated by calculating the same ctl via two physical paths and show that it is necessary to include important electrostatic corrections previously neglected within the supercell dft+gw approach. contrary to previous reports, the oxygen vacancie ...201122181908
identification of the methyl phosphate substituent at the non-reducing terminal mannose residue of the o-specific polysaccharides of klebsiella pneumoniae o3, hafnia alvei pcm 1223 and escherichia coli o9/o9a lps.o-specific polysaccharides of gram-negative bacteria are synthesized by two different mechanisms: polymerization of the pre-formed o-repeating unit or sequential addition of the monosaccharides to the growing polysaccharide chain. in the second case, growth of the polymer can be further subdivided into two groups depending on the presence or absence of a special monosaccharide or non-sugar substituent that terminates the glycan. a family of polymannose o-polysaccharides provides prototypes for t ...201122169179
necrotizing fasciitis following endoscopic harvesting of the greater saphenous vein for coronary artery bypass graft.the greater saphenous vein (gsv) remains the most commonly harvested conduit for revascularization in coronary artery bypass grafting (cabg). published literature shows that minimally invasive vein harvesting techniques have a significantly lower incidence of wound infection rates than conventional open vein harvesting techniques have. we report a case of necrotizing fasciitis, an infection with a mortality rate of 30% to 50%, after endoscopic harvesting of the greater saphenous vein to be used ...201121902951
microbiological characteristics of precooked, vacuum-packaged uncured beef and pork.microbiological examination of 29 packages of precooked, vacuum-packaged beef and pork products purchased at local and regional supermarkets along with 110 packages of precooked, vacuum-packaged, sliced roast beef obtained from a major meat processor did not disclose the presence of escherichia coli, coagulase-positive staphylococcus aureus, clostridium perfringens or salmonella. the effects of storage temperature and length of storage on the microflora of 110 packages of sliced roast beef were ...198922056107
a note on escherichia coli o157:h7 serotype in turkish meat products.255 minced beef, 101 soudjouk and 50 uncooked hamburger samples were analyzed for the presence of escherichia coli o157:h7 serotype. m-ec and lst broths were used as selective enrichment media and smac agar was used as a selective isolation medium. a total of 3 e. coli o157 were isolated by conventional culture techniques; one from each of minced beef, uncooked hamburger and soudjouk but none were identified as the h7 serotype. for determination of selective media-growing cohabitant bacteria, 26 ...200022062162
Identification of Yersinia ruckeri from diseased salmonid fish by Fourier transform infrared spectroscopy.Yersinia ruckeri is the causative agent of enteric redmouth disease (ERM), which mainly affects salmonid fish. Isolates of Y. ruckeri from diseased salmonid fish were obtained over a 6-year period from eight fish farms in the State of Baden-Württemberg, Southwest Germany. The strains were characterized by biochemical methods and Fourier transform infrared spectroscopy (FT-IR) combined with artificial neural network analysis. These methods were complemented by 16S rDNA sequencing for several isol ...201222103737
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