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ft-ir microspectroscopy for early identification of some clinically relevant pathogens.to investigate the potentials and limitations of fourier transform-infrared (ft-ir) microspectroscopy as a tool to identify, at the level of microcolonies, pathogenic bacteria frequently isolated in the clinical environment.200616968290
effects of bifidobacterium lactis bb12 supplementation on intestinal microbiota of preterm infants: a double-blind, placebo-controlled, randomized study.the gastrointestinal microbiota of preterm infants in a neonatal intensive care unit differs from that of term infants. in particular, the colonization of preterm infants by bifidobacteria is delayed. a double-blind, placebo-controlled, randomized clinical study was performed on 69 preterm infants to investigate the role of bifidobacterium lactis bb12 supplementation in modifying the gut microbiota. both culture-dependent and culture-independent approaches were used to study the gut microbiota. ...200616971641
first detection of the ambler class c 1 ampc beta-lactamase in citrobacter freundii by a new, simple double-disk synergy test.we report on the first detection of an ampc-type ambler class c 1 (acc-1) beta-lactamase in citrobacter freundi isolated from a patient also harboring acc-1-producing escherichia coli and klebsiella pneumoniae. we propose a simple cefoxitin-based double-disk synergy test (ddst) for the specific detection of acc-1 in members of the family enterobacteriaceae, including natural ampc producers, in association with a cloxacillin-based ddst as a first-line ampc-type beta-lactamase screening test.200616971653
[pyogenic spondylitis by enterobacter cloacae as a postoperative complication of turp: a case report].we report a case of pyogenic spondylitis caused by enterobacter cloacae as a rare complication of transurethral resection of the prostate (turp). a 79-year-old man underwent turp. immediate after removal of urethral catheter on postoperative day (pod) 7, he developed high fever > 40 degrees c with increased acute inflammatory reaction. urine and blood culture detected e. cloacae and methicillin-resistant staplylococcus aureus. he complained of lumbago since pod 9. two-week administration of imip ...200616972629
changes in the subgingival microbiota and periodontal parameters before and 3 months after bracket placement.dental plaque is organized in a biofilm complex that provides protection and nutrients for periodontopathic bacteria. several factors can affect microbial colonization, including restorations and orthodontic brackets. the aim of this study was to investigate changes in subgingival microbiota and clinical parameters before and after bracket placement.200616979483
in vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections.against 443 aerobic and anaerobic bacteria isolated from diabetic foot infections, ceftobiprole mics (microg/ml) at which 90% of the isolates tested were inhibited were as follows: methicillin-resistant staphylococcus aureus, 1; methicillin-susceptible s. aureus and staphylococcus lugdunensis, 0.5; anaerococcus prevotii, 0.125; finegoldia magna, 0.5; peptoniphilus asaccharolyticus, 1; peptostreptococcus anaerobius, 4; escherichia coli and enterobacter species, 0.125; klebsiella species, 2; and p ...200616982780
influence of culture site-specific mic distributions on the pharmacokinetic and pharmacodynamic properties of piperacillin/tazobactam and piperacillin: a data analysis.investigators who perform pharmacokinetic/pharmacodynamic (pk-pd) modeling with monte carlo simulation have historically not stratified microbiological data by culture site. this lack of stratification might be problematic if susceptibility patterns differ among sites and might lead to differences in pk-pd.200616990081
potential prophylactic value of bovine colostrum in necrotizing enterocolitis in neonates: an in vitro study on bacterial attachment, antibody levels and cytokine production.necrotizing enterocolitis (nec) is an important disease of low birth-weight neonates. the immaturity of the gut mucosa may result in close contact between the host epithelium and microorganisms which are normally confined to the gut lumen. damage of the mucosa due to endotoxin, cytokine production or other factors is believed to then occur. the aim of this study was to determine whether spray-dried bovine colostrum demonstrated potential in vitro as a prophylactic for nec. antiadherence was meas ...200616995878
dissemination of extended-spectrum beta-lactamase-producing enterobacteriaceae in intensive care units of a medical center in taiwan.the prevalence of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in a tertiary hospital in taiwan was assessed over a 16-month period. a total of 125 nonrepetitive esbl-producing isolates of enterobacter cloacae, escherichia coli, and klebsiella pneumoniae were available for investigation using molecular methods. four predominant intensive care units (icus) were identified, and shv-12 (59%), ctx-m- 3 (36%), and ctx-m-14 (14%) were the three most frequent esbls. shv-12 was p ...200617002548
two-center collaborative evaluation of performance of the bd phoenix automated microbiology system for identification and antimicrobial susceptibility testing of gram-negative bacteria.the performance of the bd phoenix automated microbiology system (bd diagnostic systems, sparks, md) was assessed for identification (id) and antimicrobial susceptibility testing (ast) of the majority of clinically encountered bacterial isolates in a european collaborative two-center trial. a total of 494 bacterial isolates including various species of the enterobacteriaceae and 110 nonfermentative gram-negative bacteria were investigated: of these, 385 were single patient isolates, and 109 were ...200617005752
impact of inactive empiric antimicrobial therapy on inpatient mortality and length of stay.the consequences of inactive empiric antimicrobial therapy are not well-described and may cause prolonged hospitalization or infection-related mortality. in vitro susceptibility results for 884 patients hospitalized at an academic medical center with gram-negative bloodstream infections (gnbi) from 2001 to 2003 were matched to antimicrobial orders within 24 h of culture. clinical characteristics, organism, inpatient mortality, and length of stay after culture for patients with gnbi were compared ...200617005817
effect of tigecycline on normal oropharyngeal and intestinal microflora.antibacterial agents disrupt the ecological balance of the normal human microflora. tigecycline, a member of a new class of antibiotics (glycylcyclines), has been shown to have a potent broad-spectrum activity against most gram-positive and gram-negative aerobic and anaerobic bacteria. the aim of the study was to investigate the ecological effects of tigecycline on the normal oropharyngeal and intestinal microflora of healthy subjects. thirteen healthy white subjects (six females and seven males ...200617005820
high-level carbapenem resistance in a klebsiella pneumoniae clinical isolate is due to the combination of bla(act-1) beta-lactamase production, porin ompk35/36 insertional inactivation, and down-regulation of the phosphate transport porin phoe.clinical isolates of klebsiella pneumoniae resistant to carbapenems and essentially all other antibiotics (multidrug resistant) are being isolated from some hospitals in new york city with increasing frequency. a highly related pair of k. pneumoniae strains isolated on the same day from one patient in a hospital in new york city were studied for antibiotic resistance. one (kp-2) was resistant to imipenem, meropenem, and sulopenem (mics of 16 to 32 microg/ml) while the other (kp-1) was susceptibl ...200617005822
antimicrobial susceptibility among pathogens collected from hospitalized patients in the united states and in vitro activity of tigecycline, a new glycylcycline antimicrobial.the activities of tigecycline and comparators against isolates collected from 76 u.s. centers between january 2004 and september 2005 were assessed. tigecycline mic(90)s were < or =2 microg/ml for klebsiella pneumoniae, klebsiella oxytoca, escherichia coli, enterobacter aerogenes, enterobacter cloacae, serratia marcescens, acinetobacter baumannii, staphylococcus aureus, enterococcus faecalis, enterococcus faecium, and streptococcus agalactiae.200617005838
mercuric reductase gene transfer from soil to rumen bacteria.conjugal transfer between soil bacterial population and microorganisms isolated from the rumen of herbivores from mercury-polluted area was investigated. the transfer of mera encoding mercury-resistance plasmids from soil bacteria enterobacter cloacae and enterococcus durans into two ruminal isolates citrobacter freundii and bacillus subtilis was observed. approximately the same frequency of mobilization in mating experiments was observed for both gram-negative (approximately 2.5 x 10(-8), trans ...200617007435
screening for anti-infective properties of several medicinal plants of the mauritians flora.several plants of the mauritian flora alleged to possess anti-infective properties were studied against different strains of pathogenic bacteria and fungi. the grounded dried plant materials were extracted with different extractants and screened for anti-microbial activity using the disk diffusion and the micro-dilution techniques. preliminary screening revealed that the methanol extracts were most active. salmonella enteritidis, enterobacter cloacae and bacillus subtilis were the three test org ...200717011733
aerobic growth of escherichia coli with 2,4,6-trinitrotoluene (tnt) as the sole nitrogen source and evidence of tnt denitration by whole cells and cell-free extracts.escherichia coli grew aerobically with 2,4,6-trinitrotoluene (tnt) as sole nitrogen source and caused tnt's partial denitration. this reaction was enhanced in nongrowing cell suspensions with 0.516 mol nitrite released per mol tnt. cell extracts denitrated tnt in the presence of nad(p)h. isomers of amino-dimethyl-tetranitrobiphenyl were detected and confirmed with u-15n-labeled tnt.200617012591
etiological agents of dairy cows' mastitis in western part of poland.the purpose of the study was to determine the etiological mastitis agents in cows from herds located in the western part of poland in years 2003-2005. bacteriological examinations of 18,713 samples taken from california mastitis test (cmt) positive quarters were performed according to standard methods. some gram-negative bacilli and gram-positive cocci were examined with the use of api tests (analytical profile index). it was found that 32.7% of samples that were cmt positive were culture negati ...200617020014
evaluation of the bd phoenix automated microbiology system for identification and antimicrobial susceptibility testing of enterobacteriaceae.we evaluated the accuracy of the bd phoenix system for the identification (id) and antimicrobial susceptibility testing (ast) of 251 isolates of the family enterobacteriaceae representing 31 species. organisms were inoculated onto the phoenix panel according to the manufacturer's instructions. the results from conventional biochemical tests were used for the reference method for id. agar dilution, performed according to the clsi guidelines, was the reference ast method. essential and categorical ...200617021074
mutation in cyaa in enterobacter cloacae decreases cucumber root colonization.strains of enterobacter cloacae show promise as biological control agents for pythium ultimum-induced damping-off on cucumber and other crops. enterobacter cloacae m59 is a mini-tn5 km transposon mutant of strain 501r3. populations of m59 were significantly lower on cucumber roots and decreased much more rapidly than those of strain 501r3 with increasing distance from the soil line. strain m59 was decreased or deficient in growth and chemotaxis on most individual compounds detected in cucumber r ...200717024489
development of a viologen-based microtiter plate assay for the analysis of oxyanion reductase activity: application to the membrane-bound selenate reductase from enterobacter cloacae sld1a-1.the membrane-bound selenate reductase of enterobacter cloacae sld1a-1 is purified in low yield and has relatively low activity in the pure form compared to that of other oxyanion reductases, such as the membrane-bound and periplasmic nitrate reductases. a microtiter plate assay based on the original quartz cuvette viologen assay of jones and garland (r.w. jones, p.b. garland, biochem. j 164 (1977) 199-211) was developed specifically for analysis of such low-abundant, labile oxyanion reductases. ...200617027906
high prevalence of escherichia coli belonging to the b2+d phylogenetic group in inflammatory bowel disease.it is not clear which species of bacteria may be involved in inflammatory bowel disease (ibd). one way of determining which bacteria might be likely candidates is to use culture-independent methods to identify microorganisms that are present in diseased tissues but not in controls.200717028128
high prevalence of escherichia coli belonging to the b2+d phylogenetic group in inflammatory bowel disease.it is not clear which species of bacteria may be involved in inflammatory bowel disease (ibd). one way of determining which bacteria might be likely candidates is to use culture-independent methods to identify microorganisms that are present in diseased tissues but not in controls.200717028128
assessment of analysis of urinary pneumococcal antigen by immunochromatography for etiologic diagnosis of community-acquired pneumonia in adults.the limitations of conventional microbiologic methods (cmm) for etiologic diagnosis of community pneumococcal pneumonia have made faster diagnostic techniques necessary. our aim was to evaluate the usefulness of the immunochromatography (ict) technique for detecting urinary streptococcus pneumoniae antigen in the etiologic diagnosis of community-acquired pneumonias (cap). this was a prospective study on in-patients with cap in a tertiary hospital conducted from october 2000 to march 2004. apart ...200617028212
carbon : nitrogen : phosphorus ratios influence biofilm formation by enterobacter cloacae and citrobacter freundii.to test the effects of c : n : p ratio modification of a well-known nutrient medium formulation, the endo formulation on biofilm formation by enterobacter cloacae ecl and citrobacter freundii cf1 in both single-species and binary species biofilms.200617040234
bactericidal activity of ertapenem against major intra-abdominal pathogens.treatment of intra-abdominal infections remains a challenge owing to their polymicrobial nature and associated mortality risk. treatment regimens must provide broad-spectrum coverage, including gram-positive and gram-negative aerobic and anaerobic bacteria of gastrointestinal origin. ertapenem is a long-acting 1-beta-methyl parenteral group 1 carbapenem antibiotic that has a broad antibacterial spectrum and once-daily dosing supported by clinical studies. it is active against gram-positive and g ...200617045463
[characteristics of war wound infection].war wounds are the most complex type of non-targeted injuries due to uncontrolled tissue damage of varied and multifold localizations, exposing sterile body areas to contamination with a huge amount of bacteria. wound contamination is caused by both the host microflora and exogenous agents from the environment (bullets, cloth fragments, dust, dirt, water) due to destruction of the host protective barriers. war wounds are the consequence of destructive effects of various types of projectiles, whi ...200617048790
[development of beta-lactamase resistance in enterobacteria].enterobacteria produce elementary chromosomal enzymes, beta-lactamases of class a: tem and shv (escherichia coli, klebsiella pneumoniae). these can give rise to plasmid-coded broad-spectrum beta-lactamases (esbl) discovered in 1980 (e. coli, k. pneumoniae, enterobacter cloacae). the first cefotaximase (ctx-m, men-1) was reported in europe in 1990. this enzyme is far more active against cefotaxime than against ceftazidime and aztreonam. chromosomal hyperpoduction of k1 beta-lactamase differs from ...200617051471
first report of plasmid-mediated resistance to quinolones and cefotaxime in an enterobacter cloacae strain isolated from an outpatient in brazil. 200717060528
patterns of antimicrobial resistance in a surgical intensive care unit of a university hospital in turkey.several studies have reported higher rates of antimicrobial resistance among isolates from intensive care units than among isolates from general patient-care areas. the aims of this study were to review the pathogens associated with nosocomial infections in a surgical intensive care unit of a university hospital in turkey and to summarize rates of antimicrobial resistance in the most common pathogens. the survey was conducted over a period of twelve months in a tertiary-care teaching hospital lo ...200617064415
high prevalence of ceftazidime-resistant klebsiella pneumoniae and increase of imipenem-resistant pseudomonas aeruginosa and acinetobacter spp. in korea: a konsar program in 2004.a nationwide antimicrobial resistance surveillance has been conducted since 1997 in korea. in this study, susceptibility test data generated in 2004 by konsar group hospitals were analyzed and compared to those at a commercial laboratory. in hospitals, the rank orders of organisms in 2004 were identical to those in 2003. the most prevalent species was staphylococcus aureus (20.2%) in hospitals, but escherichia coli (29.7%) in the commercial laboratory. the proportions of enterococcus faecium to ...200617066507
the biochemical differentiation of enterobacter sakazakii genotypes.enterobacter sakazakii is an emergent pathogen that has been associated with neonatal infections through contaminated powdered infant milk formula. the species was defined by farmer et al. (1980) who described 15 biogroups according to the biochemical characterization of 57 strains. this present study compares genotypes (dna cluster groups based on partial 16s rdna sequence analysis) with the biochemical traits for 189 e. sakazakii strains.200617067387
sensitive quantitative detection of commensal bacteria by rrna-targeted reverse transcription-pcr.a sensitive rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) method was developed for exact and sensitive enumeration of subdominant bacterial populations. using group- or species-specific primers for 16s or 23s rrna, analytical curves were constructed for escherichia coli, enterococcus faecalis, staphylococcus aureus, clostridium perfringens, and pseudomonas aeruginosa, and the threshold cycle value was found to be linear up to an rna amount of 10(-3) cell per rt-pcr. the number o ...200717071791
sensitive quantitative detection of commensal bacteria by rrna-targeted reverse transcription-pcr.a sensitive rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) method was developed for exact and sensitive enumeration of subdominant bacterial populations. using group- or species-specific primers for 16s or 23s rrna, analytical curves were constructed for escherichia coli, enterococcus faecalis, staphylococcus aureus, clostridium perfringens, and pseudomonas aeruginosa, and the threshold cycle value was found to be linear up to an rna amount of 10(-3) cell per rt-pcr. the number o ...200717071791
comparison of media for the isolation of enterobacter sakazakii.enterobacter sakazakii is associated with neonatal infections and is occasionally present at low levels (<1 cfu/g) in powdered infant formula milk (ifm). it has been previously reported that some e. sakazakii strains do not grow in standard media for enterobacteriaceae and coliform bacteria; therefore, a reliable method is needed for recovery of the organism. three e. sakazakii enrichment broths-enterobacteriaceae enrichment broth (ee), e. sakazakii selective broth (essb), and modified lauryl su ...200717071794
comparison of media for the isolation of enterobacter sakazakii.enterobacter sakazakii is associated with neonatal infections and is occasionally present at low levels (<1 cfu/g) in powdered infant formula milk (ifm). it has been previously reported that some e. sakazakii strains do not grow in standard media for enterobacteriaceae and coliform bacteria; therefore, a reliable method is needed for recovery of the organism. three e. sakazakii enrichment broths-enterobacteriaceae enrichment broth (ee), e. sakazakii selective broth (essb), and modified lauryl su ...200717071794
are laboratory-based antibiograms reliable to guide the selection of empirical antimicrobial treatment in patients with hospital-acquired infections?antibiograms are often taken into account to define a rational selection of an empirical antimicrobial therapy for treating patients with hospital-acquired infections. in this study, we performed a paired comparison between the antibiogram constructed with laboratory-based data and that formed with data subjected to prior clinical validation.200717079239
identification of ctx-m-type extended-spectrum-beta-lactamase genes using real-time pcr and pyrosequencing.ctx-m extended-spectrum beta-lactamases (esbls) are increasingly prevalent worldwide among escherichia coli bacteria, mostly in community-acquired urinary tract infections. finding a fast and reliable technique for identification of ctx-m enzymes is becoming a challenge for the microbiology laboratory. a fast real-time pcr amplification technique, using degenerated primers specific for all the bla(ctx-m) alleles, coupled to real-time pyrosequencing was developed. the five ctx-m groups were unamb ...200717088478
identification of ctx-m-type extended-spectrum-beta-lactamase genes using real-time pcr and pyrosequencing.ctx-m extended-spectrum beta-lactamases (esbls) are increasingly prevalent worldwide among escherichia coli bacteria, mostly in community-acquired urinary tract infections. finding a fast and reliable technique for identification of ctx-m enzymes is becoming a challenge for the microbiology laboratory. a fast real-time pcr amplification technique, using degenerated primers specific for all the bla(ctx-m) alleles, coupled to real-time pyrosequencing was developed. the five ctx-m groups were unamb ...200717088478
antibacterial activity of coffee extracts and selected coffee chemical compounds against enterobacteria.the in vitro antimicrobial activity of commercial coffee extracts and chemical compounds was investigated on nine strains of enterobacteria. the antimicrobial activity investigated by the disc diffusion method was observed in both the extracts and tested chemical compounds. even though ph, color, and the contents of trigonelline, caffeine, and chlorogenic acids differed significantly among the coffee extracts, no significant differences were observed in their antimicrobial activity. caffeic acid ...200617090115
rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time pcr assay and melt curve analysis.metallo-beta-lactamase enzymes (mbetal) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. the objective of this study was to develop a multiplex real-time pcr assay followed by a melt curve step for rapid detection and identification of genes encoding mbetal-type enzymes based on the amplicon melting peak. the reference sequences of all genes encoding imp and vim types, spm-1, gim-1, and sim-1 were downloaded from genbank, and primers were designed t ...200717093019
rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time pcr assay and melt curve analysis.metallo-beta-lactamase enzymes (mbetal) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. the objective of this study was to develop a multiplex real-time pcr assay followed by a melt curve step for rapid detection and identification of genes encoding mbetal-type enzymes based on the amplicon melting peak. the reference sequences of all genes encoding imp and vim types, spm-1, gim-1, and sim-1 were downloaded from genbank, and primers were designed t ...200717093019
combined use of pastorex staph-plus and either of two new chromogenic agars, mrsa id and chromagar mrsa, for detection of methicillin-resistant staphylococcus aureus.we describe the search toward a fast and reliable strategy to detect and confirm the presence of methicillin-resistant staphylococcus aureus (mrsa) in screening samples. first, we evaluated the sensitivities and specificities of oxacillin resistance screening agar (orsa) with enrichment (tryptic soy broth [tsb] and orsa [tsb-orsa]) and without enrichment (orsa), mrsa id (mrsa_id) plates, and chromagar mrsa (c_mrsa) plates, all of which were inoculated with equal volumes of a suspension made by e ...200717093032
combined use of pastorex staph-plus and either of two new chromogenic agars, mrsa id and chromagar mrsa, for detection of methicillin-resistant staphylococcus aureus.we describe the search toward a fast and reliable strategy to detect and confirm the presence of methicillin-resistant staphylococcus aureus (mrsa) in screening samples. first, we evaluated the sensitivities and specificities of oxacillin resistance screening agar (orsa) with enrichment (tryptic soy broth [tsb] and orsa [tsb-orsa]) and without enrichment (orsa), mrsa id (mrsa_id) plates, and chromagar mrsa (c_mrsa) plates, all of which were inoculated with equal volumes of a suspension made by e ...200717093032
novel ambler class a beta-lactamase lap-1 and its association with the plasmid-mediated quinolone resistance determinant qnrs1.the plasmid-mediated quinolone resistance determinant qnrs1 was identified in non-clonally related enterobacter cloacae isolates in association with a transferable narrow-spectrum beta-lactam resistance marker. cloning experiments allowed the identification of a novel ambler class a beta-lactamase, named lap-1. it shares 62 and 61% amino acid identity with the most closely related beta-lactamases, tem-1 and shv-1, respectively. it has a narrow-spectrum hydrolysis of beta-lactams and is strongly ...200717116662
bacteriological profile of neonatal septicaemia in a tertiary hospital in nigeria.neonatal septicaemia is an important cause of morbidity and mortality. knowledge of the bacteriological profile of the aetiologic agents is very important and helps to reduce the associated mortality in neonatal septicaemia.200617140336
a tlc bioautographic assay for the detection of nitrofurantoin resistance reversal compound.a simple tlc bioautographic method was developed for detection of antibiotic resistance reversal agents. in this study, the retention factor values of the components of some essential oils not previously shown to have any antibacterial activity were evaluated on nitrofurantoin supplemented agar media. the active component of artemisia annua, artemisia dracunculus and eucalyptus globulus essential oils was piperitone which increased the antibacterial activity of nitrofurantoin against enterobacte ...200717140862
plasmid-mediated kpc-2 in a klebsiella pneumoniae isolate from china.a carbapenem-resistant isolate of klebsiella pneumoniae producing class a carbapenemase kpc-2 was identified in zhejiang, china. the kpc-2 gene was located on an approximately 60-kb plasmid in a genetic environment partially different from that of blakpc-2 in the isolates from the united states and colombia.200717145797
plasmid-mediated kpc-2 in a klebsiella pneumoniae isolate from china.a carbapenem-resistant isolate of klebsiella pneumoniae producing class a carbapenemase kpc-2 was identified in zhejiang, china. the kpc-2 gene was located on an approximately 60-kb plasmid in a genetic environment partially different from that of blakpc-2 in the isolates from the united states and colombia.200717145797
detection of treponema pallidum subsp. pallidum from skin lesions, serum, and cerebrospinal fluid in an infant with congenital syphilis after clindamycin treatment of the mother during pregnancy.we report here a case of congenital syphilis in a newborn after clindamycin treatment in pregnancy. using pcr detection of tmpc (tp0319) and dna sequencing of the genes tp0136 and tp0548, dna sequences identical to treponema pallidum subsp. pallidum strain ss14 were detected in the infant's skin lesions, serum, and cerebrospinal fluid.200717151205
detection of treponema pallidum subsp. pallidum from skin lesions, serum, and cerebrospinal fluid in an infant with congenital syphilis after clindamycin treatment of the mother during pregnancy.we report here a case of congenital syphilis in a newborn after clindamycin treatment in pregnancy. using pcr detection of tmpc (tp0319) and dna sequencing of the genes tp0136 and tp0548, dna sequences identical to treponema pallidum subsp. pallidum strain ss14 were detected in the infant's skin lesions, serum, and cerebrospinal fluid.200717151205
a robotic dna purification protocol and real-time pcr for the detection of enterobacter sakazakii in powdered infant formulae.enterobacter sakazakii is the causative agent of rare but severe food-borne infections associated with meningitis, necrotizing enterocolitis and sepsis in infants. rehydrated powdered infant formulae have been implicated as the source of infection in several outbreaks and sporadic cases. in this work, a real time fluorescence resonance energy transfer pcr assay incorporating an internal amplification control (iac) was developed for the specific detection of e. sakazakii in foods. performance of ...200617166252
[antimicrobial resistance in gram negative bacteria isolated from intensive care units of colombian hospitals, whonet 2003, 2004 and 2005].surveillance systems play a key role in the detection and control of bacterial resistance. it is necessary to constantly collect information from all institutions because the mechanisms of bacterial resistance can operate in different ways between countries, cities and even in hospitals in the same area. therefore local information is important in order to learn about bacterial behaviour and design appropriate interventions for each institution. between january 2003 and december 2004, the centro ...200617176006
[fever, dyspnea and bacteriemia due to klebsiella oxytoca and enterobacter cloacae]. 200617178084
surveillance of antimicrobial use and antimicrobial resistance in german intensive care units (sari): a summary of the data from 2001 through 2004.to report the experience gained over 4 years in working with the german sari project (surveillance of antimicrobial use and antimicrobial resistance in intensive care units), and to compare sari with data from the swedish strama and the us aur surveillance system.200617180583
evaluation of a new selective chromogenic agar medium for detection of extended-spectrum beta-lactamase-producing enterobacteriaceae.a novel chromogenic agar medium (esbl-bx; biomérieux, marcy l'etoile, france) was compared to macconkey agar supplemented with 2 mg ceftazidime/liter (mckc) for the selective isolation and presumptive identification of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae directly from clinical samples. of a total of 644 clinical specimens (including 551 fecal samples), 496 yielded no growth and 148 yielded growth on one or both media. overall, 44 esbl-producing enterobacteriaceae ...200717182756
evaluation of a new selective chromogenic agar medium for detection of extended-spectrum beta-lactamase-producing enterobacteriaceae.a novel chromogenic agar medium (esbl-bx; biomérieux, marcy l'etoile, france) was compared to macconkey agar supplemented with 2 mg ceftazidime/liter (mckc) for the selective isolation and presumptive identification of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae directly from clinical samples. of a total of 644 clinical specimens (including 551 fecal samples), 496 yielded no growth and 148 yielded growth on one or both media. overall, 44 esbl-producing enterobacteriaceae ...200717182756
enterobacteriaceae in dehydrated powdered infant formula manufactured in indonesia and malaysia.to determine the occurrence of salmonella and shigella in infant formula from southeast asia, 74 packages of dehydrated powdered infant follow-on formula (recommended age, > 4 months) from five different manufacturers, four from indonesia and one from malaysia, were analyzed. none of the 25-g test portions yielded salmonella or shigella. however, further identification of colonies growing on selective media used for salmonella and shigella detection revealed the frequent occurrence of several ot ...200617186672
biodiversity of bacterial ecosystems in traditional egyptian domiati cheese.bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ...200717189434
biodiversity of bacterial ecosystems in traditional egyptian domiati cheese.bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ...200717189434
dual detection of fungal infections in drosophila via recognition of glucans and sensing of virulence factors.the drosophila immune system discriminates between various types of infections and activates appropriate signal transduction pathways to combat the invading microorganisms. the toll pathway is required for the host response against fungal and most gram-positive bacterial infections. the sensing of gram-positive bacteria is mediated by the pattern recognition receptors pgrp-sa and gnbp1 that cooperate to detect the presence of infections in the host. here, we report that gnbp3 is a pattern recogn ...200617190605
[resistance to cefepime in enterobacter cloacae isolates from hospitals in bogotá, colombia].the aim of this research was to detect the presence of genes encoding beta-lactamases which can confer cefepime resistance on enterobacter cloacae isolates, meaning that this antibiotic may be considered an important therapeutic alternative.200617191603
molecular modeling of henry-michaelis and acyl-enzyme complexes between imipenem and enterobacter cloacae p99 beta-lactamase.we report a molecular-mechanics (amber*) study on the henry-michaelis complex and the corresponding acyl-enzyme adduct formed between imipenem (1), a transient inhibitor of beta-lactamases, and enterobacter cloacae p99, a class c-beta-lactamase. we have examined the influence of the structural configuration of the functional groups in the substrate on their three-dimensional (3d) arrangement at the active site, which was compared with those adopted by typical penicillins and cephalosporins. our ...200517192008
comparison of essential oils from three plants for enhancement of antimicrobial activity of nitrofurantoin against enterobacteria.piperitone from plant essential oils enhancesbactericidal activities of nitrofurantoin and furazolidone against bacteria from the family enterobacteriaceae. in this study, the essential oils of spearmint (mentha spicata l.), dill (anethum graveolens l.) and peppermint (mentha piperita l.)were screened for augmentation of nitrofurantoin activity and the most active components were determined.200717192709
transposon tn7 is widespread in diverse bacteria and forms genomic islands.we find that relatives of the bacterial transposon tn7 are widespread in disparate environments and phylogenetically diverse species. these elements form functionally diverse genomic islands at the specific site of tn7 insertion adjacent to glms. this work presents the first example of genomic island formation by a dde type transposon.200717194796
transposon tn7 is widespread in diverse bacteria and forms genomic islands.we find that relatives of the bacterial transposon tn7 are widespread in disparate environments and phylogenetically diverse species. these elements form functionally diverse genomic islands at the specific site of tn7 insertion adjacent to glms. this work presents the first example of genomic island formation by a dde type transposon.200717194796
accelerated detection of extended-spectrum beta-lactamases in clinical isolates of enterobacteriaceae.a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum beta-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esbl-producers by the standard clinical laboratory stand ...200617201201
rapid genotyping of ctx-m extended-spectrum beta-lactamases by denaturing high-performance liquid chromatography.denaturing high-performance liquid chromatography (dhplc) is a powerful technique which has been used extensively to detect genetic variation. this is the first report of the application of dhplc for rapid genotyping of bacterial beta-lactamase genes. the technique was specifically developed to genotype members of all blactx-m dna homology groups. thirteen well-defined blactx-m extended-spectrum beta-lactamase (esbl)-producing strains were used to develop and optimize the dhplc genotyping assay. ...200717210774
native microbial colonization of drosophila melanogaster and its use as a model of enterococcus faecalis pathogenesis.enterococci are commensal organisms of the gastrointestinal (gi) tracts of a broad range of mammalian and insect hosts, but they are also leading causes of nosocomial infection. little is known about the ecological role of enterococci in the gi tract consortia. to develop a tractable model for studying the roles of these organisms as commensals and pathogens, we characterized the drosophila melanogaster microflora and examined the occurrence of enterococci in the gastrointestinal consortium of d ...200717220307
mosaic structure of p1658/97, a 125-kilobase plasmid harboring an active amplicon with the extended-spectrum beta-lactamase gene blashv-5.escherichia coli isolates recovered from patients during a clonal outbreak in a warsaw, poland, hospital in 1997 produced different levels of an extended-spectrum beta-lactamase (esbl) of the shv type. the beta-lactamase hyperproduction correlated with the multiplication of esbl gene copies within a plasmid. here, we present the complete nucleotide sequence of plasmid p1658/97 carried by the isolates recovered during the outbreak. the plasmid is 125,491 bp and shows a mosaic structure in which a ...200717220406
emergence of arma and rmtb aminoglycoside resistance 16s rrna methylases in belgium.16s rrna methylase-mediated high-level resistance to aminoglycosides has been reported recently in clinical isolates of gram-negative bacilli only from a limited number of countries. this study was conducted to investigate the occurrence of this type of resistance in clinical isolates of enterobacteriaceae from two belgian hospitals and the characteristics of the strains.200717224412
effect of a chronic nail-biting habit on the oral carriage of enterobacteriaceae.nail biting is a common oral habit in children and young adults. however, its effect on the oral carriage of enterobacteriaceae is unclear. the purpose of the study was to evaluate the differences in prevalence of enterobacteriaceae in saliva samples from subjects with and without a nail-biting habit.200717241163
prevalence of plasmid-mediated quinolone resistance determinants qnra, qnrb, and qnrs among clinical isolates of enterobacter cloacae in a taiwanese hospital.the prevalence of three plasmid-mediated quinolone resistance determinants, qnra, qnrb, and qnrs, among 526 nonreplicate clinical isolates of enterobacter cloacae collected at a taiwanese university hospital in 2004 was determined by pcr and colony hybridization, and the association of qnr with the imp-8 metallo-beta-lactamase was investigated. eighty-six (16.3%) of all isolates were qnr positive, and the qnra1-like, qnrb2-like, and qnrs1-like genes were detected alone or in combination in 3 (0. ...200717242140
predictors of in-hospital mortality for bloodstream infections caused by enterobacter species or citrobacter freundii.to identify predictors of in-hospital mortality among patients with bacteremia caused by enterobacter cloacae, enterobacter aerogenes, or citrobacter freundii.200717253909
type ii topoisomerase mutations in clinical isolates of enterobacter cloacae and other enterobacterial species harbouring the qnra gene.the qnr genes are transferable genes that confer low-level quinolone resistance by protection of topoisomerase. the occurrence of mutations in dna gyrase (gyra, gyrb) and topoisomerase iv (parc, pare) genes in strains harbouring qnr was investigated in 28 qnra-positive clinical isolates, among which 7 strains also harboured qnrs. topoisomerase mutations were found in 25 (89%) of the 28 strains, with at least two mutations (gyra and parc) in 13 strains and one mutation in 12 strains. isolates of ...200717254753
bioassay for determination of fosmidomycin in plasma and urine: application for pharmacokinetic dose optimisation.a simple, sensitive, selective and reproducible method based on agar diffusion disk assay was developed for the determination of fosmidomycin and clindamycin in human plasma and urine. a disk diffusion technique was used, essentially as previously described but utilising the assay organism enterobacter cloacae atcc 23355 strain to seed the agar assay plates. calibration curves were prepared from concentration response curves in plasma (0, 1, 2.5, 5, 7.5, 10, 25, 50 ng/microl) and urine (0, 10, 2 ...200717258829
monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16s rrna gene-based analyses.the diversity and dynamics of bacterial populations in saint-nectaire, a raw-milk, semihard cheese, were investigated using a dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (sscp) fingerprinting and sequencing of 16s rrna genes. the dominant clones, among 125 16s rrna genes isolated from milk, belonged to members of the firmicutes (58% of the total clones) affiliated mainly with the orders clostridiales and the lactobacillales, followed by ...200717259356
se(vi) reduction and the precipitation of se(0) by the facultative bacterium enterobacter cloacae sld1a-1 are regulated by fnr.the fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and se(0) precipitation. in this study, we identified a genetic regulator that controls selenate reductase activity in the se-reducing bacterium enterobacter cloacae sld1a-1. heterologous expression of the global anaerobic regulatory gene fnr (fumarate nitrate reduction regulator) from e. cloacae in the non-se-reducing strain escherichia coli s17-1 activated the ability to reduce se(vi) and p ...200717261520
in vitro and in vivo activities of pd 0305970 and pd 0326448, new bacterial gyrase/topoisomerase inhibitors with potent antibacterial activities versus multidrug-resistant gram-positive and fastidious organism groups.pd 0305970 and pd 0326448 are new bacterial gyrase and topoisomerase inhibitors (quinazoline-2,4-diones) that possess outstanding in vitro and in vivo activities against a wide spectrum of bacterial species including quinolone- and multidrug-resistant gram-positive and fastidious organism groups. the respective mics (microg/ml) for pd 0305970 capable of inhibiting>or=90% of bacterial strains tested ranged from 0.125 to 0.5 versus staphylococci, 0.03 to 0.06 versus streptococci, 0.25 to 2 versus ...200717261623
bacterial isolates from severe infections and their antibiotic susceptibility patterns in italy: a nationwide study in the hospital setting.the most frequent agents of severe bacterial infections and their antibiotic susceptibility patterns were determined in patients admitted to 45 italian hospitals over the years 2002-2003. the most common diagnoses were: sepsis (33.8%), pneumonia (9.4%), intravascular catheter-associated infections (9.3%) and ventilator-associated pneumonia (8.1%). overall, 5115 bacterial isolates were identified from 4228 patients. three bacterial species, staphylococcus aureus, pseudomonas aeruginosa and escher ...200617267336
septic shock after intracervical laminaria insertion.laminaria placement is seldom thought to be associated with postabortal sepsis.200617272216
frequency of detection of periodontopathic and superinfecting bacteria in hiv-positive patients with periodontitis.the aim of this investigation was to establish the frequency of detection of periodontopathic bacteria and gram-negative enteric rods in hiv-positive (hiv+) patients with periodontitis.200717274235
the endemic situation of enterobacter aerogenes and enterobacter cloacae: you will only discover what you are looking for. 200717275960
surveillance of bacteriological quality of natural water resources in rural areas around kasauli town, distt. solan, himachal pradesh.a study was conducted to find out the bacteriological quality of water during the monsoon season in local areas around kasauli town, and to evaluate the management of water resources in rural areas. the water samples were collected from bowries and hand pumps. as there is no municipal water supply in majority of these areas, the local residents depends mainly on these natural resources without any treatment of water. all the 14 surface water samples collected from bowries were found bacteriologi ...200517278659
towards a ligand targeted enzyme prodrug therapy: single round panning of a beta-lactamase scaffold library on human cancer cells.a novel beta-lactamase scaffold library in which the target-binding moiety is built into the enzyme was generated using phage display technology. the binding element is composed of a fully randomized 8 amino acid loop inserted at position between y34 and k37 on the outer surface of enterobacter cloacae p99 cephalosporinase (beta-lactamase, e.c. 3.5.2.6) with all library members retaining catalytic activity. the frequency and diversity of amino acids distributions in peptide inserts from library ...200717285581
detection of extended-spectrum beta-lactamases among enterobacteriaceae by use of semiautomated microbiology systems and manual detection procedures.three commercially available microbiology identification and susceptibility testing systems were compared with regard to their ability to detect extended-spectrum beta-lactamase (esbl) production in enterobacteriaceae, i.e., the phoenix automated microbiology system (bd diagnostic systems, sparks, md), the vitek 2 system (biomérieux, marcy l'etoile, france), and the microscan walkaway-96 system (dade behring, inc., west sacramento, ca), using routine testing panels. one hundred fifty putative es ...200717287329
role of ceftazidime dose regimen on the selection of resistant enterobacter cloacae in the intestinal flora of rats treated for an experimental pulmonary infection.the effect of ceftazidime dosing increments and frequency of dosing on the selection of ceftazidime-resistant enterobacter cloacae in the intestine was studied in rats, during treatment of a pulmonary infection caused by klebsiella pneumoniae.200717289765
evaluating antibiotics for use in medicine using a poloxamer biofilm model.wound infections, due to biofilms, are a constant problem because of their recalcitrant nature towards antibiotics. appropriate antibiotic selection for the treatment of these biofilm infections is important. the traditional in vitro disc diffusion method for antibiotic selection uses bacterial cultures grown on agar plates. however, the form of bacterial growth on agar is not representative of how bacteria grow in wounds and other tissue sites as here bacteria grow naturally in a biofilm. the a ...200717302978
evaluation of phoenix automated microbiology system for detecting extended-spectrum beta-lactamases among chromosomal ampc-producing enterobacter cloacae, enterobacter aerogenes, citrobacter freundii, and serratia marcescens.we evaluated the bd phoenix extended-spectrum beta-lactamase (esbl) detection test among chromosomal ampc-producing enterobacter cloacae, enterobacter aerogenes, citrobacter freundii, and serratia marcescens. the study was conducted on 72 non-repetitive esbl producers (33 e. cloacae, 13 e. aerogenes, 14 c. freundii, and 12 s. marcescens) and 77 esbl non-producers (33 e. cloacae, 9 e. aerogenes, 6 c. freundii, and 29 s. marcescens). the organisms were selected as suspected esbl-producers based on ...200717311873
failure to control an outbreak of qnra1-positive multidrug-resistant enterobacter cloacae infection despite adequate implementation of recommended infection control measures.a large outbreak with an aminoglycoside-resistant enterobacter cloacae (arec) clone occurred at the university medical center utrecht beginning in 2001 and continued up through the time that this study was completed. this clone (genotype i) contains a conjugative r plasmid carrying the qnra1, bla(ctx-m-9), and aadb genes, encoding resistance to quinolones, extended-spectrum beta-lactamases, and aminoglycosides, respectively. the aim of this study was to determine whether this clone was more tran ...200717314218
identification of plasmid-mediated extended-spectrum and ampc beta-lactamases in enterobacter spp. isolated from dogs.the genetic determinants involved in reduced susceptibility to third-generation cephalosporins and aztreonam were identified in ten canine enterobacter isolates associated with opportunistic infections in three veterinary hospitals in brisbane, australia. all isolates were evaluated by a combination of phenotypic (broth microdilution and disc susceptibility, modified disc diffusion and ief) and genotypic (pfge, plasmid analysis, southern blot hybridization, bacterial conjugation, pcr and sequenc ...200717314376
role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils.heavy metal pollution of soil is a significant environmental problem and has its negative impact on human health and agriculture. rhizosphere, as an important interface of soil and plant, plays a significant role in phytoremediation of contaminated soil by heavy metals, in which, microbial populations are known to affect heavy metal mobility and availability to the plant through release of chelating agents, acidification, phosphate solubilization and redox changes, and therefore, have potential ...200717323432
ethnoveterinary medicines used for ruminants in british columbia, canada.the use of medicinal plants is an option for livestock farmers who are not allowed to use allopathic drugs under certified organic programs or cannot afford to use allopathic drugs for minor health problems of livestock.200717324258
crystal structure and functional characterization of a d-stereospecific amino acid amidase from ochrobactrum anthropi sv3, a new member of the penicillin-recognizing proteins.d-amino acid amidase (daa) from ochrobactrum anthropi sv3, which catalyzes the stereospecific hydrolysis of d-amino acid amides to yield the d-amino acid and ammonia, has attracted increasing attention as a catalyst for the stereospecific production of d-amino acids. in order to clarify the structure-function relationships of daa, the crystal structures of native daa, and of the d-phenylalanine/daa complex, were determined at 2.1 and at 2.4 a resolution, respectively. both crystals contain six s ...200717331533
bacterial resistance to silver in wound care and medical devices.this review discusses the molecular and genetic evidence for silver resistance in bacteria isolated from skin wounds and medical devices with reference to a case study of resistant enterobacter cloacae from the leg ulcers of an elderly woman.200717334141
characterization of extended-host-range pseudo-t-even bacteriophage kpp95 isolated on klebsiella pneumoniae.kpp95, isolated on klebsiella pneumoniae, is a bacteriophage with the morphology of t4-type phages and is capable of rapid lysis of host cells. its double-stranded genomic dna (ca. 175 kb, estimated by pulsed-field gel electrophoresis) can be cut only by restriction endonucleases with a cleavage site flanked either by a and t or by t, as tested, suggesting that it contains the modified derivative(s) of g and/or c. over 26 protein bands were visualized upon sodium dodecyl sulfate-polyacrylamide g ...200717337566
regulation of arsenate resistance in desulfovibrio desulfuricans g20 by an arsrbcc operon and an arsc gene.desulfovibrio desulfuricans g20 grows and reduces 20 mm arsenate to arsenite in lactate-sulfate media. sequence analysis and experimental data show that d. desulfuricans g20 has one copy of arsc and a complete arsrbcc operon in different locations within the genome. two mutants of strain g20 with defects in arsenate resistance were generated by nitrosoguanidine mutagenesis. the arsrbcc operons were intact in both mutant strains, but each mutant had one point mutation in the single arsc gene. mut ...200717337573
colicin biology.colicins are proteins produced by and toxic for some strains of escherichia coli. they are produced by strains of e. coli carrying a colicinogenic plasmid that bears the genetic determinants for colicin synthesis, immunity, and release. insights gained into each fundamental aspect of their biology are presented: their synthesis, which is under sos regulation; their release into the extracellular medium, which involves the colicin lysis protein; and their uptake mechanisms and modes of action. co ...200717347522
entomopathogenicity of native bacteria from anastrepha fraterculus and ceratitis capitata against the pest phyllocnistis citrella.in piura (peru), the pest phyllocnistis citrella stainton damages the photosynthetic rate and new bud production of citrus aurantiifolia swingle (sweet lemon), decreasing the yield, productivity and commercial price of its fruit. biological control was evaluated through the crossed effect of bacteria obtained from pests (anastrepha fraterculus wied., ceratitis capitata wied. and rhynchophorus palmarum l.) that are pathogenic against their original host species. enterobacter cloacae (jordan) horm ...200717348070
emergence of rmtb methylase-producing escherichia coli and enterobacter cloacae isolates from pigs in china.to investigate the occurrence of 16s rrna methylases conferring high-level resistance to aminoglycosides in enterobacteriaceae isolated from two pig farms in china.200717353219
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