Publications

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partial hydrolysis of dieldrin by aerobacter aerogenes. 19685647526
ionic relations as a function of metabolism in aerobacter aerogenes. 19685680993
mutants of aerobacter aerogenes capable of utilizing xylitol as a novel carbon.wild-type aerobacter aerogenes 1033 is unable to utilize xylitol. a succession of mutants was isolated capable of growth on this compound (0.2%) at progressively faster rates. whereas the ability to utilize xylitol was achieved in the first-stage mutant (x1) by constitutive production of ribitol dehydrogenase (for which xylitol is a substrate but not an inducer), the basis for enhanced utilization of xylitol in the second-stage mutant (x2) was an alteration of ribitol dehydrogenase. this enzyme ...19685674056
experimental mastitis in leukopenic cows: immunologically induced neutropenia and response to intramammary inoculation of aerobacter aerogenes. 19685685004
growth and bacteriolytic activity of a soil amoeba, hartmannella glebae.a soil amoeba, hartmannella glebae, could grow on a variety of gram-positive and gram-negative bacteria, although the rate of growth was faster in the presence of gram-negative bacteria. the amoeba, however, could not use yeasts, molds, or a green alga as a nutritional source. the extract prepared from amoebae grown in the presence of aerobacter aerogenes and alcaligenes faecalis could lyse intact cells and cell walls of many gram-positive bacteria at different rates. the spectrum of lytic activ ...19685651324
the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. ii. evidence that it is not a flavoprotein. 19685658549
the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. i. kinetic studies. 19685661161
the lipopolysaccharides of aerobacter aerogenes strains a3(s1) and nctc 243. 19685666760
the exopolysaccharide of klebsiella aerogens a3 (s1) (type 54). the isolation of o-acetylated octasaccharide, tetrasaccharide and trisaccharide.the exopolysaccharide slime produced by klebsiella aerogenes a 3 (sl) (type 54) is an o-acetylated polysaccharide, the components of which are glucose, glucuronic acid, fucose and acetyl in the molar proportions 4:2:2:1. a phage-induced fucosidase was obtained that hydrolyses the polysaccharide to give an octasaccharide having the same constituents in the same molar proportions. this octasaccharide (o3) is considered to be the repeating unit of the polysaccharide. it is hydrolysed by other phage ...19685704823
the influence of nacl concentration of the medium on the potassium content of aerobacter aerogenes and on the inter-relationships between potassium, magnesium and ribonucleic acid in the growing bacteria. 19685709275
differentiation of aerobacter-klebsiella isolated from sugarcane.three hundred and eighty-four isolates were obtained in the completed test portion of the most probable number determinations of coliforms in sugarcane sources. of these isolates, 88% were of the (- - + +) indole, methyl red, voges-proskauer, citrate (imvic) type and were identified as aerobacter aerogenes according to the protocol of the american public health association (1). employing 359 of these cultures, a comparative biochemical, serological, and pathogenicity study was carried out with k ...19685726159
regulation of nitrate assimilation and nitrate respiration in aerobacter aerogenes.the influence of growth conditions on assimilatory and respiratory nitrate reduction in aerobacter aerogenes was studied. the level of nitrate reductase activity in cells, growing in minimal medium with nitrate as the sole nitrogen source, was much lower under aerobic than anaerobic conditions. further, the enzyme of the aerobic cultures was very sensitive to sonic disintegration, as distinct from the enzyme of anaerobic cultures. when a culture of a. aerogenes was shifted from anaerobic growth ...19685726295
pathway of l-mannose degradation in aerobacter aerogenes. 19685726889
studies on the ribosomes and ribonucleic acids of aerobacter aerogenes gron at different rates in carbon-limited continuous culture. 19685727125
studies on the ribosomes and ribonulceic acids of aerobacter aerogenes grown at different rates in magnesium-limited continuous culture. 19685727126
lipoidal components of bacterial lipopolysaccharides: nature and distribution of fatty acids in aerobacter aerogenes.the fatty acid distribution of aerobacter aerogenes was studied by comparing the fatty acid composition of the lipoidal component of the endotoxin (lipid a) with the fatty acids of the readily extractable native lipids and total cellular fatty acids. the results for total cellular fatty acids and readily extractable native lipids were generally similar, but both quantitative and qualitative differences exist. in addition, profound differences between these two fractions and lipid a were observed ...19685732501
multiple liver abscesses caused by klebsiella aerogenes. 19685748362
[aerobacter aerogenes as the cause of lethal sepsis in a small baby]. 19685675545
incidence of intramammary infections during lactation in dairy cattle repeatedly exposed to streptococcus agalactiae and aerobacter aerogenes. 19685690683
[aerobacter aerogenes peritonitis in infancy and childhood]. 19685700570
inhibition of glycerol dehydrogenase from aerobacter aerogenes by dihydroxyacetone, high ionic strength, and monovalent cations. 19685657454
the biosynthesis of tyrosine in aerobacter aerogenes. evidence for a suunit structure of the protein converting chorismate into 4-hydroxyphenylpyruvate. 19685658122
an enzyme system for de novo biosynthesis of glycogen in aerobacter aerogenes. 19685749173
action of urea on the activity of dehydrogenases and alpha glucosidase in aerobacter aerogenes grown in continous culture. 19695765516
peptide hydrolase activity of klebsiella aerogenes. 19695767044
bactericidal activity of normal milk, mastitic milk, and colostrum against aerobacter aerogenes. 19695815918
the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. determination and function of thiol groups. 19695823100
the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. molecular properties. 19695823101
effect of growth rate on histidine catabolism and histidase synthesis in aerobacter aerogenes.a study was made of how the catabolism of a carbon and energy source is affected by the biosynthetic demands of growing bacterial cells. cultures of aerobacter aerogenes in l-histidine medium were grown in a chemostat at rates determined by the supply of either sulfate or a required amino acid, l-arginine. it was discovered that the rate at which these cells grow under a biosynthetic restriction determines both the rate and the pattern of histidine degradation. (i) histidine catabolism is partia ...19695781570
regulation of gratuitous beta-galactosidase synthesis in aerobacter aerogenes during an adaptive process. 19695783886
theory of oscillations of respiration rate in continuous culture of klebsiella aerogenes. 19695783912
requirement for sodium in the anaerobic growth of aerobacter aerogenes on citrate.anaerobic growth of aerobacter aerogenes on citrate as a carbon source required the presence of na(+). the growth rate increased with increasing na(+) concentration and was optimal at 0.10 m na(+). the requirement was specific for na(+), which could not be replaced by k(+), nh(4) (+), li(+), rb(+), or cs(+). k(+) was required for growth in the presence of na(+), the optimal k(+) concentration being 0.15 mm. enzyme profiles were determined on cells grown in three different media: (i) intermediate ...19695784198
role of sodium in determining alternate pathways of aerobic citrate catabolism in aerobacter aerogenes.in contrast to the absolute na(+) requirement for anaerobic growth of aerobacter aerogenes on citrate as sole carbon source, aerobic growth of this microorganism did not require the presence of na(+). however, na(+) (optimal concentration, 10 mm) did increase the maximal amount of aerobic growth by 60%, even though it did not change the rate of growth. this increase in growth was specifically affected by na(+), which could not be replaced by k(+), nh(4) (+), li(+), rb(+), or cs(+). enzyme profil ...19695808070
induction and properties of the citrate transport system in aerobacter aerogenes.the mediated transport of citrate in aerobacter aerogenes was studied. according to data obtained by examining the distribution of radioactive citrate at room temperature and at 0 c, a carrier system appears to be located on the membrane. the carrier system is inducible and very specific, not acting on the related compounds isocitrate and cis-aconitate. induction required synthesis of both ribonucleic acid and protein as determined by starving auxotrophic mutants and by using specific inhibitors ...19695784212
control of synthesis of malate dehydrogenase in aerobacter aerogenes.an inverse linear relationship was observed between the levels of l-malate dehydrogenase (mdh) and growth rates of aerobacter aerogenes when grown under aerobic and anaerobic conditions on various substrates which served as the sole carbon and energy sources. deviations from this linearity were found. mdh levels of cells grown aerobically on oxalacetate, l-malate, d-mannose and d-galactose, and of cells grown anaerobically on l-malate and d-mannose were higher than those expected according to th ...19695354938
basis for the mutational acquisition of the ability of aerobacter aerogenes to grow on l-mannose.growth of aerobacter aerogenes prl-r3 on the unnatural hexose l-mannose as a sole carbon source is dependent upon the selection of a mutant. growth of the mutant on l-mannose did not require the synthesis of novel enzymes for the degradation of l-mannose, since enzymes of the l-rhamnose degradative pathway could serve this function. however, unlike most other apparent gain mutations that have been described, the mutant was not constitutive for the degradative enzymes; isomerase, kinase, and aldo ...19695354955
the non-oxidative decarboxylation of p-hydroxybenzoic acid, gentisic acid, protocatechuic acid and gallic acid by klebsiella aerogenes (aerobacter aerogenes). 19695309907
the formation of phenol in the degradation of p-hydroxybenzoic acid by klebsiella aerogenes (aerobacter aerogenes). 19695305796
the influence of growth conditions on the composition of some cell wall components of aerobacter aerogenes. 19695361170
n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis and lethality in aerobacter (klebsiella) aerogenes. 19695367412
organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.ultracentrifugation in sucrose density gradients was employed to estimate the molecular weights and to determine possible physical aggregation of the five enzymes catalyzing steps two to six in the prechorismic acid portion of the polyaromatic synthetic pathway in six species of bacteria: escherichia coli, salmonella typhimurium, aerobacter aerogenes, bacillus subtilis, pseudomonas aeruginosa, and streptomyces coelicolor. the five enzymes were not aggregated in extracts of any of the species exa ...19694979440
enzymatic analysis of the requirement for sodium in aerobic growth of salmonella typhimurium on citrate.na(+) was required for the aerobic growth of salmonella typhimurium on citrate, but not on l-malate, glucose, or glycerol. the maximal growth rate and the maximal total growth occurred with 6 to 7 mm na(+). na(+) could not be replaced by k(+), nh(4) (+), li(+), rb(+), or cs(+). sonically treated extracts of citrate-grown cells contained the enzymes of the citrate fermentation pathway (citritase and oxalacetate decarboxylase) and all of the enzymes of the citric acid cycle. thus, two separate rou ...19694980062
capacity of aspartic acid to increase the bacterial count on suspensions of escherichia coli after freezing.the addition of 2% trypticase to a minimal salts-glucose plating medium increased the bacterial count of frozen and thawed suspensions of escherichia coli 451b cells, even when precautions were taken to remove toxic trace elements from the plating diluent. hydrolysis of the trypticase with hcl or h(2)so(4) reduced its count-increasing activity. treatment of the h(2)so(4) hydrolysate with a cation-exchange resin greatly improved its capacity to replace trypticase. addition of a mixture of amino a ...19694977483
inhibitory effects of ethidium bromide on the growth kinetics of klebsiella aerogenes, adaptation to ethidium and cross-resistance to proflavine. 19694917184
immunochemical and enzymatic comparisons of the tryptophan synthase alpha subunits from five species of enterobacteriaceae.the reactive surface structures of alpha subunits of tryptophan synthase from escherichia coli, shigella dysenteriae, salmonella typhimurium, aerobacter aerogenes, and serratia marcescens were compared by measuring (i) their reactivities in micro-complement-fixation assays with antibodies directed specifically to e. coli wild-type alpha subunit, (ii) their reactivities in enzyme neutralization assays with the same antibodies, and (iii) their binding affinities for tryptophan synthase beta(2) sub ...19694887511
comparison in normal and leukopenic cows of experimental mastitis due to aerobacter aerogenes or escherichia coli endotoxin. 19694888853
oxidation d-malic and beta-alkylmalic acids wild-type and mutant strains of salmonella typhimurium and by aerobacter aerogenes.a mutant strain of salmonella typhimurium (sl 1634 dml-51) capable of growth on d-malate as sole carbon source was shown to produce d-malic enzyme. this enzyme was absent in the parent wild-type strain which was unable to grow on d-malate. growth of the mutant on d-malate also resulted in a greatly increased level of beta-isopropylmalic enzyme compared with its level in the wild-type strain grown on citrate or l-malate. the d-malic and beta-isopropylmalic enzymes, both of which catalyze a nicoti ...19694889267
polynucleotide sequence relationships among members of enterobacteriaceae.polynucleotide relationships were examined among many representatives of the enterobacteriaceae by means of agar, membrane filter, and hydroxyapatite procedures. the amount of deoxyribonucleic acid (dna) that reassociated was dependent, especially in interspecific reactions, on the annealing temperature. in only three cases: escherichia coli-shigella flexneri, salmonella typhimurium-s. typhi, and proteus mirabilis-p. vulgaris, was relative interspecific duplex formation 80% or higher. in most ca ...19694891264
taxonomic investigations on expressed and cryptic phospho-beta-glucosidases in enterobacteriaceae.in the enterobacteriaceae, beta-glucosides are catabolized by a complex system formed of three permeases, with partly overlapping substrate specificities, and two hydrolytic enzymes, phospho-beta-glucosidase a and b, which hydrolyze only phosphorylated beta-glucosides. some enterobacteriaceae such as klebsiella-aerobacter (enterobacter) possess the complete system; others possess only parts of it or may have a cryptic phospho-beta-glucosidase activity without permease activity. a screening test ...19694897109
structural analysis of colanic acid from escherichia coli by using methylation and base-catalysed fragmentation. comparison with polysaccharides from other bacterial sources.essentially the same methanolysis products were obtained after methylation of the slime and capsular polysaccharides from escherichia coli k12 (s53 and s53c sub-strains) and the slime polysaccharides from e. coli k12 (s61), aerobacter cloacae n.c.t.c. 5290 and salmonella typhimurium sl1543. these were the methyl glycosides of 2-o-methyl-l-fucose, 2,3-di-o-methyl-l-fucose, 2,3-di-o-methyl-d-glucuronic acid methyl ester, 2,4,6-tri-o-methyl-d-glucose, 2,4,6-tri-o-methyl-d-galactose and the pyruvic ...19694902692
regulatory effects of substrates on a phosphotransferase from aerobacter aerogenes and modification of its activity by partial proteolysis.an enzyme isolated from aerobacter aerogenes acts both as phosphomonoesterase and phosphotransferase with glucose, glucosides, glucosamine, and n-acetyl glucosamine as acceptors. when glucose-6-phosphate is the phosphate donor, these acceptors appear to act as activators of the enzyme, while with p-nitrophenyl phosphate, alpha-glycerophosphate, fructose-1,6-diphosphate, and several other phosphate esters as donors, the same acceptors act as noncompetitive inhibitors. with p-nitrophenyl phosphate ...19695256417
biologic properties of lethal factors produced by enterobacter aerogenes. 19694905500
steady state levels of dehydrogenases and alpha- and beta-glucosidases in klebsiella aerogenes. 19695387507
experimental coliform (aerobacter aerogenes) mastitis: bacteria and host factors in virulence and resistance. 19695387936
[biosynthesis of p-aminobenzoic acid. ii. demonstration of an intermediate in a mutant of aerobacter aerogenes]. 19694391584
subcutaneous gas in diabetes mellitus due to aerobacter aerogenes infection. 19694240074
d-fructose 1-phosphate kinase and d-fructose 6-phosphate kinase from aerobacter aerogenes. a comparative study of regulatory properties. 19694242639
regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in aerobacter aerogenes and escherichia coli. 19694306838
control of respiration and metabolism in growing klebsiella aerogenes. the role of adenine nucleotides.1. a rapid-sampling technique was used to obtain perchloric acid extracts of cells growing in a chemostat culture, so that meaningful values for atp content could be obtained in spite of the fact that the turnover time for the total atp content was about 1sec. 2. for steady-state growth, it was found that, in a glucose-limited chemostat culture, the atp/adp concentration ratio was approximately constant with changes in dissolved-oxygen tensions above the critical value, but fell when the culture ...19694309671
the metabolism of d-glucarate by pseudomonas acidovorans.1. dehydratases that converted d-glucarate into 4-deoxy-5-oxoglucarate were partially purified from klebsiella aerogenes and pseudomonas acidovorans. 2. when d-glucarate was metabolized to 2,5-dioxovalerate it appeared that water and carbon dioxide were removed from 4-deoxy-5-oxoglucarate in one enzymic step: 4,5-dihydroxy-2-oxovalerate was not an intermediate in this reaction. 3. a method for the enzymic determination of d-glucarate is described.19694311826
gram-negative bacilli in burns.in a period of two years, 865 strains of gram-negative bacilli other than pseudomonas aeruginosa isolated from burns were identified by a range of tests. the commonest species were proteus mirabilis, escherichia coli, and enterobacter cloacae. many strains of klebsiella aerogenes, enterobacter aerogenes, and bacterium anitratum were also found.a large proportion of the strains were tested for sensitivity to nalidixic acid, ampicillin, kanamycin, chloramphenicol, tetracycline, and carbenicillin, ...19694312399
the isolation and characterization of a hydroxamic acid (aerobactin) formed by aerobacter aerogenes 62-i. 19694313071
an intermediate involved in the formation of 4-aminobenzoic acid from chorismic acid in aerobacter aerogenes. 196911947038
purification and some properties of the membrane-bound respiratory nitrate reductase of aerobacter aerogenes. 196911947290
d-apiose reductase from aerobacter aerogenes.a strain of aerobacter aerogenes prl-r3 has been isolated which utilizes d-apiose as its sole source of carbon. a new enzyme, d-apiose reductase, was discovered in this strain. the enzyme was not present when the strain was grown on d-glucose. d-apiose reductase catalyzes the nicotinamide adenine dinucleotide-dependent interconversion of d-apiose and d-apiitol. the enzyme is specific for d-apiose and d-apiitol, with a few possible exceptions. the k(m) for d-apiose is 0.02 m. the k(m) for d-apiit ...19704314545
structure of klebsiella aerogenes type 8 polysaccharide. 19704315134
enzymatic removal of diacetyl from beer. ii. further studies on the use of diacetyl reductase.diacetyl removal from beer was studied with whole cells and crude enzyme extracts of yeasts and bacteria. cells of streptococcus diacetilactis 18-16 destroyed diacetyl in solutions at a rate almost equal to that achieved by the addition of whole yeast cells. yeast cells impregnated in a diatomaceous earth filter bed removed all diacetyl from solutions percolated through the bed. undialyzed crude enzyme extracts from yeast cells removed diacetyl very slowly from beer at its normal ph (4.1); at a ...19704315861
susceptibility of enterobacter aerogenes and enterobacter cloacae to 19 antimicrobial agents in vitro. 19704318051
enzymatic removal of diacetyl from beer. 3. enzyme protection and regeneration of cofactor.use of diacetyl reductase, a reduced nicotinamide adenine dinucleotide (nadh)-requiring enzyme, to eliminate diacetyl off-flavor in beer was studied. the crude enzyme was extracted from aerobacter aerogenes and partially purified by ammonium sulfate precipitation or sephadex chromatography. in the semipure state, the enzyme was inactivated by lyophilization; in a crude state, the lyophilized extract remained stable for at least 4 months at - 20 c. a 50% reduction in specific activity within 5 mi ...19704318450
morphological changes in growing colonies of aerobacter (klebsiella) aerogenes. 19704392284
control of infection due to klebsiella aerogenes in a neurosurgical unit by withdrawal of all antibiotics. 19704099781
wood wa: l-ribulose 5-phosphate 4-epimerase of aerobacter aerogenes. evidence for nicotinamide adenine dinucleotide-independent 4-epimerization by the crystalline enzyme. 19704395381
biosynthesis of n-acetylanthranilic acid by aromatic auxotrophs of aerobacter aerogenes and escherichia coli. 19704923918
the isolation of o-acetylated fragments from the k antigen of escherichia coli 08:k27 (a):h by the action of phage-induced enzymes from klebsiella aerogenes. 19704918138
deletion-mapping of resistance against chlorate in klebsiella aerogenes. 19705415254
tyrosine biosynthesis in aerobacter aerogenes. purification and properties of chorismate mutase-prephenate dehydrogenase. 19705456988
studies on the subunit structure of chorismate mutase-prephenate dehydrogenase from aerobacter aerogenes. 19705456989
the oxidative degradation of benzoate and catechol by klebsiella aerogenes (aerobacter aerogenes). 19704987140
genetic determination of bacteriocin production by enterobacter aerogenes. 19704924098
experimental technique for studying aerosols of lyophilized bacteria.an experimental technique is presented for studying aerosols generated from lyophilized bacteria by using escherichia coli b, bacillus subtilis var. niger, enterobacter aerogenes, and pasteurella tularensis. an aerosol generator capable of creating fine particle aerosols of small quantities (10 mg) of lyophilized powder under controlled conditions of exposure to the atmosphere is described. the physical properties of the aerosols are investigated as to the distribution of number of aerosol parti ...19704992657
interaction of nalidixic acid with klebsiella (aerobacter) aerogenes growing in continuous culture. 19705002637
synthesis of glutamate in aerobacter aerogenes by a hitherto unknown route. 19705420057
inosine-5'-phosphate dehydrogenase of aerobacter aerogenes. studies of tertiary structure. 19705421955
[pullulanase from aerobacter aerogenes. localization of the enzyme bound to continuously grown cells. possible association with the outer membrane system of the cells]. 19705428605
undamped oscillations of pyridine nucleotide and oxygen tension in chemostat cultures of klebsiella aerogenes.klebsiella aerogenes was grown in chemostat culture with the ph controlled to +/-0.01 and temperature to +/-0.1 degrees c. the oxygen tension of the culture was regulated by changing the partial pressure of oxygen in the gas phase and recorded by means of an oxygen electrode. reduced pyridine nucleotide was monitored continuously in the culture by means of direct fluorimetry. on applying an anaerobic shock to the culture, damped oscillations in pyridine nucleotide fluorescence were obtained. fur ...19705459938
[gas chromatography in the study of bacterial metabolism. preliminary study on a strain of aerobacter aerogenes]. 19705478262
the inducible beta-galactosidase (beta-d-galactoside galactohydrolase ec 3.2.1.23) of klebsiella aerogenes ncib 9479. 19705492657
the synthesis of exopolysaccharide by klebsiella aerogenes membrane preparations and the involvement of lipid intermediates.1. membrane preparations from klebsiella aerogenes type 8 were shown to transfer glucose and galactose from their uridine diphosphate derivatives to a lipid and to polymer. the ratio of glucose to galactose transfer in both cases was 1:2. this is the same ratio in which these sugars occur in native polysaccharide. galactose transfer was dependent on prior glucosylation of the lipid. mutants were obtained lacking (a) glucosyltransferase and (b) galactosyltransferase. the transferase activities in ...19705499969
effect of enzyme concentration on the kinetics of d-lactate dehydrogenase from aerobacter aerogenes. 19705503784
action of tumour inhibitory pyrazolotriazines on klebsiella aerogenes. i. inhibition by 3,4-dimethylpyrazolo[3,2-c]-as-triazine and its antagonism by histidine. 19705507650
action of tumour inhibitory pyrazolotriazines on klebsiella aerogenes. ii. inhibition by 6-halogenoacetyl-3-methyl-4-methylenepyrazolo[3,2-c]-as-triazine and its antagonism. 19705507651
protection of aerobacter aerogenes by nonionic detergents from freezing and thawing damage. 19705510613
[methionine-deficit mutants of aerobacter aerogenes isolated under the effect of ethacidine and ultraviolet radiation]. 19705517680
the inducible neuraminidase (n-acyl-neuraminyl hydrolase ec 3.2.1.18) of klebsiella aerogenes ncib 9479. 19705531172
infection due to klebsiella aerogenes in a neurosurgical unit. 19705201011
transfer characteristics of two resistance determinants in a wild strain of klebsiella aerogenes (v9a). 19705206600
alternate pathways of d-fructose metabolism in aerobacter aerogenes. a specific d-fructokinase and its preferential role in the metabolism of sucrose. 19705440842
acetolactate decarboxylase from aerobacter aerogenes. purification and properties. 19705447429
detection of aerobacter aerogenes by labeling with radioactive phosphorus.a method for the detection of bacterial cells by labeling with (32)p has been found to be much less sensitive than was previously reported.19705418951
growth of enterobacter aerogenes in a lathyrogen-containing medium. 19705419804
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