Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| norfloxacin versus cotrimoxazole in the treatment of lower urinary tract infections. | in a randomised prospective study 61 patients with lower urinary tract infection received either 200 mg norfloxacin (33 patients) or 480 mg cotrimoxazole (28 patients) twice daily for ten days. pathogens included escherichia coli in 48 patients, proteus mirabilis in ten patients, and enterobacter cloacae, klebsiella pneumoniae, citrobacter freundii and staphylococcus saprophyticus in one patient each. the mics of norfloxacin and cotrimoxazole were less than or equal to 0.03 mg/l and less than or ... | 1983 | 6224685 |
| cefotetan: profile of in-vitro activity. | cefotetan, a novel 7 alpha-methoxy cephalosporin, was shown to be comparable to other third-generation cephalosporins when tested in vitro against 1063 gram-negative clinical isolates of 11 species. ninety per cent of all isolates of escherichia coli, klebsiella, proteus mirabilis, proteus vulgaris, proteus rettgeri, providencia stuartii, serratia marcescens and citrobacter freundii were inhibited by concentrations of cefotetan between 0.07 and 3.2 mg/l. activity against gram-positive cocci was ... | 1983 | 6601656 |
| in vitro antibacterial activity and beta-lactamase stability of e-0702, a new cephalosporin. | the in vitro activity of e-0702 was compared with the in vitro activity of cefotaxime, ceftazidime, moxalactam, and aztreonam against 600 gram-positive and gram-negative aerobic and anaerobic isolates. e-0702 had a minimal inhibitory concentration for 50% of isolates (mic50) of 25 micrograms for staphylococcus aureus, 50 micrograms for staphylococcus epidermidis, and 1.6 to 3.1 micrograms for streptococci, with streptococcus faecalis resistant. e-0702 had mic50s against escherichia coli, klebsie ... | 1983 | 6605718 |
| a new site-specific endodeoxyribonuclease from citrobacter freundii. | cfr10 i, a site-specific endonuclease from citrobacter freundii strain rfl10, was isolated. it recognizes and cleaves the family of related sequences: 5'pu decreases ccggpy to generate dna fragments with 5' tetranucleotide extensions. cfr10 i may be useful in molecular cloning experiments, especially in conjunction with other enzymes which generate the same terminal extensions. | 1983 | 6617873 |
| panophthalmitis and otitis interna in fire-bellied toads. | microbiologic and histologic studies were made of fire-bellied toads with signs of ocular and central nervous system disease. providencia alcalifaciens, citrobacter freundii, aeromonas hydrophila, and other gram-negative bacilli were isolated from the eyes and multiple tissues of ill toads. the histologic evaluations revealed severe panophthalmitis and otitis interna. | 1983 | 6643232 |
| [2 cases of osteomyelitis in acute leukemia in the induction phase of treatment]. | whereas children with acute leukemia are highly susceptible to infectious complications, the occurrence of acute osteomyelitis is extremely rare in these patients. the authors describe two such cases in children at onset of an acute lymphoblastic and of a myelomonocytic leukemia, respectively. in the former case, the clinical course has been characterized by the progressive involvement of several joints and bones. a citrobacter freundii was isolated in the synovial fluid of an involved knee. thi ... | 1983 | 6647082 |
| antibacterial activity of aztreonam: a synthetic monobactam. a comparative study with thirteen other antibiotics. | the in vitro activity of aztreoman (sq 26, 776), a new monocyclic beta-lactam antimicrobial agent, was determined against 1720 bacteria, all clinical isolates, and compared with that of thirteen beta-lactam and aminoglycoside antibiotics. aztreonam inhibited 90% of citrobacter diversus, citrobacter freundii, enterobacter agglomerans, e. coli, klebsiella pneumoniae, proteus mirabilis, proteus morganii, proteus rettgeri, proteus vulgaris and salmonella sp. by less than or equal to 0.4 micrograms m ... | 1983 | 6684718 |
| beta-lactamase inhibitory activity of iodopenicillanate and bromopenicillanate. | iodopenicillanate and bromopenicillanate were shown to be effective inhibitors of a variety of beta-lactamases. staphylococcus aureus isolates were synergistically inhibited by iodopenicillanate and bromopenicillanate combined with ampicillin. methicillin-resistant s. aureus was not synergistically inhibited. escherichia coli which possessed tem beta-lactamase activity had a reduction in ampicillin minimal inhibitory concentration, but an e. coli isolate which had chromosomal beta-lactamase and ... | 1983 | 6299186 |
| unusual mode of inhibition of citrobacter freundii beta-lactamases by ceftriaxone. | several citrobacter freundii beta-lactamases, discernible by their substrate profiles and sensitivities to inhibition by newer cephalosporins, were inhibited by ceftriaxone in an unusual manner. inhibition was noncompetitive at low concentrations of cephaloridine but became competitive at concentrations above 600 mum, which is close to the k(m) (570 mum) for cephaloridine. | 1983 | 6301366 |
| [microbiological studies with the new penicillin brl 17421 (temocillin)]. | in vitro evaluation of brl 17421 (temocillin), a new penicillin. the in vitro antibacterial activity of brl 17421 (temocillin), a new penicillin, was determined in quantitative serial broth dilution tests and was compared to that of mezlocillin, piperacillin, cefazolin and cefotaxime against 751 clinical isolates of the enterobacteriaceae family. in addition, the sensitivity of 211 mezlocillin-resistant gram-negative rods to brl 17421 was also determined. temocillin exhibited a high level of ant ... | 1983 | 6302007 |
| cloning and expression of the gene(s) for cephalosporinase production of citrobacter freundii. | the chromosomal gene(s) for cephalosporinase production of citrobacter freundii gn346 has been cloned into vector plasmid pmk1, initially as a 7.3 kb ecori fragment. from the substrate profile and the response to anti-gn346 csase serum of the enzyme produced, it was confirmed that the hybrid plasmid (pty71) carries the relevant chromosomal cephalosporinase gene from c. freundii gn346. a restriction endonuclease cleavage map of cloned ecori fragments was constructed, and the structural gene of th ... | 1983 | 6304475 |
| in vitro activity and beta-lactamase stability of cefodizime, an aminothiazolyl iminomethoxy cephalosporin. | cefodizime, an iminomethoxy aminothiazolyl cephalosporin similar to moxalactam and ceftazidime, was less active (minimal inhibitory concentration, 1.6 to 12 micrograms) than cefazolin or cefotaxime against staphylococcus aureus and staphylococcus epidermidis. it inhibited haemophilus and neisseria spp. at less than 0.5 microgram/ml. it did not inhibit methicillin-resistant staphylococci, enterococci, or listeria spp. and was 8- to 32-fold less active than cefotaxime, moxalactam, or ceftazidime a ... | 1983 | 6311090 |
| [in vitro bacteriostatic activity of cefmenoxime (sce 1365), cefotaxime and moxalactam]. | the in vitro activity of cefmenoxime (sce 1365), a new cephalosporin derivate was compared with two other "third generation" cephalosporins: cefotaxime and moxalactam. cefmenoxime as cefotaxime and moxalactam were very active against 305 cephalosporinase-producing and cephalosporinase-non-producing enterobacteriaceae. cefmenoxime was the most active against serratia marcescens, citrobacter freundii, morganella morganii, salmonella, shigella and yersinia enterocolitica with a mean mic at least tw ... | 1983 | 6312395 |
| kinetic studies on inactivation of citrobacter freundii cephalosporinase by sulbactam. | the inactivation kinetics for inhibition by sulbactam (cp45,899) of citrobacter freundii gn346 cephalosporinase were studied in detail and compared with those of type ib penicillinase or tem-2 beta-lactamase mediated by r plasmid rgn823. the rate constant for progressive inactivation of the cephalosporinase was significantly larger than that measured with the penicillinase. the number of sulbactam molecules required to cause complete inactivation of one cephalosporinase molecule (turnover number ... | 1983 | 6312873 |
| [distribution of specific endodeoxyribonucleases in different strains of citrobacter freundii]. | 1983 | 6313311 | |
| survey, purification, and properties of sugar phosphate phosphohydrolase among microorganisms. | sugar phosphate phosphohydrolase was purified approximately 500- to 600-fold to apparent homogeneity from escherichia coli b, escherichia coli c, escherichia coli var. communior, escherichia acidilactici, enterobacter aerogenes, neisseria meningitidis, and saccharomyces cereviseae. the molecular weights of the enzyme as estimated by gel filtration ranged from 97 x 10(3) to 101 x 10(3). the enzyme was composed of two subunits with the same molecular weight which ranged from 50 x 10(3) to 52 x 10( ... | 1983 | 6322944 |
| differential toxicity of inhaled gram-negative bacteria. | the toxicity by inhalation of various gram-negative bacteria, isolated from settings associated with inhalation disease, was studied by a variety of means. these microorganisms were not equally toxic. citrobacter freundii aerosol challenges of rabbits provoked significant (up to fivefold) increases in plasma haptoglobin 24 to 48 h after inhalation. other strains tested failed to provoke such statistically consistent increases. measurements of c-reactive protein in these same animals did not lead ... | 1983 | 6339400 |
| [the antibiotic agent azthreonam: studies on plasmid-dependent resistance formation]. | two mechanisms can be assumed that are responsible for the stability of azthreonam against the attack of beta-lactamases: first, azthreonam lacks binding to the enzyme protein and second, rate of hydrolysis is extremely low, resulting even in inhibition of enzyme activity. enzyme kinetics were studied from highly purified enzymes: azthreonam did not bind to the tem-1 enzyme, whereas the compound revealed a time-dependent inhibition of the chromosomally mediated enterobacter cloacae enzyme. with ... | 1983 | 6341212 |
| phenomenon of resistance to augmentin associated with sensitivity to ampicillin: occurrence and explanation. | ampicillin was found to be some tenfold more active than amoxycillin against enterobacter cloacae. this finding explains the observation that some ent cloacae strains are sensitive to ampicillin in the disc test but resistant to augmentin. ampicillin was also found to be more active than amoxycillin against citrobacter freundii and serratia marcescens. in view of these findings, the practice of using ampicillin discs to predict sensitivity to amoxycillin should be reconsidered. the use of both a ... | 1983 | 6343436 |
| incompatibility group k plasmids in bacteria isolated from a urinary tract infection. | citrobacter freundii and klebsiella pneumoniae were concurrently isolated from a patient with a urinary tract infection. transferable drug resistant plasmids were isolated from both strains, pms434 and pms435. these plasmids belonged to incompatibility group k and both carried genes governing resistance to various aminoglycoside antibiotics, i.e., kanamycin, gentamicin c complex, streptomycin, and 3',4'-dideoxykanamycin b, in addition to those governing resistance to sulfanilamide and ampicillin ... | 1983 | 6346022 |
| synthesis of plasmid-coded heat-labile enterotoxin in wild-type and hypertoxinogenic strains of escherichia coli and in other genera of enterobacteriaceae. | the effect of host determinants on expression of plasmid-coded heat-labile enterotoxin (lt) was examined. a collection of lt plasmids was introduced into isogenic strains of escherichia coli k-12 strains containing the wild type or hypertoxinogenic (htx-2) allele. for each plasmid tested, production of lt increased by approximately 1.5- to 3-fold in the host containing htx-2, indicating that the htx-2 allele affects a regulatory function for lt production that is common to many different enterot ... | 1983 | 6350177 |
| comparison of the overlapping frd and ampc operons of escherichia coli with the corresponding dna sequences in other gram-negative bacteria. | specific dna probes from escherichia coli k-12 were used to analyze the sequence divergence of the frd and ampc operons in various species of gram-negative bacteria. these operons code for the fumarate reductase complex and the chromosomal beta-lactamase, respectively. we demonstrate that the two operons show the same general pattern of divergence, although the frd operon is considerably more conserved than is the ampc operon. the major exception is salmonella typhimurium lt2, which shows a stro ... | 1983 | 6350266 |
| evaluation of the updated ms-2 bacterial identification system in comparison with the api 20e system. | the recently updated ms-2 bacterial identification system software (abbott laboratories, diagnostic division, irving, tex.) was compared with the original ms-2 bacterial identification software and the api 20e, using 968 strains of enterobacteriaceae. the updated ms-2 software correctly identified 94.4% of the isolates tested. api 20e and the original ms-2 software correctly identified 91 and 85.3% of the strains, respectively. ms-2 responses were considered to be equivocal (needing additional t ... | 1983 | 6350342 |
| efficacy of brl 25000 against serratia marcescens, enterobacter cloacae, and citrobacter freundii in urinary tract infections. | synergism between amoxicillin and clavulanic acid was not expected against cephalosporinase-producing bacterial strains because clavulanic acid has little inhibitory action on cephalosporinases. however, in a clinical trial of brl 25000 (amoxicillin-clavulanic acid), excellent results were obtained in complicated urinary tract infections caused by serratia marcescens, enterobacter cloacae, and citrobacter freundii strains which produced cephalosporinase and were highly resistant to amoxicillin a ... | 1983 | 6357078 |
| influence of methoxy-substitution of beta-lactam compounds on the interaction with various beta-lactamases. | the interaction of 6 alpha-(temocillin) and 7 alpha-methoxy substituted (cefoxitin) beta-lactam compounds with various beta-lactamases was studied employing enzyme kinetics and compared to that of unsubstituted compounds. both chromosomally mediated enzymes from enterobacter cloacae and citrobacter freundii were competitively inhibited by the methoxy-substituted compounds. higher concentrations of cefoxitin caused a competitive inhibition of the plasmid-mediated tem-1 enzyme, whereas temocillin ... | 1983 | 6362278 |
| investigations on beta-lactamase stability of recently developed beta-lactam compounds: study of enzyme kinetics. | the plasmid-mediated tem-1 enzyme (e. coli k12 r6k) and chromosomally mediated enzymes from enterobacter cloacae (iep 8.5) and citrobacter freundii (iep 9.5) were highly purified. enzyme kinetics were studied with various therapeutic compounds as substrates and lamoxactam, azthreonam, and n-formimidoyl thienamycin as inhibitors. lamoxactam and azthreonam failed to inhibit the tem-1 enzyme, whereas n-formimidoyl thienamycin was a competitive inhibitor. ki was 5 mumol/l--thus corresponding to km-- ... | 1983 | 6372325 |
| novel carbapenem derivative sf2103a: studies on the mode of beta-lactamase inactivation. | a novel carbapenem, sf2103a, is a strong inhibitor of various types of beta-lactamase. equimolar concentrations of sf2103a completely inactivated the cephalosporinases of proteus vulgaris and citrobacter freundii and type ib and type ii penicillinases mediated by r plasmids in a progressive manner. the inactivation of the two penicillinases and p. vulgaris cephalosporinase was apparently irreversible; however, when the inactivated enzymes were separated from excess sf2103a by gel filtration, the ... | 1984 | 6372682 |
| resistance types in citrobacter freundii. occurrence and resistance to ampicillin, carbenicillin, cephalothin and mecillinam. transfer of ampicillin resistance. | the resistance types of citrobacter freundii were investigated. the strains were divided into three groups according to susceptibility to penicillin derivatives. group 1, comprising the ampicillin-carbenicillin sensitive strains (a-s/ca-s), constituted 22%. group 2 (ampicillin-resistant (a-r)/ca-s) constituted 45% and group 3 (a-r/ca-r) 32%. two strains (1%) were recorded as a-s/ca-r. group 3 contained a higher proportion of multi-resistant strains than groups 1 and 2. the log2ic50 with ampicill ... | 1984 | 6375251 |
| [enzyme activity of the formate hydrogenlyase complex in citrobacter freundii]. | citrobacter freundii 62 can grow in the absence of oxygen in media containing glucose, peptone, fumarate or malate. when the medium contained fumarate or malate, the culture could grow under anaerobic conditions only in the presence of molecular hydrogen, formate or nitrate. the highest activity of formatehydrogenlyase and hydrogenase was found when c. freundii grew in a medium with glucose and formate. the activity was lower in media with other organic substrates, particularly, in the absence o ... | 1984 | 6377026 |
| intergeneric homology of the spec gene encoding biosynthetic ornithine decarboxylase in escherichia coli. | a 32p-labeled fragment of dna containing the spec gene, which encodes the biosynthetic enzyme ornithine decarboxylase of escherichia coli, was used as a hybridization probe for homologous sequences in the genomes of gram-negative and gram-positive bacteria. the spec probe detected homologous sequences in the dna of only four members of the enterobacteriaceae (citrobacter freundii, salmonella typhimurium, klebsiella pneumoniae, and enterobacter aerogenes); no homology was detected with the dna of ... | 1984 | 6384181 |
| cephalosporin antibiotics as applied in surgery of bones and joints. | new cephalosporin antibiotics will continue to be discovered as old forms are found to lose effectiveness. cephalosporins currently account for a major part of any hospital pharmacy budget. they are often used inappropriately in treatment of both community and hospital infections. cephalosporins should be used when penicillins are not effective and for particular infections. a major use of cephalosporins is in prophylaxis; cephalosporin antibiotics will not prevent infections caused by organisms ... | 1984 | 6386261 |
| incidence of trimethoprim-sulfamethoxazole-resistant enterobacteriaceae among transplant recipients. | of 114 recipients of pancreatic, renal, and bone marrow transplants who were given trimethoprim-sulfamethoxazole (tmp-smz) for antimicrobial prophylaxis, 44 (39%) had a total of 52 fecal isolates of tmp-smz-resistant gram-negative bacilli. in most of these 44 patients, the resistant isolate was found at a concentration of greater than or equal to 10(6) organisms/ml of feces. escherichia coli was the most frequent of the isolates, and citrobacter freundii was the next most frequent. eight of the ... | 1984 | 6387000 |
| evaluation of rosco diagnostic beta-glucuronidase tablets in the identification of urinary isolates of escherichia coli. | rosco diagnostic beta-glucuronidase tablets have been evaluated as a method for the identification of urinary isolates of escherichia coli. results were compared with those from traditional biochemical testing. a total of 539 isolates were employed, representing a variety of gram-negative species. reproducibility testing was also performed. after 4h incubation, 86% of e. coli isolates (both lactose-positive and lactose-negative) gave a positive reaction. some salmonella, many shigella and one ci ... | 1984 | 6393708 |
| septicemia due to a lactose-fermenting salmonella misidentified as citrobacter freundii. | 1984 | 6394546 | |
| monoclonal antibody-based enzyme-linked immunosorbent assays for identification and serotyping of vibrio cholerae o1. | monoclonal antibodies directed against o-specific antigens of vibrio cholerae o1 lipopolysaccharide were used in two different enzyme-linked immunosorbent assays (elisas), designed for identification and serotyping of v. cholerae o1. in the sandwich elisa, a monoclonal antibody against the group-specific antigen was used as capture antibody, whereas peroxidase-conjugated monoclonal antibodies directed against group- and type-specific antigens were used as the second antibodies. monoclonal antibo ... | 1984 | 6394621 |
| use of the autobac idx system for rapid identification of enterobacteriaceae and nonfermentative gram-negative bacilli. | the autobac idx system was evaluated for its ability to accurately identify 290 gram-negative bacilli from 18 different genera. excluding isolates with a low identification probability, the overall sensitivity of the system was found to be 95.8%. late lactose-fermenting escherichia coli, citrobacter freundii, and proteus mirabilis accounted for over 90% of the misidentifications. the autobac idx system offers a rapid and reliable method for the identification of gram-negative bacilli. | 1984 | 6365967 |
| [specificity of new restrictases and methylases. unusual modification of cytosine at position 4]. | fourteen restriction endonucleases and 4 methylases were isolated and purified from 14 strains of citrobacter freundii and escherichia coli, which were isolated from natural sources. to determine the nucleotide sequence recognized by the endonucleases a comparison of dna cleavage patterns, the evaluation of the cleavage frequency of some dna with known recognition sequences and mapping was used. it was determined that cfr101 is a new enzyme recognizing 5'puccggpy. other restriction enzymes isola ... | 1984 | 6323968 |
| comparative in vitro activity and beta-lactamase stability of fr 17027, a new orally active cephalosporin. | fr 17027, a new orally absorbed cephalosporin ester, inhibited group a and b streptococci and streptococcus pneumoniae at less than or equal to 0.1 micrograms/ml, which is similar to the inhibition concentration of amoxicillin and cefaclor, and was more active than cephalexin. it was less active (mic, 25 micrograms/ml) against staphylococci than was cephalexin, and it did not inhibit streptococcus faecalis or listeria monocytogenes. fr 17027 inhibited beta-lactamase-producing isolates of neisser ... | 1984 | 6333207 |
| synergism of ps-5 with penicillins and cephalosporins in antimicrobial activity against beta-lactam-resistant gram-negative microorganisms. | the in vitro synergism of ps-5 combined with various penicillins and cephalosporins in antimicrobial activity was examined in detail against beta-lactam-resistant gram-negative bacteria. ps-5 showed a highly significant synergism in antimicrobial action against escherichia coli rgn238 in combination with penicillins; and against proteus vulgaris gn76 and serratia marcescens t55 in combination with cephalosporins. it was moderately synergistic against citrobacter freundii gn346, enterobacter cloa ... | 1984 | 6334679 |
| selection of resistant mutants of citrobacter freundii by second and third-generation cephalosporins and imipenem. | using a single-step selection procedure, resistant mutants could be obtained from three clinical isolates of citrobacter freundii with two second-generation and four third-generation cephalosporins but not with imipenem. all mutants showed a drastically increased beta-lactamase activity and were cross-resistant to all the cephalosporins examined. combinations of cloxacillin with the cephalosporins were markedly synergistic, suggesting the principal role of the cephalosporinase in the resistance ... | 1984 | 6335134 |
| beta-lactamase production in enterobacter cloacae and citrobacter freundii. | the beta-lactamase activity with ampicillin (a) and cephalothin (ce) as substrate was examined in 28 strains of ent. cloacae and 24 strains of c. freundii by a micro-iodometric-(mia) and an ultraviolet assay (uva). carbenicillin resistance (ca-r) was present in 32 strains (16 of each species). all ca-resistant (ca-r) strains had elevated amounts of beta-lactamase. the conditions were very similar in the two species. the ca-r strains could be divided into two subgroups. one group had very high am ... | 1984 | 6335817 |
| [immobilization of citrobacter freundii cells with tyrosine phenol-lyase activity by entrapment in natural gels]. | a comparative study on immobilization of citrobacter freundii cells by entrapment in carrageenan, agar, agar-agar, and gelatin gels (5, 10, and 15%) was carried out. gelatin gels were treated with glutaraldehyde to make them more rigid. as a result, the tyrosine phenol-lyase activity of these samples was less than that of free cells (about 40%). the yield of tp-lyase activity was 40--60% when cells were immobilized in 5% and 7% agar and agar-agar gels. the cells entrapped in carrageenan gels, th ... | 1984 | 6701163 |
| the distribution of repeating [gal beta 1,4glcnac beta 1,3] sequences in asparagine-linked oligosaccharides of the mouse lymphoma cell lines bw5147 and phar 2.1. | the occurrence and distribution of the repeating disaccharide [gal beta 1,4glcnac beta 1,3] in the different types of asn-linked oligosaccharides in mouse lymphoma bw5147 cells have been studied. glycopeptides were prepared from cells grown in medium containing [6-3h]galactose, and the bi-, tri-, and tetraantennary asn-linked oligosaccharides were fractionated by serial lectin affinity chromatography on concanavalin a-sepharose, pea lectin -sepharose, leukoagglutinating phytohemagglutinin-agaros ... | 1984 | 6725252 |
| development of resistance to cephalosporins in clinical strains of citrobacter spp. | the predominant beta-lactam antibiogram of citrobacter freundii resembles that of enterobacter cloacae in demonstrating resistance to cephalothin and cefoxitin with susceptibility to the newer cephalosporins. four representative strains of c. freundii were reversibly induced to high-level beta-lactamase production by cefoxitin, and mutants with stable, high-level production were selected with cefamandole. the mutants were resistant to several second- and third-generation cephalosporins. comparis ... | 1984 | 6610388 |
| study of the in vitro activity of norfloxacin and other drugs on amoxicillin-resistant uropathogenic isolates. | the in vitro activity of a quinolinecarboxylic acid compound, norfloxacin, was compared with those of amikacin, carbenicillin, cefazolin, cefoxitin, gentamicin, nalidixic acid, and oxolinic acid against 243 gram-positive and gram-negative amoxicillin-resistant (mic 800 greater than or equal to micrograms/ml) uropathogenic isolates. norfloxacin showed remarkable activity against the majority of the bacteria tested. ninety percent of strains of staphylococcus aureus, streptococcus faecalis, citrob ... | 1984 | 6241505 |
| human placental (fetal) fibronectin: increased glycosylation and higher protease resistance than plasma fibronectin. presence of polylactosamine glycopeptides and properties of a 44-kilodalton chymotryptic collagen-binding domain: difference from human plasma fibronectin. | human placental fibronectin was isolated from fresh term placenta by urea extraction and purified by gelatin affinity chromatography. a 44-kda chymotryptic fragment, also purified by gelatin affinity chromatography, gave a broad, diffuse band on polyacrylamide gel electrophoresis, whereas the analogous 43-kda fragment from human plasma fibronectin migrated as a defined, narrow band. upon extended treatment with endo-beta-galactosidase from escherichia freundii, the 44-kda chymotryptic gelatin-bi ... | 1984 | 6423638 |
| endo-beta-d-galactosidases of bacteroides fragilis and escherichia freundii hydrolyze linear but not branched oligosaccharide domains of glycolipids of the neolacto series. | the specificities of the endo-beta-galactosidases of bacteroides fragilis and escherichia freundii towards linear and branched oligosaccharides of the lacto-n-glycosyl series were investigated using as substrates glycolipids containing (a) linear neolactotetra - or hexaosyl sequences, (b) branched biantennary neolactooctaosyl sequences, and (c) triantennary neolactononaor dodecaglycosyl sequences. glycolipid and oligosaccharide hydrolysis products were identified by tlc and/or paper chromatograp ... | 1984 | 6427218 |
| [endo-beta-galactosidase from flavobacterium keratolyticus and escherichia freundii]. | 1984 | 6431498 | |
| [in vitro antibacterial activity of ciprofloxacin against uropathogenic organisms]. | ciprofloxacin (bay 09867) is a new quinoline derivative. its activity against 537 urinary bacterial isolates was compared with those of ampicillin, amikacin, carbenicillin, cefazolin, cefotaxime, cefoxitin, ceftazidime, nalidixic and oxolinic acids, norfloxacin, trimethoprim, and other antimicrobial agents, when appropriate. it inhibited 90% citrobacter freundii, enterobacter spp., e, coli, klebsiella spp., proteus spp., and serratia spp. at less than or equal to 0.78 microgram/ml. the majority ... | 1984 | 6440583 |
| growth of clinical isolates in the cold. | common clinical isolates were tested to determine their ability to multiply after 7 days at 4 degrees c in trypticase soy broth. 34% of the 214 isolates grew in the cold. citrobacter freundii, group d enterococci, klebsiella oxytoca, proteus vulgaris, providencia rettgeri and serratia marcescens grew best of all. the importance of growth in the cold is discussed. | 1984 | 6472199 |
| microbial aspects of the cockroach hindgut. | the cockroach hindgut has a complex, active microbiota, a large portion of which is associated with the chitinous gut wall. it provides a different environment from that of termites and other insects which are dependent on their hindgut microbiota for the digestion of cellulose. the ph of the midgut of eublaberus posticus was not as high as it is in insects with a primarily cellulolytic nutrition. the hindgut of e. posticus was highly methanogenic, normal adults producing typically 10-25 mumol o ... | 1984 | 6477029 |
| [study of lincomycin concentrations in hepatocystic duct]. | penetration of lincomycin (lcm) in choledochal and cholecystic bile as well as in the gallbladder tissue and liver tissue was investigated together with bacteria detectable in the bile in order to evaluate basically usefulness of this antibiotic in the treatment of infections of the hepatocystic duct. intravenous drip infusion of lcm 1.5 g (in 500 ml of 5% glucose solution) over 1.5--2 hours resulted in mean drug concentrations of 33.9 and 10.1 micrograms/ml in serum at 2 and 4 hours post start ... | 1984 | 6502929 |
| cefoperazone in the treatment of postsurgical wound infection, sepsis, and abscess of the spinal cord and brain. | cefoperazone was used in the treatment of 23 cases of serious bacterial infection in 20 patients. ten postoperative scalp wound infections, five infections at the site of tracheostomy, four cases of extradural spinal cord abscess, three cases of sepsis, and one abscess of the cerebellopontine angle were treated with cefoperazone (1 to 2 gm bid, usually for seven to 27 days). there were excellent or good clinical responses in 87% (20/23) of the cases (14 of the 15 postsurgical wound infections, t ... | 1984 | 6509463 |
| in vitro and in vivo antibacterial properties of fk 027, a new orally active cephem antibiotic. | fk 027 was more active than cefaclor, cephalexin, and amoxicillin against stock strains of a wide variety of gram-negative bacteria, including such opportunistic pathogens as citrobacter and enterobacter species and serratia marcescens. fk 027 was significantly more active than the three reference drugs against clinical isolates of escherichia coli, klebsiella pneumoniae, indole-positive and -negative proteus species, providencia species, haemophilus influenzae, and neisseria gonorrhoeae. it was ... | 1984 | 6561017 |
| enoxacin: worldwide in-vitro activity against 22451 clinical isolates. | a worldwide multicentre study involving 31 centres in eight countries was conducted to compare the in-vitro activity of enoxacin with that of other currently available antibiotics. enoxacin was active against virtually all of the species of enterobacteriaceae tested (mic90 less than or equal to 0.25-2 mg/l). it was also effective at inhibiting pseudomonas aeruginosa (mic90 = 2 mg/l; n = 3540), staphylococcus aureus and epidermidis (mic90 = 2 mg/l; n = 2635 and 837 resp.), acinetobacter calcoacet ... | 1984 | 6594322 |
| specific identification of salmonella serogroup e antigen o3 by immunofluorescence and coagglutination with antiserum elicited by a synthetic trisaccharide-bovine serum albumin glycoconjugate. | antiserum specific for salmonella o3 antigen was raised by immunization of rabbits with an artificial glycoconjugate consisting of the synthetic trisaccharide beta-d- manp (1----4)-alpha-l- rhap (1----3)-alpha-d-galp covalently linked to bovine serum albumin (beta- mrg -bsa). enzyme immunoassays showed that only lipopolysaccharides extracted from salmonella serogroup e (o3 antigen-containing) bacteria bound the antiserum. the usefulness of the beta- mrg -bsa antiserum for rapid and accurate iden ... | 1984 | 6203930 |
| correction of the nucleotide sequence of the citrobacter freundii tryptophan operon regulatory region. | we present a correction of the previously reported nucleotide sequence of the citrobacter freundii trp operon regulatory region. the original sequence analyses were performed with a plasmid designated pcf2. we repeated the cloning of the trp regulatory region of c. freundii and concluded from the determined sequence that a dna rearrangement had occurred within the leader region of the cloned trp dna of pcf2. the correct sequence is homologous to the escherichia coli sequence. | 1984 | 6090386 |
| in vivo antibacterial activity of cefodizime, a new cephalosporin antibiotic. | the in vivo activity of cefodizime (hr 221) was compared with that of cefotaxime (ctx), cefmenoxime, latamoxef, cefazolin and cefmetazole (cmz). the protective effects of hr 221 on experimental infections in mice caused by staphylococcus aureus smith, escherichia coli c-11, proteus vulgaris gn-76 and serratia marcescens no. 2 were directly related to its in vitro activity against these strains. in contrast, the compound showed the smallest ed50 values, among the 5 antibiotics tested (not includi ... | 1984 | 6092740 |
| treatment of 160 cases of acute bacterial meningitis with cefotaxime. | one hundred and sixty cases of acute bacterial meningitis were treated with cefotaxime. patients were between 9 days and 79 years old: 7 new borns, 37 infants, 43 children, 19 adolescents and 54 adults. fifty-eight patients (36%) were in coma when admitted. aetiology was determined in 110 patients (68.8%): neisseria meningitidis in 42, streptococcus pneumoniae in 36, haemophilus influenzae in 16, salmonella spp. in 7, staphylococcus aureus in 2, enterobacter spp. in 2 and haemophilus parainfluen ... | 1984 | 6094440 |
| single-dose cefotaxime in the treatment of urinary tract infections in children: a randomized clinical trial. | thirty-seven patients with a median age of 5 years with symptomatic lower or upper urinary tract infection, documented by a clean-catch midstream urine culture and sediment examination, completed a randomized study comparing the effectiveness of a single dose of cefotaxime with 10-day treatment with an antibiotic chosen by in-vitro sensitivities. eighteen patients were randomly assigned to the cefotaxime group (50 mg/kg/im route) and 19 patients were placed in the 10-day therapy group. in the ce ... | 1984 | 6094457 |
| cefmenoxime: clinical evaluation. | cefmenoxime was evaluated in an open trial consisting of 41 patients. forty infections in 36 patients could be evaluated. thirteen patients had pyelonephritis due to escherichia coli (two bacteremic), pseudomonas aeruginosa, klebsiella pneumoniae, or streptococcus faecalis; all improved and 12 of 13 were clinically cured, but one relapse (s. faecalis) occurred at two weeks. six patients with cystitis due to e. coli, citrobacter freundii, serratia marcescens, p. aeruginosa, or s. faecalis all imp ... | 1984 | 6097126 |
| endocarditis due to citrobacter freundii. | the etiologic association of acute bacterial endocarditis with the citrobacter species has been rare, although it is one of the opportunistic organisms that afflicts the elderly, neonates, the debilitated and immunocompromised. we report a case of endocarditis due to citrobacter freundii. | 1984 | 15227087 |
| efficacy of bicozamycin in preventing traveler's diarrhea. | bicozamycin was compared with a placebo in a prospective, randomized, double-blind study of the prevention of acute diarrhea among 30 american travelers newly arrived in guadalajara, mexico. none of the 11 subjects given bicozamycin orally for 3 wk at a dosage of 500 mg four times a day developed diarrhea as compared with an incidence of 53% diarrhea (10 of 19 subjects) in the placebo group (p = 0.003). bicozamycin was well tolerated. studies of changes in predominant aerobic fecal flora among t ... | 1985 | 3965336 |
| ureolytic citrobacter freundii infection of the urine as a cause of dissolution of cystine renal calculi. | we report a case of cystinuria with staghorn renal lithiasis in a solitary right kidney and chronic renal failure. right nephropyelolithotomy was performed and although 29 renal calculi were extracted many stones remained in situ. a permanent nephrostomy was left in the kidney. several months later the urine was infected chronically with a ureolytic citrobacter freundii bacteria and urinary ph oscillated between 8.0 and 9.2. spontaneous dissolution of the cystine calculi was observed and many ti ... | 1985 | 3973994 |
| the emergence of resistance and the therapy of septicaemia. | there were 658 positive blood cultures in st. thomas' hospital during 1982-1984, from which 301 enterobacteriaceae were isolated. most were of species that do not usually produce inducible class i beta-lactamases but 39 of the isolates were of species that do. many of these strains were resistant to the older cephalosporins but resistance to cefuroxime was uncommon and resistance to ceftazidime or cefotaxime was confined to five isolates of enterobacter cloacae (four of which were from patients ... | 1985 | 3986947 |
| embryonal lactosaminoglycan. the structure of branched lactosaminoglycans with novel disialosyl (sialyl alpha 2----9 sialyl) terminals isolated from pa1 human embryonal carcinoma cells. | lactosaminoglycan glycopeptides were isolated from human pa1 embryonal carcinoma cells and their structures were elucidated. the glycopeptides were digested by escherichia freundii endo-beta-galactosidase before and after the modifications by exoglycosidases. the core glycopeptides and oligosaccharides thus obtained and the intact glycopeptides were analyzed by methylation, fast atom bombardment-mass spectrometry, and high-performance liquid chromatography. based on these experiments, the struct ... | 1985 | 3997842 |
| [fatal citrobacter freundii bronchopneumonia acquired in the community in an uncompromised patient]. | 1985 | 4001494 | |
| in vitro comparative antimicrobial activity of cefpimizole against clinical isolates from five medical centers. | cefpimizole was compared with cefoperazone and cefotaxime against 6,599 clinical bacterial isolates from five medical centers. cefoperazone and cefotaxime were both more active and provided a greater spectrum of antimicrobial coverage than cefpimizole. some of the cefpimizole minimum concentrations inhibiting 50% of tested strains were as follows: citrobacter freundii and enterobacter cloacae, 16 micrograms/ml; escherichia coli and klebsiella pneumoniae, 2.0 micrograms/ml; proteus mirabilis, 1.0 ... | 1985 | 4026275 |
| an unusual mixed infection in a diabetic patient: citrobacter freundii and peptococcus magnus. | a case of an unusual mixed infection of the foot involving citrobacter freundii and peptococcus magnus is presented. characteristics and treatment of infections caused by these organisms are reviewed. the possible effects of an infection of this type in a compromised patient are also presented. | 1985 | 4045113 |
| population analysis of susceptibility to cefotaxime in enterobacteriaceae. | population analysis of susceptibility to cefotaxime (ctx) in eight species of enterobacteriaceae was carried out. one strain of each species was examined. the strains represented their individual species as regards natural susceptibility to ampicillin (a) and carbenicillin (ca). cephalothinase activity of the parental strains and strains selected on the plates with the highest concentration that allowed growth was determined by an ultraviolet assay. the populations of the a-susceptible/ca-suscep ... | 1985 | 4083013 |
| holding effects on coliform enumeration in drinking water samples. | standard procedures for analyzing drinking water stress the need to adhere to the time and temperature conditions recommended for holding samples collected for microbiological testing. the national drinking water laboratory certification program requires compliance with these holding limits, but some investigators have reported difficulties in meeting them. research was conducted by standard analytical methods to determine if changes occurred when samples were held at 5 and 22 degrees c and anal ... | 1985 | 4083877 |
| selective detection and enumeration of fecal coliforms in water by potentiometric measurement of lipoic acid reduction. | water samples of various origins were inoculated into a specific coliform-selective lactose broth provided with lipoic (thioctic) acid, and the time evolution of the redox potential of the cultures was monitored during incubation at 41 degrees c by use of gold versus reference electrodes. positive potential-time responses, i.e., 100-mv potential shifts recorded within 20 h of inoculation, were related to the initial number of fecal coliforms in the broth determined by control enumeration techniq ... | 1985 | 4091553 |
| [clinical and experimental studies on the pathogenicity of clinical isolated citrobacter freundii]. | clinical and fundamental studies were performed using citrobacter freundii isolated from urine specimens of in- and out-patients between january 1980 and july 1981. the c. freundii (greater than 10(4)/ml) isolated from urine was closely related to chronic complicated urinary tract infection. mics of pyridonecarbonic acids (enx, oflx and nflx) and cmx against c. freundii were low. pyridonecarbonic acids (enx, oflx and nflx) and cmx showed good therapeutic effects against the experimental ascendin ... | 1985 | 3851621 |
| in vitro antibacterial activity of bmy-28142, a new extended-spectrum cephalosporin. | the in vitro activity of bmy-28142 was compared with that of cefotaxime, ceftazidime, moxalactam, and imipenem against 639 clinical isolates and a number of in vitro-selected resistant mutants. bmy-28142 was the most potent compound against the members of the family enterobacteriaceae with a mic for 90% of the strains of 0.12 micrograms/ml. the activity against pseudomonas aeruginosa was comparable to that of ceftazidime and imipenem. strains of staphylococci were moderately susceptible to bmy-2 ... | 1985 | 3859244 |
| in-vitro activity of ofloxacin, a quinolone carboxylic acid compared to other quinolones and other antimicrobial agents. | ofloxacin is a new quinolone carboxylic acid compound. its activity against 900 bacterial isolates was determined. it inhibited 90% of escherichia coli, klebsiella sp., aeromonas hydrophila, salmonella spp., shigella spp., citrobacter spp., enterobacter spp., morganella morganii, proteus mirabilis, yersinia enterocolitica at less than or equal to 0.8 mg/l. branhamella catarrhalis, haemophilus sp., neisseria sp. were inhibited by less than or equal to 0.1 mg/l. pseudomonas aeruginosa and other ps ... | 1985 | 3865923 |
| conversion of cloxacillin into a progressive inhibitor of beta-lactamases by sulfonation and its activity against various types of these enzymes. | on the assumption of the theory that the sulfone of a penam derivative should effectively act as a suicide substrate against beta-lactamases to which the parent compound is a poor substrate, the action of cloxacillin sulfone on four different types of beta-lactamases was studied. as we expected, cloxacillin sulfone showed strong mechanism-based irreversible inactivation against type ib penicillinase and proteus vulgaris cephalosporinase whereas it showed no progressive inactivation against cloxa ... | 1985 | 3871761 |
| evidence for nonspecific induction of beta-lactamase in overproducing variants of enterobacter cloacae and citrobacter freundii. | induction of chromosomally mediated beta-lactamases was studied in clinical isolates of enterobacter cloacae and citrobacter freundii. whereas isolates resistant to ampicillin and cefoxitin exhibited an inducible enzyme, those sensitive to both agents did not. cefoxitin and above all imipenem proved the most efficacious enzyme inducers. variants among these inducible isolates which produced large amounts of beta-lactamase could be selected in the presence of cefamandole. in each of these selecte ... | 1985 | 3872794 |
| in vitro and in vivo activities of dn-9550, a new broad-spectrum cephalosporin. | dn-9550 [(6r, 7r)-7-[(z)-2-(2-aminothiazol-4-yl)-2-(1h-imidazol-4-yl) methoxyiminoacetamido]-3-[(1-pyridinio)methyl]-8-oxo-5-thia -1-azabicyclo methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate hydrochloride] is a new semisynthetic cephalosporin with a broad spectrum of antibacterial activity against gram-positive and gram-negative bacteria. the activity of dn-9550 against most species of the family enterobacteriaceae was roughly comparable to that of ceftazidime, slightly lower th ... | 1985 | 3873898 |
| covalent binding of moxalactam to cephalosporinase of citrobacter freundii. | the inhibition of citrobacter freundii cephalosporinase activity by moxalactam is shown to be due to the formation of a transiently stable covalent complex, probably acyl enzyme. the covalent complex formed was identified by coelution of [14c] moxalactam with the enzyme by using sephadex g-25 gel filtration in the presence of 5.7 m guanidine hydrochloride and by analytical isoelectric focusing. both the side-chain carboxyl group and the 7 alpha-methoxy group of moxalactam were necessary to stabi ... | 1985 | 3874595 |
| the interaction of beta-lactam compounds with chromosomally mediated enzymes: relations to the molecular structure. | the interaction of 25 beta-lactam compounds with both chromosomally mediated beta-lactamases from enterobacter cloacae and citrobacter freundii was studied by enzyme kinetics. all the penams, cephems, and the penem sch 29482 revealed 'competitive inhibition' of both enzymes. the penem required a preincubation period of approximately 5 min before reaching a state of equilibrium between the active and the inactive enzyme. except for the recently developed compound hr 810, newer cephalosporins gene ... | 1985 | 3875455 |
| population analysis of susceptibility to cefotaxime and desacetyl-cefotaxime in staphylococcus and enterobacteriaceae. | population analyses of susceptibility to cefotaxime (ctx) and desacetyl-cefotaxime (dctx) of strains of staphylococcus and some genera of enterobacteriaceae were carried out. dctx, which is the main metabolite of ctx, has antimicrobial activity. the penicillinase-producing strains of s. aureus and s. epidermidis were homogeneous as regards susceptibility to both agents. ctx was about 4-8 times more active than dctx. the methicillin-resistant strains contained a sub-population of resistant bacter ... | 1985 | 3875967 |
| aztreonam in the treatment of serious orthopedic infections. | aztreozam was evaluated in the treatment of a variety of orthopedic infections. included were 17 patients with osteomyelitis, three with purulent arthropathy with prostheses, and 16 with superficial infections secondary to trauma or surgical procedure. pathogens were gram-negative bacilli sensitive to aztreonam. concomitant antibiotics were administered for gram-positive cocci that were present initially or by superinfection. infecting organisms included pseudomonas aeruginosa (minimal inhibitor ... | 1985 | 3881949 |
| application of in-vitro models: development of resistance. | the development of chromosomal beta-lactam resistance of escherichia coli, enterobacter cloacae and citrobacter freundii was observed by following the bacterial kill-kinetics in an in-vitro model simulating human serum antibiotic concentrations. from sensitive escherichia coli cells mutants arose resistant to aminopenicillins, and to first and second generation cephalosporins, whereas with the ent. cloacae and citro. freundii mutants were also resistant to cefotaxime. resistant mutants from all ... | 1985 | 3884569 |
| pathogenesis of escherichia coli cystitis and pyelonephritis: apparent lack of significance of bacterial motility and chemotaxis towards human urine. | all 45 microscopically motile urinary isolates tested here (37 escherichia coli, two enterobacter cloacae, two citrobacter freundii, three proteus mirabilis and one proteus morganii) were strongly attracted to fresh human urine in a capillary chemotaxis assay. this observation suggested that urine taxis of gram-negative bacteria promotes their invasion of the human lower urinary tract and their ascension to the kidney(s). however, the incidence of motile isolates and their activity in urine taxi ... | 1985 | 3886554 |
| inhibitory activity of cefpiramide on beta-lactamase. | the beta-lactamase stability and inhibitory activity of cefpiramide were investigated. cefpiramide was found to be stable to hydrolysis and inhibited beta-lactamase produced by citrobacter freundii, enterobacter cloacae, morganella morganii, and escherichia coli. kinetic studies showed that cefpiramide is a competitive inhibitor of cephaloridine hydrolysis by e. cloacae beta-lactamase. | 1985 | 3888544 |
| in vitro studies on the antibacterial activities of ym-13115, a new broad-spectrum cephalosporin. | the in vitro antibacterial activities of ym-13115, a new parenteral cephalosporin, were compared with those of ceftazidime, cefoperazone, and cefsulodin. the compound was highly active against the common members of the enterobacteriaceae and 2 to 256 times more active than cefoperazone. ym-13115 was as active as ceftazidime against citrobacter freundii, proteus vulgaris, and morganella morganii and two to four times more active than ceftazidime against escherichia coli, klebsiella pneumoniae, en ... | 1985 | 3890729 |
| aztreonam in the therapy of nosocomial infections in patients with impaired host defenses. | a clinical trial was carried out to evaluate the effectiveness of treatment with aztreonam in hospital-acquired infections. twenty patients (13 men and 7 women) with impaired host defenses and nosocomial infections were treated with aztreonam at a dosage ranging 4 and 6 g/day i.v. at 8 or 12-hour intervals. average length of therapy was 10.35 +/- 4.61 days. the isolated organisms were as follows: escherichia coli (5), pseudomonas aeruginosa (5), klebsiella pneumoniae (3), enterobacter cloacae (1 ... | 1985 | 3891117 |
| rapid and automated detection of salmonella by electrical measurements. | a rapid method for determining the presence of salmonella in food is described. it consists of pre-enrichment in buffered peptone water modified by the addition of dulcitol and trimethylamine oxide, followed by selective enrichment in a selenite-cystine broth with similar modifications. changes in the conductance of the selective enrichment broth are monitored continuously using a suitable impediometric instrument. most of the salmonella spp. tested gave a fast (approximately 100 micros/h) and l ... | 1985 | 3891846 |
| sch 34343 activity against streptococci and beta-lactam-resistant enterobacteriaceae. | the in-vitro activity of sch 34343 was compared with that of cefotaxime, ceftazidime, latamoxef (moxalactam), aztreonam and ampicillin. against pneumococci, sch 34343 was as active as ampicillin, whereas against the other streptococci it was less active than ampicillin but significantly better than the other antibiotics against enterococci. with clinical isolates of enterobacteriaceae resistant to cefotaxime, sch 34343 had mics generally less than 2 mg/l. after introduction of plasmid-mediated b ... | 1985 | 3897168 |
| sch 34343: in-vitro antibacterial activity and susceptibility to beta-lactamases. | the in-vitro activity of the penem sch 34343 was assessed in comparison with third-generation cephalosporins, aztreonam and imipenem. sch 34343 was active, with mics generally 2 mg/1 or less, against a broad spectrum of organisms amongst which were staphylococci (including methicillin-resistant strains), streptococci (though enterococci were less susceptible with sch 34343 mics mostly 4-8 mg/1), anaerobes (including the bacteroides fragilis group), haemophilus influenzae and neisseria gonorrhoea ... | 1985 | 3897169 |
| bacteremias due to citrobacter diversus and citrobacter freundii. incidence, risk factors, and clinical outcome. | from 1974 to 1982, 38 patients developed citrobacter bacteremia at two adult community-teaching hospitals in the detroit medical center (incidence, 1.2 cases per 10,000 discharges). citrobacter accounted for 0.7% of all bacteremias during the study period. of 31 cases reviewed, citrobacter bacteremia frequently developed in elderly patients (65%) and was hospital acquired (77%). initial sites of infection included the urinary tract (39%), gastrointestinal tract (27%), wound (10%), and unknown (1 ... | 1985 | 3899035 |
| [carbenicillin resistance of gram-negative bacteria: incidence, biochemical and genetic determinism]. | of nine hundred ampicillin resistant (amp-r) enterobacteria strains, isolated in hospital between july and december 1981, 73,7% are also carbenicillin-resistant (carb-r). this particular double resistance varies depending upon the species considered: indole positive proteus (23%), enterobacter cloacae (64%), citrobacter freundii (67%), acinetobacter calcoaceticus (73%), proteus mirabilis (75%), serratia marcescens (90%), escherichia coli (91%), providencia stuartii (96%) and klebsiella pneumonia ... | 1985 | 3903620 |
| cefamandole resistance transfer in bacterial strains from two newborn units. | transfer of cefamandole resistance was demonstrated from strains of citrobacter freundii as well as from individual strains of enterobacter cloacae, acinetobacter anitratus and klebsiella pneumoniae isolated from patients in two newborn units. in citrobacter freundii, cefamandole resistance was transferred always with cephalotin resistance as well as with a tem-like beta lactamase (conferring resistance to ampicillin, carbenicillin and azlocillin). citrobacter freundii strains from hospital i we ... | 1985 | 3910723 |
| rapid identification of escherichia coli from routine urine specimens based on macroscopic criteria. | in our laboratory, selected strains of gram-negative rods from urine samples are identified as escherichia coli on the basis of smell and morphology on lactose agar. to investigate the accuracy of this routine practice, 211 consecutive strains were tested in the urea-indole tube of the three-tube method (3-tm), in the pgua test detecting beta-glucuronidase activity and in the simmons' citrate test, to select the strains that were non-e. coli. additional 1022 strains were tested by the indole and ... | 1985 | 3914821 |
| [enzymatic synthesis of 3,4-dihydroxyphenyl-l-alanine by free and immobilized citrobacter freundii cells]. | the citrobacter freundii 62 cells immobilized in paag and possessing the tyrosine-phenol-lyase (tpl) activity catalyse the synthesis of 3,4-dihydroxyphenyl-l-alanine (dopa) from pyrocatechol and ammonium pyruvate. the synthesis of dopa was studied using both free and immobilized bacterial cells. when the concentration of pyrocatechol is over 0.1 m the tpl activity of the cells is inhibited. the concentration of pyrocatechol can be increased up to 0.3 m by using an equimolar mixture of pyrocatech ... | 1985 | 3921951 |
| isolation and characterization of a new endo-beta-galactosidase from diplococcus pneumoniae. | an endo-beta-galactosidase, which hydrolyzes the internal beta-galactosidic linkages of r----glcnac (or galnac) beta 1----3gal beta 1----4glcnac (or glc), was isolated from the culture supernatant of diplococcus pneumoniae. the enzyme, named endo-beta-galactosidase dii, hydrolyzed linear n-acetyllactosamine repeating structures in glycolipids and glycopeptides to release oligosaccharides. the specificity of endo-beta-galactosidase dii is the same as that of escherichia freundii endo-beta-galacto ... | 1985 | 3922410 |
| the in vitro activity and beta-lactamase stability of cefpirome (hr 810), a pyridine cephalosporin agent active against staphylococci, enterobacteriaceae and pseudomonas aeruginosa. | the in vitro activity of cefpirome, a new cyclopyridinium cephalosporin, was evaluated against 947 aerobic and anaerobic bacteria. cefpirome inhibited 90% of escherichia coli, klebsiella spp., citrobacter diversus, morganella morganii, proteus vulgaris, proteus mirabilis, aeromonas spp., salmonella spp., shigella spp. and haemophilus and neisseria species at less than or equal to 0.4 mg/l. it had activity comparable to that of cefotaxime, ceftizoxime, ceftazidime, aztreonam, and moxalactam again ... | 1985 | 3928497 |
| [cefminox concentration in tissues and clinical efficacy of cefminox in acute peritonitis]. | cefminox sodium (cmnx, mt-141), a new semisynthetic cephamycin, having marked resistance to beta-lactamase, and a broad spectrum of antibacterial activity against various bacterial species, including haemophilus influenzae, serratia marcescens and citrobacter freundii, cmnx has higher activity in vivo than in vitro. for therapeutic purpose, cmnx was given in a daily dose of 0.5 g (0.5 g x 1) to 2 g (1 x 2) by intravenous drip infusion for 4 to 8 days to 24 cases with acute peritonitis (17 cases ... | 1985 | 3930784 |
| [distribution and changes in the susceptibility of bacteria isolated from clinical samples. iii]. | this report presents data concerning in vitro activity of antimicrobial agents against citrobacter freundii, enterobacter spp., serratia marcescens and proteus vulgaris isolated from patients with complicated urinary tract infections and against pseudomonas aeruginosa isolated from surgical wounds with postoperative infection and exudate from superficial abscesses. there was a marked increase of resistant strains of c. freundii, enterobacter spp. and s. marcescens to penicillins, ceps or gm. the ... | 1985 | 3930806 |
| [synthesis of tyrosine and 3,4-dihydroxyphenylalanine by citrobacter freundii bacteria from lactic acid]. | a possibility of using lactic acid as a precursor for the synthesis of l-tyrosine and l-3,4-dihydroxyphenylalanine (dopa) by citrobacter freundii 62 and 63 was established. the synthesis of tyrosine from lactic acid occurs at a phenol concentration of less than 0.6%. the conditions were found which enable c. freundii 62 and 63 to synthesize from lactic acid tyrosine and dopa with the yield of 35-38 g/l and 32-33 g/l, respectively. | 1985 | 3931068 |