Publications

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formation of biologically active substances by rhizosphere bacteria and their effect on plant growth. 19704995526
ion-exchange separation and automated assay of complex mixtures of amino acids and hexosamines. 19704985912
[cephalothin and semisynthetic penicillins. in vitro test results on material in a large hospital]. 19674968842
[studies on the mechanism of bacterial endotoxins]. 19664962810
deoxyribonucleic acid homology and taxonomy of agrobacterium, rhizobium, and chromobacterium.hybridization experiments were carried out between high molecular weight, denatured, agar-embedded deoxyribonucleic acid (dna) and homologous, nonembedded, sheared, denatured (14)c-labeled dna from a strain of agrobacterium tumefaciens and rhizobium leguminosarum (the reference strains) in the presence of sheared, nonembedded, nonlabeled dna (competing dna) from the same or different nomen-species of agrobacterium, rhizobium, chromobacterium, and several other organisms. percentage of dna homolo ...19674961629
comparative studies of putrescine degradation by microorganisms. 19664956873
biological properties of streptococcal cell-wall particles. 3. dermonecrotic reaction to cell-wall mucopeptides.abdulla, essa m. (university of north carolina school of medicine, chapel hill), and john h. schwab. biological properties of streptococcal cell-wall particles. iii. dermonecrotic reaction to cell-wall mucopeptides. j. bacteriol. 91:374-383. 1966.-intradermal injection of rabbits and guinea pigs with mucopeptide suspensions produced an acute necrotic lesion which reached maximal severity within 24 hr and gradually subsided with scar formation. necrosis was evident within 4 hr after injection of ...19664955252
properties of an r factor from pseudomonas aeruginosa.an r factor from pseudomonas aeruginosa, which confers resistance to penicillins, kanamycin, and tetracycline, was studied in escherichia coli k-12. the r factor could coexist with f-like or i-like plasmids and therefore constituted a novel compatibility group. the r factor was transferable from e. coli to bacterial genera outside the enterobacteriaceae (pseudomonas and members of the rhizobiaceae) to which transfer of f-like and i-like plasmids could not be demonstrated.19714945193
a study of chromobacterium typhiflavum from the rat. 19694905075
the phosphoglyceride composition of gram-negative bacteria and the changes in composition during growth. 19694898381
the detection of a strain of chromobacterium lividum in the tissues of certain leaf-nodulated plants by the immunofluorescence technique. 19684891921
the nature, intergeneric distribution and biosynthesis of isoprenoid quinones and phenols in gram-negative bacteria.1. twenty-two aerobically grown gram-negative bacteria were analysed for demethylmenaquinones, menaquinones, 2-polyprenylphenols, 6-methoxy-2-polyprenylphenols and ubiquinones. 2. all the eight enterobacteria and both the two facultative organisms (aeromonas punctata and aeromonas hydrophila) examined contain all the compounds listed above. the principal homologues are octaprenyl; in addition lower (down to tri- or tetra-prenyl for the 2-polyprenylphenols) and sometimes higher homologues are als ...19694886765
[bacterial strains agglutinable with serums with anti-shigella a, b, and c in various proportions]. 19684883357
adansonian analysis of the rhizobiaceae. 19684870842
sorption between microorganisms and clay minerals as determined by the electrical sensing zone particle analyser. 19684870493
selective feeding of tubificids on bacteria. 19674864607
mesosome structure in chromobacterium violaceum.exponentially growing cells of the gram-negative bacterium chromobacterium violaceum demonstrate invaginations of the cytoplasmic membrane with a high frequency. these invaginations conform to the ultrastructural appearance of mesosomes of gram-positive bacteria. as many as four mesosomes are observed per cell, each of which may increase the total membrane surface of the cell by 30%. washing of cells in dilute tris(hydroxymethyl)aminomethane buffer effects a distension of the mesosome "neck" and ...19744828310
purification and characterization from chromobacterium violaceum of an enzyme catalyzing the synthesis of gamma-cyano-alpha-aminobutyric acid and thiocyanate. 19734760496
biosynthesis of phenazines. ii. incorporation of (6-14c)-d-shikimic acid into phenazine-1-carboxylic acid and iodinin. 19734733458
detection of "chromobacterium" typhi-flavum in stool specimens. 19724661461
the oxidase activity of chromobacterium. 19734633854
the ortho-nitrophenol (onpg) test and acid from lactose in gram-negative genera.the results are given of the ortho-nitrophenol test for beta-galactosidase production (onpg test) on 588 strains of 123 aerobic species of bacteria, representing 30 genera. apart from some strains of erwinia herbicola (synonym chromobacterium typhiflavum) and of yersinia spp, these strains were not members of the enterobacteriaceae, in which family the onpg test is widely used and well documented. the strains were also tested for acid production from 1, 5, and 10% lactose and the findings are di ...19734587939
genetic and phenetic classification of bacteria. 19734584677
serine dehydratase from bacteria. 19734583868
carbon source utilization tests as an aid to the classification of non-fermenting gram-negative bacteria. 19734571899
successful treatment of systemic chromobacterium violaceum infection. 19744479291
detection of a vibrio sp. by the bacteriophagous nematode pelodera chitwoodi. 19744418570
6 -amidinopenicillanic acids--a new group of antibiotics. 19724402006
bacterial genome sizes determined by dna renaturation studies. 19704396963
"gram-negative" infections of the skin. 19714329494
in-vitro sensitivity of 368 urinary organisms to trimethoprim-sulphamethoxazole and other antibacterials. 19704319442
[comparative study of bacterial phosphomonoesterases and phosphodiesterases hydrolyzing nucleic acids and nucleotides]. 19684310247
biosynthesis of cyanide from [2-14c-15n]glycine in chromobacterium violaceum. 19694309602
the tryptophan hydroxylase of chromobacterium violaceum. 19744279915
[c02 formation by microorganisms of different taxonomic groups cultivated on media with carbohydrates and hydrocarbons]. 19724272102
[comparative assessment of thermophilic hydrocarbon-oxidizing microorganisms in relation to their protein and amino acid content]. 19724263937
[naphthalene oxidizing bacteria in ground waters of oil beds]. 19654221916
carriage of potentially pathogenic bacteria in the hair. 19744212981
[dissolution of gold by microorganisms and the products of their metabolism]. 19744210177
[newly discovered sugars in the specific polysaccharides of bacteria]. 19724208820
studies on the lipase of chromobacterium viscosum. 3. purification of a low molecular weight lipase and its enzymatic properties. 19744208235
[the effect of ultraviolet rays on the microflora of meat]. 19734207537
hemingway's sweating waiter. 19684173715
studies on the role of microorganisms in the life of cultivated plants. 3. origin of the bacterial substances stimulating the growth of plants. 19664160456
[defective phages in chromobacterium]. 19724146499
the intracellular organization of bacteriophage tail-like particles in cells of chromobacterium violaceum following mitomycin c treatment. 19734122865
organization of bacteriophage tail-like particles in cells of chromobacterium violaceum.a strain of chromobacterium violaceum has been isolated which produces bacteriophage tail-like particles in high numbers. the extracellular morphology and the intracellular arrangement of these particles are described.19724112258
chromobacterium violaceum adenitis acquired in the northern united states as a complication of chronic granulomatous disease. 19854080593
quantitative studies of heat-stable proteinase from pseudomonas fluorescens p1 by the enzyme-linked immunosorbent assay.pseudomonas fluorescens p1 is a psychrotrophic bacterium isolated from milk. proteinase p1, the main extracellular heat-stable proteinase fraction of p. fluorescens p1, has been purified to homogeneity. a procedure with a sandwich enzyme-linked immunosorbent assay, using microplates and alkaline phosphatase conjugate was shown to detect 0.25 ng of proteinase p1 in 1 ml of reconstituted skim milk or defatted cream. the method offers the combination of sensitivity and specificity for the detection ...19853920965
novel potentiators of beta-lactam antibiotics. isolation of sq28,504 and sq28,546 from chromobacterium violaceum.two novel compounds, sq28,504 and sq28,546 are produced by chromobacterium violaceum. these compounds enhance the antibacterial activity of beta-lactam antibiotics against gram-negative organisms. both sq28,504 and sq28,546 induce morphological changes in the presence of beta-lactam antibiotics. only sq28,546 has weak antimicrobial activity against several gram-negative organisms.19853839229
[fatal septicemia due to chromobacterium violaceum].a 2-year old boy was suffered from an infection caused by chromobacterium violaceum. this is the first case of c. violaceum infection reported in taiwan. the boy was treated with chloramphenicol and gentamicin but died after one month of illness due to the incooperation of patient's family. the causative agent was isolated from patient's blood and scar. complete bacteriologic characteristics and antimicrobial susceptibility pattern of this isolate are reported.19863816371
isolation and characterization of aerobic heterotrophic bacteria from natural spring waters in the lanjaron area (spain).aerobic, heterotrophic bacteria were isolated from nine natural mineral water springs in the lanjaron area of spain over the period july 1980 to may 1981. the mineral waters contained few bacteria (mean counts 26-5275 cfu per 100 ml) and the bacterial flora of all nine springs was very similar. most of the isolates were gram-negative rods (90%), and among these pseudomonas spp. and members of the flavobacterium-cytophaga-flexibacter group were numerically dominant. aeromonas-vibrio and enterobac ...19863781942
nonfatal chromobacterial sepsis.we have described a case of multiple liver abscesses caused by chromobacterium violaceum. this organism should be considered in any case of unexplained liver abscess occurring in the southeastern united states. successful treatment of liver abscesses caused by this organism may be accomplished with appropriate antibiotic therapy.19863750002
first case report from argentina of fatal septicemia caused by chromobacterium violaceum.a case of chromobacterium violaceum fatal septicemia is reported. the microorganism was isolated from six blood cultures and two suppurated skin lesions. it is the first such case described in argentina, and it reinforces the need for prolonged treatment and careful clinical evaluation to ensure complete remission of human infections caused by this bacterium.19863711283
cell-free biosynthesis of arphamenine a.arphamenine a was synthesized in a cell-free system obtained from the arphamenine-producing strain, chromobacterium violaceum bmg361-cf4. l-[14c]-phenylalanine was converted to beta-phenylpyruvic acid by phenylalanine amino-transferase obtained from the 10,000 x g supernatant (s10 fraction). [14c]-benzylmalic acid was synthesized from beta-phenylpyruvic acid with [14c]-acetyl-coa in the s10 fraction. [14c]-benzylsuccinic acid was formed from beta-phenylpyruvic acid with [14c]-acetyl-coa and atp ...19863707594
glucose-6-phosphate dehydrogenase deficiency, neutrophil dysfunction and chromobacterium violaceum sepsis. 19873681551
bacteremia caused by cdc group ve-1 in previously healthy patient with granulomatous hepatitis.this is a case report of cdc group ve-1 bacteremia in an otherwise healthy patient with granulomatous hepatitis.19873667927
[mobilization of granulocyte-macrophage colony-forming cells (cfu-gm) into the peripheral blood of the mouse by ch. violaceum endotoxin]. 19873603064
chromobacterium violaceum phenylalanine 4-monooxygenase. 19873600378
mercury-resistance and mercuric reductase activity in chromobacterium, erwinia, and bacillus species. 19873580613
microbial cyanide metabolism. 19863532718
unusual mucocutaneous infections in immunosuppressed patients with leukemia--expansion of an earlier study. 19863513146
chromobacterium violaceum meningitis--a case report. 19873449453
[use of chromobacterin to control the larvae of blood-sucking mosquitoes]. 19873448483
aerocyanidin, a new antibiotic produced by chromobacterium violaceum.aerocyanidin, a new antibiotic containing an isonitrile group, has been isolated from fermentations of chromobacterium violaceum atcc 53434. structure 1 was assigned on the bais of spectroscopic characterization of the antibiotic and of a degradation product that results from treatment with base. the antibiotic is primarily active against gram-positive bacteria.19883372351
aerocavin, a new antibiotic produced by chromobacterium violaceum.a new antibiotic, aerocavin, has been isolated from fermentation broths of a non-pigmented strain of chromobacterium violaceum. the structure 1 was deduced from its spectroscopic properties and x-ray diffraction analysis. aerocavin exhibits activity in vitro against gram-positive and gram-negative bacteria.19883372350
[role of halophilic and halotrophic bacteria in the chronic problems of long-term dialysis]. 19873319061
natural microbiologic hazards of the aquatic environment. 19873311341
[chromobacterium violaceum infection. presentation of a case with fatal outcome]. 19863299554
occurrence, transfer and mobilization in epilithic strains of acinetobacter of mercury-resistance plasmids capable of transformation.a 7.8 kb plasmid (pqm17) encoding mercury resistance was isolated from two epilithic strains of acinetobacter calcoaceticus. the plasmid had a broad host range when mobilized by rp1, transferring into pseudomonas aeruginosa, p. putida, p. fluorescens, escherichia coli, proteus vulgaris and chromobacterium sp. with frequencies ranging from 5.3 x 10(-9) to 4.6 x 10(-4) per recipient. the plasmid could be transferred into a. calcoaceticus bd413 using intact cells of donor and recipient bacteria (i. ...19883254940
[the role of gram-negative non-fermenting bacteria in hospital infections]. 19883237527
comparison of the in vitro activity of ciprofloxacin and 24 other antimicrobial agents against clinical strains of chromobacterium violaceum.eleven clinical strains of chromobacterium violaceum were tested for their susceptibility to 25 antimicrobial agents. ciprofloxacin was the most active of the compounds tested although norfloxacin and pefloxacin were highly active. no resistance was detected to mezlocillin, piperacillin, apalcillin, imipenem, and aztreonam while a single strain was resistant to ticarcillin. among the cephalosporin/cephamycin group only cefotetan showed good in vitro activity. gentamicin was more active than amik ...19883168426
structural heterogeneity regarding local shwartzman activity of lipid a.the relation of chemical structure to local shwartzman activity of lipid a preparations purified by thin-layer chromatography from five bacterial strains was examined. two lipid a fractions from e. coli f515--ec-a2 and ec-a3--exhibited strong activity, similar to that of previous synthetic e. coli-type lipid a (compound 506 or la-15-pp). the ec-a3 fraction contained a component that appeared to be structurally identical to compound 506, and the main component of ec-a2 fraction was structurally s ...19883057331
phenylalanine hydroxylase from chromobacterium violaceum is a copper-containing monooxygenase. kinetics of the reductive activation of the enzyme.pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum contains a stoichiometric amount of copper (cu2+, 1 mol/mol of enzyme). electron paramagnetic resonance spectroscopy of the enzyme indicates that it is a type ii copper-containing protein. the oxidized enzyme must be reduced by a single electron to be catalytically active. dithiothreitol was found to be an effective reducing agent for the enzyme. electron paramagnetic resonance data and kinetic results indicate the formati ...19863024714
the mechanism of action of phenylalanine hydroxylase. 19863014980
phenylalanine 4-monooxygenase from chromobacterium violaceum. 19872885717
partial purification and characterization of adp sulfurylase from the purple sulfur bacterium thiocapsa roseopersicina.high activities of adp and atp sulfurylase were found in the soluble protein fraction of thiocapsa roseopersicina strain 6311 (dsm 219). adp sulfurylase was partially purified and characterized. it was a very labile soluble enzyme with a molecular weight of 250,000. the optimum ph was 7.5 and the optimal temperature 35 degrees c. under test conditions the apparent km values were determined to be 0.33 mm for adenylylsulfate and 13 mm for phosphate.19882855979
a comparative study of virulent and avirulent strains of chromobacterium violaceum.a clinical isolate and a soil isolate of chromobacterium violaceum were compared to determine differences in virulence-related characteristics. purified lipopolysaccharide (endotoxin) from the virulent, clinical strain was more reactive than that from the avirulent soil strain as determined by the limulus amebocyte lysate assay. there were no differences in hemolysin or cyanide production between the two strains. the virulent strain was more resistant to phagocytosis and intracellular killing by ...19882843271
adduct formation between the cupric site of phenylalanine hydroxylase from chromobacterium violaceum and 6,7-dimethyltetrahydropterin.the interaction of pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum with the cofactor analogue 5-deaza-6-methyltetrahydropterin and the cofactor 6,7-dimethyltetrahydropterin (dmph4) has been investigated by multifrequency electron spin resonance (esr) spectroscopy. 5-deaza-6-methyltetrahydropterin, which lacks the n-5 nitrogen present in the pyrazine ring of dmph4, binds tightly to the cupric form of the enzyme; however, no changes are observed in the esr parameters of t ...19872822093
occurrence of drug-resistant bacteria in communal well water around port harcourt, nigeria.a total of 108 raw water samples was collected from 36 wells at nine shanty settlements around port harcourt, nigeria, over a period of 7 months. samples were analysed for their bacteriological quality. selected bacterial strains isolated from the samples were tested for their susceptibility to ten commonly used antibiotics. the organisms isolated include pseudomonas spp., klebsiella spp., staphylococcus spp., proteus spp., enterococcus faecalis, aeromonas spp., escherichia coli, chromobacterium ...19892776852
an acute pleuropneumonia in a pig caused by chromobacterium violaceum.a 2.5-month-old, 30 kg duroc pig died 10 days after showing clinical signs of dyspnoea and diarrhoea. acute necrotizing and fibrinous pleuropneumonia with locally extensive lesions was found. chromobacterium violaceum was isolated from pneumonic lung tissues and intratracheal inoculation of a pure culture into two spf pigs reproduced lesions similar to those found in the natural infection.19892760278
near-fatal septicaemia with chromobacterium violaceum.chromobacterium violaceum is a gram-negative organism which normally inhabits water and soil. human infection is unusual and is associated with a high mortality rate. we describe a typical case of disseminated infection with chr. violaceum in a male carpet cleaner. the possible origin and treatment of the infection is discussed.19892733620
mechanistic studies on phenylalanine hydroxylase from chromobacterium violaceum. evidence for the formation of an enzyme-oxygen complex.steady-state kinetic analysis of pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum indicated that the enzyme follows a partially ordered reaction mechanism. the data suggested that oxygen is the first substrate to bind to the enzyme. this result was further supported by rapid-quench experiments in which the enzyme-oxygen complex was trapped to yield product. additional support for the presence of an enzyme-oxygen complex was derived from magnetic susceptibility measuremen ...19892719947
the pattern of protein synthesis induced by heat-shock of the moderately halophilic bacterium chromobacterium marismortui: protective effect of high salt concentration against the thermal shock.the protein synthetic response to heat shock of the moderately halophilic bacterium chromobacterium marismortui was examined. upon exposure to elevated temperature there is an increased synthesis of a specific subset of proteins (heat shock proteins-hsps) in the molecular weight region of 15 to 90 kd, while normal protein synthesis is severely repressed. the synthesis of hsps reaches a maximum 5 min after heat shock at 42 degrees c. cells recovered their normal protein synthesis patterns rapidly ...19892716536
effect of growth conditions on production of violacein by chromobacterium violaceum (bb-78 strain).1. chromobacterium violaceum (strain bb-78 isolated in brazil) produces violacein, a substance potentially useful in phototherapy and with antibiotic and trypanocide activity. culture conditions were optimized for the production of violacein and changes in nutrients, temperature and ph were correlated to cellular growth. 2. methionine was the only absolute requirement for growth. alanine, arginine, tryptophan and vitamin b12 stimulated growth and the microorganism utilized both the d and l forms ...19892620169
bacterial colonization of domestic reverse-osmosis water filtration units.we have analyzed the bacterial content of water from the reservoirs of 300 reverse-osmosis units installed in households. the heterotrophic plate counts on r2a medium (20 and 35 degrees c) ranged from 0 to 10(7) colony forming units per millilitre (cfu/ml). most reservoirs contained water with bacterial counts between 10(4) and 10(5) cfu/ml. the bacteria identified were pseudomonas (not aeruginosa), alcaligenes or moraxella, acinetobacter, flavobacterium, and chromobacterium. this report emphasi ...19892611732
temperature-dependent incorporation of 4-amino-l-arabinose in lipid a of distinct gram-negative bacteria.the presence and the relative amount of 4-amino-l-arabinose in lipopolysaccharides of members of the enterobacteriaceae family and in a single strain of chromobacterium violaceum has been studied with regard to growth-temperature dependent variations. changes in the presence and the amount of 4-amino-l-arabinose (4-aa) were observed in almost all cases, but the variations observed were not consistent among different species. while salmonella minnesota and proteus mirabilis showed higher levels o ...19892583477
bacterial ethylene synthesis from 2-oxo-4-thiobutyric acid and from methionine.the ability of selected bacterial cultures to synthesize ethylene during growth in nutrient broth supplemented with methionine or 2-oxo-4-methylthiobutyric acid (kmba) was examined. although most cultures transformed kmba into ethylene, only those of escherichia coli spao and chromobacterium violaceum were able to convert exogenously added methionine to ethylene. in chemically defined media, e. coli spao produced the highest amounts of ethylene from methionine and kmba. this capability was affec ...19892559143
bacterial chemistry-iii: preliminary studies on trypanosomal activities of chromobacterium violaceum products.it has been found that the pigment-i from chromobacterium violaceum, 3-[1,2-dihydro5-(5-hydroxy-1h-indol-3-yl)-2-oxo-3h-pyrrol-3-yli diene] 1,3-dihydro-2h-indol-2-one, has trypanocide activity. the formylated derivatives, pigment-iii, immobilized 100% of the trypanosoma cruzi at a level of 46 microm after 48 h of interaction with a total growth inhibition in the same period. pigment-i exhibited low toxicity and a dna synthesis inhibition similar to that of nifurtimox, a known trypanocide compoun ...19892512826
pneumonia and meningitis caused by a new nonfermentative unknown gram-negative bacterium.seven isolates of an unclassified bacterium resembling flavobacterium spp. were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, protein profile analysis, and serologic data. the unclassified isolates were differentiated from flavobacterium meningosepticum, flavobacterium odoratum, flavobacterium balustinum, flavobacterium strain iib, chromobacterium violaceum, aquaspirillum serpens, and pseudomonas spp. the bacterium ...19892504766
the use of tween 20 in a sensitive turbidimetric assay of lipolytic enzymes.a turbidimetric esterase assay was developed using a tween 20 solution in the presence of cacl2 and lysobacter enzymogenes esterase (ec 3.1.1.1) as the enzyme source. the reaction was followed by measuring the increase in the optical density at 500 nm (od500) due to the hydrolytic release of the fatty acids from tween 20 and their precipitation as the calcium salts. concentrations of 1.8% tween and 3 mm cacl2 were found to be optimal for the assay of 0.036 to 0.15 esterase units in a 4-ml reacti ...19892501015
comparative genetic organization of incompatibility group p degradative plasmids.plasmids that encode genes for the degradation of recalcitrant compounds are often examined only for characteristics of the degradative pathways and ignore regions that are necessary for plasmid replication, incompatibility, and conjugation. if these characteristics were known, then the mobility of the catabolic genes between species could be predicted and different catabolic pathways might be combined to alter substrate range. two catabolic plasmids, pss50 and pss60, isolated from chlorobipheny ...19902254257
endotoxin size in hemodialysis solutions: modifications in presence of concentrated salt solutions and bacterial products. 19902183572
hydrodynamic characterization of chromobacter viscosum lipase. 19902083651
solution behaviour of chromobacter viscosum and pseudomonas sp. lipases. no evidence of self-association.1. the size of two bacterial lipases was studied by sds/page, sedimentation velocity and sedimentation equilibrium to test for possible self-association behaviour. 2. mr values of selected lipases were obtained from sds/page and sedimentation-velocity measurements, together with an absolute determination by sedimentation equilibrium 3. the mr values obtained in a variety of aqueous solvents indicate that lipases do not self-associate in solution, suggesting the absence of surface hydrophobic pat ...19911996958
beta-d-glucuronidase (bdg) activity of gram-negative bacteria.bdg is an inducible enzyme that is encoded by the uida gene in escherichia coli. genetic sequences of this gene are present in most if not all e. coli strains regardless of the bdg phenotype. expression of bdg activity can be influenced by lactose-induced catabolite repression or genetic mutations. salmonella, shigella and yersinia strains frequently exhibit positive bdg reaction. bdg activity of strains belonging to genus edwardsiella, serratia, yersinia, vibrio, erwinia, alcaligenes, acinetoba ...19911817425
survival of the fish pathogen aeromonas salmonicida in seawater.survival of aeromonas salmonicida in natural (non-sterile) seawater, as determined from colony counts on marine agar, was found to be influenced by the presence of potentially inhibitory organisms, i.e., acinetobacter, aeromonas hydrophila, chromobacterium, escherichia coli, flavobacterium and pseudomonas, and their metabolites. yet, samples, thought to be devoid of culturable a. salmonicida, were found to contain cells, which were filterable through 0.22 and 0.45 microns millipore millex porosi ...19911769545
[first isolation of chromobacterium violaceum strains, also achromogenic, in drinking water]. 19901715722
reverse enzyme synthesis in microemulsion-based organo-gels.lipase from three different sources has been immobilised in microemulsion-based gels (mbgs) with retention of catalytic activity. such lipase-containing mbgs prove to be novel solid-phase catalysts for use in apolar organic solvents such as n-heptane. using these systems, preparative-scale synthesis of a wide variety of esters under mild conditions was possible with products easily isolated and obtained in high yield. stereoselective esterification of octan-2-ol was observed for all three lipase ...19911707672
cloning and expression of chromobacterium violaceum phenylalanine hydroxylase in escherichia coli and comparison of amino acid sequence with mammalian aromatic amino acid hydroxylases.the complete amino acid sequence (296 amino acids) of chromobacterium violaceum phenylalanine hydroxylase (pah) was determined by nucleotide analysis of a dna clone isolated using both a synthetic oligonucleotide probe based on the nh2-terminal amino acid sequence and an antibody against this enzyme. the apal i fragment (approximately 1.9 kilobase pairs) containing the entire pah gene was subcloned in pbluescript ii and induced by isopropyl-beta-d-thiogalactopyranoside. in order to eliminate fus ...19911655752
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