PMID(sorted ascending)
studies on the mechanism of lipase reaction. iii adsorption of chromobacterium lipase of hydrophobic glass beads. 19765197
purification and properties of the extracellular metallo-proteinases of chromobacterium lividum (ncib 10926).four extracellular proteolytic enzymes (i-iv) (ec 3.4.22.-) were identified in static cultures of chromobacterium lividum (ncib 10926) by agar gel electrophoresis and isoelectric focusing. proteinases i-iii were freed of non-enzymic protein by chromatography on teae-cellulose and cm-cellulose. the enzyme mixture was then fractionated in a ph gradient by isoelectric focusing. all three enzymes were shown to be heat-labile metallo-enzymes. optimal activity occurred at ph 5.6 for enzyme i and at ph ...19769995
study of the nature of the associated microflora in the prolonged substrateless cultivation of beet plants.the purpose of the present investigation was to study variations in the population density of microorganisms, their qualitative and quantitative composition during substrate-free cultivation of beats. the results obtained show that the microbial number in the nutrient solution increased by the time of intensive growth of beats and decreased by the time of harvesting. the number of root microorganisms was much greater than the total number of microorganisms in the nutrient solution. oligonitrophi ...197717774
purification of lipase from chromobacterium viscosum by chromatography on palmitoyl cellulose.palmitoyl cellulose was used to adsorb the extracellular lipase triacylglycerol acyl-hydrolase ec of chromobacterium viscosum from crude enzyme solution, and the adsorbed enzyme was eluted with a suitable detergent, such as adekatol 45-s-8 or triton x-100. the enzyme was then purified by chromatography on a palmitoylated gauze column with an overall recovery of 71% and an increase in the specific activity of 11-fold from the supernatant fluid of bacterial cultures. further purification p ...197719442
studies on the production of extracellular proteinases by a non-pigmented strain of chromobacterium lividum isolated from abattoir effluent. 197829882
phenylalanine hydroxylase from chromobacterium violaceum. purification and characterization.phenylalanine hydroxylase was purified approximately 3000-fold to apparent homogeneity with a 13% yield and crystalized from l-phenylalanine-induced cells of chromobacterium violaceum. the enzyme was shown to be composed of a single polypeptide chain with an estimated molecular weight of approximately 32,000. some of the physical properties of the enzyme include: a stokes radius of 26.0 a, a sedimentation coefficient of 2.71 s, a diffusion coefficient of 8.20 x 10(-7) cm2/s, a frictional ratio o ...197933985
immunological relationships of bacterial l-asparaginases.rabbit antisera against l-asparaginase preparations from escherichia coli, erwinia carotovora, citrobacter sp. and chromobacterium violaceum showed on immunoelectrophoresis that only the enzymes from e. coli and citrobacter are immunologically related. purified preparations had to be used to determine the immunological cross-reactions. immunoelectrophoresis at different ph values yielded the zero mobility points of the enzymes. the activity of the er. carotovora preparation was enhanced up to fo ...197654404
antibodies to bacterial l-asparaginases.complement fixation has been shown to occur when asparaginase reacts with specific antibody. this is as a result of an antigenic determinant common to the four bacterial asparaginases. patients on asparaginase therapy may produce antibodies and these could be demonstrated by complement fixation.197775527
the respiratory system of chromobacterium violaceum grown under conditions of high and low cyanide evolution.the particulate fraction of disrupted chromobacterium violaceum grown under cyanide-evolving conditions was unable to oxidize ascorbate plus n,n,n',n'-tetra-methyl-p-phenylenediamine (tmpd), but oxidized nadh and succinate by a linear respiratory pathway which was very resistant to inhibition by cyanide. when the bacteria were grown under conditions where little cyanide evolution occurred, particulate fractions developed the ability to oxidize ascorbate-tmpd by a pathway highly sensitive to cyan ...1975172598
hydroxylation of tryptophan and its control in brain. 1975241047
adsorption and selection of rhizobia with ion-exchange papers.ion exchange papers were used to study the adsorption of 32p-labelled rhizobia on defined surfaces. two strains of rhizobium japonicum and one each of r. leguminosarum and r. lupini were compared with escherichia coli and bacillus subtilis. the ratio of adsorption to strong and to weak acid papers/strong and weak basic papers was consistantly higher for all rhizobial strains compared to the other bacteria. the process of desorption by increasing the ion-concentration causes about 35% desorption ...1975242293
[biochemical tests and nutritional requirements of chromobacterium violaceum]. 1977279056
chromobacterium violaceum infections. report of a fatal case.a case of systemic chromobacterium violaceum infection, believed to be the first reported in a papua new guinean, is described. autopsy disclosed multiple liver abscesses and the diagnosis was made by recovery of the organism from one of the abscesses. aspects of management of this uncommon infection are discussed.1979299336
[infections due to chromobacterium, serratia, erwinia and flavobacterium (the so-called chromobacterioses)]. 1978361381
microbiology problem: chromobacterium violaceum infection. 1979386795
[combination of 5-fluorocytosine and amphotericin b in the treatment of chromomycosis].the authors discuss the problem of resistance of chromomycosis to 5-fluorocytosine (5-fc), observed in a previous study in 7 patients initially treated with the drug and subsequently treated with 5-fc associated with other medicaments. all the associations, including that with amphotericin b, failed to overcome resistance. experiments in mice and "in vitro" show a synergism between 5-fc and amphotericin b against "c. albicans", "c. neoformans" and "fonsecaeae pedrosoi" already confirmed in huma ...1979398931
fatal infection by chromobacterium violaceum--clinical and bacteriological study. 1979457214
osteomyelitis associated with chromobacterium violaceum sepsis. a case report. 1979479248
stimulation of chromobacterium lipase activity and prevention of its adsorption to palmitoyl cellulose by hydrophobic binding of fatty acids.fatty acids prevented adsorption of purified chromobacterium lipase [triacylglycerol acylhydrolase, ec] onto palmitoyl cellulose (pal-c) and also increased the activity of the purified lipase. these effects increased with increase in the concentration and chainlength (up to 16 carbon atoms) of the fatty acids, and long-chain unsaturated fatty acids, such as oleic acid, linoleic acid and erucic acid, were most effective. when the lipase was adsorbed (immobilized) on pal-c, its activity wa ...1978659403
less frequently reported gram-negative fermentative rods.the clinical significance and distinguishing characteristics of less frequently reported gram-negative fermentative rods (pasteurella, yersinia, vibrio, aeromonas and plesiomonas, enterobacter agglomerans, and chromobacterium) are described. increased awareness of these organisms, a knowledge of possible sites in which they cause infection, and identification criteria which will separate them from similar bacteria will prepare the clinical microbiologist for the isolation and identification of t ...1976769548
one-tube oxidation-fermentation methods: limitations posed by atypical fermentative reactions.atypical fermentation in the two-tube method of hugh and leifson (1953), whereby acid was produced in the sealed tube but not in the open tube, was determined to be the result of a preferential metabolism of peptone over the reactant carbohydrate. these atypical fermentations are only partially detected by one-tube oxidation-fermentation methods, and as such they constitute a source of limitation to the routine use of these abbreviated techniques for oxidative-fermentation determinations.1976776087
[formation of l-asparagine and l-glutamine deamidases by bacterial cultures].among studied 40 bacterial cultures, 17 strains catalysed hydroxylaminolysis of i.-asparagine and l-glutamine, and among these cultures seven strains belonged to the pseudomonas genus. extracts of the cells of ps. boreopolis 526 (mgu), ps. aurantiaca ibfm b-14, and ps. septica ibfm b-40 had the maximum deamidase activity during the stage of decelerated growth. the activity of l-asparaginase 2 was practically the same upon various ways of disintegration of the cells of ps. aurantiaca ibfm b-14: b ...1976781474
discrimination of rhizobium japonicum, rhizobium lupini, rhizobium trifolii, rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate.rhizobium strains (one each of rh. japonicum, rh. lupini, rh. leguminosarum) take up 2-ketoglutaric acid in general much faster and from lower concentrations in the medium than strains of escherichia coli, bacillus subtilis and chromobacterium violaceum. a strain of enterobacter aerogenes, however, is more similar to some rhizobium strains. the same strains of rhizobium take up also phosphate much faster and from lower concentrations than the other bacteria tested. 4 strains of rh. lupini proved ...1976818969
chromobacterium violaceum infection in a nonhuman primate (macaca assamensis).chromobacteriosis caused by chromobacterium violaceum was diagnosed as an assam macaque, macaca assamensis, that died 4 days after receipt of the yerkes primate center, it was received from a primate facility in florida where it has been housed with a group of rhesus monkeys for 5 years. the animal died suddenly without showing any signs of clinical illness. necropsy findings included extensive hepatic necrosis with the formation of multiple large cavitary lesions. foci of necrosis were also fou ...1976824500
the chemical structure of the lipid a component of lipopolysaccharides from chromobacterium violaceum nctc 9694.the chemical structure of the lipid a component of lipopolysaccharides from chromobacterium violaceum nctc9694 was studied. sequential treatment of lipopolysaccharide with alkali, acid, sodium borohydride and hydrazine allowed the isolation of a reduced glucosamine disaccharide. according to methylation studies and enzymic analysis with beta-n-acetylglucosaminidase the d-glucosamine residues are beta(1 leads to 6) linked. the disaccharide carries two phosphate groups, one being linked glycosidic ...1977862618
pathogenicity of nonpigmented cultures of chromobacterium violaceum.nonpigmented cultures of chromobacterium violaceum have been found to be similar to pigmented cultures in their virulence for mice and the pathology of their infections. clinicians and microbiologists should be prepared to consider nonpigmented c. violaceum in their differential diagnoses of infections caused by gram-negative bacteria. the laboratorian who is not aware of this possibility is likely to erroneously identify nonpigmented strains of c. violaceum as members of closely associated gene ...1977874071
group ve-2 (chromobacterium typhiflavum) ve-2 (chromobacterium typhiflavum) bacteremia occurred in a severely traumatized, post-neurosurgical patient. a discussion of the clinical occurrence of the organism, bacteriological characteristics, and antibiotic sensitivities is presented.1977914996
studies on the lipase of chromobacterium viscosum. v. physical and chemical properties of the lipases. 1977922980
antibacterial activity of marine violet-pigmented alteromonas with special reference to the production of brominated compounds.the synthesis of several types of antibiotics was investigated in four strains of violet-pigmented bacteria belonging to the species alteromonas luteo-violaceus. two of the strains simultaneously produce an antibiotic polyanionic polysaccharide, weakly bound to the cells and diffusing throughout the medium, and two intracellular brominated bactericidal substances. the third strain only synthesizes the polyanionic antibiotic. the fourth one is totally inactive. the macromolecular antibiotic, prob ...1976974909
studies on the mechanism of lipase reaction. iv. action of the lipase from chromobacterium on monomeric p-titrophenyl acetate. 1976975437
beta-lactamase activity in chromobacterium violaceum.a strain of chromobacterium violaceum isolated from a fatally infected patient was found to produce a beta-lactamase. when the organism was grown in drug-free medium, beta-lactamase activity was barely detectable, but when it was grown in the presence of penicillin g, a much larger amount of activity was produced. the beta lactamase was active primarily against cephalosporins; it was sensitive to inhibition by cloxacillin but resistant to p-chloromercuribenzoate. thus this enzyme closely resembl ...1976977998
effective method for activity assay of lipase from chromobacterium viscosum.a method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, ec] excreted from chromobacterium viscosum into the culture medium; olive oil emulsified with the aid of adekatol 45-s-8 (a non-ionic detergent, the ethoxylate of linear sec-alcohols having chain lengths of 10--16 carbon atoms) was used as the substrate. this method was specifically effective for chromobacterium lipase acitvity assay, and was approximately twice as sensitive as the conventional method, ...19761002673
[microbiological studies of the wanda lake (antarctica)].the deep-water (68 m) wanda lake in the antarctica is noticeable by three characteristics: a relatively high temperature in the bottom layer, elevated salinity, and the presence of h2s. only several (less often, dozens) saprophytic microorganisms per 40 ml of water are encountered in the lake. the total number of bacteria varies from 6-10(3) to 172-10(3) cells/ml. the highest content of the total bacterial population, and saprophytes, is found in intermediate layers, 30 and 40-50 m deep, respect ...19761012048
improvements of the membrane filter method for dna:rrna hybridization.we describe and recommend the following improvements of dna:rrna membrane filter hybridization methods. one of our aims was to avoid dna release from filter discs during hybridization. 1. our hybridization conditions are 2 ssc in aq. dest., with 20% formamide, 50 c, overnight for 16 hr. 2. duplexing is over in 8-10 hr. 3. formamide has to be very pure (o.d. less than or equal to 0.2/cm light path at 270 nm). 4. rnaase treatment: 250 mug/5 ml 2 ssc/filter at 37 c for 1 hr. 5. our conditions for s ...19751082296
a mixed bacterial population in a continuous culture with and without kaolinite.chromobacterium lividum and a pseudomonas sp. were grown in pure and mixed continuous culture with and without the clay-mineral, kaolinite. irrespective of the growth conditions, c. lividum adhered to the wall of the culture vessel whereas the pseudomonas sp. showed no such tendency, at least visually. during mixed culture studies, the organism which was initially established in the culture dominated. the ratio between c. lividum and the pseudomonas sp. was about 20:1 when c. lividum was first e ...19751089463
effect of chlorpromazine on the respiration and hexose monophosphate dehydrogenases of gram-negative bacteria.the drug chlorpromazine inhibits respiration in several gram-negative bacteria. the enzymes glucose-6-phosphogluconate dehydrogenase and 6-phosphogluconate dehydrogenase from these microorganisms are not affected by the drug.19751090352
a maximal predictive classification of klebsielleae and of the yeasts.the concepts of the numerical method of maximal predictive classification are illustrated with classifications of 13 species of enterobacteria and of 434 species of yeast. the method seeks to classify into a specified number of classes (k) such that more correct statements can be made about the constituent members than with any other classification. the best choice of k relates to the separation of the classes as measured by the average number of correct statements made for an individual assigne ...19751113078
selective media for the enumeration of chromobacterium spp. in soil and water. 19751116981
metabolism of n-carbobenzoxyl-l-tryptophan by chromobacterium violaceum.chromobacterium violaceum (atcc 12472) metabolizes n-carbobenzoxyl-l-tryptophan into its 2', 3'-dehydro-derivative, and indole-3-propionic acid into indole-3-acrylic acid. the biotransformation accurs in resting cell incubations, and in growing cultures of the bacterium. tryptophan in fermentation media enhances the ability of chromobacterium violaceum to perform the conversion by an undetermined mechanism. the amino acid also prevents stored cultures from losing the ability to accomplish the ...19751125258
taxonomic position and seasonal variations in marine neritic environment of some gram-negative antibiotic-producing bacteria.six marine bacteria which synthesize macromolecular antibiotics were isolated from neritic waters on the french mediterranean coast, and their frequency recorded over two successive years. they appeared in relatively large numbers during the period august to december, and can be identified as marine pseudomonads; however, the low guaninecytosine ratio of their dna, lack of catalase and specific self-inhibition are not compatible with the characteristics of the genus pseudomonas. two produced vi ...19751141855
letter: carbohydrate fermentation, kia, and tsi agar reactions of chromobacterium violaceum. 19751163494
isolation and identification of a genus, chromobacterium, not previously found on processed organism identified as chromobacterium lividum was isolated from processed poultry in georgia.19751171656
studies on the mechanism of the lipase reaction. ii. comparative studies on the adsorption of lipases and various proteins at the air-water interface.adsorption of lipases (ec and various proteins at the air-water interface has been investigated in relation to the mechanism of lipase reaction. aqueous solutions of lipases and denaturated proteins show surface activity as strong as that of synthetic detergents. however, ths surface activity of esterases and various other proteins is little or none. by foam fractionation it was shown that lipases were adsorbed at the air-water interface and the adsorption followed the equation of langm ...19751171698
characteristics of yellow-pigmented nonfermentative bacilli (groups ve-1 and ve-2) encountered in clinical bacteriology.the morphological and physiological characteristics of 20 strains of motile, gram-negative, yellow-pigmented oxidative bacilli (groups ve-1 and ve-2) isolated in clinical bacteriology are described. electron micrographs demonstrate the polar multitrichous flagella of group ve-1 and polar monotrichous flagella of group ve-2. data obtained from guanine plus cytosine ratio studies of 56.8% for ve-1 and 68.9% for ve-2 distinguish the two groups of bacteria.19751176609
studies on the mechanism of lipase reaction. i. inhibition of lipase activity by emulsion of organic solvents. 19751182871
studies on the lipase of chromobacterium viscosum. iv. substrate specificity of a low molecular weight lipase. 19751182872
identification of chromobacterium violaceum: pigmented and non-pigmented strains.the classification and, therefore, identification of chromobacterium violaceum has been based upon its ability to produce a violet pigment. although the organism may yield non-pigmented variants when subcultured on artificial media, the isolation of non-pigmented strains from pathological tissues or from nature had not been reported. with a method established for the identification of c. violaceum regardless of violet pigmentation, non-pigmented strains were isolated from nature. the presence of ...19751236933
unusual chromobacterium violaceum: aerogenic strains.this is the first report of chromobacterium violaceum strains that are aerogenic. two pigmented strains of c. violaceum and their nonpigmented variants produced gas in glucose broth. kliger iron agar, and triple sugar iron agar. they were similar morphologically and biochemically to other anaerogenic strains studied previously.19761254704
morphological, physiological, and biochemical characteristics of some violet-pigmented bacteria isolated from seawater.sixteen violet-pigmented heterotrophic bacilli were isolated from mediterranean coastal waters. morphological and physiological studies showed that they have a number of characteristics specific to the genus chromobacterium. however, the absence of catalase, the presence of oxidase, and, more especially, the low percentage of bases guanine and cytosine in their dna exclude them from the genus. the specificity of some characters assigned to chromobacterium can thus be discussed. several features ...19761260521
formation of indole-3-carboxylic acid by chromobacterium violaceum.l-tryptophan is converted to indole-3-carboxylic acid by growing cultures and resting cell suspensions of chromobacterium violaceum19761262308
absolute configuration of 3-hydroxy fatty acids present in lipopolysaccharides from various bacterial groups.the absolute configuration of 3-hydroxy fatty acids has been studied, which are present in the lipopolysaccharides of the following bacteria: phodopseudomonas gelatinosa, rh. viridis, rhodospirillum tenue, chromobacterium violaceum, pseudomonas aeruginosa, bordetella pertussis, vibrio metchnikovii, vibrio cholerae, salmonella spp., escherichia coli, shigella flexneri, proteus mirabilis, yersinia enterocolitica and fusobacterium nucleatum. the 3-hydroxy acids were liberated by strong alkaline hyd ...19761278168
modulation of endotoxin-induced monokine release in human monocytes by lipid a partial structures that inhibit binding of 125i-lipopolysaccharide.we have previously shown that the synthetic tetraacyl precursor ia (compound 406, la-14-pp, or lipid iva) was not able to induce the production of tumor necrosis factor, interleukin-1, and interleukin-6 in human monocytes but strongly antagonized lipopolysaccharide (lps)-induced formation of these monokines. this inhibition was detectable at the level of mrna production. to achieve a better understanding of molecular basis of this inhibition, we investigated whether lipid a precursor ia (la-14-p ...19921280625
bacterial chemistry. vi. biological activities and cytotoxicity of 1,3-dihydro-2h-indol-2-one derivatives.the biosynthetic pigment from chromobacterium violaceum bb-78, 1,3-dihydro-2h-indol-2-one and its derivatives exhibit biological activities such as antimicrobial action, low hemolytic effects on red blood cells and in vitro trypanocide activity. a relatively high cytotoxicity on v-79 hamster fibroblast cells of the biosynthetic pigment was found, although with the methylol derivative the toxicity was almost eliminated. the methylol derivative exhibited similar toxicity as nifurtimox, a known, co ...19921341576
isolation and characterization of acetonitrile utilizing bacteria.bacteria utilizing high concentrations of acetonitrile as the sole carbon source were isolated and identified as chromobacterium sp. and pseudomonas aeruginosa. maximum growth was attained after 96 h of incubation and p. aeruginosa grew slightly faster than chromobacterium sp. the strains were able to grow and oxidize acetonitrile at concentrations as high as 600 mm. however, higher concentrations inhibited growth and oxygen uptake. degradation studies with (14c)acetonitrile indicated 57% of ace ...19901367463
kinetics and stability of a chromobacterium viscosum lipase in reversed micellar and aqueous media.the lipolytic activity of chromobacterium viscosum lipase b (ec; triacylglycerol hydrolase) solubilized both in water and aot/isooctane reversed micelles has been investigated using triolein as a substrate. the influence of relevant parameters in the catalytic activity such as temperature, ph, surfactant and substrate concentrations, and water content was tested and compared in both media. a study of stability of the lipase was carried out, with particular reference to the influence of ...19921368011
high resolution analysis of carotenoids in human plasma by high-performance liquid chromatography. 19921435309
stereoselectivity of lipases: esterification reactions of octadecylglycerol.stereoselectivity of several triacylglycerol lipases (ec has been investigated in the enzymatic esterification of rac-1-o-octadecylglycerol with oleic acid in the presence of organic solvents, such as hexane. x-1(3)-o-octadecylmonooleoylglycerols were the only products formed with most lipases; considerable proportions of x-1(3)-o-octadecyldioleoylglycerols were also formed with the lipase from candida cylindracea. the mixtures of unesterified enantiomeric substrates, i.e., x-1(3)-o-oct ...19921511492
fatal chromobacterium violaceum infections associated with exposure to stagnant waters. 19921528650
chemical and cultural characterization of cdc group wo-1, a weakly oxidative gram-negative group of organisms isolated from clinical sources.ninety-six strains of weakly oxidative gram-negative rods isolated primarily from clinical specimens form a distinct group that has been designated centers for disease control (cdc) group wo-1 (wo stands for weak oxidizer). the phenotypic characteristics of cdc group wo-1 were most similar to those of comamonas acidovorans, pseudomonas mallei, and cdc pink coccoid group iii. the wo-1 group can be differentiated from c. acidovorans by the oxidation of glucose (often weak and sometimes delayed), m ...19921537895
identification of a neutral lipid core in a transiently expressed and secreted lipoprotein containing an apob-48-like apolipoprotein.the presence of core lipids in lipoproteins expressed and secreted by transfected hepg2 cells was demonstrated by measuring the densities of these lipoproteins before and after treatment with a bacterial lipase specific for neutral lipids. hepg2 cells were reproducibly transfected with prsv/b48, containing a truncated human apolipoprotein b-100 (apob-100) cdna (nucleotides 1 to 6860, where nucleotide 129 is the start of translation). northern blots of cellular message probed with apob-48 showed ...19921569375
x-ray absorption studies of the cu-dependent phenylalanine hydroxylase from chromobacterium violaceum. comparison of the copper coordination in oxidized and dithionite-reduced enzymes.the coordination chemistry of the cu sites of phenylalanine hydroxylase (pah) from chromobacterium violaceum has been studied by x-ray absorption spectroscopy (xas). the exafs of the cu(ii) form of the enzyme resembles that of other non-blue copper proteins such as plasma amine oxidases and dopamine-beta-hydroxylase and is characteristic of a mixed n/o coordination shell containing histidine ligation. detailed simulations of the raw exafs data have been carried out using full curved-wave restrai ...19921606153
chromobacterium violaceum septicaemia: the intensive care management of two cases.the first human infection with chromobacterium violaceum was recorded in 1927, but since then there have been about 33 cases reported worldwide, including two from australia. chr. violaceum occurs in the tropics and subtropics and is generally considered to be nonpathogenic, but infection can occur in patients who are immunosuppressed and it has a high mortality rate. this paper presents the intensive care management of two cases of chr. violaceum infection occurring in far north queensland. the ...19921609950
metabolites formed during anaerobic transformation of toluene and o-xylene and their proposed relationship to the initial steps of toluene mineralization.strain t1 is a facultative bacterium that is capable of anaerobic toluene degradation under denitrifying conditions. while 80% of the carbon from toluene is either oxidized to carbon dioxide or assimilated into cellular carbon, a significant portion of the remainder is transformed into two dead-end metabolites. these metabolites were produced simultaneous to the mineralization of toluene and were identified as benzylsuccinic acid and benzylfumaric acid. identification was based on comparison of ...19921610173
cloning and expression of chromobacterium violaceum phenylalanine hydroxylase in escherichia coli and comparison of amino acid sequence with mammalian aromatic amino acid hydroxylases.the complete amino acid sequence (296 amino acids) of chromobacterium violaceum phenylalanine hydroxylase (pah) was determined by nucleotide analysis of a dna clone isolated using both a synthetic oligonucleotide probe based on the nh2-terminal amino acid sequence and an antibody against this enzyme. the apal i fragment (approximately 1.9 kilobase pairs) containing the entire pah gene was subcloned in pbluescript ii and induced by isopropyl-beta-d-thiogalactopyranoside. in order to eliminate fus ...19911655752
reverse enzyme synthesis in microemulsion-based organo-gels.lipase from three different sources has been immobilised in microemulsion-based gels (mbgs) with retention of catalytic activity. such lipase-containing mbgs prove to be novel solid-phase catalysts for use in apolar organic solvents such as n-heptane. using these systems, preparative-scale synthesis of a wide variety of esters under mild conditions was possible with products easily isolated and obtained in high yield. stereoselective esterification of octan-2-ol was observed for all three lipase ...19911707672
[first isolation of chromobacterium violaceum strains, also achromogenic, in drinking water]. 19901715722
survival of the fish pathogen aeromonas salmonicida in seawater.survival of aeromonas salmonicida in natural (non-sterile) seawater, as determined from colony counts on marine agar, was found to be influenced by the presence of potentially inhibitory organisms, i.e., acinetobacter, aeromonas hydrophila, chromobacterium, escherichia coli, flavobacterium and pseudomonas, and their metabolites. yet, samples, thought to be devoid of culturable a. salmonicida, were found to contain cells, which were filterable through 0.22 and 0.45 microns millipore millex porosi ...19911769545
beta-d-glucuronidase (bdg) activity of gram-negative bacteria.bdg is an inducible enzyme that is encoded by the uida gene in escherichia coli. genetic sequences of this gene are present in most if not all e. coli strains regardless of the bdg phenotype. expression of bdg activity can be influenced by lactose-induced catabolite repression or genetic mutations. salmonella, shigella and yersinia strains frequently exhibit positive bdg reaction. bdg activity of strains belonging to genus edwardsiella, serratia, yersinia, vibrio, erwinia, alcaligenes, acinetoba ...19911817425
solution behaviour of chromobacter viscosum and pseudomonas sp. lipases. no evidence of self-association.1. the size of two bacterial lipases was studied by sds/page, sedimentation velocity and sedimentation equilibrium to test for possible self-association behaviour. 2. mr values of selected lipases were obtained from sds/page and sedimentation-velocity measurements, together with an absolute determination by sedimentation equilibrium 3. the mr values obtained in a variety of aqueous solvents indicate that lipases do not self-associate in solution, suggesting the absence of surface hydrophobic pat ...19911996958
hydrodynamic characterization of chromobacter viscosum lipase. 19902083651
endotoxin size in hemodialysis solutions: modifications in presence of concentrated salt solutions and bacterial products. 19902183572
comparative genetic organization of incompatibility group p degradative plasmids.plasmids that encode genes for the degradation of recalcitrant compounds are often examined only for characteristics of the degradative pathways and ignore regions that are necessary for plasmid replication, incompatibility, and conjugation. if these characteristics were known, then the mobility of the catabolic genes between species could be predicted and different catabolic pathways might be combined to alter substrate range. two catabolic plasmids, pss50 and pss60, isolated from chlorobipheny ...19902254257
the use of tween 20 in a sensitive turbidimetric assay of lipolytic enzymes.a turbidimetric esterase assay was developed using a tween 20 solution in the presence of cacl2 and lysobacter enzymogenes esterase (ec as the enzyme source. the reaction was followed by measuring the increase in the optical density at 500 nm (od500) due to the hydrolytic release of the fatty acids from tween 20 and their precipitation as the calcium salts. concentrations of 1.8% tween and 3 mm cacl2 were found to be optimal for the assay of 0.036 to 0.15 esterase units in a 4-ml reacti ...19892501015
pneumonia and meningitis caused by a new nonfermentative unknown gram-negative isolates of an unclassified bacterium resembling flavobacterium spp. were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, protein profile analysis, and serologic data. the unclassified isolates were differentiated from flavobacterium meningosepticum, flavobacterium odoratum, flavobacterium balustinum, flavobacterium strain iib, chromobacterium violaceum, aquaspirillum serpens, and pseudomonas spp. the bacterium ...19892504766
bacterial chemistry-iii: preliminary studies on trypanosomal activities of chromobacterium violaceum has been found that the pigment-i from chromobacterium violaceum, 3-[1,2-dihydro5-(5-hydroxy-1h-indol-3-yl)-2-oxo-3h-pyrrol-3-yli diene] 1,3-dihydro-2h-indol-2-one, has trypanocide activity. the formylated derivatives, pigment-iii, immobilized 100% of the trypanosoma cruzi at a level of 46 microm after 48 h of interaction with a total growth inhibition in the same period. pigment-i exhibited low toxicity and a dna synthesis inhibition similar to that of nifurtimox, a known trypanocide compoun ...19892512826
bacterial ethylene synthesis from 2-oxo-4-thiobutyric acid and from methionine.the ability of selected bacterial cultures to synthesize ethylene during growth in nutrient broth supplemented with methionine or 2-oxo-4-methylthiobutyric acid (kmba) was examined. although most cultures transformed kmba into ethylene, only those of escherichia coli spao and chromobacterium violaceum were able to convert exogenously added methionine to ethylene. in chemically defined media, e. coli spao produced the highest amounts of ethylene from methionine and kmba. this capability was affec ...19892559143
temperature-dependent incorporation of 4-amino-l-arabinose in lipid a of distinct gram-negative bacteria.the presence and the relative amount of 4-amino-l-arabinose in lipopolysaccharides of members of the enterobacteriaceae family and in a single strain of chromobacterium violaceum has been studied with regard to growth-temperature dependent variations. changes in the presence and the amount of 4-amino-l-arabinose (4-aa) were observed in almost all cases, but the variations observed were not consistent among different species. while salmonella minnesota and proteus mirabilis showed higher levels o ...19892583477
bacterial colonization of domestic reverse-osmosis water filtration units.we have analyzed the bacterial content of water from the reservoirs of 300 reverse-osmosis units installed in households. the heterotrophic plate counts on r2a medium (20 and 35 degrees c) ranged from 0 to 10(7) colony forming units per millilitre (cfu/ml). most reservoirs contained water with bacterial counts between 10(4) and 10(5) cfu/ml. the bacteria identified were pseudomonas (not aeruginosa), alcaligenes or moraxella, acinetobacter, flavobacterium, and chromobacterium. this report emphasi ...19892611732
effect of growth conditions on production of violacein by chromobacterium violaceum (bb-78 strain).1. chromobacterium violaceum (strain bb-78 isolated in brazil) produces violacein, a substance potentially useful in phototherapy and with antibiotic and trypanocide activity. culture conditions were optimized for the production of violacein and changes in nutrients, temperature and ph were correlated to cellular growth. 2. methionine was the only absolute requirement for growth. alanine, arginine, tryptophan and vitamin b12 stimulated growth and the microorganism utilized both the d and l forms ...19892620169
the pattern of protein synthesis induced by heat-shock of the moderately halophilic bacterium chromobacterium marismortui: protective effect of high salt concentration against the thermal shock.the protein synthetic response to heat shock of the moderately halophilic bacterium chromobacterium marismortui was examined. upon exposure to elevated temperature there is an increased synthesis of a specific subset of proteins (heat shock proteins-hsps) in the molecular weight region of 15 to 90 kd, while normal protein synthesis is severely repressed. the synthesis of hsps reaches a maximum 5 min after heat shock at 42 degrees c. cells recovered their normal protein synthesis patterns rapidly ...19892716536
mechanistic studies on phenylalanine hydroxylase from chromobacterium violaceum. evidence for the formation of an enzyme-oxygen complex.steady-state kinetic analysis of pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum indicated that the enzyme follows a partially ordered reaction mechanism. the data suggested that oxygen is the first substrate to bind to the enzyme. this result was further supported by rapid-quench experiments in which the enzyme-oxygen complex was trapped to yield product. additional support for the presence of an enzyme-oxygen complex was derived from magnetic susceptibility measuremen ...19892719947
near-fatal septicaemia with chromobacterium violaceum.chromobacterium violaceum is a gram-negative organism which normally inhabits water and soil. human infection is unusual and is associated with a high mortality rate. we describe a typical case of disseminated infection with chr. violaceum in a male carpet cleaner. the possible origin and treatment of the infection is discussed.19892733620
an acute pleuropneumonia in a pig caused by chromobacterium violaceum.a 2.5-month-old, 30 kg duroc pig died 10 days after showing clinical signs of dyspnoea and diarrhoea. acute necrotizing and fibrinous pleuropneumonia with locally extensive lesions was found. chromobacterium violaceum was isolated from pneumonic lung tissues and intratracheal inoculation of a pure culture into two spf pigs reproduced lesions similar to those found in the natural infection.19892760278
occurrence of drug-resistant bacteria in communal well water around port harcourt, nigeria.a total of 108 raw water samples was collected from 36 wells at nine shanty settlements around port harcourt, nigeria, over a period of 7 months. samples were analysed for their bacteriological quality. selected bacterial strains isolated from the samples were tested for their susceptibility to ten commonly used antibiotics. the organisms isolated include pseudomonas spp., klebsiella spp., staphylococcus spp., proteus spp., enterococcus faecalis, aeromonas spp., escherichia coli, chromobacterium ...19892776852
adduct formation between the cupric site of phenylalanine hydroxylase from chromobacterium violaceum and 6,7-dimethyltetrahydropterin.the interaction of pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum with the cofactor analogue 5-deaza-6-methyltetrahydropterin and the cofactor 6,7-dimethyltetrahydropterin (dmph4) has been investigated by multifrequency electron spin resonance (esr) spectroscopy. 5-deaza-6-methyltetrahydropterin, which lacks the n-5 nitrogen present in the pyrazine ring of dmph4, binds tightly to the cupric form of the enzyme; however, no changes are observed in the esr parameters of t ...19872822093
a comparative study of virulent and avirulent strains of chromobacterium violaceum.a clinical isolate and a soil isolate of chromobacterium violaceum were compared to determine differences in virulence-related characteristics. purified lipopolysaccharide (endotoxin) from the virulent, clinical strain was more reactive than that from the avirulent soil strain as determined by the limulus amebocyte lysate assay. there were no differences in hemolysin or cyanide production between the two strains. the virulent strain was more resistant to phagocytosis and intracellular killing by ...19882843271
partial purification and characterization of adp sulfurylase from the purple sulfur bacterium thiocapsa roseopersicina.high activities of adp and atp sulfurylase were found in the soluble protein fraction of thiocapsa roseopersicina strain 6311 (dsm 219). adp sulfurylase was partially purified and characterized. it was a very labile soluble enzyme with a molecular weight of 250,000. the optimum ph was 7.5 and the optimal temperature 35 degrees c. under test conditions the apparent km values were determined to be 0.33 mm for adenylylsulfate and 13 mm for phosphate.19882855979
phenylalanine 4-monooxygenase from chromobacterium violaceum. 19872885717
the mechanism of action of phenylalanine hydroxylase. 19863014980
phenylalanine hydroxylase from chromobacterium violaceum is a copper-containing monooxygenase. kinetics of the reductive activation of the enzyme.pterin-dependent phenylalanine hydroxylase from chromobacterium violaceum contains a stoichiometric amount of copper (cu2+, 1 mol/mol of enzyme). electron paramagnetic resonance spectroscopy of the enzyme indicates that it is a type ii copper-containing protein. the oxidized enzyme must be reduced by a single electron to be catalytically active. dithiothreitol was found to be an effective reducing agent for the enzyme. electron paramagnetic resonance data and kinetic results indicate the formati ...19863024714
structural heterogeneity regarding local shwartzman activity of lipid a.the relation of chemical structure to local shwartzman activity of lipid a preparations purified by thin-layer chromatography from five bacterial strains was examined. two lipid a fractions from e. coli f515--ec-a2 and ec-a3--exhibited strong activity, similar to that of previous synthetic e. coli-type lipid a (compound 506 or la-15-pp). the ec-a3 fraction contained a component that appeared to be structurally identical to compound 506, and the main component of ec-a2 fraction was structurally s ...19883057331
comparison of the in vitro activity of ciprofloxacin and 24 other antimicrobial agents against clinical strains of chromobacterium violaceum.eleven clinical strains of chromobacterium violaceum were tested for their susceptibility to 25 antimicrobial agents. ciprofloxacin was the most active of the compounds tested although norfloxacin and pefloxacin were highly active. no resistance was detected to mezlocillin, piperacillin, apalcillin, imipenem, and aztreonam while a single strain was resistant to ticarcillin. among the cephalosporin/cephamycin group only cefotetan showed good in vitro activity. gentamicin was more active than amik ...19883168426
[the role of gram-negative non-fermenting bacteria in hospital infections]. 19883237527
occurrence, transfer and mobilization in epilithic strains of acinetobacter of mercury-resistance plasmids capable of transformation.a 7.8 kb plasmid (pqm17) encoding mercury resistance was isolated from two epilithic strains of acinetobacter calcoaceticus. the plasmid had a broad host range when mobilized by rp1, transferring into pseudomonas aeruginosa, p. putida, p. fluorescens, escherichia coli, proteus vulgaris and chromobacterium sp. with frequencies ranging from 5.3 x 10(-9) to 4.6 x 10(-4) per recipient. the plasmid could be transferred into a. calcoaceticus bd413 using intact cells of donor and recipient bacteria (i. ...19883254940
[chromobacterium violaceum infection. presentation of a case with fatal outcome]. 19863299554
natural microbiologic hazards of the aquatic environment. 19873311341
[role of halophilic and halotrophic bacteria in the chronic problems of long-term dialysis]. 19873319061
aerocavin, a new antibiotic produced by chromobacterium violaceum.a new antibiotic, aerocavin, has been isolated from fermentation broths of a non-pigmented strain of chromobacterium violaceum. the structure 1 was deduced from its spectroscopic properties and x-ray diffraction analysis. aerocavin exhibits activity in vitro against gram-positive and gram-negative bacteria.19883372350
aerocyanidin, a new antibiotic produced by chromobacterium violaceum.aerocyanidin, a new antibiotic containing an isonitrile group, has been isolated from fermentations of chromobacterium violaceum atcc 53434. structure 1 was assigned on the bais of spectroscopic characterization of the antibiotic and of a degradation product that results from treatment with base. the antibiotic is primarily active against gram-positive bacteria.19883372351
[use of chromobacterin to control the larvae of blood-sucking mosquitoes]. 19873448483
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