Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| genetic relatedness of bordetella species as determined by macrorestriction digests resolved by pulsed-field gel electrophoresis. | the genetic relatedness of the three medically important bordetella species was examined by macrorestriction digestion of dna with the rarely cutting enzyme xbai and resolution by pulsed-field gel electrophoresis. our data showed that bordetella pertussis, bordetella parapertussis, and bordetella bronchiseptica produced species-specific macrorestriction profiles and that there was some variation between different isolates of the same species. conserved bands at 130 and 155 kb occurred with b. pe ... | 1993 | 8240949 |
| purification and characterisation of haemagglutinin from bordetella bronchiseptica. | a surface protein of bordetella bronchiseptica was purified in one step by affinity chromatography with bovine submaxillary mucin coupled to agarose. the purified protein, with a mol. wt of 200 kda and an iso-electric point of pi 6.5, showed haemagglutinating activity for bovine erythrocytes. this haemagglutinin (ha) inhibited the adherence of b. bronchiseptica to a rat lung cell line (l2) and was able to bind to n-acetylneuraminic acid. these findings suggest that the ha of b. bronchiseptica is ... | 1993 | 8246255 |
| association of microbiologic flora with clinical, endoscopic, and pulmonary cytologic findings in foals with distal respiratory tract infection. | undifferentiated distal respiratory tract disease (nasal discharge, cough, pneumonia) in foals (1 to 8 months old) is a burdensome economic problem on breeding farms; yet, the infective agents associated with these episodes have not been well described. possible causes of these episodes of illness were investigated by culturing specimens of proximal and distal airways of clinically diseased foals (n = 101), prior to any treatment, for aerobic and anaerobic bacteria and viruses (rhinoviruses, equ ... | 1993 | 8250386 |
| comparison of three biological assays for the detection of toxigenic properties of bordetella bronchiseptica associated with atrophic rhinitis. | the toxigenic properties of bordetella bronchiseptica strains associated with clinical atrophic rhinitis were compared using three biological assays: the guinea pig skin test, the mouse spleen atrophy and the suckling mice mortality assay. forty one sick pigs were cultured and, in 20 of them b. bronchiseptica strains were isolated. eighteen of the strains were toxigenic by at least one test. however, positivity did not always coincide amongst assays. this discrepancy raises the possibility of ei ... | 1993 | 8273270 |
| an enzyme-linked immunosorbent assay (elisa) for monitoring guineapigs and rabbits for bordetella bronchiseptica antibodies. | an enzyme-linked immunosorbent assay (elisa) for monitoring antibodies specific to bordetella bronchiseptica in guineapigs and rabbits was developed. in conventional and spf colonies of guineapigs and rabbits, the elisa was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. the elisa showed a sensitivity of 100% and a specificity of 90% in guineapigs. in rabbits the sensitivity and specificity of the elisa were 97% and 91% respecti ... | 1993 | 8277707 |
| long-term survival of bordetella bronchiseptica in lakewater and in buffered saline without added nutrients. | bordetella bronchiseptica grew from small inocula, and retained viability for at least 24 weeks, in unsupplemented lakewater or phosphate-buffered saline. from washed inocula of around 10(3) colony-forming units/ml, there was growth at both 10 degrees c and 37 degrees c to give 10(6)-10(7) colony-forming units/ml. at 10 degrees c, these counts were maintained with little diminution up to week 24 when observations ceased. in the tests at 37 degrees c, two of three strains tested showed similar re ... | 1993 | 8319892 |
| phylogenetic position of taylorella equigenitalis determined by analysis of amplified 16s ribosomal dna sequences. | the 16s ribosomal dna sequence of taylorella equigenitalis (formerly haemophilus equigenitalis), the causative organism of contagious equine metritis, was determined. a phylogenetic analysis of this sequence revealed a phylogenetic position of t. equigenitalis in the beta subclass of the class proteobacteria apart from the position of haemophilus influenzae, which belongs to the gamma subclass of proteobacteria. a close phylogenetic relationship among t. equigenitalis, alcaligenes xylosoxidans, ... | 1993 | 8347520 |
| streptococci and pasteurella spp. associated with disease of the equine lower respiratory tract. | the likelihood of finding evidence of inflammation in 551 tracheal washes collected endoscopically from 278 thoroughbred racehorses increased with the number of bacterial colony forming units (cfu) per ml of wash (p < 0.001). the aerobic bacteria streptococcus zooepidemicus, pasteurella/actinobacillus-like species and streptococcus pneumoniae were significantly associated with lower airway inflammation whereas coagulase-negative staphylococcus spp., alpha-haemolytic streptococcus spp., acinetoba ... | 1993 | 8354218 |
| stimulation of dna synthesis in osteoblast-like mc3t3-e1 cells by bordetella bronchiseptica dermonecrotic toxin. | we investigated the effects of bordetella bronchiseptica dermonecrotic toxin on dna synthesis in mc3t3-e1 cells. the rate of [methyl-3h]thymidine incorporation increased in the toxin-treated cells more than 24 h after addition of the toxin under the serum-starved conditions. this effect was dependent on the toxin concentration ranging from 0.3 to 3 ng/ml and was eliminated by aphidicolin and hydroxyurea, inhibitors for dna replication. in the toxin-treated culture, the number of cells did not in ... | 1993 | 8359884 |
| isolation and characterization of bordetella bronchiseptica mutants deficient in siderophore activity. | iron acquisition by the gram-negative pathogens bordetella bronchiseptica and bordetella pertussis is thought to occur by hydroxamate siderophore-mediated transport as well as an apparently siderophore-independent process by which host transferrins bind to bacterial surface receptors. we constructed b. bronchiseptica mutants deficient in siderophore activity by insertional mutagenesis with minitn5/lacz1. the mutants could be placed into four distinct complementation groups, as determined from cr ... | 1993 | 8381782 |
| effect of inflammation on peripheral airway reactivity in dogs. | 1. eight mongrel dogs were studied to evaluate the effect of local inflammation on the response of collateral airways to histamine. in each dog we measured the baseline collateral resistance and its response to increasing doses of nebulized histamine. these measurements were obtained at weekly intervals, twice under control conditions and in three subsequent weeks after a local instillation of the bacteria bordetella bronchiseptica. on each study day, after the measurements of collateral resista ... | 1993 | 8382138 |
| interaction between immunity to bordetella bronchiseptica and infection of pig herds by bordetella bronchiseptica and pasteurella multocida. | the dynamics of toxigenic bordetella bronchiseptica and pasteurella multocida infection and the b. bronchiseptica specific antibody content of the blood and nasal secretion were studied in three hungarian and three dutch pig herds. in both countries, the studies involved young sows that had farrowed once or twice (ys), old sows that had farrowed more than four times (os), and their piglets. the results indicate that dutch sows are characterized by a lower prevalence of b. bronchiseptica and p. m ... | 1993 | 8399743 |
| virulence of bordetella bronchiseptica: role of adenylate cyclase-hemolysin. | bordetella bronchiseptica is a pathogen of laboratory, domestic, and wild animals and sometimes of humans. in the present study some characteristics of the virulence of b. bronchiseptica isolates of different origin were studied. all isolates had similar phenotypes, similar bacteriological characters, and synthesized adenylate cyclase-hemolysin, filamentous hemagglutinin and pertactin but not pertussis toxin. these isolates, however, differed in their ability to express dermonecrotic toxin and t ... | 1993 | 8406794 |
| nasal epithelial changes induced in piglets by acetic acid and by bordetella bronchiseptica. | research on atrophic rhinitis of pigs has shown that both bordetella bronchiseptica infection and experimental treatment with acetic acid predispose the nasal mucosa to colonization with pasteurella multocida. gnotobiotic piglets aged 3 days were dosed intranasally with either b. bronchiseptica (n = 6) or acetic acid 1 per cent (n = 10) and killed at intervals up to the 4th day after treatment. samples of the ventral turbinates were examined by light microscopy and scanning and transmission elec ... | 1993 | 8408782 |
| [the effectiveness of tilmicosin in respiratory diseases of swine]. | within the scope of the clinical evaluation of tilmicosin in enzootic pneumonia of pigs, nasal swabs from 78 animals were taken, before and after oral medication of different doses (0, 100, 200, 300 mg tilmicosin/kg dry food), and examined bacteriologically for pasteurella multocida, bordetella bronchiseptica und haemophilus parasuis. the bacteria aforementioned were isolated from the nasal secretions of 83% of the pigs, 14 days after crowding without any prophylactic regime. it could be demonst ... | 1993 | 8431202 |
| comparison of isolation methods for the recovery of bordetella bronchiseptica and pasteurella multocida from the nasal cavities of piglets. | nasal swabs from 241 piglets from 12 herds with clinical atrophic rhinitis and 283 piglets from 14 herds without clinical atrophic rhinitis were examined for the presence of bordetella bronchiseptica and/or pasteurella multocida. for b. bronchiseptica, swabs were streaked on three selective media. blood agar supplemented with cephalexin was the most satisfactory selective culture medium for the isolation of b. bronchiseptica. for p. multocida, swabs were also streaked on three selective media. m ... | 1993 | 8432824 |
| bordetella bronchiseptica pneumonia in a koala. | a 3-year-old male koala (phascolarctos cinereus) was evaluated because of severe acute respiratory distress. despite medical treatment, the koala died. gross and histologic lesions consistent with acute bacterial pneumonia were identified at necropsy and bordetella bronchiseptica was isolated from pulmonary tissue. there are 2 prior reports from australia of fatal b bronchiseptica pneumonia in captive koalas. | 1993 | 8468231 |
| functional analysis of the cya promoter of bordetella pertussis. | the cyaa gene of bordetella pertussis and of bordetella bronchiseptica encodes a toxin which is a bifunctional protein exhibiting adenylate cyclase and haemolytic activities. in bordetella, virulence factors are synthesized under the control of the bvg regulatory locus, in response to environmental signals. in escherichia coli the cyaa gene is not expressed, nor is it activated by bvg indicating that the activation of cya by bvg is indirect. to characterize cis-acting regulatory regions required ... | 1993 | 8469114 |
| a siderophore production mutant of bordetella bronchiseptica cannot use lactoferrin as an iron source. | bordetella bronchiseptica secreted a hydroxamate siderophore when grown in fe-depleted medium. a tn5lac insertion mutant of b. bronchiseptica, dbb22, did not produce this hydroxamate siderophore and was incapable of using lactoferrin as an fe source. our data suggest that b. bronchiseptica uses a siderophore for removal of fe from lactoferrin and transferrin rather than relying upon a receptor for these host fe-binding proteins. | 1993 | 8500910 |
| flagellin gene transcription in bordetella bronchiseptica is regulated by the bvgas virulence control system. | the products of the bvgas locus activate expression of a majority of the known bordetella virulence factors but also exert negative control over a class of genes called vrg genes (bvg-repressed genes). bvgas negatively controls the production of flagella and the phenotype of motility in bordetella bronchiseptica. in this study flaa, the flagellin gene, was cloned and characterized to facilitate studies of this negative control pathway. an internal flaa probe detected hybridizing sequences on gen ... | 1993 | 8501051 |
| isolation of bordetella bronchiseptica from a patient with aids. | 1993 | 8513824 | |
| [features of expression of the pertussis toxin operon under the control of its own and heterologous promotors in bordetella bronchiseptica cells]. | expression of the cloned operon encoding the pertussis toxin synthesis under the control of operons own vir-dependent promoter or vir-independent promoter of escherichia coli origin was studied. proteins produced by the recombinant strains have been characterized. the pertussis toxin operon was shown to express under the control of both homologous and heterologous promoters in bordetella bronchiseptica cells. use of the lac-promoter increases the yield of produced toxin twofold. copy number of o ... | 1993 | 8515746 |
| the ornithine decarboxylase gene odc is required for alcaligin siderophore biosynthesis in bordetella spp.: putrescine is a precursor of alcaligin. | chromosomal insertions defining bordetella bronchiseptica siderophore phenotypic complementation group iii mutants brm3 and brm5 were found to reside approximately 200 to 300 bp apart by restriction mapping of cloned genomic regions associated with the insertion markers. dna hybridization analysis using b. bronchiseptica genomic dna sequences flanking the cloned brm3 insertion marker identified homologous bordetella pertussis ut25 cosmids that complemented the siderophore biosynthesis defect of ... | 1996 | 8550442 |
| bordetella bronchiseptica pneumonia in an aids patient: a new opportunistic infection. | 1995 | 8557395 | |
| identification of alca, a bordetella bronchiseptica gene necessary for alcaligin production. | the alca gene, essential for the production of the dihydroxamate siderophore, alcaligin, by bordetella bronchiseptica, was cloned and sequenced. the alca gene was identified on a 4.7-kb ecori genomic fragment adjacent to a tn5lac transposon insertion that inactivated alcaligin production in strain mbord846. analysis of the alca nucleotide sequence revealed a putative fur-binding site, suggesting that expression of this gene is repressed by iron. the deduced amino-acid sequence of this open readi ... | 1995 | 8566764 |
| nonradioactive colony lift-hybridization assay for detection of bordetella bronchiseptica infection in swine. | current methods for the isolation and identification of bordetella bronchiseptica from clinical samples are time-consuming and are based, in part, on subjective observations. we describe the use of a bordetella-specific dna probe in a nonradioactive colony lift-hybridization assay for the identification of b. bronchiseptica. eleven of 82 clinical specimens were found to contain b. bronchiseptica by this method, while only 5 of these were reported to contain the organism when the specimens were a ... | 1995 | 8567904 |
| a bordetella pertussis fepa homologue required for utilization of exogenous ferric enterobactin. | the bfea (bordetella ferric enterobactin) receptor gene was cloned from a bordetella pertussis chromosomal library by using a screen in escherichia coli to detect iron-repressed genes encoding exported proteins translationally fused to the e. coli phoa gene. the bfea gene encoded a protein with a molecular mass of approximately 80 kda and about 50% amino acid sequence identity to both the fepa- and pfea-encoded enterobactin receptors of e. coli and pseudomonas aeruginosa, respectively. enterobac ... | 1995 | 8574411 |
| effects of bordetella bronchiseptica dermonecrotizing toxin on bone formation in calvaria of neonatal rats. | the effects of bordetella bronchiseptica dermonecrotizing toxin on bone formation were investigated using a purified toxin preparation. single injection of 4.3 ng of the toxin into the subcutaneous tissue overlying the calvariae of neonatal rats necrotized periosteum of parietal bone and degenerated osteoblasts within two days. nine days after the injection, the lesion of the bone tissue became severe; the bone matrix became thin and fragmented. these observations indicate that dermonecrotizing ... | 1995 | 8580898 |
| intranasal inoculation of bordetella bronchiseptica in mice induces long-lasting antibody and t-cell mediated immune responses. | humoral and cellular immune responses were analysed in mice inoculated intranasally with bordetella bronchiseptica. after infection, the number of bacteria that colonized the respiratory tract of the mice increased during the first day and decreased thereafter. total igg levels increased as early as 14 days after infection and decreased with time after infection, whereas total iga and igm levels were lower but remained stable. specific antibodies to the bacteria were mainly igg2a and iga and per ... | 1996 | 8633198 |
| [are nasal swabs for swine appropriate for the diagnosis of bacterial pneumonia agents?]. | nasal swabs and lungs of 150 pigs with pneumonia were tested by culture at post mortem examination. the isolated agents were pasteurella multocida (p.m.), p. haemolytica, bordetella bronchiseptica, actinobacillus pleuropneumoniae, staphylococcus aureus, streptococcus spp. and escherichia coli. p.m. was most frequently found, and this agent only showed a significant correlation between lungs and nasal swabs. in 80.6% of pigs with p.m. in the lung the agent was detected in the nose, too. drug resi ... | 1995 | 8647001 |
| bordetella bronchiseptica infections in cats. | eleven cats from 10 different households or catteries were diagnosed with bordetella bronchiseptica (b. bronchiseptica) infections. seven cases proved fatal, in which b. bronchiseptica pneumonia was a primary or significant cause of death. the majority of cases (seven of 11) were in kittens less than eight weeks old. a universal complaint was coughing. bacterial cultures from transtracheal washes or lung tissue were successful in diagnosing bordetellosis in all cases. this report indicates that ... | 1996 | 8680922 |
| studies on natural transmission of bordetella bronchiseptica in cats. | two pregnant specific-pathogen-free (spf) cats, from a colony endemically infected with bordetella bronchiseptica, were housed under barrier conditions in the latter stages of pregnancy in order to study the possible transmission of the organism to their kittens. both queens had antibody titres to bordetella bronchiseptica of 1 in 320 at the start of the experiment but did not shed bordetella bronchiseptica until 9 days and 6 weeks after parturition, respectively. five of the nine kittens had de ... | 1996 | 8701574 |
| mutual viral and bacterial infections after housing rats of various breeders within an experimental unit. | fifteen athymic rat strains from 11 breeding colonies were housed within an experimental facility for an immunological study. health status records supplied with 14 of the strains listed infections by kilham's rat virus (krv), clostridium piliforme (bacillus piliformis) and pasteurella pneumotropica for 2, 2 and 1 colonies respectively. in sera taken previous to the study from euthymic rats of 10 strains, antibodies to krv were detected in 3 strains, to pneumonia virus of mice (pvm), rat corona ... | 1996 | 8709572 |
| [demonstration of pneumonia in swine as a constant problem: culture and immunofluorescence microscopic studies of bronchioalveolar lavage (bal) and serological findings]. | bronchoalveolar lavage was performed in 128 pigs from five fattening units showing acute pneumonia (48 animals), subclinical purulent pneumonia (17 animals), and chronic purulent pneumonia (63 animals). these samples were investigated for bacteria. additionally immunofluorescence microscopy as well as serological investigations were performed to detect antibodies against several bacteria and viruses. pasteurella multocida could be detected in more than a half of the samples of pigs with acute pn ... | 1996 | 8721303 |
| determination of the lifespan of erythrocytes from greyhounds, using an in vitro biotinylation technique. | to determine the rbc lifespan of greyhounds, using an in vitro labeling technique. | 1996 | 8723892 |
| purification, spectroscopic analysis and biological activity of the macrocyclic dihydroxamate siderophore alcaligin produced by bordetella pertussis and bordetella bronchiseptica. | hydroxamate siderophores of virulent bordetella pertussis and bordetella bronchiseptica strains were purified using a simple large-scale isolation procedure, and identified by various spectroscopic techniques as the macrocyclic dihydroxamate siderophore trivially known as alcaligin, 1,8(s),11,18(s)- tetrahydroxy-1,6,11,16-tetraazacycloeicosane-2,5,12,15-tetrone+ ++, which was previously isolated from the taxonomically-related bacterial species alcaligenes denitrificans subsp. xylosoxydans. alcal ... | 1996 | 8744901 |
| identification and characterization of iron-regulated bordetella pertussis alcaligin siderophore biosynthesis genes. | bordetella bronchiseptica mutants brm1, brm6, and brm9 fail to produce the native dihydroxamate siderophore alcaligin. a 4.5-kb bamhi-smal bordetella pertussis genomic dna fragment carried multiple genes required to restore alcaligin production to these siderophore-deficient mutants. phenotypic complementation analysis using subclones of the 4.5-kb genomic region demonstrated that the closely linked brm1 and brm9 mutations were genetically separable from the brm6 mutation, and both insertions ex ... | 1996 | 8759851 |
| the differentiation of bordetella parapertussis and bordetella bronchiseptica from humans and animals as determined by dna polymorphism mediated by two different insertion sequence elements suggests their phylogenetic relationship. | we describe a novel insertion sequence (is) element, is1002, which was found to be closely related to is481, which is found only in bordetella pertussis; we found that these two is elements have a level of sequence identity of 61.5% and also have almost identical terminal inverted repeats. is1002 was present in both b. pertussis and bordetella parapertussis strains isolated from humans. in contrast, is1002 was absent from b. parapertussis strains isolated from sheep. a dna fingerprint analysis p ... | 1996 | 8782670 |
| the quantitation of turbinate atrophy in pigs to measure the severity of induced atrophic rhinitis. | the two-fold purpose of this study was to establish a useful image analysis technique for quantitation of turbinate atrophy and to determine an optimum bacterial dose for inducing atrophic rhinitis (ar). two morphometric analysis methods were compared to determine a turbinate area ratio (tar) and a turbinate perimeter ratio (tpr); the ratios of turbinate area to total nostril area and of turbinate perimeter to total nostril perimeter, respectively. our first image analysis method differed from c ... | 1996 | 8785717 |
| serum haptoglobin concentration in growing swine after intranasal challenge with bordetella bronchiseptica and toxigenic pasteurella multocida type d. | the acute phase reaction, in association with progressive atrophic rhinitis (ar), was monitored for 3 wk using serum haptoglobin (hpt) quantification in thirty-six, 15 kg swine after intranasal challenge with varying doses of pasteurella multocida type d (toxigenic strain) and bordetella bronchiseptica. the challenge doses were administered alone or in combination with pigs divided into 9 isolated treatment groups. increasing doses of b. bronchiseptica were associated with lower serum hpt (p < 0 ... | 1996 | 8809387 |
| validation of nested bordetella pcr in pertussis vaccine trial. | a nested pcr, using a 239-bp sequence in the pertussis toxin promoter region, was developed and evaluated. the assay differentiates bordetella pertussis, bordetella parapertussis, and bordetella bronchiseptica by restriction enzyme analysis of the amplified fragments. the diagnostic performance of the pcr was evaluated in a swedish pertussis vaccine efficacy trial which took place from 1992 to 1995, including study children and household members and using culture and serology for laboratory conf ... | 1996 | 8815088 |
| the identification, cloning and mutagenesis of a genetic locus required for lipopolysaccharide biosynthesis in bordetella pertussis. | bordetella pertussis lipopolysaccharide (lps) is biologically active, being both toxic and immunogenic. using transposon mutagenesis we have identified a genetic locus required for the biosynthesis of lps in b. pertussis, which has been cloned and sequenced. we have also identified equivalent loci in bordetella bronchiseptica and bordetella parapertussis and cloned part of it from b. parapertussis. the amino acid sequences derived from most of the genes present in the sequenced b. pertussis locu ... | 1996 | 8821935 |
| evaluation of a pcr for detection of actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. | a pcr for the detection of actinobacillus pleuropneumoniae was evaluated. all of 102 field, isolates of a, pleuropneumoniae reacted in the pcr by amplification of a 985 bp product. no pcr amplification product was observed when examining strains of a. ureae, a. capsulatus, a. hominis, a. equuli, a, rossii, a. suis, escherichia coli, bordetella bronchiseptica. streptococcus suis, pasteurella haemolytica, pasteurella multocida, haemophilus parasuis, haemophilus taxon minor group, haemophilus taxon ... | 1996 | 8828126 |
| transfer of a pertussis toxin expression locus to isogenic bvg-positive and bvg-negative strains of bordetella bronchiseptica using an in vivo technique. | bordetella pertussis is the causative agent of whooping cough, a contagious childhood respiratory disease, increasing public concern over the safety of current whole-cell vaccines has led to decreased immunization rates and a subsequent increase in the incidence of the disease. the preparation of safer vaccines is at present concentrated on the production of detoxified virulence factors such as pertussis toxin (pt) for inclusion in acellular vaccine preparations. a permanently avirulent bordetel ... | 1996 | 8861392 |
| synergistic role of gaseous ammonia in etiology of pasteurella multocida-induced atrophic rhinitis in swine. | one-week-old large white piglets were weaned and allocated to 14 experimental groups, each composed of five animals. each group was housed in a separate rochester exposure chamber and exposed continuously to gaseous ammonia at either 0, 5, 10, 15, 25, 35, or 50 ppm (two groups per exposure level). one week after ammonia exposure commenced, the pigs from one group at each exposure level were inoculated intranasally with 9 x 10(7) cfu of pasteurella multocida type d. after a further 4 weeks of exp ... | 1996 | 8862582 |
| genetic regulation of airway colonization by bordetella species. | bordetella species are respiratory pathogens that infect humans and other animals. a majority of the virulence factors expressed by these bacteria are regulated by a master control locus, bvgas, a member of the two-component family of signal transduction systems. bvgas senses environmental signals and mediates signal transduction by a phosphorylation cascade that leads to a biphasic transition between the bvg+ and bvg- phases. from natural host studies using bordetella bronchiseptica, we have fo ... | 1996 | 8876534 |
| species selectivity of new siderophore-drug conjugates that use specific iron uptake for entry into bacteria. | siderophores selectively bind ferric iron and are involved in receptor-specific iron transport into bacteria. several types of siderophores were synthesized, and growth-promoting or inhibitory activities when they were conjugated to carbacephalosporin, erythromycylamine, or nalidixic acid were investigated. overall, 11 types of siderophores and 21 drug conjugates were tested against seven different bacterial species: escherichia coli, bordetella bronchiseptica, pasteurella multocida, pasteurella ... | 1996 | 8913474 |
| analysis of proteins encoded by the ptx and ptl genes of bordetella bronchiseptica and bordetella parapertussis. | bordetella pertussis is the only bacteria] species which is known to produce pertussis toxin (pt); however, both bordetella bronchiseptica and bordetella parapertussis contain regions homologous to the ptx genes of b. pertussis that encode the toxin subunits. after finding that several children with b. parapertussis infections exhibited modest antibody titers to pt, we examined the ptx genes of both b. parapertussis and b. bronchiseptica to determine whether they would encode stable, functional ... | 1996 | 8926063 |
| cloning, expression, and molecular characterization of the dermonecrotic toxin gene of bordetella spp. | a cosmid library of random fragments of bordetella bronchiseptica genomic dna was prepared and screened with oligonucleotides designed from the sequence of the b. pertussis dermonecrotic toxin (dnt) gene. two cosmid clones which apparently contained the complete b. bronchiseptica dnt gene were identified, but they did not express the toxin. a 5-kb fragment containing the dnt gene was subcloned from one of the cosmid clones onto a high-copy-number plasmid, and this resulted in low-level expressio ... | 1996 | 8926084 |
| molecular analysis of the bvg-repressed urease of bordetella bronchiseptica. | bordetella bronchiseptica is a ureolytic mammalian respiratory pathogen. we have investigated the regulation of urease in b. bronchiseptica and the potential role of this enzyme in eukaryotic invasion and intracellular survival. our results indicate urease is a bordetella virulence repressed gene. urease activity in virulent b. bronchiseptica bb7865 is up-regulated from basal levels by 5 gl1 magnesium sulphate at 37 degrees c. at 30 degrees c, urease activity remained at basal levels, even in th ... | 1996 | 8938644 |
| evidence for a ninth gene, ptli, in the locus encoding the pertussis toxin secretion system of bordetella pertussis and formation of a ptli-ptlf complex. | the pertussis toxin secretion system of bordetella pertussis initially was thought to comprise eight proteins, ptla-ptlh. we have investigated the existence of another protein, ptli, encoded by a putative gene located between ptld and ptle. a b. pertussis strain expressing a ptli::phoa translational fusion possessed alkaline phosphatase activity, suggesting that ptli encodes a protein. in b. pertussis, a protein with an apparent molecular weight of approximately 5,200 (similar to that predicted ... | 1996 | 8940184 |
| characterization of the fim2 and fim3 fimbrial subunit genes of bordetella bronchiseptica: roles of fim2 and fim3 fimbriae and flagella in adhesion. | with dna probes derived from the fimbrial subunit genes fim2 and fim3 of bordetella pertussis, two homologous subunit genes of bordetella bronchiseptica were identified and cloned. the nucleotide sequences of these genes were determined. comparison of these nucleotide sequences with the b. pertussis fimbrial fim2 and fim3 subunit genes showed a pronounced homology. therefore, the b. bronchiseptica genes were also designated fim2 and fim3. expression of the two b. bronchiseptica genes was demonst ... | 1996 | 8945552 |
| interpretive criteria for antimicrobial susceptibility testing of ceftiofur against bacteria associated with swine respiratory disease. | ceftiofur, an extended-spectrum cephalosporin, is active against a variety of animal pathogens, including organisms associated with swine respiratory disease. however, minimum inhibitory concentration (mic) breakpoint and disk diffusion interpretive criteria have not been established for swine pathogens. susceptibility tests were performed by broth microdilution mic and disk diffusion methods on 246 bacterial species that cause swine respiratory disease. ceftiofur was active against salmonella s ... | 1996 | 8953533 |
| comparative analysis of the virulence control systems of bordetella pertussis and bordetella bronchiseptica. | bordetella pertussis and bordetella bronchiseptica contain nearly identical bvgas signal-transduction systems that mediate a biphasic transition between virulent (bvg+) and avirulent (bvg-) phases. in the bvg+ phase, the two species express a similar set of adhesins and toxins, and in both organisms the transition to the bvg- phase occurs in response to the same environmental signals (low temperature or the presence of nicotinic acid or sulphate anion). these two species differ, however, with re ... | 1996 | 8971711 |
| microbiological study of transtracheal aspirates from dogs with suspected lower respiratory tract disease: 264 cases (1989-1995). | to determine the most commonly isolated bacterial species associated with lower respiratory tract disease of dogs and to determine susceptibility of these isolates to antimicrobial agents. | 1997 | 8977649 |
| stable expression of pertussis toxin in bordetella bronchiseptica under the control of a tightly regulated promoter. | pertussis toxin (pt) is an essential component of accellular vaccines against whooping cough. however, the industrial production of pt from bordetella pertussis is impaired by slow growth and poor yields. to overcome these problems, we have constructed a minitransposon containing the tox operon under the control of a tightly regulated promoter responsive to an aromatic inducer. the expression cassettes have been integrated into the chromosome of bordetella bronchiseptica 5376 and atcc 10580 bvg. ... | 1997 | 8979346 |
| [bordetella bronchiseptica in a patient with prostatic adenocarcinoma]. | 1996 | 8991453 | |
| non-motile mini-transposon mutants of bordetella bronchiseptica exhibit altered abilities to invade and survive in eukaryotic cells. | non-motile mutants of bordetella bronchiseptica were generated after mini-transposon mutagenesis. one non-motile mutant (designated vmm1) was derived from the bvg-positive strain bb7865 and four mutants (designated amm1-4) were derived from the isogenic bvg-negative strain bb7866. southern hybridisation analysis indicated that loss of motility was not due to the disruption of the flagellin subunit gene. western blot and transmission electron microscopic analysis indicated that three of the five ... | 1997 | 9011048 |
| bordetella bronchiseptica dermonecrotizing toxin, which activates a small gtp-binding protein rho, induces membrane organelle proliferation and caveolae formation. | we recently demonstrated that bordetella bronchiseptica dermonecrotizing toxin (dnt) modifies a small gtp-binding protein rho and causes assembly of actin stress fibers and focal adhesions in mc3t3-e1 cells, indicating that dnt activates the function of rho protein (horiguchi et al., 1995, j. cell sci. 108, 3243-3251). in this study, we examined by electron microscopy ultrastructural changes in dnt-treated mc3t3-e1 cells under conditions in which dnt elicited the above morphological changes. we ... | 1997 | 9013718 |
| an iron-regulated outer-membrane protein specific to bordetella bronchiseptica and homologous to ferric siderophore receptors. | the bfra (bordetella bronchiseptica ferric iron repressed outer-membrane protein) gene was cloned from bordetella bronchiseptica by screening a library of tnphoa insertion mutants for iron-repressed fusions to phoa. the bfra gene encoded an 80 kda outer-membrane protein with a high level of amino acid sequence identity to several bacterial proteins belonging to the family of ton b-dependent outer-membrane receptors. bfra was especially homologous to cir of escherichia coli, irga of vibrio choler ... | 1997 | 9025287 |
| role of bordetella bronchiseptica sialic acid-binding hemagglutinin as a putative colonization factor. | the role of bordetella bronchiseptica sialic acid-binding hemagglutinin (sbha) in bacterial colonization was studied by testing the ability of a phase-i strain carrying sbha and its spontaneous mutants deficient in sbha to colonize mouse tracheae. the mutants exhibited a remarkable reduction in colonizing ability, approximately 1,000-fold, when compared with that of the parent strain, suggesting that the possession of sbha and the effective colonization by b. bronchiseptica organisms are closely ... | 1997 | 9035077 |
| effect of bordetella bronchiseptica and serotype d pasteurella multocida bacterin-toxoid on the occurrence of atrophic rhinitis after experimental infection with b. bronchiseptica and toxigenic type ap. multocida. | in efficacy tests, 7 primary specific-pathogen-free piglets vaccinated with the bordetella bronchiseptica and type d pasteurella multocida bacterin-toxoid were challenged with b. bronchiseptica and type a p. multocida. severe or moderate nasal turbinate atrophy was produced in the non-vaccinated pigs, whereas, only one of the 4 pigs in the vaccinated group had slight turbinate atrophy. other immune sera against crude toxin of p. multocida type a or d were cross neutralized. the results of the pr ... | 1997 | 9035080 |
| antimicrobial susceptibility of bordetella bronchiseptica isolates from cats and a comparison of the agar dilution and e-test methods. | one hundred and fifty-two predominantly feline isolates of bordetella bronchiseptica were tested for their susceptibility to seven antimicrobial agents using an agar dilution method. the majority of isolates tested by the agar dilution method were resistant to trimethoprim (mic90 500 micrograms/ml) and ampicillin (mic90 > 32 micrograms/ml) but sensitive to tetracycline, doxycycline and enrofloxacin (mic90 2 micrograms/ml for all three agents). the isolates showed a spectrum of susceptibility to ... | 1997 | 9050171 |
| a porcine model for the evaluation of virulence of bordetella bronchiseptica. | studies of virulence factors of bordetella bronchiseptica require a suitable system. such a system was devised in colostrum-deprived, caesarean-derived pigs, aged 7 d. in two different experiments, pigs (n = 11) were inoculated intranasally with 10(6) colony-forming units of the virulent strain 4609. in the same way, further pigs (n = 11) were inoculated with a strain (b133) of unknown virulence. no significant differences between 4609 and b133 colonization were seen. however, colonization of th ... | 1997 | 9076600 |
| cloning and characterization of an mn-containing superoxide dismutase (soda) of bordetella pertussis. | the fur titration assay (furta) recently developed by i. stojiljkovic and coworkers (j. mol. biol. 236:531-545, 1994) was applied to clone iron-regulated genes of bordetella pertussis. after sequence analysis, one of the clones obtained by this selection procedure was shown to contain an open reading frame with significant sequence similarities to mn-containing superoxide dismutases (soda). the open reading frame was preceded by a fur consensus binding site, which according to primer extension a ... | 1997 | 9079904 |
| cytotoxic necrotizing factor 1 from escherichia coli and dermonecrotic toxin from bordetella bronchiseptica induce p21(rho)-dependent tyrosine phosphorylation of focal adhesion kinase and paxillin in swiss 3t3 cells. | treatment of swiss 3t3 cells with cytotoxic necrotizing factor 1 (cnf1) from escherichia coli and dermonecrotic toxin (dnt) from bordetella bronchiseptica, which directly target and activate p21(rho), stimulated tyrosine phosphorylation of focal adhesion kinase (p125(fak)) and paxillin. tyrosine phosphorylation induced by cnf1 and dnt occurred after a pronounced lag period (2 h), and was blocked by either lysosomotrophic agents or incubation at 22 degrees c. cnf1 and dnt stimulated tyrosine phos ... | 1997 | 9083104 |
| hepatocellular necrosis associated with the subcutaneous injection of an intranasal bordetella bronchiseptica-canine parainfluenza vaccine. | a three-year-old wire fox terrier inadvertently was given an intranasal bordetella bronchiseptica and canine parainfluenza vaccine subcutaneously. the dog subsequently developed both a local inflammatory reaction at the injection site and acute, nonseptic hepatocellular degeneration and necrosis. the patient was treated successfully with intravenous fluids and amikacin. two months after the injection, the serum bile acid concentrations and hepatic histopathology indicated the presence of continu ... | 1997 | 9111722 |
| experimental pneumonia virus of mice infection of guineapigs spontaneously infected with bordetella bronchiseptica. | guineapigs that were intranasally inoculated with pneumonia virus of mice (pvm) seroconverted to pvm by 11 days post-infection. during the course of study (2-60 days post-infection) no gross or histologic lesions were identified within the lungs that could be attributed to pvm infection. mild rhinitis and tracheitis were found in most animals and acute purulent bronchopneumonia in two animals, which may have resulted from spontaneous subclinical bordetella bronchiseptica infection. viral and bac ... | 1997 | 9121108 |
| oligonucleotide probes for bordetella bronchiseptica based on 16s ribosomal rna sequences. | bordetella bronchiseptica 16s ribosomal rna (rrna) gene was cloned and identified. on the basis of information from computer-assisted sequence comparison of the b. bronchiseptica 16s rrna sequences with that of other bacterial species, we constructed b. bronchiseptica-specific oligonucleotide probes complementary to variable regions in the 16s rrna molecule. specificity of these 32p-labeled oligo-nucleotide probes was tested in a rna/dna hybridization with b. bronchiseptica strains and other bac ... | 1994 | 9133055 |
| antigenic polymorphism of the lipopolysaccharides from human and animal isolates of bordetella bronchiseptica. | six monoclonal antibodies (mabs) against lipopolysaccharides (lps) from bordetella pertussis (p1p3, 60.5), b. parapertussis (pp2, pp6, ppb) and b. bronchiseptica (brg1) were used to examine the presence of antigenic determinants of lps on b. bronchiseptica cells. forty-eight clinical isolates of this gram-negative bacterium (4 canine, 3 equine, 6 porcine, 4 rabbit and 31 human) were examined. significant cross-reactivities with the heterologous anti-pertussis and anti-parapertussis mabs were obs ... | 1997 | 9141706 |
| [infection by bordetella bronchiseptica in patients with aids]. | 1997 | 9147515 | |
| temperature dependent expression of an acid phosphatase by bordetella bronchiseptica: role in intracellular survival. | bordetella bronchiseptica has the ability to invade and survive intracellularly. this potential to survive for extended periods within eukaryotic cells might play an important role in the pathogenesis of the infections caused by this microorganism. the bacterial factors involved in this process, however, have not yet been determined. in this study we have identified an acid phosphatase produced by b. bronchiseptica, but not by other bordetella spp. the expression of this enzyme was demonstrated ... | 1997 | 9160295 |
| molecular characterization of two bordetella bronchiseptica strains isolated from children with coughs. | during a surveillance program associated with the italian clinical trial for the evaluation of new acellular pertussis vaccines, two bacterial isolates were obtained in cultures of samples from immunocompetent infants who had episodes of cough. both clinical isolates were identified as bordetella bronchiseptica by biochemical criteria, although both strains agglutinated with antisera specific for bordetella parapertussis, suggesting that the strains exhibited some characteristics of both b. bron ... | 1997 | 9163480 |
| structural characterization of the lipids a of three bordetella bronchiseptica strains: variability of fatty acid substitution. | the structures of lipids a isolated from the lipopolysaccharides (lpss; endotoxins) of three different pathogenic bordetella bronchiseptica strains were investigated by chemical composition and methylation analysis, gas chromatography-mass spectrometry, nuclear magnetic resonance, and plasma desorption mass spectrometry (pdms). the analyses revealed that the lpss contain the classical lipid a bisphosphorylated beta-(1-->6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide ... | 1997 | 9171426 |
| a mutation in the bordetella bronchiseptica bvgs gene results in reduced virulence and increased resistance to starvation, and identifies a new class of bvg-regulated antigens. | the bordetella bvgas signal-transduction system has traditionally been viewed as mediating a transition between two distinct phenotypic phases: the bvg+ phase, characterized by the expression of adhesins and toxins, and the bvg-phase, characterized by motility in bordetella bronchiseptica and by the expression of vrg loci in bordetella pertussis. in b. bronchiseptica, the bvg+ phase is necessary and sufficient for respiratory tract colonization whereas the bvg phase is required for growth under ... | 1997 | 9194696 |
| use of ribotyping to distinguish bordetella bronchiseptica isolates. | a total of 113 bordetella bronchiseptica strains, isolated from 11 different host species worldwide, were characterized by ribotyping with restriction enzyme pvuii. sixteen distinct ribotypes were identified, and each ribotype contained five to seven restriction fragments ranging in size from 1.8 to 5.6 kb. approximately 88% of the swine isoaltes were identified as ribotype 3 strains. isolates from dogs also displayed little variation; 74.1% were found to be ribotype 4 strains. strains obtained ... | 1997 | 9226899 |
| flagellin, a major protein present in sds-page profiles of sarkosyl-omp-enriched fractions from bordetella bronchiseptica bvg- or modulated bvg+ strains. | the bvg or vir locus positively regulates the expression of many bordetella virulence-associated determinants (encoded by vag genes), including cell envelope proteins, in response to environmental stimuli. on the other hand, several genes named vrg genes are negatively controlled by the bvg regulon (knapp and mekalanos, 1988). flagellin is encoded by a vrg gene, which is expressed when the principal virulence factors are eliminated during antigenic modulation or in phase variants (akerley et al. ... | 1997 | 9228683 |
| phase variation affects long-term survival of bordetella bronchiseptica in professional phagocytes. | several bordetella bronchiseptica isolates were investigated for intracellular survival in macrophages. a significant number of viable bacteria of all strains could be recovered even after 96 h postinfection. in all cases bvg mutants of the b. bronchiseptica strains showed a significant survival advantage over the respective wild-type strains. the bacteria were already located in phagolysosomes early after uptake. neither opsonization of the bacteria nor activation of the macrophages with gamma ... | 1997 | 9234815 |
| crystal violet staining of bordetella bronchiseptica colonies for differentiation of phase-i strains from variant strains in degraded phases. | after 2 days of growth on brain heart infusion agar (bhia) at 38 degrees c, phase-i colonies and degraded-phase colonies of bordetella bronchiseptica could be differentiated by their ability to take up crystal violet (cv). phase-i colonies in x mode, but not colonies in degraded phases (phases ii, iii, and rough) bound cv. phenotypically-altered c-mode colonies (grown at 32 degrees c or lower temperatures) also lacked this ability. cv staining offers an easy method for the recognition of differe ... | 1997 | 9243005 |
| analysis of the alcabc operon encoding alcaligin biosynthesis enzymes in bordetella bronchiseptica. | we previously cloned a b. bronchiseptica (bb) genomic dna fragment that complements a bb alcaligin biosynthesis mutant, and reported the identification of a gene, alca, with predicted protein sequence similarity to siderophore biosynthesis enzymes from other organisms. in the present study we show that further nt sequencing of this region revealed two open reading frames (orfs) 3' to alca that encode putative proteins alcb and alcc, with significant sequence similarity to the aerobactin biosynth ... | 1997 | 9266668 |
| cleavage of influenza a virus h1 hemagglutinin by swine respiratory bacterial proteases. | cleavage of influenza a virus hemagglutinin (ha) is required for expression of fusion activity and virus entry into cells. extracellular proteases are responsible for the proteolytic cleavage activation of avirulent avian and mammalian influenza viruses and contribute to pathogenicity and tissue tropism. the relative contributions of host and microbial proteases to cleavage activation in natural infection remain to be established. we examined 23 respiratory bacterial pathogens and 150 aerobic ba ... | 1997 | 9311838 |
| pertactin antigens extracted from bordetella pertussis and bordetella bronchiseptica differ in the isoelectric point. | pertactin, which is a membrane-associated antigen of bordetella pertussis and which is present in many acellular vaccines against whooping cough, has been reported to be similar to the homologous protein in bordetella bronchiseptica. by running parallel experiments using proteins derived from the two species, we show that the isoelectric point of pertactin from b. pertussis is lower than reported and clearly distinguishable from the homologous protein of b. bronchiseptica. | 1997 | 9325433 |
| bordetella bronchiseptica dermonecrotizing toxin induces reorganization of actin stress fibers through deamidation of gln-63 of the gtp-binding protein rho. | bordetella dermonecrotizing toxin causes assembly of actin stress fibers and focal adhesions in some cultured cells and induces mobility shifts of the small gtp-binding protein rho on electrophoresis. we attempted to clarify the molecular basis of the toxin action on rho. analysis of the amino acid sequence of toxin-treated rhoa revealed the deamidation of gln-63 to glu. the substitution of glu for gln-63 of rhoa by site-directed mutagenesis caused a mobility shift on electrophoresis, which was ... | 1997 | 9326660 |
| atypical disease after bordetella pertussis respiratory infection of mice with targeted disruptions of interferon-gamma receptor or immunoglobulin mu chain genes. | using a murine respiratory challenge model we have previously demonstrated a role for th1 cells in natural immunity against bordetella pertussis, but could not rule out a role for antibody. here we have demonstrated that b. pertussis respiratory infection of mice with targeted disruptions of the genes for the ifn-gamma receptor resulted in an atypical disseminated disease which was lethal in a proportion of animals, and was characterized by pyogranulomatous inflammation and postnecrotic scarring ... | 1997 | 9382883 |
| a highly adherent phenotype associated with virulent bvg+-phase swine isolates of bordetella bronchiseptica grown under modulating conditions. | the ability of bvg(-)-phase and bvg(+)-phase bordetella bronchiseptica swine isolates, grown under modulating or nonmodulating conditions, to adhere to swine ciliated nasal epithelial cells was determined. when virulent strains were cultivated at 37 degrees c in the bvg+ phase, numerous adherent bacteria (approximately eight per cell, depending on the strain used) were observed. however, when such strains were grown under modulating conditions (23 degrees c), a significant increase in the level ... | 1997 | 9393829 |
| bordetella bronchiseptica expresses the fimbrial structural subunit gene fima. | the differential host species specificities of bordetella pertussis, b. parapertussis, and b. bronchiseptica might be explained by polymorphisms in adherence factor genes. we have found that b. parapertussis and b. bronchiseptica, unlike b. pertussis, contain a full-length gene for the fimbrial subunit fima. b. bronchiseptica expresses fima in a bvgas-dependent fashion. | 1997 | 9401052 |
| [bronchoalveolar lavage on pig breeding farms]. | in 182 pigs lung lavage was performed using a endotracheal tube and a catheter. the collected bronchoalveolar lavage fluid (balf) was examined microbiologically. with decreasing numbers alpha-hämolytic streptococci, bordetella bronchiseptica, haemophilus parasuis, pasteurella multocida were cultured. actinobacillus pleuropneumoniae was isolated from 3 balfs. in one farm piglets were lavaged routinely for monitoring of the lung health status. | 1997 | 9410726 |
| evolution of a simian immunodeficiency virus pathogen. | analysis of disease induction by simian immunodeficiency viruses (siv) in macaques was initially hampered by a lack of molecularly defined pathogenic strains. the first molecularly cloned siv strains inoculated into macaques, sivmacbk28 and sivmacbk44 (hereafter designated bk28 and bk44, respectively), were cases in point, since they failed to induce disease within 1 year postinoculation in any inoculated animal. here we report the natural history of infection with bk28 and bk44 in inoculated rh ... | 1998 | 9420239 |
| identification and cloning of waaf (rfaf) from bordetella pertussis and use to generate mutants of bordetella spp. with deep rough lipopolysaccharide. | a dna locus from bordetella pertussis capable of reconstituting lipopolysaccharide (lps) o-antigen biosynthesis in salmonella typhimurium sl3789 (rfaf511) has been isolated, by using selection with the antibiotic novobiocin. dna within the locus encodes a protein with amino acid sequence similarity to heptosyltransferase ii, encoded by waaf (previously rfaf) in other gram-negative bacteria. mutation of this gene in b. pertussis, bordetella parapertussis, and bordetella bronchiseptica by allelic ... | 1998 | 9422589 |
| clinical evaluation of a new pcr assay for detection of coxiella burnetii in human serum samples. | a nested pcr method was developed for the detection of coxiella burnetii in human serum samples. two pairs of oligonucleotide primers were designed to amplify a 438-bp fragment of the com1 gene encoding a 27-kda outer membrane protein of c. burnetii. the primers amplified the predicted fragments of 21 various strains of c. burnetii but did not react with dna samples from other microorganisms. the 438-bp amplification products could be digested with restriction enzymes sspi and sali. the utility ... | 1998 | 9431924 |
| use of an enrichment broth cultivation-pcr combination assay for rapid diagnosis of swine erysipelas. | we have previously described the creation by tn916 mutagenesis of avirulent transposition mutants from a highly virulent strain of erysipelothrix rhusiopathiae, the causative agent of swine erysipelas. in this study, we cloned a 2.2-kb dna fragment which flanked the tn916 insertion in an avirulent mutant (strain 33h6) and evaluated the possibility that this region could be used for the specific detection of e. rhusiopathiae. according to the sequences of this region, oligonucleotide primers were ... | 1998 | 9431926 |
| differential regulation of multiple flagellins in vibrio cholerae. | vibrio cholerae, the causative agent of the human diarrheal disease cholera, is a motile bacterium with a single polar flagellum. motility has been implicated as a virulence determinant in some animal models of cholera, but the relationship between motility and virulence has not yet been clearly defined. we have begun to define the regulatory circuitry controlling motility. we have identified five v. cholerae flagellin genes, arranged in two chromosomal loci, flaac and flaedb; all five genes hav ... | 1998 | 9440520 |
| role of bordetella bronchiseptica in infectious tracheobronchitis in dogs. | 1998 | 9448823 | |
| polymorphism in the bordetella pertussis virulence factors p.69/pertactin and pertussis toxin in the netherlands: temporal trends and evidence for vaccine-driven evolution. | the bordetella pertussis proteins p.69 (also designated pertactin) and pertussis toxin are important virulence factors and have been shown to confer protective immunity in animals and humans. both proteins are used in the new generation of acellular pertussis vaccines (acvs), and it is therefore important to study the degree of antigenic variation in these proteins. sequence analysis of the genes for p.69 and the pertussis toxin s1 subunit, using strains collected from dutch patients in the peri ... | 1998 | 9453625 |
| microbiological and clinical aspects of infection associated with stenotrophomonas maltophilia. | the gram-negative bacterium stenotrophomonas maltophilia is increasingly recognized as an important cause of nosocomial infection. infection occurs principally, but not exclusively, in debilitated and immunosuppressed individuals. management of s. maltophilia-associated infection is problematic because many strains of the bacterium manifest resistance to multiple antibiotics. these difficulties are compounded by methodological problems in in vitro susceptibility testing for which there are, as y ... | 1998 | 9457429 |
| characterization of antibiotic resistance plasmids from bordetella bronchiseptica. | of 52 antibiotic-resistant bordetella bronchiseptica isolates from cats, ten carried plasmids. only two of these plasmids, plv1400 and plv1401, were self-transmissible to escherichia coli k12; both plasmids encoded resistance to ampicillin, tetracycline, sulphonamides, streptomycin and mercuric chloride, and were of incompatibility group p (incp). transferable tetracycline resistance has not been reported in b. bronchiseptica previously. the plasmids were identical in size (c.51 kb), restriction ... | 1997 | 9462432 |
| identification of bacterial isolates obtained from intestinal contents associated with 12,000-year-old mastodon remains. | mastodon (mammut americanum) remains unearthed during excavation of ancient sediments usually consist only of skeletal material, due to postmortem decomposition of soft tissues by microorganisms. two recent excavations of skeletal remains in anoxic sediments in ohio and michigan, however, have uncovered organic masses which appear to be remnants of the small and large intestines, respectively. macrobotanical examinations of the composition of these masses revealed assemblages of plant material r ... | 1998 | 9464403 |
| improved diagnostic pcr assay for actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein. | the gene (omla) coding for an outer membrane protein of actinobacillus pleuropneumoniae serotypes 1 and 5 has been described earlier and has formed the basis for development of a specific pcr assay. the corresponding regions of all 12 a. pleuropneumoniae reference strains of biovar 1 were sequenced. alignment of the sequences revealed conserved terminal and variable middle regions, which divided the reference strains into four distinct groups. primers were selected from the conserved 5' and 3' t ... | 1998 | 9466755 |
| transcriptional analysis of the bordetella alcaligin siderophore biosynthesis operon. | the alc gene cluster of bordetella pertussis includes three genes, alca, alcb, and alcc, which are involved in alcaligin siderophore biosynthesis in response to iron starvation. the production of alca, alcb, and alcc in bordetella cells and the transcriptional organization of alca, alcb, and alcc were investigated by using a set of three alc'-'lacz gene fusion constructs that were contiguous with the known promoter upstream of alca and extended to fusion junctions within each alc cistron. all th ... | 1998 | 9473039 |
| identification and characterization of alcr, a gene encoding an arac-like regulator of alcaligin siderophore biosynthesis and transport in bordetella pertussis and bordetella bronchiseptica. | a bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. the mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. in addition to the apparent inability to acquire iron from the siderophore, the mutant failed to produce alcaligin as well as two known iron-regulated proteins, one of which is the alcc alcaligin biosynthesis protein. a 1.6-kb kpni-psti bordetella pert ... | 1998 | 9473040 |