Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| protection against experimental bordetella bronchiseptica infection in mice by active immunization with killed vaccine. | mice immunized with a killed vaccine of phase i bordetella bronchiseptica were challenged with various numbers of virulent b. bronchiseptica by intraperitoneal, intracerebral, or intranasal routes. the course of infection was compared among these routes, and the protective effect of vaccination was quantitatively analyzed. in ddn mice infected intraperitoneally with 1.8 x 10(8) cells (ca. 80 times the 50% lethal dose [ld50]) the organisms rapidly increased in the intraperitoneal fluid, spleen, a ... | 1983 | 6874070 |
| virulence of pasteurella multocida in atrophic rhinitis of gnotobiotic pigs infected with bordetella bronchiseptica. | bordetella bronchiseptica and pasteurella multocida have been implicated in the aetiology of atrophic rhinitis of pigs but the precise cause and pathogenesis of field outbreaks have still to be clarified. the virulence of 11 strains of p multocida was investigated by intraperitoneal injection of culture filtrates in balb/c mice, or by infection of gnotobiotic piglets given b bronchiseptica five days previously. three of four type d strains of p multocida were lethal for mice and caused severe tu ... | 1983 | 6878879 |
| an outbreak of bordetella bronchiseptica respiratory disease in foals. | 1981 | 6908767 | |
| bordetella bronchiseptica associated with pulmonary disease in mountain voles (microtus montanus). | bordetella bronchiseptica was isolated from the lungs of all of six mountain voles (microtus montanus) found dead or dying of pulmonary infection near the bear river research station in northern utah in january, 1973. the possibility of concomitant viral or mycoplasmal infection was not ruled out. | 1980 | 6990019 |
| high prevalence of strains of bordetella bronchiseptica resistant to potentiated sulphonamide in english pig herds in 1978-79. | 1980 | 7001722 | |
| biology of bordetella bronchiseptica. | 1980 | 7010115 | |
| [production of a high-titer agglutinating bordetella bronchiseptica serum]. | for a serological identification of bordetella bronchiseptica, eliminated from swine ill with atrophic rhinitis, was obtained an agglutinated antiserum. for the purpose rabbits were innoculated 1. w. 2.5-3 kg) and living cultures were used after intravenous injection. for the obtention of a bacterial suspension müller-hinton's medium was used. three series of antiserum were produced against bordetella bronchiseptica. the first series was obtained after straining the bacterial strain into the med ... | 1980 | 7010770 |
| use of the api 20e system to identify non-enterobacteriaceae from veterinary medical sources. | the capability of the api 20e system to identify gram-negative nonfermenters and nonenteric fermenters was evaluated for 272 isolates from veterinary sources. two different methods were used for interpreting the carbohydrate fermentation reactions on the strip. in method i, weakly fermented (yellow-green) carbohydrates were considered positive for all oxidase-positive organisms, and in method ii, yellow-green carbohydrates were considered positive for all organisms requiring incubation for 48 ho ... | 1981 | 7023301 |
| [bordetella bronchiseptica infection in a spf pig herd. contribution to the aetiology of atrophic rhinitis (a. r.) (author's transl)]. | 1982 | 7044749 | |
| [use of various methods of sampling and various culture media in the isolation of bordetella bronchiseptica from swine in the field (author's transl)]. | during eleven visits to nine pig-breeding farms and multiplying herds, 190 piglets were sampled by three different methods. the nares were separately sampled and both nares were sampled with a single swab. three media were used to discover which method and which medium produced the highest isolation-score for bordetella bronchiseptica (bb). the cdi-mac conkey (mcc-cdi) plate was used as a reference medium and was compared with the gezondheidsdienst mac conkey (mcc-gd) and the new bordetella plat ... | 1982 | 7048626 |
| symposium on infective endocarditis. iii. endocarditis due to gram-negative bacteria. report of 56 cases. | at the mayo clinic, 56 patients with infective endocarditis caused by gram-negative bacteria were seen from 1958 through 1979, 35 of whom were seen from 1970 through 1979. the patients were categorized into two divisions: those with medical, naturally acquired valve infections (40 [71%]) and those with infective endocarditis after cardiac operation (16 [29%]). the overall cure rate was 82% (46 of 56 patients); 35 of 40 patients (88%) were cured in the medical group, and 11 of 16 patients (69%) w ... | 1982 | 7062778 |
| long-term effect of bordetella bronchiseptica infection in neonatal pigs. | a total of 62 gnotobiotic pigs were inoculated with bordetella bronchiseptica at 7 days of age. there were 25 noninoculated controls. thirteen of the inoculated pigs were killed and 6 pigs which died were examined between 3 and 5 weeks after inoculations were done (group a). of these 19 pigs, 16 had severe pneumonia and b bronchiseptica was isolated from the lungs of 17. the turbinates in most of the pigs appeared to have some atrophy. four other pigs died of pneumonia after they were transferre ... | 1982 | 7073084 |
| some characteristics of a urease-negative, temperature- sensitive strain of bordetella bronchiseptica as a live, attenuated vaccine. | strain ts-s34 of bordetella bronchiseptica was treated jointly with two mutagens, nitrosoguanidine and uv irradiation, and a urease-negative (u-), temperature-sensitive (ts) mutant strain indistinguishable from alcaligenes faecalis in its biochemical characteristics was isolated. however, the mutant isolated was a phase iii organism. by repeating selection of smaller, hemolytic colonies observed among phase iii colonies after prolonged incubation, a phase i organism, strain ts-s34.u-, was isolat ... | 1982 | 7076293 |
| abdominal mesothelioma in an aged guinea pig (cavia porcellus). | an adult, male guinea pig died of bordetella bronchiseptica pneumonia during quarantine and was necropsied, numerous, firm, pale nodules were found attached to the omentum, the parietal peritoneum, and the serosal surface of the liver and intestinal tract. gross, light, and electron microscopic examination revealed the tumors to be mesotheliomas. to our knowledge, mesotheliomas have not been reported in this species. | 1982 | 7078085 |
| immunogenicity of killed bordetella bronchiseptica vaccines in the mouse. | two intramuscular injections (two weeks apart of graded doses of killed strains of bordetella bronchiseptica from the pig (oln 14 or lbf 1) or the dog (d1) had produced in mice circulating agglutinins ranging in mean titre (log2) per group from about 3.3 to 10.2 two weeks later. these levels depended partly on vaccinal strains and dose, and partly on the strain used as agglutinogen. other such mice were challenged intraperitoneally with about 50 ld50 (approximately or equal to 10(7.4) viable bac ... | 1982 | 7079606 |
| glucose-nonfermenting gram-negative bacilli associated with clinical veterinary specimens. | glucose-nonfermenting gram-negative bacilli (nfb) have been recognized recently as opportunistic pathogens of humans. with few exceptions, strains of nfb have not been considered important enough to be identified when isolated from animals. in this study, all nfb isolated during a 1-year period in a clinical veterinary microbiology laboratory were identified to determine their prevalence. of the 347 strains of nfb obtained, the most common species were pseudomonas aeruginosa, acinetobacter calco ... | 1982 | 7107835 |
| a study of chronic pneumonia in a guineapig colony with enzootic bordetella bronchiseptica infection. | three types of lesion were present in the lungs of guineapigs with chronic pneumonia - pulmonary interstitial fibrosis, perivascular lymphoid hyperplasia and foreign body granulomas. bordetella bronchiseptica was present in the nasopharynx, trachea and lungs of a high proportion of animals from 4 weeks old, and antibodies to this organism were also found. animals removed at 4 days old and reared in isolation from the main colony and free from b. bronchiseptica developed similar lung lesions, so ... | 1982 | 7109535 |
| immunisation of pigs against experimental infection with bordetella bronchiseptica. | during pregnancy seven minimum-disease sows (group a) were infected intranasally with bordetella bronchiseptica, fed with the killed bacterium periodically and inoculated parenterally with a dead vaccine eight, six and two weeks before parturition. groups b and c, isolated from a until farrowing, contained respectively six sows given the vaccine parenterally and eight control sows. at parturition, group a had much higher average agglutinin titres in the serum and colostrum than b or c. group a s ... | 1982 | 7112861 |
| ultrastructural changes in the nasal turbinate bones of pigs in early infection with bordetella bronchiseptica. | the ultrastructural changes in the turbinates of young pigs killed four, seven, 14 and 28 days after intranasal inoculation (dpi) with bordetella bronchiseptica were restricted to osteoblasts. in a few cells irregularities and evagination of the nuclear membrane were associated with enlargement of the golgi apparatus at four dpi. by seven dpi many osteoblasts had these changes and also a dilatation of the endoplasmic reticulum while mitochondria retained normal definition: an amorphous degenerat ... | 1982 | 7134647 |
| experimental infection of bordetella bronchiseptica to rabbits. | infectivity and pathogenicity of bordetella bronchiseptica to rabbits were investigated by intranasal inoculation of the organism to suckling and young animals. results are summarized as follows. 1. by inoculation of 5 x 10(4) and 10(6) organisms, the infection developed in 60% and 100% of young rabbits, respectively. 2. in young rabbits, the growth of the organism was evident in the trachea within 5 days after inoculation and in the lung on the 10th day. the organism began to disappear from the ... | 1982 | 7140824 |
| virulence of bordetella bronchiseptica from pigs with or without atrophic rhinitis. | the virulence of 17 isolates of bordetella bronchiseptica from 13 pig herds was compared by intranasal infection of gnotobiotic piglets and ld50 tests on mice. of 59 piglets given 8.1-10-5 log 10 colony-forming units (cfu) of isolates from two herds with atrophic rhinitis (ar isolates) or isolates from six unaffected herds (non-ar isolates), 16 died of acute pneumonia; the survivors developed non-progressive turbinate hypoplasia and chronic pneumonia. infection of 11 piglets with c. 3.0 log to c ... | 1982 | 7143418 |
| nasal turbinate atrophy in young mice inoculated with bordetella bronchiseptica of pig origin. | development of a model in experimental small animals will facilitate research on the mechanisms of nasal turbinate atrophy of swine. atrophy of the nasal turbinate with bordetella bronchiseptica phase i organisms of pig origin was induced by intranasal inoculation in 5-day-old or younger mice. inoculations of phase i organisms into 10-day-old or older mice and of avirulent phase iii organisms into 5-day-old or younger mice did not induce lesions of the disease, however. the lesions appeared in 4 ... | 1982 | 7149389 |
| occurrence and characterization of plasmids in field isolates of bordetella bronchiseptica. | twenty-seven isolates of bordetella bronchiseptica were examined for the presence of plasmid dna. eleven of the isolates contained plasmids. an attempt was made to correlate the presence of plasmids with resistance to a number of antibiotics or heavy metals or with production of bacteriocins. eight of the isolates contained a plasmid of approximately 35 megadaltons molecular weight; these isolates were all resistant to 1 mm mercuric chloride. there were no other correlations between plasmid cont ... | 1982 | 7149391 |
| [differentiation of bordetella bronchiseptica strains. 1. detection of heat-labile exotoxins and hemagglutinins]. | 1982 | 7165453 | |
| [differentiation of bordetella bronchiseptica strains. 2. determination of protective properties of selected strains in a cross-protection test]. | 1982 | 7165454 | |
| atrophic rhinitis: appraisal of infection pressure on gnotobiotic piglets infected with bordetella bronchiseptica. | with the use of 16 gnotobiotic piglets inoculated at day 4, 5 and 6 of life with defference concentrations (3.10(4) to 3.10(9) colony forming units of bordetella bronchiseptica per ml), it was possible to establish a minimal infective dose (3.10(5) cfu/ml). with a lower dose, it was not possible to induce any of the typical gross lesions of atrophic rhinitis. the authors discuss some factors which can modify the infection pressure in the field. | 1982 | 7172101 |
| effect on the incidence of atrophic rhinitis of vaccination of sows with a vaccine containing pasteurella multocida toxin. | combined experimental infections with bordetella bronchiseptica and either a toxigenic or a non-toxic strain of p. multocida were carried out in newborn specific pathogen free piglets born to 25 sows. pigs inoculated with b. bronchiseptica and toxin-producing p. multocida developed severe progressive atrophic rhinitis corresponding to the natural disease. the effect of vaccination of sows with a toxin-containing p. multocida vaccine on the incidence of nasal lesions in the offspring was studied. | 1982 | 7177813 |
| an elisa test to detect antibody to bordetella bronchiseptica. | an enzyme-linked immunosorbent assay (elisa) was developed to measure antibody to bordetella bronchiseptica in dogs. the elisa test was more rapid and sensitive and required 50 to 150 times less antigen than the amount of antigen required for the conventional tube agglutination test. a survey of 50 canine serum samples using elisa suggested that 8% of all sera had titers greater than 1:64, 56% had titers of 1:8 to 1:64, and 36% had titers of less than 1:8. the mean titer of survey sera was 1:46 ... | 1982 | 7179721 |
| [epizootiological studies of atrophic rhinitis in swine. ii. biological properties of bordetella bronchiseptica strains isolated from swine]. | 1982 | 7180250 | |
| [effect of cannabinoids on the immune response of rabbits to bordetella bronchiseptica antigen]. | 1982 | 7187706 | |
| [variability of the dose effect in the radio-biologic experimentation on animals. second communication: influence of breed, sex, age as well as of infections on death rates and ld 50 values (author's transl)]. | as already demonstrated for wistar rats, the radioresistance of siv-50 male and female rats increases continually during their first months of life. furthermore, males show a higher radioresistance than females during this period. the authors show with the same animals that a very radiosensitive rat strain can become a highly radioresistant strain after 2 1/2 years. after whole-body irradiation with 800--900 r, the increase of resistance of 85 days old males was between 62% and 68%. as a cause o ... | 1981 | 7222131 |
| selective media for pasteurella multocida and bordetella bronchiseptica. | easily prepared and inexpensive culture medium for the isolation of pasteurella multocida and bordetella bronchiseptica from the nares of laboratory rabbits was prepared by incorporating 2 micrograms/ml clindamycin into standard blood agar. using this medium, the time and materials required to identify and isolate pasteurella multocida was substantially reduced with only a slight loss in sensitivity. the combination of 2 micrograms/ml clindamycin and 4 micrograms/ml neomycin was effective in iso ... | 1981 | 7253549 |
| treatment of experimental bordetella bronchiseptica infection in young pigs with potentiated sulphonamide in the drinking water. | bordetella bronchiseptica was eliminated from the nasal cavity of experimentally infected piglets after about three weeks by trimethoprim and sulphadiazine (potentiated sulphonamide) in the drinking water in two experiments (at levels of 13.3 and 66.7 micrograms per ml, respectively). the rhinitis and turbinate damage associated with the infection was significantly less when the animals were examined at seven weeks of age but daily weight gain was not improved to a significant extent compared wi ... | 1981 | 7269193 |
| intranasal vaccination of dogs with liver avirulent bordetella bronchiseptica: correlation of serum agglutination titer and the formation of secretory iga with protection against experimentally induced infectious tracheobronchitis. | dogs inoculated intranasally with a live avirulent bordetella bronchiseptica vaccine were monitored for the development of resistance to experimentally induced infectious tracheobronchitis (canine cough). dogs were challenge exposed with a virulent strains of b bronchiseptica at various times after they were vaccinated. clinical protection was detectable as early as 48 hours. at postvaccination days 4, 5, and 14, 56%, 83%, and 95% protection was observed. humoral immunoglobulin (ig) titers range ... | 1981 | 7271029 |
| bordetella bronchiseptica peritonitis associated with continuous ambulatory peritoneal dialysis. | bordetella bronchiseptica has rarely been reported as an opportunistic pathogen in humans. this is the first documented report of peritonitis due to b. bronchiseptica. the organism was seen on a gram strain of peritoneal fluid and was isolated from a culture of the fluid. it was thought that the source of infection was related to the close contact of the patient with his dog. the patient was successfully treated with chloramphenicol administered intraperitoneally and intravenously. | 1981 | 7276151 |
| effects of atmospheric ammonia on young pigs experimentally infected with bordetella bronchiseptica. | effects of atmospheric ammonia on performance and respiratory tract health of young pigs experimentally infected with bordetella bronchiseptica were studied. treatments were: (1) control, (2) bordetella inoculation (approx 10(9) bacteria/naris) alone, (3) bordetella inoculation plus exposure to atmospheric ammonia at 34.7 mg/m3 (50 ppm), and (4) bordetella inoculation plus exposure to atmospheric ammonia at 69.4 mg/m3 (100 ppm). pigs weighted 8.01 kg (av) at start of treatment. body weight and f ... | 1981 | 7283246 |
| an improved system for studying the effect of bordetella bronchiseptica on the ciliary activity of canine tracheal epithelial cells. | ciliated epithelial cell outgrowths from canine tracheal explants were used to study the interaction between bordetella bronchiseptica and canine respiratory cilia. a sensitive system for quantitating the ciliary beat frequencies of an individual cell was used to study the effects of b. bronchiseptica on ciliary function. phase i and intermediate-phase b. bronchiseptica isolates produced significant reductions (greater than or equal to 50%) in ciliary beat frequencies within 5 min and nearly com ... | 1981 | 7288215 |
| quantitative observations on bordetella bronchiseptica infection in atrophic rhinitis of pigs. | clinical atrophic rhinitis in seven pig herds could not be associated with the infection rate or higher numbers of b bronchiseptica in nasal swabs when compared with unaffected herds. b bronchiseptica isolates from herds with atrophic rhinitis and receiving sulphonamide medication were resistant to sulphonamides in vitro and there was a beneficial clinical response after changing to oxytetracycline medication. in an unaffected herd three piglets naturally infected with b bronchiseptica but posse ... | 1981 | 7292916 |
| the aetiological significance of bordetella bronchiseptica and pasteurella multocida in atrophic rhinitis of swine. | experimental infection with either pure culture of bordetella bronchiseptica or b. bronchiseptica and a toxin-producing strain of pasteurella multocida was studied in newborn piglets from 26 sows. pigs inoculated with b. bronchiseptica alone got mild lesions, whereas pigs inoculated with both organisms showed clinical and pathological signs of atrophic rhinitis, corresponding to the progressive natural diseases. the effect of vaccinating sows against b bronchiseptica during pregnancy was studied ... | 1981 | 7335484 |
| bordetella bronchiseptica infection in stage iv hodgkin's disease. | 1981 | 7339608 | |
| non-conjugative r plasmid with five drug resistance from bordetella bronchiseptica of pig origin. | 1981 | 7343701 | |
| scanning electronmicroscopic studies of bordetella bronchiseptica on the rabbit tracheal mucosa. | rabbit tracheal mucous membrane cultivated on solid agar medium was infected with either bordetella bronchiseptica or staphylococcus aureus. scanning electron microscopy revealed that large numbers of b. bronchiseptica cells became entangled with the cilia of the tracheal epithelial cells. in contrast, s. aureus cells showed no such intimate interaction with the cilia. | 1980 | 7359573 |
| [use of an oxytetracycline nasal spray in the treatment and prevention of atrophic rhinitis in piglets (author's transl)]. | the piglets of two multiplier herds (m and b) showing clinically apparent atrophic rhinitis (ar) were treated by the nasal-spray method. a solution of oxytetracycline hydrochloride (otc, 50 mg/ml.) was used as a spray fluid. the course of the disease in the herds was followed by studying the development of foreshortening of the upper jaws in the heads. brachygnathia superior (bs), from the eighth to tenth week of life. efforts were made to gain an impression of the effects of treatment on the fr ... | 1980 | 7404566 |
| [some aspects of the study of atrophic rhinitis in pigs (author's transl)]. | atrophic rhinitis is the most dreaded nasal inflammatory lesion described in pigs. experimental studies showed that this form of rhinitis may be induced by inoculation of the bacterial species bordetella bronchiseptica (bb) and/or pasteurella multocida (pm). it is apparent from studies on ar in the central veterinary institute during the past few years, that these species of bacterium may be classified into ar-pathogenic and ar-non-pathogenic micro-organisms. the significance of these findings i ... | 1980 | 7423466 |
| medicated early weaning to obtain pigs free from pathogens endemic in the herd of origin. | a field trial was conducted to assess the value of medicated early weaning for obtaining pigs free from some of the pathogens endemic in their herd of origin. the trial comprised 51 sows from a closed herd, which were farrowed in an isolated farrowing house in seven separate groups. the sows in each group were bred at the same time and induced to farrow on the same day. their thriftiest piglets were weaned at five days of age and moved to an isolated early-weaning unit. at about six weeks of age ... | 1980 | 7445326 |
| the prevalence of brodetella bronchiseptica and turbinate (conchal) atrophy in english pig herds in 1978-79. | examination of pigs (eight to 10 per farm) slaughtered between december 1978 and march 1979 at abattoirs in southern england indicated that infection with bordetella bronchiseptica was widespread. the organism was recovered from the nasal cavity of 424 out of 844 (50 per cent) of such pigs and these were distributed among 79 out of 86 (91 per cent) of the herds submitting animals. the sensitivity to sulphonamide or potentiated sulphonamide of 255 of these strains was determined: in 20 out of 70 ... | 1980 | 7445392 |
| experimental atrophic rhinitis in gnotobiotic pigs. | twenty-nine caesarian derived colostrum deprived germfree pigs were reared in isolators in groups of three to four per isolator. at seven days of age each group was inoculated intranasally with one of four strains of bordetella bronchiseptica (designated b, j, l and 55b), or pseudomonas aeruginosa or a mucoid strain of escherichia coli, all previously isolated from nasal mucus of pigs affected with clinical atrophic rhinitis. another group was inoculated simultaneously with b. bronchiseptica b a ... | 1980 | 7448626 |
| [field trial for control of atrophic rhinitis by vaccination with bordetella bronchiseptica]. | 1980 | 7466336 | |
| vaccination against atrophic rhinitis in swine with a combined bordetella bronchiseptica, pasteurella multocida vaccine. | 1980 | 7467988 | |
| tracheal colonization factor: a bordetella pertussis secreted virulence determinant. | we report here the identification of a virulence-associated factor, tcf, (tracheal colonization factor), produced by strains of bordetella pertussis but not bordetella parapertussis or bordetella bronchiseptica. this protein is encoded by the tcfa gene. when a strain of b. pertussis 18323 lacking this protein is used to infect mice with an aerosol challenge, the number of bacteria isolated from the tracheas is decreased 10-fold when compared with the parent 18323. the derived amino acid sequence ... | 1995 | 7476158 |
| isolation and characterization of pseudomonas pseudomallei flagellin proteins. | flagellin proteins from several different strains of pseudomonas pseudomallei have been isolated and purified to homogeneity by mechanical shearing and differential centrifugation techniques. analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded flagellin monomer protein bands with an estimated m(r) of 43,400. no lipopolysaccharide contamination of the purified protein preparations was detectable by silver staining of flagellin displayed on polyacrylamide gels and by west ... | 1994 | 7513308 |
| monophyletic origin of beta-division proteobacterial endosymbionts and their coevolution with insect trypanosomatid protozoa blastocrithidia culicis and crithidia spp. | some trypanosomatid protozoa (order kinetoplastida) are well known to harbor bacterial endosymbionts. their phylogenetic positions and evolutionary relationships with the hosts were deduced by comparing the rrna gene sequences. earlier, we observed that these symbionts from three crithidia spp. are identical and are closely related to bordetella bronchiseptica. we have now sequenced the genes of another endosymbiont and the host protozoan blastocrithidia culicis. the 16s rrna genes of the blasto ... | 1994 | 7521530 |
| specific and cross-reacting monoclonal antibodies to bordetella parapertussis and bordetella bronchiseptica lipopolysaccharides. | three groups of monoclonal antibodies (mabs) were produced that would be useful for immunochemical typing and diagnosis of infections due to bordetella species, and for the structural analysis of their lipopolysaccharides. pp6, a representative of the first group, recognizes an epitope shared by smooth-type bordetella parapertussis and bordetella bronchiseptica lipopolysaccharides (lps). this epitope is carried by structurally identical polymeric o-chains (poc) present on both lps molecules. pp8 ... | 1994 | 7524956 |
| cloning and sequence of the bordetella bronchiseptica adenylate cyclase-hemolysin-encoding gene: comparison with the bordetella pertussis gene. | the cyaa gene from bordetella bronchiseptica (bb), encoding the adenylate cyclase-hemolysin (ac-hly), has been cloned and its complete nucleotide sequence has been determined. the deduced amino-acid sequence was compared to the ac-hly from b. pertussis (bp) and the main differences were found in the c-terminal repeat region of the molecule. | 1995 | 7557410 |
| human bordetella bronchiseptica infection related to contact with infected animals: persistence of bacteria in host. | within a period of 2 1/2 years, bordetella bronchiseptica was isolated four times from a 79-year-old woman with bronchopneumonia. we have demonstrated by pulsed-field gel electrophoresis that this infection was related to contact with infected rabbits. the initial human b. bronchiseptica isolate had a phenotype characteristic of usual b. bronchiseptica clinical isolates; it produced toxin and adhesins, such as adenylate cyclase-hemolysin, filamentous hemagglutinin, and pertactin, and was able to ... | 1995 | 7559937 |
| [comparative susceptibility of ochrobactrum anthropi, agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes denitrificans subsp. denitrificans, alcaligenes denitrificans subsp. xylosidans and bordetella bronchiseptica against 35 antibiotics including 17 beta-lactams]. | ochrobactrum anthropi, formerly known as "achromobacter sp." or cdc group vd has been isolated from water, hospital environment (antiseptic solutions, dialysis fluids ... ). o. anthropi is a gram negative, motile, strictly aerobic, oxydase positive and non-fermentative bacteria with a strong urease activity. the susceptibility of 13 strains of o. anthropi was determined by agar diffusion method and compared to those of type strains of agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes ... | 1995 | 7567111 |
| bvg repression of alcaligin synthesis in bordetella bronchiseptica is associated with phylogenetic lineage. | recent studies have shown that bordetella bronchiseptica utilizes a siderophore-mediated transport system for acquisition of iron from the host iron-binding proteins lactoferrin and transferrin. we recently identified the b. bronchiseptica siderophore as alcaligin, which is also produced by b. pertussis. alcaligin production by b. bronchiseptica is repressed by exogenous iron, a phenotype of other microbes that produce siderophores. in this study, we report that alcaligin production by b. bronch ... | 1995 | 7592367 |
| bordetella bronchiseptica dermonecrotizing toxin stimulates assembly of actin stress fibers and focal adhesions by modifying the small gtp-binding protein rho. | we studied the biochemical mechanism of morphological changes in cells treated with bordetella dermonecrotizing toxin (dnt). dnt caused the morphological changes of serum-starved mc3t3-e1 cells from flat shapes to reflactile ones. these changes were accompanied by the assembly of actin stress fibers and focal adhesions, which is known to be regulated by the small gtp-binding protein rho. clostridium botulinum c3 exoenzyme, which adp-ribosylates and inactivates rho protein, 'rounded' the cells wi ... | 1995 | 7593285 |
| influence of culture conditions on expression of antigens in bordetella bronchiseptica. | bordetella (b.) bronchiseptica isolates from the respiratory tract of rat and pig in their virulent phase-i and their spontaneously developed avirulent phase-iii were investigated. the strains were cultured on bordet-gengou agar, on nutrition agar with 10% horse blood, and with 5% sheep blood, on yeast agar, on minimal nutrition agar (mm) and simmon's citrate agar with glycerol, starch, and nicotinic acid. antigenic modulation was induced by mgso4 on bordet-gengou agar. the b. bronchiseptica str ... | 1994 | 7597857 |
| identification of a bordetella pertussis regulatory factor required for transcription of the pertussis toxin operon in escherichia coli. | transcription of the pertussis toxin operon (ptx) is positively regulated in bordetella pertussis by the bvgas locus. however, a ptx-lacz transcriptional fusion in escherichia coli cannot be activated by bvgas in trans. this suggests that an additional factor(s) is required for transcription of ptx. a gene encoding a bvg accessory factor (baf) was identified by its ability to activate an e. coli ptx-lacz fusion in the presence of bvgas. the expression of ptx-lacz was decreased by the addition of ... | 1995 | 7601846 |
| localization of the promoter for the ptl genes of bordetella pertussis, which encode proteins essential for secretion of pertussis toxin. | the ptl locus of bordetella pertussis, which encodes proteins necessary for the secretion of pertussis toxin into the extracellular medium, is located directly downstream from the ptx locus, which encodes the structural subunits of the toxin. we have found that the ptx promoter is essential for expression of the ptl genes. a strain of b. pertussis which lacked only the ptx promotor region but which retained all other portions of the ptx-ptl region did not produce ptlf. moreover, insertion of a f ... | 1995 | 7622254 |
| clinical pharmacologic aspects of cefixime in dogs. | the minimal inhibitory concentration (mic) of cefixime, a new third-generation orally administered caphalosporin, was determined for reference and clinical isolates from dogs. the mic of the drug for all but 1 of the 18 enterobacteriaceae isolates tested, 1 pasteurella canis, 1 rhodococcus equi, 1 streptococcus canis, and 1 streptococcus group g isolate, was less than 1.0 microgram/ml. the mic for 9 staphylococcus intermedius isolates ranged from 1.56 to 6.25 micrograms/ml and, for 8 sta aureus ... | 1995 | 7661460 |
| nucleotide sequences of the 23s rrna genes from bordetella pertussis, b.parapertussis, b.bronchiseptica and b.avium, and their implications for phylogenetic analysis. | 1993 | 7688118 | |
| multifactorial analysis of antibiotic sensitivity of bordetella bronchiseptica isolates from guineapigs, rabbits and rats. | the possible relationship between antibiotic sensitivity of b. bronchiseptica isolates and host species was examined. growth inhibition of 11 b. bronchiseptica isolates from guineapigs, 7 from rabbits and 3 from rats by a variety of antibiotics incorporated in paper disks, was tested. to seek possible relationships among the various antibiotic sensitivities, we submitted our datamatrix to principal component analysis and subsequently to cluster analysis. this approach showed the existence of clo ... | 1995 | 7707677 |
| bordetella bronchiseptica infection in patients with aids. | 1995 | 7727654 | |
| non-opsonic attachment of bordetella bronchiseptica mediated by cd11/cd18 and cell surface carbohydrates. | porcine atrophic rhinitis associated with bordetella bronchiseptica is characterized by a severe inflammatory response in the mucosa of the nasal turbinates. initial infiltrates of polymorphonuclear leukocytes (pmn) are followed by accumulations of mononuclear cells. in this report, we have investigated the interaction between porcine pmn and b. bronchiseptica. pmn incubated in pbs with a fluorescently labeled hemagglutinating porcine isolate, but not a non-hemagglutinating variant, had high lev ... | 1994 | 7752879 |
| bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated bordetella bronchiseptica mutants. | we report the isolation and preliminary phenotypic characterization of manganese-resistant bordetella bronchiseptica mutants with respect to deregulation of siderophore and iron-regulated protein expression. the fur gene of bordetella pertussis was cloned by genetic complementation of this deregulated phenotype and confirmed as fur by nucleotide sequence analysis. | 1995 | 7798143 |
| bacteremia caused by a novel bordetella species, "b. hinzii". | bordetella spp. cause respiratory tract diseases in warm-blooded animals. only bordetella bronchiseptica has been reported to cause bacteremia in humans, and this rare infection usually occurs with pneumonia in immunocompromised patients. we describe "bordetella hinzii" bacteremia in an aids patient without a respiratory illness. combining biochemical phenotyping with fatty acid analysis permitted preliminary identification of this previously undescribed pathogen; identity was confirmed by dna-d ... | 1994 | 7814500 |
| interlaboratory comparison of enzyme-linked immunosorbent assay (elisa) and indirect immunofluorescence (iif) for detection of bordetella bronchiseptica antibodies in guinea pigs. | thirty-nine guinea pigs were examined for bordetella bronchiseptica infection by culture and serology, using the enzyme-linked immunosorbent assay (elisa) and the indirect immunofluorescence (iif) test. each serological method detected evidence of b. bronchiseptica infection in more guinea pigs than did culture. iif using an antigen prepared from a mouse isolate of b. bronchiseptica detected fewer infected guinea pigs than when performed with antigens prepared from b. bronchiseptica isolates fro ... | 1994 | 7830373 |
| [sinusitis and bordetella bronchiseptica pneumonia in aids]. | 1994 | 7831233 | |
| identification of a bordetella pertussis bvg-regulated porin-like protein. | bordetella pertussis 18323 produces a bvg-regulated 39.1-kda porin-like protein, ompq. ompq had 61% similarity to the major porin of b. pertussis and contains conserved regions common to both the neisserial and enteric porin families. the results of southern blot analysis indicate that strains of bordetella parapertussis and bordetella bronchiseptica but not bordetella avium contain this gene. | 1995 | 7836316 |
| differentiation of brucella abortus bv. 1, 2, and 4, brucella melitensis, brucella ovis, and brucella suis bv. 1 by pcr. | several pcr assays which identify the genus brucella but do not discriminate among species have been reported. we describe a pcr assay that comprises five oligonucleotide primers which can identify selected biovars of four species of brucella. individual biovars within a species are not differentiated. the assay can identify three biovars (1, 2, and 4) of b. abortus, all three biovars of b. melitensis, biovar 1 of b. suis, and all b. ovis biovars. these biovars include all of the brucella specie ... | 1994 | 7852552 |
| identification of alcaligin as the siderophore produced by bordetella pertussis and b. bronchiseptica. | the siderophores produced by iron-starved bordetella pertussis and b. bronchiseptica were purified and were found to be identical. using mass spectrometry and proton nuclear magnetic resonance, we determined that the siderophore produced by these organisms was identical to alcaligin, a siderophore produced by alcaligenes denitrificans. | 1995 | 7860593 |
| serum c-reactive protein and immune responses in dogs inoculated with bordetella bronchiseptica (phase i cells). | eight beagle dogs were inoculated intrabronchially with 5 x 10(9) live, avirulent cells of bordetella bronchiseptica l-414 strain (phase i cells) (b. bronchiseptica) to investigate the serum levels of their c-reactive protein, the white blood cell counts, the antibody responses to b. bronchiseptica in the sera and tracheal secretions, and the effects of prednisolone given to four of the dogs on c-reactive protein (crp), white blood cells (wbc) and immune responses. in two beagle dogs inoculated ... | 1994 | 7863606 |
| pasteurella multocida produces a protein with homology to the p6 outer membrane protein of haemophilus influenzae. | an antibody specific for a 16-kda outer membrane protein of a rabbit strain of pasteurella multocida was used to probe representatives of all 16 somatic serotypes of p. multocida, as well as the vaccine strains cu and m9, and all were shown to express the protein. the gene encoding this protein was cloned and sequenced and found to have extensive sequence homology with the gene encoding the p6 protein of haemophilus influenzae. the protein in p. multocida has been designated p6-like. the gene en ... | 1995 | 7868272 |
| seroprevalence and isolation rate of bordetella bronchiseptica in cats in the uk. | 1994 | 7871691 | |
| bordetella bronchiseptica pneumonia. | 1994 | 7878573 | |
| bordetella bronchiseptica in an aids patient cross-reacts with legionella antisera. | 1994 | 7883914 | |
| cloning and sequencing of a bordetella pertussis serum resistance locus. | we have characterized a new virulence factor in bordetella pertussis: serum resistance. compared with escherichia coli hb101, wild-type b. pertussis was relatively resistant to classical-pathway, complement-dependent killing by normal human serum. however, a mutant of b. pertussis (bpm2041) which is less virulent in mice and which has tn5 lac inserted in a previously uncharacterized bvg-regulated gene was found to be at least 10-fold more susceptible to serum killing than the wild type. we have ... | 1994 | 7927748 |
| invasion and intracellular survival of bordetella bronchiseptica in mouse dendritic cells. | we have studied the interaction between the respiratory pathogen bordetella bronchiseptica and murine spleen dendritic cells, important antigen-presenting cells that are found in the airway epithelium. wild-type b. bronchiseptica 5376 attached very efficiently to dendritic cells, whereas the bvg mutant atcc 10580, wild-type strain bb7865, and its spontaneous delta bvgs mutant bb7866 bound less efficiently. however, all tested b. bronchiseptica strains were able to invade dendritic cells and surv ... | 1994 | 7960135 |
| mechanisms involved in uptake of bordetella bronchiseptica by mouse dendritic cells. | the invasion and intracellular survival of bordetella bronchiseptica in mouse dendritic cells were investigated. the results obtained suggest that b. bronchiseptica binds specifically to glycosylated receptors present on the plasma membrane of dendritic cells, thereby inducing a signal that triggers an actin polymerization-dependent phagocytic process, probably via a protein kinase-dependent transducing phosphorylation signal. the energy required for the uptake process by host cells is provided ... | 1994 | 7960136 |
| cloning of bordetella bronchiseptica urease genes and analysis of colonization by a urease-negative mutant strain in a guinea-pig model. | the genes encoding urease were cloned from bordetella bronchiseptica and the 5.2 kb of dna essential for expression analysed in a t7 rna polymerase transcription-translation system. at least four polypeptides with predicted molecular weights of 69,000, 26,000, 12,200 and 11,000 were found. partial dna sequence of the gene encoding the 69,000 da polypeptide revealed high amino acid identity to the alpha-subunit of proteus mirabilis urease, urec and jack bean urease. a stable, unmarked deletion wa ... | 1993 | 7968532 |
| tracheobronchial washings from seven vertebrate species as growth media for the four species of bordetella. | the four species of bordetella differed in their ability to grow at 37 degrees c in membrane-filtered tracheobronchial washings (tbw) from seven vertebrate species, including their natural hosts. from washed inocula of approximately 2 x 10(3) colony-forming units per ml (cfu ml-1), bordetella bronchiseptica and b. avium grew much better than the other two bordetellae and yielded stationary-phase cultures containing 10(8)-10(9) cfu ml-1 in most of the tbw samples. these counts were only moderatel ... | 1994 | 8004063 |
| epithelial cell invasion and survival of bordetella bronchiseptica. | wild-type bordetella bronchiseptica and a bvg mutant strain were used for invasion and survival experiments in human caco-2 and a549 epithelial cells. both bacterial strains were able to enter and persist within the host cells for at least a week. a significant proportion of the bacteria from both b. bronchiseptica strains but not from bordetella pertussis were found free in the cytoplasm, suggesting different invasion and survival strategies of the two species in epithelial cells. | 1994 | 8005690 |
| bordetella pertussis diagnosed by polymerase chain reaction. | the object of this work was to test the polymerase chain reaction (pcr) for demonstration of bordetella pertussis (bp) in nasopharyngeal secretions. the method was applied to patients with recently diagnosed pertussis, as verified by bp culture. in order to test the sensitivity and specificity of pcr for the diagnosis of bp, we used known concentrations of bp, bordetella parapertussis and bordetella bronchiseptica in aqueous solutions. pcr was furthermore carried out on species of bacteria that ... | 1994 | 8011307 |
| bvgas-mediated signal transduction: analysis of phase-locked regulatory mutants of bordetella bronchiseptica in a rabbit model. | members of the bordetella genus alternate between two distinct phenotypic phases in response to changes in their environment. this switch, termed phenotypic modulation, is mediated by the bvgas sensory transduction system. we developed an animal model based on the interaction of bordetella bronchiseptica with one of its natural hosts, the rabbit. to investigate the importance of bvgas signal transduction, we constructed constitutive (rb53) and bvg- (rb54) phase-locked derivatives of a wild-type ... | 1994 | 8039908 |
| [pneumonia caused by bordetella bronchiseptica]. | 1994 | 8049298 | |
| bordetella bronchiseptica dermonecrotizing toxin stimulates protein synthesis in an osteoblastic clone, mc3t3-e1 cells. | the effects of bordetella bronchiseptica dermonecrotizing toxin on protein synthesis in an osteoblastic clone, mc3t3-e1 cells, were investigated. the rate of protein synthesis in the serum-starved cells was increased by the toxin after a latent period of about 4 h, and reached 2.5 times that of the control 24 h after addition of toxin. the toxin raised the level of protein synthesis even in actively proliferating cells. the stimulatory effect of the toxin on protein synthesis occurred earlier th ... | 1994 | 8056289 |
| bordetella bronchiseptica pneumonia in a patient with aids. | bordetella bronchiseptica is recognised as a respiratory tract pathogen in many mammalian species, but has rarely been implicated in human infection. a case is reported of pneumonia caused by b bronchiseptica in a patient suffering from acquired immunodeficiency syndrome (aids). | 1994 | 8066571 |
| preparation of anti-canine serum amyloid a (saa) serum and purification of saa from canine high-density lipoprotein. | antiserum to canine serum amyloid a (saa) was prepared in rabbits by immunization with crude saa which was prepared from high-density lipoprotein 3 (hdl3) obtained from canine acute-phase serum. the antiserum was absorbed for contaminating antibodies by affinity chromatography using sepharose 4b coupled with normal canine serum proteins. the rabbit anti-canine saa serum reacted with a protein and formed a single precipitin line at the position of the alpha 1-region of the immunoelectrophoresis o ... | 1994 | 8066995 |
| detection and subcellular localization of three ptl proteins involved in the secretion of pertussis toxin from bordetella pertussis. | the ptl locus of bordetella pertussis contains eight open reading frames which are predicted to encode proteins (ptla to ptlh) that are essential for secretion of pertussis toxin from the bacterium and which are members of a family of transport proteins found in other types of bacteria. we have detected ptle, ptlf, and ptlg in immunoblots of extracts of b. pertussis by using antibodies raised to fusion proteins consisting of maltose-binding protein and the individual ptl proteins. these proteins ... | 1994 | 8071211 |
| detection of specific systemic and local igg and iga antibodies of pigs after infection with bordetella bronchiseptica by elisa. | an enzyme linked immunosorbent assay (elisa) for detection of igg and iga antibodies against bordetella bronchiseptica in serum and nasal secretions of pigs was developed. the elisa that used formolized phase i organisms of b. bronchiseptica as an antigen detected antibodies to a capsular k antigen(s) of the organism. in pigs which had an agglutinin titer of less than 10 and an elisa value of 0.47, on average, of maternal antibodies to b. bronchiseptica, igg antibody in serum increased 4 weeks o ... | 1994 | 8075212 |
| a rare case of infection of the lower respiratory tract caused by bordetella bronchiseptica. | 1994 | 8087129 | |
| invasion of hela cells by bordetella bronchiseptica. | invasion, defined as adhesion to, followed by entrance into hela cells by bordetella bronchiseptica was determined by (i) specific staining of intracellular bacteria and (ii) counting of viable intracellular bacteria after killing extracellular bacteria with colistin. it was demonstrated for the first time that b. bronchiseptica, like bordetella pertussis and bordetella parapertussis, is able to invade hela cells. comparison of the invasiveness of bvg+ and bvg- b. bronchiseptica showed that b. b ... | 1993 | 8099193 |
| fimbriae and determination of host species specificity of bordetella bronchiseptica. | a monoclonal antibody, designated cf8 and prepared against fimbrial protein enrichments of bordetella bronchiseptica 110h, was determined by immunogold electron microscopy to bind to some but not all fimbrial filaments on intact bacterial cells. comparison of the reactivity of this antibody with that of monoclonal antibody bpf2, which is specific for bordetella pertussis serotype 2 fimbriae, indicated that cf8 recognizes an epitope similar to that recognized by bpf2. by western blot (immunoblot) ... | 1993 | 8102377 |
| isolation of a putative fimbrial adhesin from bordetella pertussis and the identification of its gene. | we report the purification of a minor bordetella pertussis fimbrial subunit, designated fimd, and the identification of its gene (fimd). fimd could be purified from the bulk of major fimbrial subunits by exploiting the fact that major subunit-subunit interactions are more stable in the presence of sds than minor-major subunit interactions. to locate the gene for fimd, internal peptides of fimd were generated, purified and sequenced. subsequently, an oligonucleotide probe, based on the primary se ... | 1993 | 8105363 |
| chick embryo, a model to study the lethal activity of pertussis toxin, infectivity of bordetella pertussis, and their neutralization by immune sera. | the toxin activity of bordetella strains and acellular pertussis components was evaluated in chick embryos. eleven-day-old embryos were found to be most suitable for determination of ld50 values. eight of eight bordetella pertussis strains possessed ld50 values of 10(4) to 10(6) colony-forming units per dose of 100 microl. embryos were resistant to bordetella parapertussis and bordetella bronchiseptica at doses of 10(10) colony-forming units. bacterial growth did not occur in the bloodstream or ... | 1993 | 8106134 |
| immunogens of pasteurella. | the family pasteurellaceae pohl contains gram-negative, facultatively anaerobic and fermentative bacteria of the genera pasteurella, haemophilus, and actinobacillus. approximately 20 different species of the genus pasteurella have been identified using phenotypic and genetic analyses. of these species, p. multocida and p. haemolytica are the most prominent pathogens in domestic animals causing severe diseases and major economic losses in the cattle, swine, sheep, and poultry industries. mechanis ... | 1993 | 8116191 |
| bactericidal activity in the pig roundworm ascaris suum. | a potent, humoral, bactericidal activity against micrococcus luteus was discovered in pseudocoelomic fluid of the pig roundworm, ascaris suum. the activity, which was not bacteriolytic, was not due to lysozyme or to a dietary antibiotic. it was not inactivated by exposure to 100 degrees c, to low or high ph, or to ethanol. dialysis, electrophoresis and agar-diffusion experiments suggested that the main antibacterial activity in the fluid was associated with a basic substance of molecular weight ... | 1994 | 8144403 |
| feline bordetellosis: challenge and vaccine studies. | four eight-week-old cats, shown to be free from feline calicivirus, feline herpesvirus and chlamydia psittaci were challenged with an aerosol of bordetella bronchiseptica. within five days the cats developed signs of respiratory disease, characterised by nasal discharge, sneezing, spontaneous or induced coughing and dry or wet rales at auscultation. these signs were present for about 10 days, after which they began to resolve. to test the protective capacity of an experimental fimbrial antigen-b ... | 1993 | 8236648 |