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purification and subunit heterogeneity of pili of bordetella bronchiseptica.pili were isolated and purified from bordetella bronchiseptica. electron microscopic observations revealed that pili are ubiquitous in this species. the occurrence of pili and flagella appeared to correlate with growth phase and colonial morphology. pili were about 3 to 4 nm in diameter and morphologically similar to pili isolated from other gram-negative bacteria. internal core structure was not evident. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified pili showed that up t ...19862867974
characteristic cellular fatty acid composition and an ornithine-containing lipid as a new type of hemagglutinin in bordetella pertussis.the fatty acid composition of the total extractable cellular lipids of bordetella pertussis was very characteristic and was mostly hexadecenoic and hexadecanoic acids (90%) in a ratio of about 1:1. the fatty acid composition of bordetella parapertussis and bordetella bronchiseptica differed from that of b. pertussis. the two species were distinguished by the fatty acid composition of cell-bound lipids. the ornithine-containing lipid was characteristic of the genus bordetella and its main structu ...19852872110
bordetella adenylate cyclase: a genus specific protective antigen and virulence factor.most of the adenylate cyclase (ac) present in bordetella species is localized in the outer membrane, partly exposed to the cell surface. an isolation procedure to obtain the cell-bound enzyme was applied to bordetella bronchiseptica, bordetella pertussis and bordetella parapertussis. passive transfer of b. bronchiseptica anti-ac antibody, either in the form of monoclonal antibody or antibody transferred to offspring from female mice previously immunized with immunopurified b. bronchiseptica ac, ...19852872113
increase in intradermal vascular permeability caused by pertussis toxin from bordetella pertussis.rabbits that were injected intradermally with pertussis toxin (pt), produced from bordetella pertussis, showed slight edema and erythema at the injection sites, but not hemorrhage nor necrosis. the edema lesions were stained blue by the intravenous injection of pontamine sky blue 6b dye, suggesting that pt caused increased vascular permeability, similarly to the permeability factor (pf) of cholera toxin. the reaction of the pf of pt could be determined by measuring the diameter of the blue area. ...19872890084
evolutionary relationships in the genus bordetella.the nucleotide sequence of the pertussis toxin operon of bordetella pertussis, bordetella parapertussis and bordetella bronchiseptica, has shown that the last two species contain many common mutations and are likely to derive from a common ancestor (aricò and rappuoli, 1987). to elucidate further the evolutionary relationships between the bordetella species, we have cloned and sequenced the promoter region and the gene coding for the s1 subunit of pertussis toxin from additional b. pertussis str ...19872896289
positive regulation of pertussis toxin expression.although the genus bordetella contains several closely related species, pertussis toxin (pt) is produced only by phase i bordetella pertussis. in this work we have studied the regulation of expression of the pt operon and investigated why pt is produced by phase i and not by phase iii b. pertussis despite the presence of the pt genes. we have constructed a vector for bordetella species that contains the pt promoter fused to the coding region of the chloramphenicol acetyltransferase (cat) gene, a ...19882897691
[detection of the gene sequences of the leukocytosis (lymphocytosis)-stimulating factor of bordetella pertussis in bordetella parapertussis and bordetella bronchiseptica by using molecular hybridization].the 4.7 kb ecori-fragment of phase i b. pertussis 475 (serovar 1.2.3) chromosome dna carrying the pertussis toxin (pt) operon was cloned on vector plasmid puc19 in escherichia coli. three fragments (1.14 kb kpni-psti, 1.27 kb psti-psti, and 0.96 kb psti-psti) were obtained from the resulting hybrid plasmid, coded prh119, by electrophoretic techniques and used as a combined molecular probe for analysis of the ecori-digested and psti-digested chromosomal dna of b. pertussis strain 475 in phase i, ...19882901175
monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for detection of bordetella pertussis filamentous hemagglutinin.hybrid cell lines producing monoclonal antibodies against bordetella pertussis filamentous hemagglutinin (fha) were established. the specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay (elisa), sandwich elisa, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroblotting. the monoclonal antibody-based sandwich elisa was developed for detection of b. pertussis fha. the assay had a detection limit of b. pertussis fha in concentrations rang ...19882903174
studies on the antigenic relationships of six adherent isolates of bordetella bronchiseptica.six isolates of bordetella bronchiseptica recovered from swine with atrophic rhinitis were studied. all hemagglutinated swine red blood cells, autoagglutinated in saline and showed fimbriae by electron microscopy. hyperimmune sera against each were produced in rabbits and the antigenic relationships between the isolates were studied by cross-absorption and by the determination of the cross-reactivity indices of pairs of sera. three isolates seemed to be identical by both methods, while 2 others ...19882906770
characterization of fimbrial subunits from bordetella species.using antisera raised against serotype 2 and 3 fimbrial subunits from bordetella pertussis, serologically related polypeptides were detected in bordetella bronchiseptica, bordetella parapertussis and bordetella avium strains. the two b. pertussis fimbrial subunits, and three of the serologically related b. bronchiseptica polypeptides, were shown to be very similar in amino acid composition and n-terminal amino acid sequence. homology was observed between the n-termini of these polypeptides, and ...19872907088
structural and functional properties of a 69-kilodalton outer membrane protein of bordetella pertussis.a-69-kda outer membrane protein present on virulent bordetella pertussis cells is recognized by the agglutinating monoclonal antibodies bpe3, bpd8, and bpe8. the amino acid composition of this protein, purified from heat extracts of b. pertussis bp353 cells, is different from that of the two major fimbrial antigens of b. pertussis, which is consistent with its being a nonfimbrial protein based on other criteria. western blot analysis using the monoclonal antibody bpe3 demonstrated that a slightl ...19882908522
an evaluation in pigs of nobi-vac ar and an experimental atrophic rhinitis vaccine containing p multocida dnt-toxoid and b bronchiseptica.the trial involved eight large white sows obtained from a closed experimental specific pathogen free herd. four sows (two each for an experimental vaccine and for nobi-vac ar) were vaccinated twice (eight weeks and two weeks before parturition) with 2 ml of vaccine administered intramuscularly. two unvaccinated sows were used as an infected control group and two unvaccinated sows served as an uninfected control group. forty-six piglets (28 from vaccinated sows and 18 from unvaccinated sows) were ...19892919495
evaluation of bordetella bronchiseptica vaccines in specific-pathogen-free piglets with bacterial cell surface antigens in enzyme-linked immunosorbent assay.the progenies of specific-pathogen-free sows which had been immunized with bordetella bronchiseptica vaccines of various origin before parturition were challenged intranasally with b. bronchiseptica within 5 days of birth. sera of piglets were taken weekly and investigated by enzyme-linked immunosorbent assay against a mixture of b. bronchiseptica cell surface antigens containing curled fibers and fimbriae, lipopolysaccharide, and a mixture of proteins mostly derived from the outer membrane. the ...19852931379
lesions induced in the nasal turbinates of neonatal pigs inoculated with pasteurella multocia and/or bordetella bronchiseptica. 19862940398
a bovine haemagglutinin of bordetella bronchiseptica responsible for adherence. 19852940833
investigation of adhesive and nonadhesive bordetella bronchiseptica strains in a suckling-mouse model. 19852940834
changes in the nasal mucosa of specific-pathogen-free neonatal pigs infected with pasteurella multocida or bordetella bronchiseptica. 19862945021
prevention of atrophic rhinitis in piglets by means of intranasal administration of a live non-ar-pathogenic bordetella bronchiseptica vaccine.the effect of intranasal vaccination of piglets with live non-ar-pathogenic bordetella bronchiseptica (bb-) against atrophic rhinitis (ar) was investigated in a preliminary investigation and in a field trial. in the preliminary investigation 2-day-old spf piglets (n = 13) were vaccinated. three weeks after vaccination, challenges were carried out by means of a spray with an ar-pathogenic b (bb+) or an ar-pathogenic pasteurella multocida (pm+) broth-culture. four weeks later the piglets were necr ...19872956754
epizootiological studies on porcine atrophic rhinitis. xv. results of local immunization with a live bordetella bronchiseptica strain in a large-scale pig herd affected by atrophic rhinitis. 19882978213
adjuvant enhancement of humoral immune response to chemically inactivated bovine viral diarrhea virus.potentiation of the antibody response to inactivated bovine viral diarrhea virus by immunological adjuvants was studied in guinea pigs and cattle. the inactivated bovine viral diarrhea virus alone was demonstrated to be a weak immunogen. addition of either 2 mg per ml diethylaminoethyl-dextran or 5% alhydrogel to inactivated bovine viral diarrhea virus did not or only slightly stimulated the antibody response; the combined adjuvants induced a significantly higher titer. a higher concentration (2 ...19852985217
[diagnostic problems posed by respiratory infections of dogs].the following viruses as well as bacteria and mycoplasma have been isolated from dogs with contagious respiratory disease: canine distemper virus; canine adenoviruses (type 1 and 2); parainfluenza type 2 (sv5); reovirus type 1; canine herpesvirus; bordetella bronchiseptica, streptococcus, pasteurella, staphylococcus and mycoplasma. the occurrence of these agents can be in direct relationship with: the evolution of a systemic disease; respiratory disorders being a regular or inconsistant symptom ...19852998696
bordetella heat-labile toxin: further purification, characterization and mode of action.the heat-labile toxin (htl) was purified from sonic extracts of bordetella bronchiseptica and bordetella pertussis cells by a series of hydrophobic interactions, density gradient centrifugation, gel filtration, isoelectric precipitation, and isoelectric focusing. a 114-fold purification was regularly obtained with a yield of 31%. a dose of 0.75 ng was dermonecrotizing in guinea pigs. hlt is a simple protein (pi 6.9) with a molecular weight by gel filtration of 102,000 which consists of two polyp ...19853011569
plasmids related to rsf1010 from bordetella bronchiseptica.six out of 14 bordetella bronchiseptica isolates from u.k. pigs each contained one plasmid, of 8.7-44 kb. all plasmid-containing isolates were sulfonamide resistant, and this property was shown to be plasmid-encoded. five of the plasmids were related; two were indistinguishable from the broad-host-range plasmid, rsf1010. the other three, two of which appeared to be identical, were shown to have regions of homology with rsf1010. one of these regions encompassed the sulfonamide resistance determin ...19863012614
[isolation of a restriction endonuclease with hindiii-specificity from bordetella bronchiseptica].site-specific restriction endonuclease bbri has been found in bacteriophage resistant strain b. bronchioseptica 4994. the technique was elaborated for purification of bbri to the stage free of nuclease and phosphatase contamination. the yield of purified enzyme is 6000-20 000 units per 10 g of biomass. bbri recognises and cleaves the same dna sequence as hindiii with the formation of four-nucleotide cohesive ends. the simplicity of cultivation, security for human, presence of the single restrict ...19863025698
a survey of streptococcus pneumoniae, streptococcus zooepidemicus, salmonella spp., bordetella bronchiseptica and sendai virus in guinea pig colonies in japan.s. pneumoniae, s. zooepidemicus, salmonella spp., b. bronchiseptica and sendai virus were examined in a total of 45 guinea pig colonies (17 institutional and 28 breeder's colonies) of hartley strain, and found to be positive in 6, 3, 5, 20 and 14 colonies, respectively. sendai virus was highly prevalent among guinea pigs, showing so high rates positive as 80 to 100% of animals obtained from 11 of the 14 contaminated colonies. b. bronchiseptica was also shown to be prevalent within contaminated c ...19863026817
evidence for sialyl glycoconjugates as receptors for bordetella bronchiseptica on swine nasal mucosa.the nature of the receptors for bordetella bronchiseptica was investigated by using the in vitro adherence assay system. the results indicated that sialyl glycoconjugates acted as receptors on swine nasal mucosa. these results were obtained by two independent approaches: inhibition of epithelial cell adherence with sialic acid-containing compounds but not with compounds lacking sialic acid residues and loss of adherence after treatment of epithelial cells with periodate or neuraminidase. b. bron ...19873036708
[aerobic bacterial flora of the nasal cavity of rabbits].on the basis of bacteriological examinations carried out in april 1984 on 60 intranasal swabs, aerobic respiratory microbes were studied in rabbits. differences in flora between animals with and without respiratory diseases were studied. fourteen bacterial species were identified with no difference due to the pathological status. they were: bordetella bronchiseptica, staphylococcus epidermidis, streptococcus faecalis, pasteurella multocida, staphylococcus aureus, bacillus sp., branhamella catarr ...19863096189
electron microscopic observations on tracheal epithelia of mice infected with bordetella bronchiseptica.to clarify the pathogenesis of bordetella in vivo infection, the tracheal epithelia of mice were examined in detail by electron microscopy at various intervals after intranasal inoculation with graded doses of phase i bordetella bronchiseptica. in mice infected with a lethal dose (6 to 7 x 10(7) cfu), a remarkable rupture of the cell membranes of cilia and microvilli of the middle trachea was found on day i postinfection. the rupture of the membrane was observed over the entire tracheal epitheli ...19883173144
[the use of ultrasound-treated amd complete bordetella bronchiseptica antigens in an indirect enzyme immunoassay]. 19883178398
isolation and characterization of mutant strains of bordetella bronchiseptica lacking dermonecrotic toxin-producing ability.mutant strains of bordetella bronchiseptica, named b-42, b-76, b-84, and b-119, were obtained after serial passages of a parent strain, l3, on bordet-gengou agar plates containing 20% horse blood and 200 micrograms of nalidixic acid per ml (bgn-20 agar plates) at 42 degrees c. mutant strains completely lacked dermonecrotic toxin-producing ability, and lethal activity of the strains for mice was apparently reduced compared with that of strain l3. mutant strains were able to grow at 42 degrees c, ...19883182989
the pathogenesis of turbinate atrophy in pigs caused by bordetella bronchiseptica.the pathogenicity of 3 strains of bordetella bronchiseptica designated b58, pv6 and b65 was compared by intranasal infection of gnotobiotic piglets. strain b58 was a phase 1 isolate that produced haemolysin, an adhesin for calf erythrocytes, adenylate cyclase, mouse lethal factor, dermonecrotic factor and cytotoxin. b65 was a variant of b58 that produced no detectable haemolysin, adhesin or adenylate cyclase and 10-fold smaller amounts than b58 of mouse lethal factor, dermonecrotic factor and cy ...19883218075
[the differentiation of bordetella bronchiseptica strains. 5. virulence factors in relation to phase changes in bordetella bronchiseptica strains]. 19883219030
[the differentiation of bordetella bronchiseptica strains. 6. detection of adhesion in bordetella bronchiseptica]. 19883219031
in vitro susceptibility of bacteria to a ticarcillin-clavulanic acid combination.in vitro testing of bacterial susceptibility to a combination of ticarcillin and clavulanic acid was done, using 406 aerobic gram-positive and gram-negative isolates (considered to be pathogens) cultured from equine and small animal specimens. a microdilution broth technique of susceptibility testing was performed, using trays with wells containing a range of doubling concentrations of dehydrated ticarcillin (range, 0.50 to 128 micrograms/ml) with fixed concentration of clavulanic acid (4 microg ...19883239838
decrease in antimicrobial susceptibility of bordetella bronchiseptica caused by antigenic modulation and phase variation.the susceptibilities to 20 antimicrobial agents of bordetella bronchiseptica variants in c mode and degraded phases were compared with those of their parents in x mode. increases in mics of 4- to 32-fold were observed for ampicillin, carbenicillin, erythromycin, novobiocin, rifampin, chloramphenicol, nalidixic acid, and oxolinic acid.19883245700
induction of nasal turbinate atrophy in germ-free pigs, using pasteurella multocida as well as bacterium-free crude and purified dermonecrotic toxin of p multocida.to establish the role of the dermonecrotic toxin (dnt) of pasteurella multocida in the cause and pathogenesis of atrophic rhinitis, germ-free pigs were inoculated with several strains of p multocida, crude dnt, or purified dnt. in some experiments, the aforementioned inocula were combined with bordetella bronchiseptica. all dnt-producing p multocida strains induced severe turbinate atrophy. histologic examination of the remnants of the nasal turbinates revealed intact, but undulated, ciliated ep ...19883247905
electron microscopic observations of ciliated epithelium of mouse trachea treated in vitro with a membrane-damaging factor from bordetella bronchiseptica. 19883253469
adherence of pasteurella multocida or bordetella bronchiseptica to the swine nasal epithelial cell in vitro.the interaction of bordetella bronchiseptica or pasteurella multocida with swine nasal epithelial cells was studied in vitro. the mean number of b. bronchiseptica organisms adhered per cell was about three times as high as that of p. multocida (p less than 0.01), and the adherence was specifically inhibited by the homologous antiserum prepared with the whole-cell antigen of each bacterium. the poor affinity of p. multocida to the swine nasal mucosa as compared with that of b. bronchiseptica was ...19883335403
development of turbinate lesions and nasal colonization by bordetella bronchiseptica and pasteurella multocida during long-term exposure of healthy pigs to pigs affected by atrophic rhinitis.natural transmission of atrophic rhinitis from pigs from a herd with an endemic atrophic rhinitis problem to pigs from a herd free of atrophic rhinitis was demonstrated. six replicates each with five pigs from the endemic atrophic rhinitis herd (group a) and five pigs from the atrophic rhinitis-free herd (group b) were housed together from 5 wk of age, with each replicate kept in isolation rooms maintained at optimal and controlled environmental conditions. three replicates each with six pigs/ro ...19883349398
adherence of bordetella bronchiseptica and pasteurella multocida to porcine nasal and tracheal epithelial cells.the ability of 19 different bordetella bronchiseptica isolates and 25 pasteurella multocida isolates to adhere in vitro to porcine nasal and tracheal epithelial cells was examined. it was found that b. bronchiseptica adhered well to upper respiratory tract cells. in contrast the number of p. multocida organisms which adhered was four to six times less than the number of b. bronchiseptica adherent organisms. this difference was statistically significant (p less than 0.0001). both microorganisms a ...19883370564
bovine erythrocyte-agglutinin as a possible adhesin of bordetella bronchiseptica responsible for binding to porcine nasal epithelium.the bovine erythrocyte-agglutinin (bea) of bordetella bronchiseptica, located on the cell surface in non-fimbrial form, has been identified as a possible adhesion responsible for binding to porcine nasal epithelium in studies with bea-negative (bea-) mutants and in competitive studies with bovine erythrocytes.19883392727
[the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase]. 19883395180
effect of antigenic modulation and phase variation on adherence of bordetella bronchiseptica to porcine nasal epithelial cells.in parallel with bacterial density, bordetella bronchiseptica phase-i, x-mode cells adhered well to porcine nasal epithelial cells, whereas c-mode cells and variant cells in phases ii, iii, and rough adhered feebly. cell surface components lost, on transition from x to c mode or from phase i to degraded phases, may confer much of the organism's adherence ability.19873434914
the primary and secondary cellular immune responses to whole cell bordetella pertussis vaccine and its components.the cellular immune responses of balb/c mice and wistar rats immunized in hind footpads with intact killed bordetella pertussis were found to differ from those of similar animals immunized with other bacteria including bordetella bronchiseptica, salmonella typhimurium and escherichia coli. all the bacteria stimulated increases in cell number, proliferation and interleukin 2 (il-2) production in popliteal lymph nodes which peaked 3-5 days after injection and decreased to resting levels by day 7. ...19873498569
an evaluation of the api zym system as a means of identifying haemophilus somnus and related taxa.the commercially available api zym microbiological identification system was evaluated for the rapid identification of haemophilus somnus. eighty-seven isolates of the organism had api zym profiles which were characteristic. the api zym profiles demonstrate clear differences between h. somnus and other genera but suggest a close association to three related organisms. enzyme activity of h. somnus isolates were similar to organisms identified as histophilus ovis, haemophilus agni and strains uqv ...19863530416
bordetella bronchiseptica and toxigenic type d pasteurella multocida as agents of severe atrophic rhinitis of swine.bordetella bronchiseptica and toxigenic type-d pasteurella multocida were cultured from pigs in each of five herds diagnosed as having severe atrophic rhinitis (ar). b. bronchiseptica alone, p. multocida alone, or both organisms isolated from four herds were inoculated intranasally into 1-week-old gnotobiotic pigs which were necropsied 4 weeks post-inoculation (pi). nasal turbinate atrophy in b. bronchiseptica-inoculated pigs was moderate to severe, while p. multocida-inoculated pigs had slight ...19873564360
bordetella bronchiseptica bronchitis.the clinical course of a patient with bronchitis caused by bordetella bronchiseptica is described. the organism was recovered on one occasion from a protected catheter brush specimen obtained at bronchoscopy and on two occasions from expectorated sputum specimens. the infection was eradicated with antimicrobial therapy.19873571462
effect of purified heat-labile toxin of bordetella bronchiseptica on the peripheral blood vessels in guinea pigs or suckling mice. 19863574159
clonal diversity and host distribution in bordetella bronchiseptica.a total of 303 isolates of bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. the population structure of b. bronchiseptica is clonal, and its genetic diversity is limited in comparison with most other pathogenic bacteria, perhaps reflecting a relatively recent origin of the speci ...19873584070
bordetella parapertussis and bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes.pertussis toxin, the major virulence factor of bordetella pertussis, is not produced by the closely related species bordetella parapertussis and bordetella bronchiseptica. it is shown here that these two species possess but do not express the complete toxin operon. nucleotide sequencing of an ecori fragment of 5 kilobases comprising the regions homologous to the pertussis toxin genes shows that in this region, b. parapertussis and b. bronchiseptica are 98.5% and 96% homologous, respectively, to ...19873584073
a note on the isoprenoid quinone content of bordetella avium and related species.the isoprenoid quinone content of isolates of bordetella avium (four strains), alcaligenes faecalis (one strain), bordetella bronchiseptica (one strain) and a bordetella avium-like organism (four strains) was determined by reverse-phase high-performance liquid chromatography. all the isolates contained ubiquinones with eight isoprene units as the major component. no menaquinones were detected.19873597205
differentiation of bordetella avium and related species by cellular fatty acid analysis.the fatty acids of 18 strains of bordetella avium, 3 strains of alcaligenes faecalis, 5 strains of bordetella bronchiseptica, and 12 strains of a b. avium-like organism were examined by gas chromatography-mass spectrometry. the presence of a significant amount of the acid 2-oh c14:0 characterized b. avium and the b. avium-like organism. b. avium and the b. avium-like organism differed in their relative concentrations of c16:1 and 3-oh c14:0 acids. b. bronchiseptica and a. faecalis were distingui ...19873597749
efficacy of commercial vaccines for protecting guinea pigs against bordetella bronchiseptica pneumonia.autogenous bacterins are recommended to protect guinea pigs (cavia porcellus) against pneumonia due to bordetella bronchiseptica. bordetella vaccines are available commercially for several other animal species. the substantial antigenic cross-reactivity among bordetella isolates from various animal species suggests that immunity resulting from use of these vaccines might protect guinea pigs. groups of ten individually housed hartley guinea pigs from a colony free of bordetella were vaccinated wi ...19873599889
[cultivation of bordetella bronchiseptica in nutrient media with known amino acid content]. 19873606325
a rapid enzyme-linked semi-microwell assay for the enumeration of antibody-forming cells to viral and bacterial antigens in domestic animals.details of an enzyme immunoassay for the enumeration of antibody-forming cells are given with examples of its application to immunization with viral (newcastle disease virus) or bacterial (haemophilus pleuropneumoniae) pathogens of domesticated species. the assays showed reciprocal specificity when compared to another virus (avian paramyxovirus serotype 3) or another bacterium (bordetella bronchiseptica) and were used to show the class specificity of avian afc and the effect of passive antibody ...19873611797
stimulation of bone resorption by inflamed nasal mucosa, dermonecrotic toxin-containing conditioned medium from pasteurella multocida, and purified dermonecrotic toxin from p. multocida.the effects of inflamed nasal mucosa from pigs with atrophic rhinitis (ar), cell extract from bordetella bronchiseptica, conditioned medium from pasteurella multocida, and purified dermonecrotic toxin (dnt) from p. multocida on mouse fetal long bones in organ culture were studied. inflamed nasal "ar mucosa" stimulated the release of 45ca from prelabeled cultures, while histologically the formation of calcified matrix was impaired as well. b. bronchiseptica cell extract only transiently increased ...19873623695
airborne-induced experimental bordetella bronchiseptica pneumonia in strain 13 guineapigs.to evaluate the efficacy of a commercial bacterial vaccine in protecting strain 13 guineapigs against fatal bordetella bronchiseptica pneumonia, it was necessary to establish the infectivity and disease pathogenesis induced by virulent organisms. when guineapigs were exposed to small-particle aerosols of varying concentrations of virulent b. bronchiseptica, a spectrum of disease was produced that ranged from inapparent illness to fulminant bronchopneumonia. clinical signs began by day 4 after ex ...19873626470
bordetella bronchiseptica pneumonia in a patient with chronic lymphocytic leukemia.this case report describes two episodes of pneumonia caused by bordetella bronchiseptica in a patient with chronic lymphocytic leukemia. there was discrepancy between the in vitro sensitivity testing of the organism and subsequent clinical response to several antimicrobial agents. human infection with b bronchiseptica is almost always associated with severe underlying disease and contact with an appropriate animal reservoir.19873629325
[suitability of laboratory mouse strains for virulence testing of bordetella bronchiseptica]. 19873632239
[comparative local and systemic immunization against experimentally-induced bordetella bronchiseptica pneumonia in icr mice].it is described the experimental bordetella bronchiseptica infection via aerosol in icr mice. 10(6) living bacteria were found in the lung after aerosol challenge with a total of 10(12) cfu. the pneumonias produced in this infection model within 10 days are focal, interstitial and proliferative to a low and medium degree, respectively. they are significantly reduced already by a single i.p. or s.c. immunization. two vaccinations with inactivated bordetellae or a temperature sensitive mutant redu ...19873687105
isolation and characterisation of bordetella avium and related species and an evaluation of their role in respiratory disease in poultry.a total of 24 gram negative non fermentative bacteria obtained from poultry were compared with reference strains of bordetella avium, alcaligenes faecalis, bordetella bronchiseptica and a bordetella avium-like organism. thirteen isolates were identified as b. avium and 11 were identified as b. avium-like. a commercial microidentification kit (the api2one) did not identify the field isolates but did separate them correctly into the 2 groups. a practical identification scheme, suitable for diagnos ...19873689262
pharmacokinetics of cefazolin in guinea pigs.the occurrence of antibiotic-related enterocolitis in guinea pigs restricts the use of many common antibiotics in this species. cephaloridine, an antibiotic frequently recommended for this species, is no longer available and a substitute has yet to be explored. in this study, the potential therapeutic efficacy of cefazolin, also a first generation cephalosporin, was evaluated in guinea pigs by assessing pharmacokinetics, toxicity and the minimal inhibitory concentration for selected animal patho ...19873695403
properties of dermonecrotic toxin prepared from sonic extracts bordetella bronchiseptica.a toxin with dermonecrotic activity (dnt) was purified from sonic extracts of bordetella bronchiseptica l3 of pig origin at phase i by chromatographic and electrophoretic methods. the purification procedure was one developed for obtaining the pasteurella multocida dnt from sonic extracts with some modifications. dermonecrotizing activity of b. bronchiseptica-purified dnt was increased by 600-fold compared with that of the crude extract, and the average yield was about 3%. the toxin was homogeneo ...19863699886
bordetella bronchiseptica phase variation induced by crystal violet.a method for effective induction of phase variation in bordetella bronchiseptica by treatment with crystal violet (cv) is presented. when grown in cv-broth, phase i cells dissociated into three serial phases. appearance of variant cells was observed simultaneously with the beginning of cell multiplication. the maximum effect of cv was obtained at a concentration of 8 micrograms/ml, when the proportion of variants in the population reached 100%. the main factors which affected phase variation wer ...19863700613
[differentiation of bordetella bronchiseptica strains. 4: pathogenetic significance of heat-labile dermonecrotoxins of bordetella bronchiseptica for the development of atrophic rhinitis in baby mice]. 19863729653
contribution to the taxonomy of the turkey coryza agent: cellular fatty acid analysis of the bacterium.gas-liquid chromatography was used to analyze bacterial cellular fatty acids to elucidate the relatedness of the turkey coryza (tc) bacterium to alcaligenes spp., bordetella spp., and other gram-negative bacteria. the results indicated that the tc bacterium is not closely related to alcaligenes faecalis or any of the reference strains of alcaligenes and bordetella studied. most urease-positive bacterial isolates obtained from the upper respiratory tract of turkeys were identified as bordetella b ...19863729861
functional and ultrastructural changes in alveolar macrophages from rabbits colonized with bordetella bronchiseptica.alveolar macrophages from rabbits colonized with bordetella bronchiseptica in their respiratory tract exhibited significant decreases in cell adherence, phagocytic uptake, and bactericidal activity compared with macrophages from uncolonized animals. these dysfunctions were accompanied by ultrastructural changes, including a decrease in overall cell density, a vacuolation of the rough endoplasmic reticulum, and an increase in organelle-poor cell surface projections.19863744561
the synergistic activity of tiamulin and chlortetracycline: in-feed treatment of bacterially complicated enzootic pneumonia in fattening pigs.the antibacterial effects of a combination of tiamulin and chlortetracycline in vitro against a number of field isolates of pasteurella multocida, haemophilus pleuropneumoniae and bordetella bronchiseptica were examined. there was a marked synergism between the two antibiotics against all eight isolates of p multocida, against seven of nine isolates of h pleuropneumoniae and against the single strain of b bronchiseptica tested. two field trials were carried out on a herd with a history of compli ...19863750792
investigation into the pathogenesis of atrophic rhinitis in pigs. i. atrophic rhinitis caused by bordetella bronchiseptica and pasteurella multocida and the meaning of a thermolabile toxin of p. multocida.in two groups of swine herds, herds with and without clinical ar the presence of atrophic rhinitis (ar) correlated with the presence of toxinogenic pasteurella multocida (pm) and not with the bordetella bronchiseptica (bb) infection. six bb- and eighteen pm-strains have been investigated for ar pathogenicity. broth cultures were injected intradermally in guinea-pigs (gpst) or intranasally in 3-week-old colostrum deprived specific pathogen free (spf) piglets. the average atrophy of the ventral co ...19863750802
purification and characterization of heat-labile toxin from bordetella bronchiseptica.the heat-labile toxin (hlt) of bordetella bronchiseptica was purified successively from sonic extracts of phase i organisms grown in stainer-scholte medium, by partition in hydrophobic interaction, sucrose density gradient centrifugation, gel filtration through sepharose 4b and 6b, isoelectric precipitation and isoelectric focusing. the purified hlt was homogeneous by disc polyacrylamide gel electrophoresis and the gel diffusion-test, and free of detectable hemagglutinin and endotoxin activity. ...19863773790
induced atrophic rhinitis in rats.infections with bordetella bronchiseptica and pasteurella multocida were inducted in newborn specific-pathogen-free rats. turbinate atrophy was quantified by measuring the length of the osseous core of the ventral turbinates. bordetella bronchiseptica readily colonized the nasal cavity. inoculated rats developed serum agglutinating antibodies to b bronchiseptica. turbinate atrophy, correlating with a severe inflammatory reaction, was observed after dual inoculation with b bronchiseptica or after ...19863789506
virulence factors of bordetella bronchiseptica associated with the production of infectious atrophic rhinitis and pneumonia in experimentally infected neonatal swine.four strains of bordetella bronchiseptica (csu-p-1, 64-c-0406, 1120-a-83-013, and b205bt) with defined virulence for neonatal swine were examined, and an attempt was made to correlate the presence of certain in vitro phenotypic characteristics with the ability of a particular b. bronchiseptica strain to produce turbinate and lung lesions in piglets. all of the strains except csu-p-1 colonized the nasal passages of the pigs heavily, and strains 1120-a-83-013 and b205bt produced moderate to severe ...19873793231
otitis media in guinea pigs: pathology and bacteriology.in the course of post-mortem examination of conventional random-bred and inbred (immunosuppressed) strain 2/n guineapigs kept in separate quarters, otitis media was diagnosed in 62 of 462 animals (13.4%) and 18 of 66 animals (27.3%) respectively. clinical signs of otitis media were seen in only two random-bred animals but in nearly 50% of affected inbred animals. in random-bred guineapigs, purulent, often bilateral, otitis media was associated mainly with the isolation of streptococcus zooepidem ...19863795863
nucleotide sequence homology to pertussis toxin gene in bordetella bronchiseptica and bordetella parapertussis.multiple strains of bordetella parapertussis and b. bronchiseptica were examined for the presence of nucleotide sequences which hybridized with a cloned 4.5-kilobase (kb) fragment of b. pertussis dna containing the genes responsible for pertussis toxin expression. all six b. parapertussis strains tested had nucleic acid sequences that hybridized with the cloned 4.5-kb fragment in southern blot analyses. when the b. parapertussis dna was digested with restriction endonuclease psti, the pattern of ...19873818081
[quantitative study of the sensitivity of bordetella bronchiseptica and pasteurella multocida to sulfonamides].information concerning the resistance to sulfonamides in the netherlands was obtained by determining the minimal inhibitory concentrations (mic's) of 119 strains of bordetella bronchiseptica and 151 strains of pasteurella multocida, obtained from pigs at five veterinary bacteriology centres, to sulfadimidine (sdm) and sulfamethoxazole (smx). the mic's of sdm against su-susceptible strains were usually 4 times higher than those of smx. about one third of b. bronchiseptica isolates were resistant ...19873824346
isolation of bordetella avium from poultry.four australian isolates of gram-negative nonfermentative bacteria obtained from poultry were compared with reference strains of bordetella avium, bordetella bronchiseptica and alcaligenes faecalis. the australian isolates were identified as bordetella avium. a routine procedure for the identification of this recently recognised poultry pathogen is described.19853834901
opsonic activity of mcp-1 and mcp-2, cationic peptides from rabbit alveolar macrophages.mcp-1 and mcp-2, cationic peptides derived from rabbit alveolar macrophages, enhanced the ability of these cells to ingest staphylococcus aureus, klebsiella pneumoniae, bordetella bronchiseptica, and candida albicans in vitro. the opsonic effect of mcp-1 was potentiated by ca++ and mg++ and was associated with binding of the peptide to alveolar macrophages and microorganisms. mcp-1 and mcp-2 may contribute to the ability of alveolar macrophage to ingest microorganisms that gain entry to the lowe ...19853888509
[atrophic rhinitis in swine: studies on the occurrence of toxin-producing strains of pasteurella multocida and bordetella bronchiseptica]. 19853902442
genetic diversity and relationships in populations of bordetella spp.genetic diversity in 60 strains of three nominal bordetella species recovered from humans and other mammalian hosts was assessed by analyzing electrophoretically demonstrable allelic variation at structural genes encoding 15 enzymes. eleven of the loci were polymorphic, and 14 distinctive electrophoretic types, representing multilocus genotypes, were identified. the population structure of bordetella spp. is clonal, and genetic diversity is relatively limited compared with most other pathogenic ...19863957867
identification of a 68-kilodalton protective protein antigen from bordetella bronchiseptica.a 68-kilodalton (kd) outer membrane protein antigen of bordetella bronchiseptica has been identified by using monoclonal antibodies that recognized two nonoverlapping determinants. antibody bb05 also reacted with homologous proteins from bordetella pertussis and bordetella parapertussis but not with another 12 organisms from various bacterial genera. passive injection of bb05 antibody protected mice from aerosol infection with b. bronchiseptica as shown by reduced mortality and reduced pathology ...19853972452
enzyme-linked immunosorbent assay for immunoglobulin g antibody to pasteurella multocida in rabbits.three antigen preparations of pasteurella multocida, lipopolysaccharide antigen, boiled-cell extract antigen, and boiled whole-bacterium antigen, were used in an enzyme-linked immunosorbent assay (elisa) to detect rabbit immunoglobulin g antibody to p. multocida. the sensitivity of each antigen preparation was compared by using sera from p. multocida-infected and uninfected rabbits and sera from two rabbits immunized with different serotypes of p. multocida. in the elisa, all three antigen prepa ...19853988903
is phase variation in bordetella caused by mutation and selection?in vitro growth conditions of bordetella bronchiseptica led to an enrichment of phase variants. the frequency of phase variation was about 10(-6) per cell per generation. therefore phase variation in bordetella may result from a random mutation in a controlling region, followed by selection.19853989513
[pathohistological research on the white mouse after infection with swine strains of bordetella bronchiseptica. 2. pathohistological research results using intratracheally infected mice]. 19853994488
[pathohistological research on the white mouse after infection with swine strains of bordetella bronchiseptica. 1. pathohistological research results using intravenously infected mice]. 19853994494
intracellular locations of dermonecrotic toxins in pasteurella multocida and in bordetella bronchiseptica.location of dermonecrotic toxin (dnt) in the cells of pasteurella multocida or bordetella bronchiseptica was investigated. after cell lysis by various procedures, various fractions prepared from bacterial cells grown in liquid culture media were assayed for dermonecrotic activity by skin testing of guinea pigs. during the death phase of the growth tested for the 2 bacterial species, little cell-free dnt was detected in the culture supernatants. throughout the log and stationary phases of the gro ...19854014837
[occurrence of sick animals in conventional guinea pigs obtained from commercial breeders during 1964 to 1982].a quarantine was performed on conventional hartley guinea pigs free from streptococcus zooepidemicus, bordetella bronchiseptica and salmonella spp., but infected with eimeria caviae, which were purchased from twenty-five commercial breeders by the national institute of health. physical examinations revealed an incidence of 0.88 per cent or 1,461 sick animals in 166, 050 guinea pigs quarantined during the period 1964 to 1982. the result obtained showed the following significant differences betwee ...19854018153
[prospective study of the presence of atrophic rhinitis and ar-pathogenic bordetella bronchiseptica and pasteurella multocida bacteria in piglets of health-certified pig-breeding farms].two hundred eight-week-old piglets from 20 pig-breeding farms, on which the clinical findings did not provide any justification for suspecting the presence of atrophic rhinitis (ar), were examined for this disease. clinical parameters were brachygnathia superior (bs) and snout-distorsion (kd). piglets with bs (n = 48) were observed on 13 farms and with kd on 8 farms. morbid anatomical parameters were deviation of the nasal septum (sd) and atrophy of the ventral conchae (avc). sd was observed in ...19854024056
adenylate cyclase activity of a 68,000-molecular-weight protein isolated from the outer membrane of bordetella bronchiseptica.a method was developed which is suitable for the isolation of substantial quantities of outer membrane proteins of bordetella species in a water-soluble form. the extracted material may then be further fractionated in the absence of detergents by ion-exchange chromatography and preparative flat-bed isoelectrofocusing. these procedures facilitated the isolation of one of the proteins, of molecular weight 68,000, for which the antibody titer correlated with the degree of protection against nasal c ...19854044033
[respiratory diseases of swine: some epidemiologic aspects].epidemiological surveys enable to know better the aetiology of respiratory diseases. the surveys carried out in slaughter-houses in brittany (france) in 1980 and 1981, show a worrying situation concerning porcine respiratory diseases. microbiological studies brought out the preponderance of the isolation of mycoplasma hyopneumoniae, pasteurella multocida, bordetella bronchiseptica, streptococcus suis and actinobacillus suis. some patterns of experimental infection with mycoplasma hyopneumoniae a ...19854064626
[significance of bordetella bronchiseptica and pasteurella multocida in rhinitis atrophicans suum]. 19854072490
[occurrence of bordetella avium sp. nov. and bordetella bronchiseptica in birds]. 19854074318
[selective isolation of bordetella bronchiseptica (author's transl)]. 19734147745
observations on commensal cultures of bordetella bronchiseptica and rat hepatoma cells. electron microscopy. 19674164679
electron microscopy of a strain of bordetella bronchiseptica.a strain of bordetella bronchiseptica that had been isolated from a rat hepatoma cell culture was investigated by means of electron microscopy. bacteria were examined after (i) negative staining with phosphotungstate or uranyl acetate, (ii) metal shadowing with platinum-palladium, and (iii) fixation with glutaraldehyde followed by embedding, sectioning, and staining. the multilayered bacterial cell walls appeared lobulated in negatively stained and in metal-shadowed specimens; the lobules were d ...19674167588
comparison of pathogenicity of various isolates of bordetella bronchiseptica in young pigs.eight groups of 12-to 24-hour-old pigs were procured from a respiratory disease-free herd of swine and reared in isolation using a box-rearing procedure. they were inoculated intranasally at 3 days of age with different isolates of bordetella bronchiseptica. it was found at necropsy 4 weeks post-inoculation that 4 isolates of swine origin, an isolate of rabbit origin and an isolate of cat origin caused mild to moderate turbinate atrophy in 22 of 24 pigs. an isolate of rat origin caused mild turb ...19674226661
[respiratory infection in the turkey caused by a bacterium related to bordetella bronchiseptica].a respiratory condition encountered in young turkeys is reported with the isolation of bordetella bronchiseptica as the possible etiological agent. rhinitis, bronchitis and bronchopneumonia were the main lesions caused by this bacteria. transmission trials were successful in turkeys and chicken but not in mice. guinea pigs were also infected for comparative pathological studies.19674226680
bordetella bronchiseptica isolated from a fatal case of bronchopnuemonia in an african green monkey. 19684233379
agglutinating antibodies for bordetella bronchiseptica in sera before, during, and after an epizootic of pneumonia in caged monkeys. 19704246021
pneumonia associated with bordetella bronchiseptica in callicebus species primates. 19704247042
the effect of medicated feed on the nasal microflora and weight gain of pigs.antimicrobial agents were added to the feed of swine for three weeks to determine the interrelationships of potentially pathogenic agents in the nasal tract, turbinate atrophy and weight gains. bordetella bronchiseptica was not isolated from the groups fed the combination of chlortetracycline, penicillin and sulfamethazine. b. bronchiseptica was found in some pigs after the feeding trail, but this organism was not significantly associated with turbinate atrophy at the time of slaughter.mycoplasm ...19724258545
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