Publications

serum antibody responses to vaccinal antigens in lean and obese geriatric dogs.	the immune responses in control dogs [1 to 4 years of age, body condition score (bcs): 4 to 5 out of 9] were compared to those of aging dogs (based on breed and body size) either categorized as lean (bcs: 4 to 5 out of 9) or obese (bcs: 8 to 9 out of 9). of interest were the serum titers to the following common agents found in vaccines, canine parainfluenza virus (cpiv), canine parvovirus (cpv), canine distemper virus (cdv), canine respiratory coronavirus (crcov), and bordetella bronchiseptica. ...	2016	27152043
pandemic h1n1 influenza virus infection in a canadian cat.	a cat was presented for necropsy after being found dead at home. histologic findings suggested viral pneumonia. polymerase chain reaction and viral typing revealed influenza a(h1n1)pdm09. this is the first report of influenza in a canadian cat and highlights the importance of considering influenza virus in the differential diagnosis for feline respiratory distress.	2016	27152036
bipa is associated with preventing autoagglutination and promoting biofilm formation in bordetella holmesii.	bordetella holmesii causes both invasive and respiratory diseases in humans. although the number of cases of pertussis-like respiratory illnesses due to b. holmesii infection has increased in the last decade worldwide, little is known about the virulence factors of the organism. here, we analyzed a b. holmesii isolate that forms large aggregates and precipitates in suspension, and subsequently demonstrated that the autoagglutinating isolate is deficient in bordetella intermediate protein a (bipa ...	2016	27448237
retrospective analysis of bacterial and viral co-infections in pneumocystis spp. positive lung samples of austrian pigs with pneumonia.	aim of this study was the retrospective investigation of viral (porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), torque teno sus virus type 1 and 2 (ttsuv1, ttsuv2)) and bacterial (bordetella bronchiseptica (b. b.), mycoplasma hyopneumoniae (m. h.), and pasteurella multocida (p. m.)) co-infections in 110 pneumocystis spp. positive lung samples of austrian pigs with pneumonia. fifty-one % were positive for pcv2, 7% for prrsv, 22% for ttsuv1, 48% for t ...	2016	27428002
partial purification and characterization of an antimicrobial activity from the wood extract of mangrove plant ceriops decandra.	the development of resistance towards the antibiotics in use today has been a source of growing concern in the modern healthcare system around the world. to counter this major threat, there is an urgent need for discovery of new antimicrobials. many plants, like mangroves, possess highly diversified list of natural phytochemicals which are known to have wide range of bioactivities. these phytochemicals can be good sources for the discovery of new drugs. in this study, we report the partial phyto ...	2016	27065777
btea secreted from the bordetella bronchiseptica type iii secetion system induces necrosis through an actin cytoskeleton signaling pathway and inhibits phagocytosis by macrophages.	btea is one of the effectors secreted from the bordetella bronchiseptica type iii secretion system. it has been reported that btea induces necrosis in mammalian cells; however, the roles of btea during the infection process are largely unknown. in order to investigate the btea functions, morphological changes of the cells infected with the wild-type b. bronchiseptica were examined by time-lapse microscopy. l2 cells, a rat lung epithelial cell line, spread at 1.6 hours after b. bronchiseptica inf ...	2016	26828590
rare detection of the acinetobacter class d carbapenemase blaoxa-23 gene in proteus mirabilis.		2016	26902769
safety and early onset of immunity with a novel european porcine reproductive and respiratory syndrome virus vaccine in young piglets.	porcine reproductive and respiratory syndrome virus (prrsv) can be difficult to manage in commercial settings. a novel type i prrsv vaccinal strain (94881) was evaluated for safety and efficacy/onset of immunity (ooi) in piglets. in 2 experiments, groups of piglets were vaccinated intramuscularly (im) at approximately 14 d of age with a maximum-range commercial dose, an overdose, or a placebo in experiment 1 and either a minimum-range commercial dose or a placebo in experiment 2. the piglets in ...	2016	27127339
isolation of campylobacter spp. from client-owned dogs and cats, and retail raw meat pet food in the manawatu, new zealand.	campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. the disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. the prevalence of campylobacter spp. in dogs and cats varies widely, and data on new zealand pets are limited. this study aimed to investigate the prevalence of campylobacter spp. in dogs, cats and retail raw meat pet food ...	2016	27860343
characterization of polymyxin b biodistribution and disposition in an animal model.	despite dose-limiting nephrotoxicity concerns, polymyxin b has resurged as the treatment of last resort for multidrug-resistant gram-negative bacterial infections. however, the pharmacokinetic, pharmacodynamic, and nephrotoxic properties of polymyxin b still are not thoroughly understood. the objective of this study was to provide additional insights into the overall biodistribution and disposition of polymyxin b in an animal model. sprague-dawley rats were dosed with intravenous polymyxin b (3 ...	2016	26643340
florfenicol as a modulator enhancing antimicrobial activity: example using combination with thiamphenicol against pasteurella multocida.	synergistic effects between the same class of antibiotics are rarely reported. our previous study found synergistic-like interaction between florfenicol (ffc) and thiamphenicol (tap) against staphylococcus aureus. here, the enhanced antimicrobial activity was evaluated in 97 clinical isolates of both gram-negative and gram-positive bacteria. susceptible strains were initially identified by checkerboard microdilution assay (fractional inhibitory concentration index [fici] ≤ 0.625), followed by co ...	2016	27065961
efficacy of mycoplasma hyopneumoniae vaccination before and at weaning against experimental challenge infection in pigs.	commercial bacterins are widely used at weaning to control mycoplasma hyopneumoniae infections in pigs. however, it is not known whether the efficacy of vaccinating against m. hyopneumoniae can be influenced by the weaning process when vaccination is applied at the day of weaning. the present study assessed the efficacy of a single m. hyopneumoniae vaccination (ingelvac mycoflex®) three days before weaning (v1) or at weaning (v2) against experimental challenge infection. four weeks after vaccina ...	2016	27025924
gastrointestinal degradation of fumonisin b₁ by carboxylesterase fumd prevents fumonisin induced alteration of sphingolipid metabolism in turkey and swine.	the mycotoxin fumonisin b₁ (fb₁) is a frequent contaminant of feed and causes various adverse health effects in domestic animals. hence, effective strategies are needed to prevent the impact of fumonisins on livestock productivity. here we evaluated the capability of the fumonisin carboxylesterase fumd to degrade fb₁ to its less toxic metabolite hydrolyzed fb₁ (hfb₁) in the gastrointestinal tract of turkeys and pigs. first, an ex vivo pig model was used to examine the activity of fumd under dige ...	2016	27007395
updates on feline aelurostrongylosis and research priorities for the next decade.	feline aelurostrongylosis, caused by the metastrongyloid nematode aelurostrongylus abstrusus, is an important gastropod-borne parasitic lung disease in cats. infection with a. abstrusus is widespread globally, but the increasing awareness of this parasite and the advent of more sensitive diagnostics have contributed to the apparent increase in its prevalence and geographic expansion. clinical features may range in severity from subclinical to life-threatening respiratory disease. parasitological ...	2016	27387914
generating chimeric mice by using embryos from nonsuperovulated balb/c mice compared with superovulated balb/c and albino c57bl/6 mice.	the reliable generation of high-percentage chimeras from gene-targeted c57bl/6 embryonic stem cells has proven challenging, despite optimization of cell culture and microinjection techniques. to improve the efficiency of this procedure, we compared the generation of chimeras by using 3 different inbred, albino host, embryo-generating protocols: balb/canntac (balb/c) donor mice superovulated at 4 wk of age, 12-wk-old balb/c donor mice without superovulation, and c57bl/6ntac-tyr(tm1arte) (albino b ...	2016	27423145
simultaneous quantification and differentiation of streptococcus suis serotypes 2 and 9 by quantitative real-time pcr, evaluated in tonsillar and nasal samples of pigs.	invasive streptococcus suis (s. suis) infections in pigs are often associated with serotypes 2 and 9. mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. to unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. a quantitative real-time pcr (qpcr) targeting cps2j (serotypes 2 and 1/2) and cps9h (serotype 9) was evaluated with nasal and tonsillar samples from s. suis expos ...	2016	27376336
purification, crystallization and structural elucidation of d-galactaro-1,4-lactone cycloisomerase from agrobacterium tumefaciens involved in pectin degradation.	pectin is found in the cell wall of plants and is often discarded as waste. a number of research groups are interested in redirecting this biomass waste stream for the production of fuel and bulk chemicals. the primary monomeric subunit of this polysaccharide is d-galacturonate, a six-carbon acid sugar that is degraded in a five-step pathway to central metabolic intermediates by some bacteria, including agrobacterium tumefaciens. in the third step of the pathway, d-galactaro-1,4-lactone is conve ...	2016	26750482
severe community-acquired bloodstream infection with acinetobacter ursingii in person who injects drugs.	we report a community-acquired bloodstream infection with acinteobacter ursingii in an hiv-negative woman who injected drugs. the infection was successfully treated with meropenem. species identification was performed by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. improved identification of acinetobacter spp. by using this method will help identify clinical effects of this underdiagnosed pathogen.	2016	26689082
factors associated with development of canine infectious respiratory disease complex (cirdc) in dogs in 5 canadian small animal clinics.	this study investigated the association between presence of respiratory pathogens and development of canine infectious respiratory disease complex (cirdc) in dogs in 5 canadian small animal clinics. in total, 86 dogs were tested using a commercial pcr respiratory panel; 64 dogs were considered as cases and 22 were control dogs matched by veterinary clinic. no control animals (0/22) were positive for canine parainfluenza virus (cpiv), whereas 27/64 (42%) cirdc cases were positive. furthermore, 81 ...	2016	26740697
monitoring of antimicrobial susceptibility of respiratory tract pathogens isolated from diseased cattle and pigs across europe, 2009-2012: vetpath results.	vetpath is an ongoing pan-european antibiotic susceptibility monitoring programme that collects pathogens from diseased cattle, pigs and poultry. in the current study, 996 isolates from cattle and pig respiratory tract infections were tested for their antimicrobial susceptibilities. non-replicate lung samples or nasopharyngeal/nasal swabs were collected from animals with acute clinical signs in 10 countries during 2009-2012. pasteurella multocida, mannheimia haemolytica and histophilus somni fro ...	2016	27102206
whole genome sequence analysis of pig respiratory bacterial pathogens with elevated minimum inhibitory concentrations for macrolides.	macrolides are often used to treat and control bacterial pathogens causing respiratory disease in pigs. this study analyzed the whole genome sequences of one clinical isolate of actinobacillus pleuropneumoniae, haemophilus parasuis, pasteurella multocida, and bordetella bronchiseptica, all isolated from australian pigs to identify the mechanism underlying the elevated minimum inhibitory concentrations (mics) for erythromycin, tilmicosin, or tulathromycin. the h. parasuis assembled genome had a n ...	2016	26981707
bordetella bronchiseptica antigen enhances the production of mycoplasma hyopneumoniae antigen-specific immunoglobulin g in mice.	we previously demonstrated that bordetella bronchiseptica (b. bronchiseptica) antigen (ag) showed high immunostimulatory effects on mouse bone marrow cells (bms) while mycoplasma hyopneumoniae (m. hyopneumoniae) ag showed low. the focus of this study was if b. bronchiseptica ag may enhance m. hyopneumoniae ag-specific immune response, and also the host immune system can recognize both ags. mtt assay revealed that each or both ags did not significantly change the cell metabolic activity of bms. f ...	2016	27659847
survival of bacteria of laboratory animal origin on cage bedding and inactivation by hydrogen peroxide vapour.	this study aims to determine the ability of laboratory animal bacteria to resist desiccation and inactivation by hydrogen peroxide vapour (hpv) on paper bedding pieces. bedding pieces were saturated with bacterial suspensions in water or 2% (w/v) bovine serum albumin (bsa) in water, and held in a mouse facility. viable counts showed variable survival rates over time for the bacterial species used ([pasteurella] pneumotropica, muribacter muris, pseudomonas aeruginosa, acinetobacter redioresistens ...	2016	27932683
greater efficacy of the ecms-oil adjuvant over other formulations on immune responses against bordetella bronchiseptica in rabbits and the underlying mechanism.	in this study, the adjuvant effects of the extract of cochinchina momordica seed ecms+oil, oil alone, ecms alone, conventional alum adjuvant on inactivated bordetella bronchiseptica (bb) vaccine or control using antigen alone without adjuvant were evaluated along with the underlying mechanism. the results in experiment a demonstrated that antibody levels in bb whole cell protein in the ecms800μg+oil group were significantly higher than in the other adjuvant groups (p<0.05) on day 21. the aggluti ...	2016	27288753
occurence of bordetella bronchiseptica in domestic cats with upper respiratory tract infections.	bordetella bronchiseptica is a widespread gram-negative pathogen occurring in different mammal species. it is known to play a role in the etiology of infectious atrophic rhinitis of swine, canine kennel cough, respiratory syndromes of cats, rabbits and guinea pigs, and sporadic human cases have also been reported. the aim of this article is to present the occurrence of infections caused by these bacteria in domestic cats with respiratory symptoms, as well as to conduct a molecular analysis of th ...	2016	27487509
successful treatment of bordetella bronchiseptica pneumonia by minocycline in anti-neutrophil cytoplasmic antibodies-associated vasculitis patient.	bordetella bronchiseptica is a bacterial pathogen usually isolated from animals and rarely causes human infections. there are, however, some reports that b. bronchiseptica causes human respiratory infections in immunocompromised patients or those with underlying respiratory diseases, although there is a lack of treatment guidelines. an 80-year-old woman was admitted to our hospital to treat anti-neutrophil cytoplasmic antibodies-associated vasculitis. on the 16th day after admission, she complai ...	2016	27424791
comparison of ribotyping and sequence-based typing for discriminating among isolates of bordetella bronchiseptica.	pvuii ribotyping and mlst are each highly discriminatory methods for genotyping bordetella bronchiseptica, but a direct comparison between these approaches has not been undertaken. the goal of this study was to directly compare the discriminatory power of pvuii ribotyping and mlst, using a single set of geographically and genetically diverse strains, and to determine whether subtyping based on repeat region sequences of the pertactin gene (prn) provides additional resolution. one hundred twenty- ...	2016	27542997
molecular surveillance of traditional and emerging pathogens associated with canine infectious respiratory disease.	a molecular survey for traditional and emerging pathogens associated with canine infectious respiratory disease (cird) was conducted in italy between 2011 and 2013 on a total of 138 dogs, including 78 early acute clinically ill cird animals, 22 non-clinical but exposed to clinically ill cird dogs and 38 cird convalescent dogs. the results showed that canine parainfluenza virus (cpiv) was the most commonly detected cird pathogen, followed by canine respiratory coronavirus (crcov), bordetella bron ...	2016	27527760
recent nanotechnology approaches for prevention and treatment of biofilm-associated infections on medical devices.	bacterial colonization in the form of biofilms on surfaces causes persistent infections and is an issue of considerable concern to healthcare providers. there is an urgent need for novel antimicrobial or antibiofilm surfaces and biomedical devices that provide protection against biofilm formation and planktonic pathogens, including antibiotic resistant strains. in this context, recent developments in the material science and engineering fields and steady progress in the nanotechnology field have ...	2016	27872845
pertussis-like syndrome associated with adenovirus presenting with hyperleukocytosis: case report.	adenovirus is an infectious viral agent that causes variety of clinical presentations such as respiratory disease, conjunctivitis, and gastroenteritis. hepatitis, pancreatitis, myocarditis, encephalitis, and disseminated infection are primarily seen in immunocompromised patients. rarely, adenovirus infection can present with pertussis-like syndrome. described here is case of pertussis-like syndrome associated with adenovirus presenting with hyperleukocytosis.	2016	28058402
complete genome sequences of bordetella flabilis, bordetella bronchialis, and "bordetella pseudohinzii".	we report here the complete genome sequences of bordetella flabilis and bordetella bronchialis recovered from cultures of individuals with cystic fibrosis (cf), and "bordetella pseudohinzii" recovered from a cf mouse model.	2016	27738041
differential regulation of type iii secretion and virulence genes in bordetella pertussis and bordetella bronchiseptica by a secreted anti-σ factor.	the bvgas phosphorelay regulates ∼10% of the annotated genomes of bordetella pertussis and bordetella bronchiseptica and controls their infectious cycles. the hierarchical organization of the regulatory network allows the integration of contextual signals to control all or specific subsets of bvgas-regulated genes. here, we characterize a regulatory node involving a type iii secretion system (t3ss)-exported protein, btra, and demonstrate its role in determining fundamental differences in t3ss ph ...	2016	26884180
identification and analysis of the porcine microrna in porcine cytomegalovirus-infected macrophages using deep sequencing.	porcine cytomegalovirus (pcmv; genus cytomegalovirus, subfamily betaherpesvirinae, family herpesviridae) is an immunosuppressive virus that mainly inhibits the immune function of t lymphocytes and macrophages, which has caused substantial damage in the farming industry. in this study, we obtained the mirna expression profiles of pcmv-infected porcine macrophages via high-throughput sequencing. the comprehensive analysis of mirna profiles showed that 239 mirna database-annotated and 355 novel pig ...	2016	26943793
the non-genomic actions of vitamin d.	since its discovery in 1920, a great deal of effort has gone into investigating the physiological actions of vitamin d and the impact its deficiency has on human health. despite this intense interest, there is still disagreement on what constitutes the lower boundary of adequacy and on the recommended dietary allowance. there has also been a major push to elucidate the biochemistry of vitamin d, its metabolic pathways and the mechanisms that mediate its action. originally thought to act by alter ...	2016	26950144
pharmacokinetics of ceftiofur crystalline-free acid in clinically healthy dogs (canis lupus familiaris).	economical, injectable antibiotics are beneficial when clinical manifestations of an animal model prevent the use of oral antibiotics. ceftiofur crystalline-free acid (ccfa) is an injectable, sustained-release form of ceftiofur, a third-generation cephalosporin that is labeled for use in swine, cattle, and horses. because ccfa is an economical, injectable antibiotic that could be of value for use in research dogs, the objective of this study was to determine the pharmacokinetic properties of ccf ...	2016	27025816
comparative genomic analysis of the swine pathogen bordetella bronchisepticastrain km22.	the well-characterized bordetella bronchiseptica strain km22, originally isolated from a pig with atrophic rhinitis, has been used to develop a reproducible swine respiratory disease model. the goal of this study was to identify genetic features unique to km22 by comparing the genome sequence of km22 to the laboratory reference strain rb50. to gain a broader perspective of the genetic relationship of km22 among other b. bronchiseptica strains, selected genes of km22 were then compared to five ot ...	2016	26711033
outer membrane protein ompq of bordetella bronchiseptica is required for mature biofilm formation.	bordetella bronchiseptica, an aerobic gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. most known virulence factors in bordetella species are regulated by the bvgas two-component transduction system. the bvg-activated proteins play a critical role during host infection. ompq is an outer membrane porin protein which is expressed under bvgas control. here, we studied the contribution of ompq to ...	2016	26673448
association between pneumocystis spp. and co-infections with bordetella bronchiseptica, mycoplasma hyopneumoniae and pasteurella multocida in austrian pigs with pneumonia.	in this retrospective study, 218 pig lung tissue samples were analyzed to examine a possible association between pneumocystis spp. using in situ hybridization, bordetella bronchiseptica (b.b.) using immunohistochemistry (ihc), mycoplasma hyopneumoniae (m.h.) by quantitative pcr, and pasteurella multocida (p.m.; ihc). compared to the bacterial agents (b.b., 5%; m.h., 30%; p.m., 23%), pneumocystis occurred with a higher prevalence (51%). co-infections with two or three pathogens were present in 28 ...	2016	26654847
prevalence of bordetella holmesii and bordetella bronchiseptica in respiratory tract samples from belgian patients with pertussis-like symptoms by sensitive culture method and mass spectrometry.	insertion sequences is481 and is1001 are targets for molecular detection of respectively bordetella pertussis and bordetella parapertussis. there is a raising concern about specificity of these targets due to sequence similarity with bordetella holmesii and bordetella bronchiseptica. the likelihood of false (para)pertussis diagnoses should be correlated with the prevalence of these organisms in the respiratory tract (rt). from october 2010 until september 2011, 2,207 rt samples were submitted to ...	2016	24579240
pneumonia caused by bordetella bronchiseptica in two hiv-positive patients.	bordetella bronchiseptica (bb) is a gram-negative coccobacillus responsible for respiratory diseases in dogs, cats and rabbits. reports on its development in humans are rare. however, in immunosuppressed patients, especially in those with the immunodeficiency virus (hiv), bb can cause severe pulmonary infections. we report on two cases of pneumonia caused by bb in hiv-positive male patients in a university hospital.	2016	27191248
functional identification of putrescine c- and n-hydroxylases.	the small polyamine putrescine (1,4-diaminobutane) is ubiquitously and abundantly found in all three domains of life. it is a precursor, through n-aminopropylation or n-aminobutylation, for biosynthesis of the longer polyamines spermidine, sym-homospermidine, spermine, and thermospermine and longer and branched chain polyamines. putrescine is also biochemically modified for purposes of metabolic regulation and catabolism, e.g. n-acetylation and n-glutamylation, and for incorporation into special ...	2016	27541336
cilia-associated bacteria in fatal bordetella bronchiseptica pneumonia of dogs and cats.	bordetella bronchiseptica frequently causes nonfatal tracheobronchitis, but its role in fatal pneumonia is less recognized. our study evaluated histologic identification of cilia-associated bacteria as a method for diagnosis of b. bronchiseptica pneumonia. cases of fatal bronchopneumonia were studied retrospectively, excluding neonates and cases of aspiration pneumonia, minor lung lesions, or autolysis. the study population comprised 36 canine and 31 feline cases of bronchopneumonia. b. bronchis ...	2016	27178716
[bordetella bronchiseptica recurrent bacteraemia in a patient with bone marrow transplantation].	we report a case of recurrent bacteraemia caused by bordetella bronchiseptica in an immunocompromised patient with a history of allogenic bone marrow transplantation for myelodysplastic syndrome, who was admitted to hospital with febrile syndrome. bordetella bronchiseptica is an uncommon human pathogen which mainly affects immunocompromised patients, being a rare cause of bacteraemia.	2016	26849691
the bvg-repressed gene brta, encoding biofilm-associated surface adhesin, is expressed during host infection by bordetella bronchiseptica.	bordetella species display phase modulation between bvg(+) and bvg(-) phases. because expression of known virulence factors is up-regulated in the bvg(+) phase, bacteria in this phase are considered competent for infection. however, the bvg(-) phase is of negligible importance for infection. no studies have shown that bacterial factors specific to the bvg(-) phase (bvg-repressed factors) are expressed in the course of bordetella infection. in the present study, the gene brta (bordetella rtx-fami ...	2016	26756546
antimicrobial susceptibility monitoring of bacterial pathogens isolated from respiratory tract infections in dogs and cats across europe: compath results.	compath is a pan-european resistance monitoring programme collecting bacterial pathogens from dogs and cats. we present data for respiratory tract infection (rti) isolates collected between 2008 and 2010. antimicrobial minimal inhibitory concentrations (mics) were determined and susceptibility calculated following clinical laboratory standards institute (clsi) standards for veterinary medicine. the main pathogen from dogs was staphylococcus intermedius group (49/215, 22.8%) which was >90% suscep ...	2016	27374906
pulmonary cowpox in cats: five cases.	this case series documents five cases of pneumonia (with pleural effusion in three cases) caused by cowpox virus (cpxv) in domestic cats. predisposition to pneumonia may have resulted from mixed infections in two cases (feline herpesvirus and bordetella bronchiseptica in one cat, and mycoplasma species in the other).	2016	25972246
comparative efficacy of intranasal and oral vaccines against bordetella bronchiseptica in dogs.	in order to determine the comparative efficacy of vaccines administered intranasally or orally to protect puppies from disease subsequent to experimental infection with bordetella bronchiseptica (bb), a randomized controlled trial was performed using 48 approximately 8-week-old specific pathogen free, bb naive beagle puppies. puppies were randomized into three groups and administered vaccines containing bb intranasally or orally, or a placebo intranasally. twenty-one days later, all dogs were ch ...	2016	27256028
structure activity characterization of bordetella petrii lipid a, from environment to human isolates.	bordetella petrii, a facultative anaerobic species, is the only known member of the bordetella genus with environmental origin. however it was also recently isolated from humans. the structures of the b. petrii lipid a moieties of the endotoxins were characterized here for the first time for an environmental strain and compared to that of human isolates. characterization was achieved using chemical analyses, gas chromatography-mass spectrometry, and matrix assisted laser desorption ionisation ma ...	2016	26164553
efficacy of intranasal administration of a modified live feline herpesvirus 1 and feline calicivirus vaccine against disease caused by bordetella bronchiseptica after experimental challenge.	studies suggest that intranasal vaccination can stimulate nonspecific immunity against agents not contained within the vaccine, but this effect is not reported for cats.	2016	22860699
molecular detection of infectious pathogens of the upper respiratory tract in captive nondomestic felids.	upper respiratory tract disease is a complex infectious disease process with multiple pathogens involved. identification of infectious agents in wild animals is of great importance for wildlife conservation. the aim of this study was to evaluate the molecular detection of feline herpesvirus type 1, feline calicivirus (fcv), bordetella bronchiseptica , chlamydophila felis , and mycoplasma felis using ocular and nasal swabs in three species of captive nondomestic felids. mycoplasma felis was detec ...	2017	28749292
ancient bacteria-amoeba relationships and pathogenic animal bacteria.	long before bacteria infected humans, they infected amoebas, which remain a potentially important reservoir for human disease. diverse soil amoebas including dictyostelium and acanthamoeba can host intracellular bacteria. though the internal environment of free-living amoebas is similar in many ways to that of mammalian macrophages, they differ in a number of important ways, including temperature. a new study in plos biology by taylor-mulneix et al. demonstrates that bordetella bronchiseptica ha ...	2017	28463965
bordetella bronchiseptica exploits the complex life cycle of dictyostelium discoideum as an amplifying transmission vector.	multiple lines of evidence suggest that bordetella species have a significant life stage outside of the mammalian respiratory tract that has yet to be defined. the bordetella virulence gene (bvgas) two-component system, a paradigm for a global virulence regulon, controls the expression of many "virulence factors" expressed in the bvg positive (bvg+) phase that are necessary for successful respiratory tract infection. a similarly large set of highly conserved genes are expressed under bvg negativ ...	2017	28403138
the bordetella bps polysaccharide is required for biofilm formation and enhances survival in the lower respiratory tract of swine.	bordetella bronchiseptica is pervasive in swine populations and plays multiple roles in respiratory disease. additionally, b. bronchiseptica is capable of establishing long-term or chronic infections in swine. bacterial biofilms are increasingly recognized as important contributors to chronic bacterial infections. recently the polysaccharide locus bpsabcd has been demonstrated to serve a critical role in the development of mature biofilms formed by the sequenced laboratory strain of b. bronchise ...	2017	28559403
bordetella effector bopn is translocated into host cells via its n-terminal residues.	bordetella bronchiseptica infects a wide variety of mammals, the type iii secretion system (t3ss) being involved in long-term colonization by bordetella of the trachea and lung. t3ss translocates virulence factors (commonly referred to as effectors) into host cells, leading to alterations in the host's physiological function. the bordetella effectors bopn and btea are known to have roles in up-regulation of il-10 and cytotoxicity, respectively. nevertheless, the mechanism by which bopn is transl ...	2017	28500733
comparative efficacy of intranasal and injectable vaccines in stimulating bordetella bronchiseptica-reactive anamnestic antibody responses in household dogs.	in order to determine the comparative efficacy of injectable and intranasal vaccines to stimulate bordetella bronchiseptica (bb)-reactive anamnestic antibodies, a trial was conducted using 144 adult household dogs of various breeds and ages, which had been previously administered intranasal bb vaccine approximately 12 months before enrollment. dogs were randomized into 2 groups and blood, nasal swabs, and pharyngeal swabs were collected prior to the administration of single component bb vaccines ...	2017	28761185
correction: btea secreted from the bordetella bronchiseptica type iii secetion system induces necrosis through an actin cytoskeleton signaling pathway and inhibits phagocytosis by macrophages.	[this corrects the article doi: 10.1371/journal.pone.0148387.].	2017	28640910
occurrence of pasteurella multocida among pigs with respiratory disease in china between 2011 and 2015.	prior to the 1990s, p. multocida capsular serogroup a was the most prevalent in china, followed by serogroups b and d. thirty years later, serogroup d became the most prevalent, followed by serogroups a and b. however, the p. multocida capsular serogroups currently circulating in china remain unclear. therefore, the aim of the present study was to provide an update on p. multocida serogroups isolated from diagnostic samples collected from clinically diseased pigs in central and eastern china fro ...	2017	28078081
the extent of the temperature-induced membrane remodeling in two closely related bordetella species reflects their adaptation to diverse environmental niches.	changes in environmental temperature represent one of the major stresses faced by microorganisms as it affects the function of the cytoplasmic membrane. in this study we have analyzed the thermal adaptation in two closely related respiratory pathogens bordetella pertussis and bordetella bronchiseptica. while b. pertussis represents a pathogen strictly adapted to the human body temperature, b. bronchiseptica causes infection in a broad range of animals and survives also outside of the host. we ap ...	2017	28348085
bacterial microbiota in harbor seals (phoca vitulina) from the north sea of schleswig-holstein, germany, around the time of morbillivirus and influenza epidemics.	we present microbiologic findings in harbor seal (phoca; phoca vitulina ) carcasses collected from the north sea of schleswig-holstein, germany, 1996-2014, and interpret results in relation to potential variations caused by phocine distemper virus and influenza a virus mass mortalities. we conducted microbiologic investigations on 2,124 tissue samples from lung, liver, kidney, spleen, intestine, and mesenteric lymph nodes from 549 dead harbor seals of the german north sea. a large variety of bac ...	2017	28139956
modifications of bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity.	bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. although a considerably amount of research has been generated regarding lipopolysaccharide (lps) role during infection and stimulating innate and adaptive immune response, mechanisms involved in lps synthesis are still unknown. in this context we searched in b. bronchiseptica genome for putative glycosyltransferases. we found possible genes codifying for enzymes involved in sugar substitution of the lps ...	2017	28040392
saccharedens versatilis gen. nov., sp. nov., a sugar-degrading member of the burkholderiales isolated from cephalotes rohweri ant guts.	cephalotes 'turtle' ants host a core group of gut-associated symbionts, but their potential contributions to ant nutrition and disease resistance remain uncharacterized in vitro. to gain a better understanding of the metabolic capability of core symbionts belonging to the burkholderiales, we cultivated and characterized strain cag32t from the guts of cephalotes rohweri ants. strain cag32t was rod-shaped, gram-stain-negative, motile and formed pale-white colonies on trypticase soy agar. optimum g ...	2017	27902262
essential role of bordetella nadc in a quinolinate salvage pathway for nad biosynthesis.	nicotinamide adenine dinucleotide (nad) is produced via de novo biosynthesis pathways and by salvage or recycling routes. the classical bordetella bacterial species are known to be auxotrophic for nicotinamide or nicotinic acid. this study confirmed that bordetella bronchiseptica, bordetella pertussis and bordetella parapertussis have the recycling/salvage pathway genes pnca and pncb, for use of nicotinamide or nicotinic acid, respectively, for nad synthesis. although these bordetellae lack the ...	2017	27783449
pasteurella multocida toxin triggers rankl-independent osteoclastogenesis.	bone remodeling is a continuous process to retain the structural integrity and function of the skeleton. a tight coupling is maintained between osteoclast-mediated resorption of old or damaged bones and osteoblast-mediated formation of new bones for bone homeostasis. while osteoblasts differentiate from mesenchymal stem cells, osteoclasts are hematopoietic in origin and derived from myeloid precursor cells. osteoclast differentiation is driven by two cytokines, cytokine receptor activator of nf- ...	2017	28289415
the detection and differentiation of canine respiratory pathogens using oligonucleotide microarrays.	canine respiratory diseases are commonly seen in dogs along with co-infections with multiple respiratory pathogens, including viruses and bacteria. virus infections in even vaccinated dogs were also reported. the clinical signs caused by different respiratory etiological agents are similar, which makes differential diagnosis imperative. an oligonucleotide microarray system was developed in this study. the wild type and vaccine strains of canine distemper virus (cdv), influenza virus, canine herp ...	2017	28189583
environmental origin of the genus bordetella.	members of the genus bordetella include human and animal pathogens that cause a variety of respiratory infections, including whooping cough in humans. despite the long known ability to switch between a within-animal and an extra-host lifestyle under laboratory growth conditions, no extra-host niches of pathogenic bordetella species have been defined. to better understand the distribution of bordetella species in the environment, we probed the ncbi nucleotide database with the 16s ribosomal rna ( ...	2017	28174558
bordetella plrsr regulatory system controls bvgas activity and virulence in the lower respiratory tract.	bacterial pathogens coordinate virulence using two-component regulatory systems (tcs). the bordetella virulence gene (bvgas) phosphorelay-type tcs controls expression of all known protein virulence factor-encoding genes and is considered the "master virulence regulator" in bordetella pertussis, the causal agent of pertussis, and related organisms, including the broad host range pathogen bordetella bronchiseptica we recently discovered an additional sensor kinase, plrs [for persistence in the low ...	2017	28167784
the first siphoviridae family bacteriophages infecting bordetella bronchiseptica isolated from environment.	bordetella bronchiseptica is a well-known etiological agent of kennel cough in dogs and cats and one of the two causative agents of atrophic rhinitis, a serious swine disease. the aim of the study was to isolate b. bronchiseptica bacteriophages from environmental samples for the first time. a total of 29 phages from 65 water samples were isolated using the strain atcc 10580 as a host. the lytic spectra of the phages were examined at 25 and 37 °c, using 12 strains of b. bronchiseptica. all phages ...	2017	27628741
survey of susceptibility to marbofloxacin in bacteria isolated from diseased pigs in europe.	a monitoring programme of marbofloxacin susceptibility of bacteria from europe causing respiratory tract infection and meningitis in pigs has been active since 1994 and 2002, respectively. monitoring digestive, metritis and urinary tract infection (uti) in pigs has been active since 2005 and susceptibility results until 2013 are presented. minimum inhibitory concentration (mic) was determined by broth microdilution. for mic interpretation, vétoquinol-evaluated breakpoints were applied. for diges ...	2017	28348142
abstracts.		2017	28044356
optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus miseq platform.	the composition of the skin microbiome is predicted to play a role in the development of conditions such as atopic eczema and psoriasis. 16s rrna gene sequencing allows the investigation of bacterial microbiota. a significant challenge in this field is development of cost effective high throughput methodologies for the robust interrogation of the skin microbiota, where biomass is low. here we describe validation of methodologies for 16s rrna (ribosomal ribonucleic acid) gene sequencing from the ...	2017	28109256
a conserved tlr5 binding and activation hot spot on flagellin.	flagellin is a bacterial protein that polymerizes into the flagellar filament and is essential for bacterial motility. when flagellated bacteria invade the host, flagellin is recognized by toll-like receptor 5 (tlr5) as a pathogen invasion signal and eventually evokes the innate immune response. here, we provide a conserved structural mechanism by which flagellins from gram-negative γ-proteobacteria and gram-positive firmicutes bacteria bind and activate tlr5. the comparative structural analysis ...	2017	28106112