| development and evaluation of immunochromatographic assay for simple and rapid detection of campylobacter jejuni and campylobacter coli in human stool specimens. | an immunochromatographic assay (campy-ica) using a newly generated single monoclonal antibody against a 15-kda cell surface protein of campylobacter jejuni was developed. when cell suspensions of 86 c. jejuni strains and 27 campylobacter coli strains were treated with a commercially available bacterial protein extraction reagent and the resulting extracts were tested with the campy-ica, they all yielded positive results. the minimum detectable limits for the c. jejuni strains ranged from 1.8 x 1 ... | 2008 | 18256225 |
| detoxification of toxic heavy metals by marine bacteria highly resistant to mercury. | pollution in industrial areas is a serious environmental concern, and interest in bacterial resistance to heavy metals is of practical significance. mercury (hg), cadmium (cd), and lead (pb) are known to cause damage to living organisms, including human beings. several marine bacteria highly resistant to mercury (bhrm) capable of growing at 25 ppm (mg l(-1)) or higher concentrations of mercury were tested during this study to evaluate their potential to detoxify cd and pb. results indicate their ... | 2008 | 18288535 |
| fed batch bioconversion of 2-propanol by a solvent tolerant strain of alcaligenes faecalis entrapped in ca-alginate gel. | a gram-negative, rod-shaped, aerobe, capable of converting 2-propanol (isopropanol, ipa) to acetone was isolated from an oil/sump, and identified by 16 s rdna analysis as alcaligenes faecalis. investigations showed this strain to be extremely solvent-tolerant and it was subsequently named st1. in this study, a. faecalis st1 cells were immobilized by entrapment in ca-alginate beads (3 mm in diameter), and used in the bioconversion of high concentration ipa. the biodegradation rates and the corres ... | 2008 | 18293022 |
| the enzyme mechanism of nitrite reductase studied at single-molecule level. | a generic method is described for the fluorescence "readout" of the activity of single redox enzyme molecules based on förster resonance energy transfer from a fluorescent label to the enzyme cofactor. the method is applied to the study of copper-containing nitrite reductase from alcaligenes faecalis s-6 immobilized on a glass surface. the parameters extracted from the single-molecule fluorescence time traces can be connected to and agree with the macroscopic ensemble averaged kinetic constants. ... | 2008 | 18303118 |
| enzymatic preparation of cefaclor with immobilized penicillin acylase. | enzymatic syntheses of cefaclor by immobilized penicillin acylase under kinetic control were carried out. according to the initial reaction rate ratio of synthesis to hydrolysis (vs/vh), penicillin acylase from alcaligenes faecalis was chosen as the suitable catalyst for the synthesis of cefaclor. the reaction conditions, such as temperature, ph, and substrate concentration were investigated based on their vs/vh values. in the process of preparing cefaclor, in situ product removal (ispr) and acy ... | 2008 | 18320464 |
| survey of bacterial diversity in chronic wounds using pyrosequencing, dgge, and full ribosome shotgun sequencing. | chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. these bacterial populations cooperate to promote their own survival and the chronic nature of the infection. few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. the use of 3 separate16s-based molecular amplifications followed by pyrosequencing, shotgun sanger sequencing, and denatur ... | 2008 | 18325110 |
| conserved active site residues limit inhibition of a copper-containing nitrite reductase by small molecules. | the interaction of copper-containing dissimilatory nitrite reductase from alcaligenes faecalis s-6 ( afnir) with each of five small molecules was studied using crystallography and steady-state kinetics. structural studies revealed that each small molecule interacted with the oxidized catalytic type 2 copper of afnir. three small molecules (formate, acetate and nitrate) mimic the substrate by having at least two oxygen atoms for bidentate coordination to the type 2 copper atom. these three anions ... | 2008 | 18358002 |
| novel aromatic ring-hydroxylating dioxygenase genes from coastal marine sediments of patagonia. | polycyclic aromatic hydrocarbons (pahs), widespread pollutants in the marine environment, can produce adverse effects in marine organisms and can be transferred to humans through seafood. our knowledge of pah-degrading bacterial populations in the marine environment is still very limited, and mainly originates from studies of cultured bacteria. in this work, genes coding catabolic enzymes from pah-biodegradation pathways were characterized in coastal sediments of patagonia with different levels ... | 2008 | 18366740 |
| an amino acid at position 142 in nitrilase from rhodococcus rhodochrous atcc 33278 determines the substrate specificity for aliphatic and aromatic nitriles. | nitrilase from rhodococcus rhodochrous atcc 33278 hydrolyses both aliphatic and aromatic nitriles. replacing tyr-142 in the wild-type enzyme with the aromatic amino acid phenylalanine did not alter specificity for either substrate. however, the mutants containing non-polar aliphatic amino acids (alanine, valine and leucine) at position 142 were specific only for aromatic substrates such as benzonitrile, m-tolunitrile and 2-cyanopyridine, and not for aliphatic substrates. these results suggest th ... | 2008 | 18412544 |
| analysis of bacterial dna in synovial tissue of tunisian patients with reactive and undifferentiated arthritis by broad-range pcr, cloning and sequencing. | bacteria and/or their antigens have been implicated in the pathogenesis of reactive arthritis (rea). several studies have reported the presence of bacterial antigens and nucleic acids of bacteria other than those specified by diagnostic criteria for rea in joint specimens from patients with rea and various arthritides. the present study was conducted to detect any bacterial dna and identify bacterial species that are present in the synovial tissue of tunisian patients with reactive arthritis and ... | 2008 | 18412942 |
| the structures of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase before and after nad+ and acetate binding suggest a dynamical reaction mechanism as a member of the sdr family. | d-3-hydroxybutyrate dehydrogenase, which catalyzes the reversible reaction between d-3-hydroxybutyrate and acetoacetate, has been classified into the short-chain dehydrogenase/reductase family and is a useful marker in the assay of diabetes mellitus and/or ketoacidosis. the enzyme from alcaligenes faecalis was crystallized in the apo form and in the holo form with acetate as a substrate analogue. the crystal structures of both forms were determined at 2.2 angstroms resolution. the enzyme is a te ... | 2008 | 18453685 |
| nitrogen fixation island and rhizosphere competence traits in the genome of root-associated pseudomonas stutzeri a1501. | the capacity to fix nitrogen is widely distributed in phyla of bacteria and archaea but has long been considered to be absent from the pseudomonas genus. we report here the complete genome sequencing of nitrogen-fixing root-associated pseudomonas stutzeri a1501. the genome consists of a single circular chromosome with 4,567,418 bp. comparative genomics revealed that, among 4,146 protein-encoding genes, 1,977 have orthologs in each of the five other pseudomonas representative species sequenced to ... | 2008 | 18495935 |
| anaerobic growth of alcaligenes faecalis var. denitrificans at the expense of ether glycols and nonionic detergents. | | 1983 | 18548680 |
| dectin-1 synergizes with tlr2 and tlr4 for cytokine production in human primary monocytes and macrophages. | the beta-glucan receptor dectin-1 and toll-like receptors tlr2 and tlr4 are the main receptors for recognition of candida albicans by the innate immune system. it has been reported that dectin-1 amplifies tlr2-dependent induction of cytokines in mouse models. in the present study we hypothesized that dectin-1 has potent synergistic effects with both tlr2 and tlr4 in human pbmcs and macrophages. human pbmcs and monocyte-derived macrophages were stimulated with curdlan, a linear beta-1,3-glucan-po ... | 2008 | 18549457 |
| enzymatic properties of immobilized alcaligenes faecalis cells with cell-associated beta-glucosidase activity. | enzymatic properties of alcaligenes faecalis cells immobilized in polyacrylamide were characterized and compared with those reported for the extracted enzyme, and with those measured for free cells. many of the properties reflected those of the extracted enzyme rather than those measured in the free whole cells prior to immobilization, suggesting cell disruption during immobilization. these properties included the ph activity profile, a slightly broader ph stability profile, and the activation e ... | 1984 | 18553373 |
| sequencing and expression of two arsenic resistance operons with different functions in the highly arsenic-resistant strain ochrobactrum tritici scii24t. | arsenic (as) is a natural metalloid, widely used in anthropogenic activities, that can exist in different oxidation states. throughout the world, there are several environments contaminated with high amounts of arsenic where many organisms can survive. the most stable arsenical species are arsenate and arsenite that can be subject to chemically and microbiologically oxidation, reduction and methylation reactions. organisms surviving in arsenic contaminated environments can have a diversity of me ... | 2008 | 18554386 |
| production of bioemulsifier by bacillus subtilis, alcaligenes faecalis and enterobacter species in liquid culture. | three bacterial strains isolated from waste crude oil were selected due to their capacity of growing in the presence of hydrocarbons and production of bioemulsifier. the genetic identification (pcr of the 16s rdna gene using fd1 and rd1 primers) of these strains showed their affiliation to bacillus subtilis, alcaligenes faecalis and enterobacter sp. these strains were able to emulsify n-octane, toluene, xylene, mineral oils and crude oil, look promising for bioremediation application. finally, c ... | 2008 | 18554901 |
| expanded-spectrum beta-lactamase per-1 in an environmental aeromonas media isolate from switzerland. | | 2008 | 18644966 |
| misidentification of pandoraea sputorum isolated from sputum of a patient with cystic fibrosis and review of pandoraea species infections in transplant patients. | pandoraea species are considered emerging pathogens in cystic fibrosis (cf) patients, but few data exist regarding outcomes of patients colonized with these organisms. we report a case of pandoraea sputorum colonization in a cf patient under consideration for lung transplantation and review five cases of lung transplantation involving pandoraea species. | 2008 | 18650348 |
| characterization of the proteins of bordetella isolates from turkeys in the uk by polyacrylamide gel electrophoresis. | the proteins of seven bordetella bronchiseptica-like isolates were examined by polyacrylamide gel electrophoresis (page). they were compared with three similar isolates from overseas, one of which is the type strain of bordetella avium, and with bordetella bronchiseptica nctc 452t and alcaligenes faecalis nctc 415. apart from alcaligenes faecalis, there was strong homology between the isolates suggesting their close relationship, but minor differences were apparent, too. the significance of thes ... | 1992 | 18670923 |
| rapid identification of bordetella avium and related organisms on the basis of their cellular carbohydrate patterns. | whole cell hydrolysates of bordetella avium, b. bronchiseptica, b. pertussis, b. parapertussis, alcaligenes faecalis, a. xylosoxidans ssp. denitrificans, a. xylosoxidans ssp. xylosoxidans, and a. piechaudii were analysed for their cellular carbohydrates by capillary gas-chromatography of per-acetylated aldononitriles and o-methyloximes, respectively. all alcaligenaceae species could be discriminated on the basis of their carbohydrate profiles and a few conventional features. taxometric evaluatio ... | 1991 | 18680060 |
| yeast cell factories for fine chemical and api production. | abstract: this review gives an overview of different yeast strains and enzyme classes involved in yeast whole-cell biotransformations. a focus was put on the synthesis of compounds for fine chemical and api (= active pharmaceutical ingredient) production employing single or only few-step enzymatic reactions. accounting for recent success stories in metabolic engineering, the construction and use of synthetic pathways was also highlighted. examples from academia and industry and advances in the f ... | 2008 | 18684335 |
| structure of diethyl phosphate bound to the binuclear metal center of phosphotriesterase. | the bacterial phosphotriesterase (pte) from pseudomonas diminuta catalyzes the hydrolysis of organophosphate esters at rates close to the diffusion limit. x-ray diffraction studies have shown that a binuclear metal center is positioned in the active site of pte and that this complex is responsible for the activation of the nucleophilic water from solvent. in this paper, the three-dimensional structure of pte was determined in the presence of the hydrolysis product, diethyl phosphate (dep), and a ... | 2008 | 18702530 |
| efficacy of intraoperative vancomycin in irrigating solutions on aqueous contamination during phacoemulsification. | to study the efficacy of adding vancomycin in irrigating solutions, in comparison to topical antibiotic given preoperatively for a day, during phacoemulsification, in reducing the anterior chamber (ac) contamination. | 2008 | 18711269 |
| the characterisation of bordetella/alcaligenes-like organisms and their effects on turkey poults and chicks. | eight isolates of the bordetella or alcaligenes-like organisms associated with turkey rhino-tracheitis were examined. five of these isolates had been recovered from the united kingdom and three were foreign isolates. four of the uk isolates came from flocks with mild respiratory disease. the fifth isolate came from birds with no respiratory signs and this appears to be the first report of the recovery of bordetella/alcaligenes from apparently normal turkeys. the field isolates and type strains a ... | 1986 | 18766500 |
| identification of bordetella avium sp. nov. by the api 20 ne system. | the reactions of 47 bordetella avium strains in the api 20 ne micro-test system were compared to those of the species bordetella bronchiseptica, alcaligenes faecalis and alcaligenes denitrificans. the api 20 ne microtest system allowed separation between b. avium and the other species of gram-negative nonfermentative rods investigated. | 1986 | 18766558 |
| a comparative study of avian bordetella-like strains, bordetella bronchiseptica, alcaligenes faecalis and other related nonfermentable bacteria. | cultural and biochemical characteristics, electrophoretic patterns of soluble proteins, and pathogenicity in turkey poults, of 30 avian gram-negative nonfermentable bacterial strains acquired from various geographical regions, of which 26 had been isolated from turkeys and one each from a chicken, duck, goose and sharp-tailed munia, were compared to those of bordetella bronchiseptica, alcaligenes faecalis, alcaligenes denitrificans, achromobacter xylosoxidans and bordetella parapertussis. with t ... | 1983 | 18766782 |
| experimental studies on turkey coryza. | disease with severe respiratory symptoms, nasal discharge, swelling of sinuses, conjunctivitis with foamy discharge and mortality has been observed in turkey flocks in israel. of the pathogenic agents isolated from field cases, three, paramyxovirus, yucaipa (yucaipa virus), alcaligenes faecalis (a. faecalis) and mycoplasma gallisepticum (mg) were used to infect turkey poults, singly or in combination, in our attempt to reproduce the field disease symptoms. yucaipa virus alone caused mild symptom ... | 1984 | 18766831 |
| body temperature response of turkey poults infected with alcaligenes faecalis. | experiments were conducted to investigate the colonic temperature (tc) response to alcaligenes faecalis infection in turkeys. one-day-old poults were inoculated intranasally with 0.05 ml of a 24 hour brain heart infusion (bhi) broth culture with a bacterial concentration of 1.0 x 10(9) cells/ml. within 2 hours after inoculation tc was depressed significantly in challenged poults. this depression was significant through day 14 in experiment 1 and day 6 in experiment 2. further studies with steril ... | 1984 | 18766888 |
| the effect of combined and separate infection by alcaligenes faecalis and paramyxovirus (yucaipa) on the surface morphology of the trachea in turkey poults. | sixty-seven 1-day-old turkey poults were inoculated in the infra orbital sinus with 3 x 10(8) alcaligenes faecalis bacteria and/or by 10(5.8)eid 50/ ml of yucaipa virus. twenty-five similar birds served as controls. identification of the agents inoculated was made during the development of disease by means of isolation and serological tests. during the development of the disease, the surface morphology of the trachea in the affected animals revealed cellular oedema associated with a mucus coatin ... | 1985 | 18766948 |
| recent clinical overview of renal and perirenal abscesses in 56 consecutive cases. | the aim of this study was to examine the recent clinical trends and antibiotic susceptibilities of the causative microorganisms in renal and perirenal abscesses, and to elucidate the factors associated with treatment strategies. | 2008 | 18787367 |
| effective mutagenesis of vibrio fischeri by using hyperactive mini-tn5 derivatives. | we have developed a transposon mutagenesis system for vibrio fischeri es114 that utilizes a hyperactive mutant tn5 transposase (e54k and m56a) and optimized transposon ends. using a conjugation-based procedure, we obtained independent single-insertion mini-tn5 mutants at a rate of approximately 10(-6). this simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of v. fischeri and should be applicable to many other bacteria. | 2008 | 18805998 |
| [enhanced production of curdlan by alcaligenes faecalis by selective feeding with ammonia water during the cell growth phase of fermentation]. | curdlan is a water insoluble exopolysaccharide produced by alcaligenes faecalis under nitrogen-limiting conditions. after excretion, the polysaccharide is attached the cell wall. thus enhancement of biomass production during the cell growth phase is important to curdlan production. a strategy of increasing nitrogen source to improve biomass production was adopted for curdlan production by alcaligenes faecalis (atcc 31749). in the batch fermentation of curdlan, a relatively higher nh4cl level of ... | 2008 | 18807988 |
| the missing link: bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic bordetellae. | bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus bordetella. phylogenetically, it connects the pathogenic bordetellae and environmental bacteria of the genera achromobacter and alcaligenes, which are opportunistic pathogens. b. petrii strains have been isolated from very different environmental niches, including river sediment, polluted soil, marine sponges and a grass root. recently, clinical isolates asso ... | 2008 | 18826580 |
| structure, membrane orientation, mechanism, and function of pexiganan--a highly potent antimicrobial peptide designed from magainin. | the growing problem of bacterial resistance to conventional antibiotic compounds and the need for new antibiotics have stimulated interest in the development of antimicrobial peptides (amps) as human therapeutics. development of topically applied agents, such as pexiganan (also known as msi-78, an analog of the naturally occurring magainin2, extracted from the skin of the african frog xenopus laevis) has been the focus of pharmaceutical development largely because of the relative safety of topic ... | 2009 | 19010301 |
| structure, membrane orientation, mechanism, and function of pexiganan--a highly potent antimicrobial peptide designed from magainin. | the growing problem of bacterial resistance to conventional antibiotic compounds and the need for new antibiotics have stimulated interest in the development of antimicrobial peptides (amps) as human therapeutics. development of topically applied agents, such as pexiganan (also known as msi-78, an analog of the naturally occurring magainin2, extracted from the skin of the african frog xenopus laevis) has been the focus of pharmaceutical development largely because of the relative safety of topic ... | 2009 | 19010301 |
| bacterial contamination in drinking water: a case study in rural areas of northern rajasthan, india. | the objective of this study was to determine the bacterial contaminations in drinking water samples collected form some rural habitations of northern rajasthan, india. a total of ten bacterial species: escherichia coli, pseudomonas aeruginosa, enterobacter aerogenes, klebsiella sp, proteus vulgaris, alcaligenes faecalis, bacillus cereus, staphylococcus aureus, streptococcus lactis and micrococcus luteum were identified form drinking water samples. the bacteria belonging to the family enterobacte ... | 2009 | 19023670 |
| impact of enhanced infection control at 2 neonatal intensive care units in the philippines. | the growing burden of neonatal mortality associated with hospital-acquired neonatal sepsis in the developing world creates an urgent need for cost-effective infection-control measures in resource-limited settings. | 2009 | 19025496 |
| evaluation of an immunochromatographic assay kit for rapid identification of mycobacterium tuberculosis complex in clinical isolates. | we evaluated a new immunochromatographic assay (ica) using mouse monoclonal anti-mpt64 antibody for rapid discrimination between mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. a study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) cfu/ml) when tested with m. tuberculosis h37rv. this ica can simplify the identification of m. tuberculosis in clinical labo ... | 2009 | 19052177 |
| evaluation of an immunochromatographic assay kit for rapid identification of mycobacterium tuberculosis complex in clinical isolates. | we evaluated a new immunochromatographic assay (ica) using mouse monoclonal anti-mpt64 antibody for rapid discrimination between mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. a study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) cfu/ml) when tested with m. tuberculosis h37rv. this ica can simplify the identification of m. tuberculosis in clinical labo ... | 2009 | 19052177 |
| dna aptamers bind specifically and selectively to (1-->3)-beta-d-glucans. | (1-->3)-beta-d-glucans are structural cell wall components of fungi, plants, and some bacteria and have been linked with human respiratory symptoms following aerosol exposure. a clear interpretation of the health impact of (1-->3)-beta-d-glucans is limited by the high cost and uncertainties associated with current glucan quantitation methods. the objective of this research is to develop dna aptamers for the measurement of (1-->3)-beta-d-glucans. aptamers are synthetic dna functional binding mole ... | 2009 | 19061867 |
| dna aptamers bind specifically and selectively to (1-->3)-beta-d-glucans. | (1-->3)-beta-d-glucans are structural cell wall components of fungi, plants, and some bacteria and have been linked with human respiratory symptoms following aerosol exposure. a clear interpretation of the health impact of (1-->3)-beta-d-glucans is limited by the high cost and uncertainties associated with current glucan quantitation methods. the objective of this research is to develop dna aptamers for the measurement of (1-->3)-beta-d-glucans. aptamers are synthetic dna functional binding mole ... | 2009 | 19061867 |
| conformational exchange in pseudoazurin: different kinds of microsecond to millisecond dynamics characterized by their ph and buffer dependence using 15n nmr relaxation. | the dynamics of the reduced form of the blue copper protein pseudoazurin from alcaligenes faecalis s-6 was investigated using (15)n relaxation measurements with a focus on the dynamics of the micro- to millisecond time scale. different types of conformational exchange processes are observed in the protein on this time scale. at low ph, the protonation of the c-terminal copper-ligated histidine, his81, is observed. a comparison of the exchange rates in the presence and absence of added buffers sh ... | 2009 | 19072172 |
| genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils. | arsenic is known as a toxic metalloid, which primarily exists in inorganic form [as(iii) and as(v)] and can be transformed by microbial redox processes in the natural environment. as(iii) is much more toxic and mobile than as(v), hence microbial arsenic redox transformation has a major impact on arsenic toxicity and mobility which can greatly influence the human health. our main purpose was to investigate the distribution and diversity of microbial arsenite-resistant species in three different a ... | 2009 | 19128515 |
| internalization of dectin-1 terminates induction of inflammatory responses. | dectin-1 is a pattern-recognition receptor recognizing beta-(1,3)-glucans found on fungal cell walls. dectin-1 plays an important role in immunity to fungi by mediating phagocytic clearance of fungal particles and inducing transcription of innate response genes. we show here that the two processes are linked and that dectin-1 signalling for inflammation is attenuated by phagocytosis. blocking dectin-1 ligand-dependent internalization using either actin polymerization or dynamin inhibitors, large ... | 2009 | 19130473 |
| cd40-cd40l cross-talk integrates strong antigenic signals and microbial stimuli to induce development of il-17-producing cd4+ t cells. | il-17-producing cd4(+) t cells have been recognized as key players in organ-related autoimmune disease; however, the parameters that govern their development are yet to be elucidated fully. by using both in vivo and in vitro systems, we have investigated the role of antigen dose, pathogen-associated molecular patterns, and cd40-cd40 ligand (cd40l) cross-talk in th17 differentiation. we found that the strength of antigenic stimulation critically influenced the extent of th17 differentiation, beca ... | 2009 | 19136631 |
| optgraft: a computational procedure for transferring a binding site onto an existing protein scaffold. | one of the many challenging tasks of protein design is the introduction of a completely new function into an existing protein scaffold. in this study, we introduce a new computational procedure optgraft for placing a novel binding pocket onto a protein structure so as its geometry is minimally perturbed. this is accomplished by introducing a two-level procedure where we first identify where are the most appropriate locations to graft the new binding pocket into the protein fold by minimizing the ... | 2009 | 19177362 |
| optgraft: a computational procedure for transferring a binding site onto an existing protein scaffold. | one of the many challenging tasks of protein design is the introduction of a completely new function into an existing protein scaffold. in this study, we introduce a new computational procedure optgraft for placing a novel binding pocket onto a protein structure so as its geometry is minimally perturbed. this is accomplished by introducing a two-level procedure where we first identify where are the most appropriate locations to graft the new binding pocket into the protein fold by minimizing the ... | 2009 | 19177362 |
| atrazine degradation by stable mixed cultures enriched from agricultural soil and their characterization. | the aim of this work was to enrich stable mixed cultures from atrazine-contaminated soil. the cultures were examined for their atrazine biodegradation efficiencies in comparison with j14a, a known atrazine-degrading strain of agrobacterium radiobacter. the cultures were also characterized to identify community structure and bacterial species present. | 2009 | 19191954 |
| crystallization and preliminary x-ray diffraction analysis of a complex between the electron-transfer partners hexameric cu-containing nitrite reductase and pseudoazurin. | the complex between cu-containing nitrite reductase (hdnir) and its electron-donor protein pseudoazurin (hdpaz) from hyphomicrobium denitrificans has been crystallized. the crystals were obtained from a mixture of the two proteins using the hanging-drop vapour-diffusion method in the presence of polyethylene glycol (peg) and 2-methyl-2,4-pentanediol (mpd) as precipitants. sds-page analysis demonstrated that the crystals contained both proteins. the x-ray diffraction experiment was carried out at ... | 2009 | 19193999 |
| purification and characterization of arsenite oxidase from arthrobacter sp. | the chemolithoautotroph, arthrobacter sp.15b oxidizes arsenite to arsenate using a membrane bound arsenite oxidase. the enzyme arsenite oxidase is purified to its homogeneity and identified using maldi-tof ms analysis. upon further characterization, it was observed that the enzyme is a heterodimer showing native molecular mass as approximately 100 kda and appeared as two subunits of approximately 85 kda lsu and 14 kda ssu on sds-page. the v(max) and k(m) values of the enzyme was found to be 2.45 ... | 2009 | 19214757 |
| crystal structure of a two-domain multicopper oxidase: implications for the evolution of multicopper blue proteins. | the two-domain multicopper oxidases are proposed to be key intermediates in the evolution of three-domain multicopper oxidases. a number of two-domain multicopper oxidases have been identified from genome sequences and are classified as type a, type b, or type c on the basis of the predicted location of the type 1 copper center. the crystal structure of blue copper oxidase, a type c two-domain multicopper oxidase from nitrosomonas europaea, has been determined to 1.9 a resolution. blue copper ox ... | 2009 | 19224923 |
| characterization of a polycyclic aromatic hydrocarbon degradation gene cluster in a phenanthrene-degrading acidovorax strain. | acidovorax sp. strain na3 was isolated from polycyclic aromatic hydrocarbon (pah)-contaminated soil that had been treated in a bioreactor and enriched with phenanthrene. the 16s rrna gene of the isolate possessed 99.8 to 99.9% similarity to the dominant sequences recovered during a previous stable-isotope probing experiment with [u-(13)c]phenanthrene on the same soil (d. r. singleton, s. n. powell, r. sangaiah, a. gold, l. m. ball, and m. d. aitken, appl. environ. microbiol. 71:1202-1209, 2005). ... | 2009 | 19270134 |
| a tn5051-like mer-containing transposon identified in a heavy metal tolerant strain achromobacter sp. ao22. | achromobacter sp. ao22 (formerly alcaligenes sp. ao22), a bacterial strain isolated from a lead-contaminated industrial site in australia, was previously found to be resistant to moderate to high levels of mercury, copper and other heavy metals. however, the nature and location of the genetic basis for mercuric ion resistance in this strain, had not been previously identified. | 2009 | 19284535 |
| arsenite oxidation by immobilized cells of alcaligenes faecalis strain o1201 in a fluidized-bed reactor. | arsenic(iii) oxidation was evaluated in a continuous-flow fluidized-bed reactor (fbr) with alcaligenes faecalis strain 01201 immobilized in gel beads. the fbr was operated under 300 mg/l citrate and a range of influent as(iii) concentrations (75 to 3000 mg/l) at short hydraulic retention times (1.06 to 3.17 hours). the ph and temperature in the fbr were maintained at optimal growth conditions for strain o1201 (ph 7 and 30 degrees c) throughout the study. a total of 10 quasi-steady-state operatin ... | 2009 | 19323288 |
| widespread distribution of cell defense against d-aminoacyl-trnas. | several l-aminoacyl-trna synthetases can transfer a d-amino acid onto their cognate trna(s). this harmful reaction is counteracted by the enzyme d-aminoacyl-trna deacylase. two distinct deacylases were already identified in bacteria (dtd1) and in archaea (dtd2), respectively. evidence was given that dtd1 homologs also exist in nearly all eukaryotes, whereas dtd2 homologs occur in plants. on the other hand, several bacteria, including most cyanobacteria, lack genes encoding a dtd1 homolog. here w ... | 2009 | 19332551 |
| structure of d-3-hydroxybutyrate dehydrogenase prepared in the presence of the substrate d-3-hydroxybutyrate and nad+. | d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis catalyzes the reversible conversion between d-3-hydroxybutyrate and acetoacetate. the enzyme was crystallized in the presence of the substrate d-3-hydroxybutyrate and the cofactor nad(+) at the optimum ph for the catalytic reaction. the structure, which was solved by x-ray crystallography, is isomorphous to that of the complex with the substrate analogue acetate. the product as well as the substrate molecule are accommodated well in the ... | 2009 | 19342772 |
| [denitrifying bacteria isolated from river sediment and its nitrogen and phosphorus removal capacity from river water]. | 20 strains of bacteria were isolated from river sediment using enrichment culture medium for denitrification, and the denitrification intensity was determined. f10, one of bacteria strain, was identified having the highest denitrifying intensity, and further used to test its role in the removing of nitrogen and phosphorus from wastewater in laboratory. by checking the individual morphology, colony culture characteristics, dna sequencing and 16s rdna gene bank, f10 was identified as alcaligenes f ... | 2009 | 19353863 |
| pseudomonas aeruginosa las quorum sensing autoinducer suppresses growth and biofilm production in legionella species. | bacteria commonly communicate with each other by a cell-to-cell signalling mechanism known as quorum sensing (qs). recent studies have shown that the las qs autoinducer n-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-c(12)-hsl) of pseudomonas aeruginosa performs a variety of functions not only in intraspecies communication, but also in interspecies and interkingdom interactions. in this study, we report the effects of pseudomonas 3-oxo-c(12)-hsl on the growth and suppression of virulence factors ... | 2009 | 19383702 |
| mammalian nitrilase 1 homologue nit1 is a negative regulator in t cells. | the mammalian nit1 protein is homologous to plant and bacterial nitrilases. in flies and worms, nit1 is fused to the 5' end of fhit, suggesting that nit1 may functionally interact with the fhit pathway. fhit has been shown to play a role of a tumor suppressor. somatic loss of fhit in human tissues is associated with a wide variety of cancers. deletion of fhit results in a predisposition to induced and spontaneous tumors in mice. it has been suggested that nit1 collaborates with fhit in tumor sup ... | 2009 | 19395373 |
| conjugative type 4 secretion system of a novel large plasmid from the chemoautotroph tetrathiobacter kashmirensis and construction of shuttle vectors for alcaligenaceae. | tetrathiobacter spp. and other members of the alcaligenaceae are metabolically versatile and environmentally significant. a novel, approximately 60-kb conjugative plasmid, pbtk445, from the sulfur chemolithoautotroph tetrathiobacter kashmirensis, was identified and characterized. this plasmid exists at a low copy number of 2 to 3 per host chromosome. the portion of pbtk445 sequenced so far ( approximately 25 kb) harbors genes putatively involved in replication, transfer functions, partition, and ... | 2009 | 19411426 |
| strain-specific virulence of bordetella hinzii in poultry. | bordetella hinzii is commonly acquired from the respiratory tract of diseased poultry but is generally regarded as nonpathogenic in avian hosts because attempts to demonstrate disease following experimental infection of chickens and turkeys have failed. recently, with the availability of highly specific dna-based methods for identification of this agent, it was recognized that some isolates used in previous studies were misidentified at the time of their acquisition as bordetella avium, b. avium ... | 2009 | 19432003 |
| high frequency of multidrug-resistant gram-negative rods in 2 neonatal intensive care units in the philippines. | although hospital-acquired infections appear to be a growing threat to the survival of newborns in the developing world, the epidemiology of this problem remains poorly characterized. | 2009 | 19435448 |
| microflora of the penile skin-lined neovagina of transsexual women. | the microflora of the penile skin-lined neovagina in male-to-female transsexuals is a recently created microbial niche which thus far has been characterized only to a very limited extent. yet the knowledge of this microflora can be considered as essential to the follow-up of transsexual women. the primary objective of this study was to map the neo-vaginal microflora in a group of 50 transsexual women for whom a neovagina was constructed by means of the inverted penile skin flap technique. second ... | 2009 | 19457233 |
| impact of antifungals producing rhizobacteria on the performance of vigna radiata in the presence of arbuscular mycorrhizal fungi. | plant growth-promoting rhizobacteria (pgpr) that produce antifungal metabolites are potential threats for the arbuscular mycorrhizal (am) fungi known for their beneficial symbiosis with plants that is crucially important for low-input sustainable agriculture. to address this issue, we used a compartmented container system where test plants, vigna radiata, could only reach a separate nutrient-rich compartment indirectly via the hyphae of am fungi associated with their roots. in this system, where ... | 2009 | 19458967 |
| functions of manduca sexta hemolymph proteinases hp6 and hp8 in two innate immune pathways. | serine proteinases in insect plasma have been implicated in two types of immune responses; that is, activation of prophenoloxidase (propo) and activation of cytokine-like proteins. we have identified more than 20 serine proteinases in hemolymph of the tobacco hornworm, manduca sexta, but functions are known for only a few of them. we report here functions of two additional m. sexta proteinases, hemolymph proteinases 6 and 8 (hp6 and hp8). hp6 and hp8 are each composed of an amino-terminal clip d ... | 2009 | 19487692 |
| [evaluation of occurrence of alcaligenes faecalis in clinical samples of patients of the university hospital in bydgoszcz]. | alcaligenes faecalis is an aerobic gram-negative, non-fermentative rod. it's saprophyte of water and soil. it may be recovered from wet places of hospital environment. it is considered as an opportunistic pathogen. the aim of this review was evaluation of occurrence in clinical samples and susceptibility to antibiotics of 72 a. faecalis strains isolated in years 2003-2008. over 30% of strains were isolated from patients in surgical ward, 19.6% from patients in outpatient clinic and almost 14% fr ... | 2009 | 19517818 |
| annotating enzymes of uncertain function: the deacylation of d-amino acids by members of the amidohydrolase superfamily. | the catalytic activities of three members of the amidohydrolase superfamily were discovered using amino acid substrate libraries. bb3285 from bordetella bronchiseptica, gox1177 from gluconobacter oxidans, and sco4986 from streptomyces coelicolor are currently annotated as d-aminoacylases or n-acetyl-d-glutamate deacetylases. these three enzymes are 22-34% identical to one another in amino acid sequence. substrate libraries containing nearly all combinations of n-formyl-d-xaa, n-acetyl-d-xaa, n-s ... | 2009 | 19518059 |
| identification of an aox system that requires cytochrome c in the highly arsenic-resistant bacterium ochrobactrum tritici scii24. | microbial biotransformations have a major impact on environments contaminated with toxic elements, including arsenic, resulting in an increasing interest in strategies responsible for how bacteria cope with arsenic. in the present work, we investigated the metabolism of this metalloid in the bacterium ochrobactrum tritici scii24. this heterotrophic organism contains two different ars operons and is able to oxidize arsenite to arsenate. the presence of arsenite oxidase genes in this organism was ... | 2009 | 19525272 |
| novel denitrifying bacterium ochrobactrum anthropi yd50.2 tolerates high levels of reactive nitrogen oxides. | most studies of bacterial denitrification have used nitrate (no3-) as the first electron acceptor, whereas relatively less is understood about nitrite (no2-) denitrification. we isolated novel bacteria that proliferated in the presence of high levels of no2- (72 mm). strain yd50.2, among several isolates, was taxonomically positioned within the alpha subclass of proteobacteria and identified as ochrobactrum anthropi yd50.2. this strain denitrified no2-, as well as no3-. the gene clusters for den ... | 2009 | 19542343 |
| broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis. | broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples. | 2009 | 19570210 |
| identification of amino acid residues responsible for the enantioselectivity and amide formation capacity of the arylacetonitrilase from pseudomonas fluorescens ebc191. | the nitrilase from pseudomonas fluorescens ebc191 converted (r,s)-mandelonitrile with a low enantioselectivity to (r)-mandelic acid and (s)-mandeloamide in a ratio of about 4:1. in contrast, the same substrate was hydrolyzed by the homologous nitrilase from alcaligenes faecalis atcc 8750 almost exclusively to (r)-mandelic acid. a chimeric enzyme between both nitrilases was constructed, which represented in total 16 amino acid exchanges in the central part of the nitrilase from p. fluorescens ebc ... | 2009 | 19581475 |
| demonstration of proton-coupled electron transfer in the copper-containing nitrite reductases. | the reduction of nitrite (no2-) into nitric oxide (no), catalyzed by nitrite reductase, is an important reaction in the denitrification pathway. in this study, the catalytic mechanism of the copper-containing nitrite reductase from alcaligenes xylosoxidans (axnir) has been studied using single and multiple turnover experiments at ph 7.0 and is shown to involve two protons. a novel steady-state assay was developed, in which deoxyhemoglobin was employed as an no scavenger. a moderate solvent kinet ... | 2009 | 19586913 |
| airway infection with a novel cupriavidus species in persons with cystic fibrosis. | we describe the recovery and identification of a bacterium that represents a new species of the genus cupriavidus from cultures of respiratory tract specimens from two patients with cystic fibrosis (cf). the elucidation of the role of this species in cf lung disease will require an evaluation of a greater number of cases. | 2009 | 19605576 |
| synergy between ficolin-2 and pentraxin 3 boosts innate immune recognition and complement deposition. | the long pentraxin 3 (ptx3) is a multifunctional soluble pattern recognition molecule that is crucial in innate immune protection against opportunistic fungal pathogens such as aspergillus fumigatus. the mechanisms that mediate downstream effects of ptx3 are largely unknown. however, ptx3 interacts with c1q from the classical pathway of the complement. the ficolins are recognition molecules of the lectin complement pathway sharing structural and functional characteristics with c1q. thus, we inve ... | 2009 | 19632990 |
| rapid real-time pcr assays for detection of klebsiella pneumoniae with the rmpa or maga genes associated with the hypermucoviscosity phenotype: screening of nonhuman primates. | the relationship of mucoviscosity-associated (maga) and/or regulator of mucoid phenotype (rmpa) genes to the klebsiella pneumoniae hypermucoviscosity (hmv) phenotype has been reported. we previously demonstrated that rmpa+ k. pneumoniae can cause serious disease in african green monkeys and isolated rmpa+ and maga+ hmv k. pneumoniae from other species of non-human primates. to rapidly screen african green monkeys/non-human primates for these infections, we developed three real-time pcr assays. t ... | 2009 | 19644019 |
| characterization of sucrose-glutamic acid maillard products (sgmps) degrading bacteria and their metabolites. | two aerobic bacteria rnbs1 and rnbs3 capable to degrade and utilize sucrose-glutamic acid maillard products (sgmps) as carbon, nitrogen and energy source were isolated and characterized as alcaligenes faecalis (dq659619) and bacillus cereus (dq659620) respectively by 16s rrna gene sequence analysis. in present study, mixed bacterial culture was found more effective compared to axenic culture rnbs1 and rnbs3 decolourizing 73.79%, 66.80% and 62.56% sgmps, respectively. the sgmps catabolizing enzym ... | 2009 | 19665891 |
| paenalcaligenes hominis gen. nov., sp. nov., a new member of the family alcaligenaceae. | a beige-pigmented bacterium (strain ccug 53761a(t)) was isolated from human blood from an 85-year-old man in göteborg, sweden. comparative analysis of 16s rrna gene sequences showed that this bacterium displayed <95 % similarity to all described species of the genera of the family alcaligenaceae. it grouped within the radiation of the genus alcaligenes, but showed only 93.0-94.8 % similarity to type strains of members of this genus (alcaligenes faecalis subsp. parafaecalis, 94.8 %; alcaligenes f ... | 2010 | 19684310 |
| molecular and kinetic characterization of mixed cultures degrading natural and synthetic estrogens. | the biodegradation of estradiol (e2), estrone (e1), and ethinylestradiol (ee2) was investigated using mixed bacterial cultures enriched from activated sludge. enrichments were carried out on e2 or ee2 in batch conditions with acetonitrile as additional carbon source. degradation experiments were performed both using hormones as sole carbon source or with an additional source. the hormones were completely degraded by these cultures. estradiol was rapidly converted to e1 within 24 h. thereafter, e ... | 2010 | 19685239 |
| nitrite reductase genes in halobenzoate degrading denitrifying bacteria. | abstract diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. nitrite reductase genes were pcr-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirs and nirk), cloned and sequenced to determine which type of nitrite reductase was present in each isolate and consortium. halobe ... | 2003 | 19719666 |
| biochemical and genomic analysis of the denitrification pathway within the genus neisseria. | since neisseria gonorrhoeae and neisseria meningitidis are obligate human pathogens, a comparison with commensal species of the same genus could reveal differences important in pathogenesis. the recent completion of commensal neisseria genome draft assemblies allowed us to perform a comparison of the genes involved in the catalysis, assembly and regulation of the denitrification pathway, which has been implicated in the virulence of several bacteria. all species contained a highly conserved nitr ... | 2009 | 19762442 |
| the susceptibility of nosocomial pathogens to ceftazidime. | the activities in vitro of ceftazidime and of cefuroxime have been compared against 201 strains of bacteria which had been isolated either from outbreaks of nosocomial infection or were typical of strains causing such outbreaks. ceftazidime was found to be about 1000 times more active against pseudomonas spp. and proteus vulgaris, and between 10 and 100 times more active against klebsiella pneumoniae, enterobacter spp., citrobacter freundii, serratia marcescens, proteus rettgeri, proteus morgani ... | 1981 | 19802966 |
| 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine, an effective peptide antibiotic from the epiphyte pantoea agglomerans 48b/90. | the epiphyte pantoea agglomerans 48b/90, which has been isolated from soybean leaves, belongs to the enterobacteriaceae, as does the plant pathogen erwinia amylovora, which causes fire blight on rosaceous plants such as apples and leads to severe economic losses. since p. agglomerans efficiently antagonizes phytopathogenic bacteria, the p. agglomerans strain c9-1 is used as a biocontrol agent (blightban c9-1). here we describe the bioassay-guided isolation of a peptide antibiotic that is highly ... | 2009 | 19820144 |
| variation of neisseria gonorrhoeae lipooligosaccharide directs dendritic cell-induced t helper responses. | gonorrhea is one of the most prevalent sexually transmitted diseases in the world. a naturally occurring variation of the terminal carbohydrates on the lipooligosaccharide (los) molecule correlates with altered disease states. here, we investigated the interaction of different stable gonoccocal los phenotypes with human dendritic cells and demonstrate that each variant targets a different set of receptors on the dendritic cell, including the c-type lectins mgl and dc-sign. neisseria gonorrhoeae ... | 2009 | 19834553 |
| immobilization of alcaligenes faecalis penicillin g acylase on epoxy-type supports. | alcaligenes faecalis penicillin g acylase has several desired features over other penicillin g acylases and its use in industry requires immobilization. in this work, two novel supports zh-ep (epoxy-type) and zh-ha (epoxy-amino type) were used to immobilize alcaligenes faecalis penicillin g acylase (afpga) with eupergit c as reference. the saturation of immobilized protein on zh-ep (269 mg/g, 116 h) and zh-ha (296 mg/g, 15 h) was obtained more rapidly than eupergit c (197 mg/g, 260 h). and the a ... | 2009 | 19845283 |
| comparative impact of cadmium on two phenanthrene-degrading bacteria isolated from cadmium and phenanthrene co-contaminated soil in china. | alcaligenes faecalis strain j08 and brevundimonas sp. strain x08 were isolated from soils co-contaminated by cadmium (cd) and polycyclic aromatic hydrocarbons (pahs) in northeast china. the two strains of bacteria were identified by phenotypic tests and 16s rdna. different cd treatments (0.01 mm, 0.1mm, 0.5mm) showed no significant influence (p>0.05) on the biodegradation of phenanthrene by a. faecalis strain j08. brevundimonas sp. strain x08 also presented no significant differences in the biod ... | 2010 | 19853994 |
| assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance. | to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health. | 2009 | 19880150 |
| molecular epidemiology of metallo-beta-lactamase-producing pseudomonas aeruginosa isolates from norway and sweden shows import of international clones and local clonal expansion. | scandinavia is considered a region with a low prevalence of antimicrobial resistance. however, the number of multidrug-resistant (mdr) gram-negative bacteria is increasing, including metallo-beta-lactamase (mbl)-producing pseudomonas aeruginosa. in this study mbl-producing p. aeruginosa isolates identified in norway (n = 4) and sweden (n = 9) from 1999 to 2007 were characterized. two international clonal complexes (cc), cc111 (n = 8) and cc235 (n = 2), previously associated with mbl-producing is ... | 2010 | 19884381 |
| molecular epidemiology of metallo-beta-lactamase-producing pseudomonas aeruginosa isolates from norway and sweden shows import of international clones and local clonal expansion. | scandinavia is considered a region with a low prevalence of antimicrobial resistance. however, the number of multidrug-resistant (mdr) gram-negative bacteria is increasing, including metallo-beta-lactamase (mbl)-producing pseudomonas aeruginosa. in this study mbl-producing p. aeruginosa isolates identified in norway (n = 4) and sweden (n = 9) from 1999 to 2007 were characterized. two international clonal complexes (cc), cc111 (n = 8) and cc235 (n = 2), previously associated with mbl-producing is ... | 2010 | 19884381 |
| outbreak of alcaligenes faecalis pseudobacteraemia in neonatology and paediatric units. | | 2010 | 19900735 |
| got black swimming dots in your cell culture? identification of achromobacter as a novel cell culture contaminant. | cell culture model systems are utilized for their ease of use, relative inexpensiveness, and potentially limitless sample size. reliable results cannot be obtained, however, when cultures contain contamination. this report discusses the observation and identification of mobile black specks observed in multiple cell lines. cultures of the contamination were grown, and dna was purified from isolated colonies. the 16s rdna gene was pcr amplified using primers that will amplify the gene from many ge ... | 2010 | 19926304 |
| got black swimming dots in your cell culture? identification of achromobacter as a novel cell culture contaminant. | cell culture model systems are utilized for their ease of use, relative inexpensiveness, and potentially limitless sample size. reliable results cannot be obtained, however, when cultures contain contamination. this report discusses the observation and identification of mobile black specks observed in multiple cell lines. cultures of the contamination were grown, and dna was purified from isolated colonies. the 16s rdna gene was pcr amplified using primers that will amplify the gene from many ge ... | 2010 | 19926304 |
| international spread and persistence of tem-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an inca/c2 plasmid containing tn1696::tn1 and is5075-tn21. | tem-24 remains one of the most widespread tem-type extended-spectrum beta-lactamases (esbls) among enterobacteriaceae. to analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative tem-24 producers from belgium, france, portugal, and spain (13 enterobacter aerogenes isolates, 6 escherichia coli isolates, 6 klebsiella pneumoniae isolates, 2 proteus mirabilis isolates, and 1 klebsiella oxytoca isolate, from 1998 to 2004). ... | 2010 | 19995930 |
| international spread and persistence of tem-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an inca/c2 plasmid containing tn1696::tn1 and is5075-tn21. | tem-24 remains one of the most widespread tem-type extended-spectrum beta-lactamases (esbls) among enterobacteriaceae. to analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative tem-24 producers from belgium, france, portugal, and spain (13 enterobacter aerogenes isolates, 6 escherichia coli isolates, 6 klebsiella pneumoniae isolates, 2 proteus mirabilis isolates, and 1 klebsiella oxytoca isolate, from 1998 to 2004). ... | 2010 | 19995930 |
| protonation of type-1 cu bound histidines: a quantum chemical study. | the protonation of the solvent-exposed histidine ligands of the type-1 cu sites in five proteins, thiobacillus ferrooxidans rusticyanin, pseudomonas aeruginosa azurin, fern plastocyanin, alcaligenes faecalis pseudoazurin, and paracoccus versutus amicyanin, were studied with quantum chemical methods and conductorlike polarizable continuum model (cpcm). active site model molecules consisting of approximately 140 atoms were extracted from x-ray crystal structures and optimized with the homogeneous ... | 2010 | 20000723 |
| method to detect only viable cells in microbial ecology. | propidium monoazide can limit the analysis of microbial communities derived from genetic fingerprints to viable cells with intact cell membranes. however, pma treatment cannot completely suppress polymerase chain reaction (pcr) amplification when the targeted gene is too short. pma treatment in combination with two-step nested pcr was designed to overcome this problem. four experiments were performed to determine the limitation of pma treatment and to evaluate the suitability of the method by ap ... | 2010 | 20024544 |
| actinobaculum schaalii, a common uropathogen in elderly patients, denmark. | actinobaculum schaalii can cause urinary tract infections and septicemia but is difficult to identify by cultivation. to obtain a fast diagnosis and identify a. schaalii, we developed a taqman real-time quantitative pcr. routine urine samples were obtained from 177 hospitalized patients and 75 outpatients in viborg county, denmark, in 2008-2009. the pcr detected a. schaalii in 22% of samples from patients >60 years of age. this assay showed that a. schaalii is more common than implied by routine ... | 2010 | 20031046 |
| high-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is suitable for high-throughput and rapid diagnostics at low costs and can be considered an alternative for conventional biochemical and molecular identification systems in a conventional microbiological laboratory. first, we evaluated maldi-tof ms using 327 clinical isolates previously cultured from patient materials and identified by conventional techniques (vitek-ii, api, and biochemical tests). discre ... | 2010 | 20053859 |
| first reported case of alcaligenes faecalis peritonitis. | | 2010 | 20056996 |
| trends in antimicrobial susceptibility of gram-negative bacteria isolated from blood in jakarta from 2002 to 2008. | this study examined the susceptibility of gram-negative bacteria in the bloodstream to antimicrobials with the aim of providing information relevant to the guidance of therapy. | 2009 | 20061679 |