Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a prospective study of intrafamilial oropharyngeal transmission of kingella kingae. | to evaluate the intrafamilial oropharyngeal transmission of kingella kingae, we conducted a prospective study among pairs of siblings. we found that 55% of children who suffered from osteoarticular infections due to k. kingae, and 40% of asymptomatic carriers of k. kingae had siblings with positive oropharyngeal carriage. | 2014 | 24096731 |
| characterization of the kingella kingae polysaccharide capsule and exopolysaccharide. | recent evidence indicates that kingella kingae produces a polysaccharide capsule. in an effort to determine the composition and structure of this polysaccharide capsule, in the current study we purified capsular material from the surface of k. kingae strain 269-492 variant kk01 using acidic conditions to release the capsule and a series of steps to remove dna, rna, and protein. analysis of the resulting material by gas chromatography and mass spectrometry revealed n-acetyl galactosamine (galnac) ... | 2013 | 24098695 |
| recent lessons for the management of bone and joint infections. | the epidemiology and clinical manifestations of osteoarticular infections are changing primarily as a result of the emergence of community-acquired methicillin-resistant staphylococcus aureus infections. multifocal disease, venous thrombosis and pathologic fractures are manifestations of ca-mrsa osteomyelitis. mri is the diagnostic imaging modality of choice for musculoskeletal infections. nafcillin/oxacillin or cefazolin remains the antibiotic of choice for treating infections caused by mssa. a ... | 2014 | 24119927 |
| possible association of kingella kingae with infantile spondylodiscitis. | the course of the spondylodiscitis' infantile form is characterized by a mild-to-moderate clinical and biologic inflammatory response. unfortunately, blood and disk/vertebral aspiration cultures show a high percentage of negative results. however, detecting kingella kingae dna in the oropharynx provided reasonable suspicion, to our opinion, that this microorganism is responsible for the spondylodiscitis. | 2013 | 24131988 |
| systematic pcr detection in culture-negative osteoarticular infections. | identification of microorganisms is crucial for the successful treatment of osteoarticular infections. molecular methods are more sensitive than culture-dependent methods but may suffer from lack of specificity. | 2013 | 24135511 |
| letter to the editor: another look: is there a flaw to current hip septic arthritis diagnostic algorithms? | 2013 | 24136809 | |
| letter to the editor: another look: is there a flaw to current hip septic arthritis diagnostic algorithms? | 2013 | 24136809 | |
| reply to the letter to the editor: another look: is there a flaw to current hip septic arthritis diagnostic algorithms? | 2013 | 24146359 | |
| reply to the letter to the editor: another look: is there a flaw to current hip septic arthritis diagnostic algorithms? | 2013 | 24146359 | |
| patient-specific analysis of periodontal and peri-implant microbiomes. | periodontally involved teeth have been implicated as 'microbial reservoirs' in the etiology of peri-implant diseases. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the degree of congruence between adjacent peri-implant and periodontal microbiomes in states of health and disease. subgingival and peri-implant biofilm samples were collected from 81 partially edentulous individuals with periodontal and peri-implant health and disease. bacterial dna wa ... | 2013 | 24158341 |
| clinical features of candidiasis in patients with inherited interleukin 12 receptor β1 deficiency. | interleukin 12rβ1 (il-12rβ1)-deficient patients are prone to clinical disease caused by mycobacteria, salmonella, and other intramacrophagic pathogens, probably because of impaired interleukin 12-dependent interferon γ production. about 25% of patients also display mucocutaneous candidiasis, probably owing to impaired interleukin 23-dependent interleukin 17 immunity. the clinical features and outcome of candidiasis in these patients have not been described before, to our knowledge. we report her ... | 2013 | 24186907 |
| clinical features of candidiasis in patients with inherited interleukin 12 receptor β1 deficiency. | interleukin 12rβ1 (il-12rβ1)-deficient patients are prone to clinical disease caused by mycobacteria, salmonella, and other intramacrophagic pathogens, probably because of impaired interleukin 12-dependent interferon γ production. about 25% of patients also display mucocutaneous candidiasis, probably owing to impaired interleukin 23-dependent interleukin 17 immunity. the clinical features and outcome of candidiasis in these patients have not been described before, to our knowledge. we report her ... | 2013 | 24186907 |
| infective endocarditis by aggregatibacter paraphrophilus: case report and literature review. | aggregatibacter paraphrophilus (former name, haemophilus paraphrophilus) is a normal inhabitant of the naso- and oropharynx and has been rarely reported as a cause of human infections. a case of infective endocarditis by this organism is being reported and literature of endocarditis cases caused by aggregatibacter paraphrophilus is being reviewed. | 2013 | 24392406 |
| a pediatric case of cardiobacterium hominis endocarditis. | gram negative endocarditis is relatively rare in pediatrics but when they occur they are most frequently caused by one of the hacek (haemophilus species, actinobacillus actinomycetemcomitans, c. hominis, eikenella corrodens and kingella kingae) group of microorganisms. within the hacek group of microorganisms there have been approximately 100 cases of cardiobacterium hominis endocarditis reported in the literature, but only 2 previous cases of endocarditis and one case of pericarditis have been ... | 2013 | 24470958 |
| kingella kingae septic arthritis in children: recognising an elusive pathogen. | kingella kingae is an increasingly identified cause of musculoskeletal infections in young children. we report our experience with a recently developed polymerase chain reaction (pcr) method and review the clinical course of children diagnosed with k. kingae septic arthritis in a tertiary referral paediatric hospital. | 2014 | 24488842 |
| 'bobo-newton syndrome': an unwanted gift from man's best friend. | capnocytophaga canimorsus is a facultative gram-negative bacillus that is typically a constituent of the oral flora of dogs and cats. it was first isolated by bobo and newton in 1976 from a man presenting with meningitis following a dog bite. transmission to humans follows various animal-related injuries, which may be gross or subtle. c canimorsus can cause a spectrum of syndromes ranging from skin and soft tissue infection to invasive disease such as meningitis or endocarditis. the present arti ... | 2013 | 24489563 |
| [kingella kingae sternoclavicular septic arthritis in an adult]. | 2014 | 24556455 | |
| surgical treatment of infective endocarditis in active intravenous drug users: a justified procedure? | infective endocarditis is a life threatening complication of intravenous drug abuse, which continues to be a major burden with inadequately characterised long-term outcomes. we reviewed our institutional experience of surgical treatment of infective endocarditis in active intravenous drug abusers with the aim of identifying the determinants long-term outcome of this distinct subgroup of infective endocarditis patients. | 2014 | 24661344 |
| rtx toxin plays a key role in kingella kingae virulence in an infant rat model. | kingella kingae is a human oral bacterium that can cause diseases of the skeletal system in children and infective endocarditis in children and adults. k. kingae produces a toxin of the rtx group, rtxa. to investigate the role of rtxa in disease pathogenesis in vivo, k. kingae strain pykk081 and its isogenic rtxa-deficient strain kknb100 were tested for their virulence and pathological consequences upon intraperitoneal injections in 7-day-postnatal (pn 7) rats. at the doses above 8.0 × 10(6) cel ... | 2014 | 24664507 |
| classification, identification, and clinical significance of haemophilus and aggregatibacter species with host specificity for humans. | the aim of this review is to provide a comprehensive update on the current classification and identification of haemophilus and aggregatibacter species with exclusive or predominant host specificity for humans. haemophilus influenzae and some of the other haemophilus species are commonly encountered in the clinical microbiology laboratory and demonstrate a wide range of pathogenicity, from life-threatening invasive disease to respiratory infections to a nonpathogenic, commensal lifestyle. new sp ... | 2014 | 24696434 |
| the hmw1c-like glycosyltransferases--an enzyme family with a sweet tooth for simple sugars. | 2014 | 24722584 | |
| outbreaks of kingella kingae infections in daycare facilities. | during the past decade, transmission of the bacterium kingella kingae has caused clusters of serious infections, including osteomyelitis, septic arthritis, bacteremia, endocarditis, and meningitis, among children in daycare centers in the united states, france, and israel. these events have been characterized by high attack rates of disease and prevalence of the invasive strain among asymptomatic classmates of the respective index patients, suggesting that the causative organisms benefitted from ... | 2014 | 24750782 |
| genotyping, local prevalence and international dissemination of β-lactamase-producing kingella kingae strains. | β-lactamase production has been sporadically reported in the emerging kingella kingae pathogen but the phenomenon has not been studied in-depth. we investigated the prevalence of β-lactamase production among k. kingae isolates from different geographical origins and genetically characterized β-lactamase-producing strains. seven hundred and seventy-eight isolates from iceland, the usa, france, israel, spain and canada were screened for β-lactamase production and, if positive, were characterized b ... | 2014 | 24766502 |
| predicting the occurrence of embolic events: an analysis of 1456 episodes of infective endocarditis from the italian study on endocarditis (sei). | embolic events are a major cause of morbidity and mortality in patients with infective endocarditis. we analyzed the database of the prospective cohort study sei in order to identify factors associated with the occurrence of embolic events and to develop a scoring system for the assessment of the risk of embolism. | 2014 | 24779617 |
| high rates of complications following kingella kingae infective endocarditis in children: a case series and review of the literature. | kingella kingae is part of the haemophilus spp., aggregatibacter spp., cardiobacterium hominis, eikenella corrodens and kingella spp. organisms that are known to cause bacterial endocarditis. evidence suggests it is also a common pharyngeal colonizer in children <2 years of age. we reviewed the literature to determine common complications of k. kingae infective endocarditis in children. | 2014 | 24921624 |
| clinical features of right-sided infective endocarditis occurring in non-drug users. | right-sided infective endocarditis (rie) occurs predominantly in intravenous drug users in western countries, and it has a relatively good prognosis. clinical features and prognosis of rie occurring in non-drug users are not well known. we investigated the clinical findings of rie in non-drug users. we retrospectively reviewed 345 cases diagnosed with ie. cases with rie or left-sided infective endocarditis (lie) defined by the vegetation site were included and cases having no vegetation or both- ... | 2014 | 24932077 |
| anti-biofilm activity: a function of klebsiella pneumoniae capsular polysaccharide. | competition and cooperation phenomena occur within highly interactive biofilm communities and several non-biocides molecules produced by microorganisms have been described as impairing biofilm formation. in this study, we investigated the anti-biofilm capacities of an ubiquitous and biofilm producing bacterium, klebsiella pneumoniae. cell-free supernatant from k. pneumoniae planktonic cultures showed anti-biofilm effects on most gram positive bacteria tested but also encompassed some gram negati ... | 2014 | 24932475 |
| evaluation of a real-time pcr assay for simultaneous detection of kingella kingae and staphylococcus aureus from synovial fluid in suspected septic arthritis. | direct plating of synovial fluid (sf) on agar-based media often fails to identify pathogens in septic arthritis (sa). we developed a pcr assay for the simultaneous detection of kingella kingae and staphylococcus aureus from sf to evaluate molecular detection in sf and to estimate the incidence of k. kingae in sa in north america. the assay was based on detection of the cpn60 gene of k. kingae and the spa gene of s. aureus in multiplex real-time pcr. k. kingae was identified in 50% of patients be ... | 2014 | 24982837 |
| first identification of a chromosomally located penicillinase gene in kingella kingae species isolated in continental europe. | kingella kingae is the major pathogen causing osteoarticular infections (oai) in young children in numerous countries. plasmid-borne tem-1 penicillinase production has been sporadically detected in a few countries but not in continental europe, despite a high prevalence of k. kingae infections. we describe here for the first time a k. kingae β-lactamase-producing strain in continental europe and demonstrate the novel chromosomal location of the blatem-1 gene in k. kingae species. | 2014 | 25049250 |
| kingella kingae sequence type-complex 14 arthritis in a 16-month-old child in greece. | we describe the first case of kingella kingae arthritis in a 16-month-old girl in greece, which has been diagnosed by novel molecular techniques. a joint aspiration of her knee was performed before the initiation of antibiotics, as well as on the 5th and 14th day of empiric antimicrobial therapy. the synovial fluid white blood cell count decreased from 65,000 to 1500 cells/mm, but the percentage of neutrophils remained 90% in all 3 specimens. molecular analysis of the synovial fluid specimens by ... | 2015 | 25105896 |
| major intercontinentally distributed sequence types of kingella kingae and development of a rapid molecular typing tool. | although kingella kingae is the most common etiology of osteoarticular infections in young children, is a frequent cause of bacteremia in those younger than 4 years, and has been involved in clusters of invasive infections among daycare center attendees, the population structure of the species has not been systematically studied. using multilocus sequence typing, we investigated the genetic diversity of the largest intercontinental collection of k. kingae strains to date. to facilitate typing of ... | 2014 | 25143574 |
| kingella kingae dna in langerhans cell histiocytosis of bone. | langerhans cell histiocytosis of bone is a rare pediatric neoplastic disorder of unclear pathogenesis. we report the case of a 3-year-old girl who presented with langerhans cell histiocytosis of the ilium in which kingella kingae was detected. our findings argue for the search for k. kingae by polymerase chain reaction in children with langerhans cell histiocytosis of bone. | 2015 | 25144797 |
| calcium-enhanced twitching motility in xylella fastidiosa is linked to a single pily1 homolog. | the plant-pathogenic bacterium xylella fastidiosa is restricted to the xylem vessel environment, where mineral nutrients are transported through the plant host; therefore, changes in the concentrations of these elements likely impact the growth and virulence of this bacterium. twitching motility, dependent on type iv pili (tfp), is required for movement against the transpiration stream that results in basipetal colonization. we previously demonstrated that calcium (ca) increases the motility of ... | 2014 | 25217013 |
| differentiating kingella kingae septic arthritis of the hip from transient synovitis in young children. | to conduct a retrospective multicenter study to assess the ability of a predictive algorithm to differentiate between children with kingella kingae infection of the hip and those with transient synovitis. | 2014 | 25217199 |
| primary epiphyseal or apophyseal subacute osteomyelitis in the pediatric population: a report of fourteen cases and a systematic review of the literature. | primary epiphyseal or apophyseal subacute osteomyelitis (peasao) is a rare condition that typically has mild symptoms and lack of a systemic reaction, according to opinions, case reports, and case series. we reviewed fourteen consecutive cases of peasao treated at our institution over a thirteen-year period to characterize this disorder. | 2014 | 25232082 |
| lactobacillus endocarditis with prosthetic material: a case report on non-surgical management with corresponding literature review. | lactobacilli are rod shaped gram positive bacteria that naturally colonize the human gastrointestinal and genitourinary tracts and occasionally cause disease in humans. lactobacillus infections are found in patients who are immunocompromized or have severe comorbidities. we report lactobacillus endocarditis in a 17-year-old adolescent girl with cardiac prosthetic material following surgical correction for complex cyanotic congenital heart disease. accurate identification of the organism can be d ... | 2014 | 25276330 |
| [septic arthritis in children with normal initial c-reactive protein: clinical and biological features]. | septic arthritis has to be suspected in children with joint effusion and fever so as to perform joint aspiration, which will confirm the diagnosis by bacteriological methods, and to perform surgical treatment by joint lavage. since development of current molecular methods, such as real-time pcr, kingella kingae has become the first microbial agent of osteoarticular infections in young children, whereas staphylococcus aureus is second. c-reactive protein (crp) is an aid used to diagnose septic ar ... | 2014 | 25282461 |
| complementarity between targeted real-time specific pcr and conventional broad-range 16s rdna pcr in the syndrome-driven diagnosis of infectious diseases. | molecular tools have shown an added value in the diagnosis of infectious diseases, in particular for those caused by fastidious intracellular microorganisms, or in patients receiving antibiotics before sampling. if 16s rdna amplification had been gradually implemented in microbiology laboratories, specific real-time polymerase chain reaction (pcr) would have permitted an increase in the sensitivity of molecular methods and a reduction of contamination. herein, we report our experience in the dia ... | 2015 | 25348607 |
| chondrosternal arthritis in infant: an unusual entity. | primary arthritis of chondrosternal joint is very rare and occurs in infants less than 18 months of age. presentation is most often subacute but may be acute. child presents with a parasternal mass with history of fever and/or local signs of infection. clinical symptoms vary from a painless noninflammatory to a painful mass with local tenderness and swelling, while fever may be absent. laboratory data show low or marginally raised levels of white blood cells and c-reactive protein, reflecting, r ... | 2014 | 25386377 |
| first human systemic infection caused by spiroplasma. | spiroplasma species are organisms that normally colonize plants and insects. we describe the first case of human systemic infection caused by spiroplasma bacteria in a patient with hypogammaglobulinemia undergoing treatment with biological disease-modifying antirheumatic agents. spiroplasma turonicum was identified through molecular methods in several blood cultures. the infection was successfully treated with doxycycline plus levofloxacin. | 2014 | 25428150 |
| first human systemic infection caused by spiroplasma. | spiroplasma species are organisms that normally colonize plants and insects. we describe the first case of human systemic infection caused by spiroplasma bacteria in a patient with hypogammaglobulinemia undergoing treatment with biological disease-modifying antirheumatic agents. spiroplasma turonicum was identified through molecular methods in several blood cultures. the infection was successfully treated with doxycycline plus levofloxacin. | 2014 | 25428150 |
| kingella kingae kk247, an atypical pulsed-field gel electrophoresis clone a strain. | kingella kingae strain kk247 was isolated from an adult israeli patient with endocarditis. it belongs to pulsed-field gel electrophoresis clone a, has a 2,113,021-bp genome, a 15,507-bp plasmid that carries genes encoding β-lactamases, and possesses 45 transposases, compared to the 5 detected in other k. kingae strains. | 2014 | 25428974 |
| impact of calcium signaling during infection of neisseria meningitidis to human brain microvascular endothelial cells. | the pili and outer membrane proteins of neisseria meningitidis (meningococci) facilitate bacterial adhesion and invasion into host cells. in this context expression of meningococcal pilc1 protein has been reported to play a crucial role. intracellular calcium mobilization has been implicated as an important signaling event during internalization of several bacterial pathogens. here we employed time lapse calcium-imaging and demonstrated that pilc1 of meningococci triggered a significant increase ... | 2014 | 25464500 |
| aggregatibacter actinomycetemcomitans: virulence of its leukotoxin and association with aggressive periodontitis. | periodontitis is an infection-induced inflammatory disease that causes loss of the tooth supporting tissues. much focus has been put on comparison of the microbial biofilm in the healthy periodontium with the diseased one. the information arising from such studies is limited due to difficulties to compare the microbial composition in these two completely different ecological niches. a few longitudinal studies have contributed with information that makes it possible to predict which individuals w ... | 2014 | 25494963 |
| aggregatibacter actinomycetemcomitans: virulence of its leukotoxin and association with aggressive periodontitis. | periodontitis is an infection-induced inflammatory disease that causes loss of the tooth supporting tissues. much focus has been put on comparison of the microbial biofilm in the healthy periodontium with the diseased one. the information arising from such studies is limited due to difficulties to compare the microbial composition in these two completely different ecological niches. a few longitudinal studies have contributed with information that makes it possible to predict which individuals w ... | 2014 | 25494963 |
| penicillinase-encoding gene blatem-1 may be plasmid borne or chromosomally located in kingella kingae species. | 2015 | 25512412 | |
| peritoneal dialysis-related peritonitis due to kingella denitrificans: the first case report. | 2014 | 25520495 | |
| sequelae of pediatric osteoarticular infection. | the epidemiology and diagnosis of osteoarticular infections (oai) have changed considerably in recent years, partly due to the development of molecular biology. kingella kingae is now recognized as the most frequent pathogen in children under 4 years of age, while methicillin-resistant staphylococcus aureus (sa) has been increasingly reported. although the clinical course of oai is mostly benign, with shorter antibiotic regimens and simplified treatments, serious functional impairments and life- ... | 2015 | 25553604 |
| kingella kingae: carriage, transmission, and disease. | kingella kingae is a common etiology of pediatric bacteremia and the leading agent of osteomyelitis and septic arthritis in children aged 6 to 36 months. this gram-negative bacterium is carried asymptomatically in the oropharynx and disseminates by close interpersonal contact. the colonized epithelium is the source of bloodstream invasion and dissemination to distant sites, and certain clones show significant association with bacteremia, osteoarthritis, or endocarditis. kingella kingae produces ... | 2015 | 25567222 |
| the rebirth of culture in microbiology through the example of culturomics to study human gut microbiota. | bacterial culture was the first method used to describe the human microbiota, but this method is considered outdated by many researchers. metagenomics studies have since been applied to clinical microbiology; however, a "dark matter" of prokaryotes, which corresponds to a hole in our knowledge and includes minority bacterial populations, is not elucidated by these studies. by replicating the natural environment, environmental microbiologists were the first to reduce the "great plate count anomal ... | 2015 | 25567229 |
| high respiratory virus oropharyngeal carriage rate during kingella kingae osteoarticular infections in children. | kingella kingae osteoarticular (kko) infections are frequently associated with upper respiratory tract infections. however, no comparative studies detecting respiratory viruses had ever been performed between kko and non-kko (nko). | 2015 | 25598333 |
| a case of suppurative peritonitis by a commensal oral organism, kingella denitrificans, in an adult peritoneal dialysis patient. | 2015 | 25700466 | |
| a rare pathogen for subacute osteomyelitis in adolescent: serratia marcescens. | there are various pathogens reported for osteomyelitis. osteomyelitis is bone infection which produces pain and fever, also threatens bone instability. it can lead to nonunion. the purpose of this report was to describe a case with union delay of the tibia due to serratia marcescens osteomyelitis. serratia marcescens is an unexpected pathogen for subacute osteomyelitis in adolescence. because of difficulty of diagnosis, treatment can be delayed or the situation can cause complications like nonun ... | 2014 | 25704404 |
| a rare pathogen for subacute osteomyelitis in adolescent: serratia marcescens. | there are various pathogens reported for osteomyelitis. osteomyelitis is bone infection which produces pain and fever, also threatens bone instability. it can lead to nonunion. the purpose of this report was to describe a case with union delay of the tibia due to serratia marcescens osteomyelitis. serratia marcescens is an unexpected pathogen for subacute osteomyelitis in adolescence. because of difficulty of diagnosis, treatment can be delayed or the situation can cause complications like nonun ... | 2014 | 25704404 |
| [septic arthritis caused by kingella kingae in children]. | approximately 50% of cases of bacterial arthritis occur in childhood. currently kingella kingae is considered as an emerging pathogen. | 2015 | 25727838 |
| aetiology of arthritis in hospitalised children: an observational study. | arthritis in children has many causes and includes septic and viral arthritis, reactive arthritis and juvenile idiopathic arthritis (jia). we aimed to describe the different types of arthritis among children hospitalised for a first episode of arthritis. | 2015 | 25732253 |
| an outbreak of kingella kingae infections associated with hand, foot and mouth disease/herpangina virus outbreak in marseille, france, 2013. | outbreaks of invasive kingella kingae infections recently emerged as a new public health concern in daycare centers in europe, usa and israel. despite this, no trigger factor has been yet identified, preventing the setting up of rational measures of control and prevention. we report an outbreak of k. kingae infections associated with hand, foot and mouth disease/herpangina outbreak, and we define the research and policy priorities. | 2015 | 25742075 |
| recurrent infective endocarditis due to aggregatibacter aphrophilus and staphylococcus lugdunensis. | uncommon microorganisms are increasingly being recognized as causative agents of paediatric infectious endocarditis (ie). we report a 4-year old girl with congenital heart disease, who suffered from 2 ie episodes secondary to aggregatibacter aphrophilus (formerly haemophilus aphrophilus) and staphylococcus lugdunensis, both rarely reported pathogens in this age group. the patient was initially successfully treated with prolonged intravenous antibiotic courses, however removal of the contegra val ... | 2015 | 25751682 |
| pore forming activity of the potent rtx-toxin produced by pediatric pathogen kingella kingae: characterization and comparison to other rtx-family members. | pediatric septic arthritis in patients under age of four is frequently caused by the oral gram-negative bacterium kingella kingae. this organism may be responsible for a severe form of infective endocarditis in otherwise healthy children and adults. a major virulence factor of k. kingae is rtxa, a toxin that belongs to the rtx (repeats-in-toxin) group of secreted pore forming toxins. to understand the rtxa effects on host cell membranes, the toxin activity was studied using planar lipid bilayers ... | 2015 | 25858109 |
| hand, foot and mouth disease and kingella kingae infections. | 2015 | 25876094 | |
| first human case of meningitis and sepsis in a child caused by actinobacillus suis or actinobacillus equuli. | we report the first human case of meningitis and sepsis caused in a child by actinobacillus suis or a. equuli, a common opportunistic pathogen of swine or horses, respectively. identification was performed by matrix-assisted laser desorption ionization-time of flight mass spectrometry and real-time pcr assay. a previous visit to a farm was suspected as the source of infection. | 2015 | 25878346 |
| the clinical usefulness of polymerase chain reaction as a supplemental diagnostic tool in the evaluation and the treatment of children with septic arthritis. | culture-negative septic arthritis occurs frequently in children. the supplemental use of polymerase chain reaction (pcr) techniques improves the detection of bacteria in the joint fluid. this study evaluates the clinical utility of pcr at a tertiary pediatric medical center. | 2016 | 25887824 |
| clinical evaluation of commercial nucleic acid amplification tests in patients with suspected sepsis. | sepsis is a serious medical condition requiring timely administered, appropriate antibiotic therapy. blood culture is regarded as the gold standard for aetiological diagnosis of sepsis, but it suffers from low sensitivity and long turnaround time. thus, nucleic acid amplification tests (naats) have emerged to shorten the time to identification of causative microbes. the aim of the present study was to evaluate the clinical utility in everyday practice in the emergency department of two commercia ... | 2015 | 25928122 |
| [osteomyelitis: a probable, uncommon etiology agent]. | the relation of infectious agents to arthritis is an area of great interest to the rheumatologist. septic arthritis of bacterial origin accounts for approximately 6.5% of all childhood arthritides. septic arthritis usually results from haematogenous spread from a focus of infection elsewhere in the body, but also by direct extension of an infection from overlying soft tissues or bone or traumatic invasion of the joint. as a result, if a focus of underlying osteomyelitis breaks throught the metap ... | 2015 | 25941133 |
| evaluation of unbiased next-generation sequencing of rna (rna-seq) as a diagnostic method in influenza virus-positive respiratory samples. | unbiased nontargeted metagenomic rna sequencing (umers) has the advantage to detect known as well as unknown pathogens and, thus, can significantly improve the detection of viral, bacterial, parasitic, and fungal sequences in public health settings. in particular, conventional diagnostic methods successfully identify the putative pathogenic agent in only 30% to 40% of respiratory specimens from patients with acute respiratory illness. here, we applied umers to 24 diagnostic respiratory specimens ... | 2015 | 25972420 |
| development of two real-time multiplex pcr assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections. | the frequent lack of a positive and timely microbiological diagnosis in patients with lower respiratory tract infection (lrti) is an important obstacle to antimicrobial stewardship. patients are typically prescribed broad-spectrum empirical antibiotics while microbiology results are awaited, but, because these are often slow, negative, or inconclusive, de-escalation to narrow-spectrum agents rarely occurs in clinical practice. the aim of this study was to develop and evaluate two multiplex real- ... | 2015 | 25980353 |
| concomitant septic arthritis and osteomyelitis of the hip in young children; a new pathophysiological hypothesis suggested by mri enhancement pattern. | in children, septic arthritis (sa) of the hip is either primary or concomitant with acute haematogenous osteomyelitis (aho). however, seldom, patients with isolated sa at presentation, may later show osteomyelitis in the metaphysis. the aim of this study was to elaborate a physiopathological hypothesis based on the peculiar mri findings to explain the onset of aho after sa. | 2015 | 25986395 |
| staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. | staphylococcus aureus is a major human pathogen that causes a wide range of clinical infections. it is a leading cause of bacteremia and infective endocarditis as well as osteoarticular, skin and soft tissue, pleuropulmonary, and device-related infections. this review comprehensively covers the epidemiology, pathophysiology, clinical manifestations, and management of each of these clinical entities. the past 2 decades have witnessed two clear shifts in the epidemiology of s. aureus infections: f ... | 2015 | 26016486 |
| in brief: kanavel's signs and pyogenic flexor tenosynovitis. | 2015 | 26022113 | |
| in brief: kanavel's signs and pyogenic flexor tenosynovitis. | 2015 | 26022113 | |
| polyarticular septic arthritis caused by haemophilus influenzae serotype f in an 8-month-old immunocompetent infant: a case report and review of the literature. | background. the standard use of vaccinations against pathogens has resulted in a decreased incidence of musculoskeletal infections caused by these previously common bacterial pathogens. consequently, the incidence of infections caused by atypical bacteria is rising. this report presents a case of septic arthritis caused by non-type b h. influenzae in a pediatric patient. methods. we report a case of an infant with polyarticular septic arthritis caused by h. influenzae serotype f. a literature re ... | 2015 | 26064739 |
| bone and joint infections in children: acute hematogenous osteomyelitis. | acute hematogenous osteomyelitis (aho) is one of the commonest bone infection in childhood. staphylococcus aureus is the commonest organism causing aho. with use of advanced diagnostic methods, fastidious kingella kingae is increasingly becoming an important organism in etiology of osteoarticular infections in children under the age of 3 y. the diagnosis of aho is primarily clinical. the main clinical symptom and sign in aho is pain and tenderness over the affected bone especially in the metaphy ... | 2016 | 26096866 |
| septic cerebral embolisation in fulminant mitral valve infective endocarditis. | a 37-year-old male with known intravenous drug use was admitted with an acute onset of worsening confusion and speech impairment. his vitals and biochemical profile demonstrated severe sepsis, with a brain ct showing several lesions suspicious for cerebral emboli. he then went on to have a bedside transthoracic echocardiogram that was positive for vegetation on the mitral valve, with associated severe mitral regurgitation. unfortunately, before he was stable enough to be transferred for valve su ... | 2015 | 26120312 |
| identification of a conserved linear b-cell epitope of streptococcus dysgalactiae gapc protein by screening phage-displayed random peptide library. | the gapc of streptococcus dysgalactiae (s. dysgalactiae) is a highly conserved surface protein that can induce protective humoral immune response in animals. however, b-cell epitopes on the s. dysgalactiae gapc have not been well identified. in this study, a monoclonal antibody (mab5b7) against the gapc1-150 protein was prepared. after passive transfer, mab5b7 could partially protect mice against s. dysgalactiae infection. eleven positive phage clones recognized by mab5b7 were identified by scre ... | 2015 | 26121648 |
| the influence of smoking on the peri-implant microbiome. | smokers are at high risk for 2 bacterially driven oral diseases: peri-implant mucositis and peri-implantitis. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the effect of smoking on the peri-implant microbiome in states of health and disease. peri-implant biofilm samples were collected from 80 partially edentulous subjects with peri-implant health, peri-implant mucositis, and peri-implantitis. bacterial dna was isolated and 16s ribsomal rna gene li ... | 2015 | 26124222 |
| bacteriological assessment of the hospital environment in two referral hospitals in yaoundé-cameroon. | many studies still show significant numbers of surgical patients contracting nosocomial infections each year globally with high morbidity and mortality. the aim of this study was to identify potential bacteria reservoirs that may be responsible for nosocomial infection in surgical services in the yaoundé university teaching hospital (yuth) and the central hospital yaoundé (chy). | 2015 | 26140067 |
| kingella kingae infections in children. | improvements in culture techniques and molecular detection methods have led to findings indicating that, particularly in infants and young children, kingella kingae is a significantly more important pathogen than previously thought. however, despite this, the pediatric community is still largely unaware of the existence of this organism. the aim of this review is therefore to summarise current knowledge of the epidemiology, transmission, clinical presentation, diagnosis and treatment of k. kinga ... | 2015 | 26148872 |
| pattern recognition receptors in microbial keratitis. | microbial keratitis is a significant cause of global visual impairment and blindness. corneal infection can be caused by a wide variety of pathogens, each of which exhibits a range of mechanisms by which the immune system is activated. the complexity of the immune response to corneal infection is only now beginning to be elucidated. crucial to the cornea's defences are the pattern-recognition receptors: toll-like and nod-like receptors and the subsequent activation of inflammatory pathways. thes ... | 2015 | 26160532 |
| oropharyngeal kingella kingae carriage in children: characteristics and correlation with osteoarticular infections. | the aim of this study was to investigate changes in oropharyngeal k. kingae carriage during the first 4 y of life, including seasonal variation and comparison of asymptomatic carriage with cases of invasive osteoarticular infections (oai). | 2015 | 26186293 |
| kingella kingae causing septic arthritis of the knee in an immunocompetent adult. | the bacterium kingella kingae is a species of gram-negative coccobacillus usually found in the oropharynx. this is an emerging pathogen reported to cause bacteraemia, endocarditis, and osteoarticular infections in children and endocarditis in the immunocompromised adult. however, there are few cases of isolated joint infections reported in the immunocompetent adult. due to specific isolation techniques required, delay in diagnosis can compromise patient outcome. we report a rare case of septic a ... | 2015 | 26199777 |
| nontuberculous mycobacteria: an underestimated cause of bioprosthetic valve infective endocarditis. | background. atypical mycobacteria, or nontuberculous mycobacteria (ntm), have been barely reported as infective endocarditis (ie) agents. methods. from january 2010 to december 2013, cardiac valve samples sent to our laboratory as cases of blood culture-negative suspected ie were analyzed by 16s rdna polymerase chain reaction (pcr). when positive for ntm, hsp pcr allowed species identification. demographic, clinical, echocardiographic, histopathological, and ziehl-neelsen staining data were th ... | 2015 | 26213691 |
| paediatric musculoskeletal interventional radiology. | interventional radiology technique is now well established and widely used in the adult population. through minimally invasive procedures, it increasingly replaces surgical interventions that involve higher percentages of invasiveness and, consequently, of morbidity and mortality. for these advantageous reasons, interventional radiology in recent years has spread to the paediatric age as well. the aim of this study was to review the literature on the development, use and perspectives of these pr ... | 2015 | 26235144 |
| paediatric musculoskeletal interventional radiology. | interventional radiology technique is now well established and widely used in the adult population. through minimally invasive procedures, it increasingly replaces surgical interventions that involve higher percentages of invasiveness and, consequently, of morbidity and mortality. for these advantageous reasons, interventional radiology in recent years has spread to the paediatric age as well. the aim of this study was to review the literature on the development, use and perspectives of these pr ... | 2015 | 26235144 |
| epidemiology and the prognosis of healthcare-associated infective endocarditis in china: the significance of non-nosocomial acquisition. | limited research has been conducted on healthcare-associated infective endocarditis (haie), although it is of increasing importance. the aim of this study is to compare the epidemiology, clinical characteristics, and prognosis of community-acquired ie (ca-ie) with haie and non-nosocomial healthcare-associated ie (nnhca-ie). a retrospective, consecutive case-series analysis was organized and performed during the 20-year study period in huashan hospital, shanghai, china. a total of 154 patients we ... | 2015 | 26251828 |
| [mycotic aneurysm of the ascending aorta and cerebral infarcts in a 17-month old child with kingella kingae endocarditis]. | endocarditis is an uncommon presentation of kingella kingae infection in children. a previously healthy 17 month old child was referred to our emergency department for evaluation of fever lasting eleven days, aphthous stomatitis and a new systolic murmur. within a few hours of admission, antibiotic therapy was initiated for a presumptive diagnosis of bacteremia and within 24 hours after admission, gram negative coccobacilli were growing in the blood culture. in addition, echocardiography demonst ... | 2015 | 26281080 |
| unconventional n-linked glycosylation promotes trimeric autotransporter function in kingella kingae and aggregatibacter aphrophilus. | glycosylation is a widespread mechanism employed by both eukaryotes and bacteria to increase the functional diversity of their proteomes. the nontypeable haemophilus influenzae glycosyltransferase hmw1c mediates unconventional n-linked glycosylation of the adhesive protein hmw1, which is encoded in a two-partner secretion system gene cluster that also encodes hmw1c. in this system, hmw1 is modified in the cytoplasm by sequential transfer of hexose residues. in the present study, we examined king ... | 2015 | 26307167 |
| cartilage matrix infection in young children by kingella kingae. | 2015 | 26315207 | |
| invasive kingella kingae resulting in a brodie abscess. | 2015 | 26407421 | |
| susceptibility pattern of microorganisms isolated by percutaneous needle biopsy in nonbacteremic pyogenic vertebral osteomyelitis. | pyogenic vertebral osteomyelitis (vo) is diagnosed according to several lines of evidence: clinical, biological, radiological, and histological. definitive diagnosis requires the isolation of a causative pathogen or histological confirmation. the aim of our study was to describe the microorganisms isolated by percutaneous needle biopsy (pnb) and to analyze their susceptibility patterns, in order to assess the possibility of empirical combination therapy for the treatment of nonbacteremic patient ... | 2015 | 26438497 |
| what to expect from molecular tools for non-documented pediatric infectious diseases. | evaluation of the contribution of molecular tools to the overall diagnosis of infectious diseases in children. | 2015 | 26506055 |
| outbreaks of invasive kingella kingae infections in closed communities. | to describe the results of the epidemiologic investigation of outbreaks of invasive kingella kingae infections among attendees at daycare facilities located in 4 closed communities in israel. | 2016 | 26545728 |
| genome analysis of kingella kingae strain kwg1 reveals how a β-lactamase gene inserted in the chromosome of this species. | we describe the genome of a penicillinase-producing kingella kingae strain (kwg1), the first to be isolated in continental europe, whose bla(tem-1) gene was, for the first time in this species, found to be chromosomally inserted. the bla(tem) gene is located in an integrative and conjugative element (ice) inserted in met-trna and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. this ice is homologous to resistance-conferring plasmids of k. kingae and other ... | 2015 | 26574009 |
| genome analysis of kingella kingae strain kwg1 reveals how a β-lactamase gene inserted in the chromosome of this species. | we describe the genome of a penicillinase-producing kingella kingae strain (kwg1), the first to be isolated in continental europe, whose bla(tem-1) gene was, for the first time in this species, found to be chromosomally inserted. the bla(tem) gene is located in an integrative and conjugative element (ice) inserted in met-trna and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. this ice is homologous to resistance-conferring plasmids of k. kingae and other ... | 2015 | 26574009 |
| kingella kingae corneal infections in children. | 2014 | 26624911 | |
| age-dependent carriage of kingella kingae in young children and turnover of colonizing strains. | in a longitudinal study, kingella kingae carriage rate was nil below 6 months of age, 1.5% at 6 months, 9.6% at 12 months, remained stable between 10.4% and 12.0% during the second year of life, and decreased significantly to 5.3% at 30 months. replacement of carried strains occurred over time. | 2014 | 26625369 |
| kingella kingae sequence type 25 causing endocarditis with multiple and severe cerebral complications. | 2016 | 26651429 | |
| patterns of kingella kingae disease outbreaks. | kingella kingae outbreaks occur sporadically in childcare centers but remain poorly understood and difficult to identify. | 2016 | 26658382 |
| evaluation of the bruker maldi biotyper for identification of fastidious gram-negative rods. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has entered clinical laboratories, facilitating identification of bacteria. here, we evaluated the maldi biotyper (bruker daltonics) for the identification of fastidious gram-negative rods (gnr). three sample preparation methods, direct colony transfer, direct transfer plus on-target formic acid preparation, and ethanol-formic acid extraction, were analyzed for 151 clinical isolates. direct colony transfe ... | 2016 | 26659214 |
| structural analysis of haemoglobin binding by hpua from the neisseriaceae family. | the neisseriaceae family of bacteria causes a range of diseases including meningitis, septicaemia, gonorrhoea and endocarditis, and extracts haem from haemoglobin as an important iron source within the iron-limited environment of its human host. herein we report crystal structures of apo- and haemoglobin-bound hpua, an essential component of this haem import system. the interface involves long loops on the bacterial receptor that present hydrophobic side chains for packing against the surface of ... | 2015 | 26671256 |
| genotyping and resolution of a case of osteomyelitis in a 16-month-old boy of hispanic/african american ethnicity. | most cases of osteomyelitis in children are caused by staphylococcus aureus, although kingella kingae, various streptococci, and salmonella species also underlie this condition. organisms such as mycobacterium, histoplasma, and cryptococcus are much less commonly identified as etiologic agents in osteomyelitis. this case report describes a 16-month-old boy of hispanic/african american ethnicity who had extensive inflammation of and discharge from his right ankle. imaging studies supported a diag ... | 2016 | 26715611 |