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kingella kingae endocarditis in a child with hair-cartilage hypoplasia.abstract. kingella kingae is a fastidious gram-negative rod that since the 1980s has been appreciated as a cause of a variety of human infections, including bone and joint infections, bacteremia, and rarely endocarditis [2, 6, 7, 9]. k. kingae endocarditis is rare, and only a few cases occur in normal, native valves. we report a case of k. kingae endocarditis in a patient with hair-cartilage hypoplasia who had previously undergone bone marrow transplantation. the combination of these rare condit ...19979326695
kingella kingae infections in paediatric patients: 5 cases of septic arthritis, osteomyelitis and bacteraemia.kingella kingae is a gram-negative rod most often recognized as 1 of the organisms causing septic arthritis and osteomyelitis in children. infection caused by k. kingae had not been diagnosed in iceland until 5 cases were diagnosed at the paediatric department at the university hospital of iceland over a 1 year period. in this report we describe these 5 children with invasive infection caused by k. kingae (2 with septic arthritis, 1 with osteomyelitis, 1 with septic arthritis and osteomyelitis, ...19979435039
kingella kingae endocarditis following chickenpox in infancy. 19979495681
increasing prevalence of kingella kingae in osteoarticular infections in young children.sixty children younger than 3 years with culture-positive hematogenous septic arthritis and acute/subacute osteomyelitis treated between 1990 and 1995 were reviewed to identify the infecting organism. gram-positive bacteria were identified in 47 (78.3%) patients, and gram-negative organisms were identified in 13 (21.7%) patients. haemophilus influenzae was cultured in none of the cases of septic arthritis and in only one (1.6%) case of acute osteomyelitis. kingella kingae was cultured in 10 (16. ...19989531414
molecular identification of gemella species from three patients with endocarditis.gemella morbillorum and gemella haemolysans are opportunistic pathogens which cause endocarditis and other severe infections. we report on three patients with endocarditis, one with endocarditis caused by g. haemolysans and two with endocarditis caused by g. morbillorum. the paucity of reports concerning these bacteria is probably related to the difficulties associated with their identification. for example, one of the strains reported in this study was originally sent to our laboratory with a p ...19989542900
[osteomyelitis of the astragalus caused by kingella kingae]. 19989666597
antibody response in six hacek endocarditis cases under therapy.the antibody response to bacteria of the so-called hacek group, i.e. haemophilus spp., actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens and kingella kingae, was measured in sera of six patients with endocarditis. the corresponding isolates from their blood cultures were identified by conventional methods, including reactions for nitrate reduction and catalase as well as acid production from sugars. crude antigens were prepared by glycine extraction and sonificat ...19989674892
invasive kingella kingae infection associated with stomatitis in children. 19989726357
five cases of kingella kingae skeletal infection in a french hospital.five cases of kingella kingae skeletal infections were diagnosed in children admitted to la timone hospital between 1992 and 1997. patients were between 6 and 31 months old and presented with septic spondylodiskitis, calcaneus osteomyelitis, and hip-joint arthritis. all displayed either an upper respiratory tract infection or eczema during the month prior to their admission. laboratory findings included an elevated leukocyte count and an elevated erythrocyte sedimentation rate. standard radiogra ...19989764556
use of the bact/alert blood culture system for culture of sterile body fluids other than blood.studies have demonstrated that large-volume culture methods for sterile body fluids other than blood increase recovery compared to traditional plated-medium methods. bact/alert is a fully automated blood culture system for detecting bacteremia and fungemia. in this study, we compared culture in bact/alert standard aerobic and anaerobic bottles, bact/alert fan aerobic and fan anaerobic bottles, and culture on routine media for six specimen types, i.e., continuous ambulatory peritoneal dialysate ( ...19989774578
growth differences of capnocytophaga canimorsus strains and some other fastidious organisms on various columbia-based blood agar media.three human isolates of capnocytophaga canimorsus from blood cultures showed no growth when cultivated on sheep blood agar plates based on bbl columbia agar base, whereas the same three strains grew excellently on sheep blood agar plates based on difco columbia agar bases no. 2 or eh. two of the three strains also grew well on blood agar plates based on biomérieux columbia agar. strains that grew better on either difco or biomérieux columbia based blood agar plates were found among eikenella cor ...19959810657
person-to-person transmission of kingella kingae among day care center attendees.fifty kingella kingae organisms, isolated from tonsillar cultures of day care center attendees during an 11-month period, and 60 isolates derived from epidemiologically unrelated individuals, including 19 isolates from respiratory carriers and 41 isolates from patients with invasive infections, were typed by immunoblotting, pulsed-field gel electrophoresis, and ribotyping. one strain, defined by unique immunoblotting, pulsed-field gel electrophoresis, and ribotyping patterns, represented 14 day ...19989815248
polymerase chain reaction diagnosis of kingella kingae arthritis in a young child. 19989827297
nested duplex pcr to detect bordetella pertussis and bordetella parapertussis and its application in diagnosis of pertussis in nonmetropolitan southeast queensland, australia.a duplex pcr to detect bordetella pertussis and bordetella parapertussis was developed with the insertion sequences is481 (b. pertussis) and is1001 (b. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. no culture-positive-pcr-negative results occurred, giving the method a sensitivity of 100%. for b. pertussis, 58 of 520 patients (11.2%) were positive by pcr compared to 17 of 520 patients positive (3.3%) by culture. for b. parapertussis, ...19999986820
etiology of septic arthritis in children: an update for the 1990s.to establish the etiology of septic arthritis in children after implementation of hib immunization guidelines.199910069312
recovery of kingella kingae from blood and synovial fluid of two pediatric patients by using the bact/alert system. 199910084890
[multifocal invasive kingella kingae infection].case report: a 2-year-old child, non immunodeficient, presented with septicemia due to kingella kingae successively complicated by meningitis, arthritis of one knee and endocarditis. outcome was favourable after a long and adjusted antibiotherapy, involving in particular for the endocarditis ceftriaxone (100 mg/kg/d) and amikacin (20 mg/kg/d) during 3 weeks, then amoxicillin per os (200 mg/kg/d) during 3 weeks. conclusions: bacteriologic characteristics of the bacteria, the culture of which requ ...199810223137
[osteoarticular infection by kingella kingae: two case reports]. 199910394189
gliding motility in bacteria: insights from studies of myxococcus xanthus.gliding motility is observed in a large variety of phylogenetically unrelated bacteria. gliding provides a means for microbes to travel in environments with a low water content, such as might be found in biofilms, microbial mats, and soil. gliding is defined as the movement of a cell on a surface in the direction of the long axis of the cell. because this definition is operational and not mechanistic, the underlying molecular motor(s) may be quite different in diverse microbes. in fact, studies ...199910477310
diagnosis of kingella kingae arthritis by polymerase chain reaction analysis. 199910530485
kingella kingae prosthetic valve endocarditis. 199910547947
use of blood culture systems for isolation of kingella kingae from synovial fluid. 199910610378
isolation of pantoea agglomerans in two cases of septic monoarthritis after plant thorn and wood sliver injuries.arthritis after plant injury is often apparently aseptic. we report two cases due to pantoea agglomerans. in one case, the bacterium was isolated only from the pediatric blood culture media, bactec peds plus, monitored in bactec 9240, and not from the other media inoculated with the joint fluid. this procedure could help improve the diagnosis of septic arthritis.200010618144
population-based study of invasive kingella kingae infections. 200010653579
kingella kingae infections in children.to increase awareness of kingella kingae infections in children by presenting four cases seen at the gold coast hospital, southport, queensland, and reviewing the literature.200010723701
importance of kingella kingae as a pediatric pathogen in the united states. 200010749477
kingella endocarditis and meningitis in a patient with sle and associated antiphospholipid syndrome.we describe a patient with sle and antiphospholipid syndrome who presented with severe headache and fever. lumbar puncture analyses indicated meningitis. kingella kingae was isolated from her blood cultures. a large mobile vegetation was seen on her mitral valve. the association between sle, libman-sacks endocarditis and bacterial endocarditis is discussed.200010878736
[septic arthritis in two young children caused by unusual gram-negative pathogens].two children, a girl aged 2 years and a boy aged 10 months, were moderately ill with signs of inflammation of the left and the right knee, respectively. both had had pharyngitis, and the boy also had paronychia of the right foot. the gram preparation of synovial fluid showed gram-positive cocci in the girl, while kingella kingae was cultured. in the boy, a moraxella was cultured from the synovial fluid using an aerobic blood culture system. both recovered without sequelae after adequate antibiot ...200010941437
isolation of kingella kingae from synovial fluids using four commercial blood culture bottles.according to the literature, kingella kingae may be an underdiagnosed cause of joint and bone infections in children. the use of the bactec blood culture system for culture of joint fluids has dramatically improved the isolation of this fastidious bacterium. the aim of this study was to test the recovery rate and detection time of four commercial blood culture systems: three different bact/alert (organon teknika, usa) bottles and one bactec (becton dickinson microbiology systems, usa) bottle, al ...200011014623
[kingella kingae osteoarticular infections in children. a report of a series of eight new cases].kingella kingae is a gram-negative bacillus which belongs to the neisseriaceae family. its involvement in osteoarticular infections is relatively recent.200011028199
nonspecific adherence by actinobacillus actinomycetemcomitans requires genes widespread in bacteria and archaea.the gram-negative coccobacillus, actinobacillus actinomycetemcomitans, is the putative agent for localized juvenile periodontitis, a particularly destructive form of periodontal disease in adolescents. this bacterium has also been isolated from a variety of other infections, notably endocarditis. fresh clinical isolates of a. actinomycetemcomitans form tenacious biofilms, a property likely to be critical for colonization of teeth and other surfaces. here we report the identification of a locus o ...200011029439
[kingella kingae in a 10-year-old girl]. 200011109731
a rare manifestation of kingella kingae infection.kingella kingae is an aerobic gram-negative coccobacillus that has been associated predominantly with bone and joint infection but also with septicemia and endocarditis. until now, only four cases of proven k. kingae meningitis have been reported. we describe a case of a k. kingae meningitis in a male adolescent who presented with a history of fever of unclear origin.200011113660
global aphasia without hemiparesis secondary to kingella kingae endocarditis. 200011115245
endocarditis due to rare and fastidious bacteria.the etiologic diagnosis of infective endocarditis is easily made in the presence of continuous bacteremia with gram-positive cocci. however, the blood culture may contain a bacterium rarely associated with endocarditis, such as lactobacillus spp., klebsiella spp., or nontoxigenic corynebacterium, salmonella, gemella, campylobacter, aeromonas, yersinia, nocardia, pasteurella, listeria, or erysipelothrix spp., that requires further investigation to establish the relationship with endocarditis, or ...200111148009
antibiotic susceptibility of kingella kingae isolates from respiratory carriers and patients with invasive infections.the antimicrobial drug susceptibilities of 145 isolates of kingella kingae to eight antibiotics were determined by the disc diffusion method. in addition, penicillin mics were determined by the etest. study isolates included 37 from blood, 34 from the skeletal system and 74 from respiratory carriers. all isolates were beta-lactamase negative and susceptible to erythromycin, gentamicin, chloramphenicol, tetracycline and ciprofloxacin. a single isolate exhibited resistance to trimethoprim-sulphame ...200111157905
use of bactec 9240 blood culture system for detection of brucella melitensis in synovial fluid.synovial fluid specimens aspirated from patients with arthritis were inoculated into an aerobic peds plus blood culture bottle and monitored by the bactec 9240 instrument for 4 weeks. a total of 1,072 synovial fluid cultures were processed, and 15 (0.14%) were positive for brucella melitensis. a single culture, harboring 1.3 cfu of viable organisms per ml, was missed by the instrument. all 14 positive bactec cultures were detected within 3 to 7 days.200111158139
two cases of diskitis attributable to anaerobic bacteria in children.diskitis, an inflammation of the intervertebral disk, is generally attributable to staphylococcus aureus and rarely staphylococcus epidermidis, kingella kingae, enterobacteriaciae, and streptococcus pneumoniae. in many cases, no bacterial growth is obtained from infected intervertebral discs. although anaerobic bacteria were recovered from adults with spondylodiscitis, these organisms were not reported before from children. the recovery of anaerobic bacteria in 2 children with diskitis is report ...200111158500
diagnostics of neisseriaceae and moraxellaceae by ribosomal dna sequencing: ribosomal differentiation of medical microorganisms.fast and reliable identification of microbial isolates is a fundamental goal of clinical microbiology. however, in the case of some fastidious gram-negative bacterial species, classical phenotype identification based on either metabolic, enzymatic, or serological methods is difficult, time-consuming, and/or inadequate. 16s or 23s ribosomal dna (rdna) bacterial sequencing will most often result in accurate speciation of isolates. therefore, the objective of this study was to find a hypervariable ...200111230407
[endocarditis due to hacek bacteria. a case report of endocarditis due to kingella kingae].endocarditis is a common disease in hospital practice. identification of the microorganism responsible for the valvular damage is essential to establish the prognosis and to determine the optimal antibiotic treatment. in some cases of endocarditis the diagnosis is laborious, especially when the responsible microorganism is difficult to detect using standard culture techniques. here we report a case of native aortic valve endocarditis due to kingella kingae, a gram negative organism of the hacek ...200111234709
septic arthritis due to kingella kingae in an adult. 200111235790
kingella kingae endocarditis in a sixteen-month-old-child.we report the case of a child with acute neurologic symptoms who was found to have bacterial endocarditis caused by kingella kingae. the case alerts microbiologists and pediatricians to an organism that has rarely been reported to cause endocarditis in children.200111332678
bacterial diversity in human subgingival plaque.the purpose of this study was to determine the bacterial diversity in the human subgingival plaque by using culture-independent molecular methods as part of an ongoing effort to obtain full 16s rrna sequences for all cultivable and not-yet-cultivated species of human oral bacteria. subgingival plaque was analyzed from healthy subjects and subjects with refractory periodontitis, adult periodontitis, human immunodeficiency virus periodontitis, and acute necrotizing ulcerative gingivitis. 16s ribos ...200111371542
soft tissue infection caused by kingella kingae in a child.during the last years an increasing number of reports concerning kingella kingae infections in children has been published. most cases were osteoarticular infections. the authors report the clinical and laboratory findings from a 3-year-old child with a presternal soft tissue infection due to k kingae. after surgical excochleation and antibiotic treatment there was an uneventful recovery. j pediatr surg 36:946-947.200111381434
optimisation of the prevention and treatment of bacterial endocarditis.this paper reviews currently established guidelines for the prevention and treatment of bacterial endocarditis. endocarditis remains a life-threatening disease with substantial morbidity and mortality. primary prevention of endocarditis, whenever possible, is therefore very important. in an individual with endocarditis, rapid diagnosis and effective treatment are essential to good patient outcome. the guidelines discussed here are largely based on those issued by the american heart association. ...200111419916
production of bartonella genus-specific monoclonal antibodies.monoclonal antibodies (mabs) which react with heat-resistant proteins with molecular masses of 32 to 33 kda of 14 different bartonella species were produced. these antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except mab b3d4, which reacted with chlamydia psittaci and chlamydia trachomatis at low titers. the identification of a common bartonella antigenic protein will make it possible to later produce a diagnostic antigen by cloning and e ...200111427441
whipple's disease and "tropheryma whippelii".whipple's disease is a rare bacterial infection that may involve any organ system in the body. it occurs primarily in caucasian males older than 40 years. the gastrointestinal tract is the most frequently involved organ, with manifestations such as abdominal pain, malabsorption syndrome with diarrhea, and weight loss. other signs include low-grade fever, lymphadenopathy, skin hyperpigmentation, endocarditis, pleuritis, seronegative arthritis, uveitis, spondylodiscitis, and neurological manifesta ...200111432814
structural and genetic analyses of o polysaccharide from actinobacillus actinomycetemcomitans serotype f.the oral bacterium actinobacillus actinomycetemcomitans is implicated as a causative agent of localized juvenile periodontitis (ljp). a. actinomycetemcomitans is classified into five serotypes (a to e) corresponding to five structurally and antigenically distinct o polysaccharide (o-ps) components of their respective lipopolysaccharide molecules. serotype b has been reported to be the dominant serotype isolated from ljp patients. we determined the lipopolysaccharide o-ps structure from a. actino ...200111500407
[kingella kingae osteomyelitis].a case of kingella kingae osteomyelitis in a 1-year-old child is described. kingella kingae osteoarticular infections in children and the difficulties of isolating this slow growing, fastidious bacterium are discussed.200111723690
culture with bactec peds plus/f bottle compared with conventional methods for detection of bacteria in synovial fluid.an evaluation was undertaken to determine the utility of the bactec peds plus/f bottle and the bactec 9240 instrument (becton dickinson diagnostic instrument systems, sparks, md.) for the detection of clinically significant microorganisms in synovial fluid specimens. the peds plus/f bottle was used because in our laboratory the quantity of synovial fluid available for culture is frequently in the range of 0.5 to 3.0 ml. the culture results obtained with the peds plus/f bottle were compared to th ...200111724863
recurrent soft tissue abscesses caused by legionella cincinnatiensis.recurrent soft tissue abscesses of the jaw, wrist, and arm developed in a 73-year-old housewife with nephrotic syndrome and immunoglobulin a(kappa) gammopathy of unknown etiology. conventional cultures remained negative, despite visible gram-negative rods on microscopy. broad-spectrum pcr revealed legionella cincinnatiensis, which was confirmed by isolation of the organism on special legionella medium. infections due to legionella species outside the lungs are rare. l. cincinnatiensis has been i ...200111724886
bilateral endogenous endophthalmitis caused by hacek microorganism.to report a case of bilateral endogenous endophthalmitis caused by a hacek group organism (actinobacillus actinomycetemcomitans).200211755854
[kingella kingae: occult bacteriemia in a child]. 200211827663
[kingella kingae endocarditis]. 200111852509
[kingella kingae: a rare cause of meningitis].kingella kingae is the second most frequent germ involved in arthritis affecting young children. this germ isolation on ordinary environment is difficult, which may explain why it is seldom known. it is now widely accepted that a direct inoculate of articular and osseous samples on liquid substrate improves the culture sensitivity. other septic localizations have been described such as endocarditis or, less commonly, meningitis. case report: we report the observation of a five-year-old child, tr ...200211865547
nontuberculous spondylodiscitis in children.forty-two children with nontuberculous spondylodiscitis treated between 1966 and 1997 were reviewed, and the clinical, paraclinical, and therapeutic results are presented. the study shows the difficulties of diagnosis and understanding the pathophysiology of the disease. additional information is provided by new imaging techniques, disc aspiration, and biopsy. the mean age at treatment was 4 years 6 months. the initial clinical presentation was often misleading and the diagnosis was often delaye ...200211961447
comparative evaluation of the bd phoenix and vitek 2 automated instruments for identification of isolates of the burkholderia cepacia complex.we evaluated two new automated identification systems, the bd phoenix (becton dickinson) and the vitek 2 (biomérieux), for identification of isolates of the burkholderia cepacia complex (bcc). the test sample included 42 isolates of the highly virulent and epidemic genomovar iii, 45 isolates of b. multivorans, and 47 isolates of other members of the bcc. rates of correct identification by the bd phoenix and vitek 2 were similar when all bcc isolates were considered (50 and 53%, respectively) but ...200211980954
combined antibiotic and free radical trap treatment is effective at combating staphylococcus-aureus-induced septic arthritis.although early antibiotic treatment of patients with septic arthritis eradicates bacteria, joint destruction commonly results from the unregulated host inflammatory responses to infection. the spin trap compound phenyl-n-tert-butyl nitrone (pbn) has been shown to have both anti-inflammatory and antioxidant effects. the aim of this study was to assess the effect of combined systemic administration of pbn and cloxacillin on the development of staphylococcus aureus arthritis.three days after naval ...200212010570
mortality from infective endocarditis: clinical predictors of outcome.to identify clinical markers available within the first 48 hours of admission that are associated with poor outcome in infective endocarditis.200212067945
acute septic arthritis.acute septic arthritis may develop as a result of hematogenous seeding, direct introduction, or extension from a contiguous focus of infection. the pathogenesis of acute septic arthritis is multifactorial and depends on the interaction of the host immune response and the adherence factors, toxins, and immunoavoidance strategies of the invading pathogen. neisseria gonorrhoeae and staphylococcus aureus are used in discussing the host-pathogen interaction in the pathogenesis of acute septic arthrit ...200212364368
epidemiological features of invasive kingella kingae infections and respiratory carriage of the organism.the age, sex, and seasonal distributions of invasive kingella kingae infections in southern israel were examined and compared to the epidemiology of respiratory carriage of the organism. medical records of all patients diagnosed between 1988 and 2002 were reviewed, and 2,044 oropharyngeal specimens were cultured on selective media during two periods (february to may and october to december) in 2001. invasive infections significantly affected children (73 of 74 patients [98.6%] were younger than ...200212409394
review article: paediatric bone and joint infection.paediatric musculoskeletal infection remains an important cause of morbidity. methicillin sensitive staphylococcus aureus is still the most common organism although the incidence of methicillin resistant s. aureus in the community is rising. osteomyelitis and septic arthritis due to haemophilus influenzae is decreasing in incidence secondary to immunisation and in some units has been replaced by infections with the gram negative bacillus, kingella kingae. recent prospective studies indicate that ...200112468850
molecular diagnosis of infective endocarditis by pcr amplification and direct sequencing of dna from valve tissue.we used broad-range eubacterial pcr amplification followed by direct sequencing to identify microbial pathogens in heart valve material from 29 patients with histologically confirmed infective endocarditis and 23 patients free of infective endocarditis. microorganisms cultured by conventional techniques matched those identified by pcr in 21 cases. pcr alone identified the causative agent in three cases (streptococcus bovis, staphylococcus cohnii, and coxiella burnetii), allowing better patient m ...200312574279
molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients.to assess the clinical validity of polymerase chain reaction (pcr) based molecular methods in the microbiological diagnosis of culture negative infective endocarditis in a group of surgically treated patients.200312591825
low penetrance, broad resistance, and favorable outcome of interleukin 12 receptor beta1 deficiency: medical and immunological implications.the clinical phenotype of interleukin 12 receptor beta1 chain (il-12rbeta1) deficiency and the function of human il-12 in host defense remain largely unknown, due to the small number of patients reported. we now report 41 patients with complete il-12rbeta1 deficiency from 17 countries. the only opportunistic infections observed, in 34 patients, were of childhood onset and caused by weakly virulent salmonella or mycobacteria (bacille calmette-guérin -bcg- and environmental mycobacteria). three pa ...200312591909
destructive osteomyelitis of the fibular epiphysis due to kingella kingae. 200312674914
innocent victim of a localised outbreak: legionella endocarditis.legionella pneumophila endocarditis is extremely rare. the case of a fit 26 year old man who had previously undergone homograft aortic root replacement is reported. he was admitted with legionella pneumonia during the recent localised outbreak but went on to develop endocarditis. his aortic valve was replaced with a mechanical valve and he made an uneventful recovery. public health issues and diagnosis in susceptible patients during localised outbreaks are discussed.200312695481
three cases of kingella kingae infection in young children. 200312739137
immune response to invasive kingella kingae infections, age-related incidence of disease, and levels of antibody to outer-membrane proteins.the immune response to kingella kingae was determined by enzyme-linked immunosorbent assay, using outer-membrane proteins as coating antigen, in 19 children with invasive infection. the age-related incidence of k. kingae disease in southern israel during 1988-2002 was calculated and correlated with serum antibody levels in healthy children. significant increases in immunoglobulin g (igg) levels were found in children convalescing after invasive infections. the incidence was 1.3, 40.3, 23.9, 5.7, ...200312905136
osteoarticular infections caused by kingella kingae in children: contribution of polymerase chain reaction to the microbiologic diagnosis. 200314515832
an adult case of oral infection with kingella kingae.an exceptional case of microbiologically confirmed oral infection with kingella kingae in an immunocompetent adult (30-year-old woman) is presented and the pathogenesis is discussed and related to known literature data.k. kingae is a rather common but yet relatively unknown commensal corroding bacterium from the oro- and nasopharynx in healthy children, which might turn into a human pathogen causing osteomyelitis, arthritis, spondylitis, endocarditis and intervertebral diskitis in young children ...200414690666
characterization of haemophilus segnis, an important cause of bacteremia, by 16s rrna gene sequencing.we describe the application of 16s rrna gene sequencing in defining eight cases of bacteremia due to haemophilus species other than haemophilus influenzae (non-h. influenzae bacteremia) during a 7-year period. the first case of acute pyelonephritis due to haemophilus segnis is also reported. in contrast to the extremely rare incidence of h. segnis infections reported previously, our results suggested that h. segnis is an important cause of non-h. influenzae bacteremia.200414766878
[kingella kingae and osteoarticular infections in children].in septic arthritis and osteomyelitis in children, the infectious agent is frequently not identified because of failure in obtaining bacterial growth from blood culture or osteoarticular aspirations.200414983196
real-time multiplex pcr assay for detection of brucella spp., b. abortus, and b. melitensis.the identification of brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. here, we describe a real-time pcr assay for confirmation of presumptive brucella isolates. the assay was designed in a multiplex format that will allow the rapid identification of brucella spp., b. abortus, and b. melitensis in a single test.200415004098
[investigation of the bacteria in the seawater of xisha in the south china sea and their antibiotic sensitivity profile].to investigate the distribution and antibiotic sensitivity of the bacteria in the seawater of xisha in the south china sea.200415041561
osteomyelitis/septic arthritis caused by kingella kingae among day care attendees--minnesota, 2003.kingella kingae is a fastidious gram-negative coccobacillus that colonizes the respiratory and oropharyngeal tract in children. k. kingae occasionally causes invasive disease, primarily osteomyelitis/septic arthritis in young children, bacteremia in infants, and endocarditis in school-aged children and adults. although diagnosis of this organism frequently is missed, invasive disease is uncommon. only sporadic, non-epidemiologically linked cases have been reported previously. in october 2003, th ...200415041952
kingella kingae infections in children--united states, june 2001-november 2002.kingella kingae is recognized increasingly as a cause of skeletal infections in children. recent studies indicate that direct inoculation of clinical specimens into aerobic blood culture bottles (abcbs), instead of direct plating of specimens on solid media, might improve recovery of the fastidious bacteria. prompted by a report of a possible cluster of osteoarticular infections caused by k. kingae among children, the infectious diseases society of america emerging infections network (idsa-ein) ...200415041953
[discitis caused by kingella kingae--case report].the patient with acute infection and the presence of bacterium kingella kingae cultured from cerebrospinal fluid is presented. the analysis of clinical picture and the results of all performed tests did not allow to diagnose cerebrospinal meningitis. despite of being not sure if kingella kingae was an etiopathogenetic factor in the described subject, we decided to discuss this interesting case.200315049217
endocarditis due to kingella kingae: a patient report. 200415094954
diagnostic criteria and problems in infective endocarditis. 200415145855
indications and optimal timing for surgery in infective endocarditis. 200415145858
multi-pathogens sequence containing plasmids as positive controls for universal detection of potential agents of bioterrorism.the limited circulation of many of the agents that are likely to be used in a bioterrorism attack precludes the ready availability of positive controls. this means that only specialized laboratories can screen for the presence of these agents by nucleic amplification assays. calibrated controls are also necessary for quantitative measurements. primers and probes to be used in both conventional and real-time pcr assays were designed for the detection of agents likely to be used by a bioterrorist. ...200415147587
kingella kingae: from medical rarity to an emerging paediatric pathogen.in recent years, kingella kingae has emerged as an important cause of invasive infections in young children, especially septic arthritis, osteomyelitis, spondylodiscitis, bacteraemia, and endocarditis, and less frequently lower respiratory tract infections and meningitis. the organism is part of the pharyngeal flora of young children and is transmitted from child-to-child. the clinical presentation of invasive k kingae disease is often subtle and laboratory tests are frequently normal. a substan ...200415172344
henoch-schonlein purpura associated with kingella kingae bacteremia. 200415174803
emerging issues in infective endocarditis.infective endocarditis, a serious infection of the endocardium of the heart, particularly the heart valves, is associated with a high degree of illness and death. it generally occurs in patients with altered and abnormal heart architecture, in combination with exposure to bacteria through trauma and other potentially high-risk activities involving transient bacteremia. knowledge about the origins of endocarditis stems from the work of fernel in the early 1500s, and yet this infection still prese ...200415207065
unsuspected kingella kingae infections in afebrile children with mild skeletal symptoms: the importance of blood cultures. 200415241689
[septic arthritis due to kingella kingae: difficulties of diagnosis]. 200415274891
kingella kingae endocarditis and sepsis in an infant. 200415300433
infective endocarditis: a review of the best treatment options.despite significant advances in antimicrobial therapy and an enhanced ability to diagnose and treat complications, infective endocarditis is still associated with substantial morbidity and mortality today, and its incidence has not decreased over the past decades. this apparent paradox may be explained by a progressive change in risk factors, leading to an evolution in its epidemiological and clinical features. in fact, new risk factors for endocarditis have emerged, such as intravenous drug abu ...200415330728
enhanced culture detection of kingella kingae, a pathogen of increasing clinical importance in pediatrics.osteoarticular infection and occult bacteremia are the 2 invasive infectious pathologies most frequently associated in childhood with kingella kingae. we report a series of 11 patients in whom osteomyelitis predominates over septic arthritis, which is the reverse of other series, probably as a consequence of inoculation of samples during surgery on agar media, used in combination with or as an alternative to inoculation into blood culture bottles. although k. kingae infections usually follow a b ...200415361737
kingella kingae infections of the skeletal system in children: diagnosis and therapy.as the result of improved bacteriological techniques, kingella kingae is emerging as an important cause of infections of the skeletal system in children younger than 2 years of age. this review details the bacteriological features and detection methods of this pathogen, as well as the epidemiology, clinical presentation, treatment and prognosis of septic arthritis, osteomyelitis and diskitis caused by the organism.200415482240
characterization and immunogenicity of kingella kingae outer-membrane proteins.in recent years, kingella kingae has emerged as an important pediatric pathogen but the antigenicity of the organism and the host immune response have not been studied. outer membrane proteins (omps) of 57 k. kingae isolates were characterized and the immune response of 19 children with invasive infections was studied by immunoblotting. kingella kingae omps were remarkably similar disregarding place and time of isolation and associated clinical condition (asymptomatic carriage, bacteremia, endoc ...200515607635
[acute osteomyelitis and septic arthritis in children: one year experience].to describe bacteriologic epidemiology of bone and joint infections, a total of 52 osteomyelitis, 52 arthritis and 20 osteoarthritis of children aged one month to 15 years during a one-year period (2001) were included in a retrospective unicentric review. the mean age was 3,9 +/-3,6 years. fever and pain were the most common clinical symptoms. the site of infection was single in 95%, involving lower extremities in 80%. bone scintigraphy was abnormal in 71% of osteomyelitis. positive cultures was ...200515653049
acute osteomyelitis and septic arthritis in children.to review the clinical presentation, clinical management and organisms responsible for acute haematogenous osteomyelitis (aho) and septic arthritis (sa) in the post haemophilus influenzae type b (hib) vaccine era and to evaluate current australian antibiotic guidelines for these conditions.200515670227
[osteoarticular infection in children: evaluation of a diagnostic and management protocol].the purpose of this study was to evaluate a diagnostic and management protocol for osteoarticular infection in children which was developed at the rouen university hospital in 1993. we studied the technical aspects of the protocol allowing improved certain diagnosis and bacteriological documentation as well as the reduction in intravenous treatments and hospital stay.200415711488
bacterial zoonoses and infective endocarditis, algeria.blood culture-negative endocarditis is common in algeria. we describe the etiology of infective endocarditis in this country. samples from 110 cases in 108 patients were collected in algiers. blood cultures were performed in algeria. serologic and molecular analysis of valves was performed in france. infective endocarditis was classified as definite in 77 cases and possible in 33. causative agents were detected by blood cultures in 48 cases. all 62 blood culture-negative endocarditis cases were ...200515752438
the actinobacillus actinomycetemcomitans autotransporter adhesin aae exhibits specificity for buccal epithelial cells from humans and old world primates.cells of the gram-negative periodontopathogen actinobacillus actinomycetemcomitans express a surface-exposed, outer membrane autotransporter protein, designated aae, which has been implicated in epithelial cell binding. we constructed a mutant strain of a. actinomycetemcomitans that contained a transposon insertion in the aae structural gene (aae) and tested the mutant to determine its ability to bind to buccal epithelial cells (becs) isolated from healthy volunteers. significantly fewer mutant ...200515784534
prosthetic valve endocarditis: who needs surgery? a multicentre study of 104 cases.to identify the prognostic markers of a bad outcome in a large population of 104 patients with prosthetic valve endocarditis (pve), and to study the influence of medical versus surgical strategy on outcome in pve and thus to identify patients for whom surgery may be beneficial.200515958370
outbreak of osteomyelitis/septic arthritis caused by kingella kingae among child care center attendees.kingella kingae often colonizes the oropharyngeal and respiratory tracts of children but infrequently causes invasive disease. in mid-october 2003, 2 confirmed and 1 probable case of k kingae osteomyelitis/septic arthritis occurred among children in the same 16- to 24-month-old toddler classroom of a child care center. the objective of this study was to investigate the epidemiology of k kingae colonization and invasive disease among child care attendees.200516024681
[eikenella corrodens infective endocarditis].the hacek microorganisms (haemophilus spp, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae) account for 3% of the cases of endocarditis. they have the following similar clinical and microbiological properties: are gram-negative bacilli, more easily isolated in aerobic media; their cultures require prolonged incubation time for growing (mean, 3.3 days); and may be considered part of normal flora of upper respiratory tract and oropharynx. the ...200516041457
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