Publications

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gliding motility in the organisms bacterium anitratum (b5w), moraxella lwoffi and alkaligenes haemolysans, as compared to moraxella nonliquefaciens. 196314083764
reciprocal genetic transformation between neisseria catarrhalis and moraxella nonliquefaciens. 196414250800
studies on transformation in moraxella. and organisms assumed to be related to moraxella 6. a distinct group of moraxella nonliquefaciens-like organisms (the "19116/51" group). 19655177528
studies on transformation in moraxella and organisms assumed to be related to moraxella. 5. streptomycin resistance transformation between serum-liquefying, nonhaemolytic moraxellae, moraxella bovis and moraxella nonliquefaciens. 19655884703
studies on transformation in moraxella and organisms assumed to be related to moraxella. 3. quantitative streptomycin resistance transformation between moraxella bovis and moraxella nonliquefaciens strains. 196514276527
corroding and spreading colonies in moraxella nonliquefaciens. 19695823363
correlation between the fimbriated state and competence of genetic transformation in moraxella nonliquefaciens strains. 19705274411
electron microscopical and serological characteristics associated with colony type in moraxella nonliquefaciens. 19705278895
selective medium for moraxella nonliquefaciens.a medium is described which selectively inhibits most bacteria from the normal flora of the conjunctiva, the lid-margin, and the upper respiratory tract but permits growth of moraxella nonliquefaciens, m. liquefaciens, and the less fastidious strains of m. lacunata. the selective conditions are obtained with thiostrepton, tyrothricin, and sodium nalidixinate as inhibitors. incubation under increased co(2) pressure increased the number of primary isolations. m. nonliquefaciens was isolated on the ...19705437302
bovine infectious keratoconjunctivitis: serological aspects of moraxella bovis infection.a modification of a gel diffusion precipitin test (gdpt) was used to detect antibodies for moraxella bovis (m. bovis) in the sera of cattle affected with bovine infectious keratoconjunctivitis (bik). the test was also used for the detection of sequential antibody development in cattle vaccinated with cultures of m. bovis. also, strains of m. bovis isolated from cattle herds affected with bik were characterized serologically as a part of an identification scheme using the test.a comparison of the ...19714253466
studies on bacterial surface translocation. 2. correlation of twitching motility and fimbriation in colony variants of moraxella nonliquefaciens, m. bovis, and m. kingii. 19724505772
the problem of pathogenicity of moraxellae in the urogenital tract of women.the incidence of moraxella nonliquefaciens an m. liquefaciens in the urogenital tract is relatively low. these microorganisms were detected in 0.77% of cases during the examination of a total of 3 761 women. m. nonliquefaciens was found in 26 out of 29 cases. the frequency of occurrence did not differ in the groups of healthy women, of women with inflammatory signs in the urogenital tract and of women with confirmed gonorrhoea. we therefore believe that these moraxellae do not play any pathogene ...19751237516
pneumonitis and pulmonary abscess associated with moraxella nonliquefaciens.a 78-year-old man with pneumonitis and pulmonary abscess associated with moraxella nonliquefaciens is presented. this organism was found by culture of both transtracheal aspirate and sputum. no previous reports have associated m nonliquefaciens with infection of the lower respiratory tract, although sinusitis and bronchitis have been reported. possible predisposing factors in our patient included carcinoma of the larynx, as well as alcohol ingestion and cigarette smoking.1976975988
purification and n-terminal sequence of a fimbrial protein from moraxella nonliquefaciens. 1977838045
in vitro antibiotic sensitivity of moraxella species.minimal inhibitory concentrations of 17 antibacterial agents for 34 moraxella strains were determined using a plate dilution method. a strain of moraxella nonliquefaciens was found which produced beta-lactamase and was resistant to ampicillin and carbenicillin but not to cephalothin. several strains were relatively resistant to erythromycin and sulfisoxazole. disk sensitivity tests could be used to reliably predict penicillin and erythromycin resistance but not sulfisoxazole resistance.1978699676
antigenic characterisitcs of moraxella nonliquefaciens fimbriae in double immunodiffusion.rabbit antisera against purified fimbriae (pili) from moraxella nonliquefaciens detected three fimbrial antigenic components, one or two of which appeared to be present in other fimbriated strains of m. nonliquefaciens and the closely related m. bovis. maximal precipitation with the antisera required some denaturation of the antigen. ultrasonication, repeated freeze-thawing, heating, and agents like kbr, nascn and urea were effective in liberating the antigen in diffusible forms. the morphology ...197899975
density gradient centrifugation in urografin of moraxella and kingella cells and appendages.purification of fimbriae (pili) by density gradient banding in urografin medium was attempted. moraxella nonliquefaciens and kingella kingae fimbriae were of higher density than their cells of origin, but fimbrial fractions obtained by homogenization and differential centrifugation still banded together with presumed outer membrane fragments and some whole cells in urografin gradients. the cellular density of genetic variants with different fimbriation/competence levels was also studied. for one ...1978358743
endocarditis caused by moraxella nonliquefaciens.we have described a case of endocarditis caused by moraxella nonliquefaciens on a prosthetic hancock valve, which was cured with a six-week course of ampicillin and gentamicin therapy. cases of moraxella septicemia or endocarditis are uncommon, and this apparently represents the first case of moraxella nonliquefaciens endocarditis on a prosthetic valve. the fastidious growth characteristics of this and similar species may require prolonged incubation of blood cultures and development of differen ...1979505092
isolation of a relatively pure outer membrane fraction of moraxella nonliquefaciens and a comparison of its characteristics with the cytoplasmic membrane-containing material.cell envelope fractions of moraxella nonliquefaciens were isolated by a slight modification of osborn's method. two main membrane fractions were characterized chemically and morphologically. the density of the fraction containing cytoplasmic membrane material was 1.17 to 1.18 g cm-3 compared with 1.24 to 1.27 g cm-3 for the outer membrane fraction. lipopolysaccharide was detected almost exclusively in the outer membrane fraction and sodium dodecyl sulphate polyacrylamide gel electrophoresis of t ...19807365451
endophthalmitis caused by moraxella nonliquefaciens.we report the first case of endophthalmitis caused by moraxella nonliquefaciens. most likely, entry followed minor trauma from a contact lens, with the marked invasiveness of the organism resulting from the patient's immunocompromised state. this case, in addition, serves to illustrate the advantage of genetic transformation assay to differentiate the species of this group of organisms.19827072642
[neonatal meningitis due to moraxella nonliquefaciens]. 19827170520
studies on outer membrane proteins of moraxella nonliquefaciens.outer membrane fractions of moraxella nonliquefaciens 7784 strains sc-c and n-b, isolated by extraction with lithium acetate, were analysed by sds-page. three main proteins were found, of which one, with an apparent molecular weight of 19500, was undetectable in membranes isolated by lysis of lysozyme/edta spheroplasts. all three major proteins were heat modifiable.19836875514
septicemia due to moraxella nonliquefaciens in a patient with multiple myeloma. 19836879119
surface polysaccharide of moraxella non-liquefaciens identical to neisseria meningitidis group b capsular polysaccharide. a chemical and immunological investigation.in whole cell preparations of 27 nonmucoid strains of moraxella nonliquefaciens neuraminic acid was detected by gas chromatography (gc) in 16 (59%) of the strains. seven neuraminic-acid-containing strains were tested for agglutination with diagnostic group-specific meningococcal antisera produced in rabbits, and all were positive with group b serum. counter-immunoelectrophoresis of bacterial suspensions of the three strains with the strongest reaction with such anti-group b serum gave distinct p ...19836413906
general approach to bacterial nutrition: growth factor requirements of moraxella nonliquefaciens.a general procedure was devised for the determination of growth factor requirements of heterotrophic bacteria based upon identification of individual nutrients as they are successively depleted from a limited quantity of complex medium. by using this approach, it was possible to develop a defined medium for growth of moraxella nonliquefaciens that contained nine amino acids and three vitamins. three of the amino acids, proline, serine, and cysteine, were required in unusually high concentrations ...19846438065
intrageneric and intergeneric transfer of branhamella catarrhalis beta-lactamase production.intrageneric and intergeneric conjugational transfer of beta-lactamase production to a branhamella catarrhalis recipient is described. the branhamella donor strains were fresh isolates subcultured onto penicillin-containing media directly from the primary plates. a beta-lactamase positive moraxella nonliquefaciens served as donor in the intergeneric matings. the substrate profiles of the branhamella and moraxella enzymes were indistinguishable as determined by biological assays. excessive endonu ...19846610925
[sepsis caused by moraxella nonliquefaciens]. 19853981909
moraxella nonliquefaciens endophthalmitis after trabeculectomy.endophthalmitis caused by moraxella nonliquefaciens developed five years after trabeculectomy in a 67-year-old man and 15 months after trabeculectomy in a 62-year-old women. symptoms in both cases included pain, inflammation, and decreased visual acuity. aggressive treatment with antibiotics and corticosteroids led to resolution within a few days.19853976811
characterization of the plasmid-mediated beta-lactamase in branhamella catarrhalis, with special reference to substrate affinity.the plasmid-mediated branhamella catarrhalis beta-lactamase bro-1, also found in moraxella nonliquefaciens, was characterized as regards substrate profile, isoelectric point and relative substrate affinity index (rsai) to various substrates and compared in these aspects with the tem-1 enzyme of haemophilus influenzae. as measured by a biological assay and with high performance liquid chromatography (hplc), bro-1 was found to hydrolyse carbenicillin, mecillinam, methicillin and cefaclor with a hi ...19853872294
beta-lactamase production in the upper respiratory tract flora.in order to determine the recovery rate of species of the genera haemophilus and moraxella (including subgenus branhamella) from the upper respiratory tract and the incidence of beta-lactamase production within these genera, cultures were made of nose and throat swab specimens and adenoid tissue in 50 children undergoing adenoidectomy. haemophilus influenzae was isolated from 92% of the children. all children harboured strains of haemophilus spp. and in 46%, at least one strain produced the tem- ...19863490974
plasmid-mediated beta-lactamase in branhamella catarrhalis.the plasmid-mediated beta-lactamase in branhamella catarrhalis (bro-1), also occurring in moraxella nonliquefaciens, differs from other known plasmid-mediated beta-lactamases in gram-negative bacteria regarding substrate profile and isoelectric point. b. catarrhalis strains previously reported to produce beta-lactamases deviating from bro-1 were tested, and the beta-lactamases did not differ significantly from bro-1 in substrate profile, isoelectric point or relative substrate affinity index (rs ...19863488198
upper respiratory tract infections. ecological and therapeutic aspects of beta-lactamase production with special reference to branhamella catarrhalis.available data indicate that the most common beta-lactamase produced by branhamella catarrhalis is plasmid mediated. the same enzyme occurs in moraxella nonliquefaciens, a commensal in the upper respiratory tract. the ability to produce the enzyme, which is known as bro-1, can be transferred by conjugation from m. nonliquefaciens to b. catarrhalis. since the first beta-lactamase-producing strains of b. catarrhalis appeared in 1977, the frequency of beta-lactamase production has increased rapidly ...19863488190
combined genetic transformation and nutritional assay for identification of moraxella nonliquefaciens.a combined genetic transformation and nutritional assay is described that permits definitive identification of clinically isolated strains of moraxella nonliquefaciens. crude dna preparations of strains of various moraxella species were used to transform nutritional mutants of a stably competent strain of m. nonliquefaciens for ability to grow on a defined medium (mn-b). dna samples from 24 independently isolated strains of m. nonliquefaciens all resulted in massive (4+) transformation of each o ...19873654942
taxonomy of pasteurella anatipestifer. 1. dna base composition and dna-dna hybridization analysis.dna was isolated from 15 strains of pasteurella anatipestifer and from one strain each of moraxella nonliquefaciens, m. bovis, pasteurella multocida, p. haemolytica, p. gallinarum, p. pneumotropica, and p. ureae. the guanine-plus-cytosine contents of p. anatipestifer ranged from 32 to 35 mole %, whereas those of moraxella and pasteurella spp. were much higher, ranging from 40 to 45 mole %. dna-dna hybridization analysis revealed that homology of nine p. anatipestifer strains to strains atcc 1184 ...19873579792
murine monoclonal antibodies for detection of antigens and culture identification of neisseria meningitidis group b and escherichia coli k-1.four murine monoclonal antibodies which reacted with a (2----8)alpha-linked sialic acid polysaccharide were produced. three of the antibodies reacted specifically with neisseria meningitidis serogroup b and escherichia coli k-1 polysaccharide antigens, whereas one antibody cross-reacted with n. meningitidis group c polysaccharide antigen, a (2----9)alpha-linked homopolymer of sialic acid. by using the most avid antibody (mb 62), a latex particle agglutination test was developed which could detec ...19873116036
beta-lactamase production in the upper respiratory tract flora in relation to antibiotic consumption: a study in children attending day nurseries.the occurrence of beta-lactamase production in haemophilus influenzae, branhamella catarrhalis and moraxella nonliquefaciens was compared in 191 healthy children attending day nurseries in 2 municipalities differing with regard to the prescription rate of beta-lactam antibiotics. a significantly higher frequency of beta-lactamase production was recorded in m. nonliquefaciens isolated in the municipality with the higher prescription rate. a corresponding difference was not recorded for h. influen ...19883261446
differentiation of some species of neisseriaceae and other bacterial groups by dna-dna hybridization.dna-dna hybridization using total genomic dna probes may represent a way of differentiating between miscellaneous bacterial species. this was studied with type and reference strains of 20 species in moraxella, kingella, and other selected gram-negative groups. both radioactive and biotin labelling were employed. most of the species examined were easily distinguished, such as moraxella (branhamella) catarrhalis, m.(b.) ovis, m. atlantae, m. phenylpyruvica, m. osloensis, neisseria elongata, n. men ...19892730785
botryomycosis caused by moraxella nonliquefaciens.botryomycosis is a bacterial infection characterized by the presence of grains of organisms in the tissue. although staphylococcus aureus is the most common causative agent, other bacteria have been reported to cause botryomycosis. several factors have been hypothesized to be important in the pathogenesis of botryomycosis, including foreign bodies, quantity and virulence of organisms, and host immunity. the resulting infection tends to be resistant to antibiotic therapy. we report a case of botr ...19892702856
infections caused by moraxella, moraxella urethralis, moraxella-like groups m-5 and m-6, and kingella kingae in the united states, 1953-1980.from 1953 to 1980 the centers for disease control received 933 isolates of bacteria belonging to species of the genus moraxella, moraxella-like moraxella urethralis, now renamed oligella urethralis, unnamed groups m-5 and m-6, and kingella kingae. ordinarily sterile sites were the source of 233 isolates. moraxella nonliquefaciens, the most common isolate (356 strains), was recovered from upper respiratory or ocular sites in 208 (58%) of the cases. moraxella osloensis was next most common (199 st ...19902359906
identity between polysaccharide antigens of moraxella nonliquefaciens, group b neisseria meningitidis, and escherichia coli k1 (non-o acetylated).a surface polysaccharide antigen of moraxella nonliquefaciens, reported to be cross-reactive with the capsular polysaccharides of group b neisseria meningitidis and escherichia coli k1 (k. błvre, k. bryn, o. closs, n. hagen, and l. o. froholm, niph ann. 6:65-73, 1983), was isolated, purified, and characterized chemically, immunologically, and by nuclear magnetic resonance. this polysaccharide was shown to be a linear homopolymer of alpha (2----8)-linked n-acetylneuraminic acid, identical to the ...19911898915
the type 4 pilin of moraxella nonliquefaciens exhibits unique similarities with the pilins of neisseria gonorrhoeae and dichelobacter (bacteroides) nodosus.moraxella nonliquefaciens is a bacterium which is part of the normal flora of the human upper respiratory tract and is an occasional cause of disease. using a previously cloned type 4 pilin gene (tfpq) from moraxella bovis as a hybridization probe, we have cloned an 826 bp sau3 ai fragment which contains an m. nonliquefaciens type 4 pilin gene (tfpa) from strain nctc 7784. the pilin gene is expressed in escherichia coli. we have examined nctc 7784 and nine other m. nonliquefaciens strains by gen ...19911770363
production of betalactamase by respiratory tract bacteria in children: relationship to antibiotic use.sales of antibiotics have increased in sweden during the past decade. this has been paralleled by an increase in the frequency of beta-lactamase-producing respiratory tract bacteria. to investigate the effects of regional differences in use of antibiotics on beta-lactamase production in respiratory tract bacteria, we collected nasopharyngeal specimens and information about antibiotic use from 1133 children attending day-care centres in four rural municipalities with low use, and one urban munici ...19921589657
differentiation of moraxella nonliquefaciens, m. lacunata, and m. bovis by using multilocus enzyme electrophoresis and hybridization with pilin-specific dna probes.genetic relationships among strains of moraxella nonliquefaciens, m. lacunata, and m. bovis were studied by using multilocus enzyme electrophoresis and dna-dna hybridization. the 74 isolates analyzed for electrophoretic variation at 12 enzyme loci were assigned to 59 multilocus genotypes. the multilocus genotypes were grouped in four major clusters, one representing strains of m. nonliquefaciens, two representing strains of m. lacunata, and one comprising strains of m. bovis and the single strai ...19921452691
characterization of cell-bound papain-soluble beta-lactamases in bro-1 and bro-2 producing strains of moraxella (branhamella) catarrhalis and moraxella nonliquefaciens.in moraxella (branhamella) catarrhalis and moraxella nonliquefaciens strains isolated from clinical specimens in the south of sweden two variants of beta-lactamase were distinguished by isoelectric focusing (ief). the bro-1 (ravasio type) enzyme was the most common in branhamella catarrhalis, constituting about 90% of the beta-lactamase found in this species, while the bro-2 enzyme (1908 type) was as common as bro-1 in moraxella nonliquefaciens. the determinants mediating the production of bro-1 ...19921396750
endophthalmitis caused by beta-lactamase-positive moraxella nonliquefaciens. 19938488926
endophthalmitis caused by unusual gram-negative bacilli: three case reports and review.endophthalmitis due to gram-negative bacilli has been associated with a high degree of vision loss. we report three cases due to the nonenteric gram-negative bacilli moraxella nonliquefaciens, haemophilus paraphrophilus, and multidrug-resistant haemophilus influenzae. the features of these cases are compared with those of other reported cases of endophthalmitis due to unusual nonenteric gram-negative bacilli. fifty-eight percent of patients had no vision in the affected eye after treatment. earl ...19938268350
panaritium ossale et articulare caused by moraxella nonliquefaciens.only little is known about soft tissue, bone or joint infections caused by moraxella spp. a case of panaritium ossale et articulare caused by moraxella nonliquefaciens in an 80-year-old patient immunocompromised by diabetes mellitus and liver insufficiency is reported here. surgery, local antiseptic treatment, and therapy with aminopenicillins plus beta-lactamase-inhibitors led to complete cure of the infection within 10 days.19938300255
sequence divergence in two tandemly located pilin genes of eikenella corrodens.eikenella corrodens normally inhabits the human respiratory and gastrointestinal tracts but is frequently the cause of abscesses at various sites. using the n-terminal portion of the moraxella nonliquefaciens pilin gene as a hybridization probe, we cloned two tandemly located pilin genes of e. corrodens 31745, ecpc and ecpd, and expressed the two pilin genes separately in escherichia coli. a comparison of the predicted amino acid sequences of e. corrodens 31745 ecpc and ecpd revealed considerabl ...19938478080
identification of moraxella-like bacteria isolated from caprine and ovine nasal flora.twenty four moraxella related bacterias were isolated from healthy caprine and ovine nasal swabs and were investigated by classic biochemical tests and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins in comparison with 9 reference strains. proteolytic and haemolytic strains were investigated by electron microscopy. the biochemical results clustered field isolates in four groups corresponding to branhamella and moraxella species. proteolytic, haemoly ...19938322548
relatedness of three species of "false neisseriae," neisseria caviae, neisseria cuniculi, and neisseria ovis, by dna-dna hybridizations and fatty acid analysis.dna-dna hybridization was used to determine the levels of genomic relatedness of the three species of "false neisseriae," neisseria caviae, neisseria cuniculi, and neisseria ovis. the reference strains of these species exhibited high levels of intraspecies relatedness (93 to 100% for n. caviae, 79 to 100% for n. cuniculi, and 68 to 100% for n. ovis) but low levels of interspecific relatedness (less than 34%) to each other and to various species belonging to the beta subclass of the proteobacteri ...19938494736
a polysaccharide produced by a mucoid strain of moraxella nonliquefaciens with a 2-acetamido-2-deoxy-5-o-(3-deoxy-beta-d-manno-octulopyranosyl)-beta-d- galactopyranosyl repeating unit.a capsular polysaccharide, isolated from the mucoid moraxella nonliquefaciens strain 3828/60, has been investigated by component analyses, periodate oxidation, methylation analyses, mass spectrometry, 1h and 13c nmr spectroscopy, and hydrolysis to give a disaccharide that was isolated and characterised. the results showed that the polysaccharide has the repeating unit-->3)-beta-d- galpnac-(1-->5)-beta-kdo p-(2-->, with approximately 40% of o-8 of kdo being acetylated.19938358744
a membrane-bound precursor beta-lactamase in strains of moraxella catarrhalis and moraxella nonliquefaciens that produce periplasmic bro-1 and bro-2 beta-lactamases.by employing the non-ionic detergent triton x-100, a membrane-bound beta-lactamase was extracted from strains of moraxella (branhamella) catarrhalis and moraxella nonliquefaciens that produce bro-1 and bro-2 beta-lactamases. unlike bro-1 and bro-2, which exhibit multiple major bands on isoelectric focusing (ief), the membrane-bound enzyme focused as a single ief band at a pi of 6.20, which was not present with either of the other two enzymes. the membrane-bound beta-lactamase could be extracted ...19938463169
acute thyroiditis caused by moraxella nonliquefaciens.a 43-year-old woman presented with thyroiditis caused by moraxella nonliquefaciens. antibiotic therapy resulted in complete recovery. the literature on m. nonliquefaciens and acute bacterial thyroiditis is reviewed. this is the first documented case of a thyroiditis caused by m. nonliquefaciens.19947808579
identification of moraxella bovis and related species from calves with ibk and goats by qualitative genetic transformation assay.eight moraxella bovis strains isolated from bovine pink eye, 16 moraxella bovis related strains isolated from healthy goats nasal flora, one nonhemolytic moraxella sp. isolated from bovine conjunctivitis and different collection strains of moraxella and branhamella genus were studied through the combined use of qualitative genetic transformation assay and the ability to grow on a moraxella bovis defined medium (medium mb). crude dna extracted from the strains studied were used to transform two m ...19947839756
[moraxella nonliquefaciens spondylodiscitis]. 19947833897
complement deficiency predisposes for meningitis due to nongroupable meningococci and neisseria-related bacteria.nongroupable meningococci or bacteria related to the genus neisseria rarely cause meningitis. complement deficiency has been identified as a major predisposing factor for meningococcal disease. to assess whether patients with meningitis due to such strains have a complement deficiency, we studied 12 persons. six patients had meningitis due to nongroupable strains of meningococci, and six patients had meningitis due to moraxella species or acinetobacter species. inherited complement component c7 ...19948075270
moraxella nonliquefaciens septic arthritis in a patient undergoing hemodialysis. 19958645799
differentiation of mycobacterium ulcerans, m. marinum, and m. haemophilum: mapping of their relationships to m. tuberculosis by fatty acid profile analysis, dna-dna hybridization, and 16s rrna gene sequence analysis.although mycobacterium ulcerans, m. marinum, and m. haemophilum are closely related, their exact taxonomic placements have not been determined. we performed gas chromatography of fatty acids and alcohols, as well as dna-dna hybridization and 16s rrna gene sequence analysis, to clarify their relationships to each other and to m. tuberculosis. m. ulcerans and m. marinum were most closely related to one another, and each displayed very strong genetic affinities to m. tuberculosis; they are actually ...19989542909
isolation and characterization of three streptococcus pneumoniae transformation-specific loci by use of a lacz reporter insertion vector.although more than a dozen new proteins are produced when streptococcus pneumoniae cells become competent for genetic transformation, only a few of the corresponding genes have been identified to date. to find genes responsible for the production of competence-specific proteins, a random lacz transcriptional fusion library was constructed in s. pneumoniae by using the insertional lacz reporter vector pevp3. screening the library for clones with competence-specific beta-galactosidase (beta-gal) p ...19989573156
isolation of porphyromonas gingivalis and detection of immunoglobulin a specific to fimbrial antigen in gingival crevicular fluid.the present study evaluated the prevalence of porphyromonas gingivalis and the correlation between the bacterial culture method and the detection of immunoglobulin a (iga) specific to the p. gingivalis fimbrial antigen in gingival crevicular fluid (gcf). p. gingivalis was isolated from 78.3% of subgingival plaque samples obtained from active sites and 34.7% of those from inactive sites of periodontal patients. p. gingivalis was isolated from only 4.7% of healthy subjects (control group). immunog ...19989666013
moraxella (branhamella) catarrhalis bro beta-lactamase: a lipoprotein of gram-positive origin?in the past 20 years, bro beta-lactamase-producing moraxella catarrhalis strains have emerged. we show that bro is expressed as a 33-kda lipoprotein associated with the inner leaflet of the outer membrane. to our knowledge, this is the first description of a lipidated beta-lactamase in a gram-negative species.199910438784
molecular characterization of the beta-lactamases from clinical isolates of moraxella (branhamella) catarrhalis obtained from 24 u.s. medical centers during 1994-1995 and 1997-1998.the beta-lactamases from 403 moraxella (branhamella) catarrhalis clinical isolates obtained during 1994-1995 and 1997-1998 u.s. multicenter surveillance studies were characterized by isoelectric focusing. the overall prevalences of the bro-1 and bro-2 enzymes among beta-lactamase-positive isolates were estimated to be 97.5 and 2.5%, respectively. the minimum inhibitory concentrations (mics) of ampicillin for all bro-2-producing isolates were </=1 microg/ml; however, numerous beta-lactamase-posit ...200010639381
type iv pilus genes pila and pilc of pseudomonas stutzeri are required for natural genetic transformation, and pila can be replaced by corresponding genes from nontransformable species.pseudomonas stutzeri lives in terrestrial and aquatic habitats and is capable of natural genetic transformation. after transposon mutagenesis, transformation-deficient mutants were isolated from a p. stutzeri jm300 strain. in one of them a gene which coded for a protein with 75% amino acid sequence identity to pilc of pseudomonas aeruginosa, an accessory protein for type iv pilus biogenesis, was inactivated. the presence of type iv pili was demonstrated by susceptibility to the type iv pilus-dep ...200010735861
isolation of moraxella canis from an ulcerated metastatic lymph node.moraxella canis was isolated in large numbers from an ulcerated supraclavicular lymph node of a terminal patient, who died a few days later. although the patient presented with septic symptoms and with a heavy growth of gram-negative diplococci in the lymph node, blood cultures remained negative. m. canis is an upper-airway commensal from dogs and cats and is considered nonpathogenic for humans, although this is the third reported human isolate of this species.200011015424
activity and cross-reactivity of antibodies induced in mice by immunization with a group b meningococcal conjugate.the capsular polysaccharide of group b neisseria meningitidis is composed of a linear homopolymer of alpha(2-8) n-acetyl neuraminic acid or polysialic acid (psa) that is also carried by isoforms of the mammalian neural cell adhesion molecule (ncam), which is especially expressed on brain cells during development. here we analyzed the ability of antibodies induced by the candidate vaccine n-propionyl polysaccharide tetanus toxoid conjugate to recognize psa-ncam. we hyperimmunized mice to produce ...200111598089
mutant bacteriophage with non-catalytic endosialidase binds to both bacterial and eukaryotic polysialic acid and can be used as probe for its detection.there is a molecular mimicry between the polysialic acid polysaccharide of bacterial pathogens causing sepsis and meningitis, and the carbohydrate units of the neural cell adhesion molecule ncam. we investigated whether bacteriophage mutants with catalytically disabled endosialidase, which bind but do not cleave polysialic acid, could recognise and bind to bacterial and eukaryotic polysialic acid. in nitrocellulose dot blot assay the mutant bacteriophages, but not the wild-type phages, remained ...200112441664
comparative evaluation of the bd phoenix and vitek 2 automated instruments for identification of isolates of the burkholderia cepacia complex.we evaluated two new automated identification systems, the bd phoenix (becton dickinson) and the vitek 2 (biomérieux), for identification of isolates of the burkholderia cepacia complex (bcc). the test sample included 42 isolates of the highly virulent and epidemic genomovar iii, 45 isolates of b. multivorans, and 47 isolates of other members of the bcc. rates of correct identification by the bd phoenix and vitek 2 were similar when all bcc isolates were considered (50 and 53%, respectively) but ...200211980954
posttrabeculectomy endophthalmitis caused by moraxella nonliquefaciens.moraxella nonliquefaciens, a commensal organism of the upper respiratory tract, is generally considered to have low pathogenic potential. we report here two cases of severe endophthalmitis occurring 9 years and 2 months after glaucoma filtration surgery, respectively. apart from sulfonamide, very low mics were recorded for several antibiotics tested. identification was based on phenotypic characteristics in combination with sequencing of the 16s rrna gene.200212089304
presumed endocarditis caused by bro beta-lactamase-producing moraxella lacunata in an infant with fallot's tetrad.a case of presumed endocarditis caused by moraxella lacunata in a 15-month-old male infant with fallot's tetrad is described. this infection may have occurred as the result of transmission of this organism between the father and his son. this is the first report of bro beta-lactamase-producing m. lacunata causing presumed endocarditis.200314605192
the moraxella catarrhalis immunoglobulin d-binding protein mid has conserved sequences and is regulated by a mechanism corresponding to phase variation.the prevalence of the moraxella catarrhalis immunoglobulin d (igd)-binding outer membrane protein mid and its gene was determined in 91 clinical isolates and in 7 culture collection strains. eighty-four percent of the clinical moraxella strains expressed mid-dependent igd binding. the mid gene was detected in all strains as revealed by homology of the signal peptide sequence and a conserved area in the 3' end of the gene. when mid proteins from five different strains were compared, an identity o ...200312644500
mucoid nitrate-negative moraxella nonliquefaciens from three patients with chronic lung disease.mucoid strains of moraxella nonliquefaciens were recovered from the sputa of three indigenous australians with chronic lung disease. these atypical strains failed to reduce nitrate, and one strain produced beta-lactamase. while the mucoid phenotype of m. nonliquefaciens has rarely been reported, the mucoid nitrate-negative biovar has never been previously reported.200415297558
diversity of microbial sialic acid metabolism.sialic acids are structurally unique nine-carbon keto sugars occupying the interface between the host and commensal or pathogenic microorganisms. an important function of host sialic acid is to regulate innate immunity, and microbes have evolved various strategies for subverting this process by decorating their surfaces with sialylated oligosaccharides that mimic those of the host. these subversive strategies include a de novo synthetic pathway and at least two truncated pathways that depend on ...200415007099
computational identification of strain-, species- and genus-specific proteins.the identification of unique proteins at different taxonomic levels has both scientific and practical value. strain-, species- and genus-specific proteins can provide insight into the criteria that define an organism and its relationship with close relatives. such proteins can also serve as taxon-specific diagnostic targets.200516305751
multiplex pcr assay that identifies the major lipooligosaccharide serotype expressed by moraxella catarrhalis clinical isolates.a heterologous cluster of glycosyltransferase genes was identified in the three moraxella catarrhalis los serotype strains. multiple pcr primers designed to this region amplified products that differentiate between the serotypes more rapidly and efficiently than previously described serological analyses. this assay will be valuable for clinical and research-based studies.200516333114
moraxella catarrhalis bacterium without endotoxin, a potential vaccine candidate.lipooligosaccharide (los) is a major surface component of moraxella catarrhalis and a possible virulence factor in the pathogenesis of human infections caused by this organism. the presence of los on the bacterium is an obstacle to the development of vaccines derived from whole cells or outer membrane components of the bacterium. an lpxa gene encoding udp-n-acetylglucosamine acyltransferase responsible for the first step of lipid a biosynthesis was identified by the construction and characteriza ...200516239560
identification of acinetobacter baumannii by detection of the blaoxa-51-like carbapenemase gene intrinsic to this species.bla(oxa-51-like) was sought in clinical isolates of acinetobacter species in a multiplex pcr, which also detects bla(oxa-23-like) and class 1 integrase genes. all isolates that gave a band for bla(oxa-51-like) identified as a. baumannii. this gene was detected in each of 141 isolates of a. baumannii but not in those of 22 other acinetobacter species.200616891520
separate pathways for o acetylation of polymeric and monomeric sialic acids and identification of sialyl o-acetyl esterase in escherichia coli k1.o acetylation at carbon positions 7 or 9 of the sialic acid residues in the polysialic acid capsule of escherichia coli k1 is catalyzed by a phase-variable contingency locus, neuo, carried by the k1-specific prophage, cus-3. here we describe a novel method for analyzing polymeric sialic acid o acetylation that involves the release of surface sialic acids by endo-n-acetylneuraminidase digestion, followed by fluorescent labeling and detection of quinoxalinone derivatives by chromatography. the res ...200616923886
quantitative determination of free-dna uptake in river bacteria at the single-cell level by in situ rolling-circle amplification.detection of plasmid dna uptake in river bacteria at the single-cell level was carried out by rolling-circle amplification (rca). uptake of a plasmid containing the green fluorescent protein gene (gfp) by indigenous bacteria from two rivers in osaka, japan, was monitored for 506 h using this in situ gene amplification technique with optimized cell permeabilization conditions. plasmid uptake determined by in situ rca was compared to direct counts of cells expressing gfp under fluorescence microsc ...200616957252
comparison of a taqman-based real-time polymerase chain reaction with conventional tests for the detection of trichomonas vaginalis.to compare a taqman-based real-time polymerase chain reaction (pcr) with conventional pcr, culture, and wet-mount microscopy for the diagnosis of trichomoniasis in women.200717090567
the outer membrane secretin pilq from neisseria meningitidis binds dna.neisseria meningitidis is naturally competent for transformation throughout its growth cycle. transformation in neisserial species is coupled to the expression of type iv pili, which are present on the cell surface as bundled filamentous appendages, and are assembled, extruded and retracted by the pilus biogenesis components. during the initial phase of the transformation process, binding and uptake of dna takes place with entry through a presumed outer-membrane channel into the periplasm. this ...200717464074
biochemical characterization of a neisseria meningitidis polysialyltransferase reveals novel functional motifs in bacterial sialyltransferases.the extracellular polysaccharide capsule is an essential virulence factor of neisseria meningitidis, a leading cause of severe bacterial meningitis and sepsis. serogroup b strains, the primary disease causing isolates in europe and america, are encapsulated in alpha-2,8 polysialic acid (polysia). the capsular polymer is synthesized from activated sialic acid by action of a membrane-associated polysialyltransferase (nmb-polyst). here we present a comprehensive characterization of nmb-polyst. diff ...200717662040
identification of amino acid residues at the active site of endosialidase that dissociate the polysialic acid binding and cleaving activities in escherichia coli k1 bacteriophages.endosialidase (endo-n-acetylneuraminidase) is a tailspike enzyme of bacteriophages specific for human pathogenic escherichia coli k1, which specifically recognizes and degrades polysia (polysialic acid). polysia is also a polysaccharide of the capsules of other meningitis- and sepsis-causing bacteria, and a post-translational modification of the ncam (neural cell-adhesion molecule). we have cloned and sequenced three spontaneously mutated endosialidases of the pk1a bacteriophage and one of the p ...200717394421
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods.to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
comparison of a taqman-based real-time polymerase chain reaction with conventional tests for the detection of trichomonas vaginalis.to compare a taqman-based real-time polymerase chain reaction (pcr) with conventional pcr, culture, and wet-mount microscopy for the diagnosis of trichomoniasis in women.200717090567
identification of neisserial dna binding components.neisseria meningitidis, a causative agent of meningitis and septicaemia, expresses type iv pili, a feature correlating with the uptake of exogenous dna from the environment by natural transformation. the outer membrane complex pilq, through which pili are extruded and retracted, has previously been shown to bind dna in its pore region. in order to further elucidate how dna is transported across the membranes, we searched for dna binding proteins within the meningococcal inner membrane. inner mem ...200919246756
recurrent gram-negative bloodstream infection: a 10-year population-based cohort study.recurrent gram-negative bloodstream infection (bsi) has not been evaluated in a population-based setting; therefore, we performed a population-based retrospective cohort study to examine the incidence, recurrence, and mortality rates of gram-negative bsi.201020378069
clinical significance of a single staphylococcus lugdunensis-positive blood culture. 201121270222
infective endocarditis caused by moraxella nonliquefaciens in a percutaneous aortic valve replacement.first case of infective endocarditis on a percutaneous aortic valve replacement due to moraxalla nonliquefaciens in 64 year old woman. it was successfully treated medically with antibiotics. she had not suitable for surgical aortic valve replacement due to 3 sternotomies for thymoma resection and subsequent radiotherapy with blocked major thoracic veins. due to her azathioprine immunosuppresion (myasthenia) she may have been at increased endocarditis risk. we suggest prophylactic antibiotics at ...201121640938
Capsular polysaccharide vaccine for Group B Neisseria meningitidis, Escherichia coli K1, and Pasteurella haemolytica A2.We reviewed the literature that is the basis for our proposal that (2?8)-a-Neu5Ac conjugates will be safe and effective vaccines for Group B meningococci (GBMs), Escherichia coli K1, and Pasteurella haemolytica A2. Although (2?8)-a-Neu5Ac is a virulence factor and a protective antigen of these three pathogens, it is also a component of normal tissues (neural cell adhesion molecule). Natural, anti-(2?8)-a-Neu5Ac present in most adults, vaccine-induced antibodies, and even high levels of spontaneo ...201122025709
integrated analysis of three bacterial conjunctivitis trials of besifloxacin ophthalmic suspension, 0.6%: etiology of bacterial conjunctivitis and antibacterial susceptibility profile.the purpose of this paper is to report on the bacterial species isolated from patients with bacterial conjunctivitis participating in three clinical trials of besifloxacin ophthalmic suspension, 0.6%, and their in vitro antibacterial susceptibility profiles.201122034556
comparison of bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to bd phoenix automated microbiology system for identification of gram-negative bacilli.we compared the bd phoenix automated microbiology system to the bruker biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) system for identification of gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant results. the bd phoenix correctly identified 363 (83%) and 330 (75%) isolates to the genus and species level, respectively. the bruker biotyper correctly identified 408 (93%) and 360 (82 ...201121209160
microbial profile and antibiotic susceptibility of culture-positive bacterial endophthalmitis.to assess the distribution of microorganisms isolated from patients with bacterial endophthalmitis and their antimicrobial susceptibility.201121336253
evaluation of the genbank, eztaxon, and bibi services for molecular identification of clinical blood culture isolates that were unidentifiable or misidentified by conventional methods.we compared the 16s rrna gene sequencing results analyzed with the genbank, eztaxon, and bibi databases for blood culture specimens for which identifications were incomplete, conflicting, or unidentifiable using conventional methods. analyses performed using genbank combined with eztaxon (kappa = 0.79) were more discriminative than those using other databases alone or in combination with a second database.201222403421
the microbiome of the middle meatus in healthy adults.rhinitis and rhinosinusitis are multifactorial disease processes in which bacteria may play a role either in infection or stimulation of the inflammatory process. rhinosinusitis has been historically studied with culture-based techniques, which have implicated several common pathogens in disease states. more recently, the nih human microbiome project has examined the microbiome at a number of accessible body sites, and demonstrated differences among healthy and diseased patients. recent dna-base ...201324386477
analysis of the sds-page patterns of outer membrane proteins from escherichia coli strains that have lost the ability to form k1 antigen and varied in the susceptibility to normal human serum.we used sds-polyacrylamide gel electrophoresis to investigate the outer membrane proteins (omps) band composition of 19 escherichia coli k1 strains that have spontaneously lost the ability to form k1 polysaccharide capsule (e. coli k1-) and demonstrated different degrees of susceptibility to the bactericidal action of normal human serum. presented results showed that there were differences between e. coli k1- strains in omps expressing capacity. the analysis performed on omps has not revealed a ...201323794053
analysis of the sds-page patterns of outer membrane proteins from escherichia coli strains that have lost the ability to form k1 antigen and varied in the susceptibility to normal human serum.we used sds-polyacrylamide gel electrophoresis to investigate the outer membrane proteins (omps) band composition of 19 escherichia coli k1 strains that have spontaneously lost the ability to form k1 polysaccharide capsule (e. coli k1-) and demonstrated different degrees of susceptibility to the bactericidal action of normal human serum. presented results showed that there were differences between e. coli k1- strains in omps expressing capacity. the analysis performed on omps has not revealed a ...201323794053
accurate identification of fastidious gram-negative rods: integration of both conventional phenotypic methods and 16s rrna gene analysis.accurate identification of fastidious gram-negative rods (gnr) by conventional phenotypic characteristics is a challenge for diagnostic microbiology. the aim of this study was to evaluate the use of molecular methods, e.g., 16s rrna gene sequence analysis for identification of fastidious gnr in the clinical microbiology laboratory.201323855986
characterization of the kingella kingae polysaccharide capsule and exopolysaccharide.recent evidence indicates that kingella kingae produces a polysaccharide capsule. in an effort to determine the composition and structure of this polysaccharide capsule, in the current study we purified capsular material from the surface of k. kingae strain 269-492 variant kk01 using acidic conditions to release the capsule and a series of steps to remove dna, rna, and protein. analysis of the resulting material by gas chromatography and mass spectrometry revealed n-acetyl galactosamine (galnac) ...201324098695
assessing genetic structure, diversity of bacterial aerosol from aeration system in an oxidation ditch wastewater treatment plant by culture methods and bio-molecular tools.airborne bacteria emissions from oxidation ditch with rotating aeration brushes were investigated in a municipal wastewater treatment plant in beijing, china. microbial samples were collected at different distances from the rotating brushes, different heights above the water surface, and different operation state over a 3-month period (april, may, and june) in order to estimate the seasonal variation and site-related distribution characteristics of the microorganisms present. the concentration o ...201322402990
rapid 16s rrna next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections.classifying individual bacterial species comprising complex, polymicrobial patient specimens remains a challenge for culture-based and molecular microbiology techniques in common clinical use. we therefore adapted practices from metagenomics research to rapidly catalog the bacterial composition of clinical specimens directly from patients, without need for prior culture. we have combined a semiconductor deep sequencing protocol that produces reads spanning 16s ribosomal rna gene variable regions ...201323734239
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