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community-acquired necrotizing fasciitis caused by acinetobacter calcoaceticus: a case report and literature review.a 61-year-old man presented with pain in the abdomen and right lower limb. he had a history of hepatitis b virus-induced liver cirrhosis, but had not been visiting the outpatient clinic and did not receive any medication. cutaneous necrosis and bulla were observed on his abdomen and right lower limb. the necrotic skin was incised, and he was diagnosed with necrotizing fasciitis. a nonfermentative gram-negative bacillus infection was confirmed from aspirated fluid and blood cultures. therefore, m ...201424507651
antimicrobial susceptibility pattern in nosocomial infections caused by acinetobacter species in asir region, saudi arabia.this study aimed at evaluating the sensitivity of antibiotics towards nosocomial infections caused by acinetobacter species. the study took place during the period dec. 2011- dec. 2012 at assir central hospital in collaboration with the department of microbiology, college of medicine, king khalid university, abha. a prospective study involving 150 patients presented with nosocomial infections due to acinetobacter species detected by bacteriological tests; direct microscopy, culture in blood agar ...201324498790
[effect of growth factors and some microelements on biosurfactant synthesis of acinetobacter calcoaceticus imv b-7241].the effect of yeast autolysate and microelements on synthesis of surface-active substances (sas, biosurfactants) was investigated under cultivation of acinetobacter calcoaceticus imv b-7241 on various carbon substrates (n-hexadecane, ethanol, glycerol). the authors have shown a possibility to substitute the yeast autolysate and microelement mixture in the composition of ethanol- and n-hexadecane-containing media by copper sulfate (0.16 micromol/l) and iron sulfate (3.6 micromol/l), and in the me ...201424479309
wired pyrroloquinoline quinone soluble glucose dehydrogenase enzyme electrodes operating at unprecedented low redox potential.we report unprecedented high current densities for the enzymatic oxidation of glucose already at 0 v versus ag/agcl. the modified electrodes were made by assembling pyrroloquinoline quinone (pqq)-soluble glucose dehydrogenase (pqq-sgdh) from acinetobacter calcoaceticus with osmium-based redox polymers and a cross-linker. both redox mediators are made of a poly(4-vinylpyridine) (pvp) polymer with os complexes tethered to the polymer backbone via long c chains, giving the os complexes flexibility ...201424475934
in vitro activity of tigecycline against patient isolates collected during phase 3 clinical trials for hospital acquired pneumonia.the in vitro activity of tigecycline was evaluated against 819 baseline pathogens isolated from 383 patients enrolled in the phase 3 clinical trial investigating the efficacy of tigecycline in hospital acquired pneumonia (hap). the trials were global, enrolling patients in 27 countries. tigecycline was active against the most prevalent pathogens in hap, including gram-positive and gram-negative strains (90% of mics ≤2 µg/ml for the entire collection). the spectrum of activity of tigecycline incl ...201024470884
plant growth-promoting bacterium acinetobacter calcoaceticus p23 increases the chlorophyll content of the monocot lemna minor (duckweed) and the dicot lactuca sativa (lettuce).acinetobacter calcoaceticus p23 is a plant growth-promoting bacterium that was isolated from the surface of duckweed (lemna aoukikusa). the bacterium was observed to colonize on the plant surfaces and increase the chlorophyll content of not only the monocotyledon lemna minor but also the dicotyledon lactuca sativa in a hydroponic culture. this effect on the lactuca sativa was significant in nutrient-poor (×1/100 dilution of h2 medium) and not nutrient-rich (×1 or ×1/10 dilutions of h2 medium) co ...201424468072
[effect of surface-active substances of acinetobacter calcoaceticus imv b-7241, rhodococcus erythropolis imv ac-5017, and nocardia vaccinii k-8 on phytopathogenic bacteria].the effect of surface-active substances (sas's) of acinetobacter calcoaceticus imv b-7241, rhodococcus erythropolis imv ac-5017, and nocardia vaccinii k-8 on phytopathogenic bacteria has been studied. it was shown that the survival of cells (10(5)-10(7) in a milliliter) of the pseudomonas and xanthomonas phytopathogenic bacteria was found to be 0-33% after treatment with sas preparations of the imv ac-5017 and imv b-7241 strains for 2 h (0.15-0.4 mg/ml). in the presence of n. vaccinii k-8 sas pr ...201424455862
oral flora of python regius kept as pets.this study was aimed at evaluating the oral bacterial flora of 60 python regius kept as pets by culture and biochemical methods. all isolates were also submitted to antimicrobial susceptibility testing using the disc diffusion method. the oral cavity of snakes sampled harboured a wide range of gram-negative bacteria mainly constituted by pseudomonas spp., morganella morganii, acinetobacter calcoaceticus, aeromonas hydrophila, but also by salmonella spp. staphylococcus spp. was the commonest gram ...201424383854
emergence of ndm-producing non-baumannii acinetobacter spp. isolated from china.one hundred and thirty-six bla oxa-51-negative strains were identified from 1,067 acinetobacter calcoaceticus-a. baumannii complex (acb complex) isolates, which were collected during october 2010 to march 2013 from 15 general hospitals in 10 cities throughout zhejiang province, china. seven of the 136 bla oxa-51-negative acb complex isolates were new delhi metallo-β-lactamase-1 (ndm-1)-positive, among which three were identified as a. nosocomialis and four were identified as a. pittii strains us ...201424306098
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
multiplex pcr to detect the genes encoding naturally occurring oxacillinases in acinetobacter spp.bacteria of the genus acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. species identification is difficult and there is a need for simple molecular methods to differentiate between the species. naturally occurring oxacillinase genes (blaoxa) have been identified in several acinetobacter species and their detection by pcr can aid in species identification. the aim of this study was to develop a multiplex pcr to identify intrinsic blao ...201424288029
identification of 50 class d β-lactamases and 65 acinetobacter-derived cephalosporinases in acinetobacter spp.whole-genome sequencing of a collection of 103 acinetobacter strains belonging to 22 validly named species and another 16 putative species allowed detection of genes for 50 new class d β-lactamases and 65 new acinetobacter-derived cephalosporinases (adc). all oxacillinases (oxa) contained the three typical motifs of class d β-lactamases, stfk, (f/y)gn, and k(s/t)g. the phylogenetic tree drawn from the oxa sequences led to an increase in the number of oxa groups from 7 to 18. the topologies of th ...201424277043
2,2'-oxo-1,1'-azobenzene: microbial transformation of rye (secale cereale l.) allelochemical in field soils byacinetobacter calcoaceticus: iii.acinetobacter calcoaceticus, a gram-negative bacterium isolated from field soil, was found to be responsible for the biotransformation of 2(3h)-benzoxazolinone (boa) to 2,2'-oxo-1,1'-azobenzene (azob). experiments were conducted to evaluate the transformation of boa to azob by this microbe in sterile and nonsterile soil. transformation studies with soils inoculated witha. calcoaceticus indicated that the production of azob increased linearly with the concentration of boa in sterile soil and show ...199124257881
use of growing cells of pseudomonas aeruginosa for synthesis of the natural vanillin via conversion of isoeugenol.the great demand of people for consumption of natural additives resulted in producing natural vanillin. there are plant sources and chemical procedures for vanillin production but microbial bioconversions are being sought as a suitable alternative. in the present work, the ability to produce vanillin from isoeugenol was screened using growing cultures of various bacteria. among the 56 strains of bacteria isolated from the soil environments of iran, a gram-negative rod designated as strain ispc2 ...201124250410
synthesis, optimization, and characterization of silver nanoparticles from acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics.the development of nontoxic methods of synthesizing nanoparticles is a major step in nanotechnology to allow their application in nanomedicine. the present study aims to biosynthesize silver nanoparticles (agnps) using a cell-free extract of acinetobacter spp. and evaluate their antibacterial activity.201324235826
bacterial desorption from food container and food processing surfaces.the desorption ofstaphylococcus aureus, acinetobacter calcoaceticus, and a coryneform from the surfaces of materials used for manufacturing food containers (glass, tin plate, and polypropylene) or postprocess canning factory conveyor belts (stainless steel and nylon) was investigated. the effect of time, ph, temperature, and adsorbed organic layers on desorption was studied.s. aureus did not detach from the substrata at any ph investigated (between ph 5 and 9).a. calcoaceticus and the coryneform ...198824203003
types and prevalence of carbapenem-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex in northern taiwan.the frequency of the carbapenem-resistant acinetobacter calcoaceticus-acinetobacter baumannii (cracb) complex increases annually in our hospitals. however, the types and prevalence of carbapenemases among isolates still remain unclear. in this study, we identified and collected 672 carbapenem-resistant isolates from a medical center in northern taiwan between april and december of 2010. there were 577 genospecies 2 (acinetobacter baumannii), 79 genospecies 13tu, and 16 genospecies 3 isolates. th ...201424145535
improvement of maldi-tof ms profiling for the differentiation of species within the acinetobacter calcoaceticus-acinetobacter baumannii complex.maldi-tof ms is currently becoming the method of choice for rapid identification of bacterial species in routine diagnostics. yet, this method suffers from the inability to differentiate reliably between some closely related bacterial species including those of the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex, namely a. baumannii and acinetobacter nosocomialis. in the present study, we evaluated a protocol which was different from that used in the bruker daltonics identifica ...201324054697
screening and characterization of a novel alkaline lipase from acinetobacter calcoaceticus 1-7 isolated from bohai bay in china for detergent formulation.a novel alkaline lipase-producing strain 1-7 identified as acinetobacter calcoaceticus was isolated from soil samples collected from bohai bay, china, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. the lipase from strain 1-7 showed the maximum activity at ph 9.0 under 40 °c. one interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. it is also stable at a broad range of phs from 4.0 to ...201224031813
enterobacteriaceae in mouth and cloaca of podocnemis expansa and p. unifilis (testudines: chelonia) populations of national park of araguaia plains, brazil.shigella flexnerii and escherichia coli were the most frequent gram-negative bacteria found in the mouth cavity and cloacae of the turtles podocnemis expansa and p. unifilis on beaches in the national park of araguaia, brazil. reptiles are known as salmonella carriers, despite rarely isolated in these turtles.201124031664
concomitant production of protease and lipase by bacillus licheniformis vsg1: production, purification and characterization.this study was aimed at producing protease and lipase simultaneously on a common medium by bacillus licheniformis vsg1, which was isolated from a tannery effluent. the effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. both those enzymes were produced under optimized conditions like ph, temperature and incubation time. the enzyme mixture comprising of both protease and lipase was purified by ammonium sulp ...201024031479
active surveillance for asymptomatic colonization with multidrug-resistant gram negative bacilli among injured service members--a three year evaluation.in response to the high rates of colonization and infection by multidrug-resistant gram-negative bacilli (mdr gnb), many military treatment facilities (mtfs) have implemented additional infection control practices, such as active surveillance cultures for asymptomatic colonization. results of surveillance cultures (june 2009 - may 2012) collected from patients at landstuhl regional medical center (landstuhl rmc), germany, and three u.s. mtfs were analyzed to evaluate trends in mdr gnb colonizati ...201324011372
isolation and characterization of a novel ε-caprolactam-degrading microbe, acinetobacter calcoaceticus, from industrial wastewater by chemostat-enrichment.for the isolation of a ε-caprolactam-degrading microbe from wastewaters of a factory producing caprolactam, we applied a chemostat-enrichment technique with a selective medium containing caprolactam as sole source of carbon and nitrogen. this allowed for the isolation of a novel caprolactam-degrading microbe, identified as acinetobacter calcoaceticus. the strain had a critical tolerance of 19 g caprolactam l(-1) in minimal medium, which is higher than any previously reported caprolactam-degradin ...201323974491
a self-sufficient baeyer-villiger biocatalysis system for the synthesis of ɛ-caprolactone from cyclohexanol.in order to establish a new route for ɛ-caprolactone production from the corresponding cyclohexanol with an internal cofactor recycling for nadph, a recently redesigned thermostable polyol dehydrogenase (pdh) and the cyclohexanone monooxygenase (chmo) from acinetobacter calcoaceticus were combined. first, the expression of pdh could be improved 4.9-fold using e. coli c41 with co-expression of chaperones. both enzymes were also successfully co-immobilized on glutaraldehyde-activated support (reli ...201323931695
[influence of ph on synthesis of acinetobacter calcoaceticus imv b-7241 biosurfactants].synthesis of extracellular metabolites with surface-active and emulsifying properties, ph being maintained at the level of 5.8-8.0, in the process of cultivation of acinetobacter calcoaceticus imv b-7241 in the medium with ethanol (2%, volume part) was investigated. it is established that the neutral value of ph is optimal for synthesis of surface-active substances (sas, biosurfactants) of a. calcoaceticus imv b-7241. the maintenance of ph at the level of 7.0 with the help of koh solution was ac ...201323866584
identification of opportunistic pathogenic bacteria in drinking water samples of different rural health centers and their clinical impacts on humans.international drinking water quality monitoring programs have been established in order to prevent or to reduce the risk of contracting water-related infections. a survey was performed on groundwater-derived drinking water from 13 different hospitals in the mahabubnagar district. a total of 55 bacterial strains were isolated which belonged to both gram-positive and gram-negative bacteria. all the taxa were identified based on the 16s rrna gene sequence analysis based on which they are phylogenet ...201323862144
real-time fluorescence loop mediated isothermal amplification for the detection of acinetobacter baumannii.detection of acinetobacter baumannii has been relying primarily on bacterial culture that often fails to return useful results in time. although dna-based assays are more sensitive than bacterial culture in detecting the pathogen, the molecular results are often inconsistent and challenged by doubts on false positives, such as those due to system- and environment-derived contaminations. in addition, these molecular tools require expensive laboratory instruments. therefore, establishing molecular ...201323843955
biodegradation of triton x-100 and its primary metabolites by a bacterial community isolated from activated sludge.a set of studies was carried using a continuous flow biodegradation unit in order to isolate a microbial community capable of efficient and complete utilization of octylphenol ethoxylates from activated sludge. increasing concentrations of triton x-100 (in the range of 1-1000 mg/l) were applied over a time period of 35 days in order to select microorganisms, which exhibit high tolerance towards this surfactant. the fate of the surfactant and its primary degradation products was assessed by hplc/ ...201323770380
isolation and structural characterization of a (kdo-isosteric) d-glycero-α-d-talo-oct-2-ulopyranosidonic acid (ko) interlinking lipid a and core oligosaccharide in the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305.in acinetobacter calcoaceticus nctc 10305 and a. haemolyticus nctc 10305 lipopolysaccharide (lps) a kdo (3-deoxy-d-manno-oct-2-ulosonic acid)-related octulosonic acid (ko) interlinks the lipid a with the core-oligosaccharide. this ko replaces the first kdo (kdo(i)) attached to the lipid a backbone in the lps. the only structural difference between kdo and ko is the 3-hydroxylation. after the discovery of the final step in ko-biosynthesis it is now generally accepted that ko is structurally relat ...201323743267
development of a whole-cell biocatalyst with nadph regeneration system for biosulfoxidation.a formate dehydrogenase gene (fdh) originated from candida boidinii was co-expressed in e. coli bl21 (de3) with the cyclohexanone monooxygenase gene (chmo) cloned from acinetobacter calcoaceticus ncimb 9871. the co-expression system was then used as a whole-cell biocatalyst to synthesize chiral phenyl methyl sulfoxide (pmso) from thioanisole (pms) and the reaction conditions were investigated. when the initial concentration of pms was 20 mm, the specific productivity of pmso in this system was 2 ...201323729190
molecular epidemiology, antimicrobial susceptibility and carbapenemase resistance determinants among acinetobacter baumannii clinical isolates in taiwan.emerging carbapenem resistance among acinetobacter baumannii clinical isolates is a worldwide problem. infections caused by a. baumannii are increasing and demonstrate high mortality rates. this study aimed to establish a nationwide surveillance of antimicrobial susceptibility, carbapenemase genes, and clonal relationships of a. baumannii clinical isolates in taiwan.201423726465
[effect of univalent cations on synthesis of surfactants by acinetobacter calcoaceticus imv b-7241].the effect of univalent cations on activity of key enzymes of c2-metabolism has been investigated in the producer of biosurfactants, acinetibacter calcoaceticus imv b-7241 grown on ethanol. it was established that potassium cations are inhibitors of pyroquinolinequinone-dependent alcohol- and acetaldehyde dehydrogenases, the enzymes of biosynthesis of surface-active aminolipids (nadp-dependent glutamate dehydrogenase) and glycolipids (phosphoenopyruvate (phep)-carboxikinase), while ammonium cati ...201323720959
identification and functional characteristics of chlorpyrifos-degrading and plant growth promoting bacterium acinetobacter calcoaceticus.a bacterial strain d10 with strong ability of degrading chlorpyrifos was isolated from rhizosphere of chives contaminated with pesticide. it was found that it's capable of utilizing chlorpyrifos as the sole source of carbon for growth, and within the first 4 days the extent of degradation at initial concentration of 100 mg l(-1) was 60.0%. it also showed a high ability of degrading chlorpyrifos in sterilized soil, and the degradation reached up to 60.2% after 18 days. in addition, the strain d10 ...201423712768
increased lysine content is the main characteristic of the soluble form of the polyamide cyanophycin synthesized by recombinant escherichia coli.cyanophycin, a polyamide of cyanobacterial or noncyanobacterial origin consisting of aspartate, arginine, and lysine, was synthesized in different recombinant strains of escherichia coli expressing cpha from synechocystis sp. strain pcc 6308 or pcc 6803, anabaena sp. strain pcc 7120, or acinetobacter calcoaceticus adp1. the molar aspartate/arginine/lysine ratio of the water-soluble form isolated from a recombinant strain expressing cpha6308 was 1:0.5:0.5, with a lysine content higher than any ev ...201323686266
acinetobacter pittii and acinetobacter nosocomialis among clinical isolates of the acinetobacter calcoaceticus-baumannii complex in sichuan, china.among 82 clinical isolates of the acinetobacter calcoaceticus-baumannii complex recovered in 13 hospitals of sichuan, china, in 2011, 13 were acinetobacter pittii and 2 were acinetobacter nosocomialis. multilocus sequence typing revealed a novel sequence type (st) of a. nosocomialis and 7 novel sts of a. pittii. most isolates were hospital-acquired and colonized in the respiratory tract, while 6 cases with pneumonia due to a. pittii were identified. this study provided a snapshot of the local in ...201323639796
complete genome sequence of an acinetobacter strain harboring the ndm-1 gene.the ndm-1 gene is a significant public health concern. acinetobacter is one of the most prevalent opportunistic pathogens causing recent nosocomial infections with ndm-1, and drug-resistant strains pose serious threats to public health worldwide. herein, we present the genomic sequence of acinetobacter calcoaceticus subsp. anitratus xm1570, a multidrug-resistant isolate that carries the blandm-1 gene.201323599286
defining resistance in acinetobacter calcoaceticus-acinetobacter baumannii complex strains. 201323580585
label-free and selective bacteria detection using a film with transferred bacterial configuration.specific identification of bacteria has been achieved through precisely transferred bacterial structure on the surface of overoxidized polypyrrole (oppy) film. the recognition of target bacteria was successfully carried out in real time using oppy film in combination with dielectrophoresis. the unique combination of both techniques made the specific detection of template bacilli possible at concentrations as low as 10(3) cfu/ml within 3 min, without any bacterial pretreatment. the observation of ...201323577783
inoculation of phosphate solubilizing bacteria for the improvement of lead accumulation by brassica juncea.two phosphate-solubilizing bacterial strains were isolated and identified as acinetobacter calcoaceticus yc-5a and enterobacter agglomerans kmc-7 based on the 16s rrna gene sequence analysis. a. calcoaceticus yc-5a is less well known as a phosphate-solubilizing plant-associated bacterium. the plant growth-promoting properties of the phosphate-solubilizing bacteria (psb) were characterized in vitro, including their phosphate-solubilizing activities and their capabilities for producing indole-3-ac ...201323530360
[effect of cu2+ on synthesis of biosurfactants of acinetobacter calcoaceticus imv b-7241 and rhodococcus erythropolis imv ac-5017].synthesis of biosurfactants (surface-active substances, sas) was investigated under the conditions of growth of rhodococcus erythropolis imv ac-5017 and acinetobacter calcoaceticus imv b-7241 on hydrophobic (n-hexadecane, liquid paraffins, sunflower oil) and hydrophilic (ethanol) substrates depending on concentration (0.01-0.5 mm) and time of copper cations introduction in the medium. it is established that cu2+ addition in the exponential phase of growth of the strains imv b-7241 and imv ac-501 ...201623516834
emerging threat of multidrug resistant bugs--acinetobacter calcoaceticus baumannii complex and methicillin resistant staphylococcus aureus.infections caused by bacteria such as multidrug resistant (mdr) acinetobacter spp. and methicillin-resistant staphylococcus aureus (mrsa) constitute a worldwide pandemic. without gathering information about these strains, we cannot reduce the morbidity and mortality due to infections caused by these notorious bugs.201323497675
hexavalent chromium reduction and accumulation by acinetobacter ab1 isolated from fez tanneries in morocco.hexavalent chromium reduction and accumulation by acinetobacter ab1 isolated from fez tanneries effluents were tested. the effects of some environmental factors such as ph, temperature, and exposure time on cr(vi) reduction and resistance were investigated. we found that this strain was able to resist to concentrations as high as 400 mg/l of cr(vi). moreover, ph 10 and the temperature 30°c constitute favourable conditions to the growth and reduction of acinetobacter ab1. complete reduction of cr ...201123459068
repeated colonization by multi-drug-resistant acinetobacter calcoaceticus-a. baumannii complex and changes in antimicrobial susceptibilities in surgical intensive care units.a nosocomial outbreak of multi-drug-resistant acinetobacter calcoaceticus-a. baumannii (mdr-acb) complex infection occurred in a newly constructed building at a 2,500-bed tertiary medical center in taiwan.201323425282
appraising freeze-drying for storage of bacteria and their ready access in a rapid toxicity assessment assay.direct toxicity assessment (dta) techniques seek to measure the impact of toxic chemicals on biological materials resident in the environment. this study features the use of freeze-dried bacterial cells in combination with a rapid dta analyser, scitox. the effects of three factors-cryoprotectant type, bacterial strain, and storage temperature-were tested in order to validate the shelf life of the freeze-dried cells. three freeze-dried gram-negative bacterial strains, acinetobacter calcoaceticus, ...201323377787
evolution of a pathogen: a comparative genomics analysis identifies a genetic pathway to pathogenesis in acinetobacter.acinetobacter baumannii is an emergent and global nosocomial pathogen. in addition to a. baumannii, other acinetobacter species, especially those in the acinetobacter calcoaceticus-baumannii (acb) complex, have also been associated with serious human infection. although mechanisms of attachment, persistence on abiotic surfaces, and pathogenesis in a. baumannii have been identified, the genetic mechanisms that explain the emergence of a. baumannii as the most widespread and virulent acinetobacter ...201323365658
staring at the cold sun: blue light regulation is distributed within the genus acinetobacter.we previously showed that the opportunistic nosocomial pathogen acinetobacter baumannii is able to sense and respond to light via blsa, a bluf (blue-light-sensing using fad)-domain photoreceptor protein. here, we extend our previous studies showing that light regulation is not restricted to a. baumannii, but rather widespread within the genus acinetobacter. first, we found that blue light modulates motility and biofilm formation in many species of the genus, including members of the acinetobacte ...201323358859
[production of surfactants by acinetobacter calcoaceticus k-4 grown on ethanol with organic acids].the effect of fumarate (c4-dicarboxylic acid, a gluconeogenesis precursor) and citrate (a lipid synthesis regulator) on the production of surfactants by acinetobacter calcoaceticus k-4 grown on ethanol has been studied. simultaneous addition of fumarate and citrate to concentrations of 0.01-0.02% at the end of the log phase of k-4 growth in a medium with 2 vol% ethanol increases the nominal surfactant concentration by 45-55% in comparison with a culture without organic acids. the increased level ...201323330390
antibacterial and antiplasmodial constituents of beilschmiedia cryptocaryoides.four new beilschmiedic acid derivatives, cryptobeilic acids a-d (1-4), and tsangibeilin b (5) have been isolated from the bark of beilschmiedia cryptocaryoides collected from madagascar. their structures were elucidated using detailed spectroscopic and spectrometric methods. cryptobeilic acid a (1) and tsangibeilin b (5) structures were confirmed by single-crystal x-ray diffraction analysis. compounds 1-5 displayed moderate antibacterial activity against escherichia coli 6r3, acinetobacter calco ...201323320609
characterization of rhamnolipids produced by non-pathogenic acinetobacter and enterobacter bacteria.rhamnolipid production by two non-pathogenic bacterial strains acinetobacter calcoaceticus and enterobacter asburiae, and established rhamnolipid producer pseudomonas aeruginosa was investigated. rhamnolipids were separated from supernatant and further purified by thin-layer chromatography. mass spectrometry with negative electrospray ionization revealed rhamnolipid homologues varying in chain length and unsaturation. tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid homolo ...201223313768
acinetobacter calcoaceticus-acinetobacter baumannii complex is not equal to a. baumannii. 201323295573
[effects of oil-refining microbes (genus acinetobacter) on cytogenetical structures of human lymphocytes in cell cultures].the objective of this study was to assess ability of oil-refining bacteria acinetobacter calcoaceticus and a. valentis to induce karyopathological abnormalities and chromosomal aberrations in human lymphocyte cultures. it was found that the cultures infected with a. calcoaceticus showed significantly high frequencies of cytogenetical effects and chromosomal aberrant cells as compared to the intact cultures and cultures infected with a. valentis. the most of chromosomal aberrations, mainly chroma ...201323285752
[intensification of biosurfactant synthesis by acinetobacter calcoaceticus imv b-7241 on a hexadecane–glycerol mixture ]. 201323234072
cellular fatty acids as chemical markers for differentiation of acinetobacter baumannii and acinetobacter calcoaceticus.gas chromatography (gc) was used to investigate the cellular fatty acid (cfa) composition of 141 acinetobacter baumannii and 32 a. calcoaceticus isolates from different locations in china and to find chemical markers to differentiate these two closely related bacteria.201223228842
cloning and characterization of the l-ribose isomerase gene from cellulomonas parahominis mb426.a newly isolated bacterium, cellulomonas parahominis mb426, produced l-ribose isomerase (celri) on a medium containing l-ribose as a sole carbon source. a 32 kda protein isomerizing l-ribose to l-ribulose was purified to homogeneity from this bacterium. a set of degenerated primers were synthesized based on amino acid sequences of the purified celri, and a 747 bp gene encoding celri was cloned, sequenced and overexpressed in escherichia coli. this gene encoded a 249 amino acid protein with a cal ...201323207370
serological studies on chloridazon-degrading bacteria.agglutination tests and immunofluorescence tests with antisera against four strains of chloridazon-degrading bacteria revealed the serological uniformity of a group of 22 chloridazon-degrading bacterial strains. no serological relationship could be found between chloridazon-degrading bacteria and representatives of other gram-negative bacteria. this was demonstrated by agglutination tests, including testing of the antiserum against acinetobacter calcoaceticus, and by immunofluorescence tests, in ...198323194804
degradation of aromatic carboxylic acids by acinetobacter.the degradation of the aromatic carboxylic acids 3-phenylpropionic acid, cinnamic acid and l-phenylalanine was investigated in several strains of acinetobacter calcoaceticus var. lwoffii. evidence is presented for the conversion of 3-phenylpropionic acid into the cis-2,3-dihydrodihydroxy-derivative, which is further metabolized to 3-(2,3-dihydroxyphenyl-)propionic acid, followed by cleavage of the aromatic ring in meta-proximal position. when pre-grown on 3-phenylpropionic acid, cinnamic acid as ...198323194732
comparative analysis of fecal microflora of healthy full-term indian infants born with different methods of delivery (vaginal vs cesarean): acinetobacter sp. prevalence in vaginally born infants.in this study fecal microflora of human infants born through vaginal delivery (vb) and through cesarean section (cb) were investigated using culture-independent 16s rdna cloning and sequencing approach. the results obtained clearly revealed that fecal microbiota of vb infants distinctly differ from those in their counterpart cb infants. the intestinal microbiota of infants delivered by cesarean section appears to be more diverse, in terms of bacteria species, than the microbiota of vaginally del ...201223151789
biodegradation of the herbicide propanil, and its 3,4-dichloroaniline by-product in a continuously operated biofilm reactor.the persistence of propanil in soil and aquatic environments along with the possible accumulation of toxic degradation products, such as chloroanilines, is of environmental concern. in this work, a continuous small-scale bioprocess to degrade the herbicide propanil, its main catabolic by-product, 3,4-dichloroaniline (3,4-dca), and the herbicide adjuvants is carried out. a microbial consortium, constituted by nine bacterial genera, was selected. the isolated strains, identified by amplification a ...201323117676
molecular epidemiology of acinetobacter baumannii and acinetobacter nosocomialis in germany over a 5-year period (2005-2009).to investigate the species distribution within the acinetobacter calcoaceticus-acinetobacter baumannii complex and the molecular epidemiology of a. baumannii and acinetobacter nosocomialis, 376 acinetobacter isolates were collected prospectively from hospitalized patients at 15 medical centres in germany during three surveillance studies conducted over a 5-year period. species identification was performed by molecular methods. imipenem minimum inhibitory concentrations (mic) were determined by b ...201323034071
decolorization of textile dyes and degradation of mono-azo dye amaranth by acinetobacter calcoaceticus ncim 2890.acinetobacter calcoaceticus ncim 2890 (a. caloaceticus) was found to decolorize 20 different textile dyes of various classes. decolorization of an azo dye amaranth was observed effectively (91%) at static anoxic condition, whereas agitated culture grew well but showed less decolorization (68%) within 48 h of incubation. induction of intracellular and extracellular lignin peroxidase, intracellular laccase, dichlorophenol indophenol (dcip) reductase and riboflavin reductase represented their invol ...201123024414
antibiotic resistance patterns of acinetobacter calcoaceticus-a. baumannii complex species from colombian hospitals.only automated phenotypic methods are currently used in colombian hospitals for identifying isolates of the acinetobacter calcoaceticus-a. baumannii complex (acb). the phenotypical similarities in these species mean that they cannot be differentiated by manual or automated methods, thereby leading to their identification as a. baumannii, or acb complex in clinical settings. our objective was to identify to the species level 60 isolates, from four hospitals, evaluate their antibiotic susceptibili ...201323021300
comparison of genospecies and antimicrobial resistance profiles of isolates in the acinetobacter calcoaceticus-acinetobacter baumannii complex from various clinical specimens.this study was conducted to compare the prevalences of antimicrobial resistance profiles of clinical isolates in the acinetobacter calcoaceticus-acinetobacter baumannii complex from sterile and nonsterile sites and to further study the relationship of antimicrobial resistance profiles and genospecies by amplified rrna gene restriction analysis (ardra). a total of 1,381 isolates were tested with 12 different antibiotics to show their antimicrobial susceptibility profiles. a total of 205 clinical ...201223006758
highlights on molecular identification of closely related species.the term "complex" emerged in the literature at the beginning of the genomic era associated to taxonomy and grouping organisms that belong to different species but exhibited similar patterns according to their morphological, physiological and/or other phenotypic features. dna-dna hybridization values ~70% and high identity on 16s rrna gene sequences were recommended for species delineation. electrophoretic methods showed in some cases to be useful for species identification and population struct ...201322982158
isolation and molecular identification of landfill bacteria capable of growing on di-(2-ethylhexyl) phthalate and deteriorating pvc materials.waste materials containing di-(2-ethylhexyl) phthalate (dehp), a suspected endocrine disruptor and reasonably anticipated human carcinogen, are typically disposed of in landfills. despite this, very few studies had been conducted to isolate and identify dehp-degrading bacteria in landfill leachate. therefore, this study was conducted to isolate and characterize bacteria in landfill leachate growing on dehp as the sole carbon source and deteriorating pvc materials. four strains lhm1, lhm2, lhm3 a ...201222934997
doripenem vs meropenem against pseudomonas and acinetobacter.recently, doripenem has been approved for the treatment of nosocomial pneumonia (np), including ventilator-associated pneumonia (vap). the e-test was performed to determine the mics of doripenem and meropenem in 203 endotracheal aspirate isolates that consisted of 140 acinetobacter calcoaceticus-acinetobacter baumannii complexes and 63 pseudomonas aeruginosa. doripenem showed minimum concentration necessary for inhibition of 50% (mic 50 ) of p. aeruginosa isolates at 0.38 mg/l which is several t ...201422885206
phylogenetic study of sponge associated bacteria from the lakshadweep archipelago and the antimicrobial activities of their secondary metabolites.marine ecosystem of the lakshadweep archipelago is unique and known to have a very high degree of biodiversity with a number of endemic flora and fauna. the present study focuses to isolate the endosymbiotic microorganism from sponges and its effectiveness against marine ornamental fish pathogens. the sponges were collected from agatti island of lakshadweep archipelago and identified as clathria procera, sigmadocia fibulata and dysidea granulosa. in which, 15 different types of bacteria were iso ...201122806873
recurrent bacteremia caused by the acinetobacter calcoaceticus-acinetobacter baumannii complex.this study investigated the clinical and microbiological characteristics of patients with recurrent bacteremia caused by the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex at a medical center. all acb complex isolates associated with recurrent bacteremia were identified to the genomic species level using a 16s-23s rrna gene intergenic spacer sequence-based method. genotypes were determined by the random amplified polymorphic dna patterns generated by arbitrarily primed pcr and ...201222760035
acinetobacter calcoaceticus-baumannii complex strains induce caspase-dependent and caspase-independent death of human epithelial cells.we investigated interactions of human isolates of acinetobacter calcoaceticus-baumannii complex strains with epithelial cells. the results showed that bacterial contact with the cells as well as adhesion and invasion were required for induction of cytotoxicity. the infected cells revealed hallmarks of apoptosis characterized by cell shrinking, condensed chromatin, and internucleosomal fragmentation of nuclear dna. the highest apoptotic index was observed for 4 of 10 a. calcoaceticus and 4 of 7 a ...201222684803
multitrophic interaction in the rhizosphere of maize: root feeding of western corn rootworm larvae alters the microbial community composition.larvae of the western corn rootworm (wcr) feeding on maize roots cause heavy economical losses in the us and in europe. new or adapted pest management strategies urgently require a better understanding of the multitrophic interaction in the rhizosphere. this study aimed to investigate the effect of wcr root feeding on the microbial communities colonizing the maize rhizosphere.201222629377
identification of genetic recombination between acinetobacter species based on multilocus sequence analysis.during multilocus sequence analysis of acinetobacter calcoaceticus-acinetobacter baumannii complex, we identified the evidence of recent genetic recombination between 2 acinetobacter species. while 3 isolates belonged to a. nosocomialis based on 16s rrna, gyrb, fusa, gdhb, and rplb gene sequences, they showed close relationships with acinetobacter genomic species 'close to 13tu' in rpob, reca, cpn60, rpod, and glta gene trees.201222595610
a five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by acinetobacter radioresistens sh164.acinetobacter calcoaceticus sw1, under nitrogen limitation, assimilates the nitrogen moiety of taurine (2-aminoethanesulfonate) inducibly and excretes sulfoacetaldehyde, a product of taurine dehydrogenase (tauxy). blast searches of newly available genome sequences using the tauxy sequences revealed a 5-gene cluster, taurxypi, in acinetobacter radioresistens sh164. we hypothesized that tauxypi (hmpref0018_00717-hmpref0018_00720) encodes proteins that are orthologs of the undefined pathway from st ...201222588221
diagnosis of cellulitis in the immunocompromised host.a prospective study of diagnostic techniques in cellulitis was performed on 28 patients with malignancy. twenty-two (78%) of the fine needle aspiration cultures and 10 (35%) of the blood cultures were positive in this immunocompromised population. the incidence of positive fine needle aspiration cultures (p<0.005) or bacteremia (p<0.0005) was significantly higher than results obtained in an immunocompetent population with cellulitis at the same institution. staphylococci or streptococci were rec ...199022553455
[synthesis of surfactants acinetobacter calcoaceticus imv b-7241 and rhodococcus erythropolis imv ac-5070 in the medium with glycerol].it was established that glycerol, a byproduct of biodiesel production, may be used as substrate for synthesis of surfactants rhodococcus erythropolis imv ac-5017 and acinetobacter calcoaceticus imv b-7241. maximum indices of surfactants synthesis by the strain imv b-7241 have been fixed, when the medium with glycerol included yeast autolysate and trace elements. it was shown that the surfactants synthesis could be intensified when cultivating a. calcoaceticus imv b-7241 and r. erythropolis imv a ...201322545440
domiciliary cockroaches found in restaurants in five zones of kuala lumpur federal territory, peninsular malaysia.the following domiciliary cockroaches were collected from restaurants in five zones of kuala lumpur federal territory, malaysia using 1l glass beaker traps baited with ground mouse-pellets: periplaneta americana (linnaeus) (n = 820), periplaneta brunnea burmeister (n = 46), blattella germanica (linnaeus) (n = 12504), supella longipalpa (fabricius) (n = 321), symploce pallens stephens (n = 29) and neostylopyga rhombifolia (stoll) (n = 5). the following bacteria were isolated from 10 cockroach spe ...201222543619
infections due to different genospecies of the acinetobacter calcoaceticus-a baumannii complex. 201222526214
tigecycline: an evidence-based review of its antibacterial activity and effectiveness in complicated skin and soft tissue and intraabdominal infections.there is an urgent need for novel agents to manage serious bacterial infections, particularly those contracted in healthcare facilities. tigecycline is a novel broad-spectrum glycylcycline with good activity against gram-positive, many gram-negative, anaerobic, and some atypical pathogens that has been developed to address this need.200622500153
vertical immobilization of viable bacilliform bacteria into polypyrrole films.we have successfully developed a novel technique for inserting viable bacilliform bacteria into polypyrrole films. all of the five different bacterial cells (pseudomonas aeruginosa, acinetobacter calcoaceticus, serratia marcescens, bacillus subtilis and escherichia coli) studied in this work were inserted normal to the film surface, and the viability of p. aeruginosa was unaffected by this immobilization procedure. it was also found that the polypyrrole layer was important to keep the cells aliv ...201222498456
application of amplified ribosomal dna restriction analysis in identification of acinetobacter baumannii from a tertiary teaching hospital, malaysia.acinetobacter baumannii, genomic species 3 and 13tu are being increasingly reported as the most important acinetobacter species that cause infections in hospitalized patients. these acinetobacter species are grouped in the acinetobacter calcoaceticus- acinetobacter baumannii (acb) complex. differentiation of the species in the acb-complex is limited by phenotypic methods. therefore, in this study, amplified ribosomal dna restriction analysis (ardra) was applied to confirm the identity a. baumann ...201122433885
expression of the resistance-nodulation-cell division pump adeijk in acinetobacter baumannii is regulated by aden, a tetr-type regulator.resistance-nodulation-division efflux system adeijk contributes to intrinsic resistance to various drug classes in acinetobacter baumannii. by whole-genome sequencing, we have identified in clinical isolate bm4587 the aden gene, located 813 kbp upstream from adeijk, which encodes a tetr transcriptional regulator. in one-step mutant bm4666 overexpressing adeijk, the deletion of cytosine 582 (c(582)) in the 3' portion of this gene was responsible for a frameshift mutation resulting in the deletion ...201222371895
reductive dechlorination of methoxychlor by bacterial species of environmental origin: evidence for primary biodegradation of methoxychlor in submerged environments.methoxychlor [1,1,1-trichloro-2,2-bis(4-methoxyphenyl)ethane] is an organochlorine insecticide that undergoes dechlorination in natural submerged environments. we investigated the ability to dechlorinate this compound in seven environmental bacterial species ( aeromonas hydrophila , enterobacter amnigenus , klebsiella terrigena , bacillus subtilis , achromobacter xylosoxidans , acinetobacter calcoaceticus , and mycobacterium obuense ) and the enteric bacterium escherichia coli as a positive cont ...201222292429
ecological significance of microdiversity: coexistence among casing soil bacterial strains through allocation of nutritional resource.a combination of cultivation-based methods with a molecular biological approach was employed to investigate whether bacteria with identical 16s rrna gene sequences can represent distinct eco- and genotypes. a set of eight bacterial strains wherein three were pseudomonas putida and rest were acinetobacter calcoaceticus, were isolated from casing soils community by conventional plating. these strains had identical 16s rrna gene sequences and represented the dominant phylotype in the plateable frac ...201122282622
identification of acinetobacter species: is bruker biotyper maldi-tof mass spectrometry a good alternative to molecular techniques?acinetobacter spp. has become a leading cause of nosocomial infection in recent years. phenotypic similarities between the species in the genus have made it difficult to identify them clearly using routine diagnostic methods. consequently, more relevant species have been grouped together as acinetobacter calcoaceticus-acinetobacter baumannii complex (a. baumannii, a. calcoaceticus, acinetobacter genospecies 3 and a. genospecies 13tu). however, there are other species that may also have clinical ...201222266021
clinical characteristics of tertiary hospital patients from whom acinetobacter calcoaceticus-acinetobacter baumannii complex strains were isolated.acinetobacter baumannii is a worldwide nosocomial pathogen that has become increasingly common over the past few decades, and strains of multidrug-resistant a. baumannii have been increasing. the aim of this study was to assess the clinical characteristics of a. calcoaceticus-a. baumannii complex (acb complex) strains and to determine the risk factors of this infection.201222214623
diversity of 16s-23s rdna internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors.length polymorphisms within the 16s-23s ribosomal dna internal transcribed spacer (its) have been described as stable genetic markers for studying bacterial phylogenetics. in this study, we used these genetic markers to investigate phylogenetic relationships in burkholderia pseudomallei and its near-relative species. b. pseudomallei is known as one of the most genetically recombined bacterial species. in silico analysis of multiple b. pseudomallei genomes revealed approximately four homologous r ...201122195045
Outbreak of imipenem-resistant Acinetobacter calcoaceticus-Acinetobacter baumannii complex harboring different carbapenemase gene-associated genetic structures in an intensive care unit.BACKGROUND AND PURPOSE: To investigate the clinical and molecular epidemiology of the imipenem-resistant Acinetobacter calcoaceticus-Acinetobacter baumannii (IRAcb) complex during an outbreak in an intensive care unit (ICU). METHODS: Forty-six clinical and 11 environmental isolates of the IRAcb complex were collected from the ICU of Taipei Veterans General Hospital, Taiwan between December 2003 and March 2004. These isolates were genotyped using pulsed-field gel electrophoresis (PFGE). Carbapene ...201122169123
Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria.The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ...201122163022
multifunctional acyltransferases from tetrahymena thermophila.multifunctional acyltransferases are able to catalyze the esterification of various acyl-acceptors with activated fatty acids. here we describe the identification of four proteins from tetrahymena thermophila that share certain properties with mammalian acyltransferases regarding their predicted transmembrane structure, their molecular mass and the typical acyltransferase motif. expression of the tetrahymena sequences results in production of triacylglycerols and wax esters in recombinant yeast ...201122160552
Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea.Twenty-five crude-oil-degrading bacteria were isolated from oil-contaminated sites in the Persian Gulf and the Caspian Sea. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Determination of the nucleotide sequence of the 16S rRNA gene showed that these isolated strains belonged to genera Acinetobacter, Pseudomonas, Gordonia, Rhodococcus, Cobetia, Halomonas, Alcanivorax, Marinobacter and Micro ...201222130193
identification of the naturally occurring genes encoding carbapenem-hydrolysing oxacillinases from acinetobacter haemolyticus, acinetobacter johnsonii, and acinetobacter calcoaceticus.clin microbiol infect abstract: carbapenem resistance is increasingly being reported among acinetobacter species, and results mostly from the expression of acquired carbapenem-hydrolysing oxacillinases (chdls). several acinetobacter species intrinsically possess chromosomal chdl genes: acinetobacter baumannii (bla(oxa-51) ), acinetobacter radioresistens (bla(oxa-23) ), and acinetobacter lwoffii (bla(oxa-134) ). we aimed to identify the progenitors of novel chdl-encoding genes for identification ...201122128805
diverse dna damage responses in acinetobacter include the capacity for dna damage-induced mutagenesis in the opportunistic pathogens acinetobacter baumannii and acinetobacter ursingii.error-prone and error-free dna damage repair responses that are induced in most bacteria after exposure to various chemicals, antibiotics, or radiation sources were surveyed across the acinetobacter genus. the error prone sos mutagenesis response occurs when dna damage induces a cell's umudc- or dinp-encoded error-prone polymerases. the model acinetobacter baylyi strain adp1 possesses an unusual, regulatory umud allele (umudab) with an extended 5' region, and only incomplete fragments of umuc. d ...201122117008
Comparative genomics of the type VI secretion systems of Pantoea and Erwinia species reveals the presence of putative effector islands that may be translocated by the VgrG and Hcp proteins.ABSTRACT:201122115407
a comprehensive evaluation of colonic mucosal isolates of sutterella wadsworthensis from inflammatory bowel disease.inflammatory bowel disease (ibd) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. twenty-six clinical isolates of sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had ibd. s. wadsworthensis was initially more frequently isolated from ibd subjects, hence this comprehensive study was undertaken to elucidate its ro ...201122073125
chemical, physical and biological approaches to prevent ochratoxin induced toxicoses in humans and animals.ochratoxins are polyketide derived fungal secondary metabolites with nephrotoxic, immunosuppressive, teratogenic, and carcinogenic properties. ochratoxin-producing fungi may contaminate agricultural products in the field (preharvest spoilage), during storage (postharvest spoilage), or during processing. ochratoxin contamination of foods and feeds poses a serious health hazard to animals and humans. several strategies have been investigated for lowering the ochratoxin content in agricultural prod ...201022069658
biodegradation of ochratoxin a for food and feed decontamination.ochratoxin a (ota) is one of the most important mycotoxins that is found in food and feed products. it has proven toxic properties, being primarily known for its nephrotoxicity and carcinogenicity to certain animal species. ota is produced by several species of aspergillus and penicillium that can be found in a wide variety of agricultural products, which makes the presence of ota in these products common. many countries have statutory limits for ota, and concentrations need to be reduced to as ...201022069627
ochratoxin a in ruminants−a review on its degradation by gut microbes and effects on animals.ruminants are much less sensitive to ochratoxin a (ota) than non-ruminants. the ruminal microbes, with protozoa being a central group, degrade the mycotoxin extensively, with disappearance half lives of 0.6-3.8 h. however, in some studies ota was detected systemically when using sensitive analytical methods, probably due to some rumen bypass at proportions of estimated 2-6.5% of dosage (maximum 10%). high concentrate proportions and high feeding levels are dietary factors promoting the likelines ...201022069612
integrated analysis of three bacterial conjunctivitis trials of besifloxacin ophthalmic suspension, 0.6%: etiology of bacterial conjunctivitis and antibacterial susceptibility profile.the purpose of this paper is to report on the bacterial species isolated from patients with bacterial conjunctivitis participating in three clinical trials of besifloxacin ophthalmic suspension, 0.6%, and their in vitro antibacterial susceptibility profiles.201122034556
the role of synthetic biology in the design of microbial cell factories for biofuel production.insecurity in the supply of fossil fuels, volatile fuel prices, and major concerns regarding climate change have sparked renewed interest in the production of fuels from renewable resources. because of this, the use of biodiesel has grown dramatically during the last few years and is expected to increase even further in the future. biodiesel production through the use of microbial systems has marked a turning point in the field of biofuels since it is emerging as an attractive alternative to con ...201122028591
review of glucose oxidases and glucose dehydrogenases: a bird's eye view of glucose sensing enzymes.the evolution from first-generation through third-generation glucose sensors has witnessed the appearance of a number of very diverse oxidoreductases, which vary tremendously in terms of origin, structure, substrate specificity, cofactor used as primary electron acceptor, and acceptable final electron acceptor. this article summarizes our present knowledge of redox enzymes currently utilized in commercially available glucose monitoring systems to promote a fuller appreciation of enzymatic proper ...201122027299
comparative analysis of the complete genome of an acinetobacter calcoaceticus strain adapted to a phenol-polluted environment.the complete genome sequence of acinetobacter calcoaceticus phea-2, a non-pathogenic phenol-degrading bacterium previously isolated from industrial wastewater of an oil refinery in china, has been established. this is the first sequence of an a. calcoaceticus strain. we report here a comparative genomic analysis of phea-2 with two other acinetobacter species having different lifestyles, acinetobacter baumannii aye, a pathogenic human-adapted strain, and acinetobacter baylyi adp1, a soil-living s ...201122027104
Antimicrobial resistance determinants in imipenem-nonsusceptible Acinetobacter calcoaceticus-baumannii complex isolated in Daejeon, Korea.Members of the Acinetobacter calcoaceticus-baumannii (Acb) complex are important opportunistic bacterial pathogens and present significant therapeutic challenges in the treatment of nosocomial infections. In the present study, we investigated the integrons and various genes involved in resistance to carbapenems, aminoglycosides, and fluoroquinolones in 56 imipenem-nonsusceptible Acb complex isolates.201122016680
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