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pathogenic significance of acinetobacter calcoaceticus: analysis of experimental infection in mice.the phenomenon that mixed infection with certain species of bacteria and acinetobacter calcoaceticus is more virulent than single infection was analyzed experimentally. in mixed infections with a. calcoaceticus paired with either escherichia coli, serratia marcescens, or pseudomonas aeruginosa, the virulence of the latter three organisms was markedly increased over that of single infections only by slime-producing strains of a. calcoaceticus. of the 100 strains of a. calcoaceticus tested, 14 had ...19863773789
[diffuse growth of strains of acinetobacter calcoaceticus on blood agar and their motility digging appearance on soft-agar medium]. 19863773266
[aerobic treatment of pig slurry: microbial aspects].during the treatment of raw pig slurry by batch aerobic fermentation, the presence of surfaces for bacterial attachment resulted in a 1,000-fold increase in the microbial population. in the absence of such surfaces, a 65% reduction of the chemical oxygen demand (cod) of the manure was obtained after 168 h of aeration, whereas a value of 90% was observed in their presence. in the early stages of the treatment, during which the redox potential passed from a value characteristic of anaerobic condit ...19863768808
emulsifier formation with acinetobacter: search for an excretion-reduced mutant of acinetobacter calcoaceticus 69-v.after growth on acetate three groups of acinetobacter strains could be identified with respect to the excretion of a bioemulsifier. mutants of a. calcoaceticus 69-v were selected which produced reduced amounts of emulsifier.19863746629
variation in composition and yield of exopolysaccharides produced by klebsiella sp. strain k32 and acinetobacter calcoaceticus bd4.the exopolysaccharides produced by klebsiella sp. strain k32 and acinetobacter calcoaceticus bd4 under different growth conditions have been analyzed for sugar composition. the first use of ion chromatography for the quantitative determination of microbial exopolysaccharide composition is reported. klebsiella sp. strain k32 produced a polymer composed of rhamnose, galactose, and mannose early in its fermentation. the composition of the polymer varied markedly depending on the growth stage of the ...19863729401
acinetobacter calcoaceticus in three cases of late abortion in water buffaloes. 19863716168
[temperature dependence of membrane-bound aldehyde dehydrogenase from acinetobacter: effect of solubilization and chain length of the substrates].the nadp+-dependent aldehyde dehydrogenase of intracytoplasmic membranes of acinetobacter calcoaceticus shows a characteristic behaviour of its reaction velocities in dependence on temperature. the calculated "real" activation energies depend on the increasing chain-length of the homologous aldehydes and grow appropriate to the membrane-bound and to the micellar form of the enzyme with 6.2 and 7.3 kj/mole ch2-group, respectively. the values of the activation energy of the membrane-bound enzyme a ...19863707551
evolved aniline catabolism in acinetobacter calcoaceticus during continuous culture of river water.adaptation of acinetobacter calcoaceticus from river water to aniline depends on the dynamics of parent and mutant populations. the parent, acinetobacter strain don26 phenotype ani0, was common in river water and assimilated aniline effectively at micromolar concentrations, but was inhibited at higher concentrations of aniline. the ani0 phenotype was also characterized by a broad specificity for oxidation of chloroanilines by aniline-induced cells. the mutant ani+ phenotype was represented by do ...19863707123
isolation and identification of a rhamnosyl-rhamnosyl-3-deoxy-d-manno-octulosonic acid trisaccharide from the lipopolysaccharide of acinetobacter calcoaceticus (10303 ntcc london). 19873690574
drag reduction by acinetobacter calcoaceticus bd4.the encapsulated bacterium acinetobacter calcoaceticus bd4 at a density of 3.6 x 10(9) cells per ml reduced the friction of turbulent water in a narrow pipe by 55%. this drag reduction was due to the tightly bound polysaccharide capsules (0.4 mg per ml) of culture. capsule-deficient mutants of bd4 failed to reduce drag. the cell-bound polysaccharide demonstrated a threefold-higher drag-reducing activity than the polymer which was free in solution.19873674875
acinetobacter calcoaceticus infection following a dog bite.a frequent complication of dog bite wounds is bacterial infection. the choice of antibiotics is based upon the most likely organisms. failure to achieve wound healing suggests that an uncommon organism(s) is present and should lead the clinician to culture the wound. a case of acinetobacter calcoaceticus infection following a dog bite is described.19873668199
chronic acinetobacter calcoaceticus var anitratus pneumonia.gram-negative bacteria most often affect the lung in an acute, suppurative process; however, these organisms may also produce chronic pneumonia. acinetobacter calcoaceticus has not been reported previously as a cause of chronic pneumonia. we present a patient with fatal, chronic community-acquired acinetobacter pneumonia with chest wall invasion at autopsy.19873631123
a simple spectrophotometric assay for arogenate dehydratase.a simple spectrophotometric assay for arogenate dehydratase, the enzyme that catalyzes the formation of l-phenylalanine from l-arogenate, is presented. the method couples the arogenate dehydratase reaction with that of an aromatic aminotransferase partially purified from acinetobacter calcoaceticus. in the presence of 2-ketoglutarate, phenylpyruvate formation is measured at 320 nm at basic ph. the method was compared with two other methods already in use in our laboratory for arogenate dehydrata ...19873619011
cell envelope protein profiles of acinetobacter calcoaceticus strains isolated in hospitals.the cell envelope protein patterns of 78 strains of acinetobacter calcoaceticus, mainly isolated in hospitals, were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). the patterns were stable and reproducible. comparison of the protein profiles made possible differentiation between two groups of strains. the patterns of the first group could be classified on the basis of concordance. some profiles appeared to be associated with the epidemiological origin of the st ...19873585964
effect of oxygen limitation on the content of n-hexadecane-inducible cytochrome p-450 in acinetobacter calcoaceticus strain eb 104.the content of cytochrome p-450 as a function of oxygen supply was studied during growth of acinetobacter on n-hexadecane in batch cultures at constant ph and agitation. the rate of growth and the content of cytochrome p-450 were not affected as long as the dissolved oxygen tension ranged above 3 to 5% of saturation. the amount of cytochrome p-450 increased when the oxygen tension declined to zero. cytochrome p-450 levels of about 0.3 to 0.4 nmol/mg protein, i.e. a more than a threefold increase ...19863585713
prosthetic valve endocarditis caused by acinetobacter calcoaceticus subsp. lwoffi.acinetobacter spp. are uncommon etiologic agents of prosthetic valve endocarditis. two patients with acinetobacter calcoaceticus subsp. lwoffi prosthetic valve endocarditis are described. the patients were successfully treated with antibiotics (cefotaxime sodium and gentamicin sulfate); thus, we suggest medical treatment rather than early valve replacement in this particular type of infection.19873584434
uv-inducible dna repair in acinetobacter calcoaceticus.bacterial mutation frequency after uv irradiation and phage mutation frequency under conditions of w-reactivation were determined in a. calcoaceticus. with the exception of streptomycin resistance, there was no increase in the frequency of the assayed markers above the background level. the increased survival of phage during w-reactivation was not followed by an increase in the frequency of mutation from turbid to clear plaque formers among phage survivors. the findings suggested that the uv-ind ...19873574328
ultracytochemical localization of aldehyde dehydrogenase in acinetobacter calcoaceticus.a membrane-bound aldehyde dehydrogenase is induced in acinetobacter calcoaceticus grown on aliphatic hydrocarbons as sole carbon source. this enzyme is nadp-dependent and is able to oxidize medium- and long-chain aliphatic aldehydes to their corresponding fatty acids. electron micrographs of sectioned alkane-adapted bacteria showed hydrocarbon inclusions in the cytoplasmic matrix. the cytochemical phenazine methosulphate-tetranitro tetrazolium blue capture reaction allowed to localize the activi ...19863572743
defensins mediate the microbicidal activity of human neutrophil granule extract against acinetobacter calcoaceticus.an acid extract of human neutrophil granules was fractionated on a sephadex g-100 column and tested for microbicidal activity against acinetobacter calcoaceticus ho-1 as described previously (m.c. modrzakowski and c. m. paranavitana, infect. immun. 32:668-674, 1981). the low-molecular-weight protein fraction, peak d, accounted for about 30% of the protein and nearly all of the activity of the crude extract against strain ho-1. peak d protein proved to be a mixture of the three human defensin pep ...19873570467
[kinetics of membrane-bound aldehyde dehydrogenase from acinetobacter calcoaceticus].in recent investigations we were able to demonstrate that the nadp-dependent aldehyde dehydrogenase of acinetobacter calcoaceticus is an inducible enzyme localized in intracytoplasmic membranes limiting alkane inclusions. long-chain aliphatic hydrocarbons and alkanols are inducers of the enzyme. it was purified by us and now kinetically characterized using the enzyme-micelle form, which contains bacterial phospholipids and a detergent (sodium cholate), too. the ph optimum of aldehyde dehydrogena ...19873566912
reconstitution of emulsifying activity of acinetobacter calcoaceticus bd4 emulsan by using pure polysaccharide and protein.acinetobacter calcoaceticus bd4 and bd413 produce extracellular emulsifying agents when grown on 2% ethanol medium. for emulsifying activity, both polysaccharide and protein fractions were required, as demonstrated by selective digestion of the polysaccharide with a specific bacteriophage-borne polysaccharide depolymerase, deproteinization of the extracellular emulsifying complex with hot phenol, and reconstitution of emulsifier activity with pure polysaccharide and a polysaccharide-free protein ...19873566271
the epidemiology of acinetobacter calcoaceticus: biotype and resistance-pattern of 328 strains consecutively isolated from clinical specimens.328 clinical isolates of acinetobacter calcoaceticus from hospitalized patients and persons attending general practitioners were characterized according to biotype and resistance-pattern. 117 strains of biotype (b.) anitratus, 200 of b.lwoffi, 11 of b. haemolyticus and no strains of b. alcaligenes were found. b. anitratus was more frequently isolated from patients in hospitals than b.lwoffi; b.lwoffi was more often found in general practice. strains of b. anitratus had more resistance traits tha ...19873565019
evaluation of enzyme immunoassay (chlamydiazyme) for detecting chlamydia trachomatis in genital tract specimens.an enzyme immunoassay (chlamydiazyme) for detecting chlamydia trachomatis was evaluated on genital specimens from 96 men and 272 women attending a clinic for sexually transmitted diseases (std clinic). compared with a direct immunofluorescence test for chlamydial elementary bodies, the enzyme immunoassay had a sensitivity of 58% on specimens from men, a specificity of 90%, a positive predictive value of 93%, and a negative predictive value of 88%; the assay had a sensitivity of 67% on specimens ...19873546397
postantibiotic effect of imipenem on gram-positive and gram-negative micro-organisms.the bactericidal effect (be) of an antibiotic reduces the infective population, and its postantibiotic effect (pae) assures a persistent inhibition of bacterial cells after a short exposure to the antimicrobial agent. both effects prevent the early regrowth of the infecting organisms when the antibiotic tissue levels decrease to below the mic value. the be and the pae of imipenem, cefotaxime, ceftazidime, piperacillin, gentamicin and ampicillin on escherichia coli, klebsiella pneumoniae, enterob ...19863546247
comparative activity of imipenem, ceftazidime and cefotaxime against acinetobacter calcoaceticus.imipenem was the most active drug against acinetobacter, even against strains possessing beta-lactamases. mics of imipenem for 50% and 90% of the a. calcoaceticus (var. anitratum) were 0.28 and 0.61 mg/l respectively whereas for ceftazidime they were 4.4 and 10.0, and for cefotaxime, 7.9 and 22.2 mg/l. no change occurred in the in-vitro activity of imipenem against acinetobacter, during the period 1980-1985. geometric mean mics in 1985 were 0.33 mg/l for a. calcoaceticus var. anitratum; the valu ...19863546246
cloning of the genes involved in synthesis of coenzyme pyrrolo-quinoline-quinone from acinetobacter calcoaceticus.mutants of acinetobacter calcoaceticus lmd79.41 were isolated that are defective in the synthesis of the coenzyme pyrrolo-quinoline-quinone (pqq). a gene bank of the wild-type. a. calcoaceticus genome was constructed with the binary plasmid system plv21-rp4 delta km. the dna of a. calcoaceticus lmd79.41 was partially digested with sau3a, and fragments of about 15 kilobases were inserted into the bamhi site of plv21. the hybrid plasmids maintained in escherichia coli were transferred by conjugati ...19873539922
cloning and expression of acinetobacter calcoaceticus catechol 1,2-dioxygenase structural gene cata in escherichia coli.catechol 1,2-dioxygenase (ec 1.13.1.1), the product of the cata gene, catalyzes the first step in catechol utilization via the beta-ketoadipate pathway. enzymes mediating subsequent steps in the pathway are encoded by the catbcde genes which are carried on a 5-kilobase-pair (kbp) ecori restriction fragment isolated from acinetobacter calcoaceticus. this dna was used as a probe to identify escherichia coli colonies carrying recombinant puc19 plasmids with overlapping sequences. repetition of the ...19863536862
[comparative activity in vitro of ceftizoxime, ceftazidime and imipenem against acinetobacter calcoaceticus].acinetobacter calcoaceticus, a nosocomial pathogenic agent, is isolated with increasing frequency from hospitalized patients. acinetobacter is one of the most resistant pathogens to currently available antibiotics, particularly beta-lactam antibiotics. beta-lactamases (tem penicillinase and cephalosporinase) and problems of permeability are the most frequent mechanisms of resistance. the authors compared the in vitro activity of ceftizoxim, ceftazidim and imipenem against 82 clinical isolates of ...19863534756
[in vitro study of the pefloxacine-ceftriaxone combination against 18 strains of acinetobacter].the bacteriostatic and bactericidal activity of the pefloxacin-ceftriaxone combination was studied in mueller-hinton broth against 18 clinical strains of acinetobacter calcoaceticus var. anitratum by the checkerboard technique. the bactericidal activity was tested after 2, 4, 6 and 24 hours contact. mics were 0.12 to 4 mg/l for pefloxacin and 2 to 64 mg/l for ceftriaxone. the bacteriostatic interaction of the antibiotics was synergistic for 5 strains (fic index = 0.375 to 0.5) and additive for 1 ...19863534716
cloning and expression of the acinetobacter calcoaceticus mutarotase gene in escherichia coli.this article describes the cloning of the mutarotase gene from acinetobacter calcoaceticus and its expression in escherichia coli. purification of mutarotase (ec 5.1.3.3) led to a single polypeptide of 40 kilodaltons. the sequences of 27 n-terminal and 76 c-terminal amino acids were determined. from six amino acids of the n-terminal and seven amino acids of the c-terminal portion of the protein, the sequences of two oligonucleotides were deduced. these were synthesized and used as gene probes. c ...19863531172
an infant with simultaneous beta-lactamase-positive haemophilus influenzae meningitis and beta-lactamase-negative h. influenzae septicemia, escherichia coli pyelonephritis and herpes encephalitis.we describe an infant with meningitis and septicemia due to infection with two different strains of haemophilus influenzae, with a urinary tract infection due to escherichia coli and in whom herpes virus encephalitis was diagnosed within three days. acinetobacter calcoaceticus septicemia developed three weeks later. no immunological deficiency could be demonstrated in the patient who recovered finally, albeit with sequelae due to encephalitis.19863524107
[acinetobacter calcoaceticus strain with a wide spectrum of utilizing aromatic compounds and carrying a plasmid for resorcin degradation].a bacterial strain was isolated from soil and identified as acinetobacter calcoaceticus var. lwoffii. the strain can utilize a wide spectrum of aromatic compounds. it carries a transmissive plasmid pbsw13 which determines resorcin utilization via the ortho pathway including the following steps: resorcin-hydroxyhydroquinone-maleylacetate-beta-ketoadipi c acid. the plasmid has been transferred by conjugation into the recipient strains of a. calcoaceticus 5734 ccm rifr, escherichia coli j-53 met-pr ...19863523168
[cell envelope-bound proteinase activities of acinetobacter calcoaceticus].acinetobacter calcoaceticus contains proteolytic activity after growth on various culture media. the enzyme activity could be found in the cytosolic fraction as well as in the cell envelopes (also containing the intracytoplasmic membranes). the highest proteolytic activity could be detected during the transition from the logarithmic growth phase to the stationary phase and in the early stationary phase, respectively. in the culture medium proteolytic activity was only evident in the later statio ...19863518708
leucine aminopeptidase in intracytoplasmic membranes of acinetobacter calcoaceticus.cells of acinetobacter calcoaceticus strain 69-v contain an aminopeptidase that cleaves l-leucine amide, leucylglycine or leucine hydrazide with high efficiency. leucine 4-nitroanilide and hydrazide are hydrolyzed to less than 0.1% and 1%, resp. of leucine amide. grown on acetate-nh4+ medium the activity of the enzyme in the cytoplasm is increased 5-fold compared with cells grown on a casamino acid medium or on yeast extract. in these cases the specific activity of the unpurified enzyme is about ...19873503925
isolation and characterization of a prokaryotic sulfurtransferase.a sulfurtransferase has been purified to apparent homogeneity from the prokaryote acinetobacter calcoaceticus lwoffi by conventional protein fractionation techniques. steady-state kinetic studies of the enzyme revealed that its formal mechanism varies with the acceptor substrate employed. with inorganic thiosulfate as the sulfane sulfur-donor substrate and cyanide anion as the acceptor, the enzyme was shown to catalyze the reaction by a double displacement mechanism like that of mammalian rhodan ...19873480285
hospital acquired native valve endocarditis caused by acinetobacter calcoaceticus and treated with imipenem/cilastin.a case of hospital acquired endocarditis due to acinetobacter calcoaceticus in a severely burned patient is presented. both aortic and mitral native valves were affected and the organism was resistant to most antimicrobial agents.19873479562
in vitro and in vivo activity of ny-198, a new difluorinated quinolone.ny-198 [1-ethyl-6,8-difluoro-1,4-dihydro-7-(3-methyl-1-piperazinyl)-4-oxo-3- quinolinecarboxylic acid hydrochloride] is a new difluorinated quinolone characterized by the presence of a c-methyl group at the 3 position of the piperazine moiety. it has a broad antibacterial spectrum. the in vitro antibacterial activity of ny-198 was almost the same as those of ofloxacin and norfloxacin, but far exceeded that of pipemidic acid. ny-198 was more active than norfloxacin against pseudomonas maltophilia ...19873476021
influence of the catbce sequence on the phenotypic reversion of a pcae mutation in acinetobacter calcoaceticus.isofunctional beta-ketoadipate:succinyl coenzyme a transferases i and ii are encoded by the pcae and cate genes, respectively, of acinetobacter calcoaceticus. the genes are under separate transcriptional control and genetically unlinked. mutations in the pcae gene result in a p-hydroxybenzoate-negative (pob-) phenotype, whereas cate mutations cause a benzoate-negative (ben-) phenotype. a. calcoaceticus adp125 carries the pcae3125 mutation and gave rise to pob+ revertants with a frequency of 10(- ...19873474227
in vitro activity of antimicrobial agents against acinetobacter calcoaceticus.the aim of this study was to evaluate the in vitro activity of several new microbial agents against 96 acinetobacter calcoaceticus isolates. among several new beta-lactams, imipenem, a new, broad-spectrum, highly potent penem, was the most active drug in vitro against these strains, with a geometric mean mic of about 0.3 mg/l. ceftazidime and ceftizoxime also demonstrated good in vitro activity with geometric mean mics of 6 mg/l and 7 mg/l respectively. among new quinolones, ciprofloxacin, oflox ...19863471431
in-vitro antibacterial activity of imipenem compared with four other beta-lactam antibiotics (ceftazidime, cefotaxime, piperacillin and azlocillin) against 828 separate clinical isolates from a portuguese hospital.an agar dilution technique was used to determine the mic50 and mic90 of imipenem in comparison with ceftazidime, cefotaxime, piperacillin and azlocillin. eight hundred and eighteen of the 828 (98.8%) unselected gram-positive cocci and gram-negative bacilli were inhibited by imipenem at a concentration of 8 mg/l or less. imipenem was shown to be superior to the other four beta-lactam antibiotics against acinetobacter calcoaceticus (40 strains), all the staphylococcus aureus (80 strains, 10 of the ...19863469195
susceptibility of non-fermentative gram-negative bacteria to ciprofloxacin, norfloxacin, amifloxacin, pefloxacin and cefpirome.susceptibility of 340 mainly clinically isolated gram-negative non-fermenters to ciprofloxacin, norfloxacin, amifloxacin, pefloxacin and cefpirome was determined by agar dilution. ciprofloxacin was most active, with mic90s against all organisms ranging between less than 0.125 and 4 mg/l. norfloxacin, amifloxacin and pefloxacin were active against most strains, with mic90 ranges (mg/l) of 0.5 - 32, 0.25 - 32 and less than 0.125 - 16, respectively. cefpirome showed less activity than the quinolone ...19863469178
antimicrobial activity of imipenem against 1386 clinical isolates.1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...19863468319
acinetobacter calcoaceticus sepsis in children with malignancies.the clinical and microbiologic characteristics of 29 episodes of sepsis caused by acinetobacter calcoaceticus were reviewed in 25 children with underlying malignancies. of the 29 episodes of sepsis with this organism 28 occurred from 1980 through 1984, compared with 1 episode from 1973 to 1979. risk of infection was associated with the presence of intravascular cannulae, osteosarcoma and recent administration of antitumor chemotherapy. there was no association with neutropenia, malnutrition or f ...19863463939
quinoprotein glucose dehydrogenase and its application in an amperometric glucose sensor.glucose dehydrogenase (gdh), one of the recently discovered nad(p)+-independent 'quinoprotein' class of oxidoreductase enzymes, was purified from acinetobacter calcoaceticus lmd 79.41 and immobilised on a 1,1'-dimethylferrocene-modified graphite foil electrode. the second-order rate constant (ks) for the transfer of electrons between gdh and ferrocenemonocarboxylic acid (fmca) in a homogeneous system, determined using direct current (dc) cyclic voltammetry, was found to be 9.4 x 10(6) litres mol ...19863454651
isolation and characterization of the extracellular lipase of acinetobacter calcoaceticus 69 v.the extracellular lipase of acinetobacter calcoaceticus 69 v was purified by hydrophobic interaction chromatography to homogeneity as suggested by gel electrophoretic analysis. the lipase existed as a high molecular complex of about 300 kda, with a subunit molecular weight of 30.5 kda being obtained by sds-page. the hydrodynamic molecular radius obtained by gel electrophoresis was 3.27 nm. the lipase had an isoelectric point of 5.5 and was stimulated by additions of deoxycholate. the activation ...19873453402
pww174: a large plasmid from acinetobacter calcoaceticus encoding benzene catabolism by the beta-ketoadipate pathway.acinetobacter calcoaceticus rje74 contains a large transmissible catabolic plasmid, pww174, of about 200 kb, which encodes its ability to grow on benzene (bzn+). pww174 was unstable in acinetobacter hosts and was lost at high frequency in the absence of selection for bzn+. the catabolic pathway appeared to be via benzene cis-glycol, catechol and the beta-ketoadipate (ortho) pathway. pww174 encodes a catechol 1,2-oxygenase which is significantly more thermolabile than the chromosomally determined ...19873449741
[cytochrome p-450 and the respiratory activity of acinetobacter calcoaceticus growing on n-nonane]. 19873448466
bacterial flora in bottled uncarbonated mineral drinking water.a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...19873446349
enzymatic resistance to beta-lactams and aminoglycosides in acinetobacter calcoaceticus. 19873440769
[antibacterial activity of species of the genus allium].garlic, onion and shallot were tested for antimicrobial activity against pathogenic aerobic and anaerobic bacteria. the mic of aqueous and petroleum ether extracts were determined. garlic showed the greater activity; the combination garlic-antibiotic is synergistic against acinetobacter calcoaceticus and leads to indifference against anaerobic bacteria. the active constituent is not probably allicin alone.19873438321
protein fingerprinting for the determination of relatedness in acinetobacter calcoaceticus subspecies anitratus isolated from patients in a surgical intensive care unit.the use of protein fingerprinting for the establishment of relatedness among isolates of acinetobacter calcoaceticus subspecies anitratus, isolated from patients in a surgical intensive care unit was examined. polyacrylamide gel electrophoresis was used to analyze the cellular proteins from whole cell lysates of 14 intensive care unit a calcoaceticus subspecies anitratus isolates and 11 control strains. antimicrobial susceptibilities and plasmid profiles were also determined for all isolates. al ...19873429162
typing of acinetobacter calcoaceticus strains isolated from hospital patients by cell envelope protein profiles.the usefulness of sodium dodecyl sulphate-polyacrylamide gel electrophoresis patterns of cell envelope proteins for classifying strains of acinetobacter calcoaceticus was studied using 129 isolates from 16 in-patients in a teaching hospital. in 11 patients, all of the isolates from each patient exhibited the same pattern irrespective of the body site or time of isolation. the patterns of the isolates from four other patients were indistinguishable, with the exception of one isolate per patient. ...19873428372
effect of plasmid rp1 on phase changes in inner and outer membranes and lipopolysaccharide from acinetobacter calcoaceticus: a fourier transform infrared study.the successful transfer of the resistance plasmid rp1 into the gram-negative bacterium acinetobacter calcoaceticus resulted in increased resistance of this microorganism to the antibiotics kanamycin and tetracycline. microorganisms harboring the rp1 plasmid showed altered fatty acid composition in the lipopolysaccharide fraction and increased outer membrane permeability compared to organisms without the plasmid. thermotropic gel to liquid crystal lipid phase changes were detected in both inner a ...19873427032
nosocomial respiratory tract colonization and infection with aminoglycoside-resistant acinetobacter calcoaceticus var anitratus: epidemiologic characteristics and clinical significance.during the period july 1983 through december 1984, aminoglycoside-resistant acinetobacter calcoaceticus var anitratus (aca) were isolated from 98 patients in a university hospital. eighty-seven percent of patients (85/98) acquired aminoglycoside-resistant aca in the intensive care unit (icu) and 92% (90/98) of all initial isolates were from sputum. icu patients with respiratory colonization/infection with aminoglycoside-resistant aca were compared with matched icu controls with other gram-negati ...19883403939
nucleotide sequence of the gene coding for quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus. 19883399393
community-acquired acinetobacter pneumonia in adults in papua new guinea.acinetobacter calcoaceticus, an aerobic gram-negative coccobacillus, is a rare cause of community-acquired pneumonia. it most commonly causes nosocomial infections, particularly in elderly debilitated patients who have undergone surgery, instrumentation, and antibiotic therapy. in a study of acute pneumonia in adults, five cases of community-acquired acinetobacter pneumonia were observed over an 11-month period. other than chronic pulmonary disease (two patients), no serious underlying diseases ...19883393786
criteria for disk susceptibility tests and quality control guidelines for the cefoperazone-sulbactam combination.for in vitro susceptibility tests with cefoperazone and sulbactam (a beta-lactamase inhibitor), 75/30-micrograms disks may be used with the interpretive zone size breakpoints that are currently used for 75-micrograms cefoperazone disks. for dilution tests, a 2:1 ratio of cefoperazone to sulbactam is recommended. for quality control purposes, mic limits that are used to monitor cefoperazone tests were also applied to tests with the combination of drugs. for gram-negative control strains, zone siz ...19883343304
purification and properties of l-mandelate dehydrogenase and comparison with other membrane-bound dehydrogenases from acinetobacter calcoaceticus.l-mandelate dehydrogenase was purified from acinetobacter calcoaceticus by triton x-100 extraction from a 'wall + membrane' fraction, ion-exchange chromatography on deae-sephacel, (nh4)2so4 fractionation and gel filtration followed by further ion-exchange chromatography. the purified enzyme was partially characterized with respect to its subunit mr (44,000), ph optimum (7.5), pi value (4.2), substrate specificity and susceptibility to various potential inhibitors including thiol-blocking reagent ...19873325042
[proteases in different membrane fractions of acinetobacter calcoaceticus].distinct protease activities were found in membrane fractions from acinetobacter calcoaceticus grown on acetate-nh4+ medium until early stationary phase. mechanical or enzymatic cell disintegration followed by membrane fractionation through sucrose gradient revealed higher activities in the outer membrane than in the cytoplasmic membrane. using azocasein and synthetic p-nitroanilides as substrates we found very low proteinase activities in intracytoplasmic membrane fractions. however, these frac ...19873309254
[evaluation of the atb system and the 32 gn tests for the identification and determination of the antibiotic sensitivity of acinetobacter calcoaceticus var. anitratum. comparison with the reference dilution method in a gel medium].the authors determined the susceptibility to 24 antibiotics of 95 strains of acinetobacter calcoaceticus variety anitratum with the automated api atb system in comparison with the agar dilution method for mic measurement. the strains have been identified with api 32 gn (automated system) and api ne. there was a good agreement between the two sets of identification results. in susceptibility tests the discrepancies ranged from 0.86% to 3% with a mean value of 1.8%. the overall agreement was 95% b ...19873302861
detection of the cofactor pyrroloquinoline quinone.in order to demonstrate the presence or absence of a pyrroloquinoline quinone (pqq) synthesizing capacity in microorganisms, we have found that media and equipment must be treated to remove contaminating pqq. procedures are described which appear to be effective for that purpose. these have been used with acinetobacter calcoaceticus pqq- strains to develop a sensitive and reliable assay for pqq. they also have been used to show that under our conditions of growth escherichia coli does not synthe ...19873300411
[selection of mutants of microorganisms utilizing ethanol].mutants of the bacteria acinetobacter calcoaceticus 34 and acinetobacter sp. 172 as well as of the yeast candida requinyii 316 resistant to acetaldehyde grow better in a medium with ethanol than their parent cultures. in their specific growth rate and alcohol dehydrogenase activity, 28.7-66.7% of such mutants are superior to any clone isolated in a non-selective medium. a medium containing ethanol and acetaldehyde (0.5 to 1.0% by volume) is proposed to select and isolate highly productive mutant ...19873295493
comparative in vitro activity of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone against hospital strains of gram-negative and -positive bacteria.the in vitro antibacterial activities of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone were compared against 330 gram-negative and gram-positive strains isolated from clinical specimens received at the king abdulaziz university hospital (kauh) in saudi arabia. the antibacterial susceptibility was determinated by stokes method and by the minimal inhibitory concentration (mic) using an agar dilution method. ceftazidime and ceftriaxone were the most active antibiotics, inhibiting ...19883293818
production of a phosphatidylglycerol-like substance by genital flora bacteria.bacteria contaminating amniotic fluid have anecdotally been shown to produce a phosphatidylglycerol-like substance, thus giving false positive tests for phosphatidylglycerol in vaginal pool fluid from patients with premature rupture of the membranes. isolates of escherichia coli, proteus mirabilis, acinetobacter calcoaceticus, klebsiella pneumoniae, staphylococcus aureus, coagulase-negative staphylococcus, streptococcus faecalis, and streptococcus agalactiae (group b streptococcus) were grown in ...19883293449
benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus. purification and preliminary characterization.a quick, reliable, purification procedure was developed for purifying both benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from a single batch of acinetobacter calcoaceticus n.c.i.b. 8250. the procedure involved disruption of the bacteria in the french pressure cell and preparation of a high-speed supernatant, followed by chromatography on deae-sephacel, affinity chromatography on blue sepharose cl-6b and matrex gel red a, and finally gel filtration through a superose 12 fast-prot ...19883291854
distribution of beta-lactamases and phenotype analysis in clinical strains of acinetobacter calcoaceticus.one hundred clinical strains of acinetobacter calcoaceticus isolated from 1981 to 1986 were screened for enzymatic resistance to beta-lactam antibiotics. fourteen beta-lactam antibiotics were tested and four phenotypes were defined on the basis of enzymatic resistance and of susceptibility to the following beta-lactams: ticarcillin, piperacillin, cefotaxime, and ceftazidime. the resistance of a. calcoaceticus to beta-lactam antibiotics was predominantly due to beta-lactamases, which were produce ...19883264830
occurrence, transfer and mobilization in epilithic strains of acinetobacter of mercury-resistance plasmids capable of transformation.a 7.8 kb plasmid (pqm17) encoding mercury resistance was isolated from two epilithic strains of acinetobacter calcoaceticus. the plasmid had a broad host range when mobilized by rp1, transferring into pseudomonas aeruginosa, p. putida, p. fluorescens, escherichia coli, proteus vulgaris and chromobacterium sp. with frequencies ranging from 5.3 x 10(-9) to 4.6 x 10(-4) per recipient. the plasmid could be transferred into a. calcoaceticus bd413 using intact cells of donor and recipient bacteria (i. ...19883254940
[the synthesis of group b vitamins under various conditions for the cultivation of acinetobacter calcoaceticus on ethanol media]. 19883249582
[hemolytic properties of acinetobacter calcoaceticus strains]. 19883246922
effect of hexadecane-induced vesiculation on the outer membrane of acinetobacter calcoaceticus.lipopolysaccharide-rich vesicles were released from acinetobacter calcoaceticus 69v during growth on hexadecane. vesicle formation occurred over the whole surface of the cell as demonstrated by scanning electron microscopy. in contrast, the surface of acetate-grown cells, for which little lipopolysaccharide was found in the growth medium, appeared smooth. the overall chemical composition as well as the protein and phospholipid composition of the outer membranes of both cell types was very simila ...19883246592
antimicrobial activity of mdl 19,592: an oral cephalosporin.mdl 19,592, a semisynthetic oral cephalosporin, has an antimicrobial spectrum principally directed against gram-positive cocci (mic50, 0.25-4 micrograms/ml), branhamella catarrhalis (mic50, 1-2 micrograms/ml), and pathogenic neisseria spp. such as meningococci (mic50, 4 micrograms/ml). enterobacteriaceae, enterococci, pseudomonas spp., corynebacterium jeikeium, and acinetobacter calcoaceticus strains had mdl 19,592 mic50 of greater than or equal to 32 micrograms/ml. although mdl 19,592 generally ...19883246143
[antibiotic sensitivity of nonfermenting gram-negative bacteria].antibiotic resistance of 132 strains of nonfermenting gram-negative bacteria (ngnb) was studied. 43, 20, 17, 14 and 12 of them belonged to acinetobacter calcoaceticus (anitratus and lwoffi), pseudomonas cepacia, alcaligenes faecalis, p. stutzeri and p. maltophilia, respectively. with rare exceptions all the strains were resistant to benzylpenicillin, oxacillin, lincomycin, ampicillin and cephaloridine. sensitivity to the other antibiotics varied within wide ranges. amikacin (94.3 per cent) and t ...19883228322
[acinetobacter calcoaceticus: epidemiologic aspects and distribution in the natural and hospital environment]. 19883226936
community-acquired bacteremic acinetobacter calcoaceticus var. anitratus pneumonia. 19883215692
[utilization of 3-chlorobenzoic acid by a mixed culture of microorganisms].a mixed microbial culture (acinetobacter calcoaceticus inmi-kz-3 and alcaligenes faecalis inmi-kz-5) completely utilises 3-chlorobenzoic acid (3-cba), and the liberation of chlorine atoms is 100% of the theoretically possible one. a. faecalis growth in the mixed culture at the account of 2-chloro-cis,cis-muconic acid which is accumulated in the course of 3-cba metabolism by a. calcoaceticus inmi-kz-3.19883211007
[characteristics of acinetobacter sp. strains isolated from a hospital environment. iii. anitracinogenic properties of acinetobacter calcoaceticus isolated from clinical specimens and other sources]. 19883210835
taxonomic studies of acinetobacter species based on the electrophoretic analysis of enzymes.thirty six strains of acinetobacter, 22 strains from the culture collections and 14 isolates from soil and cotton samples, were examined for the presence of twelve enzymes using polyacrylamide gel electrophoresis. a numerical analysis was carried out on the basis of similarity values obtained from the electrophoretic mobilities of the enzymes. the strains used were divided into four clusters (z-1, z-2, z-3 and z-4), and cluster z-1 was further divided into three subclusters (z-1a, z-1b and z-1c) ...19883210151
isolation of acinetobacter calcoaceticus from septicaemic hens. 19883195030
multiple intensive care unit outbreak of acinetobacter calcoaceticus subspecies anitratus respiratory infection and colonization associated with contaminated, reusable ventilator circuits and resuscitation bags.acinetobacter calcoaceticus subspecies anitratus (a. anitratus) can cause nosocomially and community acquired pneumonia. source identification of the organism is often difficult. an outbreak of respiratory infection and colonization with a. anitratus affecting 93 ventilated patients in all six of a hospital's intensive care units (icus) over 10 months is described.19883189366
in-vitro and in-vivo activities of t-3262, a new pyridone carboxylic acid.t-3262[p-toluenesulfonic acid salt of dl-7-(3-amino-1-pyrrolidinyl)-1-(2,4-difluorophenyl)-6-fluoro-1, 4-dihydro-4-oxo-1, 8-naphthyridine-3-carboxylic acid monohydrate] is a new pyridone carboxylic acid with a broad spectrum of antibacterial activity against gram-positive and gram-negative bacteria. the activity of t-3262 against most enterobacteriaceae was comparable with that of ciprofloxacin except proteus spp. and providencia rettgeri and exceeded that of ofloxacin and norfloxacin. the activ ...19883182417
synergistic interactions of ciprofloxacin and extended spectrum beta-lactams or aminoglycosides against acinetobacter calcoaceticus ss. anitratus.the susceptibility of 54 clinical isolates of acinetobacter calcoaceticus ss. anitratus to 16 antimicrobial agents was determined in vitro with inoculum of 10(4) and 10(6) cfu by a standard agar dilution method. the most active agents were imipenem, sch 34343, ciprofloxacin, difloxacin (a-56619), and a-56620. only imipenem and abbott quinolones (a-56619 and a-56620) remained active when tested with the heavier inoculum. except for ticarcillin and ceftazidime, which showed only moderate activity, ...19883180706
cytochrome b-562 from acinetobacter calcoaceticus l.m.d. 79.41. its characteristics and role as electron acceptor for quinoprotein glucose dehydrogenase.a soluble cytochrome b was purified from acinetobacter calcoaceticus l.m.d. 79.41. on the basis of the alpha-band maximum of a reduced preparation, measured at 25 degrees c, it is designated as cytochrome b-562. this cytochrome is a basic monomeric protein (pi 10.2; mr 18,000), containing one protohaem group per molecule. the reduced form, at 25 degrees c, showed absorption bands at 428, 532 and 562 nm. at 77 k the alpha-band shifted to 560 nm (with a shoulder at 558 nm). the reduced cytochrome ...19883178744
[susceptibility of gram-negative bacterial isolates to six beta-lactam and two aminoglycoside antibiotics at our university hospital].susceptibilities of gram-negative bacterial strains isolated at the university hospital in 1987 to six beta-lactam antibiotics (azthreonam cefmetazole, ceftizoxime, latamoxef, aminobenzyl-penicillin and piperacillin) and two aminoglycoside antibiotics (gentamicin and amikacin) were examined by agar dilution and the agar diffusion method. results obtained from both methods correlated well in most of the strains. most of the strains belonging to escherichia coli, klebsiella oxytoca, klebsiella pne ...19883175388
dna sequence of the acinetobacter calcoaceticus catechol 1,2-dioxygenase i structural gene cata: evidence for evolutionary divergence of intradiol dioxygenases by acquisition of dna sequence repetitions.the dna sequence of a 1.6-kilobase-pair sali-kpni acinetobacter calcoaceticus restriction fragment carrying cata, the structural gene for catechol 1,2-dioxygenase i, was determined. the 933-nucleotide gene encodes a protein product with a deduced molecular weight of 34,351. the similarly sized pseudomonas clca gene encodes catechol 1,2-dioxygenase ii, an enzyme with relatively broad substrate specificity and relatively low catalytic efficiency. comparison of the cata and clca sequences demonstra ...19883170486
[susceptibilities of clinical isolates to antibacterial agents. focusing mainly on ofloxacin (first report). reported by the research group for testing ofloxacin susceptibility of clinical isolates].susceptibility tests were carried out on a variety of clinically isolated pathogens using the susceptibility disc method at 197 hospitals in japan between may, 1985 through march, 1986. these tests were organized by the research group for testing ofloxacin susceptibility on clinical isolates, and the results were statistically analyzed. this paper describes a comparison of susceptibilities of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, st ...19883163383
[comparative in vitro study of the activity of 5 quinolones against acinetobacter calcoaceticus].the authors compared the in vitro activity of ofloxacin, pefloxacin, ciprofloxacin, norfloxacin and nalidixic acid against 143 clinical isolates of acinetobacter calcoaceticus. minimal inhibitory concentrations (mics) were determined by the agar dilution method. minimal bactericidal concentrations (mbcs) were measured using microplates. ciprofloxacin, ofloxacin and pefloxacin were substantially active in vitro. mics 50 were respectively 0.72, 0.69 and 1.05 micrograms/ml. geometric means were 0.9 ...19853162142
comparative in-vitro activity of ciprofloxacin against non-fermenters.the in-vitro activity of ciprofloxacin, a quinolone-carboxylic acid derivative, was compared with those of carbenicillin, azlocillin, cefsulodin, ceftazidime, tobramycin and amikacin against 187 non-fermenters. only one of the 131 strains of pseudomonas spp. was not inhibited by 1 mg/l of ciprofloxacin, while these isolates appeared highly resistant to carbenicillin, azlocillin and cefsulodin. ciprofloxacin was also the best agent against flavobacterium, alcaligenes faecalis and acinetobacter ca ...19853159710
comparative activity in vitro of ticarcillin and ticarcillin plus clavulanic acid against enterobacteriaceae, pseudomonas aeruginosa and acinetobacter calcoaceticus. 19873151358
leukopenic and lethal effects of slime from acinetobacter calcoaceticus. 19883150412
[serological typing of acinetobacter calcoaceticus subspecies anitratus strains by soft-agar technique]. 19883146608
[in vitro antibacterial activity of cefpirome in combination with 4 aminoglycosides and 2 fluoroquinolones].combinations of cefpirome with aminoglycosides (gentamicin, tobramycin, netilmicin, amikacin) and of cefpirome with fluorinated quinolones (pefloxacin, ofloxacin) were tested against 20 enterobacteriaceae, 6 pseudomonas aeruginosa, 6 acinetobacter calcoaceticus, 6 group d streptococci and 6 staphylococcus aureus strains, susceptible, intermediate or resistant with a low level to these different compounds. activity of combinations was evaluated using a broth microdilution checkerboard method and ...19883141888
endemic acinetobacter anitratus in a surgical intensive care unit: mechanical ventilators as reservoir.colonization and infection with an endemic multiresistant strain of acinetobacter calcoaceticus variety anitratus had been observed in the surgical intensive care unit of a university hospital since 1982. an outbreak of infection with this endemic, multiresistant acinetobacter anitratus strain occurred between january and september, 1984. after initial attempts at identification of environmental reservoirs had been unsuccessful, risk factors for the acquisition of acinetobacter anitratus were in ...19883141154
in vitro studies of fleroxacin (ro 23-6240), a new trifluorinated quinolone derivative.the in vitro activity of fleroxacin (ro 23-6240) against 441 bacterial isolates was compared with those of ciprofloxacin, ofloxacin, amoxycillin, cefadroxil, cefuroxime and tobramycin. an agar dilution method was used for the determination of minimal inhibitory concentrations (mics). ciprofloxacin showed the highest activity against the enterobacteriaceae, 95% of the isolates were inhibited by 0.06 mg/l, but fleroxacin and ofloxacin were also highly active (mic 90% = 0.5 and 0.25 mg/l, respectiv ...19883141116
[effect of the slime from acinetobacter calcoaceticus on the function of phagocytic cells]. 19873127500
susceptibility of 310 nonfermentative gram-negative bacteria to aztreonam, carumonam, ciprofloxacin, ofloxacin and fleroxacin.the susceptibility of 310 mainly clinically isolated gram-negative nonfermenters to aztreonam, carumonam, ciprofloxacin, ofloxacin and fleroxacin was determined by agar dilution. all 3 quinolones were very active, with mic90 ranges (micrograms/ml) against all organisms ranging between 0.25 and 8 for ciprofloxacin, and 0.25 and 16.0 for ofloxacin and fleroxacin, respectively. aztreonam and carumonam showed less activity than the quinolones on a weight-for-weight basis, with mic90 values ranging f ...19883127127
serum bactericidal activity and postantibiotic effect in serum of patients with urinary tract infection receiving high-dose amikacin.ten patients received a 30-min infusion of amikacin (30 mg/kg) on day 1 and 15 mg/kg on day 2. mean serum creatinine was 1.1 +/- 0.3 (standard deviation) mg/dl before and 1.0 +/- 0.3 mg/dl 3 days after the second infusion. mean serum amikacin concentrations before, at the end of infusion, and 1, 6, 12, and 24 h after 30 and 15 mg/kg were 0, 157, 79, 31, 16, 5, 5, 85, 51, 19, 12, and 5 mg/liter, respectively. five strains each of staphylococcus aureus, staphylococcus epidermidis susceptible and r ...19873116918
[epidemiologic studies of the microbial colonization of severely burned patients].bacteria isolated between 1/1/1983 and 12/31/1984 from the burns of 122 patients in a burns unit at the ruhr-universität bochum were studied. grampositive bacteria were predominant in colonizing the burn wounds (62.5% of all strains isolated). in the patients with more than 40% of total body surface area (tbsa) burn, isolation of staph aureus was most frequent. the exogenous colonization rate with staph. aureus was 86%. coagulase negative staphylococci were identified in 89.6% of all patients (7 ...19873116791
adhesion of bacteria from mixed cell suspension to solid surfaces.the attachment of four species of bacteria to solid surfaces was investigated to determine whether the attachment of one species of bacterium could be influenced by the presence of other attaching or attached species. three types of experiment were done: attachment of bacteria from suspensions containing two species (termed "simultaneous attachment") was compared to attachment of each species in pure culture, the attachment of one species of bacterium to surfaces already colonized by a second sp ...19873115220
combined resistance to quinolones and beta-lactams after in vitro transfer on single drugs.single and combined resistance to quinolones and beta-lactams was determined after serial transfers of 18 selected strains of pseudomonas aeruginosa (5), acinetobacter calcoaceticus (3) and beta-lactamase producing enterobacterial strains (10), in broth dilutions of 4 quinolones and 8 beta-lactams. two definitions for resistance were used: (i) 8-fold mic increase and stability of acquired resistance after five transfers in drug-free broth; and (ii) 8-fold mic increase over the breakpoint level. ...19873109816
emergence of resistant bacterial strains during treatment of infections in the respiratory tract.in order to investigate the frequency of the emergence of resistance during treatment, 1,403 episodes of lower respiratory infection were studied in a general hospital with three departments of chest medicine in a period of four years. in 650 episodes the pathogen was isolated and in 82 of those failure of therapy was accompanied by emergence of resistance to the agent used. factors associated with this phenomenon were: intensive care, tracheostomy, involvement of pseudomonas aeruginosa, enterob ...19863103208
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