Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| remediation of phenol-contaminated soil by a bacterial consortium and acinetobacter calcoaceticus isolated from an industrial wastewater treatment plant. | time-course performance of a phenol-degrading indigenous bacterial consortium, and of acinetobacter calcoaceticus var. anitratus, isolated from an industrial coal wastewater treatment plant was evaluated. this bacterial consortium was able to survive in the presence of phenol concentrations as high as 1200mgl(-1) and the consortium was more fast in degrading phenol than a pure culture of the a. calcoaceticus strain. in a batch system, 86% of phenol biodegradation occurred in around 30h at ph 6.0 ... | 2009 | 18774223 |
| genes involved in the benzoate catabolic pathway in acinetobacter calcoaceticus phea-2. | a putative benm gene encoding a lysr-type regulator located upstream from the bena gene was found in acinetobacter calcoaceticus phea-2. disruption of benm or bena destroyed the ability of phea-2 to utilize benzoate. the benm mutant was used to construct a genomic library for isolation of the complete gene cluster responsible for benzoate degradation. sequence analysis showed that the cluster has three putative operons: benm, benabcde, and benkp. unlike many well-characterized benzoate-degrading ... | 2008 | 18781356 |
| distribution of the multidrug efflux pump genes, adeabc, adede and adeijk, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of acinetobacter baumannii-acinetobacter calcoaceticus complex. | of 112 non-repetitive clinical isolates of acinetobacter baumannii-acinetobacter calcoaceticus complex, 80% were resistant to a variety of structurally unrelated antimicrobials although all isolates were susceptible to minocycline and polymyxin. resistance to carbapenems occurred in 8% of the isolates. the presence of adesr-adeabc, adede and adeijk drug efflux system genes and class 1 integron genes (integrase gene int1) was assessed by polymerase chain reaction (pcr) in relation to the suscepti ... | 2009 | 18790612 |
| prokaryote-eukaryote interactions identified by using caenorhabditis elegans. | prokaryote-eukaryote interactions are ubiquitous and have important medical and environmental significance. despite this, a paucity of data exists on the mechanisms and pathogenic consequences of bacterial-fungal encounters within a living host. we used the nematode caenorhabditis elegans as a substitute host to study the interactions between two ecologically related and clinically troublesome pathogens, the prokaryote, acinetobacter baumannii, and the eukaryote, candida albicans. after co-infec ... | 2008 | 18794525 |
| snake cathelicidin from bungarus fasciatus is a potent peptide antibiotics. | cathelicidins are a family of antimicrobial peptides acting as multifunctional effector molecules of innate immunity, which are firstly found in mammalians. recently, several cathelicidins have also been found from chickens and fishes. no cathelicidins from other non-mammalian vertebrates have been reported. | 2008 | 18795096 |
| non-susceptibility trends among pseudomonas aeruginosa and other non-fermentative gram-negative bacteria from bacteraemias in the uk and ireland, 2001-06. | pseudomonas and acinetobacter spp. are important opportunists, notorious for resistance. pseudomonas spp. are collected in the british society for antimicrobial chemotherapy (bsac) bacteraemia surveillance, with acinetobacter spp. and stenotrophomonas maltophilia well represented in the 'other gram-negatives' group. | 2008 | 18819980 |
| identification of bacterial and fungal components in tobacco and tobacco smoke. | abstract: the microbiological composition of tobacco products was studied using culture and chemical analysis (of tobacco leaves) or chemical analysis only (tobacco and tobacco smoke). the chemical analyses utilized gas chromatography-tandem mass spectrometry for determining 3-hydroxy fatty acids, muramic acid, and ergosterol as markers of respectively lipopolysaccharide (lps), peptidoglycan, and fungal biomass. mesophilic bacteria dominated in both fresh and cured tobacco leaves; a range of add ... | 2008 | 18822161 |
| iterative reconstruction of a global metabolic model of acinetobacter baylyi adp1 using high-throughput growth phenotype and gene essentiality data. | genome-scale metabolic models are powerful tools to study global properties of metabolic networks. they provide a way to integrate various types of biological information in a single framework, providing a structured representation of available knowledge on the metabolism of the respective species. | 2008 | 18840283 |
| gibberellin production and phosphate solubilization by newly isolated strain of acinetobacter calcoaceticus and its effect on plant growth. | plant growth-promoting rhizobacteria with gibberellins (ga)-producing potential were isolated from soil and screened for plant growth promotion. a new strain, acinetobacter calcoaceticus se370, produced extracellular ga and also had phosphate solubilising potential. it produced 10 different gibberellins, including the bioactive ga(1), ga(3) and ga(4) which were at, respectively, 0.45, 6.2 and 2.8 ng/100 ml. the isolate solubilised tricalcium phosphate and lowered ph of the medium during the proc ... | 2009 | 18931973 |
| biodegradation of swainsonine by acinetobacter calcoaceticus strain ylzz-1 and its isolation and identification. | eight swainsonine (sw)-degrading bacteria were isolated from the soil where locoweed was buried for 6 months and one of the strains (ylzz-1) was selected for further study. based on morphology, physiologic tests, 16s rrna gene sequence, and phylogenetic characteristics, the strain showed the greatest similarity to members of the order acinetobacters and within the order to members of the acinetobacter calcoaceticus group. the ability of the strain for degrading sw, as sole carbon source, was inv ... | 2009 | 18931977 |
| comparative analysis of viperidae venoms antibacterial profile: a short communication for proteomics. | bacterial infections involving multidrug-resistant strains are one of the ten leading causes of death and an important health problem in need for new antibacterial sources and agents. herein, we tested and compared four snake venoms (agkistrodon rhodostoma, bothrops jararaca, b. atrox and lachesis muta) against 10 gram-positive and gram-negative drug-resistant clinical bacteria strains to identify them as new sources of potential antibacterial molecules. our data revealed that, as efficient as s ... | 2008 | 18955360 |
| the rna polymerase "switch region" is a target for inhibitors. | the alpha-pyrone antibiotic myxopyronin (myx) inhibits bacterial rna polymerase (rnap). here, through a combination of genetic, biochemical, and structural approaches, we show that myx interacts with the rnap "switch region"--the hinge that mediates opening and closing of the rnap active center cleft--to prevent interaction of rnap with promoter dna. we define the contacts between myx and rnap and the effects of myx on rnap conformation and propose that myx functions by interfering with opening ... | 2008 | 18957204 |
| establishment of in vitro susceptibility testing methodologies and comparative activities of piperacillin in combination with the penem {beta}-lactamase inhibitor bli-489. | the novel bicyclic penem inhibitor bli-489 has demonstrated activity as an inhibitor of class a, c, and d beta-lactamases. to determine the combination of piperacillin and bli-489 to be used in susceptibility testing that would most accurately identify susceptible and resistant isolates, a predictor panel of beta-lactamase-producing bacteria was utilized to determine the reliability of the combination of piperacillin-bli-489 at a constant inhibitor concentration of 2 or 4 microg/ml and at ratios ... | 2008 | 19001109 |
| establishment of in vitro susceptibility testing methodologies and comparative activities of piperacillin in combination with the penem {beta}-lactamase inhibitor bli-489. | the novel bicyclic penem inhibitor bli-489 has demonstrated activity as an inhibitor of class a, c, and d beta-lactamases. to determine the combination of piperacillin and bli-489 to be used in susceptibility testing that would most accurately identify susceptible and resistant isolates, a predictor panel of beta-lactamase-producing bacteria was utilized to determine the reliability of the combination of piperacillin-bli-489 at a constant inhibitor concentration of 2 or 4 microg/ml and at ratios ... | 2008 | 19001109 |
| a constructed alkaline consortium and its dynamics in treating alkaline black liquor with very high pollution load. | paper pulp wastewater resulting from alkaline extraction of wheat straw, known as black liquor, is very difficult to be treated and causes serious environmental problems due to its high ph value and chemical oxygen demand (cod) pollution load. lignin, semicellulose and cellulose are the main contributors to the high cod values in black liquor. very few microorganisms can survive in such harsh environments of the alkaline wheat straw black liquor. a naturally developed microbial community was fou ... | 2008 | 19020664 |
| genetic variability among ampc genes from acinetobacter genomic species 3. | as a part of a nationwide study in spain, 15 clinical isolates of acinetobacter genomic species 3 (ag3) were analyzed. the main objective of the study was to characterize the ampc genes from these isolates and to determine their involvement in beta-lactam resistance in ag3. the 15 ag3 isolates showed different profiles of resistance to ampicillin (range of mics, 12 to >256 microg/ml). nucleotide sequencing of the 15 ampc genes yielded 12 new ampc enzymes (adc-12 to adc-23). the 12 ag3 enzymes sh ... | 2009 | 19029333 |
| genetic variability among ampc genes from acinetobacter genomic species 3. | as a part of a nationwide study in spain, 15 clinical isolates of acinetobacter genomic species 3 (ag3) were analyzed. the main objective of the study was to characterize the ampc genes from these isolates and to determine their involvement in beta-lactam resistance in ag3. the 15 ag3 isolates showed different profiles of resistance to ampicillin (range of mics, 12 to >256 microg/ml). nucleotide sequencing of the 15 ampc genes yielded 12 new ampc enzymes (adc-12 to adc-23). the 12 ag3 enzymes sh ... | 2009 | 19029333 |
| biosynthesis of storage compounds by rhodococcus jostii rha1 and global identification of genes involved in their metabolism. | members of the genus rhodococcus are frequently found in soil and other natural environments and are highly resistant to stresses common in those environments. the accumulation of storage compounds permits cells to survive and metabolically adapt during fluctuating environmental conditions. the purpose of this study was to perform a genome-wide bioinformatic analysis of key genes encoding metabolism of diverse storage compounds by rhodococcus jostii rha1 and to examine its ability to synthesize ... | 2008 | 19077282 |
| wide dissemination of oxa-type carbapenemases in clinical acinetobacter spp. isolates from south korea. | carbapenem-resistant acinetobacter spp. are being increasingly reported worldwide, including in south korea, where we examined 144 representative isolates collected in a nationwide hospital survey in 2005. metallo-beta-lactamases were detected in only 19.4% of isolates, none of which were acinetobacter baumannii, whereas 74.3% of isolates (mostly a. baumannii) expressed bla(oxa) carbapenemase genes. among the latter, 47 had bla(oxa-23)-like genes and 56 had upregulated bla(oxa-51)-like variants, ... | 2009 | 19091520 |
| surface translocation by legionella pneumophila: a form of sliding motility that is dependent upon type ii protein secretion. | legionella pneumophila exhibits surface translocation when it is grown on a buffered charcoal yeast extract (bcye) containing 0.5 to 1.0% agar. after 7 to 22 days of incubation, spreading legionellae appear in an amorphous, lobed pattern that is most manifest at 25 to 30 degrees c. all nine l. pneumophila strains examined displayed the phenotype. surface translocation was also exhibited by some, but not all, other legionella species examined. l. pneumophila mutants that were lacking flagella and ... | 2009 | 19114479 |
| surface translocation by legionella pneumophila: a form of sliding motility that is dependent upon type ii protein secretion. | legionella pneumophila exhibits surface translocation when it is grown on a buffered charcoal yeast extract (bcye) containing 0.5 to 1.0% agar. after 7 to 22 days of incubation, spreading legionellae appear in an amorphous, lobed pattern that is most manifest at 25 to 30 degrees c. all nine l. pneumophila strains examined displayed the phenotype. surface translocation was also exhibited by some, but not all, other legionella species examined. l. pneumophila mutants that were lacking flagella and ... | 2009 | 19114479 |
| utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of acinetobacter spp. and pseudomonas aeruginosa. | the utility of peptide nucleic acid fluorescence in situ hybridization (pna fish) for the detection of acinetobacter spp. and pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of atcc strains and clinical isolates with select gram-negative rods. after testing 60 clinical isolates, pna fish had a sensitivity and specificity of 100% and 100%, respectively, for acinetobacter spp. and 100% and 95%, respectively, for p. aeruginosa. pna fish was able to detect both pa ... | 2009 | 19116347 |
| utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of acinetobacter spp. and pseudomonas aeruginosa. | the utility of peptide nucleic acid fluorescence in situ hybridization (pna fish) for the detection of acinetobacter spp. and pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of atcc strains and clinical isolates with select gram-negative rods. after testing 60 clinical isolates, pna fish had a sensitivity and specificity of 100% and 100%, respectively, for acinetobacter spp. and 100% and 95%, respectively, for p. aeruginosa. pna fish was able to detect both pa ... | 2009 | 19116347 |
| risk factors for mortality in patients with acinetobacter baumannii bloodstream infection with genotypic species identification. | acinetobacter baumannii is an increasingly common nosocomial infection with a high mortality rate. identification of predictor factors of mortality from a. baumannii infection is important for the implementation of therapeutic management for patients with higher risk. however, many studies have reported data for acinetobacter calcoaceticus-a. baumannii complex, which might lead to an uncertainty of results. in this study, we aimed to identify the predictive factors for mortality of patients infe ... | 2008 | 19122921 |
| temporal appearance of plasmid-mediated quinolone resistance genes. | one hundred fifty aac(6')-ib-positive gram-negative isolates collected between 1981 and 1991 were examined by pcr for the presence of the aac(6')-ib-cr variant and other plasmid-mediated quinolone resistance (pmqr) genes. none had the aac(6')-ib-cr variant, qnra, qnrs, qnrc, or qepa, but two strains collected in 1988 had qnrb alleles, making these the earliest known pmqr genes. | 2009 | 19164145 |
| the effect of bacterial glutathione s-transferase on morpholine degradation. | glutathione s-transferases (gsts) constitute a large family of enzymes that catalyze the addition of glutathione to endogenous, or xenobiotic, often toxic electrophilic compounds. the effect of this enzyme in facilitating polychlorinated biphenyls degradation has been studied previously. here the effects of induced cell-free extracts of acinetobacter calcoaceticus and pseudomonas aeruginosa (grown on hexadecane), and e. coli bl21 (induced with pgex-2t plasmid on isothiopropylgalactoside) were re ... | 2009 | 19194977 |
| decontamination of laundry at low temperature with cuwb50, a novel copper-based biocidal compound. | traditional laundry decontamination relies on thermal disinfection that degrades textiles. we investigated the ability of a novel copper-based biocidal compound, cuwb50, to assist in the decontamination of swatches purposely contaminated with staphylococcus aureus and acinetobacter during "real-life" low-temperature machine washing with and without 2 commercial detergents. | 2009 | 19216003 |
| treatment of war wounds: a historical review. | the treatment of war wounds is an ancient art, constantly refined to reflect improvements in weapons technology, transportation, antiseptic practices, and surgical techniques. throughout most of the history of warfare, more soldiers died from disease than combat wounds, and misconceptions regarding the best timing and mode of treatment for injuries often resulted in more harm than good. since the 19th century, mortality from war wounds steadily decreased as surgeons on all sides of conflicts dev ... | 2009 | 19219516 |
| evolution of enzymatic activities in the enolase superfamily: stereochemically distinct mechanisms in two families of cis,cis-muconate lactonizing enzymes. | the mechanistically diverse enolase superfamily is a paradigm for elucidating nature's strategies for divergent evolution of enzyme function. each of the different reactions catalyzed by members of the superfamily is initiated by abstraction of the alpha-proton of a carboxylate substrate that is coordinated to an essential mg(2+). the muconate lactonizing enzyme (mle) from pseudomonas putida, a member of a family that catalyzes the syn-cycloisomerization of cis,cis-muconate to (4s)-muconolactone ... | 2009 | 19220063 |
| microbiology of the external ear canal in six african elephants (loxodonta africana). | samples collected from both external ear canals of six adult female african elephants (loxodonta africana) were cultured for fungi, yeasts and aerobic and anaerobic bacteria. all the samples produced heavy growths of several aerobic bacteria, but anaerobic bacteria were rare and no fungi or yeasts were isolated. the most common bacterium isolated was staphylococcus epidermidis, which was cultured from 11 of the 12 ears. acinetobacter calcoaceticus lwoffi, alpha-haemolytic streptococcus and coryn ... | 2009 | 19234325 |
| dna as an adhesin: bacillus cereus requires extracellular dna to form biofilms. | the soil saprophyte bacillus cereus forms biofilms at solid-liquid interfaces. the composition of the extracellular polymeric matrix is not known, but biofilms of other bacteria are encased in polysaccharides, protein, and also extracellular dna (edna). a tn917 screen for strains impaired in biofilm formation at a solid-liquid interface yielded several mutants. three mutants deficient in the purine biosynthesis genes pura, purc, and purl were biofilm impaired, but they grew planktonically like t ... | 2009 | 19251901 |
| plant and animal wastes composting: effects of the n source on process performance. | the aim of this work was to evaluate the impact of different n-rich animal wastes on the composting of ligno-cellulosic wastes by a range of classical and novel methods, with particular emphasis on microbial community composition. two composting mixtures were prepared by adding to a mixture of cotton carding wastes and wheat straw: (i) meat and bone meal and (ii) blood meal and horn and hoof meal. composts were analyzed using physico-chemical and biochemical properties, as well as nucleic acid m ... | 2009 | 19264479 |
| genomic analysis of "elusimicrobium minutum," the first cultivated representative of the phylum "elusimicrobia" (formerly termite group 1). | organisms of the candidate phylum termite group 1 (tg1) are regularly encountered in termite hindguts but are present also in many other habitats. here, we report the complete genome sequence (1.64 mbp) of "elusimicrobium minutum" strain pei191(t), the first cultured representative of the tg1 phylum. we reconstructed the metabolism of this strictly anaerobic bacterium isolated from a beetle larva gut, and we discuss the findings in light of physiological data. e. minutum has all genes required f ... | 2009 | 19270133 |
| tetracycline susceptibility testing and resistance genes in isolates of acinetobacter baumannii-acinetobacter calcoaceticus complex from a u.s. military hospital. | infections with multidrug-resistant acinetobacter baumannii-acinetobacter calcoaceticus complex bacteria complicate the care of u.s. military personnel and civilians worldwide. one hundred thirty-three isolates from 89 patients at our facility during 2006 and 2007 were tested by disk diffusion, etest, and broth microdilution for susceptibility to tetracycline, doxycycline, minocycline, and tigecycline. minocycline was the most active in vitro, with 90% of the isolates tested susceptible. suscept ... | 2009 | 19307365 |
| management of meningitis due to antibiotic-resistant acinetobacter species. | acinetobacter meningitis is becoming an increasingly common clinical entity, especially in the postneurosurgical setting, with mortality from this infection exceeding 15%. infectious diseases society of america guidelines for therapy of postneurosurgical meningitis recommend either ceftazidime or cefepime as empirical coverage against gram-negative pathogens. however, assessment of the pharmacodynamics of these cephalosporins in cerebrospinal fluid suggests that recommended doses will achieve ph ... | 2009 | 19324297 |
| regulation of acinetobacter baumannii biofilm formation. | acinetobacter baumannii is a gram-negative opportunistic nosocomial pathogen. this microorganism survives in hospital environments despite unfavorable conditions such as desiccation, nutrient starvation and antimicrobial treatments. it is hypothesized that its ability to persist in these environments, as well as its virulence, is a result of its capacity to form biofilms. a. baumannii forms biofilms on abiotic surfaces such as polystyrene and glass as well as biotic surfaces such as epithelial c ... | 2009 | 19327114 |
| identification of acinetobacter genomic species 13tu by sequence analysis of the 16s-23s rrna gene spacer region. | 2009 | 19339482 | |
| coaggregation by the freshwater bacterium sphingomonas natatoria alters dual-species biofilm formation. | coaggregation is hypothesized to enhance freshwater biofilm development. to investigate this hypothesis, the ability of the coaggregating bacterium sphingomonas natatoria to form single- and dual-species biofilms was studied and compared to that of a naturally occurring spontaneous coaggregation-deficient variant. attachment assays using metabolically inactive cells were performed using epifluorescence and confocal laser scanning microscopy. under static and flowing conditions, coaggregating s. ... | 2009 | 19376917 |
| genetic and biochemical characterization of the first extended-spectrum carb-type beta-lactamase, rtg-4, from acinetobacter baumannii. | acinetobacter baumannii isolate kar was uncommonly more resistant to cefepime and cefpirome than to ceftazidime and cefotaxime. cloning and expression of the beta-lactamase gene content of this isolate into escherichia coli top10 identified ss-lactamase rtg-4 (or carb-10), which corresponds to the first reported extended-spectrum carb-type enzyme. rtg-4 is a plasmid-encoded ambler class a beta-lactamase whose sequence differs by 4 amino acid substitutions from the narrow-spectrum beta-lactamase ... | 2009 | 19380596 |
| validation of partial rpob gene sequence analysis for the identification of clinically important and emerging acinetobacter species. | bacteria belonging to the genus acinetobacter are ubiquitous in soil and water. only a few species, including acinetobacter baumannii, and the unnamed acinetobacter genomic species (gen. sp.) 3 and 13tu, which together with the soil organism acinetobacter calcoaceticus are combined in the a. calcoaceticus-a. baumannii (acb) complex, have been recognized as important nosocomial infectious agents. the ecology, epidemiology and pathology of most species are not yet well established. lack of practic ... | 2009 | 19389786 |
| reverse transcription of 16s rrna to monitor ribosome-synthesizing bacterial populations in the environment. | identification and quantification of phylogenetically defined bacterial populations in the environment are often performed using molecular tools targeting 16s rrna. fluorescence in situ hybridization has been used to monitor the expression and processing of rrna by targeting the 3' tail of precursor 16s rrna. to expand this approach, we employed reverse transcription of total rna using primer s-d-bact-0338-a-a-18. length heterogeneity detected by slab gel analysis, denaturing high-performance li ... | 2009 | 19395563 |
| productivity of cyclohexanone oxidation of the recombinant corynebacterium glutamicum expressing chnb of acinetobacter calcoaceticus. | the biocatalytic efficiency of recombinant corynebacterium glutamicum expressing the chnb gene encoding cyclohexanone monooxygenase (chmo) of acinetobacter calcoaceticus ncimb 9871 was investigated. optimization of an expression system and induction conditions enabled the recombinant biocatalyst to produce chmo to a specific activity of ca. 0.5 u mg(-1) protein. tight control of feeding of an energy source (i.e., glucose) and dissolved oxygen tension during fed-batch culture-based biotransformat ... | 2009 | 19397940 |
| detection and characterization of class 1 integrons among carbapenem-resistant isolates of acinetobacter spp. in malaysia. | to detect and characterize class 1 integrons among carbapenem-resistant strains of acinetobacter spp. at university malaya medical centre (ummc), kuala lumpur, malaysia. | 2009 | 19424559 |
| rapid purification and characterization of a novel heparin degrading enzyme from acinetobacter calcoaceticus. | an intracellularly produced constitutive heparinase was isolated from the periplasmic space of acinetobacter calcoaceticus by freeze fracturing and purified 51.2-fold by ion exchange and gel filtration chromatography. specific activity of the purified enzyme was found to be 41 iu/mug protein with a 120000da molecular mass. the enzyme activity was maximum at 35 degrees c in the presence of 250mm nacl at ph 7.5. the enzyme activity was inhibited in the presence of ba(2+), hg(2+), cd(2+), iaa and d ... | 2009 | 19426846 |
| biosurfactants from acinetobacter calcoaceticus bu03 enhance the solubility and biodegradation of phenanthrene. | a thermophilic bacterial strain, acinetobacter calcoaceticus bu03, with a biosurfactant-producing capability, was isolated from petroleum-contaminated soil with an improved procedure which employed the solubilization of polycyclic aromatic hydrocarbons (pahs), i.e. naphthalene in agar plate, as a selection criterion. crude biosurfactant was recovered from the culture of bu03 by extraction with n-hexane, and its properties were investigated. biosurfactants from a. calcoaceticus bu03 constitute a ... | 2009 | 19438062 |
| long-term control of hospital-wide, endemic multidrug-resistant acinetobacter baumannii through a comprehensive "bundle" approach. | acinetobacter baumannii (ab) is emerging as a multidrug-resistant (mdr) nosocomial pathogen of considerable clinical importance. data on the efficacy of infection control measures in endemic situations are lacking. here, we investigated the impact of a long-term multifaceted "bundle" approach in controlling endemic mdr ab in a 950-bed tertiary care center. | 2009 | 19457584 |
| rapid identification of biothreat and other clinically relevant bacterial species by use of universal pcr coupled with high-resolution melting analysis. | a rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize pcr products. unique melt profiles generated from multiple hypervariable regions of the 16s rrna gene for 100 clinically relevant bacterial pathogens, including category a and b biothreat agents and their surrogates, allowed highly specific species identification. | 2009 | 19458181 |
| in vitro activity of tigecycline in combination with various antimicrobials against multidrug resistant acinetobacter baumannii. | infections sustained by multidrug-resistant (mdr) and pan-resistant acinetobacter baumannii have become a challenging problem in intensive care units. tigecycline provided new hope for the treatment of mdr a. baumannii infections, but isolates showing reduced susceptibility have emerged in many countries, further limiting the therapeutic options. empirical combination therapy has become a common practice to treat patients infected with mdr a. baumannii, in spite of the limited microbiological an ... | 2009 | 19460166 |
| acinetobacter strains ih9 and oci1, two rhizospheric phosphate solubilizing isolates able to promote plant growth, constitute a new genomovar of acinetobacter calcoaceticus. | during a screening of phosphate solubilizing bacteria (psb) in agricultural soils, two strains, ih9 and oci1, were isolated from the rhizosphere of grasses in spain, and they showed a high ability to solubilize phosphate in vitro. inoculation experiments in chickpea and barley were conducted with both strains and the results demonstrated their ability to promote plant growth. the 16s rrna gene sequences of these strains were nearly identical to each other and to those of acinetobacter calcoaceti ... | 2009 | 19467815 |
| the acinetobacter baumannii 19606 ompa protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. | the ability of acinetobacter baumannii to adhere to and persist on surfaces as biofilms could be central to its pathogenicity. the production of pili and a biofilm-associated protein and the expression of antibiotic resistance are needed for robust biofilm formation on abiotic and biotic surfaces. this multistep process also depends on the expression of transcriptional regulatory functions, some of which could sense nutrients available to cells. this report extends previous observations by showi ... | 2009 | 19470746 |
| isolation and characterization of rhamnolipid-producing bacterial strains from a biodiesel facility. | novel strains of rhamnolipid-producing bacteria were isolated from soils at a biodiesel facility on the basis of their ability to grow on glycerol as a sole carbon source. strains were identified as acinetobacter calcoaceticus, enterobacter asburiae, enterobacter hormaechei, pantoea stewartii, and pseudomonas aeruginosa. the strains of the former five species were found to produce rhamnolipids in quantities the same as, or similar to, coisolated strains of p. aeruginosa. measurements of surface ... | 2009 | 19473254 |
| the genome sequence of geobacter metallireducens: features of metabolism, physiology and regulation common and dissimilar to geobacter sulfurreducens. | the genome sequence of geobacter metallireducens is the second to be completed from the metal-respiring genus geobacter, and is compared in this report to that of geobacter sulfurreducens in order to understand their metabolic, physiological and regulatory similarities and differences. | 2009 | 19473543 |
| synergistic interaction between silver nanoparticles and membrane-permeabilizing antimicrobial peptides. | silver nanoparticles, as well as antimicrobial peptides (amps), can be used to fight infectious diseases. since amps are known to permeabilize bacterial membranes and might therefore help silver nanoparticles to access internal target sites, we investigated their combined activities and showed synergistic effects between polymyxin b and silver nanoparticles for gram-negative bacteria. | 2009 | 19528287 |
| minor trauma triggering cervicofacial necrotizing fasciitis from odontogenic abscess. | necrotizing fasciitis (nf) of the face and neck is a very rare complication of dental infection. otolaryngologists and dentists should be familiar with this condition because of its similarity to odontogenic deep neck space infection in the initial stages, its rapid spread, and its life-threatening potential. trauma has been reported to be an important predisposing factor for nf of the face. in this paper, we describe the presentation and treatment of a 62-year-old man who developed nf of the fa ... | 2008 | 19561990 |
| clinical features and molecular epidemiology of multidrug-resistant acinetobacter calcoaceticus-a baumannii complex in a regional teaching hospital in taiwan. | we conducted a case-controlled study in a regional teaching hospital in taiwan to investigate the clinical features and molecular epidemiology of multidrug-resistant acinetobacter calcoaceticus-a baumannii (mdr acb) complex. case patients had higher mortality than controls did. mdr acb complex acquisition risk factors include longer hospital stays, higher ratio of nasogastric tube and foley catheter use, and more carbapenem use. all available isolates were divided into 36 subtypes by pulsed-fiel ... | 2009 | 19576662 |
| benzoate catabolite repression of the phenol degradation in acinetobacter calcoaceticus phea-2. | acinetobacter calcoaceticus phea-2 exhibited a delayed utilization of phenol in the presence of benzoate. benzoate supplementation completely inhibited phenol degradation in a benzoate 1,2-dioxygenase knockout mutant. the mphr encoding the transcriptional activator and mphn encoding the largest subunit of multi-component phenol hydroxylase in the bena mutant were significantly downregulated (about 7- and 70-fold) on the basis of mrna levels when benzoate was added to the medium. the co-transform ... | 2009 | 19597885 |
| persistent wolbachia and cultivable bacteria infection in the reproductive and somatic tissues of the mosquito vector aedes albopictus. | commensal and symbiotic microbes have a considerable impact on the behavior of many arthropod hosts, including hematophagous species that transmit pathogens causing infectious diseases to human and animals. little is known about the bacteria associated with mosquitoes other than the vectorized pathogens. this study investigated wolbachia and cultivable bacteria that persist through generations in ae. albopictus organs known to host transmitted arboviruses, such as dengue and chikungunya. | 2009 | 19633721 |
| species composition of bacterial communities influences attraction of mosquitoes to experimental plant infusions. | in the container habitats of immature mosquitoes, catabolism of plant matter and other organic detritus by microbial organisms produces metabolites that mediate the oviposition behavior of aedes aegypti and aedes albopictus. public health agencies commonly use oviposition traps containing plant infusions for monitoring populations of these mosquito species, which are global vectors of dengue viruses. in laboratory experiments, gravid females exhibited significantly diminished responses to experi ... | 2009 | 19641948 |
| species composition of bacterial communities influences attraction of mosquitoes to experimental plant infusions. | in the container habitats of immature mosquitoes, catabolism of plant matter and other organic detritus by microbial organisms produces metabolites that mediate the oviposition behavior of aedes aegypti and aedes albopictus. public health agencies commonly use oviposition traps containing plant infusions for monitoring populations of these mosquito species, which are global vectors of dengue viruses. in laboratory experiments, gravid females exhibited significantly diminished responses to experi ... | 2009 | 19641948 |
| rapid detection of vaginal candida species by newly developed immunochromatography. | for the diagnosis of vulvovaginal candidiasis, we developed a simple immunochromatographic method that enables the detection of vaginal candida spp. within about 30 min. overall, the sensitivity, specificity, positive predictive value, and negative predictive value of this method appeared to be 80.3, 99.3, 98.0, and 92.0%, respectively. | 2009 | 19656990 |
| efflux-mediated drug resistance in bacteria: an update. | drug efflux pumps play a key role in drug resistance and also serve other functions in bacteria. there has been a growing list of multidrug and drug-specific efflux pumps characterized from bacteria of human, animal, plant and environmental origins. these pumps are mostly encoded on the chromosome, although they can also be plasmid-encoded. a previous article in this journal provided a comprehensive review regarding efflux-mediated drug resistance in bacteria. in the past 5 years, significant pr ... | 2009 | 19678712 |
| occurrence of oxa-107 and isaba1 in carbapenem-resistant isolates of acinetobacter baumannii from croatia. | carbapenem-resistant isolates of acinetobacter baumannii from intensive care units at split university hospital, split, croatia, were studied. most (100 of 106) had isaba1 inserted upstream of a bla(oxa-107) gene, encoding an unusual oxa-51-type oxacillinase. pulsed-field gel electrophoresis revealed that the isolates formed three clusters belonging to the sequence group 2 (european clone 1) lineage. | 2009 | 19692565 |
| impact of (+/-)-catechin on soil microbial communities. | catechin is a highly studied but controversial allelochemical reported as a component of the root exudates of centaurea maculosa. initial reports of high and consistent exudation rates and soil concentrations have been shown to be highly inaccurate, but the chemical has been found in root exudates at and much less frequently in soil but sporadically at high concentrations. part of the problem of detection and measuring phytotoxicity in natural soils may be due to the confounding effect of soil m ... | 2009 | 19704908 |
| factors influencing survival in patients with multi-drug-resistant acinetobacter bacteraemia. | the incidence of multi-drug-resistant (mdr) acinetobacter strains is increasing and therapeutic options are limited. however, controversy exists regarding the mortality attributable to antimicrobial resistance. the aim of this study was to analyse the clinical features and outcomes of patients with mdr acinetobacter calcoaceticus-acinetobacter baumannii complex (acb complex) bacteraemia and determine the factors influencing survival by using 14-day mortality as the main outcome measure. | 2009 | 19712862 |
| mutations that disrupt either the pqq or the gdh gene of rahnella aquatilis abolish the production of an antibacterial substance and result in reduced biological control of grapevine crown gall. | rahnella aquatilis hx2, a biocontrol agent for grapevine crown gall caused by agrobacterium vitis, produces an antibacterial substance that inhibits the growth of a. vitis in vitro. in this study, we show that mh15 and mh16, two tn5-induced mutants of hx2, have lost their abilities to inhibit a. vitis and have reduced biocontrol activities; they grow in logarithmic phase at a rate similar to that of the wild type and have single tn5 insertions. they are also impaired in producing pyrroloquinolin ... | 2009 | 19734331 |
| pyrroloquinoline quinone biogenesis: demonstration that pqqe from klebsiella pneumoniae is a radical s-adenosyl-l-methionine enzyme. | biogenesis of pyrroloquinoline quinone (pqq) in klebsiella pneumoniae requires the expression of six genes (pqqa-f). one of these genes (pqqe) encodes a 43 kda protein (pqqe) that plays a role in the initial steps in pqq formation [veletrop, j. s., et al. (1995) j. bacteriol. 177, 5088-5098]. pqqe contains two highly conserved cysteine motifs at the n- and c-termini, with the n-terminal motif comprised of a cx(3)cx(2)c consensus sequence that is unique to a family of proteins known as radical s- ... | 2009 | 19746930 |
| development and validation of a real-time pcr for detection of pathogenic leptospira species in clinical materials. | available serological diagnostics do not allow the confirmation of clinically suspected leptospirosis at the early acute phase of illness. several conventional and real-time pcrs for the early diagnosis of leptospirosis have been described but these have been incompletely evaluated. we developed a sybr green-based real-time pcr targeting secy and validated it according to international guidelines. to determine the analytical specificity, dna from 56 leptospira strains belonging to pathogenic, no ... | 2009 | 19763264 |
| prokaryotic phototaxis. | microorganisms have various mechanisms at their disposal to react to (changes in) their ambient light climate (i.e., intensity, color, direction, and degree of polarization). of these, one of the best studied mechanisms is the process of phototaxis. this process can be described as a behavioral migration-response of an organism toward a change in illumination regime. in this chapter we discuss three of these migration responses, based on swimming, swarming, and twitching motility, respectively. ... | 2009 | 19763957 |
| lipid body formation plays a central role in cell fate determination during developmental differentiation of myxococcus xanthus. | cell differentiation is widespread during the development of multicellular organisms, but rarely observed in prokaryotes. one example of prokaryotic differentiation is the gram-negative bacterium myxococcus xanthus. in response to starvation, this gliding bacterium initiates a complex developmental programme that results in the formation of spore-filled fruiting bodies. how the cells metabolically support the necessary complex cellular differentiation from rod-shaped vegetative cells into spheri ... | 2009 | 19788540 |
| production of cell-cell signalling molecules by bacteria isolated from human chronic wounds. | to (i) identify chronic wound bacteria and to test their ability to produce acyl-homoserine-lactones (ahls) and autoinducer-2 (ai-2) cell-cell signalling molecules and (ii) determine whether chronic wound debridement samples might contain these molecules. | 2010 | 19840177 |
| production of cell-cell signalling molecules by bacteria isolated from human chronic wounds. | to (i) identify chronic wound bacteria and to test their ability to produce acyl-homoserine-lactones (ahls) and autoinducer-2 (ai-2) cell-cell signalling molecules and (ii) determine whether chronic wound debridement samples might contain these molecules. | 2010 | 19840177 |
| spectroscopic studies of the oxidation of ferric cyp153a6 by peracids: insights into p450 higher oxidation states. | our previous rapid-scanning stopped-flow studies of the reaction of substrate-free cytochrome p450cam with peracids [t. spolitak, j.h. dawson, d.p. ballou, j. biol. chem. 280 (2005) 20300-20309; j. inorg. biochem. 100 (2006) 2034-2044; j. biol. inorg. chem. 13 (2008) 599-611] spectrally characterized compound i (ferryl iron plus a porphyrin pi-cation radical (fe(iv)o/por(.+))), cpd es, and cpd ii (fe(iv)o/tyr() or fe(iv)o). we now report that reactions of cyp153a6 with peracids yield all these i ... | 2010 | 19879854 |
| spectroscopic studies of the oxidation of ferric cyp153a6 by peracids: insights into p450 higher oxidation states. | our previous rapid-scanning stopped-flow studies of the reaction of substrate-free cytochrome p450cam with peracids [t. spolitak, j.h. dawson, d.p. ballou, j. biol. chem. 280 (2005) 20300-20309; j. inorg. biochem. 100 (2006) 2034-2044; j. biol. inorg. chem. 13 (2008) 599-611] spectrally characterized compound i (ferryl iron plus a porphyrin pi-cation radical (fe(iv)o/por(.+))), cpd es, and cpd ii (fe(iv)o/tyr() or fe(iv)o). we now report that reactions of cyp153a6 with peracids yield all these i ... | 2010 | 19879854 |
| contact precautions for multidrug-resistant organisms: current recommendations and actual practice. | contact precautions are recommended for interactions with patients colonized/infected with multidrug-resistant organisms; however, actual rates of implementation of contact precautions are unknown. | 2010 | 19913329 |
| contact precautions for multidrug-resistant organisms: current recommendations and actual practice. | contact precautions are recommended for interactions with patients colonized/infected with multidrug-resistant organisms; however, actual rates of implementation of contact precautions are unknown. | 2010 | 19913329 |
| pneumatocele formation in adult pulmonary tuberculosis during antituberculous chemotherapy: a case report. | pulmonary pneumatoceles are thin-walled, air-filled cysts that develop within the lung. most often, they occur as a sequela to acute pneumonia, commonly caused by staphylococcus aureus, and are found more frequently in infants and young children. adult tuberculous pulmonary pneumatoceles are seldom reported. | 2009 | 19918388 |
| rapid universal identification of bacterial pathogens from clinical cultures by using a novel sloppy molecular beacon melting temperature signature technique. | a real-time pcr assay with the ability to rapidly identify all pathogenic bacteria would have widespread medical utility. current real-time pcr technologies cannot accomplish this task due to severe limitations in multiplexing ability. to this end, we developed a new assay system which supports very high degrees of multiplexing. we developed a new class of mismatch-tolerant "sloppy" molecular beacons, modified them to provide an extended hybridization range, and developed a multiprobe, multimelt ... | 2009 | 19923485 |
| rapid universal identification of bacterial pathogens from clinical cultures by using a novel sloppy molecular beacon melting temperature signature technique. | a real-time pcr assay with the ability to rapidly identify all pathogenic bacteria would have widespread medical utility. current real-time pcr technologies cannot accomplish this task due to severe limitations in multiplexing ability. to this end, we developed a new assay system which supports very high degrees of multiplexing. we developed a new class of mismatch-tolerant "sloppy" molecular beacons, modified them to provide an extended hybridization range, and developed a multiprobe, multimelt ... | 2009 | 19923485 |
| extended spectrum beta-lactamases among gram-negative bacterial isolates from clinical specimens in three major hospitals in northern jordan. | background and objectives. extended spectrum beta-lactamase (esbl) production is increasing all over the world, and organisms other than e. coli and k. pneumoniae are acquiring this character. esbl production is detectable by automation, e-test, double disk diffusion (ddd), and pcr. this study aimed to determine the prevalence of esbl production among clinical isolates of gram-negative rods, and to evaluate the effectiveness of augmentation of clavunate with cefotaxime, ceftazoxime, aztreonam, c ... | 2009 | 19936109 |
| a beta-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in cupriavidus necator. | the tcprxabcyd operon of cupriavidus necator jmp134 is involved in the degradation of 2,4,6-trichlorophenol (tcp). all of the gene products except tcpy have assigned functions in tcp metabolism. sequence comparison identified tcpy as a member of cog4313, a group of hypothetical proteins. tcpy has a signal peptide, indicating it is a membrane or secreted protein. secondary structure and topology analysis indicated tcpy as a beta-barrel outer membrane protein, similar to the escherichia coli outer ... | 2010 | 19937267 |
| a beta-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in cupriavidus necator. | the tcprxabcyd operon of cupriavidus necator jmp134 is involved in the degradation of 2,4,6-trichlorophenol (tcp). all of the gene products except tcpy have assigned functions in tcp metabolism. sequence comparison identified tcpy as a member of cog4313, a group of hypothetical proteins. tcpy has a signal peptide, indicating it is a membrane or secreted protein. secondary structure and topology analysis indicated tcpy as a beta-barrel outer membrane protein, similar to the escherichia coli outer ... | 2010 | 19937267 |
| epidemiology of bloodstream infections in patients with haematological malignancies with and without neutropenia. | all bacterial isolates from 7058 patients admitted to haemato-oncology wards at national taiwan university hospital between 2002 and 2006 were characterized. in total 1307 non-duplicate bloodstream isolates were made from all patients with haematological malignancy; 853 (65%) of these were from neutropenic patients. gram-negative bacteria predominated (60%) in neutropenic isolates with escherichia coli (12%), klebsiella pneumoniae (10%), acinetobacter calcoaceticus-baumannii complex (6%), and st ... | 2010 | 19941686 |
| bacteremia due to acinetobacter genomic species 10. | six patients with acinetobacter genomic species 10 bacteremia were identified. the clinical features of the patients, phenotypic and genotypic identifications, antimicrobial susceptibilities, and genes flanking isaba1 of the bacteria were described. the results revealed that this bacterium is a potentially lethal pathogen that can cause health care-associated infections in debilitated patients. | 2010 | 19955266 |
| isolation of hydrocarbon degrading bacteria from soils contaminated with crude oil spills. | a feasibility study was conducted to evaluate the capability of bacterial strains to degrade crude oil under in vitro conditions. pseudomonas strain ps-i could degrade alkanes (70.69%) and aromatics (45.37%). alkanes and aromatic fractions separated by column chromatography were analyzed by gas chromatography. in case of pseudomonas strain ps-i, nc17/pr, nc18/ph ratios decreased from 2.5100 to 0.1232 and from 7.2886 to 0.3853, respectively. it was concluded that out of the isolated strains, pseu ... | 2009 | 19957890 |
| structure, mechanism, and substrate profile for sco3058: the closest bacterial homologue to human renal dipeptidase . | human renal dipeptidase, an enzyme associated with glutathione metabolism and the hydrolysis of beta-lactams, is similar in sequence to a cluster of approximately 400 microbial proteins currently annotated as nonspecific dipeptidases within the amidohydrolase superfamily. the closest homologue to the human renal dipeptidase from a fully sequenced microbe is sco3058 from streptomyces coelicolor. dipeptide substrates of sco3058 were identified by screening a comprehensive series of l-xaa-l-xaa, l- ... | 2010 | 20000809 |
| detection of kpc in acinetobacter spp. in puerto rico. | during an island-wide pcr-based surveillance study of beta-lactam resistance in multidrug-resistant (mdr) escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, and acinetobacter calcoaceticus-baumannii complex isolates obtained from 17 different hospitals, 10 kpc-positive acinetobacter isolates were identified. dna sequencing of the bla(kpc) gene identified kpc-2, -3, and -4 and a novel variant, kpc-10. this is the first report of a kpc-type beta-lactamase identified in acinetobacter ... | 2010 | 20038618 |
| access technique and its problems in parenteral nutrition - guidelines on parenteral nutrition, chapter 9. | catheter type, access technique, and the catheter position should be selected considering to the anticipated duration of pn aiming at the lowest complication risks (infectious and non-infectious). long-term (>7-10 days) parenteral nutrition (pn) requires central venous access whereas for pn <3 weeks percutaneously inserted catheters and for pn >3 weeks subcutaneous tunnelled catheters or port systems are appropriate. cvc (central venous catheter) should be flushed with isotonic nacl solution bef ... | 2009 | 20049083 |
| genome organization and characterization of the virulent lactococcal phage 1358 and its similarities to listeria phages. | virulent phage 1358 is the reference member of a rare group of phages infecting lactococcus lactis. electron microscopy revealed a typical icosahedral capsid connected to one of the smallest noncontractile tails found in a lactococcal phage of the siphoviridae family. microbiological characterization identified a burst size of 72 virions released per infected host cell and a latent period of 90 min. the host range of phage 1358 was limited to 3 out of the 60 lactococcal strains tested. moreover, ... | 2010 | 20061452 |
| human pathogens abundant in the bacterial metagenome of cigarettes. | many studies have evaluated chemical, heavy metal, and other abiotic substances present in cigarettes and their roles in the development of lung cancer and other diseases, yet no studies have comprehensively evaluated bacterial diversity of cigarettes and the possible impacts of these microbes on respiratory illnesses in smokers and exposed nonsmokers. | 2010 | 20064769 |
| human pathogens abundant in the bacterial metagenome of cigarettes. | many studies have evaluated chemical, heavy metal, and other abiotic substances present in cigarettes and their roles in the development of lung cancer and other diseases, yet no studies have comprehensively evaluated bacterial diversity of cigarettes and the possible impacts of these microbes on respiratory illnesses in smokers and exposed nonsmokers. | 2010 | 20064769 |
| acute spinal cord injury and infection with multidrug-resistant acinetobacter calcoaceticus-baumannii complex among returning operation iraqi freedom soldiers: successful innovations in rehabilitation during isolation. | concerns about drug-resistant infectious organisms are increasing in rehabilitation facilities. resulting isolation protocols can potentially challenge the patients' access to medical care, psychological adaptation, mobility, and environmental interaction and therefore hinder the rehabilitation process. we report a systematic, retrospective case review of an active-duty army sergeant who sustained a c5 american spinal cord injury association impairment scale a spinal cord injury while serving in ... | 2010 | 20068440 |
| aminoglycoside resistance and susceptibility testing errors in acinetobacter baumannii-calcoaceticus complex. | antimicrobial resistance is depleting the pharmacopeia of agents clinically useful against gram-negative bacilli. as the number of active agents diminishes, accurate susceptibility testing becomes critical. we studied the susceptibilities of 107 isolates of the acinetobacter baumannii-calcoaceticus complex to amikacin, gentamicin, and tobramycin using disk diffusion, etest, as well as the phoenix, vitek 2, and microscan automated systems, and compared the results to those obtained by broth micro ... | 2010 | 20107089 |
| switching catalysis from hydrolysis to perhydrolysis in pseudomonas fluorescens esterase. | many serine hydrolases catalyze perhydrolysis, the reversible formation of peracids from carboxylic acids and hydrogen peroxide. recently, we showed that a single amino acid substitution in the alcohol binding pocket, l29p, in pseudomonas fluorescens (sik wi) aryl esterase (pfe) increased the specificity constant of pfe for peracetic acid formation >100-fold [bernhardt et al. (2005) angew. chem., int. ed. 44, 2742]. in this paper, we extend this work to address the three following questions. fir ... | 2010 | 20112920 |
| comparison of traditional phenotypic identification methods with partial 5' 16s rrna gene sequencing for species-level identification of nonfermenting gram-negative bacilli. | correct identification of nonfermenting gram-negative bacilli (nfb) is crucial for patient management. we compared phenotypic identifications of 96 clinical nfb isolates with identifications obtained by 5' 16s rrna gene sequencing. sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify nfb. | 2010 | 20164273 |
| exploring the evolutionary dynamics of plasmids: the acinetobacter pan-plasmidome. | prokaryotic plasmids have a dual importance in the microbial world: first they have a great impact on the metabolic functions of the host cell, providing additional traits that can be accumulated in the cell without altering the gene content of the bacterial chromosome. additionally and/or alternatively, from a genome perspective, plasmids can provide a basis for genomic rearrangements via homologous recombination and so they can facilitate the loss or acquisition of genes during these events, w ... | 2010 | 20181243 |
| sepsis-related mortality of very low birth weight brazilian infants: the role of pseudomonas aeruginosa. | the aim of this study is to identify risk factors for sepsis-related mortality in low birth weight (<1500 g) infants. we performed retrospective cohort study to investigate risk factors for sepsis-related mortality in all neonates birth weight <1500 g admitted to level iii neonatal intensive care unit, brazil, april 2001/september 2004. of the 203 cases, 71 (35%) had sepsis. of those, gram-positive was identified in 52/87 blood cultures (59.8%), the most common coagulase-negative staphylococcus ... | 2010 | 20182631 |
| sepsis-related mortality of very low birth weight brazilian infants: the role of pseudomonas aeruginosa. | the aim of this study is to identify risk factors for sepsis-related mortality in low birth weight (<1500 g) infants. we performed retrospective cohort study to investigate risk factors for sepsis-related mortality in all neonates birth weight <1500 g admitted to level iii neonatal intensive care unit, brazil, april 2001/september 2004. of the 203 cases, 71 (35%) had sepsis. of those, gram-positive was identified in 52/87 blood cultures (59.8%), the most common coagulase-negative staphylococcus ... | 2010 | 20182631 |
| heterotrophic nitrogen removal by a newly isolated acinetobacter calcoaceticus hnr. | strain hnr, isolated from a membrane bioreactor (mbr), demonstrates a surprising ability to convert ammonium to nitrogen gas under aerobic conditions while growing heterotrophically. on the basis of phylogenetic analysis of the 16s rrna gene sequence, strain hnr was related to acinetobacter calcoaceticus (98.9% identity). nitrogen balance during heterotrophic growth with 120mg/l of nh(4)(+)-n showed that 40.2% of nh(4)(+)-n was in the form of n(2) and 52.1% was found in biomass. only a trace pro ... | 2010 | 20202830 |
| biological treatment of waste gas containing mixture of monochlorobenzene (mcb) and benzene in a bench scale biofilter. | the paper outlines treatment of waste gas containing monochlorobenzene (mcb) and benzene in a mixture using biofilter packed with compost and woodchips seeded with acinetobacter calcoaceticus. the biofilter could treat waste gas containing mcb and benzene effectively with an efficiency of (99+/-5%) and (97+/-6%) at optimal empty bed contact time (ebct) of 3 min with a loading of 57 g/m(3)/h of mcb and 2g/m(3)/h of benzene. at optimum loading of mcb and benzene, the biofilter showed total bacteri ... | 2010 | 20202831 |
| rapid differentiation of francisella species and subspecies by fluorescent in situ hybridization targeting the 23s rrna. | francisella (f.) tularensis is the causative agent of tularemia. due to its low infectious dose, ease of dissemination and high case fatality rate, f. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. microbiological diagnosis is cumbersome and time-consuming. methods for the direct detection of the pathogen (immunofluorescence, pcr) have been developed but are restricted to reference laboratories. | 2010 | 20205957 |