Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| insertional specificity of transposon tn5 in acinetobacter sp. | suicide plasmid pjb4ji, containing transposon tn5 and phage mu, was introduced from escherichia coli 1830 into acinetobacter sp. strain ho1-n and acinetobacter calcoaceticus bd413. kanamycin-resistant (kmr) exconjugants of ho1-n and bd413, isolated on complex medium, were screened for auxotrophic requirements. over 10,000 kmr clones were examined, but no auxotrophs were detected. several kmr exconjugants of bd413 and ho1-n, obtained from independent matings, were chosen for further study. all tn ... | 1984 | 6319369 |
| the absence of quinoprotein alcohol dehydrogenase in acinetobacter calcoaceticus. | it is shown that the unusual nad(p)+-independent quinoprotein alcohol dehydrogenase, said previously to be responsible for oxidation of ethanol during growth of acinetobacter calcoaceticus lmd 79.39, was in fact isolated from an unidentified organism which contained cytochrome c and which has now been lost. several genuine strains of a. calcoaceticus do not contain cytochrome c nor do they contain a quinoprotein alcohol dehydrogenase. the enzyme responsible for ethanol oxidation in these bacteri ... | 1983 | 6317794 |
| [in vitro bacteriostatic activity of cefmenoxime (sce 1365), cefotaxime and moxalactam]. | the in vitro activity of cefmenoxime (sce 1365), a new cephalosporin derivate was compared with two other "third generation" cephalosporins: cefotaxime and moxalactam. cefmenoxime as cefotaxime and moxalactam were very active against 305 cephalosporinase-producing and cephalosporinase-non-producing enterobacteriaceae. cefmenoxime was the most active against serratia marcescens, citrobacter freundii, morganella morganii, salmonella, shigella and yersinia enterocolitica with a mean mic at least tw ... | 1983 | 6312395 |
| susceptibility of 324 nonfermentative gram-negative rods to 6 cephalosporins and azthreonam. | susceptibility of 324 isolates of nonfermentative gram-negative bacteria to cephalothin, cefamandole, cefoxitin, ceftazidime, cefsulodin, and azthreonam was determined by agar dilution and disc diffusion techniques. with the exception of moraxella species, first- and second-generation cephalosporins were minimally active against nonfermenters tested. cefsulodin and azthreonam were mainly active against pseudomonas aeruginosa. in contrast, ceftazidime and ceftriaxone exhibited wider activity spec ... | 1983 | 6311492 |
| pseudomonas aeruginosa and acinetobacter calcoaceticus: in vitro susceptibility of 150 clinical isolates to five beta-lactam antibiotics and tobramycin. | the in vitro activities of azlocillin, carbenicillin, ceftriaxone, piperacillin, n-formimidoyl thienamycin (n-f thienamycin) and tobramycin have been compared against clinical isolates of pseudomonas aeruginosa (n = 100) and acinetobacter calcoaceticus (n = 50). an agar dilution method was employed for measurement of minimal inhibitory concentration (mic). tobramycin was the most active drug against p. aeruginosa (mic less than or equal to 2 mg/l). of the beta-lactam antibiotics, n-f thienamycin ... | 1983 | 6311491 |
| [activity of sulbactam and clavulanic acid, alone and combined, on acinetobacter calcoaceticus]. | sulbactam and clavulanic acid, both beta-lactamase inhibitors, were studied alone or associated on 85 isolates of acinetobacter calcoaceticus. when sulbactam was tested alone the minimal inhibitory concentration (mic) was less than 2 micrograms/ml with the carbenicillin-sensitive strains of a. calcoaceticus and between 8 to 16 micrograms/ml with the carbenicillin-resistant strains. in the presence of clavulanic acid (5 or 10 micrograms/ml) the mic of sulbactam on all the carbenicillin-resistant ... | 1983 | 6307103 |
| is15, a new insertion sequence widely spread in r plasmids of gram-negative bacteria. | we have shown that the is15 element, first detected in salmonella ordonez and previously designated is1522 (labigne-roussel et al. 1981), could transpose, with an approximate frequency of 5 x 10(-5), to various sites of different replicons in an escherichia coli host deficient for general homologous recombination. physical mapping with restriction endonucleases of this 1,500 base pairs (bp) transposable module indicated the presence of two, possibly contiguous, directly repeated internal sequenc ... | 1983 | 6304459 |
| transposon-directed mutagenesis and chromosome mobilization in acinetobacter calcoaceticus ebf65/65. | 1983 | 6301938 | |
| comparative activity of cefotaxime and selected beta-lactam antibiotics against haemophilus influenzae and aerobic gram-negative bacilli. | the activity of cefotaxime was compared with that of ampicillin, moxalactam, and cefoperozone against 50 isolates of haemophilus influenzae and with that of ampicillin, carbenicillin, cephalothin, cefoxitin, cefamandole, cefazolin, and several other established and investigational beta-lactam antibiotics against several hundred isolates of gram-negative aerobic enteric bacilli. minimal inhibitory concentrations of the drugs were determined by the agar plate dilution technique for h. influenzae a ... | 1982 | 6294790 |
| cefotaxime: a review of in vitro antimicrobial properties and spectrum of activity. | cefotaxime has remarkable potency against all enterobacteriaceae, including enterobacter species, citrobacter freundii, serratia marcescens, and morganella morganii, proteus vulgaris, and providencia species--all of which are resistant to earlier cephalosporins. cefotaxime generally inhibits greater than 90% of enteric bacilli at concentrations of less than or equal to 0.5 microgram/ml; in one study it inhibited greater than 98% of isolates at less than or equal to 8 micrograms/ml. for staphyloc ... | 1982 | 6294779 |
| ceftriaxone: in vitro studies and clinical evaluation. | the in vitro activity of ceftriaxone against 437 clinical isolates of gram-negative bacilli was determined. ceftriaxone was found to have high in vitro activity against enterobacteriaceae, with the exception of enterobacter cloacae. ceftriaxone was only minimally active against pseudomonas aeruginosa and acinetobacter calcoaceticus. we evaluated the clinical efficacy and toxicity of ceftriaxone in 55 adult patients. bacterial infection was confirmed by the isolation of etiological bacteria in 30 ... | 1982 | 6289734 |
| [in vitro activity of ceftazidime compared with five beta-lactamase stable compounds against clinical strains of acinetobacter calcoaceticus ]. | acinetobacter calcoaceticus is recognized as one of the most resistant nosocomial pathogens. clinical isolates of acinetobacter are usually resistant to most beta-lactam antibiotics. the objective of this study was to evaluate the in vitro activity of ceftazidime, a new broad spectrum highly potent beta-lactam antibiotic, able to inhibit especially pseudomonas and providencia. its activity against 96 clinical strains of acinetobacter was compared with the activity of 5 recent beta-lactam antibio ... | 1982 | 6289227 |
| enzymatic adenylylation of spectinomycin by acinetobacter calcoaceticus subsp. anitratus. | spectinomycin (spc) was inactivated in the presence of adenosine-5'-triphosphate and magnesium ion by an enzyme preparation made from acinetobacter calcoaceticus subsp. anitratus gn12313 resistant to both spc and streptomycin. the structure of the inactivated spc was found to be the adenylylated product of the hydroxy group on c-9 of the actinamine moiety. | 1981 | 6270047 |
| in vitro antimicrobial activity of ceftizoxime against glucose-nonfermentative gram-negative rods. | ceftizoxime, a new cephalosporin, was active against pseudomonas cepacia, flavobacterium meningosepticum, alcaligenes faecalis, and acinetobacter calcoaceticus and was more potent against pseudomonas aeruginosa and pseudomonas putida than was carbenicillin. | 1981 | 6269480 |
| properties of six pesticide degradation plasmids isolated from alcaligenes paradoxus and alcaligenes eutrophus. | biophysical and genetic properties of six independently isolated plasmids encoding the degradation of the herbicides 2,4-dichlorophenoxyacetic acid and 4-chloro-2-methylphenoxyacetic acid are described. four of the plasmids, pjp3, pjp4, pjp5, and pjp7, had molecular masses of 51 megadaltons, belonged to the incp1 incompatibility group, and transferred freely to strains of escherichia coli, rhodopseudomonas sphaeroides, rhizobium sp., agrobacterium tumefaciens, pseudomonas putida, pseudomonas flu ... | 1981 | 6257648 |
| [quantitative immunological method for determining rubredoxin in crude extracts of acinetobacter calcoaceticus]. | 1980 | 6246689 | |
| norfloxacin (mk-0366, am-715): in vitro activity and cross-resistance with other organic acids including quality control limits for disk diffusion testing. | norfloxacin is a new organic acid antimicrobic with an expanded spectrum that includes staphylococcus spp, most pseudomonas spp, acinetobacter calcoaceticus ssp anitratus, some streptococci, as well as nearly all enteric bacilli. the drug appears to be bactericidal. its expanded spectrum suggests that it should be tested separately by disk diffusion or other susceptibility testing methods. tentative norfloxacin disk test quality control limits were derived from the results of a multi-laboratory ... | 1983 | 6232084 |
| in vitro activity of ciprofloxacin, norfloxacin and nalidixic acid. | the in vitro antibacterial activity of the new quinoline derivative ciprofloxacin (bay 0 9867) was evaluated in comparison to norfloxacin and nalidixic acid using 495 clinical strains of gram-negative and gram-positive bacteria. the compound was highly active against enterobacteriaceae, with mics ranging from 0.008 mg/l to 4 mg/l, whereas the mics of norfloxacin ranged from 0.03 mg/l to 16 mg/l. all strains of pseudomonas aeruginosa and acinetobacter calcoaceticus were inhibited with a concentra ... | 1983 | 6222896 |
| moxalactam therapy of bacterial meningitis in adults. | the therapeutic efficacy and attainable cerebrospinal fluid (csf) concentrations of moxalactam, administered by intravenous drip in a dose of 2 g every 4 to 8 h, were evaluated in seven adult patients with bacterial meningitis. streptococcus pneumoniae, staphylococcus aureus, pseudomonas aeruginosa, and acinetobacter calcoaceticus var. anitratus, each caused infection in four patients, whereas escherichia coli was the cause of infection in the other three patients. the mean moxalactam concentrat ... | 1983 | 6220673 |
| in vitro antibacterial activity of norfloxacin (mk-0366). | the in vitro activity of norfloxacin (mk-0366) compared with that of beta-lactam antibiotics and, where appropriate of gentamicin or metronidazole was assessed against recent clinical isolates of common bacteria. the compound was highly active against most enterobacteria (minimal inhibitory concentrations [mics], 0.008 to 32 micrograms/ml; 90% inhibited by 0.25 micrograms/ml), haemophilus influenzae (mics, 0.03 to 0.12 micrograms/ml), and neisseria gonorrhoeae (mics, 0.008 to 0.016 micrograms/ml ... | 1982 | 6211139 |
| studies on the utilization of octane by algae. | the objective of this study was to investigate the influence of various octane concentrations on a mixed culture of scenedesmus obliquus, chlorella vulgaris and oscillatoria sp. and the ability of these algae to utilize octane as the sole source of organic carbon in the medium. the same investigations were carried out for a mixed culture consisting of algae and the strain acinetobacter calcoaceticus, and for a. calcoaceticus alone. the results obtained show that all of the strains under study, b ... | 1983 | 6196951 |
| disinfection of respirator tubing: a comparison of chemical versus hot water machine-assisted processing. | a comparison of machine-assisted chemical disinfection, using a glutaraldehyde solution, and machine-assisted hot water disinfection was made by placing a large inoculum (approximately 10(7) colonies of pseudomonas aeruginosa or acinetobacter calcoaceticus var. anitratus) in sets of tubing before processing. machine-assisted chemical processing proved to be the most efficient method with a disinfection failure rate of 6 per cent (two positive tubes out of 35). machine-assisted hot water processi ... | 1983 | 6195230 |
| meningitis due to acinetobacter calcoaceticus variant anitratus. | 1980 | 6182240 | |
| acinetobacter calcoaceticus: some approaches to a problem. | 1981 | 6174586 | |
| acinetobacter spp.: distinct morphology on eosin methylene blue agar as an aid to identification in drinking water. | acinetobacter calcoaceticus, frequently found in drinking waters and implicated in nosocomial infections, was presumptively identified by its tiny, blue colonial appearance on levine eosin methylene blue agar. all of the 33 isolates from drinking water showing this distinctive colonial appearance were identified as a. calcoaceticus. | 1981 | 6165324 |
| clues from xanthomonas campestris about the evolution of aromatic biosynthesis and its regulation. | the recent placement of major gram-negative prokaryotes (superfamily b) on a phylogenetic tree (including, e.g., lineages leading to escherichia coli, pseudomonas aeruginosa, and acinetobacter calcoaceticus) has allowed initial insights into the evolution of the biochemical pathway for aromatic amino acid biosynthesis and its regulation to be obtained. within this prokaryote grouping, xanthomonas campestris atcc 12612 (a representative of the group v pseudomonads) has played a key role in facili ... | 1984 | 6152589 |
| phh502, a plasmid with incp and inci alpha characters, loses the latter by a specific reca-independent deletion event. | plasmid phh502, of molecular weight 70 x 10(6), determined resistance to tetracycline, chloramphenicol, trimethoprim, sulphonamides and mercuric chloride and was incompatible with members of incp and inci alpha. it resembled other plasmids of inci alpha in the following properties: it determined pili that were morphologically and serologically i alpha pili, whose production was repressed in established plasmid-carrying (r+) cultures; its transfer was equally efficient in liquid or on solid mediu ... | 1982 | 6130120 |
| [gamma-glutamyl-transferase activity in the family "neisseriaceae" (author's transl)]. | members of the family neisseriaceae (554 strains) were screened for gamma-glutamyl-transferase (gamma gt) activity. gamma gt was produced by neisseria meningitidis but not produced by any strain of n. gonorrhoeae, n. lactamica and branhamella catarrhalis. most non-proteolytic acinetobacter calcoaceticus produced gamma gt whereas most a. lwoffii failed to produce gamma gt. | 1982 | 6125120 |
| sensitivity of acinetobacter calcoaceticus strains to seven cephalosporins. | 1981 | 6115226 | |
| acinetobacter calcoaceticus causing subacute bacterial endocarditis. | 1981 | 6114359 | |
| transposon-mediated multiple antibiotic resistance in acinetobacter strains. | acinetobacter calcoaceticus subsp. anitratus, which is unusually resistant to multiple antibiotics, was the cause of an epidemic of respiratory tract infections in patients in an intensive care unit. a representative isolate of the epidemic strain was found to contain the aminoglycoside-modifying enzymes 3-n-acetyltransferase, 3'-phosphotransferase, and 3"-adenylyltransferase, which confer resistance to gentamicin, kanamycin, and streptomycin, respectively. in addition, the strain produced a cep ... | 1982 | 6100428 |
| regulation of the enzymes of the beta-ketoadipate pathway in moraxella calcoacetica. 1. general aspects. | 1967 | 6060184 | |
| regulation of the enzymes of the beta-ketoadipate pathway in moraxella calcoacetica. 3. effects of 3-hydroxy-4-methylbenzoate on the synthesis of enzymes of the protocatechuate branch. | 1968 | 5650850 | |
| regulation of the enzymes of the beta-ketoadipate pathway in moraxella calcoacetica. 4. constitutive synthesis of beta-ketoadipate succinyl-coa transferases ii and 3. | 1968 | 5645526 | |
| regulation of the enzymes of the beta-ketoadipate pathway in moraxella calcoacetica. 2. the role of protocatechuate as inducer. | 1968 | 5645525 | |
| comparison of the two isofunctional enol-lactone hydrolases from acinetobacter calcoaceticus. | the rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (elh i and elh ii) of acinetobacter calcoaceticus were determined. the molecular weights of elh i and elh ii were found by gel filtration to be approximately 24,000 and 21,000, respectively. in crude extract at 45 c the two enzymes showed a marked difference in rate of thermal denaturation. after chromatography on sephadex g-100, however, the rates were nearly identical. the thermolabilit ... | 1971 | 5573730 |
| production of phospholipase c in acinetobacter calcoaceticus. | 1971 | 5290406 | |
| phospholipase activity of acinetobacter calcoaceticus. | 1971 | 5283982 | |
| haemolytic activity of acinetobacter calcoaceticus. | 1971 | 5280419 | |
| mapping of the tryptophan genes of acinetobacter calcoaceticus by transformation. | auxotrophs of acinetobacter calcoaceticus blocked in each reaction of the synthetic pathway from chorismic acid to tryptophan were obtained after n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis. one novel class was found to be blocked in both anthranilate and p-aminobenzoate synthesis; these mutants (trpg) require p-aminobenzoate or folate as well as tryptophan (or anthranilate) for growth. the loci of six other auxotrophic classes requiring only tryptophan were defined by growth, accumulation, ... | 1972 | 5086660 |
| effect of growth rate and nutrient limitation on the composition and biomass yield of acinetobacter calcoaceticus. | acinetobacter calcoaceticus was grown on ethanol in a chemostat as a model system for single-cell protein production. the substrate yield coefficient (y(s), grams of biomass/gram of ethanol), protein yield coefficient (y(p), grams of protein/gram of ethanol), and biomass composition were measured as a function of the specific growth rate. nucleic acid, protein, y(p), and y(s) all increased at higher growth rates. although protein content increased only 14% (from 53 to 67%), y(p) almost doubled o ... | 1974 | 4844268 |
| purification and properties of l-asparaginase a from acinetobacter calcoaceticus. | 1973 | 4770738 | |
| role of carbohydrates in the metabolism of acinetobacter calcoaceticus. | 1973 | 4748364 | |
| growth of acinetobacter calcoaceticus on ethanol. | a soil microorganism, identified as acinetobacter calcoaceticus, was cultivated on ethanol as a sole source of carbon. this organism grew with a maximum specific growth rate of 0.7/h. the ph optimum for growth was between 6.5 and 7.5, and the temperature optimum was between 32 and 35 c. ethanol metabolism by this organism was inducible by ethanol, and the presence of acetate led to the repression of ethanol dehydrogenase. at higher cell densities the cessation of growth on ethanol was accompanie ... | 1973 | 4715559 |
| acute villous inflammation in the placenta following intrauterine transfusion. | infection is well recognized as a complication of intrauterine transfusion. the majority of cases are fortunately mild and consist merely of chorio-amnionitis. the present case, of severe type, resulted from contamination of the donor blood with acinetobacter calcoaceticus. spread of infection from foetus to mother has been carefully studied and an entirely new type of lesion in the placenta described. this takes the form of acute villous inflammation with resultant micro-abscess formation benea ... | 1972 | 4646299 |
| properties of purified phospholipase c from acinetobacter calcoaceticus. | 1972 | 4630254 | |
| leukolytic activity of acinetobacter calcoaceticus. | 1972 | 4630253 | |
| multiple dna polymerases in acinetobacter calcoaceticus. | 1974 | 4604798 | |
| dna polymerase i from acinetobacter calcoaceticus: enzymatic properties. | 1974 | 4452356 | |
| the effect of imidoesters on the protocatechuate 3,4-dioxygenase activity of acinetobacter calcoaceticus. | 1974 | 4419544 | |
| [regulation of citrate synthase activity in acinetobacter calcoaceticus]. | 1974 | 4371720 | |
| pyrimidine metabolism in acinetobacter calcoaceticus. | the metabolism of thymine, thymidine, uracil, and uridine has been investigated in five different strains of acinetobacter calcoaceticus. attempts to isolate thymine and thymidine auxotrophic mutants were not successful. consistent with this finding was the observation that uptake of radioactive thymine or thymidine could not be demonstrated. search for enzymes capable of transforming thymine via thymidine to thymidine-5'-monophosphate in crude extracts was performed, and the following enzymes w ... | 1973 | 4355484 |
| anthranilate synthetase of acinetobacter calcoaceticus. separation and partial characterization of subunits. | 1973 | 4349869 | |
| the nature of phospholipase c from acinetobacter calcoaceticus: effects on whole red cells and red cell membranes. | 1973 | 4271908 | |
| relationships among enzymes of the beta-ketoadipate pathway. iv. muconolactone isomerase from acinetobacter calcoaceticus and pseudomonas putida. | 1974 | 4215816 | |
| regulation of the synthesis of enzymes of tryptophan dissimilation in acinetobacter calcoaceticus. | 1972 | 4117534 | |
| versatile mercury-resistant cloning and expression vectors. | cloning vectors have been constructed employing two diverse replicons, incq and p15a. both vectors confer resistance to kanamycin (km) and mercuric ions (hg2+). one of these vectors, pdg105, is a broad-host-range, nonconjugative, oligocopy incq plasmid, which is capable of transforming escherichia coli, acinetobacter calcoaceticus, and pseudomonas putida. the second vector, pdg106, is a narrow-host-range, multicopy cloning vector compatible with pbr322. both vectors contain unique cloning sites ... | 1985 | 4092936 |
| interconvertible molecular-weight forms of the bifunctional chorismate mutase-prephenate dehydratase from acinetobacter calcoaceticus. | acinetobacter calcoaceticus belongs to a large phylogenetic cluster of gram-negative procaryotes that all utilize a bifunctional p-protein (chorismate mutase-prephenate dehydratase) [ec 5.4.99.5-4.2.1.51] for phenylalanine biosynthesis. these two enzyme activities from ac. calcoaceticus were inseparable by gel-filtration or deae-cellulose chromatography. the molecular weight of the p-protein in the absence of effectors was 65,000. in the presence of l-tyrosine (dehydratase activator) or l-phenyl ... | 1985 | 4083897 |
| [comparison of the in vitro activity of amikacin, netilmicin, piperacillin and moxalactam against acinetobacter calcoaceticus var. anitratum]. | 1985 | 4079560 | |
| an underestimated nosocomial pathogen, acinetobacter calcoaceticus. | 1985 | 4077770 | |
| identification of 2-keto-3-deoxy-1,7-dicarboxyheptonic acid as a constituent of the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305. | a 3-deoxyaldulosonic acid, not previously described, was identified as a component of the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305: 2-keto-3-deoxy-1,7-dicarboxyheptonic acid. this compound, whose steric configuration has not been determined, was released from lipopolysaccharide after acid hydrolysis as monosaccharide and in the form of a glucose-substituted disaccharide. it was identified by combined gas-liquid chromatography/mass spectrometry using various derivatization pro ... | 1985 | 4076175 |
| endemic nosocomial acinetobacter calcoaceticus bacteremia. clinical significance, treatment, and prognosis. | the medical records of 27 patients with blood cultures positive for acinetobacter calcoaceticus over a recent five-year period (0.7% of all positive blood cultures) were reviewed retrospectively to determine the epidemiologic and clinical significance of these isolates. eighteen isolates represented true bacteremias, 16 of which were hospital acquired. patients most frequently were located in an intensive care unit or on a surgical ward. a seasonal july-to-september peak incidence was noted. the ... | 1985 | 4074029 |
| a 28,000-dalton protein of normal mouse serum binds specifically to the inner core region of bacterial lipopolysaccharide. | normal mouse serum was found to contain a protein, referred to here as factor, which binds to the inner core region of lipopolysaccharides (lpss) of various bacterial families. since factor-lps interactions resulted in activation of guinea pig complement, factor activity could be assayed by a passive hemolysis test with sheep erythrocytes coated with lps or lipid a from acinetobacter calcoaceticus (which was found earlier to bind particularly well to factor). factor was purified by g-50 and hydr ... | 1985 | 4066028 |
| structural studies of the capsular polysaccharide of acinetobacter calcoaceticus bd4. | compositional analysis of the intact and carboxyl-reduced capsular polysaccharide of acinetobacter calcoaceticus bd4 (ps-4) showed it to consist of l-rhamnose, d-glucose, d-glucuronic and d-mannose in molar ratios of 4:1:1:1. 13c-nuclear magnetic resonance spectroscopy, methylation analysis, oligosaccharide analysis and base-catalyzed beta-elimination were used to elucidate the primary structure. oligosaccharides were obtained by enzymatic depolymerization with a specific bacteriophage-induced d ... | 1985 | 4054115 |
| synergistic necrotizing fasciitis: a case of polymicrobic infection with acinetobacter calcoaceticus. | 1985 | 4053677 | |
| replacement of methoxatin by 4,7-phenanthroline-5,6-dione and the inability of other phenanthroline quinones, as well as 7,9-di-decarboxy methoxatin, to serve as cofactors for the methoxatin-requiring glucose dehydrogenase of acinetobacter calcoaceticus. | glucose dehydrogenase from a. calcoaceticus has been dissociated into apoenzyme and methoxatin coenzyme, and enzyme activity restored by replacing coenzyme with 4,7-phenanthroline-5,6-dione but not with 1,10- nor 1,7-phenanthroline-5,6-diones nor with 7,9-decarboxy methoxatin. | 1985 | 4052066 |
| regions of broad-host-range plasmid rk2 involved in replication and stable maintenance in nine species of gram-negative bacteria. | the replication and maintenance properties of the broad-host-range plasmid rk2 and its derivatives were examined in nine gram-negative bacterial species. two regions of rk2, the origin of replication (oriv) and a segment that encodes for a replication protein (trfa delta kild, designated trfa*), are sufficient for replication in all nine species tested. however, stable maintenance of this minimal replicon (less than 0.3% loss per generation under nonselection conditions) is observed only in esch ... | 1985 | 4044529 |
| [acinetobacter calcoaceticus infection--bacteriological and clinical studies of septicemia]. | 1985 | 4032800 | |
| [utilization of 3-chlorobenzoic acid by acinetobacter calcoaceticus]. | a strain of acinetobacter calcoaceticus assimilating 3-chlorobenzoic acid (3-cba) as a sole source of carbon and energy was isolated from soil near moscow. when 3-cba is utilized, 2-chloro-cis, cis-muconic acid is accumulated in the medium. the liberation of chlorine atoms is 50--60% of the theoretically possible value. the oxidation of 3-cba yields 3-chloropyrocatechol (3-cpc) and 4-chloropyrocatechol (4-cpc). 4-cpc serves as a source of nutrition for a. calcoaceticus. the oxidation of 3-cpc yi ... | 1985 | 4010553 |
| in-vitro and in-vivo activities of antimicrobial agents against acinetobacter calcoaceticus. | the virulence of clinical isolates of acinetobacter calcoaceticus subsp. anitratus was studied in mice, and in-vitro and in-vivo activities of several antimicrobial agents were evaluated. in in-vitro susceptibility testings, tetracyclines, aminoglycosides, and peptide were highly sensitive, and minocycline and doxycycline were the most active of 21 antibiotics tested against 84 clinical isolates of acin. calcoaceticus. virulence tests for mice revealed that some strains exhibited high virulence ... | 1985 | 4008377 |
| acinetobacter calcoaceticus septicemia in patients with cancer. | at our institution, 95 cases of acinetobacter septicemia occurred over a ten-year period (1973 to 1982) in patients being treated for cancer. in 24 patients the infection was polymicrobial, while acinetobacter ssp was the only offending pathogen in 71 patients. in 76 patients (80%), the infection was related to an indwelling central venous catheter (cvc). a sharp increase in the frequency of acinetobacter septicemia was noticed in the years 1981 and 1982 and coincided with a marked increase in t ... | 1985 | 4001997 |
| enzymatic profile of clinical isolates of acinetobacter calcoaceticus. | the enzymatic profiles of 109 clinical isolates of acinetobacter calcoaceticus subsp. anitratus and lwoffi were determined with conventional plate tests and the rapid api zym system (analytab products, plainview, n.y.). the majority of strains tested lacked dnase, hemolysin, protease, elastase and gelatinase. strong enzymatic activities of butyrate esterase (c4), caprylate esterase (c8) and leucine arylamidase were detected in all isolates. no trypsin, chymotrypsin, alkaline phosphatase or gluco ... | 1985 | 4000064 |
| transferable amikacin resistance in pseudomonas maltophilia and acinetobacter calcoaceticus. | 1985 | 3997255 | |
| assessment of head-space gas-liquid chromatography for the rapid detection of growth in blood cultures. | blood for transfusion was inoculated with between 10(0) and 10(2) colony-forming units (cfu) per ml of each of 59 microbial isolates and added to cooked meat broth. at intervals up to 72 h incubation, the cultures were examined by conventional visual inspection and automated head-space gas-liquid chromatography (hs-glc). forty-six isolates including all those examined of staphylococcus aureus, streptococcus pyogenes, s. pneumoniae, s. faecalis, s. milleri, s. mitior, s. mitis, s. salivarius, s. ... | 1985 | 3988854 |
| isolation of acinetobacter calcoaceticus from a cow with mastitis. | 1985 | 3976314 | |
| an outbreak of infections with acinetobacter calcoaceticus in burn patients: contamination of patients' mattresses. | during a 21-month period acinetobacter calcoaceticus was the most common organism causing infections in a university burn center. forty-three of 103 patients admitted became infected with this organism. risk factors associated with burn wound colonization with acinetobacter included larger burns and foley catheter use; however, only a longer duration of hospitalization was an independent discriminator of colonization. infection-control measures, including strict isolation and closure and repaint ... | 1985 | 3968451 |
| transformation and mobilization of cloning vectors in acinetobacter spp. | r300b-, rsf1010-, and rk2-derived plasmids were introduced into acinetobacter sp. strain ho1-n and acinetobacter calcoaceticus bd413 by transformation and conjugal mobilization. the transformation frequencies of bd413 were 4.2 x 10(6) to 6.3 x 10(6) transformants per micrograms of dna per 10(9) recipient cells. conjugal mobilization frequencies were 1.1 x 10(-1) to 8.5 x 10(-1) per recipient. an improved method for the transformation of a. calcoaceticus bd413 is reported. | 1986 | 3941044 |
| antibacterial activity in vitro and regression studies for ceftazidime and ceftriaxone. | the antibacterial activity in vitro of ceftazidime and ceftriaxone was investigated against 575 recent clinical isolates. both cephalosporins displayed excellent activity against most of the pathogens tested, in particular gram-negative bacteria, including pseudomonas aeruginosa. apart from the pseudomonas group, acinetobacter calcoaceticus and campylobacter jejuni ceftriaxone was slightly to moderately more active than ceftazidime overall. ceftriaxone was moderately active against streptococcus ... | 1985 | 3929558 |
| energy transduction by electron transfer via a pyrrolo-quinoline quinone-dependent glucose dehydrogenase in escherichia coli, pseudomonas aeruginosa, and acinetobacter calcoaceticus (var. lwoffi). | the coupling of membrane-bound glucose dehydrogenase (ec 1.1.99.17) to the respiratory chain has been studied in whole cells, cell-free extracts, and membrane vesicles of gram-negative bacteria. several escherichia coli strains synthesized glucose dehydrogenase apoenzyme which could be activated by the prosthetic group pyrrolo-quinoline quinone. the synthesis of the glucose dehydrogenase apoenzyme was independent of the presence of glucose in the growth medium. membrane vesicles of e. coli, grow ... | 1985 | 3926746 |
| comparative in vitro activities of cefpiramide and apalcillin individually and in combination. | the in vitro activities of cefpiramide and apalcillin were compared with those of other third-generation cephalosporins and extended-spectrum penicillins against over 1,000 clinical bacterial isolates. the activity of cefpiramide against pseudomonas aeruginosa was comparable to those of piperacillin and cefoperazone, inhibiting 90% of strains at concentrations less than or equal to 16.0 micrograms/ml. this drug was also active against a broad range of gram-negative organisms but was generally le ... | 1985 | 3925875 |
| anti-pseudomonal activity of hr 810. | the anti-pseudomonal activity of hr 810, a new 2-aminothiazolyl cephalosporin, was compared to that of carbenicillin, azlocillin and cefsulodin against 187 non-fermenters. hr 810 was the best agent against pseudomonas aeruginosa, pseudomonas putida, pseudomonas fluorescens and acinetobacter calcoaceticus with an mic50 less than or equal to 4 mg/l and an mic90 less than or equal to 16 mg/l. it was as effective as azlocillin against pseudomonas stutzeri and pseudomonas mendocina, with an mic50 les ... | 1985 | 3922898 |
| solubilization of bacterial cells in organic solvents via reverse micelles. | a reverse micellar system containing tween 85 and water in isopropylpalmitate was developed which permitted the solubilization of bacteria in the form of homogenous organic solutions. the presence of the bacteria in solution was demonstrated by light microscopy. immediately after solubilization, isolated bacterial cells were observed, which by aging tend to form larger aggregates. cells of escherichia coli remained viable in this system for at least one day and retained beta-galactosidase activi ... | 1985 | 3921020 |
| membrane-bound lactate dehydrogenases and mandelate dehydrogenases of acinetobacter calcoaceticus. purification and properties. | procedures were developed for the optimal solubilization of d-lactate dehydrogenase, d-mandelate dehydrogenase, l-lactate dehydrogenase and l-mandelate dehydrogenase from wall + membrane fractions of acinetobacter calcoaceticus. d-lactate dehydrogenase and d-mandelate dehydrogenase were co-eluted on gel filtration, as were l-lactate dehydrogenase and l-mandelate dehydrogenase. all four enzymes could be separated by ion-exchange chromatography. d-lactate dehydrogenase and d-mandelate dehydrogenas ... | 1985 | 3904742 |
| [carbenicillin resistance of gram-negative bacteria: incidence, biochemical and genetic determinism]. | of nine hundred ampicillin resistant (amp-r) enterobacteria strains, isolated in hospital between july and december 1981, 73,7% are also carbenicillin-resistant (carb-r). this particular double resistance varies depending upon the species considered: indole positive proteus (23%), enterobacter cloacae (64%), citrobacter freundii (67%), acinetobacter calcoaceticus (73%), proteus mirabilis (75%), serratia marcescens (90%), escherichia coli (91%), providencia stuartii (96%) and klebsiella pneumonia ... | 1985 | 3903620 |
| [lack of specificity of protected distal bronchial samples in the bacteriological diagnosis of nosocomial pneumonia caused by gram negative bacilli]. | lower respiratory tract secretions (lrs) were sampled during a two year period in patients undergoing mechanical ventilation using a method previously described by matthew et al. only those lrs positive for gram-negative bacilli were reported in this study. the patients were separated in two groups: those with nosocomial pneumonia due to gram-negative bacilli (np+ group) and those without (np- group). the species most often isolated from lrs were: pseudomonas aeruginosa (25%), proteus (20%), esc ... | 1985 | 3893235 |
| membrane-bound lactate dehydrogenases and mandelate dehydrogenases of acinetobacter calcoaceticus. location and regulation of expression. | acinetobacter calcoaceticus possesses an l(+)-lactate dehydrogenase and a d(-)-lactate dehydrogenase. results of experiments in which enzyme activities were measured after growth of bacteria in different media indicated that the two enzymes were co-ordinately induced by either enantiomer of lactate but not by pyruvate, and repressed by succinate or l-glutamate. the two lactate dehydrogenases have very similar properties to l(+)-mandelate dehydrogenase and d(-)-mandelate dehydrogenase. all four e ... | 1985 | 3890833 |
| aztreonam in the treatment of serious orthopedic infections. | aztreozam was evaluated in the treatment of a variety of orthopedic infections. included were 17 patients with osteomyelitis, three with purulent arthropathy with prostheses, and 16 with superficial infections secondary to trauma or surgical procedure. pathogens were gram-negative bacilli sensitive to aztreonam. concomitant antibiotics were administered for gram-positive cocci that were present initially or by superinfection. infecting organisms included pseudomonas aeruginosa (minimal inhibitor ... | 1985 | 3881949 |
| plasmid transfer and behaviour in acinetobacter calcoaceticus ebf65/65. | at least one plasmid from each of the incompatibility groups b, c, fiv, h2/s, i alpha, i delta, p, w and x was shown to be capable of transfer from escherichia coli k12 to acinetobacter calcoaceticus ebf65/65. transfer was influenced by the presence of pav2 (thought to encode a restriction-modification system) in the recipient strain; however, not all plasmids belonging to a particular incompatibility group behaved identically. all plasmids were unstable to varying degrees in a. calcoaceticus eb ... | 1985 | 3851820 |
| evaluation of antibiotic susceptibility testing by agar dilution and the micro media system (fox panel). | the susceptibilities of 350 gram-positive cocci and 638 gram-negative bacilli to various antimicrobial agents were compared by using the micro-media system (mms) (fox panel) (micro-media systems, inc., potomac, md.) and a standard agar dilution procedure. major discrepancies occurred with enterococci, among which 48 of 53 isolates (91%) were found to be resistant to penicillin g by agar dilution and reported as susceptible by the mms. other large discrepancies occurred with staphylococcus aureus ... | 1985 | 3850093 |
| tolerance of acinetobacter calcoaceticus rag-1 to the cationic surfactant cetyltrimethylammonium bromide: role of the bioemulsifier emulsan. | emulsan, the polyanionic heteropolysaccharide bioemulsifier produced by acinetobacter calcoaceticus rag-1, was found to enhance the tolerance of rag-1 cells to the toxic effects of the cationic detergent cetyltrimethylammonium bromide (ctab). emulsan-mediated tolerance was obtained with the purified deproteinated apoemulsan; ca. 9 micrograms of apoemulsan neutralized 1 microgram of ctab. deesterified apoemulsan was only half as effective in protecting the cells from ctab toxicity. tolerance was ... | 1985 | 3838426 |
| exocellular esterase and emulsan release from the cell surface of acinetobacter calcoaceticus. | an esterase activity has been found, both in the cell-free growth medium and on the cell surface of the hydrocarbon-degrading acinetobacter calcoaceticus rag-1. the enzyme catalyzed the hydrolysis of acetyl and other acyl groups from triglycerides and aryl and alkyl esters. emulsan, the extracellular heteropolysaccharide bioemulsifier produced by strain rag-1, was also a substrate for the enzyme. gel filtration showed that the cell-free enzyme was released from the cell surface either emulsan fr ... | 1985 | 3838301 |
| studies on the chemical structure of the core-lipid a region of the lipopolysaccharide of acinetobacter calcoaceticus nctc 10305. detection of a new 2-octulosonic acid interlinking the core oligosaccharide and lipid a component. | lipopolysaccharide of acinetobacter calcoaceticus nctc 10305 was treated with acid (0.1 m hcl, 100 degrees c, 1 h). the product obtained (lpsdegr) was subjected to various modification and degradation procedures including reduction, hydrazinolysis and strong acid hydrolysis. methylation analysis of purified part structures revealed the presence of a 4'-phosphorylated (beta 1'-6)-linked d-glucosamine disaccharide (lipid a backbone), which carried in position 6' a hitherto unknown 2-octulosonic ac ... | 1987 | 3830168 |
| alcohol oxidation by acinetobacter calcoaceticus eb 104--a n-alkane-utilizing and cytochrome p-450-producing strain. | a soluble, nadp+-dependent alcohol dehydrogenase (adh) with a ph optimum at 8.7 was found in a. calcoaceticus eb 104 after growth on different carbon sources. n-alkanols with short and medium chain length were employed as test substrates. the km values decreased with increasing chain length. the vmax values remained nearly unchanged. the activities determined were independent of the carbon source. furthermore, a n-alkanol-dependent reduction of dcpip was measured in membrane fractions of cells g ... | 1986 | 3806370 |
| dienelactone hydrolase from pseudomonas sp. strain b13. | dienelactone hydrolase (ec 3.1.1.45) catalyzes the conversion of cis- or trans-4-carboxymethylenebut-2-en-4-olide (dienelactone) to maleylacetate. an approximately 24-fold purification from extracts of 3-chlorobenzoate-grown pseudomonas sp. strain b13 yielded a homogeneous preparation of the enzyme. the purified enzyme crystallized readily and proved to be a monomer with a molecular weight of about 30,000. each dienelactone hydrolase molecule contains two cysteinyl side chains. one of these was ... | 1987 | 3804973 |
| purification and characterization of quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus l.m.d. 79.41. | quinoprotein glucose dehydrogenase (ec 1.1.99.17) from acinetobacter calcoaceticus l.m.d. 79.41 was purified to homogeneity. it is a basic protein with an isoelectric point of 9.5 and an mr of 94,000. denaturation yields two molecules of pqq/molecule and a protein with an mr of 48000, indicating that the enzyme consists of two subunits, which are probably identical because even numbers of aromatic amino acids were found. the oxidized enzyme form has an absorption maximum at 350 nm, and the reduc ... | 1986 | 3800975 |
| in vitro susceptibility of acinetobacter species to various antimicrobial agents. | the in vitro activity of 18 antimicrobial agents against 40 clinical isolates each of acinetobacter calcoaceticus subsp. anitratum and acinetobacter lwoffi was studied. most of the newer 4-quinolone derivatives were extremely active against these organisms. newer beta-lactam agents, such as cefpirome, bmy 28142, and brl 36650, were also extremely active, inhibiting all strains at clinically achievable levels. most agents were two- to fourfold more active against a. lwoffi. | 1986 | 3800353 |
| isolation of acinetobacter calcoaceticus from cows with metritis. | 1986 | 3799102 | |
| prophage induction by ultraviolet light in acinetobacter calcoaceticus. | uv-induction of prophage p78 of acinetobacter calcoaceticus increased with the uv-dose given to the lysogenic strain from the spontaneous induction frequency of about 0.8% to a maximal frequency of 10%. this 10- to 20-fold increase of induction frequency, as measured by the number of infective centres, was accompanied by a 1000-fold increase in the yield of free phage. this effect was probably due to an increase in burst size under the conditions of lysogenic induction. unusually, the lysogen wa ... | 1986 | 3794658 |
| benzoate and muconate, structurally dissimilar metabolites, induce expression of cata in acinetobacter calcoaceticus. | biosynthetic regulation of cata, the gene encoding catechol 1,2-dioxygenase (ec 1.13.1.1), was studied in an acinetobacter calcoaceticus mutant strain unable to metabolize benzoate. benzoate and muconate independently induced the enzyme. in glucose-grown cells, benzoate yielded higher enzyme levels than did muconate, whereas muconate was the more effective inducer in succinate-grown cells. | 1987 | 3793718 |
| plasmid rp1-mediated susceptibility of acinetobacter calcoaceticus to rat polymorphonuclear leukocyte granule contents. | the resistance plasmid rp1 was transferred by conjugation to a plasmidless strain of acinetobacter calcoaceticus. acquisition and expression of rp1 by a. calcoaceticus ho1-n was associated with an increase in sensitivity to the antimicrobial activity of extracted contents from rat polymorphonuclear leukocyte granules. plasmid rp1-associated antibiotic resistance and sensitivity to granule contents were cured by exposure to acridine orange. assays with granule extract fractions separated by fast ... | 1986 | 3781624 |