Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| in vitro and in vivo antibacterial activities of fk037, a new parenteral cephalosporin. | in vitro and in vivo antibacterial activities of fk037, a new parenteral cephalosporin, were compared with those of cefpirome, ceftazidime and flomoxef. the advantages of in vitro activity of fk037 were as follows: (1) a broad-spectrum antibacterial activity, (2) the most potent activity (mic90: 25 micrograms/ml) of the cephalosporins tested against highly methicillin-resistant staphylococcus aureus (h-mrsa), (3) a strong activity against enterobacter spp. and citrobacter freundii resistant to t ... | 1994 | 8205935 |
| [hemagglutinating properties of acinetobacter calcoaceticus]. | hemagglutinating activity was tested in 309 strains of acinetobacter calcoaceticus with fresh and tannine human, horse, sheep, bovine, chicken and guinea pig erythrocytes. determinations were performed with 0.1% d-mannose and without it, both in temperature 37 and 22 degrees c. hemagglutinating activity was exhibited by 75-85% of strains, more frequently cultured at 22 degrees c a. calcoaceticus var. anitratus equally frequently was agglutinating both types of erythrocytes. strains of a calcoace ... | 1993 | 8189805 |
| acquisition of apparent dna slippage structures during extensive evolutionary divergence of pcad and catd genes encoding identical catalytic activities in acinetobacter calcoaceticus. | the pca operon from the gram- bacterium acinetobacter calcoaceticus encodes all of the enzymes required for catabolism of protocatechuate to common intermediary metabolites. this report presents the 2754-nucleotide (nt) sequence of a hindiii restriction fragment containing pcad, the 801-bp gene encoding beta-ketoadipate enol-lactone hydrolase i. the deduced primary structure of a. calcoaceticus pcad shares 44% amino acid (aa) sequence identity with the aligned primary structure of catd (beta-ket ... | 1994 | 8181753 |
| udp-n-acetylglucosamine 1-carboxyvinyl-transferase from acinetobacter calcoaceticus. | we have analyzed the sequence downstream of rpon from acinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to udp-n-acetylglucosamine 1-carboxyvinyl-transferase (murz). multicopy plasmids encoding this enzyme conferred phosphomycin resistance to a. calcoaceticus. the polar effect of a rpon mutation on the phosphomycin resistance level suggests that murz is, in part, cotranscribed with rpon. these observations confirm that a. calcoaceticus repre ... | 1994 | 8181716 |
| combined therapy of granulocyte transfusions, intravenous opsonins and antibiotics for gram-negative pneumonia in neutropenic cancer patients. | four patients with hematological malignancies, following bone marrow transplantation, who developed documented gram-negative [klebsiella pneumoniae (2), pseudomonas aeruginosa or acinetobacter calcoaceticus] pneumonia during absolute neutropenia, were treated with a combination of antimicrobial therapy, granulocyte transfusions, and high-dose intravenous immunoglobulin. the patients recovered following this regimen, including 2 who had septic shock and respiratory failure, necessitating intubati ... | 1994 | 8171936 |
| nadp-dependent alcohol dehydrogenases in bacteria and yeast: purification and partial characterization of the enzymes from acinetobacter calcoaceticus and saccharomyces cerevisiae. | an nadp-dependent constitutive alcohol dehydrogenase that can oxidize hexan-1-ol was detected in several gram-positive and gram-negative eubacteria and in two yeasts. the enzyme was purified to homogeneity from acinetobacter calcoaceticus ncib 8250 and from saccharomyces cerevisiae d273-10b. the bacterial enzyme appears to be a tetramer of subunit m(r) 40,300 and the yeast enzyme appears to be a monomer of subunit m(r) 43,500. the n-terminal amino acid sequence of the bacterial enzyme has 34% id ... | 1994 | 8162187 |
| azide-resistant mutants in acinetobacter calcoaceticus a2 are defective in protein secretion. | azide, an inhibitor of atpase, and a specific inhibitor of protein export was used in order to select for protein secretion mutants in acinetobacter calcoaceticus a2. two such mutants were isolated that were azide-resistant and defective in the general protein transport system. the mutation also conferred additional phenotypic changes, including an inability to grow on minimal media or at 40 degrees c. the existence of protein secretion mutants with a selectable phenotype may be useful for the g ... | 1994 | 8150267 |
| isolation, phenotypic characterization, and complementation analysis of mutants of methylobacterium extorquens am1 unable to synthesize pyrroloquinoline quinone and sequences of pqqd, pqqg, and pqqc. | aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (pqq) as a prosthetic group. seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with pqq have been isolated in the facultative methanol utilizer methylobacterium extorquens am1. in addition, 12 previously isolated methanol oxidation mutants of m. extorquens am1 were shown to be able to grow on methanol in the prese ... | 1994 | 8132470 |
| unusual g + c content and codon usage in catijf, a segment of the ben-cat supra-operonic cluster in the acinetobacter calcoaceticus chromosome. | the nucleotide (nt) sequence of a 5.3-kb dna segment containing the gram- acinetobacter calcoaceticus catbcijfd operon is reported. this information completes determination of a 16-kb nt sequence containing the twelve ben and cat structural genes encoding enzymes required for catabolism of benzoate via the beta-ketoadipate pathway. many of these genes can be traced to a common ancestry with genes from other organisms containing dna with widely divergent g + c content. the a. calcoaceticus ben an ... | 1994 | 8125318 |
| community-acquired bacteraemic acinetobacter pneumonia with survival. | a 65 year old man was admitted with segmental consolidation of the left upper lobe after having stayed in a hotel for 2 days. he deteriorated rapidly on conventional antibiotic therapy and required ventilatory support. acinetobacter calcoaceticus var. anitratus was grown from the sputum and blood cultures, which was treated with a combination of anti-pseudomonal agent, aminoglycoside and cotrimoxazole. he made a slow but remarkable recovery from the pneumonia. acinetobacter is a rare potentially ... | 1993 | 8121870 |
| crystallization and preliminary x-ray analysis of protocatechuate 3,4-dioxygenase from acinetobacter calcoaceticus. | x-ray quality single crystals of protocatechuate 3,4-dioxygenase from acinetobacter calcoaceticus were obtained by the hanging drop method. the intradiol dioxygenase crystallizes in the cubic space group i23 with unit cell dimensions a = b = c = 145.5 a. the dodecahedral crystals diffract to beyond 2.5 a resolution. the asymmetric unit contains one twelfth of the enzyme (alpha beta fe+3)12 complex. | 1994 | 8107118 |
| comparison of three typing methods in hospital outbreaks of acinetobacter calcoaceticus infection. | during a period of 11 months acinetobacter baumanii was isolated from 27 patients and 21 environmental samples in the intensive care unit (icu) and in one surgical unit of a university hospital. the isolates were characterized by biotyping, antibiograms and plasmid profiles and compared with co-isolates. plasmid fingerprinting distinguished three outbreaks, whereas other typing methods were less sensitive and discriminatory. although plasmid profiles seem to be a simple and reproducible marker f ... | 1993 | 8097217 |
| contrasting patterns of evolutionary divergence within the acinetobacter calcoaceticus pca operon. | the six enzymes required for catabolism of protocatechuate to succinate and acetylcoa are encoded by the pca genes in the gram-bacterium, acinetobacter calcoaceticus. the clustered a. calcoaceticus cat genes encode an analogous set of enzymes associated with the metabolic dissimilation of catechol. the nucleotide (nt) sequences of pcaijfb and pcak, reported here, complete evidence showing that all of the pca structural genes are tightly grouped in the order pcaijfbdkchg within a single operon. t ... | 1994 | 8063101 |
| regulation of spvr gene expression of salmonella virulence plasmid pkdsc50 in salmonella choleraesuis serovar choleraesuis. | the expression regulation of spvr, a regulatory gene on the virulence plasmid (pkdsc50) of salmonella choleraesuis serovar choleraesuis, was investigated by spvr-lacz translational fusion. the spvr gene was found to be positively regulated by its own product, the spvr protein, and this unusual positive autoregulation was repressed by the products of spva and spvb, virulence-associated genes present downstream from the spvr gene. amino acid sequence analysis revealed that the amino-terminal regio ... | 1994 | 8052129 |
| comparative serum bactericidal activities of three doses of ciprofloxacin administered intravenously. | the pharmacokinetics and serum bactericidal activities of three intravenous doses of ciprofloxacin were studied comparatively in 30 patients. single 200-, 300-, and 400-mg intravenous doses of ciprofloxacin were given over 30 min to 10 patients each, and serum samples were obtained at 0.5, 1, 2, 3, 4, 8, and 12 h after the start of the infusion. serum drug concentrations were determined by high-pressure liquid chromatography. pharmacokinetic parameters were estimated by using noncompartmental an ... | 1994 | 8031055 |
| inability of muconate cycloisomerases to cause dehalogenation during conversion of 2-chloro-cis,cis-muconate. | the conversion of 2-chloro-cis,cis-muconate by muconate cycloisomerase from pseudomonas putida prs2000 yielded two products which by nuclear magnetic resonance spectroscopy were identified as 2-chloro- and 5-chloromuconolactone. high-pressure liquid chromatography analyses showed the same compounds to be formed also by muconate cycloisomerases from acinetobacter calcoaceticus adp1 and pseudomonas sp. strain b13. during 2-chloro-cis,cis-muconate turnover by the enzyme from p. putida, 2-chloromuco ... | 1994 | 8021223 |
| regulation of p-hydroxybenzoate hydroxylase synthesis by pobr bound to an operator in acinetobacter calcoaceticus. | pobr is a transcriptional activator required for the expression of poba, the structural gene for p-hydroxybenzoate hydroxylase. the poba and pobr genes are divergently transcribed and separated by 134 bp in the acinetobacter calcoaceticus chromosome. primer extension analysis revealed that the poba transcript begins 22 bp upstream from the structural gene and the pobr transcript begins 69 bp upstream from the regulatory gene. this arrangement requires superimposition of the -10 base pair and -35 ... | 1994 | 8021213 |
| the pca-pob supraoperonic cluster of acinetobacter calcoaceticus contains quia, the structural gene for quinate-shikimate dehydrogenase. | an 18-kbp acinetobacter calcoaceticus chromosomal segment contains the pcaijfbdkchg operon, which is required for catabolism of protocatechuate, and pobsra, genes associated with conversion of p-hydroxybenzoate to protocatechuate. the genetic function of the 6.5 kbp of dna between pcag and pobs was unknown. deletions in this dna were designed by removal of fragments between restriction sites, and the deletion mutations were introduced into a. calcoaceticus by natural transformation. the mutation ... | 1994 | 8002591 |
| bactericidal activity of cefpirome (hr 810) against 513 gram-negative bacteria isolated from blood of septicemic patients. | the profile of antibacterial activity of cefpirome was compared with that of nine other antimicrobial agents against 513 gram-negative bacteria isolated from septicemic patients. all strains were evaluated for their sensitivity by disc diffusion and broth dilution tests (mic and mbc). cefpirome was compared to cefazolin, cefuroxime, ceftazidime, ceftriaxone, aztreonam, imipenem, ticarcillin, tobramycin and ciprofloxacin. among the five cephalosporins tested in this study, cefpirome was the most ... | 1994 | 8002094 |
| l-carnitine via enzyme-catalyzed oxidative kinetic resolution. | l-carnitine of high optical purity was prepared via kinetic resolution using a mutant strain of acinetobacter calcoaceticus atcc 39647. this organism preferentially metabolized the d-enantiomer of the racemate to furnish l-carnitine. recovery of l-carnitine was 93%, providing a total weight yield of 46.5% in 92% enantiomeric excess. the mode of degradation of carnitine was shown to proceed via a monooxygenase-catalyzed oxidative cleavage resulting in the formation of trimethylamine and malic aci ... | 1994 | 8000862 |
| [clinical evaluation of sulbactam/cefoperazone for infections during the chemotherapy of hematologic malignancy]. | in 28 patients with hematologic malignancy, the clinical effect and the safety of sulbactam/cefoperazone (sbt/cpz) were studied on 45 episodes during granulocytopenic stage after antineoplastic chemotherapy. the overall efficacy rate obtained with sbt/cpz in combination with other drugs was 62%. the rates for sepsis, suspected sepsis and pneumonia were 67%, 94% and 38%, respectively. the efficacy in those cases that showed granulocyte count less than 300/microliters during the course of administ ... | 1994 | 7990260 |
| [adhesion of acinetobacter calcoaceticus to cheek epithelial cells]. | adhesive properties of 309 strains of acinetobacter calcoaceticus were determined toward cells of cheek epithelium obtained from 13 healthy women. most of strains adhered in number of > 0-20 bacterial cells for one epithelial cell. adherence of a. calcoaceticus to cheek epithelium was dependent not only of variety, strain and temperature of bacterial culture, but also of individual properties of epithelial cells obtained from donors. relation between hydrophobic properties and adherence to cheek ... | 1994 | 7967922 |
| [hydrophobic properties of acinetobacter calcoaceticus]. | evaluation of hydrophobic properties of 309 strains of acinetobacter calcoaceticus was performed by a sat method. among investigated strains cultured at 22 degrees c, 103 strains were autoaggregating, representing very strong hydrophobic properties. most of strains aggregated at concentration of 0.4-1.0 m (nh4)2so4, exhibiting strong hydrophobic activities. only 38 strains during culture at 22 degrees c exhibited hydrophilic surface properties. differences between strains of anitratus variety an ... | 1994 | 7967921 |
| purification and properties of a novel type of malonate decarboxylase from acinetobacter calcoaceticus. | a novel type of malonate decarboxylase which is induced and located in periplasm of acinetobacter calcoaceticus grown on malonate as sole carbon and energy source was purified to homogeneity. the purified 185-kda decarboxylase was found to be an oligomer composed of three different polypeptides in a ratio of 2:1:1, designated alpha (65 kda), beta (32 kda), and gamma (25 kda). optimum ph was 6.8, while pi was 6.39. the enzyme was highly specific for malonate with km 1.4 mm. this enzyme contained ... | 1994 | 7961952 |
| inducible cell lysis system for the study of natural transformation and environmental fate of dna released by cell death. | two novel conditional broad-host-range cell lysis systems have been developed for the study of natural transformation in bacteria and the environmental fate of dna released by cell death. plasmid pdkl02 consists of lysis genes s, r, and rz from bacteriophage lambda under the control of the ptac promoter. the addition of inducer to escherichia coli, acinetobacter calcoaceticus, or pseudomonas stutzeri containing plasmid pdkl02 resulted in cell lysis coincident with the release of high amounts of ... | 1994 | 7961508 |
| a gene of acinetobacter calcoaceticus bd413 encodes a periplasmic peptidyl-prolyl cis-trans isomerase of the cyclophilin sub-class that is not essential for growth. | downstream of the acinetobacter calcoaceticus esta gene, encoding a cell-bound esterase, an open reading frame (orf) was identified, which may encode a protein with a mass of 20.4 kda. this protein shows extensive similarity to both prokaryotic and eukaryotic peptidyl-prolyl cis-trans isomerases (ppiases) of the cyclophilin sub-class, especially to the periplasmic rotamase (rota) of escherichia coli. a putative signal sequence suggests that the product of the acinetobacter gene, we termed rota, ... | 1994 | 7948017 |
| [degradation of oil derivatives by acinetobacter calcoaceticus mm5]. | this paper describes the isolation of microorganisms from polluted heating oil. the growth of one of them has been studied (acinetobacter calcoaceticus mm5) in several linear and branched hydrocarbons as well as the effect of its growth on commercial diesel oil. acinetobacter calcoaceticus mm5 is not capable of using glucose as its only source of carbon, and it needs the presence of nitrogen and phosphorus sources to degrade any petroleum by-product. | 1994 | 7946119 |
| community-acquired bacteremias from tunneled central intravenous lines: results from studies of a single vendor. | tunneled central intravenous catheters are a common method for rendering prolonged outpatient intravenous therapy. their safety, however, has not been well studied. we conducted a retrospective evaluation of bacteremias associated with tunneled central intravenous catheters managed by a single home health care vendor during a 1-year period. all catheters were inserted in the operating room under sterile conditions. to calculate total line days, the dates of catheter insertion and removal were ob ... | 1994 | 7943925 |
| properties of acinetobacter calcoaceticus reca and its contribution to intracellular gene conversion. | the acinetobacter calcoaceticus pcaj and catj genes, nearly identical in dna sequence, differ in transcriptional control and are separated by more than 20 kb of chromosomal dna. the pcaj3125 mutation is repaired frequently in organisms containing the wild-type catj gene. this high-frequency repair is eliminated in strains lacking the catj gene, which suggests that recombination between the homologous catj and pcaj genes contributes to the high-frequency repair of the pcaj3125 mutation. we report ... | 1994 | 7934905 |
| [laboratory and clinical studies on combined effects of fosfomycin plus sulbactam/cefoperazone for mixed infections of mrsa and pseudomonas aeruginosa]. | the time-lag/sequential/step by step chemotherapy by fosfomycin plus sulbactam/cefoperazone including a short time small dose of steroid was done for 27 severely infected patients suffered from various cancer or other severe basal diseases. and the staggered "intensive" chemotherapy with added arbekacin or vancomycin to the previous staggered chemotherapy was done against 13 severely infected patients having mrsas among their infecting pathogens. 1. bacteriological effects pseudomonas aeruginosa ... | 1994 | 7933542 |
| [susceptibilities of glucose non-fermentative gram-negative bacilli to antibiotics]. | glucose non-fermentative gram-negative bacilli are important nosocomial pathogens. this study concerned with susceptibilities to antibacterial agents of strains of glucose non-fermentative gram-negative bacilli that were isolated from cultures of clinical materials at 123 hospital laboratories throughout japan from september to december of 1991. the tests for susceptibilities were performed according to the disk dilution method recommended by nccls. the following bacteria were tested: pseudomona ... | 1994 | 7933532 |
| evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates. | the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ... | 1994 | 7933531 |
| analysis of the interaction between autochthonous bacteria and packaging material in pvc-bottled mineral water. | a study with about 10,000 bottles produced by a mineral water company was undertaken in order to identify the causal agent of an off-odour occurrence in the bottled water. some physiological attributes of the dominant species over an 8-month period, as well as their interaction with packaging material, were investigated. pseudomonas maltophilia, p. acidovorans, acinetobacter calcoaceticus var. lowffi, frequently associated with bottles having an off-odour, seemed to play a decisive role in the p ... | 1994 | 7921893 |
| regulation of the hema gene during 5-aminolevulinic acid formation in pseudomonas aeruginosa. | the general tetrapyrrole precursor 5-aminolevulinic acid is formed in bacteria via two different biosynthetic pathways. members of the alpha group of the proteobacteria use 5-aminolevulinic acid synthase for the condensation of succinyl-coenzyme a and glycine, while other bacteria utilize a two-step pathway from aminoacylated trna(glu). the trna-dependent pathway, involving the enzymes glutamyl-trna reductase (encoded by hema) and glutamate-1-semialdehyde-2,1-aminomutase (encoded by heml), was d ... | 1995 | 7883699 |
| nucleotide sequences transferred by gene conversion in the bacterium acinetobacter calcoaceticus. | exchange of nucleotide (nt) sequences between the catijf and pcaljf regions of the acinetobacter calcoaceticus chromosome appears to contribute to frequent repair of mutations, including removal of a tn5 insertion from pcaj. repaired nt sequences are the products of nonreciprocal genetic exchange. the length of donor catijf nt tracts recovered in repaired pcaijf dna ranged from less than 315 nt to more than 881 nt. this evidence does not distinguish gene conversion from natural transformation as ... | 1995 | 7883173 |
| degradation of propanil by bacterial isolates and mixed populations from a pristine lake. | the microbial transformation rates of propanil, a commonly used herbicide, were investigated using water from a pristine lake in northeast georgia. microbial degradation rates were measured using natural water microflora, the natural water microflora amended with five bacterial species (aerobacter aerogenes, aeromonas hydrophila, acinetobacter calcoaceticus, proteus mirabilis, and aeromonas salmonicida) isolated from the same lake, and the five isolates individually. transformation rate constant ... | 1995 | 7874462 |
| spontaneous mutations in pcah and -g, structural genes for protocatechuate 3,4-dioxygenase in acinetobacter calcoaceticus. | bacteria containing spontaneous null mutations in pcah and -g, structural genes for protocatechuate 3,4-dioxygenase, were selected by exposure of an acinetobacter calcoaceticus strain to physiological conditions in which expression of the genes prevents growth. the parental bacterial strain exhibits high competence for natural transformation, and this procedure was used to characterize 94 independently isolated spontaneous mutations. four of the mutations were caused by integration of a newly id ... | 1995 | 7868609 |
| molecular typing of acinetobacter baumannii-acinetobacter calcoaceticus complex isolates from endemic and epidemic nosocomial infections. | ribotype, biotype and resistance phenotype were used to characterize 37 acinetobacter baumannii-a. calcoaceticus complex isolates responsible for nosocomial infections in buenos aires. nineteen isolates were recovered from endemic infections at 2 hospitals and 18 represent an intensive care unit outbreak that occurred in a third hospital. by ribotyping isolates were classified into five different clones of a. baumannii biotype 2, 3 of a. baumannii biotype 9, and 3 of acinetobacter genospecies 13 ... | 1995 | 7867730 |
| microbial baeyer-villiger reaction of bicyclo[3.2.0]heptan-6-ones--a novel approach to sarkomycin a. | racemic (1 alpha, 2 alpha, 5 alpha)- and (1 beta, 2 alpha, 5 beta)-2- bromobicyclo[3.2.0]heptan-6-one (rac-7, rac-10, respectively), (1 alpha, 2 alpha, 5 beta)- and (1 beta, 2 alpha, 5 beta)-2- benzyloxybicyclo[3.2.0]heptan-6-one (rac-15, rac-13, respectively), (1 beta, 2 alpha, 5 beta)-2-hydroxybicyclo[3.2.0]heptan-6-one (rac-17) and cis-bicyclo[3.2.0]hept-2-en-7-one (rac-18) were subjected to a microbial baeyer-villiger reaction by acinetobacter calcoaceticus ncib 9871. in each case both regio ... | 1994 | 7858964 |
| acinetobacter calcoaceticus anitratus outbreak in the intensive care unit traced to a peak flow meter. | a cluster of seven cases of acinetobacter caleoaceticus anitratus in a community teaching hospital intensive care unit was discovered (the seventh case was located in a step-down unit next to an infected patient recently transferred from the intensive care unit.) | 1994 | 7847640 |
| in-vitro activity of ampicillin/sulbactam against multiresistant acinetobacter calcoaceticus var. anitratus clinical isolates. | 1994 | 7814296 | |
| in vitro antibacterial activity and beta-lactamase stability of sy5555, a new oral penem antibiotic. | the antibacterial activity of sy5555, a new oral penem antibiotic, was compared with those of cefaclor, cefixime, and cefteram. sy5555 was more active than the comparison agents against methicillin-susceptible staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, enterococcus faecalis, citrobacter freundii, enterobacter cloacae, morganella morganii, acinetobacter calcoaceticus, clostridium spp., and bacteroides fragilis. against providencia spp., proteus spp., and haemophi ... | 1994 | 7811005 |
| characterization of the chromosomal aac(6')-ij gene of acinetobacter sp. 13 and the aac(6')-ih plasmid gene of acinetobacter baumannii. | the amikacin resistance genes aac(6')-ih of acinetobacter baumannii bm2686 and aac(6')-ij of acinetobacter sp. 13 bm2689 encoding aminoglycoside 6'-n-acetyltransferases were characterized. the 441-bp coding sequences predict proteins with calculated masses of 16,698 and 16,677 da, respectively. analysis of the deduced amino acid sequences indicated that the proteins belonged to a subfamily of 6'-aminoglycoside acetyltransferase type i enzymes from gram-negative bacteria. the aac(6')-ih gene of b ... | 1994 | 7810994 |
| elucidation of the region responsible for edta tolerance in pqq glucose dehydrogenases by constructing escherichia coli and acinetobacter calcoaceticus chimeric enzymes. | we constructed various chimeric pqq glucose dehydrogenases (pqqgdhs) from an edta-sensitive pqqgdh from escherichia coli and an edta-tolerant pqqgdh from acinetobacter calcoaceticus by homologous recombination of their structural genes. the edta tolerance of the resulting chimeric enzymes was investigated. our results demonstrated that edta tolerance of pqqgdhs can be completely altered by substituting each corresponding region. the edta tolerance of a. calcoaceticus pqqgdh is mostly within a re ... | 1995 | 7779095 |
| characterization of lipase-deficient mutants of acinetobacter calcoaceticus bd413: identification of a periplasmic lipase chaperone essential for the production of extracellular lipase. | acinetobacter calcoaceticus bd413 produces an extracellular lipase, which is encoded by the lipa gene. five lipase-deficient mutants have been generated via random insertion mutagenesis. phenotypic characterization of these mutants revealed the presence of as many as four lipolytic enzymes in a. calcoaceticus. biochemical evidence classified four of the mutants as export mutants, which presumably are defective in translocation of the lipase across the outer membrane. the additional mutant, desig ... | 1995 | 7768830 |
| the glucose dehydrogenase-mediated energization of acinetobacter calcoaceticus as a tool for evaluating its susceptibility to, and defence against, hazardous chemicals. | cells of acinetobacter calcoaceticus 69-v could be energized by glucose oxidation after the growth on acetate, ethanol, hexanol and benzoate. the velocities of glucose oxidation-driven atp syntheses were relatively constant in the range from ph 5.4 to 7.5. with decreasing ph values (7.0, 6.0, 5.4) atp synthesis was inhibited more strongly by the action of 2,4-dinitrophenol and at the same ph value glucose oxidation was nearly unimpaired or inhibited more weakly. this finding is expressed by a de ... | 1995 | 7765916 |
| acinetobacter calcoaceticus liberates chromosomal dna during induction of competence by cell lysis. | a transformation assay was used to assay the amount of dna present in the extracellular medium of a growing culture of acinetobacter calcoaceticus. it was observed that small amounts of dna were liberated during the entire exponential growth phase in a batch culture. release of dna could be fully accounted for by lysis of cells. lysis was quantified via simultaneous measurement of beta-galactosidase activity of cells and supernatant, with a strain that contained a plasmid (papa100) with lacz und ... | 1995 | 7765885 |
| thermostable chimeric pqq glucose dehydrogenase. | the thermal stability of pqq glucose dehydrogenases (pqqgdhs) which were chimeras with more than 95% made up of the n-terminal region of escherichia coli pqqgdh and the rest made up of the c-terminal region of acinetobacter calcoaceticus pqqgdh was investigated. among the chimeric pqqgdhs, e97a3 (e. coli 97% and a. calcoaceticus 3%) and e95a5 were found to possess higher thermal stability than parental e. coli pqqgdh. further detailed characterization of the thermal stability was carried out, fo ... | 1995 | 7758590 |
| different relative importances of the par operons and the effect of conjugal transfer on the maintenance of intact promiscuous plasmid rk2. | the par region of the broad-host-range, incp alpha plasmid rk2 has been implicated as a stability determinant by its ability to enhance the maintenance of mini-rk2 plasmids or heterologous replicons in a growing population of host cells. the region consists of two operons: parcba, which encodes a multimer resolution system, and parde, which specifies a postsegregational response mechanism that is toxic to plasmidless segregants. to assess the importance of this region to the stable maintenance o ... | 1995 | 7751288 |
| a periplasmic insulin-cleaving proteinase (icp) from acinetobacter calcoaceticus sharing properties with protease iii from escherichia coli and ide from eucaryotes. | a periplasmic insulin-cleaving proteinase (icp), purified to its electrophoretic homogeneity in the sds-page from the gram-negative bacterium acinetobacter calcoaceticus, was examined and compared in its properties with the protease iii (protease pi, pitrilysin, ec 3.4.99.44) of escherichia coli and the insulin-destroying proteinase (ide, insulinase, ec 3.4.99.45) from eucaryotes. the enzyme was proven to be a metalloprotease like protease iii and ide, as was shown by the inhibitory effects exer ... | 1995 | 7738784 |
| nk-lysin, a novel effector peptide of cytotoxic t and nk cells. structure and cdna cloning of the porcine form, induction by interleukin 2, antibacterial and antitumour activity. | a 78 residue antimicrobial, basic peptide, nk-lysin, with three intrachain disulfide bonds was purified from pig small intestine and characterized. a corresponding clone was isolated from a porcine bone marrow cdna library. the 780 bp dna sequence had a reading frame of 129 amino acids which corresponded to nk-lysin. the clone was used to show that stimulation with human interleukin-2 induced synthesis of nk-lysin-specific mrna in a lymphocyte fraction enriched for t and nk cells. lower levels o ... | 1995 | 7737114 |
| two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in acinetobacter calcoaceticus adp1. | mutants of acinetobacter calcoaceticus adp1 unable to grow on dodecane, but retaining the ability to grow on lauric acid were isolated after ethylmethanesulphonate (ems) treatment. this growth deficiency was complemented by a clone from a gene library constructed from chromosomal dna of the wild-type strain. the complementing dna mapped in a gene encoding a polypeptide with homology to rubredoxins. the deduced putative rubredoxin amino acid sequence is more similar to related proteins from gram- ... | 1995 | 7670642 |
| phosphate uptake by immobilized acinetobacter calcoaceticus cells in a full scale activated sludge plant. | an in situ study of the p-uptake ability of acinetobacter calcoaceticus was carried out using the alginate immobilization technique. immobilized a. calcoaceticus cells displayed a high p-uptake ability (> 97% p-accumulating cells) when immersed in the aerobic zone of an activated sludge system for 30-240 min. the overall p-accumulation pattern of the anaerobic zone depicted a typical p-release mechanism. however, limited p-accumulation was also observed at this stage. growth and anaerobiosis wer ... | 1995 | 7662295 |
| a case of acinetobacter calcoaceticus pneumonia. | a case of community acquired pneumonia with acinetobacter calcoaceticus is presented. acinetobacter must be considered in the differential diagnosis of gram negative coccobacillary pneumonia. | 1995 | 7660350 |
| genomic species typing of acinetobacters by polymerase chain reaction amplification of the reca gene. | the reca gene has been used as a target in screening for the presence of acinetobacters on the genospecies level and differentiation of relevant acinetobacter species from one another by pcr. primers deduced from known reca gene sequences of acinetobacter calcoaceticus and neisseria gonorrhoeae allowed the amplification of dnas from all acinetobacter genospecies. the size of the amplified dna fragment from all genospecies tested was approximately 435-500 bp relative to dna size markers. the ampl ... | 1995 | 7649456 |
| purification and characterization of 4-hydroxybenzoate 3-hydroxylase from a klebsiella pneumoniae mutant strain. | unlike the parent wild-type strain, the klebsiella pneumoniae mutant strain mao4 has a 4-hba+ phenotype. the capacity of this mutant to take up and metabolize 4-hydroxybenzoate (4-hba) relies on the expression of a permease and an nadph-linked monooxygenase (4-hba-3-hydroxylase). both enzymes are normally expressed at basal levels, and only the presence of 4-hba in the media enhances their activities. strikingly, when the acinetobacter calcoaceticus poba gene encoding 4-hydroxybenzoate-3-hydroxy ... | 1995 | 7646318 |
| novel bioemulsifiers from microorganisms for use in foods. | the main objective of this study was to test the range of microorganisms for production of extracellular, high molecular weight emulsifiers for potential use in foods. a standard emulsification assay developed specifically for assessing food emulsifiers was used to examine 24 extracellular microbial products from bacteria, yeasts and algae. of the 24 products tested, nine had emulsification ability that was as good as and eight had emulsifying properties that were better than those of the common ... | 1995 | 7632394 |
| a transposon-like sequence adjacent to the acci restriction-modification operon. | we have cloned and sequenced the accirm genes from weeksella zoohelcum (the original identification of this strain as acinetobacter calcoaceticus was incorrect). our sequence differs in the coding regions from a previously published sequence by the addition of three nucleotides near the 3' end of the dna methyltransferase-encoding gene (accim). we have sequenced approx. 3 kb beyond this operon. two genes were found, convergently transcribed with the r-m operon. the first of these genes encodes a ... | 1995 | 7607529 |
| [analysis of 1116 strains of pathogens isolated from infected burn wounds]. | we report the analysis of 1,116 strains of pathogens isolated from infected burn wounds of 536 patients hospitalized from 1989 to 1991. from the 1,116 strains of pathogens, 39 species of aerobes and fungi were found, including 217 strains of staphylococcus aureus, 208 strains of pseudomonas aeruginosa and 119 strains of acinetobacter calcoaceticus. the positive rates of the above three bacteria were 19.4%, 18.6% and 10.6% respectively. some opportunistic pathogens, such as bacillus cercus, aeroc ... | 1995 | 7600434 |
| degradation of trans-ferulic and p-coumaric acid by acinetobacter calcoaceticus dsm 586. | cell suspensions of acinetobacter calcoaceticus strain dsm 586 and dsm 590 were able to grow on benzoic, p-hydroxybenzoic and vanillic acid as sole carbon source. testing the utilization of trans-ferulic and p-coumaric acid, we found that the sole a. calcoaceticus dsm 586 efficiently degraded the lignocellulose related monomers. cells induced with trans-ferulic acid were able to oxidize trans-ferulic, p-coumaric, vanillic, p-hydroxybenzoic and protocatechuic acid at rates higher than the uninduc ... | 1995 | 7599157 |
| mixed meningitis: association of acinetobacter calcoaceticus var lwoffi and streptococcus faecium. | we describe mixed bacterial meningitis in a young man due to acinetobacter calcoaceticus var lwoffi and streptococcus faecium. the combination of a calcoaceticus var lwoffi and s faecium as aetiology of mixed bacterial meningitis has not been previously reported. the patient recovered completely without neurologic sequelae on chloramphenicol and penicillin. | 1995 | 7596936 |
| characterization of the extracellular lipase, lipa, of acinetobacter calcoaceticus bd413 and sequence analysis of the cloned structural gene. | the extracellular lipase from acinetobacter calcoaceticus bd413 was purified to homogeneity, via hydrophobic-interaction fast performance liquid chromatography (fplc), from cultures grown in mineral medium with hexadecane as the sole carbon source. the enzyme has an apparent molecular mass of 32 kda on sds-polyacrylamide gels and hydrolyses long acyl chain p-nitrophenol (pnp) esters, like pnp palmitate (pnpp), with optimal activity between ph 7.8 and 8.8. additionally, the enzyme shows activity ... | 1995 | 7596283 |
| distance between alleles as a determinant of linkage in natural transformation of acinetobacter calcoaceticus. | cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor acinetobacter calcoaceticus dna. a different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 kb. ligation of unlinked chromosomal dna fragments allowed them to be linked genetically through natural transformation. | 1995 | 7592360 |
| unusual ancestry of dehydratases associated with quinate catabolism in acinetobacter calcoaceticus. | catabolism of quinate to protocatechuate requires the consecutive action of quinate dehydrogenase (quia), dehydroquinate dehydratase (quib), and dehydroshikimate dehyratase (quic), genes for catabolism of protocatechuate are encoded by the pca operon in the acinetobacter calcoaceticus chromosome. observations reported here demonstrate that a. calcoaceticus qui genes are clustered in the order quibcxa directly downstream from the pca operon. sequence comparisons indicate that quix encodes a porin ... | 1995 | 7592351 |
| catm encodes a lysr-type transcriptional activator regulating catechol degradation in acinetobacter calcoaceticus. | on the basis of the constitutive phenotypes of two catm mutants of acinetobacter calcoaceticus, the catm protein was proposed to repress expression of two different loci involved in catechol degradation, cata and catbcijfd (e. neidle, c. hartnett, and l. n. ornston, j. bacteriol. 171:5410-5421, 1989). in spite of its proposed negative role as a repressor, catm is similar in amino acid sequence to positive transcriptional activators of the lysr family. investigating this anomaly, we found that in ... | 1995 | 7592340 |
| biosensor based on an enzyme modified electrode for highly-sensitive measurement of polyphenols. | the use of glucose dehydrogenase from acinetobacter calcoaceticus for highly sensitive measurement of polyphenols, based on bioelectrocatalytic analyte recycling, has been demonstrated. a polyphenol (analyte) is oxidized on the surface of a glassy carbon electrode at an anodic potential and is regenerated by immobilized glucose dehydrogenase (gdh) in the presence of glucose, resulting in an amplified response. the dynamic properties of the enzyme-modified glassy carbon electrode allow the conven ... | 1995 | 7576439 |
| the in-vitro activity of cp 99,219, a new naphthyridone antimicrobial agent: a comparison with fluoroquinolone agents. | the in-vitro activity of a new fluoroquinolone, cp 99,219 was compared with that of ciprofloxacin, du 6859, sparfloxacin and levofloxacin. cp 99,219 showed generally similar in-vitro activity to the other compounds tested against the enterobacteriaceae (mic90 of all agents < 0.5 mg/l except morganella morganii). it was found to be more active than ciprofloxacin, sparfloxacin and levofloxacin against the strains of acinetobacter calcoaceticus tested (mic90 1 mg/l). cp 99,219 was found to be four- ... | 1995 | 7559198 |
| comparison of ribotyping and pulsed-field gel electrophoresis for molecular typing of acinetobacter isolates. | seventy-three isolates of the acinetobacter calcoaceticus-acinetobacter baumannii complex, including 26 isolates from 10 hospital outbreaks, were typed by ribotyping with ecori and clai and by pulsed-field gel electrophoresis (pfge) of genomic dna after digestion with apai. ribotyping with ecori distinguished 31 ribopatterns. digestion with clai generated another eight ribotypes. pfge, in contrast, identified 49 distinct patterns with seven variants. both methods detected all outbreak-related is ... | 1995 | 7542274 |
| cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs. | the two-component nonheme iron dioxygenase system 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs catalyzes the double hydroxylation of 2-halobenzoates with concomitant release of halogenide and carbon dioxide, yielding catechol. the gene cluster encoding this enzyme, cbdabc, was localized on a 70-kbp conjugative plasmid designated pbah1. the nucleotide sequences of cbdabc and flanking regions were determined. in the deduced amino acid sequence of the large subunit of the terminal o ... | 1995 | 7530709 |
| nucleotide sequence and functional analysis of the genes encoding 2,4,5-trichlorophenoxyacetic acid oxygenase in pseudomonas cepacia ac1100. | pseudomonas cepacia ac1100 is able to use the chlorinated aromatic compound 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) as the sole source of carbon and energy. one of the early steps in this pathway is the conversion of 2,4,5-t to 2,4,5-trichlorophenol (2,4,5-tcp). 2,4,5-tcp accumulates in the culture medium when ac1100 is grown in the presence of 2,4,5-t. a dna region from the ac1100 genome has been subcloned as a 2.7-kb ssti-xbai dna fragment, which on transfer to pseudomonas aeruginosa pao1 ... | 1994 | 7527626 |
| pcr-based preparation of 23s rrna-targeted group-specific polynucleotide probes. | dna coding for a variable region within domain iii of bacterial 23s rrna was used as the target for group-specific polynucleotide hybridization probes. the corresponding rdna was amplified in vitro by the pcr technique in combination with a pair of primers specific for flanking conserved target sites. the amplified fragments were cloned or used directly as probes. rna probes were generated by in vitro transcription of cloned or amplified rdna. the probes were labeled by incorporating modified nu ... | 1994 | 7524442 |
| phylogenetic relationships between some members of the genera neisseria, acinetobacter, moraxella, and kingella based on partial 16s ribosomal dna sequence analysis. | we obtained 16s ribosomal dna (rdna) sequence data for strains belonging to 11 species of proteobacteria, including the type strains of kingella kingae, neisseria lactamica, neisseria meningitidis, moraxella lacunata subsp. lacunata, [neisseria] ovis, moraxella catarrhalis, moraxella osloensis, [moraxella] phenylpyruvica, and acinetobacter lwoffii, as well as strains of neisseria subflava and acinetobacter calcoaceticus. the data in a distance matrix constructed by comparing the sequences suppor ... | 1994 | 7520730 |
| a novel ancestral protein of drosophila alcohol dehydrogenase in streptomyces? | polyclonal antibodies raised against purified drosophila alcohol dehydrogenase (adh) were used in western blot analyses to search for structurally and/or immunologically related proteins in prokaryotes and eukaryotes. no immunological-reactive protein was detected in a flesh fly, a locust, and butterflies. immunological similarity with the 50-kda pqq-glucose dehydrogenase (gludh)-b enzyme of acinetobacter calcoaceticus was found, but the cross-reactivity apparently is dependent on the high hydro ... | 1993 | 7510103 |
| use of a simplified cell blot technique and 16s rrna-directed probes for identification of common environmental isolates. | a simple technique in which rrna-targeted oligodeoxynucleotide probes are used to identify bacteria immobilized on membranes is described. by using colony lifts, bacteria are directly transferred from plates to untreated nitrocellulose membranes. alternatively, cells resuspended from colonies can be applied to membranes by using a vacuum manifold under high-salt conditions. blotted cells are baked and hybridized under stringent conditions by using standard protocols. treatment of blotted cells w ... | 1993 | 7504429 |
| purification and characterization of acinetobacter calcoaceticus 4-hydroxybenzoate 3-hydroxylase after its overexpression in escherichia coli. | 4-hydroxybenzoate 3-hydroxylase [ec 1.14.13.2] from acinetobacter calcoaceticus was purified to homogeneity following the 40-fold overexpression of this gene (poba) in escherichia coli. overexpression was accomplished by placing the fola gene (encoding trimethoprim-resistant dihydrofolate reductase) directly downstream of the poba gene, and demanding growth of recombinants on elevated concentration of trimethoprim. presumably, the surviving variants have undergone a genetic alteration which allo ... | 1995 | 7490269 |
| [the efficacy of sulbactam-ampicillin in a urinary infection due to acinetobacter calcoaceticus var. anitratus]. | 1995 | 7475461 | |
| meningitis caused by acinetobacter calcoaceticus var anitratus. a specific hazard in neurosurgical patients. | acinetobacter calcoaceticus var anitratus caused meningitis in five patients between 1968 and 1978 at two hospitals affiliated with boston university school of medicine. all patients had had head trauma or neurosurgical procedures prior to the development of meningitis. the course of the disease was relatively indolent in that fulminant disease did not occur even when initial therapy was inappropriate and bacteria persisted in csf. all five patients survived. on gram's stain of csf, a calcoaceti ... | 1981 | 7469844 |
| symptomatic acinetobacter calcoaceticus peritonitis. "a complication of peritoneal dialysis". | acinetobacter calcoaceticus peritonitis was seen during the course of peritoneal dialysis in two patients with end-stage kidney disease within a 3-month period. in one patient, the isolate was a. calcoaceticus varient anitratus (formerly herellea vaginicola) and in the other it was a. calcoaceticus lwoffi (formerly mima polymorpha). both were successfully treated with continuous peritoneal antibiotic lavage. | 1980 | 7440841 |
| gene transfer in acinetobacter calcoaceticus: fertility variants of the sex factor pav1. | the naturally occurring transmissible plasmid pav1 mediates chromosome transfer and can exhibit two distinct levels of transmissibility in acinetobacter calcoaceticus strain ebf65/65. the two states of pav1 have been arbitrarily designated pav1a (low frequency variant) and pav1b (high frequency variant). both variants have the same incompatibility and host range properties and each mobilizes two non-transmissible resistance determinants for tetracycline and neomycin. sex factor activity has been ... | 1980 | 7411116 |
| pyrogenic reactions after inadvertent infusion of endotoxin during cardiac catheterizations. | in april 1976 the attack rate of chills and fever with or without falls in blood pressure increased in association with cardiac catheterizations. fevers were associated with coronary angiography and right and left heart catheterizations. blood cultures were negative, and reactions did not correlate with amounts of contrast materials infused or with procedures done by a single operator. significant numbers of acinetobacter calcoaceticus (var. anitratus) and a pseudomonas species were cultured fro ... | 1980 | 7396310 |
| homologies in the nh2-terminal amino acid sequences of gamma-carboxymuconolactone decarboxylases and muconolactone isomerases. | gamma-carboxymuconolactone decarobxylase (ec 4.1.1.44) and muconolactone isomerase (ec 5.3.3.4) mediate chemically analogous reactions in bacteria. the enzymes are inducible, and different metabolites trigger the respective syntheses of the decarboxylases in acinetobacter calcoaceticus and pseudomonas putida. the decarobxylases share similar oligomeric structures in which identical subunits of about 13,300 daltons appear to be self-associated into hexamers. identical residues are found in 18 of ... | 1980 | 7391024 |
| evolutionary divergence of co-selected beta-ketoadipate enol-lactone hydrolases in acinetobacter calcoaceticus. | muconolactone isomerase (ec 5.3.3.4) and beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) mediate consecutive catabolic steps in bacteria. separately inducible beta-ketoadipate enol-lactone hydrolases i and ii are formed in representatives of acinetobacter calcoaceticus. when subjected to deae-cellulose chromatography, acinetobacter enol-lactone hydrolase i displays heterogeneous behavior which, in whole or in part, appears to be due to modifications of sulfhydryl groups in the protein; the ... | 1980 | 7391023 |
| repetitions in the nh2-terminal amino acid sequence of beta-ketoadipate enol-lactone hydrolase from pseudomonas putida. | muconolactone delta-isomerase (ec 5.3.3.4) and beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) mediate consecutive reactions in the beta-ketoadipate pathway of bacteria. an earlier investigation (yeh, w.k., davis, g., fletcher, p., and ornston, l.n. (1978) j. biol. chem. 253, 4920-4923) revealed that the respective nh2-terminal amino acid sequences of pseudomonas putida muconolactone isomerase and acinetobacter calcoaceticus beta-ketoadipate enol-lactone hydrolase ii are evolutionarily hom ... | 1980 | 7391022 |
| naturally occurring plasmids in acinetobacter calcoaceticus: a p class r factor of restricted host range. | a naturally occurring transmissible plasmid, designated pav1, has been isolated in acinetobacter calcoaceticus. it specifies resistance to sulphonamides and is capable of mobilizing two non-transmissible resistance determinants for tetracycline and neomycin, respectively, within strains of a. calcoaceticus. it is incompatible with the p class r factors rp4 and r751 in a. calcoaceticus. on this basis we conclude that pav1 is a member of the p incompatibility group. however, unlike most other p gr ... | 1980 | 7365457 |
| regulation of phosphoenolpyruvate carboxylase from acinetobacter calcoaceticus by nucleotides. | 1980 | 7353640 | |
| evidence of plasmid-mediated production of aminoglycoside-modifying enzymes not previously described in acinetobacter. | two blood culture isolates of acinetobacter calcoaceticus subsp. anitratus (herellea vaginicola) were recently observed to be unusually resistant to aminoglycosides. each strain was found to contain aminoglycoside-modifying enzymes which have not been described previously in this species, including 2"-adenylyltransferase, 3"-adenylyltransferase, 3'-phosphotransferase-iii, and 3-acetyltransferase. treatment of one strain (h-s) with novobiocin led to a loss of resistance to multiple antimicrobial ... | 1980 | 7352749 |
| isolation of acinetobacter calcoaceticus from an aborted equine foetus. | 1981 | 7342938 | |
| acinetobacter calcoaceticus-an opportunistic pathogen. | 1981 | 7338827 | |
| a cluster of acinetobacter pneumonia in foundry workers. | in a 3-month period, three men who had worked for 5 to 19 years as welders or grinders of steel castings in a foundry acquired pneumonia caused by acinetobacter calcoaceticus variety anitratus serotype 7j. two of the men died, and postmortem examination showed mixed-dust pneumoconiosis with iron particles in the lungs. a calcoaceticus variety anitratus serotype 7j was isolated from the air in the foundry but the source was not found. the prevalence of antibody titers of 64 or greater to the 7j s ... | 1981 | 7305146 |
| role of adherence in growth of acinetobacter calcoaceticus rag-1 on hexadecane. | the high affinity of acinetobacter calcoaceticus rag-1 for liquid hydrocarbons permitted the isolation of a spontaneous nonadherent mutant, mr-481. strain mr-481 exhibited no significant affinity for three test hydrocarbons, yet resembled the wild type in many properties, including production of the extracellular emulsifying agent emulsan. to study the role of adherence in growth on hydrocarbons, rag-1 and mr-481 were compared for growth on hexadecane under conditions of limited agitation and at ... | 1981 | 7287631 |
| microbial flora of in-use, display eye shadow testers and bacterial challenges of unused eye shadows. | we surveyed 15 different brands of eye shadow on display for customer use in different retail stores for microbial contamination. this was the first reported microbial surveillance of in-use eye shadow display testers in retail establishments. cultures were obtained at each retail store. sterile dacron swabs were rolled and rubbed over the entire used surface of each shadow, and each inoculum was streaked onto the surfaces of blood agar plates. of the 1,345 individual samples obtained, 67% were ... | 1981 | 7283427 |
| naturally occurring plasmids in acinetobacter calcoaceticus: pav2, a plasmid which influences the fertility of the sex factor pav1. | acinetobacter calcoaceticus strain ebf65/65 harbours a cryptic plasmid, pav2, which has been shown by electrophoretic separation on agarose gels to have a molecular mass of approximately 13.5 megadaltons (md). transfer of the previously described sex factor pav1 (hinchliffe & vivian, 1980 a,b) from the hospital strainjc17 into strains possessing pav2 occurs only at a low frequency, whereas transfer to similar strains lacking pav2 occurs at a much higher frequency. in ebf65/65, pav1 may be presen ... | 1980 | 7264602 |
| adherence of acinetobacter calcoaceticus rag-1 to human epithelial cells and to hexadecane. | the ability of acinetobacter calcoaceticus rag-1 to adhere to human epithelial cells was investigated and compared with its ability to adhere to a test hydrocarbon (hexadecane). rag-1, a microorganism originally isolated for growth on hydrocarbon, adhered to epithelial cells when grown under conditions which promote its adherence to hexadecane; similarly, rag-1 cells adhered poorly to epithelial cells when grown under conditions which cause the cells to possess low affinity towards hexadecane. a ... | 1981 | 7263064 |
| a comparison of the in vitro activity of cefoxitin and cephalothin against 7,312 clinical isolates. | the antibacterial activity of cefoxitin and cephalothin was tested against 7,312 clinical isolates of aerobic pathogens. cefoxitin was found to have a broad spectrum of activity. among the gram-positive cocci, 99.1% of staphylococcus aureus, 94.4% of staphylococcus epidermidis and 98.1% of streptococci group b had a minimal inhibitory concentration of 16 microgram/ml or less, while only 4.6% of enterococci were sensitive to this concentration of cefoxitin. among the gram-negative rods 94.4% of e ... | 1981 | 7249786 |
| [epidemiology of acinetobacter calcoaceticus]. | 1980 | 7243591 | |
| aerobic bacterial oral flora of garter snakes: development of normal flora and pathogenic potential for snakes and humans. | garter snakes that are used for scientific laboratory studies or kept as exotic pets often become ill and die early in captivity. they may also act as reservoirs of potential human pathogens or transmit infection to man. a total of 126 strains of aerobic and facultative bacteria, most potential human and snake pathogens, were isolated from 82 garter snake oropharyngeal cultures. coagulase-negative staphylococcus species were the most common species isolated. acinetobacter calcoaceticus var. anit ... | 1981 | 7240404 |
| influence of growth phase and carbon source on the content of rubredoxin in acinetobacter calcoaceticus. | the rubredoxin content of acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, l-malate) and on the growth phase was studied by means of a radioimmunoassay. the method used was specific for rubredoxin and acinetobacter calcoaceticus. the formation of rubredoxin increased with time up to the end of the logarithmic phase when n-alkanes were the sole carbon source. after growth of acinetobacter calcoaceticus on non-hydrocarbon substrates, rubredoxin was not ... | 1980 | 7212930 |
| infective endocarditis due to acinetobacter calcoaceticus. | 1980 | 7205689 | |
| pneumonia caused by acinetobacter calcoaceticus var. anitratus. | 1982 | 7186001 | |
| sensitivity of 341 non-fermentative gram-negative bacteria to seven beta-lactam antibiotics. | susceptibility of 341 isolates of non-fermentative gram-negative bacteria to carbenicillin, piperacillin, cefoperazone, moxalactam, cefotaxime, ceftizoxime, and n-formimidoyl thienamycin was determined by the agar dilution and disc diffusion methods. piperacillin was the most active agent against pseudomonas aeruginosa, thienamycin the most active against pseudomonas fluorescens and pseudomonas putida, and moxalactam the most active against pseudomonas maltophilia. piperacillin and thienamycin w ... | 1982 | 7173181 |