Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| uptake and processing of dna by acinetobacter calcoaceticus--a review. | in natural transformation, dna in the form of macromolecular fragments can be translocated across the cell envelope of prokaryotic microorganisms. during the past two decades, several, largely mutually contradictory, hypotheses have been forwarded to explain the molecular mechanism and bioenergetics of this translocation process. other biomacromolecules are translocated across the bacterial cell envelope as well, such as polysaccharides and proteins, the latter for instance in the process of the ... | 1997 | 9224889 |
| antibacterial activity of trovafloxacin against nosocomial gram-positive and gram-negative isolates. | a total of 1132 clinical isolates from 952 patients with nosocomial infections were tested against the fluoroquinolone trovafloxacin by the agar dilution method. they comprised 285 staphylococci, 111 streptococci, 94 enterococci and 470 isolates of enterobacteriaceae, 92 pseudomonas aeruginosa, 27 stenotrophomonas maltophilia, 28 haemophilus influenzae and 25 acinetobacter calcoaceticus. the in-vitro activity of trovafloxacin was compared with that of ciprofloxacin, norfloxacin, beta-lactam and ... | 1997 | 9222067 |
| [exposure to organic dust and microorganisms as a factor affecting respiratory function of workers of purebred horse farms]. | in two farms of purebred horses, microbiological studies of the air and medical examination of the workers were performed. the concentration of microorganisms in the air were within the range 29.2-336.0 cfu x 10(3)/m3. the respirable fraction in most cases was between 30-60% of the total count. the levels of dust and endotoxin were low except for one sampling point where the concentration of endotoxin was as high as 3.44 g/m3. as many as 16 workers out of the total of 31 examined reported occurr ... | 1996 | 9190233 |
| the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis. | from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ... | 1996 | 9147724 |
| antibacterial activity of bms-180680, a new catechol-containing monobactam. | the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ... | 1997 | 9145861 |
| the toxicity of substituted phenolic compounds to a detoxifying and an acetic acid bacterium. | in the detoxifying bacterium acinetobacter calcoaceticus 69-v and in the acetic acid bacterium acetobacter methanolicus mb 58, glucose and xylose are oxidized, respectively, via pqq-dependent membrane-bound dehydrogenases, which are linked to the respiratory chain in a manner enabling energy conservation via electron transport phosphorylation (etp) in the cytoplasmic membrane. neither the glucose and gluconic acid nor the xylose and xylonic acid are metabolized. therefore, measurements of sugar ... | 1997 | 9143455 |
| natural transformation and availability of transforming dna to acinetobacter calcoaceticus in soil microcosms. | a small microcosm, based on optimized in vitro transformation conditions, was used to study the ecological factors affecting the transformation of acinetobacter calcoaceticus bd413 in soil. the transforming dna used was a. calcoaceticus homologous chromosomal dna with an inserted gene cassette containing a kanamycin resistance gene, nptii. the effects of soil type (silt loam or loamy sand), bacterial cell density, time of residence of a. calcoaceticus or of dna in soil before transformation, tra ... | 1997 | 9143126 |
| isolation of mutants of acinetobacter calcoaceticus deficient in wax ester synthesis and complementation of one mutation with a gene encoding a fatty acyl coenzyme a reductase. | acinetobacter calcoaceticus bd413 accumulates wax esters and triacylglycerol under conditions of mineral nutrient limitation. nitrosoguanidine-induced mutants of strain bd413 were isolated that failed to accumulate wax esters under nitrogen-limited growth conditions. one of the mutants, wow15 (without wax), accumulated wax when grown in the presence of cis-11-hexadecenal and hexadecanol but not hexadecane or hexadecanoic acid. this suggested that the mutation may have inactivated a gene encoding ... | 1997 | 9139916 |
| sequence and functional analysis of an escherichia coli dna fragment able to complement pqqe and pqqf mutants from methylobacterium organophilum. | a 7361 kb fragment of e coli chromosomal dna able to complement pqqe and pqqf mutants of methylobacterium organophilum has been sequenced. five open reading frames (orf) have been identified. four orfs (102, 103, 106 and 107), belong to a single transcription unit. they are separated by a transcription termination site from a fifth orf (orf109). polypeptides of 28, 85 and 82 kda encoded by orfs 102, 103 and 106 respectively were visualised in maxi-cell experiments. both orf106 and orf107 are req ... | 1996 | 9116051 |
| bioengineering of emulsifier structure: emulsan analogs. | strategies were investigated to modulate the side chain structure of emulsans formed by acinetobacter calcoaceticus rag-1. analysis of emulsan fatty acid side chain groups by gas chromatography--mass spectrometry (gc-ms) revealed that by provoking the exogenous n-alkanoic fatty acids 15:0, 16:0, and 17:0, emulsan analogs were formed with 53, 46, and 44 mol%, respectively, of fatty acid substituents with chain lengths equal to that of the carbon source. in contrast, the increase in emulsan fatty ... | 1997 | 9115094 |
| incorporation of 2-hydroxyl fatty acids by acinetobacter calcoaceticus rag-1 to tailor emulsan structure. | acinetobacter calcoaceticus rag-1 was cultured on different chain length saturated 2-hydroxyl fatty acid (2-hofa) carbon sources as follows: c12:0 (2-oh), c14:0 (2-oh), c16:0 (2-oh) and c18:0 (2-oh). these 2-hofas were used as either sole carbon sources or cosubstrates with c14:0 (total 1% w/v) to form new emulsans (ems) having controlled side chain fa structure and, therefore, unique emulsifier characteristics. em yields and cell dry weights ranged from 0.6 to 1.8 g/l and 0.9 to 3.9 g/l, respec ... | 1997 | 9110181 |
| activities of beta-lactams against acinetobacter genospecies as determined by agar dilution and e-test mic methods. | the agar dilution mic method was used to test activities of ticarcillin, ticarcillin-clavulanate, amoxicillin, amoxicillin-clavulanate, ampicillin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, inhibitors alone, ceftazidime, and imipenem against 237 acinetobacter genospecies. a total of 93.2% of strains were beta-lactamase positive by the chromogenic cephalosporin method. overall, ampicillin-sulbactam was the most active combination against all strains (mic at which 50% of the iso ... | 1997 | 9087486 |
| nucleotide sequence of a putative periplasmic mn superoxide dismutase from acinetobacter calcoaceticus adp1. | we determined 5.8 kilobases of nucleotide sequence upstream of the rubredoxin encoding ruba gene of acinetobacter calcoaceticus (ac) adp1. sequence analysis revealed four open reading frames named cysd', cobq, soda and lyss, coding for proteins with high similarity to known sulfate adenylate transferases (partial), cobyric acid synthases, superoxide dismutases (sod) and lysyl trna synthetases, respectively. out of a large number of bacterial sod sequences soda of ac adp1 is the first member of t ... | 1997 | 9074511 |
| oral treatment of staphylococcus spp. infected orthopaedic implants with fusidic acid or ofloxacin in combination with rifampicin. | oral therapy of staphylococcal infection of orthopaedic implants with 900 mg/day rifampicin combined with either 1.5 g/day fusidic acid for 5 days followed by 1 g/day thereafter, or 600 mg/day ofloxacin was compared. patients with an infected hip were treated for 6 months, with removal of any unstable prosthesis after 5 months' treatment and those with an infected knee prosthesis were treated for 9 months, with removal of the prosthesis after 6 months of treatment. patients with infections of ot ... | 1997 | 9069545 |
| an apparent outbreak of infection with acinetobacter calcoaceticus reconsidered after investigation by pyrolysis mass spectrometry. | in a previously reported apparent outbreak of infection due to acinetobacter calcoaceticus on a regional burns unit in which both clinical and environmental isolates were available for study, investigations of the organisms by electrophoretic typing of surface proteins, plasmid profiling and antibiograms were inconclusive and unable to identify a single epidemic strain of the organism. the same collection of isolates has been analysed for inter-strain comparison by pyrolysis mass spectrometry (p ... | 1997 | 9060155 |
| sigma54, a vital protein for myxococcus xanthus. | the rpon gene encoding the transcription factor sigma54 in myxococcus xanthus has been cloned using a heterologous rpon probe. the sequence of the cross-hybridizing dna confirmed the existence of an orf 1518 bp long that encodes a well conserved member of the sigma54 family of sigma factors. low- as well as high-stringency hybridizations detected only a single rpon gene in the m. xanthus chromosome. in other bacteria, sigma54 is an alternative sigma, and null mutants are viable. however, all att ... | 1997 | 9050890 |
| sequence analysis of pqq genes required for biosynthesis of pyrroloquinoline quinone in methylobacterium extorquens am1 and the purification of a biosynthetic intermediate. | methylobacterium extorquens am1 produces pyrroloquinoline quinone (pqq), the prosthetic group of methanol dehydrogenase. two genes clusters have been shown to be required for pqq biosynthesis in this micro-organism and complementation analysis has identified seven pqq genes, pqqdgcba and pqqef. the dna sequence of pqqdgc' was reported previously. this paper reports the sequence of the genomic region corresponding to pqqc'ba. for consistency, the nomenclature of pqq genes in klebsiella pneumoniae ... | 1997 | 9043136 |
| [reactive arthritis: terminology, etiology and pathogenesis]. | different serovars of y. enterocolitica, y. pseudotuberculosis, acinetobacter calcoaceticus, and pseudomonas pauimobilis were isolated from the synovial fluid of 23 out of 34 patients with yersinia-triggered arthritis by a new bacteriological method based on the selection of the optimal conditions for microorganism culturing; in some cases the strains were isolated repeatedly. the authors discuss the necessity of correcting the previous notions on the aseptic nature of reactive arthritis. | 1996 | 9036205 |
| [bactericide activity of sulbactam before bacteria belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex]. | to evaluate the bactericidal activity of colistin, imipenem and sulbactam against 24 acinetobacter calcoaceticus-acinetobacter baumannii complex isolations. | 1996 | 9035707 |
| structural and serological characterisation of the o-specific polysaccharide from lipopolysaccharide of acinetobacter calcoaceticus strain 7 (dna group 1). | s-form lipopolysaccharide was isolated by phenol/water extraction from a strain of acinetobacter calcoaceticus (dna group 1 ). the structure of the o-antigenic polysaccharide was determined by compositional analysis and nmr spectroscopy of the de-o-acylated lipopolysaccharide. the isolated polysaccharide obtained after hydrolysis of lipopolysaccharide in 0.01 m trifluoroacetic acid has the following structure: [structure in text] in which pyr is pyruvate. the o-acetyl substitution of d-gal was n ... | 1997 | 9030736 |
| expression, inducer spectrum, domain structure, and function of mopr, the regulator of phenol degradation in acinetobacter calcoaceticus ncib8250. | degradation of phenol by acinetobacter calcoaceticus ncib8250 involves (sigma54-dependent expression of a multicomponent phenol hydroxylase and catechol 1,2-dioxygenase encoded by the mop operon. complementation of a new mutant deficient in phenol utilization yielded the regulatory locus mopr. it is located in divergent orientation next to the mop operon. mopr is constitutively expressed at a low level from a sigma70-type promoter and belongs to the ntrc family of regulators. the amino acid sequ ... | 1997 | 9023219 |
| microbiology of otitis externa. | microbiologic and clinical data from 26 patients with otitis externa were prospectively evaluated. specimens were processed for aerobic and anaerobic bacteria. bacterial growth was noted in 23 specimens. a total of 33 aerobic and 2 anaerobic bacteria were recovered. aerobic bacteria only were isolated in 21 (91%) patients, anaerobic bacteria only in 1 (4%), and mixed aerobic and anaerobic bacteria in 1 (4%). the most common isolates were pseudomonas aeruginosa (14 instances), staphylococcus aure ... | 1997 | 9018252 |
| [microbial associations in trichomonas urethritis]. | the results on isolation of associated microflora from 190 patients suffering from trichomonas urethritis are given. microorganisms belonged to the species: mycoplasma hominis. ureaplasma urealyticum. streptococcus pyogenes. escherichia coli. klebsiella pneumoniae. proteus mirabilis. moraxella lacunata. acinetobacter calcoaceticus and candida. staphylococcus cultures which were isolated from 100% patients belonged to 8 species: staphylococcus aureus, s. intermedius, s. haemolyticus, s. epidermid ... | 1996 | 8983524 |
| identification and characterization of a pantoea citrea gene encoding glucose dehydrogenase that is essential for causing pink disease of pineapple. | pantoea citrea, a member of the family enterobacteriaceae, causes pink disease of pineapple, whose symptom is characterized by the formation of pink to brown discolorations of the infected portions of the pineapple fruit cylinder upon canning. molecular genetic approaches were applied to elucidate the mechanism responsible for this fruit discoloration. a p. citrea mutant strain, cmc6, defective in its ability to cause pink disease and fruit discoloration, was generated by nitrosoguanidine mutage ... | 1997 | 8979341 |
| [microbiology of femoral head grafts in bone banks]. | the aim of the study was to describe the bacteriology of bone allografts, which were harvested for the bone bank at hvidovr hospital, and to evaluate any infections in the recipients, which may have been caused by microbiological contamination of the transplanted bone allografts. it was carried out as a retrospective examination of bone bank records and patient records allowing at least 12 months for possible manifestation of bacterial infection by the bone allograft. during donation of bone, bo ... | 1996 | 8966808 |
| novel nuclear magnetic resonance spectroscopy methods demonstrate preferential carbon source utilization by acinetobacter calcoaceticus. | novel nuclear magnetic resonance spectroscopy techniques, designated metabolic observation, were used to study aromatic compound degradation by the soil bacterium acinetobacter calcoaceticus. bacteria which had been rendered spectroscopically invisible by growth with deuterated (2h) medium were used to inoculate cultures in which natural-abundance 1h hydrogen isotopes were provided solely by aromatic carbon sources in an otherwise 2h medium. samples taken during the incubation of these cultures ... | 1996 | 8955304 |
| production, characterization, and reconstitution of recombinant quinoprotein glucose dehydrogenase (soluble type; ec 1.1.99.17) apoenzyme of acinetobacter calcoaceticus. | soluble, periplasmic quinoprotein glucose dehydrogenase of acinetobacter calcoaceticus (sgdh; ec 1.1.99.17) was produced in good yield in the apoenzyme form (without the cofactor pyrroloquinoline quinone, pqq) by an escherichia coli recombinant strain provided with a plasmid containing the gene under control of a lac promoter. structural analysis of the purified apoenzyme revealed that the e. coli strain used produces the correct mature protein. titration of the apoenzyme with pqq in the presenc ... | 1996 | 8951033 |
| influence of relative humidity and suspending menstrua on survival of acinetobacter spp. on dry surfaces. | acinetobacter spp. are being reported with increasing frequency as a cause of nosocomial infection and have been isolated from the skin of healthy individuals, patients, hospital staff, dry nonbiotic objects, and different pieces of medical equipment. factors affecting the survival of acinetobacter spp. under conditions closely similar to those found in the hospital environment were investigated in the present study to help us understand the epidemiology of nosocomial acinetobacter infection. ba ... | 1996 | 8940416 |
| phosphate release and uptake by pure cultures of acinetobacter sp.: effect of the volatile fatty acids concentration | phosphorus release and uptake by pure cultures of acinetobacter strains were investigated under anaerobic and aerobic conditions respectively. tests were performed to study the relationship between phosphorus release-storage reaction and behavior of extracellular organic substrates: acetic, propionic, and butyric acids have been used at four concentrations (50, 100, 500, and 1000 mg · l-1) in the anaerobic step of biological phosphorus removal. the results obtained depend on the strain an ... | 1997 | 8939801 |
| the expression of the acinetobacter calcoaceticus reca gene increases in response to dna damage independently of reca and of development of competence for natural transformation. | using the lacz operon fusion technique, the transcriptional control of the acinetobacter calcoaceticus reca gene was studied. a low (approximately twofold) inductive capacity was observed for compounds that damage dna and/or inhibit dna replication, e.g. methyl methanesulfonate, mitomycin c, uv light and nalidixic acid. induction of the reca gene by dna damage was independent of functional reca. the presence of the reca promoter region on a multicopy plasmid had the same effect on reca transcrip ... | 1996 | 8936328 |
| system to study horizontal gene exchange among microorganisms without cultivation of recipients. | ribosomal rna genes are characterized by highly conserved sequences and are present in multiple copies in most prokaryotic chromosomes. in principle, therefore, they might serve as sites for homologous recombination between unrelated microorganisms. plasmids containing 23s ribosomal gene sequences, from different bacteria, which had been interrupted by insertion of a kanamycin-resistance gene, were used to transform acinetobacter sp. dsm587 (former name: acinetobacter calcoaceticus bd413-ivl10). ... | 1996 | 8930906 |
| [simultaneous isolation of mrsa and pseudomonas aeruginosa using a novel selective and differential pmac agar]. | pmac agar, a novel, selective and differential medium has been developed and was subjected for evaluation of its selective and differential capability of methicillin resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa from other bacteria such as bacillus, micrococcus, gram-negative bacteria and drug resistant ones. growth of mrsa and p. aeruginosa on pmac agar was facilitated and their colonies were easily differentiated. colonies of mrsa after 24 approximately 48 h incubation at 3 ... | 1996 | 8921677 |
| antibiotic resistance pattern and r-plasmids of acinetobacter calcoaceticus subsp. anitratus. | during a 6 month period from march 1990 to august 1990, a total of 159 strains of acinetobacter calcoaceticus subsp. anitratus were isolated from various samples and studied for antibiotic resistance pattern to 12 drugs by kirby-bauer method. ceftazidime and netilmycin were the most sensitive drugs followed by cefotaxime, norfloxacin and augmentin. all the strains were resistant to chloramphenicol and tetracycline. commonest pattern of resistance was acgkstsu. forty eight isolates were tested fo ... | 1995 | 8919107 |
| the beta-ketoadipate pathway and the biology of self-identity. | the beta-ketoadipate pathway is a chromosomally encoded convergent pathway for aromatic compound degradation that is widely distributed in soil bacteria and fungi. one branch converts protocatechuate, derived from phenolic compounds including p-cresol, 4-hydroxybenzoate and numerous lignin monomers, to beta-ketoadipate. the other branch converts catechol, generated from various aromatic hydrocarbons, amino aromatics, and lignin monomers, also to beta-ketoadipate. two additional steps accomplish ... | 1996 | 8905091 |
| multiparametric analysis of waterline contamination in dental units. | microbial contamination of dental unit waterlines is thought to be the result of biofilm formation within the small-bore tubing used for these conduits. systematic sampling of 121 dental units located at the dental school of université de montréal showed that none of the waterlines was spared from bacterial contamination. multilevel statistical analyses showed significant differences between samples taken at the beginning of the day and samples taken after a 2-min purge. differences were also fo ... | 1996 | 8899982 |
| degradation of crude oil by acinetobacter calcoaceticus and alcaligenes odorans. | two types of indian crude oil (bombay high and gujarat) were tested for their biodegradability by acinetobacter calcoaceticus and alcaligenes odorans. acinetobacter calcoaceticus s30 and alc. odorans p20 degraded bombay high crude oil by 50% and 45%, while only 29% and 37% of gujarat crude oil (heavy crude oil) was degraded by these isolates, respectively. acinetobacter calcoaceticus and alc. odorans in combination degraded 58% and 40% of bombay high and gujarat crude oils, respectively, which w ... | 1996 | 8896350 |
| increased production of recombinant pyrroloquinoline quinone (pqq) glucose dehydrogenase by metabolically engineered escherichia coli strain capable of pqq biosynthesis. | we have previously shown that the production of recombinant escherichia coli pqqgdh was greatly improved by using a medium supplemented with the cofactor pqq, which is not synthesized in e. coli. we show here that the increase in the accumulated pqqgdh is due to the increased stability of the holo-enzyme over apo-enzyme, using recombinant acinetobacter calcoaceticus pqqgdh. in order to achieve cost-effective pqqgdh production, we incorporated the genes for pqq biosynthetic pathway from klebsiell ... | 1996 | 8879174 |
| inhibition studies of s-adenosylhomocysteine hydrolase purified from acinetobacter calcoaceticus ula-501. | adenosine analogs previously reported as reversible inhibitors of mammalian s-adenosylhomocysteine hydrolase (sahase) have been found to exert similar effects on acinetobacter calcoaceticus ula-501 enzyme. in addition, two metal coordination compounds, cis-platinum diammine dichloride (cis-ddp) and its trans isomer (trans-ddp), the former well known for its employment in anticancer chemotherapy, were assayed on both bacterial and mammalian sahases. in our conditions, trans-ddp appeared to be the ... | 1996 | 8874802 |
| purification of a lipase from acinetobacter calcoaceticus aac323-1 by hydrophobic-interaction methods. | the performance of hydrophobic-interaction chromatography (hic) for the purification of acinetobacter calcoaceticus aac323-1 lipase was compared with that of various aqueous two-phase systems. while a 42% lipase yield with a purification factor of 140 could be recovered by hic, higher yields were achieved by using aqueous two-phase systems, either those formed by poly(ethylene glycol) and dextran or those based upon the use of a detergent. triton x-114-based aqueous two-phase partition showed th ... | 1996 | 8867899 |
| in vitro and in vivo activities of lb10522, a new catecholic cephalosporin. | in vitro activity of lb10522 was compared with those of cefpirome, ceftazidime, ceftriaxone, and cefoperazone against clinical isolates. against gram-positive bacteria, lb10522 was most active among the compounds tested. it was fourfold more active than cefpirome against methicillin-susceptible staphylococcus aureus and enterococcus faecalis. lb10522 was highly effective against most members of the family enterobacteriaceae tested. ninety percent of isolates of escherichia coli, klebsiella oxyto ... | 1996 | 8843288 |
| emergence of resistant isolates of acinetobacter calcoaceticus- a. baumannii complex in a spanish hospital over a five-year period. | acinetobacter calcoaceticus-a. baumannii complex species have emerged as a relevant cause of nosocomial infection and colonization over the past 20 years, mainly in intensive care units. the aim of this study was to investigate the in vitro activity of 14 antimicrobial agents against 177 clinical isolates from patients admitted to a spanish teaching hospital over a five-year period. susceptibility rates or 99%, 99%, and 74% were obtained for imipenem, meropenem, ampicillin plus sulbactam, and am ... | 1996 | 8839649 |
| physiological factors affecting production of extracellular lipase (lipa) in acinetobacter calcoaceticus bd413: fatty acid repression of lipa expression and degradation of lipa. | the extracellular lipase (lipa) produced by acinetobacter calcoaceticus bd413 is required for growth of the organism on triolein, since mutant strains that lack an active lipase fail to grow with triolein as the sole carbon source. surprisingly, extracellular lipase activity and expression of the structural lipase gene (lipa), the latter measured through lacz as a transcriptional reporter, are extremely low in triolein cultures of lipa+ strains. the explanation for this interesting paradox lies ... | 1996 | 8830702 |
| ptim3, a plasmid delivery vector for a transposon-based inducible marker gene system in gram-negative bacteria. | ptim3 is a suicide plasmid vector for the delivery of a transposition defective derivative of tn5, expressing inducible mercury resistance (hgr) and catechol 2,3-dioxygenase (c23o) activity, to a range of gram-negative microorganisms. ptim3 was constructed by a four-stage process from pnmm1, a derivative of psup5011 containing a modified tn5 where antibiotic-resistance determinants have been replaced by the mer operon of tn501 and tdnc (encoding a c23o). in ptim3, tdnc is fused to merd, to bring ... | 1995 | 8825369 |
| changing trends in the antimicrobial susceptibility of clinical isolates of acinetobacter baumannii. | acinetobacter baumannii has recently emerged as an important nosocomial pathogen because of its resistance to the majority of commonly used antimicrobial agents. therefore, the pattern of isolation of acinetobacter baumannii in our laboratory and the in vitro susceptibilities to various antimicrobial agents were studied. one hundred and two clinical isolates of previously identified acinetobacter calcoaceticus var. anitratus from different patients were collected from the diagnostic bacteriology ... | 1996 | 8799001 |
| [in vitro and in vivo antibacterial activities of sulopenem, a new penem antibiotic]. | the in vitro and in vivo antibacterial activities of sulopenem, a new penem, were evaluated in comparison with imipenem (ipm), meropenem (mepm), ceftazidime (caz) and flomoxef (fmox). sulopenem had broad and potent antibacterial spectra against gram-positive and gram-negative bacteria, including enterococcus faecalis, proteus vulgaris, morganella morganii, enterobacter spp. and citrobacter freundii. sulopenem showed concentration-dependent bactericidal activities against staphylococcus aureus, e ... | 1996 | 8786626 |
| [in vitro antibacterial activity of a new parenteral penem, sulopenem]. | eighty percent minimum inhibitory concentrations (mic80) of sulopenem against clinically isolated 12 to 80 strains of each of different bacteria were as follows: methicillin-susceptible staphylococcus aureus (mssa): 0.20 micrograms/ml, methicillin-resistant s. aureus (mrsa): 50 micrograms/ml, coagulase-negative staphylococci: 3.13 micrograms/ml, streptococcus pyogenes: < or = 0.013 microgram/ml, streptococcus pneumoniae: < or = 0.013 microgram/ml, beta-streptococci: 0.05 microgram/ml, enterococc ... | 1996 | 8786624 |
| evaluation of the ability of a commercial system to identify acinetobacter genomic species. | a collection of 130 acinetobacter strains identified by dna hybridization to 18 different genomic species was used to assess the ability of the api 20ne system (biomérieux, france) to identify acinetobacter genomic species and to determine its accuracy. fifty-eight (87%) of the 67 strains of genomic species defined in the database (version 5.1) were identified to the appropriate genomic species. the acinetobacter baumannii strains and the acinetobacter haemolyticus strains were all identified co ... | 1996 | 8781881 |
| a reappraisal of the diversity and class distribution of aspartate transcarbamoylases in gram-negative bacteria. | recently, the subunit composition of class a aspartate transcarbamoylases (atcases) in fluorescent pseudomonads has been clarified. we present evidence that distribution of this type of atcase may be more widespread than at first suspected. bacterial atcases exist in three forms: class a (molecular mass approximately 450-500 kda); class b, typified by escherichia coli atcase (approximately 300 kda); and class c, typified by bacillus subtilis atcase (approximately 100 kda). using gradient gel ele ... | 1996 | 8757751 |
| is1236, a newly discovered member of the is3 family, exhibits varied patterns of insertion into the acinetobacter calcoaceticus chromosome. | analysis of spontaneous mutations in acinetobacter calcoaceticus revealed a 1237 bp insertion sequence named is1236 and possessing a nucleotide sequence resembling those of members of the ls3 family. the chromosome of a. calcoaceticus strain adp1 contains seven copies of is1236 which appears to insert preferentially into pobr, the transcriptional activator of the structural gene for p-hydroxybenzoate hydroxylase. is1236 creates tandem 3 bp dna duplications flanking the sites of its insertion in ... | 1996 | 8757745 |
| [identification and typing of hospital strains of acinetobacter calcoaceticus-acinetobacter baumanni complex]. | a collection of 95 strains of the acinetobacter calcoaceticus-acinetobacter baumannii complex, isolated between 1991 and 1993 in the prague burn center (bc), was studied. ninety-one strains were isolated from 43 patients: 50 of them from burnt sites, 22 from endotracheal tube, 13 from urine, 3 from blood and 3 from venous catheter, and 4 strains were isolated from the hospital environment and the nursing staff. the strains were classified by restriction endonuclease fingerprinting of total dna, ... | 1996 | 8756404 |
| microbial colonization of large wounds. | this study determines the nature of microbial wound colonization in 28 patients with large burns admitted to the burn centre, haukeland university hospital, bergen. altogether, 748 swabs were taken in 141 sampling procedures. a total of 414 microbial isolates were detected and their resistance patterns to a variety of systemic antimicrobial agents determined. the most frequent isolates were coagulase-negative staphylococci (21.5 per cent) and staphylococcus aureus (14 per cent), followed by ente ... | 1995 | 8747728 |
| validation of use of whole-cell repetitive extragenic palindromic sequence-based pcr (rep-pcr) for typing strains belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex and application of the method to the investigation of a hospital outbreak. | acinetobacter spp. are being reported with increasing frequency as causes of nosocomial infection. in order to identify reservoirs of infection as quickly as possible, a rapid typing method that can differentiate epidemic strains from environmental and nonepidemic strains is needed. in 1993, a cluster of acinetobacter baumannii isolates from five patients in the adult intensive therapy unit of our tertiary-care teaching hospital led us to develop and optimize a rapid repetitive extragenic palind ... | 1996 | 8727902 |
| in vitro activity of the tricyclic beta-lactam gv104326. | gv104326 is a novel tricyclic beta-lactam (a trinem or, formerly, tribactam). the in vitro activity of gv104326 was compared with those of cefuroxime, cefixime, amoxicillin, amoxicillin-clavulanic acid, cefpirome, and ciprofloxacin. gv104326 had in vitro activity generally similar to that of cefixime against members of the family enterobacteriaceae (mic at which 90% of the isolates are inhibited [mic90], < or = 2 micrograms/ml), with cefuroxime and amoxicillin-clavulanic acid being 8- to 32-fold ... | 1996 | 8723475 |
| natural transformation in river epilithon. | natural transformation was demonstrated in unenclosed experiments incubated in river epilithon. strains of acinetobacter calcoaceticus were transformed to prototrophy by either free dna (lysates) or live donor cells. the sources of transforming dna and recipient culture were immobilized on filters, secured to stones, and incubated midstream in the river. the transfer frequency generally increased with temperature. no transfer was detected in the river taff below 10 degrees c. the age of the reci ... | 1996 | 8702292 |
| cloning of catbcijfd genes for catechol degradation into chromosomal poba and genetic stability of the recombinant acinetobacter calcoaceticus. | a possible obstacle in the development of hybrid strains of acinetobacter calcoaceticus by the introduction of a metabolic pathway into the chromosome is genetic instability of the resulting recombinant strains. therefore, the possibility that the poba gene can be used as a chromosomal cloning site where the transposed genes can be maintained and expressed, was explored in this study. for this purpose, two model hybrid strains of a. calcoaceticus were created, in which a dna fragment carrying ca ... | 1996 | 8695911 |
| cloning and expression of dipeptidase from acinetobacter calcoaceticus atcc 23055. | the gene encoding dipeptidase was cloned from acinetobacter calcoaceticus atc 23055. determination of the nucleotide sequence revealed that the gene had an open reading flame of 1,050 bp coding a protein of 350 amino acids. the deduced amino acid sequence showed 48.8% similarity to human renal dipeptidases and conserved two amino acid residues identified in human and pig renal dipeptidases as essential ones for the catalytic activity. purified recombinant enzyme expressed in escherichia coli did ... | 1995 | 8690717 |
| antibiotic resistance of acinetobacter calcoaceticus strains isolated from patients treated in intensive care units. | the distribution according to specimens and susceptibility to antimicrobial agents of 481 acinetobacter calcoaceticus strains isolated from patients treated in intensive care units were studied. they occurred most frequently in tracheal specimens and pus. using disk diffusion test the strains proved to be multiple resistant to ampicillin (86.3%), azlocillin (86.7%), mezlocillin (84.0%), cefamandole (99.7%), cefoxitin (94.1%), cefuroxime (90.6%), cefoperazone (84.9%), cefotaxime (82.0%), ceftriax ... | 1995 | 8689090 |
| a comparative study of different pcr-based dna fingerprinting techniques for typing of the acinetobacter calcoaceticus-a. baumannii complex. | different pcr-based dna fingerprinting techniques were evaluated for typing 26 clinical isolates belonging to the acinetobacter calcoaceticus-a. baumannii complex. seven isolates belonged to a previously defined outbreak while 19 isolates were unrelated epidemiologically. the pcr-based dna fingerprinting techniques used were: (i) repetitive extragenic palindromic (rep) pcr; (ii) enterobacterial repetitive intergenic consensus (eric) pcr; (iii) randomly amplified polymorphic dna with m13 forward ... | 1996 | 8636966 |
| genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in acinetobacter calcoaceticus ncib8250. | we have mutated acinetobacter calcoaceticus ncib-8250 to growth deficiency on phenol as sole carbon source and isolated genes with similarity to phenol hydroxylase and catechol 1,2-dioxygenase by complementation. sequence analysis reveals the presence of six open reading frames (orfs) with similarities to a pseudomonas multicomponent phenol hydroxylase which are followed by an orf with similarity to cata from a. calcoaceticus adp1. transformation of these genes to adp1 confers the ability to gro ... | 1995 | 8596453 |
| infection of the pancreatic duct following pancreas transplantation with bladder drainage. | combined kidney-pancreas transplantation represents a widely accepted therapy for endstage diabetic nephropathy. drainage of the pancreatic juice into the urinary bladder is the preferred surgical technique in most centers. between january 1987 and december 1994 a total of 85 pancreas transplantations with pancreatocystostomy were performed at the innsbruck university hospital. in all cases a polyvinylcatheter was placed into the pancreatic duct and drained transvesically to the exterior. during ... | 1995 | 8596128 |
| study of a hydrocarbon-utilizing and emulsifier-producing acinetobacter calcoaceticus strain isolated from heating oil. | twenty bacterial strains were isolated from a sample of contaminated heating oil and screened for their ability to use petroleum and several common fuels as the sole source of carbon and energy. one of the isolates, named mm5, was able to grow on petroleum derivatives and brought about an emulsification of those compounds. gas chromatography studies showed that strain mm5 was able to degrade hydrocarbons of heating oil. mm5 has been tentatively identified as a strain of acinetobacter calcoacetic ... | 1995 | 8588840 |
| the reca protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of recas and 16s rrnas from the same species. | the evolution of the reca protein was analyzed using molecular phylogenetic techniques. phylogenetic trees of all currently available complete reca proteins were inferred using multiple maximum parsimony and distance matrix methods. comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. the reca trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the alpha, ... | 1995 | 8587109 |
| differentiation of acinetobacter calcoaceticus sensu stricto from related acinetobacter species by electrophoretic polymorphism of malate dehydrogenase, glutamate dehydrogenase and catalase. | acinetobacter baumannii, unnamed acinetobacter species 3 (studied by p.j.m. bouvet and p.a.d. grimont) and unnamed dna group 13 (studied by i. tjernberg and j. ursing) are the most prevalent acinetobacter species in hospitals. using the identification scheme of bouvet and grimont, it is sometimes difficult to differentiate these species from a. calcoaceticus sensu stricto, a species of the natural environment that has seldom been found associated with human infection. genetically identified acin ... | 1995 | 8584799 |
| phenol degradation by acinetobacter calcoaceticus ncib 8250. | acinetobacter calcoaceticus ncib 8250 utilizes phenol as sole source of carbon and energy via an ortho-cleavage pathway. the presence of ethanol in mixed substrate cultivations repressed the utilization of phenol. in fed batch cultivation the phenol tolerance was increased at least 2-fold. maximum degradation rates of 150 mg phenol/(1 h) and 280 mg phenol/(g h), respectively were observed. phenol hydroxylase is induced by its substrate and in parallel the catechol-1,2-dioxygenase is detectable. ... | 1995 | 8568644 |
| acinetobacter endocarditis in children: a case report and review of the literature. | acinetobacter calcoaceticus, a gram-negative bacterium ubiquitous in soil, water and sewage, is a rare cause of endocarditis in children. the first case of acinetobacter endocarditis in an infant is described. this patient had underlying tetralogy of fallot with absent pulmonary valve. a review of the literature in english revealed only four other cases of acinetobacter endocarditis in children; three of whom had underlying congenital heart disease. like the other reported cases, this patient re ... | 1995 | 8557392 |
| tolerance to acid in ph 5.0-grown organisms of potentially pathogenic gram-negative bacteria. | a wide range of potentially pathogenic species of gram-negative bacteria were far more resistant to extreme acidity (ph 2.0-3.5) when cultured at ph 5.0 (habituated to acid) than after ph 7.0 culture. the differences were particularly great for citrobacter spp., enterobacter spp., klebsiella spp. and for vibrio parahaemolyticus; substantial habituation was also observed for proteus mirabilis and aeromonas formicans but the effect was less marked for serratia marcescens and acinetobacter calcoace ... | 1995 | 8554762 |
| tn5-directed cloning of pqq genes from pseudomonas fluorescens cha0: mutational inactivation of the genes results in overproduction of the antibiotic pyoluteorin. | pseudomonas fluorescens cha0 produces several secondary metabolites, e.g., the antibiotics pyoluteorin (plt) and 2,4-diacetylphloroglucinol (phl), which are important for the suppression of root diseases caused by soil-borne fungal pathogens. a tn5 insertion mutant of strain cha0, cha625, does not produce phl, shows enhanced plt production on malt agar, and has lost part of the ability to suppress black root rot in tobacco plants and take-all in wheat. we used a rapid, two-step cloning-out proce ... | 1995 | 8526497 |
| antibacterial properties of am-1155, a new 8-methoxy quinolone. | am-1155 is a new 8-methoxy quinolonecarboxylic acid with a broad spectrum of antibacterial activity. it inhibited more than 90% of clinical isolates of methicillin-susceptible staphylococcus aureus, streptococci, enterococcus faecalis, most of the enterobacteriaceae, acinetobacter calcoaceticus and haemophilus influenzae at the concentration of 0.39 mg/l. am-1155 was 2- to 16-fold more active than ciprofloxacin against gram-positive organisms including methicillin-resistant staphylococci. the an ... | 1995 | 8522459 |
| comparison of the nucleotide sequences of the meta-cleavage pathway genes of tol plasmid pww0 from pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution. | tol plasmid pww0 from pseudomonas putida mt-2 encodes catabolic enzymes required for the oxidation of toluene and xylenes. the structural genes for these catabolic enzymes are clustered into two operons, the xylcmabn operon, which encodes a set of enzymes required for the transformation of toluene/xylenes to benzoate/toluates, and the xylxyzltegfjqkih operon, which encodes a set of enzymes required for the transformation of benzoate/toluates to krebs cycle intermediates. the latter operon can be ... | 1993 | 8510667 |
| acinetobacter, an infrequent cause of community acquired bacterial meningitis. | a 19-year-old previously healthy woman developed meningitis due to acinetobacter calcoaceticus. this is a gram-negative coccobacillus belonging to the family neisseriaceae. most reported cases of acinetobacter meningitis were hospital-acquired and due to strains highly resistant to amoxycillin. our patient was treated with amoxycillin and recovered without sequelae. | 1993 | 8453820 |
| precise deletions in large bacterial genomes by vector-mediated excision (vex). the trfa gene of promiscuous plasmid rk2 is essential for replication in several gram-negative hosts. | we have developed a simple and efficient method of vector-mediated excision (vex) for in vivo generation of precisely defined deletions in large bacterial genomes. the system uses homologous recombination with small cloned fragments on specialized pvex plasmids to insert directly repeated bacteriophage p1 loxp sites at positions flanking the region to be deleted. in the presence of cre recombinase, the loxp sites are efficiently recombined to produce the deletion. deletion endpoints can be direc ... | 1993 | 8450534 |
| evolutionary divergence of poba, the structural gene encoding p-hydroxybenzoate hydroxylase in an acinetobacter calcoaceticus strain well-suited for genetic analysis. | the poba gene encoding p-hydroxybenzoate hydroxylase (poba) from acinetobacter calcoaceticus has been developed as a genetic tool for the analysis of structure-function relationships in this enzyme. by exploiting the favorable genetic system of a. calcoaceticus strain adp1, it is possible both to select and to map mutations which disturb poba activity; characterization and sequence determination of mutants derived in this manner may complement site-directed studies with the homologous pseudomona ... | 1993 | 8449410 |
| microbial contamination of enteral feeding solution and its prevention. | in an investigation of microbial contamination of enteral feeding solutions, all 22 residual solutions obtained immediately after administration were contaminated at concentrations of 10(3) to 10(6) viable counts/ml. major contaminants were glucose-nonfermenting gram-negative bacilli such as pseudomonas aeruginosa and acinetobacter calcoaceticus var anitratus. contamination seemed to have been caused by frequent reuse of bag-type containers and the infusion tubes connected to the bags, neither o ... | 1993 | 8442520 |
| physiological characterization of natural transformation in acinetobacter calcoaceticus. | acinetobacter calcoaceticus bd413 develops competence for natural transformation immediately after the start of the exponential growth-phase and remains competent up to e few hours into the stationary phase, after which competence gradually declines. the transformation frequencies obtained strongly depend on the kind of transforming dna and the incubation time with dna. up to 25% of the cells in a culture can be transformed. dna uptake in acinetobacter does not display sequence specificity, is m ... | 1993 | 8436948 |
| effect of sulbactam on infections caused by imipenem-resistant acinetobacter calcoaceticus biotype anitratus. | a recent outbreak of multiresistant strains of acinetobacter calcoaceticus biotype anitratus was observed mostly, but not exclusively, in the surgical intensive care unit in our hospital. disk diffusion and microdilution susceptibility studies demonstrated resistance to imipenem, all aminoglycosides, and all individual beta-lactam antibiotics. only ampicillin plus sulbactam, cefoperazone plus sulbactam, and polymyxin produced zone sizes and mics in the susceptible ranges. determination of mics a ... | 1993 | 8421178 |
| crystallization and preliminary crystallographic investigations of the soluble glucose dehydrogenase from acinetobacter calcoaceticus. | single crystals of the soluble glucose dehydrogenase (gdh) from acinetobacter calcoaceticus have been grown by the vapour diffusion method. these crystals diffract to beyond 2.1 a and are suitable for x-ray crystallography. the space group was determined to be p2(1) with unit cell parameters a = 55.5 a, b = 104.5 a, c = 86.5 a and beta = 99.8 degrees. one asymmetric unit contains a dimer of the gdh molecule. | 1993 | 8411180 |
| [community-acquired meningitis caused by acinetobacter calcoaceticus var. lwoffi in a healthy host]. | 1993 | 8399482 | |
| transposon mutagenesis in acinetobacter calcoaceticus rag-1. | molecular genetic studies of acinetobacter spp. have been greatly limited by the lack of a method for transposon mutagenesis. in this study, a genetically engineered derivative of tn10, mini-tn10pttkm, was conjugally transferred in plate matings from escherichia coli sm10[(lambda pir)(plofpttkm)] to acinetobacter calcoaceticus rag-1. transfer frequencies were dependent on mating ratios and varied from 7.9 x 10(-8) to 3.4 x 10(-7) per recipient cell. the 27 lipase-deficient transconjugants which ... | 1993 | 8383667 |
| evaluation of a commercial dna purification system for plasmid analysis of nosocomial bacterial pathogens. | this study evaluated the promega magic minipreps (mm) (promega corporation, madison, wi) dna purification system for use in plasmid analysis of common nosocomial bacterial pathogens. the mm system is a kit that includes lysis solutions and buffers and incorporates a minicolumn of dna binding resin for recovery of plasmid dna. the mm system was used according to the manufacturer's directions to recover plasmids for agarose gel electrophoresis from clinical isolates of acinetobacter calcoaceticus, ... | 1993 | 8379541 |
| resistance to third-generation cephalosporins in barbados. | resistance to third-generation, or extended-spectrum, cephalosporins caused by induction of class i beta-lactamases has been reported rarely from developing countries. seven isolates of cephalosporin-resistant gram-negative rods were recovered recently from urine, burns and ulcers in the queen elizabeth hospital, barbados. the isolates were identified as acinetobacter calcoaceticus (2), citrobacter freundii (1), enterobacter cloacae (1), morganella morganii (1), providencia stuartii (1) and prot ... | 1993 | 8367967 |
| isolation of acinetobacter calcoaceticus from the gastrointestinal tracts of scid mice. | acinetobacter calcoaceticus colonization was observed in the gastrointestinal tracts of c.-b17-scid/scid (scid) mice, while it was not observed in c.b17-scid/+ and c.b17-+/+ mice with normal immunity housed under the same conditions. a. calcoaceticus and other viable enteric bacteria were not isolated from any organs other than gastrointestinal tract in scid mice. the mice colonized with this organism were apparently healthy and no significant visceral lesion was observed. | 1993 | 8366666 |
| microbial purification technique of mineral dressing plants reject waters. | earlier investigations on laboratory and pilot plant scale have shown the resort to microorganisms to be a practicable approach to the problem of purifying mineral dressing plant reject waters from residual flotation reagents and/or metal ions. in spite of the proven effectiveness of this method, one major drawback, namely the pathogenicity of some microorganisms, has so far hampered its application on a commercial scale. a research programme, aimed at developing a microbial reject water purific ... | 1993 | 8357615 |
| synthesis of allyl o-[sodium(alpha-d-glycero-d-talo-2- octulopyranosyl)onate]-(2-->6)-2-acetamido-2-deoxy-beta-d-glucopyranosi de, a core constituent of the lipopolysaccharide from acinetobacter calcoaceticus nctc 10305. | reaction of methyl 2,6-anhydro-2,3-dideoxy-d-manno-2-octenoate 1 with 3-chloroperoxybenzoic acid gave the 2,3-anhydro derivative 2, which was converted into the per-o-acetylated anomeric methyl glycosides of d-glycero-d-galacto-2-octulopyranosylonic acid in good yield. subsequent inversion of the configuration at c-3 and deprotection afforded sodium (methyl beta-d-glycero-d-talo-2-octulopyranosid)onate. alternatively, 2 was transformed into methyl (alpha-d-glycero-d-talo-2- octulopyranosyl bromi ... | 1993 | 8339301 |
| identification of the transcriptional activator pobr and characterization of its role in the expression of poba, the structural gene for p-hydroxybenzoate hydroxylase in acinetobacter calcoaceticus. | we have identified pobr, a gene encoding a transcriptional activator that regulates expression of poba, the structural gene for p-hydroxybenzoate hydroxylase (poba) in acinetobacter calcoaceticus adp1. inducible expression of cloned poba in escherichia coli depended upon the presence of a functional pobr gene, and mutations within pobr prevented poba expression in a. calcoaceticus. a poba-lacz operon fusion was used to demonstrate that poba expression in a. calcoaceticus is enhanced up to 400-fo ... | 1993 | 8331077 |
| [bacteriologic examinations of patients with burns]. | bacteriological studies were performed on 189 patients treated during 1985-1989. 800 samples were tested which were taken from various sites of burn wound (3-5 smears from a patient). surface of burn amounted to 15-86% and degree of burn was iib/iii. in 772 samples, 2073 bacterial strains were found. most frequent were pseudomonas aeruginosa (31%) and staphylococcus aureus (29%). remaining strains consisted chiefly of proteus mirabilis, pseudomonas cepacia, klebsiella pneumoniae, escherichia col ... | 1993 | 8309304 |
| molecular characterization of an aberrant mercury resistance transposable element from an environmental acinetobacter strain. | we present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pklh2 from an environmental bacterium, acinetobacter calcoaceticus, isolated from a mercury mine. the pklh2 mer operon has essentially the same gene organization as that of tn21 and tn501 from clinical bacteria. the pklh2 mer operon nucleotide sequence shows 85.5% identity with the tn501 and 80.9% identity with the tn21 sequences. vestigial sequences have been found at the ends of the pklh2 mer ope ... | 1993 | 8302940 |
| acinetobacter in denmark: ii. molecular studies of the acinetobacter calcoaceticus-acinetobacter baumannii complex. | the acinetobacter calcoaceticus-acinetobacter baumannii complex consists of four closely related "genospecies" or dna groups: dna group 1 (a. calcoaceticus), dna group 2 (a. baumannii), dna 3, and tjernberg & ursing's dna group 13. strains in this complex are so similar phenotypically that it is often impossible to identify them to the dna group level by the use of biochemical tests. twenty-three danish clinical strains from 23 patients phenotypically identified to the a. calcoaceticus-a. bauman ... | 1993 | 8286091 |
| vascular catheter-related bloodstream infection due to acinetobacter johnsonii (formerly acinetobacter calcoaceticus var. lwoffi): report of 13 cases. | although acinetobacter baumannii (formerly acinetobacter calcoaceticus var. anitratus) is known to be an important nosocomial pathogen, the clinical impact of other acinetobacter species is not fully understood. acinetobacter johnsonii (formerly a subset of a. calcoaceticus var. lwoffii) is considered a commensal on human skin, and infections due to this organism have not been reported previously. during a study period of 18 months, however, 13 patients at 6 hospitals developed catheter-related ... | 1993 | 8268343 |
| acinetobacter meningitis. | 10,468 csf samples from cases of meningitis in different age groups were cultured during 1988-1991. acinetobacter calcoaceticus was isolated in 12 (5.6%) of 211 positive cultures. the strain were 100% resistant to ampicillin, cotrimoxazole and tetracycline 50% resistant to cephazolin gentamicin and kanamycin but 100% susceptible to chloramphenicol. | 1993 | 8258473 |
| characterization of acinetobacter haemolyticus aac(6')-ig gene encoding an aminoglycoside 6'-n-acetyltransferase which modifies amikacin. | the amikacin resistance gene acc(6')-ig of acinetobacter haemolyticus bm2685 encoding an aminoglycoside 6'-n-acetyltransferase was characterized. the gene was identified as a coding sequence of 438 bp corresponding to a protein with a calculated mass of 16,522 da. analysis of the deduced amino acid sequence suggested that it was the fourth member of a subfamily of aminoglycoside 6'-n-acetyltransferases. the resistance gene was not transferable either by conjugation to escherichia coli or to acin ... | 1993 | 8257129 |
| the antimicrobial activity in vitro of cefpirome and 6 other beta-lactam antibacterial agents against clinical isolates. | the in vitro activity of cefpirome (cpr) was compared with that of cefazolin (cez), cefotiam (ctm), flomoxef (fmox), cefotaxime (ctx), ceftazidime (caz) and imipenem/cilastatin (ipm/cs) against 384 clinical isolates, for tested by the both microdilution method. the activity of cpr against staphylococcus aureus (mic50, 1 microgram/ml) and enterococcus faecalis (mic50, 8 micrograms/ml) was better than ctx and caz, but slightly less than ipm/cs. cpr and other antibacterial agents were not effect ag ... | 1993 | 8254889 |
| ciprofloxacin as prophylaxis for urinary tract infection: prospective, randomized, cross-over, placebo controlled study in patients with spinal cord lesion. | to evaluate the efficacy of low-dose long-term treatment with ciprofloxacin in the prevention of recurrent urinary tract infections in patients with spinal cord lesions and neurogenic bladder dysfunction, a prospective, randomized, cross-over, placebo-controlled study was performed. the study period was 12 months long, including 6 months of treatment with 100 mg. ciprofloxacin at night and 6 months of placebo treatment. the study was completed by 18 men and 3 women, median age 38 years (range 19 ... | 1994 | 8254783 |
| growth-phase-dependent expression of the lipolytic system of acinetobacter calcoaceticus bd413: cloning of a gene encoding one of the esterases. | acinetobacter calcoaceticus bd413, when grown in batch culture in nutrient broth, produces both extracellular lipase activity and cell-bound esterase activity during and after the transition between exponential growth and the stationary phase. from a library of a. calcoaceticus dna in escherichia coli, plasmids were isolated that enabled e. coli to grow on media with tributyrin as the sole carbon source. assays with model substrates classified the product of the cloned gene as an esterase. via d ... | 1993 | 8254303 |
| infections caused by imipenem-resistant acinetobacter calcoaceticus biotype anitratus. | 1993 | 8245560 | |
| numerical classification and identification of acinetobacter genomic species. | a total of 211 acinetobacter strains (representing all currently recognized genomic species) were tested for 329 biochemical characters. overall similarities of all strains were determined for 145 characters by numerical taxonomic techniques, the upgma algorithm and the s(sm)) and the s(j) coefficients as measures of similarity. seven clusters (two or more strains) and three unclustered strains were recovered at a similarity level of 80.0% (s(sm). at this level a complete correspondence between ... | 1993 | 8244904 |
| an outbreak of gram-negative bacteremia traced to contaminated o-rings in reprocessed dialyzers. | to investigate an outbreak of gram-negative bacteremia in an outpatient hemodialysis unit and to identify the source of contaminating bacteria and the route by which bacteria gained access to the bloodstream. | 1993 | 8239225 |
| periplasmic aminopeptidases in acinetobacter calcoaceticus and pseudomonas aeruginosa. | the greater part of the intracellular aminopeptidases in pseudomonas aeruginosa and acinetobacter calcoaceticus is soluble. the localization of aminopeptidases in the cells was examined using the osmotic shock method with some modifications. when the cells of a. calcoaceticus and p. aeruginosa of the logarithmic phase were subjected to an osmotic shock, all aminopeptidases investigated were mainly localized in the sucrose supernatants and in the periplasm. acid phosphatase as marker enzyme for p ... | 1993 | 8229672 |
| s-adenosylhomocysteine hydrolase from acinetobacter calcoaceticus: purification and partial characterization. | s-adenosylhomocysteine hydrolase (sahase) was purified to homogeneity from the gram negative strain acinetobacter calcoaceticus 501. the molecular weight of the native enzyme, estimated by gel permeation, was about 288 kda, while sodium dodecyl sulfate polyacrylamide gel electrophoresis yielded a relative molecular mass of 48 kda. the determination of the coenzyme content gave 4 mol of nad+ and 2 mol of nadh per mol of enzyme. the isoelectric point of native sahase was at ph 5.1. when assayed in ... | 1993 | 8220254 |
| isolation, characterization, and sequence analysis of cryptic plasmids from acinetobacter calcoaceticus and their use in the construction of escherichia coli shuttle plasmids. | three cryptic plasmids have been discovered in acinetobacter calcoaceticus bd413. these three plasmids, designated pwm10 (7.4 kb), pwm11 (2.4 kb), and pwm12 (2.2 kb), exhibited extensive homology to one another, as shown by southern blot hybridization and restriction site analysis data, and also hybridized with three plasmids having slightly different sizes detected in a second strain, a. calcoaceticus bd4. plasmid pwm11 and a fragment of pwm10 were each subcloned into puc19, yielding plasmids p ... | 1993 | 8215356 |
| rpon (sigma 54) is required for conversion of phenol to catechol in acinetobacter calcoaceticus. | members of the sigma 54 protein family, encoded by rpon, are required for the transcription of genes associated with specialized metabolic functions. the ability to grow with phenol appears to be a specialized trait because it is expressed by few of the microorganisms that grow with catechol, the metabolic product of phenol monooxygenase. a mutation preventing the expression of phenol monooxygenase in the bacterial strain acinetobacter calcoaceticus ncib8250 was complemented by wild-type dna seg ... | 1994 | 8206826 |