Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| bacterial cholangitis in patients with biliary atresia: impact on short-term outcome. | bacterial cholangitis (bc) is a common complication in patients with biliary atresia (ba) and is characterized by fever, acholic stools and positive blood cultures. the diagnosis is often empirical because the yield of blood cultures is low. it is difficult to differentiate bc from other febrile episodes. in order to characterize the clinical and laboratory features of bc in patients with ba, identify risk factors, and correlate cholangitis with outcome, 37 patients with ba from 1993 to 1998 who ... | 2001 | 11527173 |
| risk factors for acinetobacter baumannii nosocomial bacteremia in critically ill patients: a cohort study. | nosocomial bacteremia caused by acinetobacter baumannii (ab) is of increasing concern in critically ill patients, and the risk factors for this infection are not well established. an inception cohort study in a 40-bed medical and surgical intensive care unit (icu) at a single institution was conducted during a 2-year period to determine the risk factors for ab nosocomial bacteremia. risk factors related to the underlying diseases, the clinical picture at admission, and those acquired during the ... | 2001 | 11528563 |
| plasmid-encoded metallo-beta-lactamase (imp-6) conferring resistance to carbapenems, especially meropenem. | in 1996, serratia marcescens ku3838 was isolated from the urine of a patient with a urinary tract infection at a hospital in northern japan and was found to contain the plasmid pku501. previously, we determined that pku501 carries bla(imp) and the genes for tem-1-type beta-lactamases as well as producing both types of beta-lactamases (h. yano, a. kuga, k. irinoda, r. okamoto, t. kobayashi, and m. inoue, j. antibiot. 52:1135-1139, 1999). pku502 is a recombinant plasmid that contains a 1.5-kb dna ... | 2001 | 11302793 |
| risk factors for nosocomial pneumonia in critically ill trauma patients. | to determine risk factors for nosocomial pneumonia in critically ill trauma patients. | 2001 | 11246310 |
| sulbactam efficacy in experimental models caused by susceptible and intermediate acinetobacter baumannii strains. | sulbactam and imipenem were compared in an experimental pneumonia model in immunocompetent mice, using a susceptible strain of acinetobacter baumannii, and in an experimental endocarditis model in rabbits, using an intermediately susceptible strain. in the former, sulbactam was as efficacious as imipenem in terms of survival, sterility of lungs and in the bacterial clearance from lungs and blood, provided that the t > mic for sulbactam (1.84 h) was similar to that for imipenem (2.01 h). in the e ... | 2001 | 11266426 |
| acinetobacter baumanii (ab) bacteraemia in surgical patients. | 2001 | 11560397 | |
| cloning and genetic characterization of dca genes required for beta-oxidation of straight-chain dicarboxylic acids in acinetobacter sp. strain adp1. | a previous study of deletions in the protocatechuate (pca) region of the acinetobacter sp. strain adp1 chromosome revealed that genes required for utilization of the six-carbon dicarboxylic acid, adipic acid, are linked to the pca structural genes. to investigate the genes involved in adipate catabolism, a 33.8-kb saci fragment, which corrects a deletion spanning this region, was cloned. in addition to containing known pca, qui, and pob genes (for protocatechuate, quinate, and 4-hydroxybenzoate ... | 2001 | 11571189 |
| molecular identification of campylobacter concisus. | a 1.6-kb dna fragment isolated from a campylobacter concisus genomic library gave c. concisus-specific restriction fragment length patterns when it was used as a probe in hybridization studies. all of the strains tested, including type strains and clinical isolates, contained a 0.5-kb hindiii fragment that hybridized to the probe. dna sequencing of the 1.6-kb fragment identified three open reading frames (orfs). one of the orfs encodes the carboxy terminus of gyrb, and the translational products ... | 2001 | 11574591 |
| clinical significance and taxonomy of actinobacillus hominis. | clinical findings in 36 immunosuppressed patients with lower respiratory tract infection or bacteremia with actinobacillus hominis are described. animal contact was only recorded for three patients; nine patients died despite appropriate antimicrobial treatment. although infections with this microorganism seem to be rare, the fact that 37 of 46 strains characterized in this study have been found in copenhagen indicates that under-reporting may occur. a. hominis is phenotypically relatively homog ... | 2001 | 11230406 |
| outbreak of acinetobacter baumannii in an intensive care unit associated with tracheostomy. | a case-control study was conducted to determine risk factors for acquisition of an epidemic strain of acinetobacter baumannii in an intensive care unit. the epidemic strain was identified by pulsed-field gel electrophoresis and was isolated from tracheal secretions in 13 (87%) of 15 patients. in a logistic regression analysis, presence of a tracheostomy was an independent risk for acinetobacter sp acquisition (odds ratio, 421; 95% confidence interval, 13.8-12925; p =.001) and the strength of the ... | 2001 | 11584252 |
| periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment. | periodontal disease is perhaps the most common chronic infection in adults. evidence has been accumulating for the past 30 years which indicates that almost all forms of periodontal disease are chronic but specific bacterial infections due to the overgrowth in the dental plaque of a finite number of mostly anaerobic species such as porphyromonas gingivalis, bacteroides forsythus, and treponema denticola. the success of traditional debridement procedures and/or antimicrobial agents in improving p ... | 2001 | 11585783 |
| molecular detection of antimicrobial resistance. | the determination of antimicrobial susceptibility of a clinical isolate, especially with increasing resistance, is often crucial for the optimal antimicrobial therapy of infected patients. nucleic acid-based assays for the detection of resistance may offer advantages over phenotypic assays. examples are the detection of the methicillin resistance-encoding meca gene in staphylococci, rifampin resistance in mycobacterium tuberculosis, and the spread of resistance determinants across the globe. how ... | 2001 | 11585788 |
| extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. | beta-lactamases continue to be the leading cause of resistance to beta-lactam antibiotics among gram-negative bacteria. in recent years there has been an increased incidence and prevalence of extended-spectrum beta-lactamases (esbls), enzymes that hydrolyze and cause resistance to oxyimino-cephalosporins and aztreonam. the majority of esbls are derived from the widespread broad-spectrum beta-lactamases tem-1 and shv-1. there are also new families of esbls, including the ctx-m and oxa-type enzyme ... | 2001 | 11585791 |
| characterization of listeria monocytogenes strains involved in invasive and noninvasive listeriosis outbreaks by pcr-based fingerprinting techniques. | a total of 32 listeria monocytogenes strains (16 from a recent outbreak of invasive listeriosis and 16 from two outbreaks of noninvasive listeriosis, all three occurring in italy) were characterized by pcr-ribotyping, arbitrarily primed pcr (ap-pcr), and the recently developed infrequent-restriction-site pcr (irs-pcr). the discriminatory ability of the techniques, first evaluated on 29 unrelated l. monocytogenes food isolates using simpson's index of diversity, was 0.714 for pcr-ribotyping, 0.69 ... | 2001 | 11282635 |
| imp-4, a novel metallo-beta-lactamase from nosocomial acinetobacter spp. collected in hong kong between 1994 and 1998. | between 1994 and 1998, 97 imipenem-resistant acinetobacter isolates were identified at the prince of wales hospital, hong kong, china. a bla(imp) pcr product was obtained from 23 of 35 viable cultures; 12 isolates belonged to genomic dna group 3, 8 belonged to group 2 (acinetobacter baumannii), 2 belonged to group 13tu, and 1 belonged to group 1. the bla(imp) homologues were sequenced from two isolates from genomic dna group 2 and one isolate each from groups 3 and 13tu. the four sequences inclu ... | 2001 | 11181348 |
| bacteremia in hospitalized patients with human immunodeficiency virus: a prospective, cohort study. | bacterial infections complicate the course of patients with human immunodeficiency virus infection. the purpose of this study was to describe the bacterial pathogens causing blood stream infection, identify the risk factors for the development of blood stream infection and determine the impact of blood stream infection on the outcome of patients infected with human immunodeficiency virus. | 2001 | 11602019 |
| in vitro activity of moxifloxacin against common clinical bacterial isolates in taiwan. | the in vitro antimicrobial activities of moxifloxacin were compared with 7 other antimicrobial agents. a total of 707 isolates of 11 common pathogenic bacteria were collected from the national taiwan university hospital; antimicrobial activities against these isolates were evaluated by minimum inhibitory concentration using an agar-dilution method. most common pathogenic bacteria were susceptible to moxifloxacin, including methicillin-susceptible and -resistant staphylococcus aureus, methicillin ... | 2001 | 11605808 |
| acinetobacter baumanii (ab) bacteremia in cancer patients. | 2001 | 11680838 | |
| identification and antimicrobial susceptibility of alcaligenes xylosoxidans isolated from patients with cystic fibrosis. | in the past decade, potential pathogens, including alcaligenes species, have been increasingly recovered from cystic fibrosis (cf) patients. accurate identification of multiply antibiotic-resistant gram-negative bacilli is critical to understanding the epidemiology and clinical implications of emerging pathogens in cf. we examined the frequency of correct identification of alcaligenes spp. by microbiology laboratories affiliated with american cf patient care centers. selective media, an exotoxin ... | 2001 | 11682511 |
| biochemical characterization of chromosomal cephalosporinases from isolates belonging to the acinetobacter baumannii complex. | 2001 | 11422246 | |
| generation and serological characterization of murine monoclonal antibodies against o antigens from acinetobacter reference strains. | o-antigen-specific monoclonal antibodies were generated against acinetobacter strains from international type culture collections and characterized by enzyme immunoassay and western and colony blotting. the antibodies aid in the further completion of an o-serotyping scheme for acinetobacter and, due to their high specificity, are especially useful to all working with these strains. | 2001 | 11427435 |
| groel encodes a highly antigenic protein in burkholderia pseudomallei. | no recombinant protein is available for serodiagnosis of melioidosis. in this study, we report the cloning of the groel gene, which encodes an immunogenic protein of burkholderia pseudomallei. bidirectional dna sequencing of groel revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kda. basic local alignment search tool analysis showed that the putative protein encoded by groel is homologous to the chaperonins enco ... | 2001 | 11427437 |
| development of a pcr assay for identification of staphylococci at genus and species levels. | we have developed a pcr-based assay which allows the detection of staphylococci at the genus level by targeting the tuf gene, which encodes the elongation factor tu. degenerate pcr primers derived from consensus regions of several tuf genes were used to amplify a target region of 884 bp from 11 representative staphylococcal species. subsequently, the entire nucleotide sequence of these amplicons was determined. the analysis of a multiple alignment of these sequences revealed regions conserved am ... | 2001 | 11427566 |
| oxa-28, an extended-spectrum variant of oxa-10 beta-lactamase from pseudomonas aeruginosa and its plasmid- and integron-located gene. | pseudomonas aeruginosa ed-1, isolated from a pulmonary brush of a patient hospitalized in a suburb of paris, france, was resistant to ceftazidime and of intermediate susceptibility to ureidopenicillins and to cefotaxime. cloning and expression of the beta-lactamase gene content of this isolate in escherichia coli dh10b identified a novel oxa-10 variant, oxa-28, with a pi value of 8.1 and a molecular mass of 29 kda. it differed from oxa-10 by 10 amino acid changes and from oxa-13 and oxa-19 by 2 ... | 2001 | 11158739 |
| antibiotic resistance is a major risk factor for epidemic behavior of acinetobacter baumannii. | to study the presence of bacterial factors in clinical isolates of acinetobacter species in order to identify markers of epidemic potential. | 2001 | 11428438 |
| investigation of an outbreak of multi-drug resistant acinetobacter baumannii in an intensive care burns unit. | over a three month period there was an outbreak of infection, due to a multi-drug resistant acinetobacter baumannii in the intensive care burns unit with spread of infection to other patients, both within the unit and elsewhere in the hospital. microbiological sampling of the environment and of the healthcare workers' (hcws) hands were carried out. strain relatedness of the isolates was confirmed by pulsed field gel electrophoresis. fifteen patients were involved in the outbreak, whose infection ... | 2001 | 11439011 |
| characterization of class 1 integrons from pseudomonas aeruginosa that contain the bla(vim-2) carbapenem-hydrolyzing beta-lactamase gene and of two novel aminoglycoside resistance gene cassettes. | two clonally unrelated pseudomonas aeruginosa clinical strains, ron-1 and ron-2, were isolated in 1997 and 1998 from patients hospitalized in a suburb of paris, france. both isolates expressed the class b carbapenem-hydrolyzing beta-lactamase vim-2 previously identified in marseilles in the french riviera. in both isolates, the bla(vim-2) cassette was part of a class 1 integron that also encoded aminoglycoside-modifying enzymes. in one case, two novel aminoglycoside resistance gene cassettes, aa ... | 2001 | 11158753 |
| antimicrobial resistance surveillance of bacteria in 1999 in korea with a special reference to resistance of enterococci to vancomycin and gram-negative bacilli to third generation cephalosporin, imipenem, and fluoroquinolone. | the trend of antimicrobial resistance of bacteria isolated from patients in 30 korean hospitals in 1999 was analyzed with a particular attention to cefotaxime- or fluoroquinolone-resistant gram-negative bacilli, imipenem-resistant pseudomonas aeruginosa, and vancomycin-resistant enterococci. adequacy of susceptibility testing, and any change in the frequencies of isolated species were also analyzed. the results showed that only 20% and 30% of hospitals tested the piperacillin-tazobactam and cefo ... | 2001 | 11447969 |
| identification and characterization of a novel family of pneumococcal proteins that are protective against sepsis. | four pneumococcal genes (phta, phtb, phtd, and phte) encoding a novel family of homologous proteins (32 to 87% identity) were identified from the streptococcus pneumoniae genomic sequence. these open reading frames were selected as potential vaccine candidates based upon their possession of hydrophobic leader sequences which presumably target these proteins to the bacterial cell surface. analysis of the deduced amino acid sequences of these gene products revealed the presence of a histidine tria ... | 2001 | 11159990 |
| microbiologic aspects of predominant bacteria isolated from the burn patients in korea. | the risk of infection in burns is well-known. in recent decades, the antimicrobial resistance of bacteria isolated from burn patients has increased. for this reason, we have carried out a study of the predominant bacterial profiles and antimicrobial resistance patterns of isolates from a burn center in korea. a retrospective study was undertaken at hallym university, hangang sacred heart hospital to examine the bacterial isolates from the burn patients and to compare the antibiograms of the pred ... | 2001 | 11226650 |
| novel class a beta-lactamase sed-1 from citrobacter sedlakii: genetic diversity of beta-lactamases within the citrobacter genus. | citrobacter sedlakii 2596, a clinical strain resistant to aminopenicillins, carboxypenicillins, and early cephalosporins such as cephalothin, but remaining susceptible to acylureidopenicillins, carbapenems, and later cephalosporins such as cefotaxime, was isolated from the bile of a patient treated with beta-lactam and quinolone antibiotics. the isolate produced an inducible class a beta-lactamase of pi 8.6, named sed-1, which was purified. characterized by a molecular mass of 30 kda, sed-1 pref ... | 2001 | 11451687 |
| mutation in serratia marcescens ampc beta-lactamase producing high-level resistance to ceftazidime and cefpirome. | starting from a clinical isolate of serratia marcescens that produced a chromosomally encoded ampc beta-lactamase inducibly, we isolated by stepwise selection two laboratory mutants that showed high levels of resistance to some cephalosporins. the 98r mutant apparently overproduced the unaltered beta-lactamase constitutively, but the 520r mutant produced an altered enzyme, also constitutively. ceftazidime and cefpirome mics for the 520r mutant were much higher (512 and 64 microg/ml, respectively ... | 2001 | 11451693 |
| nosocomial pneumonia: importance of recognition of aetiological agents to define an appropriate initial empirical therapy. | of the 16,024 patients hospitalized from january 1995 to october 1997, 397 (2.4%) acquired nosocomial pneumonia and the aetiological agent was defined in 101 (25%). about 82% developed late onset pneumonia (>7 days of hospitalization). the site of isolation of microorganisms was bronchoalveolar lavage (bal) (49%), blood culture (39%), pleural effusion (10%) and (2%) pulmonary tissue. gram-negative bacteria were responsible for 54% of infections. staphylococcus aureus (34%) was the most frequent ... | 2001 | 11165120 |
| in vitro activities of abt-773, a new ketolide, against aerobic and anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis. | the comparative in vitro activities of abt-773 against 207 aerobic and 162 anaerobic antral sinus puncture isolates showed that erythromycin-resistant pneumococcal strains were susceptible to abt-773 (< or =0.125 microg/ml); the mic at which 90% of the isolates tested were inhibited for haemophilus influenzae and other haemophilus spp. was 4 microg/ml; and all moraxella spp. and beta-lactamase-producing prevotella species strains were inhibited by < or =0.125 microg/ml. among the anaerobes teste ... | 2001 | 11451698 |
| identification of a plasmid encoding shv-12, tem-1, and a variant of imp-2 metallo-beta-lactamase, imp-8, from a clinical isolate of klebsiella pneumoniae. | a multidrug-resistant plasmid encoding tem-1, shv-12, and a variant of imp-2 metallo-beta-lactamase, designated imp-8, was identified from a clinical isolate of klebsiella pneumoniae. there are four nucleotide differences between bla(imp-2) and bla(imp-8), resulting in two amino acid differences. bla(imp-8) was also found to be carried by an integron-borne gene cassette similar to the bla(imp-2) cassette. | 2001 | 11451699 |
| [the effect of tannery waste products on viability of selected bacteria from activated sludge]. | in this study we have investigated the influence of sludge on the activity of bacteria strains: acinetobacter baumannii, aeromonas hydrophila and pseudomonas cepacia. to our studies we have taken waste materials used in the chromium compound leather tanning process as well as materials from leather fat and waste materials, in general. it was confirmed that 1:20 dilution of chromium tanning leather waste, retanage and leather fat allowed the survivability of bacteria in all three strains. through ... | 2001 | 11452739 |
| acinetobacter baumannii--an emerging nosocomial pathogen in the burns unit manipal, india. | a retrospective study on 113 patients admitted to the burns unit over a period of 12 months was undertaken to investigate the emerging trend of acinetobacter baumannii infection. wound infection with a. baumannii was seen in 13 (11.5%) patients. of these six (46%) patients developed septicaemia with the same bacteria and five (38%) expired. the virulence of these bacteria, drug susceptibility pattern and its role as a nosocomial pathogen is discussed. | 2001 | 11226651 |
| efficacy of cefepime versus ceftazidime in the treatment of adult pneumonia. | effective empiric treatment of pneumonia requires antibiotic coverage against gram-negative and gram-positive pathogens, including drug-resistant isolates. this study evaluated the efficacy of cefepime treatment in 20 patients with community-acquired pneumonia (cap) and 21 patients with hospital-acquired pneumonia (hap), and ceftazidime treatment in 20 patients with hap. the mean age of patients was over 70 years. more than half of the patients had multiple lobe involvement. there was no signifi ... | 2001 | 11456359 |
| typing of acinetobacter baumannii isolated from hospital-acquired respiratory infections in a tertiary care centre in southern india. | in an attempt to define the epidemiology of acinetobacter baumannii infection, 27 isolates, obtained from hospital-acquired respiratory infections, were typed using random amplified polymorphic dna (rapd) profile and antimicrobial susceptibility patterns. ten different patterns were obtained with eric2 primer: 14 isolates had a similar profile representing a single strain. within rapd types, isolates could be further classified based on their antibiogram; however, strains of different types had ... | 2001 | 11170782 |
| evaluation of vitek 2 rapid identification and susceptibility testing system against gram-negative clinical isolates. | a total of 281 strains of miscellaneous members of the family enterobacteriaceae, pseudomonas aeruginosa, and other gram-negative bacteria were evaluated by use of identification tests with the vitek 2 system (biomérieux) and an api identification system (biomérieux). a total of 237 (95%) strains were correctly identified to the species level. only six (2.1%) strains were misidentified, and eight (2.8%) strains were not identified. among 14 strains with discrepant identifications, 8 (57.1%) stra ... | 2001 | 11474023 |
| evaluation of current activities of fluoroquinolones against gram-negative bacilli using centralized in vitro testing and electronic surveillance. | given the propensity for enterobacteriaceae and clinically significant nonfermentative gram-negative bacilli to acquire antimicrobial resistance, consistent surveillance of the activities of agents commonly prescribed to treat infections arising from these organisms is imperative. this study determined the activities of two fluoroquinolones, levofloxacin and ciprofloxacin, and seven comparative agents against recent clinical isolates of enterobacteriaceae, pseudomonas aeruginosa, acinetobacter b ... | 2001 | 11120976 |
| characterization of oxa-25, oxa-26, and oxa-27, molecular class d beta-lactamases associated with carbapenem resistance in clinical isolates of acinetobacter baumannii. | carbapenem resistance in acinetobacter spp. is increasingly being associated with oxa-type beta-lactamases with weak hydrolytic activity against imipenem and meropenem. such enzymes were characterized from acinetobacter isolates collected in belgium, kuwait, singapore, and spain. the isolates from spain and belgium had novel class d beta-lactamases that were active against carbapenems. these were designated oxa-25 and oxa-26, respectively, and had >98% amino acid homology with each other and wit ... | 2001 | 11158758 |
| identification of epidemic strains of acinetobacter baumannii by integrase gene pcr. | forty-eight clinical acinetobacter isolates with different epidemic behavior were investigated for the presence of integrons and plasmids and for antibiotic susceptibility. integrons were demonstrated in 50% of the strains by an integrase gene pcr. epidemic strains of acinetobacter baumannii were found to contain significantly more integrons than nonepidemic strains. also, the presence of integrons was significantly correlated with simultaneous resistance to several antibiotics. plasmids were de ... | 2001 | 11136740 |
| contemporary assessment of antimicrobial susceptibility testing methods for polymyxin b and colistin: review of available interpretative criteria and quality control guidelines. | the emergence of infections caused by multidrug-resistant pseudomonas aeruginosa and acinetobacter spp. has necessitated the search for alternative parenteral agents such as the polymyxins. the national committee for clinical laboratory standards (nccls) documents do not currently provide interpretative criteria for the testing of the polymyxins, colistin and polymyxin b. therefore, an evaluation of the antimicrobial activity of colistin and polymyxin b was initiated using 200 bloodstream infect ... | 2001 | 11136768 |
| epidemiology and infection control implications of acinetobacter spp. in hong kong. | in a previous study, we showed that acinetobacter genomic dna group 3 was the most common species among blood culture isolates and was commonly found on superficial carriage sites of the healthy and the sick, which are different findings from those reported in europe and north america. we used amplified ribosomal dna restriction analysis and pulsed-field gel electrophoresis to study further the molecular epidemiology of acinetobacters in our region. over a study period of 6 weeks with 136 consec ... | 2001 | 11136776 |
| acinetobacter baumannii at a tertiary-care teaching hospital in jerusalem, israel. | in a retrospective 10-year analysis of 3,536 patient-unique isolates, acinetobacter baumannii imipenem susceptibility declined from 98.1 (1990) to 64.1% (2000), and ciprofloxacin susceptibility decreased from 50.5 to 13.1%. imipenem median zone diameters decreased from 27. 7 (1997) to 18.8 mm (2000). no outbreaks were detected. two clusters were identified for 41 strains genotyped by pulsed-field gel electrophoresis, but imipenem resistance was not clonal. | 2001 | 11136809 |
| ceftibuten stability to active-site serine and metallo-beta-lactamases. | ceftibuten is an oral third-generation cephalosporin active against a wide range of bacteria and shows an improved stability to hydrolysis by several beta-lactamases because of the carboxyethilidine moiety at position 7 of the ss-acyl side chain. the kinetic interactions between ceftibuten and active-site serine and metallo-ss-lactamases were investigated. the activity of several tem-derived extended spectrum beta-lactamases (esbetals) against ceftibuten, cefotaxime and ceftazidime was compared ... | 2001 | 11137648 |
| [activity of cefepime compared with other antibiotics against major hospital bacterial pathogens]. | the aim of the study was to evaluate susceptibility of 976 bacterial isolates from nosocomial infections to cefepime and 9 other antibiotics used in the treatment of hospitalised patients. bacterial strains were mainly derived from wound and soft tissue infections, sputum, abscesses and blood. the most prevalent etiologic agents were: pseudomonas aeruginosa (18.7%), escherichia coli (13.8%), staphyloccocus aureus (9.7%, acinetobacter baumannii (9.12%), klebsiella pneumoniae (7.38%), enterobacter ... | 2001 | 11320582 |
| transferable antibiotic resistance in multiresistant nosocomial acinetobacter baumannii strains from seven clinics in the slovak and czech republics. | sixty-seven multiresistant nosocomial acinetobacter baumannii isolates from patients hospitalized mostly in intensive care units of seven clinics in slovak and czech republic were tested to determine their ability to transfer antibiotic resistance. all isolates were resistant to kanamycin, ticarcillin, cephalothin, cefotaxime, ceftazidime, aztreonam and susceptible to carbapenems, sulbactam and ampicillin/sulbactam. sixty-five out of 67 strains transferred resistance determinants to escherichia ... | 2001 | 11330360 |
| identification and functional characterization of arylamine n-acetyltransferases in eubacteria: evidence for highly selective acetylation of 5-aminosalicylic acid. | arylamine n-acetyltransferase activity has been described in various bacterial species. bacterial n-acetyltransferases, including those from bacteria of the gut flora, may be involved in the metabolism of xenobiotics, thereby exerting physiopathological effects. we characterized these enzymes further by steady-state kinetics, time-dependent inhibition, and dna hybridization in 40 species, mostly from the human intestinal microflora. we report for the first time n-acetyltransferase activity in 11 ... | 2001 | 11344150 |
| quinolone resistance-determining regions of gyra and parc in pasteurella multocida strains with different levels of nalidixic acid resistance. | 2001 | 11270370 | |
| genetic organization of the region encoding regulation, biosynthesis, and transport of rhizobactin 1021, a siderophore produced by sinorhizobium meliloti. | eight genes have been identified that function in the regulation, biosynthesis, and transport of rhizobactin 1021, a hydroxamate siderophore produced under iron stress by sinorhizobium meliloti. the genes were sequenced, and transposon insertion mutants were constructed for phenotypic analysis. six of the genes, named rhbabcdef, function in the biosynthesis of the siderophore and were shown to constitute an operon that is repressed under iron-replete conditions. another gene in the cluster, name ... | 2001 | 11274118 |
| evaluation of inoculum addition to stimulate in situ bioremediation of oily-sludge-contaminated soil. | a full-scale study evaluating an inoculum addition to stimulate in situ bioremediation of oily-sludge-contaminated soil was conducted at an oil refinery where the indigenous population of hydrocarbon-degrading bacteria in the soil was very low (10(3) to 10(4) cfu/g of soil). a feasibility study was conducted prior to the full-scale bioremediation study. in this feasibility study, out of six treatments, the application of a bacterial consortium and nutrients resulted in maximum biodegradation of ... | 2001 | 11282620 |
| epidemiological characterization of hospital-acquired acinetobacter baumannii isolates from a 1500-bed teaching hospital by phenotypic and genotypic methods. | 2001 | 11247688 | |
| biochemical characterization of the fez-1 metallo-beta-lactamase of legionella gormanii atcc 33297t produced in escherichia coli. | the bla(fez-1) gene coding for the metallo-beta-lactamase of legionella (fluoribacter) gormanii atcc 33297t was overexpressed via a t7 expression system in escherichia coli bl21(de3)(plyss). the product was purified to homogeneity in two steps with a yield of 53%. the fez-1 metallo-beta-lactamase exhibited a broad-spectrum activity profile, with a preference for cephalosporins such as cephalothin, cefuroxime, and cefotaxime. monobactams were not hydrolyzed. the beta-lactamase was inhibited by me ... | 2001 | 11257043 |
| [detection of gram-negative bacteria in patients and hospital environment at a room in geriatric wards under the infection control against mrsa]. | we prospectively surveyed gram-negative bacteria in patients and hospital environment in a room in the geriatric ward which was specifically under the infection control against mrsa once every two weeks between september and december 1996. we investigated the inpatients in an 8-bed room in the geriatric wards (190 beds) of aino memorial hospital, affiliated with nagasaki university. during the study period, we performed a total of 431 cultures. the number of specimens cultured was 116 from airwa ... | 2001 | 11260881 |
| controlled clinical comparison of bactec plus anaerobic/f to standard anaerobic/f as the anaerobic companion bottle to plus aerobic/f medium for culturing blood from adults. | to determine the optimal anaerobic companion bottle to pair with bactec plus aerobic/f medium for recovery of pathogenic microorganisms from adult patients with bacteremia and fungemia, we compared plus anaerobic/f bottles with standard anaerobic/f bottles, each of which was filled with 4 to 6 ml of blood. the two bottles were paired with a plus aerobic/f bottle filled with 8 to 12 ml of blood. a total of 14,011 blood culture sets were obtained. of these, 11,583 sets were received with all three ... | 2001 | 11230415 |
| meropenem in neonatal severe infections due to multiresistant gram-negative bacteria. | recently, new broad spectrum carbapenem has been investigated on a world-wide scale for the treatment of moderate to severe infections. in the neonatal intensive care units the extensive use of third generation cephalosporins for therapy of neonatal sepsis may lead to rapid emergence of multiresistant gram-negative organisms. we report the use of meropenem in 35 infants with severe infections due to acinetobacter baumanii and klebsiella pneumoniae. all gram negative bacteria were resistant to am ... | 2001 | 11237230 |
| analysis of the pmsceab gene cluster involved in biosynthesis of salicylic acid and the siderophore pseudomonine in the biocontrol strain pseudomonas fluorescens wcs374. | mutants of pseudomonas fluorescens wcs374 defective in biosynthesis of the fluorescent siderophore pseudobactin still display siderophore activity, indicating the production of a second siderophore. a recombinant cosmid clone (pmb374-07) of a wcs374 gene library harboring loci necessary for the biosynthesis of salicylic acid (sa) and this second siderophore pseudomonine was isolated. the salicylate biosynthesis region of wcs374 was localized in a 5-kb ecori fragment of pmb374-07. the sa and pseu ... | 2001 | 11222588 |
| effects exerted by transcriptional regulator pcau from acinetobacter sp. strain adp1. | protocatechuate degradation is accomplished in a multistep inducible catabolic pathway in acinetobacter sp. strain adp1. the induction is brought about by the transcriptional regulator pcau in concert with the inducer protocatechuate. pcau, a member of the new iclr family of transcriptional regulators, was shown to play a role in the activation of transcription at the promoter for the structural pca genes, leaving open the participation of additional activators. in this work we show that there i ... | 2001 | 11208784 |
| an outbreak of imipenem-resistant acinetobacter baumannii in critically ill surgical patients. | to describe an outbreak of imipenem-resistant acinetobacter baumannii (ir-ab) and the measures for its control, and to investigate risk factors for ir-ab acquisition. | 2001 | 11198020 |
| a nosocomial outbreak of multiresistant acinetobacter baumannii originating from an intensive care unit. | an outbreak of a multiresistant acinetobacter baumannii in february through september 1996 affected 103 patients in a regional hospital in singapore. we describe the effectiveness of closure of the outbreak area and the importance of good teamwork in the management of the outbreak. | 2001 | 11198024 |
| gene structures and regulation of the alkane hydroxylase complex in acinetobacter sp. strain m-1. | in the long-chain n-alkane degrader acinetobacter sp. strain m-1, two alkane hydroxylase complexes are switched by controlling the expression of two n-alkane hydroxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubredoxin ruductase are encoded by a single gene and expressed constitutively. | 2001 | 11160120 |
| in vitro and in vivo properties of ro 63-9141, a novel broad-spectrum cephalosporin with activity against methicillin-resistant staphylococci. | ro 63-9141 is a new member of the pyrrolidinone-3-ylidenemethyl cephem series of cephalosporins. its antibacterial spectrum was evaluated against significant gram-positive and gram-negative pathogens in comparison with those of reference drugs, including cefotaxime, cefepime, meropenem, and ciprofloxacin. ro 63-9141 showed high antibacterial in vitro activity against gram-positive bacteria except ampicillin-resistant enterococci, particularly vancomycin-resistant strains of enterococcus faecium. ... | 2001 | 11181368 |
| metallo-beta-lactamase producers in environmental microbiota: new molecular class b enzyme in janthinobacterium lividum. | eleven environmental samples from different sources were screened for the presence of metallo-beta-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of edta-inhibitable carbapenemase activity. a total of 15 metallo-beta-lactamase-producing isolates, including 10 stenotrophomonas maltophilia isolates, 3 chryseobacterium spp., one aeromonas hydrophila isolate, and one janthinobacterium lividum ... | 2001 | 11181369 |
| phenotypic resistance of staphylococcus aureus, selected enterobacteriaceae, and pseudomonas aeruginosa after single and multiple in vitro exposures to ciprofloxacin, levofloxacin, and trovafloxacin. | the phenotypic resistance of selected organisms to ciprofloxacin, levofloxacin, and trovafloxacin was defined as a mic of > or =4 microg/ml. the dynamics of resistance were studied after single and sequential drug exposures: clinical isolates of methicillin-susceptible and methicillin-resistant staphylococcus aureus (mssa and mrsa), escherichia coli, klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, and pseudomonas aeruginosa were utilized. after a single 48-h exposure of a large ... | 2001 | 11181375 |
| polymyxin b-resistant acinetobacter baumannii clinical isolate susceptible to recombinant bpi and cecropin p1. | 2001 | 16557680 | |
| antibiotic resistance pattern in uropathogens. | uropathogenic strains from inpatient and outpatient departments were studied from april 1997 to march 1999 for their susceptibility profiles. the various isolates were escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, acinetobacter baumanii and enterococcus faecalis. antibiotic susceptibility pattern of these isolates revealed that for outpatients, first generation cephalosporins, nitrofurantoin, norfloxacin/ciprofloxacin were effective for treatment of urinary ... | 2002 | 17657041 |
| antimicrobial drug resistance in pathogens causing nosocomial infections at a university hospital in taiwan, 1981-1999. | to determine the distribution and antimicrobial drug resistance in bacterial pathogens causing nosocomial infections, surveillance data on nosocomial infections documented from 1981 to 1999 at national taiwan university hospital were analyzed. during this period, 35,580 bacterial pathogens causing nosocomial infections were identified. candida species increased considerably, ranking first by 1999 in the incidence of pathogens causing all nosocomial infections, followed by staphylococcus aureus a ... | 2002 | 11749750 |
| antimicrobial susceptibilities among clinical isolates of extended-spectrum cephalosporin-resistant gram-negative bacteria in a taiwanese university hospital. | infections caused by gram-negative bacteria with resistance to extended-spectrum cephalosporins require the identification of effective alternative antimicrobial therapy. to determine the role of other pre-existing or currently available antimicrobial agents in treating infections caused by these multidrug-resistant pathogens, we evaluated the in vitro susceptibilities of these agents in 411 non-duplicate isolates of extended-spectrum cephalosporin-resistant gram-negative bacteria recovered betw ... | 2002 | 11751769 |
| nosocomial antibiotic resistance in multiple gram-negative species: experience at one hospital with squeezing the resistance balloon at multiple sites. | increased use of antibiotics has led to the isolation of multidrug-resistant bacteria, especially in intensive care units and long-term care facilities. resistance in specific gram-negative bacteria, including klebsiella pneumoniae, acinetobacter baumannii, and pseudomonas aeruginosa, is of great concern, because a growing number of reports have documented mechanisms whereby these microorganisms have become resistant to all available antibacterial agents used in therapy. reduction in the selecti ... | 2002 | 11797177 |
| susceptibility to fluoroquinolones among commonly isolated gram-negative bacilli in 2000: trust and tsn data for the united states. tracking resistance in the united states today. the surveillance network. | from january to may 2000, as part of the tracking resistance in the united states today (trust) surveillance initiative, clinical isolates of enterobacteriaceae (n=2519) and non-fermentative gram-negatives (n=580) were prospectively collected from 26 hospital laboratories across the united states. isolates were tested for susceptibility to three fluoroquinolones (ciprofloxacin, levofloxacin, gatifloxacin) and seven other agents. in addition, data for the same period were collected from the surve ... | 2002 | 11814764 |
| [update on acinetobacter baumannii]. | resistance to imipenem: the increase in the prevalence of a. baumannii resistance is worrying because of the diversity of the enzymes implicated in the resistance and its epidemic diffusion that is always difficult to handle. broad spectrum beta-lactamase: still rare in acinetobacter, a broad spectrum beta-lactamase has been demonstrated in a strain isolated during a urinary infection and at the origin of an outbreak. risk factors: other than the classic risk factors, a survey regrouping the dat ... | 2002 | 12497720 |
| identification of acinetobacter species isolated from clinical specimens by amplified ribosomal dna restriction analysis. | taxonomy of acinetobacter has been changing ever since it was recognized to be associated with human infections. many biochemical schemes and molecular methods have been used for the species identification of this bacterium. recently a simple molecular method called amplified ribosomal dna restriction analysis (ardra) has been used to determine the genomospecies of acinetobacter: an attempt is made in the present study to identify the acinetobacter genomospecies isolated from clinical specimens ... | 2002 | 12514971 |
| infections caused by acinetobacter species and their susceptibility to 14 antibiotics in lagos university teaching hospital, lagos. | acinetobacter spp are well recognised as causes of nosocomial infections particularly in patients with immature or defective body defence system. information concerning these organisms are lacking in this environment. for this reason the pattern of infection and the antimicrobial susceptibility profiles of these organisms isolated over a one-year period were studied. a total of 58 (3%) of the 2001 isolates from all clinical specimens received in the laboratory during the year were acinetobacter ... | 2002 | 12744574 |
| plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different verticillium host plants. | to study the effect of plant species on the abundance and diversity of bacterial antagonists, the abundance, the phenotypic diversity, and the genotypic diversity of rhizobacteria isolated from potato, oilseed rape, and strawberry and from bulk soil which showed antagonistic activity towards the soilborne pathogen verticillium dahliae kleb. were analyzed. rhizosphere and soil samples were taken five times over two growing seasons in 1998 and 1999 from a randomized field trial. bacterial isolates ... | 2002 | 12089011 |
| characterization and movement of the class 1 integron known as tn2521 and tn1405. | two putative transposons, tn2521 and tn1405, carrying determinants for the pse-4 beta-lactamase and for resistance to streptomycin, spectinomycin, and sulfonamides were previously isolated from the chromosome of pseudomonas aeruginosa dalgleish. detailed mapping and determination of the complete sequence of tn2521 revealed that it is a class 1 integron, here renamed in33, with a backbone structure identical to that of in4 from tn1696. in33 contains two gene cassettes, blap1 and aada1, replacing ... | 2002 | 11959558 |
| [etiology of hospital-acquired infections in spanish hospitals (epine, 1990-1999)]. | clinical and demographic characteristics of patients, their interaction with pathogens and antimicrobial therapies are prompting changes in the epidemiology of hospital-acquired infections (hi). the knowledge of the etiology of hospital-acquired infections is valuable for the treatment of infected patients and for the prevention of hi.patients and method: we analyzed a series of 10 annual prevalence studies during the period 1990-1999 (epine project) in spanish hospitals. estimate of prevalence ... | 2002 | 12049704 |
| characterization of an integron carrying a new class d beta-lactamase (oxa-37) in acinetobacter baumannii. | integrons from a clinical strain of acinetobacter baumannii highly resistant to beta-lactams have been analyzed. the largest (2.2 kb) contained three gene cassettes: an aaca4, an open reading frame of 417 nucleotides, and an oxa-type encoding gene. the oxa gene nucleotide sequence differed from that of the oxa-20 in 2 bp, one of the mutations being silent. the nonsilent mutationgenerated a substitution of glutamic acid for aspartic acid. the new oxa has been named oxa-37. | 2002 | 12523622 |
| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| origin and evolution of the ampc beta-lactamases of citrobacter freundii. | to determine whether the widespread clinical use of beta-lactams has been selective for citrobacter freundii-derived alleles of plasmid ampc genes, we generated a bayesian consensus phylogeny of the published ampc sequences and compared the mics of 16 beta-lactam antibiotics for escherichia coli strains containing cloned copies of the c. freundii ampc alleles. we found that for the majority of compounds investigated, there has been essentially no increase in beta-lactam resistance conferred by t ... | 2002 | 11959544 |
| occurrence of antibiotic resistance gene cassettes aac(6')-ib, dfra5, dfra12, and erea2 in class i integrons in non-o1, non-o139 vibrio cholerae strains in india. | molecular mechanisms of multidrug resistance in vibrio cholerae belonging to non-o1, non-o139 serogroups isolated during 1997 to 1998 in calcutta, india, were investigated. out of the 94 strains examined, 22 strains were found to have class i integrons. the gene cassettes identified were dfra1, dfra15, dfra5, and dfra12 for trimethoprim; aac(6')-ib for amikacin and tobramycin; aada1 and aada2 for streptomycin and spectinomycin; and erea2 for erythromycin resistance. to our knowledge, this is the ... | 2002 | 12183252 |
| epidemiological typing and prevalence of integrons in multiresistant acinetobacter strains. | seventy-seven acinetobacter isolates were recovered from patients in a korean hospital during the period from november to december 1998. the isolates were genotyped using randomly amplified polymorphic dna (rapd) analysis for epidemiological relationship, and investigated for antibiotic susceptibility and presence of integrons. sixty-nine acinetobacter baumannii isolates were distributed into five groups by rapd profiles, with 5, 1, 60, 2 and 1 in each group. the major rapd group of 60 isolates ... | 2002 | 12076278 |
| [interpretative reading of the non-fermenting gram-negative bacilli antibiogram]. | among non-fermenting gram-negative rods, the most clinically important species are pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia, which are frequently multiresistant. p. aeruginosa resistance to beta-lactams depends on the production of chromosomal and plasmid-mediated beta-lactamases, altered permeability (loss of oprd porin is related to carbapenem-resistance) and active efflux pumps, particularly mexab-oprm. in aminoglycoside-resistant strains the main mech ... | 2002 | 12084355 |
| efficient plastid transformation in tobacco using the apha-6 gene and kanamycin selection. | here we report on the development of a new dominant selection marker for plastid transformation in higher plants using the aminoglycoside phosphotransferase gene apha-6 from acinetobacter baumannii. vectors containing chimeric apha-6 gene constructs were introduced into the tobacco chloroplast using particle bombardment of alginate-embedded protoplast-derived micro colonies or polyethylene glycol (peg)-mediated dna uptake. targeted insertion into the plastome was achieved via homologous recombin ... | 2002 | 12242495 |
| outbreak of a susceptible strain of acinetobacter species 13 (sensu tjernberg and ursing) in an adult neurosurgical intensive care unit. | between december 1999 and june 2000, an outbreak caused by acinetobacter emerged on the neurosurgical intensive care unit of our hospital. it was shown using automated ribotyping using eco ri and pulsed-field gel electrophoresis that the outbreak was caused by spread of a single strain, which was identified by ribotyping and amplified ribosomal dna restriction analysis as acinetobacter dna group 13tu (sensu tjernberg and ursing). the outbreak strain, which showed no antibiotic resistance, was id ... | 2002 | 12090795 |
| antimicrobial drug use and resistance in dogs. | fifteen years (1984-1998) of records from a veterinary teaching hospital were analyzed to determine whether antimicrobial drug resistance in coagulase-positive staphylococcus spp. (s. aureus, s. intermedius) isolated from clinical infections in dogs has increased, and whether there has been a change in the species of bacteria isolated from urinary tract infections in dogs. in coagulase-positive staphylococcus spp., a complex pattern showing both increases and decreases of resistance to different ... | 2002 | 11842592 |
| blactx-m-2 is located in an unusual class 1 integron (in35) which includes orf513. | examination of the bla(ctx-m-2) gene in plasmid pmar-12 by sequencing and pcr analysis revealed that the bla gene and the surrounding dna, which is closely related (99% homology) to the kluyvera ascorbata chromosomal dna that contains the bla(klua-1) gene, are located in a complex sul1-type integron, termed in35, that includes orf513. it is possible that bla(ctx-m-2) was acquired by plasmid pmar-12 through an uncharacterized recombinational event in which orf513 could be involved. | 2002 | 12069995 |
| tiamulin activity against fastidious and nonfastidious veterinary and human bacterial isolates: initial development of in vitro susceptibility test methods. | tiamulin is a pleuromutilin derivative used in veterinary practice for the control and specific therapy of infections in swine. this report summarizes studies to establish standardized susceptibility testing methods, interpretive criteria, and reagent details for use in veterinary methods recently developed by the national committee for clinical laboratory standards (nccls) (standards m31-a and m37-a, nccls, wayne, pa., 1999). a total of 636 fastidious and nonfastidious animal and human pathogen ... | 2002 | 11825957 |
| in vitro activities of tigecycline (gar-936) against recently isolated clinical bacteria in spain. | the antimicrobial activities of tigecycline (gar-936) were compared with those of other agents against 1,087 strains recently isolated in 12 spanish medical centers. tigecycline showed activity against a wide spectrum of aerobic and anaerobic bacteria, including strains such as methicillin-resistant staphylococcus aureus, coagulase-negative staphylococci, penicillin-resistant streptococcus pneumoniae, enterococcus faecium, acinetobacter baumannii, and stenotrophomonas maltophilia. | 2002 | 11850282 |
| the structure of the carbohydrate backbone of the lipopolysaccharide from acinetobacter baumannii strain atcc 19606. | the chemical structure of the phosphorylated carbohydrate backbone of the lipopolysaccharide (lps) from acinetobacter baumannii strain atcc 19606 was investigated by chemical analysis and nmr spectroscopy of oligosaccharides obtained after deacylation or mild acid hydrolysis. from the combined information the following carbohydrate backbones can be deduced: where r1 = h and r2 = alpha-glcp-(1-->2)-beta-glcp-(1-->4)-beta-glcp-(1-->4)-beta-glcp-(1 as major and r1 = ac and r2 = h as minor products. ... | 2002 | 11856300 |
| emerging carbapenemases in gram-negative aerobes. | carbapenemases may be defined as beta-lactamases that significantly hydrolyze at least imipenem or/and meropenem. carbapenemases involved in acquired resistance are of ambler molecular classes a, b, and d. class a, clavulanic acid-inhibited carbapenemases are rare. they are either chromosomally encoded (nmc-a, sme-1 to sme-3, imi-1) in enterobacter cloacae and serratia marcescens, or plasmid encoded, such as kpc-1 in klebsiella pneumoniae and ges-2 in pseudomonas aeruginosa, the latter being a p ... | 2002 | 12084099 |
| hospital-acquired infections following the 1999 marmara earthquake. | in this study, medical records of all casualties admitted to our hospital following the marmara earthquake, which struck northwest turkey and resulted in the destruction of several towns in the marmara region, were evaluated retrospectively. the time buried under the rubble, demographic data, type of medical and surgical therapies performed, type of injury and data on infection were analysed. between 17 august and 25 september 1999, 630 trauma victims were received at our hospital and 532 (84%) ... | 2002 | 12009820 |
| 3: community-acquired pneumonia. | community-acquired pneumonia is caused by a range of organisms, most commonly streptococcus pneumoniae, mycoplasma pneumoniae, chlamydia pneumoniae and respiratory viruses. chest x-ray is required for diagnosis. a risk score based on patient age, coexisting illness, physical signs and results of investigations can aid management decisions. patients at low risk can usually be managed with oral antibiotics at home, while those at higher risk should be further assessed, and may need admission to ho ... | 2002 | 12013330 |
| identification of acinetobacter isolates from species belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex with monoclonal antibodies specific for o antigens of their lipopolysaccharides. | the unambiguous identification of acinetobacter strains, particularly those belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex, is often hindered by their close geno- and phenotypic relationships. in this study, monoclonal antibodies (mabs) against the o antigens of the lipopolysaccharides from strains belonging to the a. calcoaceticus-a. baumannii complex were generated after the immunization of mice with heat-killed bacteria and shown by enzyme immunoassays and wester ... | 2002 | 11777830 |
| acinetobacter baumannii pseudomeningitis. | 2002 | 11805753 | |
| chromosome-encoded class d beta-lactamase oxa-23 in proteus mirabilis. | ten nonrepetitive proteus mirabilis isolates, which were collected over 4 years (1996 to 1999) at the teaching hospital of clermont-ferrand, france, produced class d carbapenemase oxa-23. mics of imipenem were 0.25 to 0.5 microg/ml for these clinical isolates. molecular typing revealed that the 10 p. mirabilis isolates originated from the same clonal strain. hybridization of i-ceui-generated chromosome fragments with a bla(oxa-23) probe showed that the gene was chromosome encoded in the p. mirab ... | 2002 | 12019126 |
| gastritis and hypergastrinemia due to acinetobacter lwoffii in mice. | in mouse models and humans, helicobacter pylori is associated with an increase in serum gastrin and gastrin-expressing (g) cells with a concomitant decrease in somatostatin-expressing d cells. inflammation of the gastric mucosa can progress to metaplastic changes in the stomach and to decreased colonization by h. pylori and increased colonization by non-h. pylori organisms. in addition, about 20% of individuals with chronic gastritis are h. pylori negative, suggesting that other organisms may in ... | 2002 | 11953405 |
| effects of t4 lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors. | rhizosphere bacterial communities of two transgenic potato lines which produce t4 lysozyme for protection against bacterial infections were analyzed in comparison to communities of wild-type plants and transgenic controls not harboring the lysozyme gene. rhizosphere samples were taken from young, flowering, and senescent plants at two field sites in three consecutive years. the communities were characterized in a polyphasic approach. cultivation-dependent methods included heterotrophic plate cou ... | 2002 | 11872484 |
| novel pcr-probe assay for detection of and discrimination between legionella pneumophila and other legionella species in clinical samples. | 2002 | 11880461 |