Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [presence of beta-lactamase with a broad spectrum in acinetobacter baumanii]. | 1990 | 2139952 | |
| species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing acinetobacter strains. | species, biotypes, and phage types were determined for 120 acinetobacter strains from clinical or environmental sources or from culture collections. these characteristics were compared with cell envelope protein profiles obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in previous studies. a considerable heterogeneity of species and types was observed by use of the various methods, in particular among strains from different sources. acinetobacter baumannii was the most commo ... | 1990 | 2179253 |
| serotyping of clinical isolates of acinetobacter: serovars of genospecies 3. | a total of 156 clinical isolates of genospecies 3, the second most commonly encountered member of the genus acinetobacter at our institution, were identified with carbon source utilization tests among 400 acinetobacter isolates. checkerboard tube agglutination tests and reciprocal cross-absorption studies with polyclonal rabbit immune sera against heated (100 degrees c, 1 h) cells of serovar candidate strains of genospecies 3 permitted identification of 13 serovars. minor one-way crossreactions ... | 1990 | 2363794 |
| cell surface hydrophobicity of 88 clinical strains of acinetobacter baumannii. | we studied the surface hydrophobicity of 88 acinetobacter baumannii strains of clinical origin, using both salt aggregation and adherence to paraxylene tests. strains were divided into 2 groups: the first included 65 strains isolated from various clinical samples (infected catheters, tracheal and bladder devices); the second included 23 strains isolated from skin obtained from healthy controls. high surface hydrophobicity was observed in 92% of the first group of strains and in only 5% of the se ... | 1990 | 2399369 |
| epidemic bacteremia due to acinetobacter baumannii in five intensive care units. | from march 5, 1986 to september 4, 1987, acinetobacter baumannii (ab) was isolated from blood or vascular catheter-tip cultures of 75 patients in five intensive care units at a hospital in new jersey. to identify risk factors for ab bacteremia in the intensive care units, a case-control study was conducted. characteristics of 72 case-patients were compared with those of 37 controls. case-patients were more likely than controls to have had peripheral arterial catheters (odds ratio (or) = 7.0, p l ... | 1990 | 2403113 |
| identification of an acinetobacter baumannii gene region with sequence and organizational similarity to tn2670. | a chloramphenicol resistance gene was cloned from chromosomal dna prepared from a clinical acinetobacter baumannii isolate. sequence analysis of this gene (cat) and the flanking dna regions shows that this gene is linked to tn21 and to is1 in a manner similar to that found in tn2670. | 1991 | 1650008 |
| serotyping of clinical isolates of acinetobacter baumannii and genospecies 3: detection of additional serovars. | continued serological monitoring of clinical isolates of acinetobacter baumannii and genospecies 3 permitted identification of 7 new serovars of a. baumannii and 8 additional serovars of genospecies 3. | 1991 | 1755922 |
| imipenem resistance in acinetobacter baumanii is due to altered penicillin-binding proteins. | the comparison of a clinical acinetobacter baumanii isolate (strain no. 4852/88) and its selected imipenem-resistant (imr) clone exhibited a complex reorganization of the penicillin-binding proteins (pbps) with diminished labelling of all pbps except the 24-kd pbp which showed an increased binding of 14c-penicillin. this protein could not be saturated by preincubation of membranes with imipenem at 8-fold the mic of imipenem, thus indicating pbp alterations responsible for imipenem resistance. in ... | 1991 | 1760939 |
| [presence of various acinetobacter species in urinary tract infections]. | species classification of 39 strains of acinetobacter isolated from urinary tract infections (uti) was performed by api 20ne tests. on the whole 24 biotypes were differentiated which formed 4 species and one biotype had no defined species classification. acinetobacter baumani was most numerously represented. seventy seven percent of strains were isolated from urine samples from women. about 90% of acinetobacter strains were isolated as single pathogens. sensitivity of these strains to 23 antibio ... | 1991 | 1823373 |
| [nosocomial infections in an urological department. incidence and etiological factors]. | the authors report a prospective study with active request of data for nosocomial infections (ni) in an urological department during six months. from 453 patients, 43 developed an ni (incidence = 9.5%): urinary tract infections (53.5%), bloodstream infections (16.3%), lower respiratory tract infections (7%), surgical wound infections (2.3%) and sepsis syndrome (20.9%). for microorganisms, most often pseudomonas aeruginosa (22.5%) and e. coli (20%) were encountered. other microorganisms were the ... | 1991 | 1909021 |
| comparative antimicrobial spectrum and activity of ceftibuten against clinical isolates from west germany. | the in vitro activity of a new oral cephalosporin, ceftibuten, was determined against 837 clinical isolates by agar dilution technique and compared with that of the oral cephalosporins, cefaclor, cefuroxime, cefixime, cefpodoxime, and cefprozil. against enterobacteriaceae, ceftibuten was the most active of the compounds. ceftibuten mic90s were less than or equal to 0.25 micrograms/ml for most members of the family enterobacteriaceae, 0.13 microgram/ml for haemophilus influenzae, 4 micrograms/ml ... | 1991 | 2013211 |
| [occurrence of species from acinetobacter genus in clinical material and other sources]. | the analysis regarded 304 strains of acinetobacter genus isolated from various diagnostic materials, objects from hospital environment and from non-hospital sources (soil, water, various animals). applying api zone system, five species were isolated: acinetobacter juni, (18.42%), acinetobacter baumanii (70.39%), acinetobacter haemolyticus (5.59%), acinetobacter lwoffii (4.6%) and acinetobacter johnsonii (0.99%). most frequently isolated species were present in purulent materials and in samples f ... | 1992 | 1297033 |
| bactericidal activity of ciprofloxacin upon escherichia coli and acinetobacter baumanni. | the mechanisms of bactericidal activity of ciprofloxacin (mechanisms a and b) upon cells of a strain of escherichia coli and one strain of acinetobacter baumannii were investigated under different conditions. the killing of e. coli cells by ciprofloxacin was significantly reduced by chloramphenicol, but this antibiotic showed almost no activity upon killing of a. baumannii cells by this quinolone. similar results were obtained when rifampicin was added to ciprofloxacin. bactericidal activity of ... | 1992 | 1345113 |
| use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of acinetobacter baumannii. | genomic dnas from taxonomically and epidemiologically well-defined strains of acinetobacter baumannii were digested with restriction endonucleases that cleave with low frequency, and the fragments were separated by pulse-field gel electrophoresis. restriction fragment length polymorphisms were observed. restriction fragment length polymorphism analysis can be used as an epidemiological tool to delineate outbreaks of nosocomial infections caused by a. baumannii. | 1992 | 1352519 |
| ribotyping of the acinetobacter calcoaceticus-acinetobacter baumannii complex. | the acinetobacter calcoaceticus-acinetobacter baumannii complex consists of four genotypically distinct but phenotypically very similar bacterial species or dna groups: a. calcoaceticus (dna group 1), a. baumannii (dna group 2), unnamed dna group 3 (p. j. m. bouvet and p. a. d. grimont, int. j. syst. bacteriol. 36:228-240, 1986), and unnamed dna group 13 (i. tjernberg and j. ursing, apmis 97:595-605, 1989). because strains in this complex cause nosocomial outbreaks, it is important to be able to ... | 1992 | 1383266 |
| esterase electrophoresis compared with biotyping for epidemiological typing of acinetobacter baumannii strains. | forty-nine acinetobacter baumannii strains belonging to three biotypes and isolated from four hospitals were differentiated by electrophoretic typing of their esterases. six main kinds of esterases were distinguished by their spectra of hydrolytic activity toward seven synthetic substrates. the electrophoretic variations of these enzymes were used to define ten zymotypes among the three biotypes. esterase electrophoresis appeared to be more sensitive than biotyping, and could represent an additi ... | 1992 | 1398027 |
| treatment of experimental infections of mice with bacteriophages. | bacteriophages for acinetobacter baumanii, pseudomonas aeruginosa and staphylococcus aureus were tested in experimental infections of mice to investigate their potential for the treatment of infections of man. as few as 10(2) particles of an acinetobacter phage protected mice against 5 ld50 (1 x 10(8)) of a virulent strain of a. baumanii, and phage was demonstrated to have multiplied in the mice. a pseudomonas phage protected mice against 5 ld50 of a virulent strain of p. aeruginosa, with a pd50 ... | 1992 | 1404324 |
| characterization of a high-affinity iron transport system in acinetobacter baumannii. | analysis of a clinical isolate of acinetobacter baumannii showed that this bacterium was able to grow under iron-limiting conditions, using chemically defined growth media containing different iron chelators such as human transferrin, ethylenediaminedi-(o-hydroxyphenyl)acetic acid, nitrilotriacetic acid, and 2,2'-bipyridyl. this iron uptake-proficient phenotype was due to the synthesis and secretion of a catechol-type siderophore compound. utilization bioassays using the salmonella typhimurium i ... | 1992 | 1447137 |
| in vitro interactions of aminoglycosides with imipenem or ciprofloxacin against aminoglycoside resistant acinetobacter baumannii. | the in vitro interactions between gentamicin, tobramycin, netilmicin and amikacin with imipenem and ciprofloxacin were evaluated by the killing curve technique against 20 clinical isolates of acinetobacter baumannii highly resistant to aminoglycosides which were susceptible or moderately susceptible to imipenem and resistant or moderately susceptible to ciprofloxacin. imipenem enhanced killing by gentamicin, tobramycin, netilmicin and amikacin in tests with 9, 12, 10 and 15 strains (45-75%) whil ... | 1992 | 1479414 |
| the clinical significance of acinetobacter baumannii in blood cultures. | acinetobacter baumannii was isolated from blood cultures in 31 patients in 4 intensive care units at a large teaching hospital over a period of 6 months. in 23 of these patients, acinetobacter was also isolated from tracheal aspirates. catheter-related infection could be identified as the cause of bacteremia in 14 cases, whereas pneumonia was present in only 6 cases. all patients were seriously ill and 7 (24%) died of their infection. | 1992 | 1520980 |
| community-acquired acinetobacter pneumonia in the northern territory of australia. | eleven cases of blood culture-positive, community-acquired pneumonia due to the human commensal acinetobacter baumannii were studied in darwin in the northern territory of australia during the 10-year period from march 1981 through february 1991. demographic risk factors included male gender, age of greater than 45 years, and aboriginal ethnic background. multiple clinical risk factors, including cigarette smoking, alcoholism, chronic obstructive airway disease, and diabetes mellitus, were noted ... | 1992 | 1571467 |
| identification of an epidemic strain of acinetobacter baumannii using electrophoretic typing methods. | nosocomial infections due to acinetobacter baumannii dramatically increased in a lebanese medical center following an outbreak of hostilities in lebanon in 1984. the incidence of infection caused by this organism has remained high in this institution, thus requiring the implementation of a strain typing system to aid in infection control. three methods were investigated for their utility in differentiating among a representative group of 36 nosocomial acinetobacter baumannii isolates obtained ov ... | 1992 | 1572437 |
| ru 29 246, the active compound of the cephalosporin-prodrug-ester hr 916. i. antibacterial activity in vitro. | the aminothiazolyl-cephalosporin ru 29 246 is the active metabolite of the prodrug-pivaloyl-oxyethyl-ester hr 916. ru 29 246 in vitro activity includes a wide range of clinically relevant bacterial pathogens. against methicillin-sensitive staphylococci ru 29 246 (mic90 of 0.25 approximately 2 micrograms/ml) was clearly more active than cefaclor, cefuroxime, cefpodoxime, cefixime and ceftibuten, but slightly less active than cefdinir. ru 29 246 inhibited hemolytic streptococci of the serogroups a ... | 1992 | 1592683 |
| delayed detection of an increase in resistant acinetobacter at a detroit hospital. | to study an increase of antimicrobial-resistant acinetobacter baumannii and to assess reasons for the delayed detection of this increase. | 1992 | 1640096 |
| comparative in-vitro activities of the new quinolone, bay y 3118, and ciprofloxacin, sparfloxacin, tosufloxacin, ci-960 and ci-990. | the in-vitro activity of the new quinolone, bay y 3118, was compared with that of ciprofloxacin, tosufloxacin, sparfloxacin, ci-960 and ci-990 against 1640 isolates belonging to 117 bacterial species. against members of the enterobacteriaceae, bay y 3118 was as active as ci-960 and ci-990, up to four-fold more active than ciprofloxacin and tosufloxacin and up to 16-fold more active than sparfloxacin. the majority of enterobacteriaceae which were resistant to ciprofloxacin (mics > or = 2 mg/l) we ... | 1993 | 7605398 |
| lipopolysaccharide profile typing as a technique for comparative typing of gram-negative bacteria. | we have applied the technique of lipopolysaccharide (lps) profiling in sodium dodecyl sulfate-polyacrylamide gel electrophoresis to the typing of 124 isolates of 12 gram-negative species from suspected outbreaks of infection. lps was prepared by proteinase k digestion or micro-phenol-water extraction. a total of 11 of the 12 species gave clear ladder band profiles, the exception being acinetobacter baumannii. when compared with conventional typing for enterobacter cloacae, pseudomonas aeruginosa ... | 1993 | 7684751 |
| opportunistic nosocomial multiply resistant bacterial infections--their treatment and prevention. | one of the most difficult problems confronting the clinician who deals with nosocomial infections is that of microbial resistance. the predominant nosocomial infections (urinary tract infections, pneumonia, septicaemia, surgical wound infections) involve increasing numbers of gram-positive bacteria, staphylococcus epidermidis, corynebacterium jeikeium or resistant enterococci as well as new multiresistant gram-negative bacilli such as xanthomonas maltophilia, acinetobacter baumannii and alcalige ... | 1993 | 7755661 |
| immunobiology of acinetobacter baumannii and genospecies 3. | five representative, taxonomically and serologically defined clinical isolates of acinetobacter baumannii and genospecies 3, and a. baumannii strain atcc 19606 were examined for immunogenicity in rabbits following experimental bacteremia. all rabbits seroconverted as determined with the aid of the tube o-agglutination, indirect hemagglutination, and enzyme-linked immunosorbent assay (elisa) procedures. immunoblots detected over twenty immunogenic, proteinase-k-degradable polypeptide antigens in ... | 1993 | 8219496 |
| the digestive tract is a major site for acinetobacter baumannii colonization in intensive care unit patients. | 1993 | 8228382 | |
| effect of iron-limiting conditions on growth of clinical isolates of acinetobacter baumannii. | different clinical isolates of acinetobacter baumannii, typed by plasmid profile, were able to grow in iron-chelated medium by secreting iron-regulated siderophores. this iron-scavenging phenotype was associated with the production of iron-repressible catechol. siderophore utilization bioassays showed the presence of 2,3-dihydroxybenzoic acid in the growth medium, and neither enterobactin nor aerobactin was detected in culture supernatants obtained under iron-deficient conditions. | 1993 | 8253994 |
| erythromycin, clarithromycin, and azithromycin: use of frequency distribution curves, scattergrams, and regression analyses to compare in vitro activities and describe cross-resistance. | mics of erythromycin, clarithromycin, and azithromycin for 852 recent clinical isolates were determined by broth microdilution methods. frequency distribution curves, scattergrams, and regression analyses were used to compare in vitro activities and describe cross-resistance. clarithromycin was the most active drug against bacteroides spp. but the least active against haemophilus influenzae. azithromycin was most active against h. influenzae, moraxella catarrhalis, pasteurella multocida, and fus ... | 1993 | 8257127 |
| characterization of acinetobacter haemolyticus aac(6')-ig gene encoding an aminoglycoside 6'-n-acetyltransferase which modifies amikacin. | the amikacin resistance gene acc(6')-ig of acinetobacter haemolyticus bm2685 encoding an aminoglycoside 6'-n-acetyltransferase was characterized. the gene was identified as a coding sequence of 438 bp corresponding to a protein with a calculated mass of 16,522 da. analysis of the deduced amino acid sequence suggested that it was the fourth member of a subfamily of aminoglycoside 6'-n-acetyltransferases. the resistance gene was not transferable either by conjugation to escherichia coli or to acin ... | 1993 | 8257129 |
| [epidemiology of infections caused by acinetobacter baumannii]. | 1993 | 8260518 | |
| vascular catheter-related bloodstream infection due to acinetobacter johnsonii (formerly acinetobacter calcoaceticus var. lwoffi): report of 13 cases. | although acinetobacter baumannii (formerly acinetobacter calcoaceticus var. anitratus) is known to be an important nosocomial pathogen, the clinical impact of other acinetobacter species is not fully understood. acinetobacter johnsonii (formerly a subset of a. calcoaceticus var. lwoffii) is considered a commensal on human skin, and infections due to this organism have not been reported previously. during a study period of 18 months, however, 13 patients at 6 hospitals developed catheter-related ... | 1993 | 8268343 |
| genetic diversity and clonal relationships of acinetobacter baumannii strains isolated in a neonatal ward: epidemiological investigations by allozyme, whole-cell protein and antibiotic resistance analysis. | sixty-five strains of acinetobacter baumannii which had been isolated from patients and the indoor environment of a neonatal intensive care unit and, for comparative purposes, isolates from three other wards, were examined by means of electrotyping and analysis of whole-cell protein and antibiotic resistance patterns. fourteen different electrotypes were determined. the predominant type, a multiply resistant acinetobacter clone, persisted in the neonatal ward over several months. the results und ... | 1993 | 8270009 |
| the structure of the chloramphenicol resistance gene on a transferable r plasmid from the fish pathogen, pasteurella piscicida. | the chloramphenicol resistance gene (pp-cat) was cloned from a transferable r plasmid of pasteurella piscicida, psp9351, and the sequence of the gene was determined. subcloning and deletion analysis localized the resistance gene, pp-cat, to within a 2.3 kb hincii-bamhi fragment. the fragment as a probe hybridized with the type i chloramphenicol acetyltransferase (cat) gene and did not hybridize to cat types ii, iii, and cat-va. the fragment hybridized to transferable r plasmids encoded with resi ... | 1993 | 8271973 |
| in vitro activity of bay y 3118, a new quinolone. | mics of bay y 3118, ciprofloxacin, ofloxacin, clarithromycin, azithromycin, cefuroxime, amoxicillin-clavulanate, and trimethoprim-sulfamethoxazole for 878 recent clinical isolates were determined by broth microdilution methods. among the three quinolones, bay y 3118 was the most active against haemophilus influenzae, moraxella catarrhalis, acinetobacter baumannii, xanthomonas maltophilia, gram-positive cocci, and anaerobes; mics for 50% of the strains (mic50s) and mic90s were < or = 0.015 and < ... | 1993 | 8285618 |
| acinetobacter in denmark: ii. molecular studies of the acinetobacter calcoaceticus-acinetobacter baumannii complex. | the acinetobacter calcoaceticus-acinetobacter baumannii complex consists of four closely related "genospecies" or dna groups: dna group 1 (a. calcoaceticus), dna group 2 (a. baumannii), dna 3, and tjernberg & ursing's dna group 13. strains in this complex are so similar phenotypically that it is often impossible to identify them to the dna group level by the use of biochemical tests. twenty-three danish clinical strains from 23 patients phenotypically identified to the a. calcoaceticus-a. bauman ... | 1993 | 8286091 |
| nosocomial acinetobacter meningitis secondary to invasive procedures: report of 25 cases and review. | the medical records of 25 patients with nosocomial meningitis due to acinetobacter baumannii were retrospectively reviewed. most cases occurred in the neurosurgical intensive care unit over a 5-year period, with an increased rate during summer. the majority of infections were associated with indwelling ventriculostomy tubes or csf fistulae in patients receiving antimicrobial therapy. repeated environmental cultures failed to reveal a source of the microorganism, and control measures had no appar ... | 1993 | 8286623 |
| [antimicrobial activity of amoxicillin versus amoxicillin/clavulanic acids. in vitro study against s aureus, h influenzae and a baumanii strains]. | the aim of this study was to compare in vitro activity, measuring minimal inhibitory concentration, of amoxicillin or its combination with clavulanic acid against 109 strains of acinetobacter baumanii, 104 strains of hemophilus influenzae and 94 strains of staphylococcus aureus. all these were obtained from different hospitals of the santiago metropolitan region. amoxicillin-clavulanic acid association did not improve the activity of amoxicillin against acinetobacter. the association was not act ... | 1993 | 8296100 |
| [ciprofloxacin resistance in gram negative bacilli. epidemiologic aspects]. | the aim of the present was to study the resistance to ciprofloxacin (cip) in gram negative bacilli (april 1990-march 1992) and to determine the temporary distribution of the resistant strains by species, samples and departments. | 1993 | 8305553 |
| [community-acquired acinetobacter pneumonia]. | we report the history of a 38-year-old male native of sri lanka admitted to the emergency ward because of chest pain and shortness of breath. on physical and radiographic examination a bilateral predominantly right-sided pneumonia was found. the patient was admitted to the medical icu and an antibiotic regimen with amoxicillin/clavulanic acid and erythromycin was initiated. shortly afterwards septic shock developed. the patient was intubated and received high doses of catecholamines. he died 30 ... | 1993 | 8372343 |
| epidemiological study of an acinetobacter baumannii outbreak by using polymerase chain reaction fingerprinting. | a polymerase chain reaction (pcr) technique was applied to the fingerprinting of different strains of acinetobacter baumannii from a cluster of patients infected or colonized with the incriminated pathogen. the dna was extracted by boiling and was subjected to pcr amplification by using the core sequence of the m13 phase as a single primer. the amplified products were separated by agarose gel electrophoresis and were detected by staining with ethidium bromide. in 1990, 49 multiresistant a. bauma ... | 1993 | 8408565 |
| in vitro antimicrobial production of beta-lactamases, aminoglycoside-modifying enzymes, and chloramphenicol acetyltransferase by and susceptibility of clinical isolates of acinetobacter baumannii. | antimicrobial susceptibility testing was performed on 54 epidemiologically unrelated clinical isolates of acinetobacter baumannii by using a standard agar dilution technique. on the basis of the in vitro activities, imipenem and doxycycline were the most active agents, whereas amikacin, isepamicin, and the new fluorquinolones ciprofloxacin and ofloxacin presented moderate activity. cephalosporinase activity was found in 98% of the strains, whereas lactamases of tem type 1 and one with a pi of 7 ... | 1993 | 8431011 |
| [a urinary outbreak of acinetobacter baumanii in a spinal cord injury unit]. | from january 1990 to april 1992, 114 urinary strains of acinetobacter baumanii were isolated in 57 patients with traumatic spinal cord [correction of medular] injury. the strains were characterized by having all of them the same biochemical identification, except for citrate, maltose and tryptophan-desaminase. until december 1990, (5 strains) were resistant to all antibiotics, except to tobramicine, amikacine, cotrimoxazol and imipenem (6.3%, 33.9%, 26.7% and 0% of resistances, respectively); si ... | 1993 | 8452972 |
| [epidemic occurrences of multiresistant acinetobacter baumannii strains in a neonatal intensive care unit]. | in a period of nine months (may 1991 to january 1992), 39 infants were colonized with acinetobacter baumannii in a paediatric intensive care unit. colonization was observed mainly in premature infants, weighing between 680 g and 2,000 g, who were artificially ventilated. shortly after birth, a. baumannii was isolated regularly from tracheal washings, and less frequently from other material, such as gastric juice, catheter tips, and umbilical swabs. in older children or adults, the bacteria were ... | 1993 | 8476498 |
| antimicrobial susceptibility of acinetobacter species. | the in vitro activities of 16 antimicrobial agents against 180 acinetobacter strains isolated from blood cultures (n = 162), central venous catheters (n = 11), and cerebrospinal fluids (n = 7) were studied. mics were determined by a microtiter broth dilution method. considerable differences in antimicrobial drug susceptibility against strains belonging to different species could be demonstrated. acinetobacter baumannii isolates (n = 108) were generally more resistant than isolates identified as ... | 1993 | 8494371 |
| nosocomial colonization and infection with multiresistant acinetobacter baumannii: outbreak delineation using dna macrorestriction analysis and pcr-fingerprinting. | the prevalence of nosocomial acinetobacter colonization and infection in a university hospital was reviewed and multiresistant acinetobacter baumannii infections in an intensive care unit (icu) were investigated using epidemiological typing and a case-control study. acinetobacter colonization at various body sites was found in 3.2 to 10.8 per 1000 patients. acinetobacter infection accounted for 0.3% of endemic nosocomial infections in critically ill patients and for 1% of nosocomial bacteraemia ... | 1993 | 7901273 |
| in vitro antimicrobial activity of fluoroquinolones against clinical isolates obtained in 1989 and 1990. | the in vitro activity of nine fluoroquinolones, enoxacin, norfloxacin, ofloxacin, ciprofloxacin, lomefloxacin, tosufloxacin, sparfloxacin, fleroxacin and levofloxacin, and two earlier quinolones, nalidixic acid and pipemidic acid, against 1,346 bacterial strains isolated clinically between 1989 and 1990, was evaluated by agar dilution method. the bacteria studied were staphylococcus aureus (including methicillin-susceptible and -resistant strains), staphylococcus epidermidis, enterococcus specie ... | 1993 | 7911351 |
| comparison of three typing methods in hospital outbreaks of acinetobacter calcoaceticus infection. | during a period of 11 months acinetobacter baumanii was isolated from 27 patients and 21 environmental samples in the intensive care unit (icu) and in one surgical unit of a university hospital. the isolates were characterized by biotyping, antibiograms and plasmid profiles and compared with co-isolates. plasmid fingerprinting distinguished three outbreaks, whereas other typing methods were less sensitive and discriminatory. although plasmid profiles seem to be a simple and reproducible marker f ... | 1993 | 8097217 |
| gram-negative bacillary meningitis in adults: a recent six-year experience. | thirty-eight cases of gram-negative bacillary meningitis in adults have been identified over the past six years at the veterans general hospital, taipei. twenty cases were associated with head trauma and/or neurosurgery, while 18 cases occurred spontaneously. the overall mortality was 58%. within the spontaneous meningitis group, 13 cases (72%) were due to klebsiella pneumoniae, 11 cases (61%) were associated with bacteremia and eight cases (44%) with diabetes mellitus. in spite of the administr ... | 1993 | 8106042 |
| ari 1: beta-lactamase-mediated imipenem resistance in acinetobacter baumannii. | a strain of acinetobacter baumannii 6b92 isolated from the blood culture of a patient at the edinburgh royal infirmary i 1985 was found to be resistant to imipenem, all classes of cephalosporins and penicillins. extraction of the soluble proteins of the cell and isoelectric focusing revealed the presence of two beta-lactamases: a chromosomal cephalosporinase of high pi (> pi 9.0) and a novel beta-lactomase of pi 6.65 named ari 1 (acinetobacter resistant to imipenem). despite the fact that origin ... | 1993 | 18611526 |
| plasmid mediated silver resistance in acinetobacter baumannii. | acinetobacter baumannii bl88, an environmental isolate, was resistant to 13 metals and 10 antibiotics. plumbagin cured resistance to silver, cadmium, antimony, streptomycin and ampicillin at varying frequencies. however, only silver resistance transferred (1 x 10(-6) recepient-1) to escherichia coli k12 during conjugation. correspondingly there was transfer of a 54 kb plasmid (pupi199) from a. baumannii bl88. the plasmid transformed e. coli dh5 alpha cells at a frequency of 1 x 10(-8) recepient- ... | 1994 | 8118173 |
| plasmid dna fingerprinting of acinetobacter species other than acinetobacter baumannii. | during the last years acinetobacter species have emerged as clinically significant pathogens. most infections are nosocomially acquired and mainly due to acinetobacter baumannii. little is known about the epidemiology and clinical significance of unnamed acinetobacter species 3 (the second most often encountered member of the genus acinetobacter) and other acinetobacter species such as a. johnsonii, a. junii, and a. lwoffii. seventy-five clinical isolates of acinetobacter species other than a. b ... | 1994 | 8126208 |
| high level kanamycin resistance associated with the hyperproduction of aac(3)ii and a generalised reduction in the accumulation of aminoglycosides in acinetobacter spp. | the clinical isolate of acinetobacter baumannii strain sak contains an actively transcribed aacc2 gene and a latent aadb gene that encode the aminoglycoside-modifying enzymes, aac(3)ii and aad(2"), respectively. in an attempt to activate the aadb gene, the strain was cultured in the presence of kanamycin which is a substrate for aad(2"). although it was possible to isolate kanamycin resistant derivatives these were not associated with detectable aad(2") activity. instead, there was a marked incr ... | 1994 | 7868401 |
| comparison of four different methods for epidemiologic typing of acinetobacter baumannii. | a set of 103 epidemiologically well-defined acinetobacter baumannii isolates obtained from nine hospital outbreaks and 21 unrelated strains were characterized by pulsed-field gel electrophoresis (pfge) of total genomic dna digested with apai. among outbreak strains, eight different patterns and five possible variants were identified by pfge. results were compared with those from traditional typing methods such as plasmid profile analysis, antimicrobial susceptibility, and biotyping. plasmid anal ... | 1994 | 7929781 |
| in vitro susceptibility of acinetobacter baumannii to biapenem, piperacillin/tazobactam and thirteen other antimicrobial agents. | 1994 | 7957281 | |
| clinical and molecular epidemiology of acinetobacter infections sensitive only to polymyxin b and sulbactam. | a nosocomial outbreak of infections due to imipenem-resistant acinetobacter baumannii occurred in a new york hospital after increased use of imipenem for cephalosporin-resistant klebsiella infections. we identified all a baumannii isolates over 12 months, reviewed corresponding patient records, and compared strains with different antibiotic susceptibility patterns by restriction endonuclease analysis. environmental surveillance cultures were done before and after institution of control measures. ... | 1994 | 7968028 |
| plasmid dna profiles of acinetobacter baumannii: clinical application in a complex endemic setting. | to study the epidemiological, microbiological, and clinical features of infections due to acinetobacter baumannii in a complex endemic situation over an 18-month period and to determine the clinical usefulness of plasmid dna analysis of a baumannii in epidemiological investigations. | 1994 | 7983345 |
| [infection of burn wound with acinetobacter baumanii]. | this paper describes infection of burn wound with participation of acinetobacter baumanii in three out of five patients. species classification of 23 strains of acinetobacter was performed by application of api 20 ne tests. the profiles obtained through these tests permitted for selection of 8 biotypes. biotype 00010703 was isolated most frequently. all tested strains of acinetobacter sp. were susceptible only to netilmicin, norfloxacin, pefloxacin, ofloxacin, ciprofloxacin and imipenem. out of ... | 1994 | 7996933 |
| disseminated cutaneous botryomycosis--an unexpected diagnosis after 20-years' duration. | a 35-year-old man presented with nodular suppurative lesions of the buttocks and the neck evolving over 20 years. a diagnosis of botryomycosis was established. staphylococcus aureus, acinetobacter baumanii and coagulase negative staphylococcus were isolated from the biopsy specimen. surgical excision was performed with success on the buttock. | 1994 | 8033393 |
| structure of a surface polysaccharide from acinetobacter baumannii strain 214. | a polysaccharide containing d-galactose, d-glucose, and 2-acetamido-2-deoxy-d-galactose was obtained from an aqueous phenol extract of isolated cell walls from acinetobacter baumannii strain 214. by means of nmr studies and chemical degradations, the repeating unit of the polymer was identified as a branched trisaccharide of the structure shown. [formula: see text] | 1994 | 8039176 |
| biotyping, ribotyping and esterase electrophoresis as epidemiological tools for acinetobacter baumannii. | an epidemiological survey was carried out over a one-week period to assess the spread of acinetobacter baumannii in a medical intensive care unit. fifty strains were isolated from patients colonized or infected by the organism and from a hospital environment. these strains belonged to biotypes 9 or 18. the rrna gene restriction patterns (using ecori and pvuii as restriction endonucleases) and the esterase electrophoretic profiles were determined on 31 strains, using as comparison strain isolates ... | 1994 | 8090981 |
| isolation and structure elucidation of acinetobactin, a novel siderophore from acinetobacter baumannii. | a novel siderophore, called acinetobactin, with both catecholate and hydroxamate functional groups was isolated from low-iron cultures of acinetobacter baumannii atcc 19606. the structure was elucidated by chemical degradation, fast-atom bombardment mass spectrometry and 1h and 13c nmr spectroscopy. acinetobactin was composed of omega-n-hydroxyhistamine, threonine and 2,3-dihydroxybenzoic acid, the last two components forming an oxazoline ring. acinetobactin was structurally related to anguibact ... | 1994 | 7802543 |
| epidemiological markers of acinetobacter baumannii clinical isolates from a spinal cord injury unit. | during a period of 28 months, 114 isolates of acinetobacter baumannii obtained from urine samples of 57 patients, were recovered in a spinal cord unit; an unusual increase in the number of a. baumannii isolates was observed between february 1991 and january 1992. six different typing methods [biotyping, antimicrobial susceptibility, whole cell and cell-envelope protein analysis, plasmid analysis and chromosomal dna analysis by pulsed-field gel electrophoresis (pfge)] were used to study the isola ... | 1994 | 7806867 |
| characterization of the chromosomal aac(6')-ij gene of acinetobacter sp. 13 and the aac(6')-ih plasmid gene of acinetobacter baumannii. | the amikacin resistance genes aac(6')-ih of acinetobacter baumannii bm2686 and aac(6')-ij of acinetobacter sp. 13 bm2689 encoding aminoglycoside 6'-n-acetyltransferases were characterized. the 441-bp coding sequences predict proteins with calculated masses of 16,698 and 16,677 da, respectively. analysis of the deduced amino acid sequences indicated that the proteins belonged to a subfamily of 6'-aminoglycoside acetyltransferase type i enzymes from gram-negative bacteria. the aac(6')-ih gene of b ... | 1994 | 7810994 |
| cloning and expression in escherichia coli of the gene encoding a novel l-2,4-diaminobutyrate decarboxylase of acinetobacter baumannii. | the gene encoding l-2,4-diaminobutyrate decarboxylase (daba dc) was cloned from acinetobacter baumannii atcc 19606. the gene was evidently under the control of its own promoter. interestingly, the host carrying this clone also produced an appreciable amount of 1,3-diaminopropane. restriction mapping and subsequent subcloning of the cloned insert localized the daba dc gene within a 2.45-kb sphi/ecori fragment. for endogenous production of dap, a 1.75-kb ecori/psti region downstream from the daba ... | 1994 | 7813892 |
| antimicrobial susceptibilities of clinical isolates of acinetobacter baumannii from singapore. | the in vitro activities of 17 antimicrobial agents alone or in combination against 70 clinical isolates of acinetobacter baumannii from singapore were determined by broth microdilution. the mics of amoxicillin, ampicillin, ceftazidime, ceftriaxone, gentamicin, and piperacillin for 90% of the strains were > or = 128 micrograms/ml. addition of sulbactam to ampicillin produced improved activity, whereas adding tazobactam to piperacillin did not. the mics of amikacin, ciprofloxacin, and imipenem for ... | 1994 | 7840598 |
| identification of salmonella spp. with rambach agar in conjunction with the 4-methylumbelliferyl caprylate (mucap) fluorescence test. | the utility of rambach agar to identify salmonella spp. was examined relative to its usefulness in clinical microbiology. forty-four of 54 (82%) salmonella organisms isolated from faecal cultures and 66 of 82 (84%) salmonella stock cultures produced bright red colour colonies after 24 h incubation at 37 degrees c, whereas 48 of 54 (89%) salmonellae isolated from faecal cultures, and 74 of 82 (90%) salmonella stock cultures, yielded the bright red colour when the incubation time was extended to 4 ... | 1994 | 7841836 |
| discrimination of epidemic and sporadic isolates of acinetobacter baumannii by repetitive element pcr-mediated dna fingerprinting. | in 1990, there was a significant increase in the number of lower respiratory tract infections and surgical wound infections in the adult intensive care units of our tertiary care teaching hospital caused by acinetobacter baumannii compared with the number in 1989. during the 5-month period from april through august 1990, 84 isolates of a. baumannii were recovered from 50 hospitalized patients. biotyping, comparison of antibiograms, plasmid analysis, and dna polymorphisms of 20 isolates from 20 d ... | 1994 | 7852548 |
| characterization of a hospital outbreak of imipenem-resistant acinetobacter baumannii by phenotypic and genotypic typing methods. | during a 13-month period, 31 patients hospitalized primarily in two intensive care units (icus) were either colonized or infected by imipenem-resistant acinetobacter baumannii. typing of the isolates by three methods (antibiotyping, biotyping, and pulsed-field gel electrophoresis) revealed that two distinct strains were involved in the first 9 cases of the outbreak and that one of these strains, which had acquired a higher level of imipenem resistance as well as resistance to all aminoglycosides ... | 1994 | 7852555 |
| emergence of multidrug-resistant isolates of acinetobacter baumannii. | patterns of antimicrobial resistance during an outbreak of nosocomial infections caused by acinetobacter baumannii were studied. the medical records of all patients admitted to the hospital between february 1993 and february 1994 from whom a. baumannii was cultured were reviewed for demographic data, confirmation of the isolation report, admission date, date of first isolation of the organism, and antimicrobial use before and after the culture and susceptibility test results were obtained. the c ... | 1994 | 7856578 |
| detection of aac(6')-i genes in amikacin-resistant acinetobacter spp. by pcr. | the distribution of aac(6')-i genes in 62 strains of acinetobacter spp. resistant to amikacin, netilmicin, and tobramycin and susceptible to gentamicin, a phenotype compatible with synthesis of an aac(6')-i enzyme, was studied by pcr and by dna hybridization. both methods gave similar results. among the 51 acinetobacter baumannii strains, aac(6')-ib was found in 19 isolates and aac(6')-ih was found in the remaining strains. the aac(6')-ig gene was present in all 10 a. haemolyticus strains studie ... | 1994 | 7695286 |
| bacteremia due to acinetobacter species other than acinetobacter baumannii. | the objective of this study was to describe the clinical features, possible predisposing factors and treatment outcomes associated with bacteremia du to acinetobacter species other than acinetobacter baumannii. a review of laboratory and medical charts over a period of 18 months revealed 61 cases of bacteremia due to acinetobacter species other than a. baumannii occurring in 59 patients. six of these were considered not significant. fifty cases represented catheter-related bacteremia, one case w ... | 1994 | 7698833 |
| a comparative study of ribotyping and arbitrarily primed polymerase chain reaction for investigation of hospital outbreaks of acinetobacter baumannii infection. | arbitrarily primed polymerase chain reaction (ap-pcr) and ribotyping were compared in an investigation of an outbreak of acinetobacter baumannii infections. twenty-five clinical isolates shown previously by other criteria to belong to two different groups, and nine randomly selected a. baumannii clinical isolates from other hospitals were investigated. among the strains analysed, nine different ecori rrna gene restriction pattern fingerprints were observed. while similarity was detected between ... | 1994 | 7523678 |
| structure of the putative o10 antigen from acinetobacter baumannii. | a polysaccharide containing l-rhamnose, 2-acetamido-2-deoxy-d-glucose, and 2-acetamido-2-deoxy-d-mannose was obtained from an aqueous phenol extract of isolated cell walls from the reference strain for acinetobacter baumannii serogroup o10. by means of nmr studies and chemical degradations, the repeating unit of the polymer (the putative o10 antigen) was identified as a branched pentasaccharide of the structure shown. | 1994 | 7528093 |
| comparison of ribotyping and pulsed-field gel electrophoresis for molecular typing of acinetobacter isolates. | seventy-three isolates of the acinetobacter calcoaceticus-acinetobacter baumannii complex, including 26 isolates from 10 hospital outbreaks, were typed by ribotyping with ecori and clai and by pulsed-field gel electrophoresis (pfge) of genomic dna after digestion with apai. ribotyping with ecori distinguished 31 ribopatterns. digestion with clai generated another eight ribotypes. pfge, in contrast, identified 49 distinct patterns with seven variants. both methods detected all outbreak-related is ... | 1995 | 7542274 |
| risk factors for nosocomial colonization with multiresistant acinetobacter baumannii. | a six-month prospective survey was carried out in a university hospital to assess the incidence of acinetobacter baumannii cross-contamination and to identify risk factors for colonization. clinical isolates obtained during the study period were biotyped and genotyped by pulsed-field gel electrophoresis after apai macrorestriction of total dna. case-control univariate and multivariate analyses were performed to identify risk factors for acinetobacter baumannii colonization. one hundred forty-sev ... | 1995 | 7588840 |
| mutation in the gyra gene of quinolone-resistant clinical isolates of acinetobacter baumannii. | the gyra gene mutations associated with quinolone resistance were determined in 21 epidemiologically unrelated clinical isolates of acinetobacter baumannii. our studies highlight the conserved sequences in the quinolone resistance-determining region of the gyra gene from a. baumannii and other bacteria. all 15 isolates for which the mic of ciprofloxacin is > or = 4 micrograms/ml showed a change at ser-83 to leu. six strains for which the mic of ciprofloxacin is 1 microgram/ml did not show any ch ... | 1995 | 7625818 |
| in vitro activity of beta-lactamase inhibitors against clinical isolates of acinetobacter species. | acinetobacter is an important cause of nosocomial infections, and it is often resistant to many antibiotics. in a search for alternative agents, three beta-lactamase inhibitors (sulbactam, clavulanate, and tazobactam) and five beta-lactam antibiotics (imipenem, ceftazidime, ceftriaxone, cefotaxime, and piperacillin) were tested against 68 unique clinical isolates of acinetobacter species. minimum inhibitory concentrations were determined by a broth microdilution method. using temperature sensiti ... | 1995 | 7628190 |
| clinical and epidemiological investigations of acinetobacter genomospecies 3 in a neonatal intensive care unit. | a prospective study of acinetobacter isolates from a neonatal intensive care unit was performed for 24 months. fifty-six isolates were obtained from 21 patients, and another eight were obtained from environmental specimens. infection due to acinetobacter organisms was established for 16 patients, 6 with septicemia, 9 with pneumonia, and 1 with a wound infection. further investigations were performed with 38 representative isolates. twenty-nine isolates were identified as unnamed dna-dna hybridiz ... | 1995 | 7650188 |
| correlation of six methods for typing nosocomial isolates of acinetobacter baumannii. | a comparative study of biotyping, antimicrobial susceptibility, whole-cell protein analysis, plasmid analysis, pulsed-field gel electrophoresis of chromosomal dna and polymerase chain reaction with arbitrary primers of acinetobacter baumannii isolates from three large hospitals was performed to determine the best markers for epidemiological purposes. ninety-two isolates were included: 38 belonged to a previously described outbreak and 54 were randomly selected from sporadic cases of infection. b ... | 1995 | 7752211 |
| exposure of clinical isolates of acinetobacter baumannii and genospecies 3 to defibrinated human blood with and without added human 'natural' or (patient) immune antibodies. | fresh defibrinated blood (65% v/v) from 2 human donors failed to completely kill strains of acinetobacter baumannii and genospecies 3, representing four serovars each. although colony counts were reduced > or = 90%, there invariably occurred rebound growth following extended incubation. neither selected serovar-specific or isolate-homologous (following experimental bacteremia) rabbit immune sera nor three intravenously applicable igg preparations augmented the bactericidal activity of human bloo ... | 1995 | 7758361 |
| clinical and bacteriological features of relapsing shunt-associated meningitis due to acinetobacter baumannii. | a case is reported of a patient who developed acinetobacter baumannii meningitis ten days after a ventriculoperitoneal shunt had been placed for control of elevated intracranial pressure. intravenous antimicrobial therapy was instituted with imipenem and tobramycin after shunt removal and insertion of a ventriculostomy tube. following the placement of a ventriculoatrial shunt, clinical and microbiological relapse occurred that was eventually cured after complete removal of the ventricular draina ... | 1995 | 7758479 |
| nosocomial outbreak caused by a multiresistant clone of acinetobacter baumannii: results of the case-control and molecular epidemiologic investigations. | to describe a nosocomial outbreak caused by multiresistant acinetobacter baumannii. | 1995 | 7759825 |
| [respiratory infections in the surgical intensive care unit]. | the purpose of the study was to assess microbiological structure and the influence of the predisposing factors on frequency of lower respiratory tract infections. the study group consisted of 72 patients admitted to the intensive care unit between january and october 1994. we found that 27 pts. (39%) developed respiratory infections. the risk of an infection was much higher in the group with long (over 5 days) stay on icu, which required artificial ventilation as well as in the group of patients ... | 1995 | 7784613 |
| nosocomial acquisition of multiresistant acinetobacter baumannii: risk factors and prognosis. | to identify risk factors for and prognostic indicators of the nosocomial acquisition of multiresistant acinetobacter baumannii in an intensive care unit, we prospectively studied 40 patients: 13 who were infected with this organism and 27 who were colonized. isolates were identified by pulsed-field gel electrophoresis; the infected/colonized patients were compared with 348 noninfected, noncolonized patients by logistic regression analysis and with matched historical controls in a cohort study. t ... | 1995 | 7795075 |
| automated laser fluorescence analysis of randomly amplified polymorphic dna: a rapid method for investigating nosocomial transmission of acinetobacter baumannii. | a rapid method for genotyping acinetobacter baumannii based on pcr-fingerprinting with fluorescent primers was evaluated. automated laser fluorescence analysis (alfa) enabled on-line generation of high resolution dna-fingerprints during polyacrylamide gel electrophoresis of randomly amplified polymorphic dna (rapd) products. the results were in concordance with macro-restriction fragment patterns produced by pulse-field gel electrophoresis (pfge) of apai digests of chromosomal dna. rapd-alfa was ... | 1995 | 7473679 |
| [frequency of clinical isolation of glucose non-fermentative gram-negative rods and their susceptibilities to antibacterial agents]. | a comparison was made for frequencies of isolation o glucose non-fermentative gram-negative rods ((g)nf-gnr) from clinical specimens during a period from july, 1986 to june, 1987 (the first period) and that from january, 1994 to december, 1994 (the second period). also, minimum inhibitory concentrations of principal drugs were determined against these isolates. the obtained results are summarized as follows: 1. thirty four (34) species of (g)nf-gnr were found from 35,200 clinical specimens in th ... | 1995 | 7474336 |
| synergistic interaction between ofloxacin and cefotaxime against common clinical pathogens. | antimicrobial synergy resulting from combined antibiotic therapy is often important in the treatment of serious bacterial infections. to investigate the interactions between cefotaxime (ctx), desacetylcefotaxime (des), and ofloxacin (ofl), 247 recent clinical isolates were tested for in vitro susceptibility to each antibiotic alone by an agar dilution technique and retested with the various antibiotic combinations using a checkerboard protocol. fractional inhibitory concentrations were calculate ... | 1995 | 7499004 |
| identification and biotyping of acinetobacter spp. isolated in chilean hospitals. | two hundred and eighty-one isolates of acinetobacter spp. obtained from clinical specimens in hospitals from five chilean cities were identified to species level and biotyped. respiratory tract and wound secretions were the main sources of the isolates. acinetobacter baumannii was the most frequent species (96.8%), followed by acinetobacter genospecies 3 (2.8%). twelve different biotypes of a. baumannii strains were found of which biotypes 9, 8 and 6 were the most frequent. isolates of other bio ... | 1995 | 7499807 |
| genomic fingerprinting acinetobacter baumannii: amplification of multiple inter-repetitive extragenic palindromic sequences. | acinetobacter species are important nosocomial pathogens. a rapid and sensitive identification system, capable of providing strain identity at the genetic level, is required to identify outbreak strains and facilitate the early implementation of infection control procedures. repetitive extragenic palindromic (rep) elements, have been identified in numerous bacteria and these genomic sequences provide useful targets for dna amplification. a method for amplifying inter-rep dna sequences, rep-multi ... | 1995 | 7499819 |
| outbreak caused by two multi-resistant acinetobacter baumannii clones in a burns unit: emergence of resistance to imipenem. | since early 1992 an increased number of tobramycin- and imipenem-resistant acinetobacter spp. were observed causing colonization, wound infections, and bacteraemias in a burns and plastic surgery unit. this raised the question of whether this outbreak was caused by a single or by multiple acinetobacter spp. clones. to study this, 97 acinetobacter spp. isolates from clinical samples from different hospital units as well as isolates from the environment and the hands of the staff were characterize ... | 1995 | 7499820 |
| nosocomial bacteremia due to acinetobacter baumannii. clinical features, epidemiology, and predictors of mortality. | to study the possible predisposing factors, clinical features, molecular epidemiology, and factors affecting mortality associated with bacteremia due to acinetobacter baumannii, we reviewed 87 episodes of a. baumannii bacteremia occurring in 79 patients hospitalized at 2 university tertiary care centers and 4 community-based hospitals during a recent 18-month period. plasmid dna analysis and analysis of genomic dna with pulsed-field gel electrophoresis was performed to investigate possible epide ... | 1995 | 7500897 |
| molecular typing of acinetobacter baumannii-acinetobacter calcoaceticus complex isolates from endemic and epidemic nosocomial infections. | ribotype, biotype and resistance phenotype were used to characterize 37 acinetobacter baumannii-a. calcoaceticus complex isolates responsible for nosocomial infections in buenos aires. nineteen isolates were recovered from endemic infections at 2 hospitals and 18 represent an intensive care unit outbreak that occurred in a third hospital. by ribotyping isolates were classified into five different clones of a. baumannii biotype 2, 3 of a. baumannii biotype 9, and 3 of acinetobacter genospecies 13 ... | 1995 | 7867730 |
| acinetobacter baumannii outbreak isolates characterized by three typing methods. | forty-two strains of acinetobacter baumannii were isolated from 15 patients hospitalized in a french intensive care unit. an epidemiological study based on the typing of these isolates was carried out using biotyping, antibiotyping, and ribotyping to recognize the transmission of multiresistant strains by transfer of a patient from one hospital to another. fifteen strains from the outbreak (1 strain for each patient), five acinetobacter baumannii strains isolated before the outbreak in bellevue ... | 1995 | 8681988 |
| structural studies of the putative o-specific polysaccharide of acinetobacter baumannii o2 containing 3,6-dideoxy-3-n-(d-3-hydroxybutyryl)amino-d-galactose. | a polysaccharide containing d-galactose, 2-deoxy-2-n-acetylamino-d-galactose and 3,6-dideoxy-3-n-(d-3-hydroxybutyryl)amino-d-galactose, probably corresponding to the lipopolysaccharide side chain, was obtained from an aqueous phenol extract of isolated cell walls from acinetobacter baumannii strain o2. by means of nmr studies and chemical degradations, the repeating unit of the polymer was identified as a branched hexasaccharide of the structure shown, where fuc3n represents 3-amino-3,6-dideoxyg ... | 1995 | 8521857 |
| imipenem resistance in clinical isolates of proteus mirabilis associated with alterations in penicillin-binding proteins. | two strains of proteus mirabilis, ipr1 and ipr2, resistant to both imipenem and mecillinam, but susceptible to other beta-lactams were isolated from blood cultures of patients who had been treated with imipenem. strain ipr1 was isolated in the same sample as a p. mirabilis ips1 which was susceptible to imipenem and mecillinam. strains ipr1 and ipr2 did not produce a beta-lactamase and their outer membrane protein profiles were similar to those of ips1 and p. mirabilis atcc 29906. electrophoretic ... | 1995 | 8522463 |
| emergence of bacterial resistance to imipenem and ciprofloxacin in a university hospital. | we have continuously monitored the in-vitro activities of imipenem and ciprofloxacin against large numbers of non-fastidious clinical isolates. after eight years of use, 97-100% of enterobacteriaceae and acinetobacter baumannii remained susceptible to imipenem, but susceptibility of pseudomonas aeruginosa declined from 100% to 91%. after six years of use, 94%-100% of enterobacteriaceae (except providencia stuartii) remained susceptible to ciprofloxacin but susceptibility of p. stuartii, a. bauma ... | 1995 | 8522464 |
| microbiology of nosocomial sinusitis in intensive care unit patients. | we performed a 2-year microbiological study of specimens of sinus fluid recovered from patients with suspected nosocomial sinusitis in six intensive care units. over that period, 103 cases of sinusitis were documented by positive culture. the number of cases varied widely from one unit to another and was particularly high in the neurosurgical unit. cultures were often polymicrobial. the main pathogens were staphylococcus aureus, pseudomonas aeruginosa, acinetobacter baumannii and enterobacteriac ... | 1995 | 8522833 |