| molecular cloning of the structural gene for acinetobacter citrate synthase. | the structural gene for citrate synthase of acinetobacter anitratum has been cloned in escherichia coli in a form which expresses the enzyme. a library of ecori fragments of acinetobacter genomic dna was prepared in the vector lambda gt10, and clones were screened by hybridization with an e. coli citrate synthase clone under conditions of reduced stringency. a 6.5 kbp clone was obtained which was subcloned into pbr322, and shown to direct the formation of acinetobacter citrate synthase in e. col ... | 1986 | 3026391 |
| antibacterial activity of ofloxacin and its mode of action. | the antibacterial activity of ofloxacin against enterobacteriaceae, pseudomonas aeruginosa, haemophilus influenzae, branhamella catarrhalis, and neisseria gonorrhoeae was comparable to norfloxacin and enoxacin, and far exceeded the activity of pipemidic acid and nalidixic acid. the activity of ofloxacin was two to eight times less than that of ciprofloxacin. ofloxacin was more active against staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, acinetobacter spp., legionel ... | 1986 | 3028966 |
| [the medium for determining the gelatinase activity of non-fermenting gram-negative microorganisms]. | to determine the gelatinase activity of gram-negative nonfermenting microorganisms, a double-layer medium containing 2% of agar in the lower layer and 10-20% of gelatin in the upper layer has been developed. the medium ensures free access of atmospheric oxygen, possibility of rapid (24-42 h) determination of gelatinase activity at 22-25 degrees c, efficacy of the test. simultaneous determination of gelatin hydrolysis by 8-12 strains in one dish. | 1986 | 3030030 |
| cloning and genetic organization of the pca gene cluster from acinetobacter calcoaceticus. | the beta-ketoadipate pathway of acinetobacter calcoaceticus comprises two parallel metabolic branches. one branch, mediated by six enzymes encoded by the cat genes, converts catechol to succinate and acetyl coenzyme a (acetyl-coa); the other branch, catalyzed by products of the pca genes, converts protocatechuate to succinate and acetyl-coa by six metabolic reactions analogous or identical to those of the catechol sequence. we used the expression plasmid puc18 to construct expression libraries o ... | 1987 | 3036773 |
| outer membrane permeability of acinetobacter calcoaceticus mediates susceptibility to rat polymorphonuclear leukocyte granule contents. | growth of acinetobacter calcoaceticus on specific alkanes altered the outer membrane permeability of the organism, as indicated by a change in sensitivity to the antibiotic actinomycin d. as the carbon length of the alkane energy source decreased, outer membrane permeability and susceptibility to actinomycin d increased. concomitant with the increase in outer membrane permeability, a. calcoaceticus became more susceptible to the oxygen-independent antimicrobial activity of extracted contents fro ... | 1987 | 3040593 |
| bacterial nad(p)-independent quinate dehydrogenase is a quinoprotein. | acinetobacter calcoaceticus lmd 79.41 produced significant amounts of pyrrolo-quinoline quinone (pqq) in its culture medium when grown on quinic acid or shikimic acid. studies with lmd 79.41 and pqq- -mutants of this strain demonstrated that this organism contains an nad(p)-independent quinate dehydrogenase (qdh) (ec 1.1.99.-), catalyzing the first degradation step of these compounds, and that the enzyme contains pqq as a cofactor, i.e. is a quinoprotein. synthesis of qdh was induced by protocat ... | 1988 | 3044290 |
| ceftazidime combined with mecillinam: serum bactericidal titres compared with in-vitro synergy against gram-negative bacilli. | in a study of the possible interaction between mecillinam and ceftazidime against gram-negative bacilli, ten volunteers received on separate days: ceftazidime 20 mg/kg iv in 15 min, mecillinam 10 mg/kg iv in 15 min, or the combination. blood samples were obtained before and 1 and 6 h after the end of the infusion. ten strains each of klebsiella pneumoniae, serratia marcescens, citrobacter freundii, salmonella spp. and yersinia spp. and nine strains each of acinetobacter spp., and pseudomonas aer ... | 1988 | 3045067 |
| chorismate mutase:prephenate dehydratase from acinetobacter calcoaceticus. purification, properties and immunological cross-reactivity. | the bifunctional p protein (chorismate mutase: prephenate dehydratase) from acinetobacter calcoaceticus has been purified. it was homogeneous in polyacrylamide gels and was more than 95% pure on the basis of the immunostaining of purified p protein with the antibodies raised against the p protein. the native enzyme is a homodimer (mr = 91,000) composed of 45-kda subunits. a twofold increase in the native molecular mass of the p protein occurred in the presence of l-phenylalanine (inhibitor of bo ... | 1988 | 3046943 |
| a belgian multicentre in-vitro study of ofloxacin. | in a large multicentre study, the susceptibility of 2171 bacterial isolates to ofloxacin and other antibiotics has been examined by the kirby-bauer method. susceptibility to ofloxacin (breakpoint less than or equal to 2 mg/l) was observed in 99.2% of 1104 strains enterobacteriaceae, 88.9% of 217 pseudomonas aeruginosa, 93.6% of 31 other pseudomonas spp., 96.3% of 27 acinetobacter calcoaceticus, 99.2% of 118 haemophilus influenzae, 97.4% of 423 staphylococci, 79.4% of 107 streptococcus faecalis a ... | 1988 | 3053578 |
| comparison of mandelate dehydrogenases from various strains of acinetobacter calcoaceticus: similarity of natural and 'evolved' forms. | in previous work it had been shown that acinetobacter calcoaceticus wild-type strain ncib 8250 had only an l-mandelate deydrogenase but it could give rise to mutants that contained an evolved d-mandelate dehydrogenase; conversely, wild-type strain ebf 65/65 had only a d-mandelate dehydrogenase but gave rise to mutants that possessed an evolved l-mandelate dehydrogenase. several other wild-type strains of a. calcoaceticus have now been shown to grow on both enantiomers of mandelate. in every case ... | 1988 | 3053984 |
| [in vitro antibacterial activity of apalcillin on aerobic bacteria and the regression curve]. | minimal inhibitory concentrations (mics) of apalcillin (apl) were evaluated by agar dilution on 1,201 bacterial strains isolated simultaneously in four university hospitals; agar diffusion tests (disks apl: 75 micrograms) were performed on these strains to establish relationship between mic and zone diameters. for enterobacteriaceae naturally non beta-lactamase-producing (e. coli and p. mirabilis), mode mic was 0.5 microgram/ml; some acquired penicillinase-producing strains were only inhibited b ... | 1988 | 3054743 |
| [evolution of bacterial resistance to five aminoglycosides. a study of 3,354 strains isolated in a hospital milieu]. | the authors studied the susceptibility to 5 aminoglycosides (amikacin, dibekacin, gentamicin, netilmicin and tobramycin) of 3,354 strains isolated at the centre hospitalier sud in bordeaux during 1987. the results are compared to those obtained in 1984 on 2,818 strains. amikacin remains the most active aminoside against the enterobacteriaceae and acinetobacter; against pseudomonas, tobramycin has become the best one at that time, as well as netilmicin against staphylococcus aureus. evolution: no ... | 1988 | 3054744 |
| [microorganisms isolated from urinary tract infection and their beta-lactamase production and evaluation of clinical efficacy of sulperazone]. | clinical survey of microorganisms isolated from urinary tract infection (uti) was carried out at the four major hospitals in mie prefecture from may to july, 1987, and production of beta-lactamase of the microorganisms was determined by the acidimetric method, "beta-checker". among the total of 460 strains isolated from urine samples, 135 of gram positive cocci and 325 of gram negative rods were contained. sixty percent of the gram negative rods and 14% of gram positive cocci produced beta-lacta ... | 1988 | 3057834 |
| benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus. substrate specificities and inhibition studies. | the apparent km and maximum velocity values of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase ii from acinetobacter calcoaceticus were determined for a range of alcohols and aldehydes and the corresponding turnover numbers and specificity constants were calculated. benzyl alcohol was the most effective alcohol substrate for benzyl alcohol dehydrogenase. perillyl alcohol was the second most effective substrate, and was the only non-aromatic alcohol oxidized. the other substrates of b ... | 1988 | 3060114 |
| influence of antibiotic therapy on faecal carriage of p-fimbriated escherichia coli and other gram-negative bacteria in neonates. | the influence of previous antibiotic therapy on the aerobic faecal flora, including p-fimbriated escherichia coli, was studied in 953 neonates at discharge from 22 neonatal wards in sweden. antibiotics, mainly ampicillin (with or without gentamicin) or cefuroxime, had been received by 37% of the infants. treatment with ampicillin (with or without gentamicin) increased klebsiella/enterobacter and reduced esch. coli colonization. cephalosporin therapy (71% cefuroxime) reduced the frequency of colo ... | 1988 | 3060462 |
| flow cytometric screening and isolation of escherichia coli clones which express surface antigens of the oil-degrading microorganism acinetobacter calcoaceticus rag-1. | flow cytometry (fcm) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of escherichia coli clones containing a genomic library from the oil-degrading microorganism acinetobacter calcoaceticus rag-1 surface antigens. reconstruction experiments using mixed populations indicated that rag-1 cells could be clearly distinguished at a ratio of one rag-1 cell to 500 escherichia coli cells. using this technique two clones, wm143 and wm191, were isolated and shown ... | 1988 | 3061370 |
| peritonitis during continuous ambulatory peritoneal dialysis. treatment with pefloxacin: first results and pharmacokinetics. | | 1988 | 3065665 |
| acinetobacter calcoaceticus bacteremia 1984-1987. | | 1988 | 3068198 |
| comparative evaluation of the oxoid signal and roche septi-chek blood culture systems. | the oxoid signal blood culture system (oxoid usa, inc., columbia, md.) was compared with the roche septi-chek system (roche diagnostics, div. hoffmann-la roche inc., nutley, n.j.), with the latter consisting of a tryptic soy broth (r-tsb) bottle with an attached agar slide unit and a columbia broth bottle. a total of 5,034 cultures with equal volumes of blood in each bottle were processed. overall, more organisms were recovered in the r-tsb bottle than in the signal bottle, with significantly mo ... | 1988 | 3068248 |
| comparative in vitro activity of lomefloxacin and other antimicrobials against 597 microorganisms causing bacteremia. | the in vitro activity of lomefloxacin, a new fluorinated quinolone antimicrobial, was compared to that of enoxacin, norfloxacin, ofloxacin, imipenem, cefotaxime, ceftazidime, and tobramycin against 597 microorganisms isolated from bacteremic patients at a university hospital. overall, lomefloxacin had activity similar to that of enoxacin and norfloxacin but less than that of ofloxacin. lomefloxacin had excellent activity against members of the family enterobacteriaceae and haemophilus influenzae ... | 1988 | 3071448 |
| cloning and expression of pca genes from pseudomonas putida in escherichia coli. | beta-ketoadipate elicits expression of five structural pca genes encoding enzymes that catalyse consecutive reactions in the utilization of protocatechuate by pseudomonas putida. three derivatives of p. putida prs2000 were obtained, each carrying a single copy of tn5 dna inserted into a separate region of the genome and preventing expression of different sets of pca genes. selection of tn5 in or near the pca genes in these derivatives was used to clone four structural pca genes and to enable the ... | 1988 | 3076176 |
| antibiogram, biotyping and plasmid profile analysis of acinetobacter calcoaceticus var. anitratus. | antibiogram, biotyping, and plasmid profile analysis were studied on 115 isolates (40 nosocomial and 75 community strains) of acinetobacter calcoaceticus var. anitratus isolated from clinical specimens. by disk diffusion method, we found 14 antimicrobial susceptibility patterns. among them, the gentamicin-resistant patterns were recovered more frequently from nosocomial origin than from community-acquired origin. fifteen patterns of biocodes were found by using gni card of automicrobic system (v ... | 1988 | 3076859 |
| [heterogeneity of microbial flora responsible for respiratory infections in aids]. | | 1988 | 3077955 |
| separation of isoenzymes of citrate synthase and isocitrate dehydrogenase by fast protein liquid chromatography. | fast protein liquid chromatography (fplc) has been shown to be a rapid and effective method of separating isoenzymes of citrate synthase and isocitrate dehydrogenase in extracts of pseudomonas aeruginosa and acinetobacter calcoaceticus. the advantages of fplc over conventional methods of fractionation are discussed and it is suggested that this may be a valuable and more general technique for isoenzyme resolution. | 1986 | 3081366 |
| organization of citric acid cycle enzymes into a multienzyme cluster. | the possibility that some of the enzymes of the citric acid cycle may be loosely associated into a multienzyme cluster has been investigated using extracts prepared by gentle disruption of cells. gel filtration and sucrose density gradient centrifugation have shown that five sequential enzymes of the cycle specifically associate into a cluster: fumarase, malate dehydrogenase, citrate synthase, aconitase and isocitrate dehydrogenase. ultrasonication destroys the abilities of the enzymes to associ ... | 1986 | 3086126 |
| in vitro activity of brl 36650, a new penicillin. | compared with four beta-lactam antibiotics and amikacin, brl 36650 and bmy-28142 were the most active against 509 enterobacteriaceae, including cefotaxime-resistant isolates and resistant laboratory mutants. brl 36650 was more active than aztreonam, ceftazidime, and bmy-28142 against acinetobacter spp., pseudomonas aeruginosa, and other pseudomonas spp.; all strains were inhibited by 2 micrograms/ml or less. | 1986 | 3087281 |
| synergistic activity of amikacin with aztreonam against pseudomonas aeruginosa and other gram-negative organisms. | the effects of amikacin in combination with aztreonam were tested on 100 strains of gram-negative bacteria by means of the microtiter checkerboard system. eighty-one strains were pseudomonas aeruginosa, eight were enterobacter species, seven were acinetobacter species, two were alcaligenes species, one was a citrobacter organism, and one was pseudomonas stutzeri. all strains had a minimal inhibitory concentration (mic) to aztreonam between 4 and 256 micrograms/ml (mic50 = 16 micrograms/ml) and a ... | 1986 | 3087621 |
| microplate technique to determine hemolytic activity for routine typing of listeria strains. | because the hemolysis produced by listeria monocytogenes and listeria seeligeri on blood agar is frequently difficult to interpret, we developed a microplate technique for the routine determination of hemolytic activity with erythrocyte suspensions. this microtechnique is a simple and reliable test for distinguishing clearly between hemolytic and nonhemolytic strains and could be used instead of the camp (christie-atkins-munch-petersen) test with staphylococcus aureus in the routine typing of li ... | 1986 | 3088037 |
| in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics. | a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ... | 1986 | 3088346 |
| enhanced emulsan production in mutants of acinetobacter calcoaceticus rag-1 selected for resistance to cetyltrimethylammonium bromide. | mutants of acinetobacter calcoaceticus rag-1 that produced elevated levels of the polymeric bioemulsifier emulsan were isolated on the basis of their resistance to the cationic surfactant cetyltrimethylammonium bromide (ctab). such mutants showed maximum enhancement in both overall yield and specific productivity of some two- to threefold over that of the wild type. in addition, the effect was also observed in a resting cell system in the presence of chloramphenicol, indicating that the mutation ... | 1986 | 3089157 |
| [relation of growth type in soft-agar to the biological properties of fresh isolates of strains of acinetobacter anitrautus]. | | 1986 | 3090167 |
| synthesis and biological activity of benzothiazolo[3,2-a]quinolone antibacterial agents. | a new class of heterocyclic compounds with potent antibacterial activity, namely, 2-substituted amino-3-fluoro-5,12-dihydro-5-oxobenzothiazolo[3, 2-a]quinoline-6-carboxylic acids, is described. the compounds are conformationally restricted analogues of 7-substituted amino-6-fluoro-1-aryl-1, 4-dihydro-4-oxoquinoline-3-carboxylic acids. compounds 7 and 10, having a 4-methylpiperazinyl and a piperazinyl substitution at the 2-position, respectively, possess in vitro antibacterial activities comparab ... | 1986 | 3090265 |
| [endocarditis on cardiac pacemaker endocavitary electrodes. apropos of 7 cases]. | the authors report 7 cases of endocarditis on cardiac pacing catheters observed out of a total of 2 950 primary implantations and 1 600 pacemaker replacements. this is a rare condition (0.15%) but carries a poor prognosis as it usually occurs in elderly patients and demands aggressive management. the presence of multiple pacing catheters and surgical contamination due to manipulation of the pulse generator (reimplantation, pacemaker replacement) are predisposing factors. the infecting organism i ... | 1986 | 3090966 |
| [in vitro activity of new quinolones against nonfermenters and references to sensitivity tests]. | studies of cross resistance between norfloxacin, ofloxacin, enoxacin and ciprofloxacin using 599 strains of non-fermentative gram-negative rods (297 pseudomonas spp. and 302 acinetobacter spp.) resulted in nearly identical minimal inhibitory concentrations of norfloxacin and enoxacin comparing mic values, in most ofloxacin was one to four dilution steps superior to enoxacin, and ciprofloxacin was one to four dilution steps superior to ofloxacin. there was not much difference in mics when species ... | 1986 | 3093387 |
| [in-vitro activity of cefonicid on hospital bacteria. regression line and proposal for critical values]. | antimicrobial activity of cefonicid, a new second generation cephalosporin, against 315 hospital isolates (4th trimester 1984) was investigated. e. coli and proteus mirabilis were the most susceptible species. all e. coli strains except one were inhibited at 8 mg/l (modal mic: 0.5); mics of all indole + proteus were 8 mg/l (modal mic: 0.06). another group was moderately susceptible: mics of klebsiella and citrobacter ranged from 0.12 to 128 mg/l, but mics of 50% of these strains were less than o ... | 1986 | 3095773 |
| residual effect of storage in an elevated carbon dioxide atmosphere on the microbial flora of rock cod (sebastes spp.). | a residual inhibitory effect on microbial growth due to modified-atmosphere (ma) storage (ma, 80% co2-20% air) was demonstrated for rock cod fillets stored in ma and transferred to air at 4 degrees c. results of measurements of co2 concentrations of the fillets suggested that the residual effect after transfer from ma to air was not due to retention of co2 at the surface of the fillets but was probably due to the microbial ecology of the system. lactobacillus spp. and tan alteromonas spp. (tan) ... | 1986 | 3096204 |
| in vitro activity of sch-34343, a new penam, and other antimicrobial agents against clinical isolates from cancer patients. | the in vitro activity of sch-34343, a new penam antibiotic, was tested against gram-positive and gram-negative isolates from cancer patients, and compared to that of 7 other antimicrobial agents. sch-34343 was extremely active against the enterobacteriaceae with mic90 ranging from 0.39 to 6.25 micrograms/ml for citrobacter spp., enterobacter spp., escherichia coli, klebsiella spp., proteus spp. and serratia marcescens. it was less active against acinetobacter spp. (mic90 6.25-12.5 micrograms/ml) ... | 1986 | 3100147 |
| determination of endotoxins on hypodermic needles by means of a chromogenic limulus amoebocyte lysate assay. development of a test model. | a test model was developed in order to describe the determination of endotoxins on hypodermic needles in a reliable and reproducible manner. as in all in-vitro experiments one has to be very careful about extrapolating data to in-vivo situations. however by choosing a hydrophilic (salmonella typhimurium atcc 14028) and a hydrophobic bacterium (acinetobacter calcoaceticus var. anitratus rr 8212/113), one could hope to obtain a quite representative idea about the extraction of contaminating gram-n ... | 1986 | 3101323 |
| antibacterial properties of imipenem with special reference to the activity against methicillin-resistant staphylococci, cefotaxime-resistant enterobacteriaceae and pseudomonas aeruginosa. | imipenem was examined with standardized agar dilution procedures against a wide range of bacteria. geometric mean mics against the genera escherichia, klebsiella, enterobacter, citrobacter and serratia were 0.1-0.4 mg/l, and proteus and providencia spp. were inhibited by 0.25-4 mg/l. acinetobacter calcoaceticus var. anitratum strains were inhibited by concentrations ranging from 0.12-0.5 mg/l. methicillin-susceptible staphylococci were highly susceptible to the drug (mics: less than or equal to ... | 1986 | 3102452 |
| emergence of resistant bacterial strains during treatment of infections in the respiratory tract. | in order to investigate the frequency of the emergence of resistance during treatment, 1,403 episodes of lower respiratory infection were studied in a general hospital with three departments of chest medicine in a period of four years. in 650 episodes the pathogen was isolated and in 82 of those failure of therapy was accompanied by emergence of resistance to the agent used. factors associated with this phenomenon were: intensive care, tracheostomy, involvement of pseudomonas aeruginosa, enterob ... | 1986 | 3103208 |
| in vitro activity of amifloxacin (win 49,375) compared with those of ciprofloxacin and ofloxacin. | the in vitro activity of the novel fluoroquinolone derivative, amifloxacin (win 49,375), was compared with the activities of ciprofloxacin and ofloxacin. a total of 500 clinical isolates of gram-negative and gram-positive bacteria were included, and the minimal inhibitory concentration (mic) was determined by an agar dilution method. all drugs were highly active against enterobacteriaceae, but ciprofloxacin showed the highest activity on a weight-for-weight basis (mic 90% less than or equal to 0 ... | 1987 | 3105246 |
| in vitro evaluation of tigemonam, a novel oral monobactam. | tigemonam, a novel, orally administered monobactam, exhibited potent and specific activity in vitro against members of the family enterobacteriaceae, haemophilus influenzae, and neisseria gonorrhoeae. its activity was variable to poor against gram-positive bacteria, acinetobacter spp., pseudomonas aeruginosa, and anaerobes. within its spectrum of activity, tigemonam was far superior to oral antibiotics currently available, including amoxicillin-clavulanic acid, cefaclor, and trimethoprim-sulfame ... | 1987 | 3105448 |
| serum bactericidal activity of ciprofloxacin and ofloxacin in volunteers. | the serum bactericidal activity of two newer quinolones, ciprofloxacin and ofloxacin, against 206 clinical bacterial isolates was determined in six male volunteers after oral administration of either 500 mg of ciprofloxacin or 200 mg of ofloxacin respectively. the highest bactericidal titers were achieved against enterobacteriaceae 1 h after ciprofloxacin administration, ranging from 1:121 for indole-positive proteus species to 1:30 for serratia spp. ofloxacin generated lower titers, ranging fro ... | 1987 | 3106034 |
| dynamics of ceftazidime-pefloxacin interaction shown by a new killing curve-chequerboard method. | since in-vitro methods for studying drug interactions are difficult to evaluate and different results occur with the chequerboard (isobolograms-fic index) and killing curve methods, a new approach associating these two methods was used to study the ceftazidime-pefloxacin interaction. in 64 tubes in a chequerboard pattern, viable bacteria were counted at 0, 2.5, 5 and 24 h, by a microdilution method and a multiple inoculator replicating method. the bacterial inoculum consisted of 5 x 10(6)-10(7) ... | 1987 | 3106301 |
| bacterial colonization and occurrence of legionella pneumophila in warm and cold water, in faucet aerators, and in drains of hospitals. | warm and cold water as well as water from wash basin drains and faucet aerators was examined to determine the number of viable and dead bacteria by culture and by staining and to establish the spectrum of species with special consideration of legionella pneumophila. the relation between the number of legionella pneumophila, the temperature, and the iron content of the water was determined in three separate warm water systems. high colony counts (up to 8.9 x 10(5) colony-forming units), were dete ... | 1986 | 3107260 |
| in vitro activity of ro 23-6240 (am-833): a new fluoroquinolone. | the in vitro activities of ro 23-6240 (am-833) and four comparative fluoroquinolones were studied. minimal inhibitory concentrations (mics) of ro 23-6240, which inhibited at least 90% of strains, were less than or equal to 0.03-1 microgram/ml for staphylococcus aureus, staphylococcus epidermidis, enterobacteriaceae, acinetobacter anitratus aeromonas hydrophila, branhamella catarrhalis, and haemophilus influenzae, 2-8 micrograms/ml for staphylococcus saprophyticus, streptococci, diphtheroids, and ... | 1987 | 3107872 |
| analysis of the microbiological particulates in municipal drinking-water by scanning electron microscopy/x-ray energy spectroscopy. | scanning electron microscopy and x-ray energy spectroscopy (sem/xes) were used to survey the biological and nonbiological particles in two different municipal drinking-water systems. microbiological particles could be differentiated from non-biological by their qualitative elemental compositions and this information was used as the basis for an automated detection scheme. automated sem/xes analyses were used to demonstrate microbiological differences between well-water and surface-water in distr ... | 1987 | 3109154 |
| combined resistance to quinolones and beta-lactams after in vitro transfer on single drugs. | single and combined resistance to quinolones and beta-lactams was determined after serial transfers of 18 selected strains of pseudomonas aeruginosa (5), acinetobacter calcoaceticus (3) and beta-lactamase producing enterobacterial strains (10), in broth dilutions of 4 quinolones and 8 beta-lactams. two definitions for resistance were used: (i) 8-fold mic increase and stability of acquired resistance after five transfers in drug-free broth; and (ii) 8-fold mic increase over the breakpoint level. ... | 1987 | 3109816 |
| in vitro activity of ro 19-5247 (t-2525) and interpretive criteria for disk diffusion susceptibility testing. | the activity of ro 19-5247 (the active metabolite of the oral cephalosporin ro 19-5248 [t-2588]) was compared with that of five orally active agents against a total of 331 bacterial strains. ro 19-5247 was more active in vitro than amoxicillin, amoxicillin-clavulanate, cefaclor, cefuroxime, and cephalexin against members of the family enterobacteriaceae. amoxicillin-clavulanate and amoxicillin overall were more active than the other four agents against staphylococci. ro 19-5247, amoxicillin-clav ... | 1987 | 3112176 |
| [study of the sensitivity to pipemidic acid, pefloxacin and norfloxacin of 444 bacterial strains isolated at a general hospital]. | after one year of use of pefloxacin in the intensive care unit, medicine unit and surgery unit and the following recent commercialization without prescription of norfloxacin, we studied for a period of two months (april and may 1986) the susceptibility to pipemidic acid, to pefloxacin and norfloxacin of 444 bacteria strains isolated obtained from clinical specimens. the antibiotic susceptibility is determined by disk diffusion test. we obtained the following results: susceptible methicillin stap ... | 1987 | 3112705 |
| [effect of aztreonam on the fecal aerobic flora in children]. | aztreonam, a new monobactam, has a spectrum limited to gram negative aerobic bacilli. to evaluate its effect after parenteral administration on aerobic stool flora (gram negative bacilli, streptococci d, staphylococci, candida), quantitative cultures used serial dilution of stools twice a week on selective media. for gram negative bacilli, agar dishes containing aztreonam or not were used. colonies of different morphologies were counted. representatives of each morphological type were then picke ... | 1987 | 3112714 |
| adhesion of bacteria from mixed cell suspension to solid surfaces. | the attachment of four species of bacteria to solid surfaces was investigated to determine whether the attachment of one species of bacterium could be influenced by the presence of other attaching or attached species. three types of experiment were done: attachment of bacteria from suspensions containing two species (termed "simultaneous attachment") was compared to attachment of each species in pure culture, the attachment of one species of bacterium to surfaces already colonized by a second sp ... | 1987 | 3115220 |
| effect of chlorhexidine/edta/tris against bacterial isolates from clinical specimens. | chlorhexidine/edta/tris was more active compared with chlorhexidine against the following species of organisms: acinetobacter species, citrobacter species, enterobacter species, escherichia coli, proteus mirabilis, providence species, pseudomonas species, staphylococcus aureus, staphylococcus epidermidis and streptococcus faecalis, when tested in isosensitest agar. the addition of 20% serum to the medium reduced its effectiveness mainly against providence species, proteus species, and streptococ ... | 1987 | 3116368 |
| [acinetobacter and streptococcus faecalis septicemia with recurring coma, treated with ceftriaxone]. | | 1987 | 3116632 |
| [epidemiologic studies of the microbial colonization of severely burned patients]. | bacteria isolated between 1/1/1983 and 12/31/1984 from the burns of 122 patients in a burns unit at the ruhr-universität bochum were studied. grampositive bacteria were predominant in colonizing the burn wounds (62.5% of all strains isolated). in the patients with more than 40% of total body surface area (tbsa) burn, isolation of staph aureus was most frequent. the exogenous colonization rate with staph. aureus was 86%. coagulase negative staphylococci were identified in 89.6% of all patients (7 ... | 1987 | 3116791 |
| serum bactericidal activity and postantibiotic effect in serum of patients with urinary tract infection receiving high-dose amikacin. | ten patients received a 30-min infusion of amikacin (30 mg/kg) on day 1 and 15 mg/kg on day 2. mean serum creatinine was 1.1 +/- 0.3 (standard deviation) mg/dl before and 1.0 +/- 0.3 mg/dl 3 days after the second infusion. mean serum amikacin concentrations before, at the end of infusion, and 1, 6, 12, and 24 h after 30 and 15 mg/kg were 0, 157, 79, 31, 16, 5, 5, 85, 51, 19, 12, and 5 mg/liter, respectively. five strains each of staphylococcus aureus, staphylococcus epidermidis susceptible and r ... | 1987 | 3116918 |
| comparative in vitro activity of ro 23-6240 (fleroxacin), a new 4-quinolone derivative. | the in vitro activity of ro 23-6240 was compared with that of norfloxacin, ofloxacin and ciprofloxacin as well as four other antimicrobial agents against 345 recent clinical isolates. the mics of ro 23-6240 against enterobacteriaceae and acinetobacter anitratum was less than or equal to 0.5 mg/l. at the same concentration of the compound 90% of staphylococci were inhibited. against enterococcus faecalis and pseudomonas aeruginosa ro 23-6240 proved less active, having mic90 values of 4.0 mg/l and ... | 1987 | 3117538 |
| a rapid method for measuring protease activity in milk using radiolabeled casein. | a rapid means to detect the presence of protease activity in raw milk could be useful in predicting keeping ability of products made from that milk. a 30-min assay has been developed and compared with three other methods of detecting protease. casein, [methyl-14c]-methylated-alpha was purchased from a radioisotope supplier. concentrations of substrate from 2 to 20 nci gave counts per minute, which increased linearly when counted with the charm analyzer. there was not a significant difference in ... | 1987 | 3117854 |
| ro23-6240, a new orally absorbed quinolone: in vitro comparison with other broad-spectrum oral antimicrobial agents and imipenem. | a total of 626 clinical isolates were tested for their susceptibility to ro23-6240 and other broad-spectrum antimicrobial agents. ro23-6240 showed good activity against strains of enterobacteriaceae, acinetobacter, and p. aeruginosa. ro23-6240 mic90s ranged from 0.032 to 4 micrograms/ml for these strains. ro23-6240 also showed good activity against staphylococci, both methicillin-susceptible and -resistant strains. the activity in vitro of ro23-6240 was comparable with that of norfloxacin and of ... | 1987 | 3121244 |
| solubilization of bacterial cells in organic solvents via reverse micelles and microemulsions. | | 1987 | 3124689 |
| susceptibility of 310 nonfermentative gram-negative bacteria to aztreonam, carumonam, ciprofloxacin, ofloxacin and fleroxacin. | the susceptibility of 310 mainly clinically isolated gram-negative nonfermenters to aztreonam, carumonam, ciprofloxacin, ofloxacin and fleroxacin was determined by agar dilution. all 3 quinolones were very active, with mic90 ranges (micrograms/ml) against all organisms ranging between 0.25 and 8 for ciprofloxacin, and 0.25 and 16.0 for ofloxacin and fleroxacin, respectively. aztreonam and carumonam showed less activity than the quinolones on a weight-for-weight basis, with mic90 values ranging f ... | 1988 | 3127127 |
| [effect of the slime from acinetobacter calcoaceticus on the function of phagocytic cells]. | | 1987 | 3127500 |
| [in vitro activity of ciprofloxacin against multiresistant microorganisms isolated from cases of hospital infection]. | | 1987 | 3129763 |
| [action of ultraviolet radiation on hydrocarbon-oxidizing bacteria]. | | 1987 | 3130549 |
| [activity of selected cephalosporin antibiotics against clinical strains of gram-negative bacteria]. | | 1987 | 3131750 |
| in vitro activity of temafloxacin, a new difluoro quinolone antimicrobial agent. | temafloxacin, a new difluoro quinolone, inhibited the majority of enterobacteriaceae at less than or equal to 1 microgram/ml. it was 4-8-fold less active than ciprofloxacin and 2-fold less active than ofloxacin. cefotaxime and imipenem-resistant isolates such as enterobacter cloacae, citrobacter freundii, pseudomonas aeruginosa, and acinetobacter spp. were inhibited. temafloxacin inhibited neisseria, branhamella, and haemophilus species at less than 0.25 microgram/ml. methicillin-susceptible and ... | 1988 | 3132380 |
| in vitro evaluation of e1040, a new cephalosporin with potent antipseudomonal activity. | e1040 is a new parenteral cephalosporin with a broad antibacterial spectrum and potent antipseudomonal activity. the compound was four- to eightfold more active than ceftazidime and cefsulodin against pseudomonas aeruginosa (mic of e1040 for 90% of strains tested [mic90], 3.13 micrograms/ml). e1040 also showed a potent activity against other glucose-nonfermentative rods, including acinetobacter species. the activities of e1040 against most species of the family enterobacteriaceae were roughly co ... | 1988 | 3134847 |
| [comparative activity of habekacin and 4 other aminoglycosides against gram-negative bacilli. evolution of resistance]. | the activity of 5 aminoglycosides compounds (habekacin, amikacin, gentamicin, netilmicin and tobramycin) was studied by an agar dilution method, against 235 strains of enterobacteriaceae and 146 other gram negative bacilli. 79 to 98% of susceptible strains were observed, according to the aminoglycoside compound. habekacin and amikacin were the most effective, specially against the more frequently resistant bacteria: enterobacter, serratia, hafnia, acinetobacter, pseudomonas aeruginosa. in compar ... | 1988 | 3136424 |
| comparative in vitro activity of the new oral cephalosporin cefixime. | cefixime was 8 to 10 times more active than cefaclor and augmentin against isolates of escherichia coli, klebsiella pneumoniae and salmonella typhi, mic90 values ranging from 0.06 to 0.25 micrograms/ml. however, none of these three drugs was particularly active against isolates of more resistant gram-negative bacilli such as enterobacter, serratia, citrobacter, acinetobacter, providencia and achromobacter spp. the lowest mic values for gram-negative bacilli were seen with ciprofloxacin, except f ... | 1988 | 3137053 |
| temocillin: comparative in vitro activity against gram-negative clinical isolates. | | 1988 | 3137759 |
| cometabolism of polychlorinated biphenyls: enhanced transformation of aroclor 1254 by growing bacterial cells. | acinetobacter sp. strain p6 and a soil isolate, arthrobacter sp. strain b1b, were tested for their ability to transform aroclor 1254 as washed resting cells and as growing cells with biphenyl as the substrate. growing cells were far superior to resting-cell suspensions in terms of total polychlorinated biphenyl (pcb) transformation, transformation of specific pcb congeners, and diversity of congeners that were attacked. growing cells of acinetobacter sp. strain p6 and arthrobacter sp. strain b1b ... | 1988 | 3140725 |
| in vitro studies of fleroxacin (ro 23-6240), a new trifluorinated quinolone derivative. | the in vitro activity of fleroxacin (ro 23-6240) against 441 bacterial isolates was compared with those of ciprofloxacin, ofloxacin, amoxycillin, cefadroxil, cefuroxime and tobramycin. an agar dilution method was used for the determination of minimal inhibitory concentrations (mics). ciprofloxacin showed the highest activity against the enterobacteriaceae, 95% of the isolates were inhibited by 0.06 mg/l, but fleroxacin and ofloxacin were also highly active (mic 90% = 0.5 and 0.25 mg/l, respectiv ... | 1988 | 3141116 |
| endemic acinetobacter anitratus in a surgical intensive care unit: mechanical ventilators as reservoir. | colonization and infection with an endemic multiresistant strain of acinetobacter calcoaceticus variety anitratus had been observed in the surgical intensive care unit of a university hospital since 1982. an outbreak of infection with this endemic, multiresistant acinetobacter anitratus strain occurred between january and september, 1984. after initial attempts at identification of environmental reservoirs had been unsuccessful, risk factors for the acquisition of acinetobacter anitratus were in ... | 1988 | 3141154 |
| postoperative prophylaxis with norfloxacin in patients requiring bladder catheters. | the effect of once daily doses of 200 mg oral norfloxacin on the occurrence of catheter-associated bacteriuria (greater than 1000 cfu/ml) and pyuria was studied in 105 post-operative gynaecologic patients. norfloxacin was given from the second day after surgery until catheter removal. bacteriuria developed in 32 of 51 (63%) control patients compared to 8 of 54 (15%) patients receiving norfloxacin (p less than 0.001). pyuria was present in 22 of 51 (43%) control subjects versus only 3 of 54 (5%) ... | 1988 | 3141155 |
| [serine proteinase with lytic properties]. | a thermophilic bacillus licheniformis strain can synthesize extracellular serine proteinase, which is similar to subtilisin of the carlsberg type in its amino acid composition, the specificity of its action, the character of its inhibition by certain compounds and immunochemical properties, but differs from the latter in its greater thermostability and in the ability to cause lysis of gram-negative bacteria and yeast living cells. | 1988 | 3141748 |
| [in vitro antibacterial activity of cefpirome in combination with 4 aminoglycosides and 2 fluoroquinolones]. | combinations of cefpirome with aminoglycosides (gentamicin, tobramycin, netilmicin, amikacin) and of cefpirome with fluorinated quinolones (pefloxacin, ofloxacin) were tested against 20 enterobacteriaceae, 6 pseudomonas aeruginosa, 6 acinetobacter calcoaceticus, 6 group d streptococci and 6 staphylococcus aureus strains, susceptible, intermediate or resistant with a low level to these different compounds. activity of combinations was evaluated using a broth microdilution checkerboard method and ... | 1988 | 3141888 |
| differentiation of some gram-negative glucose nonfermenting bacteria using miniaturized carbon sources assimilation tests. | in water and soil the gram-negative nonfermenting bacteria play an important role in the biological mineralization process. to improve the methods for species differentiation of these heterogenous bacterial group, a total of 481 reference strains of gram-negative glucose nonfermenting bacteria belonging to the genera pseudomonas, alcaligenes, bordetella, agrobacterium, moraxella, acinetobacter, flavobacterium and some cdc groups have been investigated for their ability to utilize 42 different ca ... | 1988 | 3142164 |
| comparative in vitro activity of the new difluoro-quinolone temafloxacin (a-62254) against bacterial isolates from cancer patients. | the in vitro activity of temafloxacin, a new difluoro quinolone agent, against 725 bacterial isolates representing 32 species was evaluated in comparison with that of ciprofloxacin. temafloxacin inhibited the majority of enterobacteriaceae isolates at a concentration of less than or equal to 0.5 microgram/ml. it was also extremely active against acinetobacter spp. and aeromonas hydrophila. its activity was 2-8 fold less than that of ciprofloxacin against most gram-negative isolates. methicillin- ... | 1988 | 3143585 |
| in-vitro activity of fleroxacin. | fleroxacin is a new synthetic fluorinated quinolone antimicrobial agent. the in-vitro activity of fleroxacin and five comparative quinolones against 541 clinical isolates was studied. minimum inhibitory concentrations (mic90) of fleroxacin were less than or equal to 2.0 mg/l for enterobacteriaceae, less than or equal to 8.0 mg/l for pseudomonas aeruginosa and 1.0 mg/l for acinetobacter calcoaceficis and 8 mg/l for streptococci. the activity of fleroxacin was comparable with that of ofloxacin, pe ... | 1988 | 3144544 |
| [serological typing of acinetobacter calcoaceticus subspecies anitratus strains by soft-agar technique]. | | 1988 | 3146608 |
| in vitro evaluation of ceftibuten (7432-s, sch 39720), a novel orally administered cephalosporin. | 7432-s (sch 39720) was the most active beta-lactam tested against the enterobacteriaceae, inhibiting 92% of strains at less than or equal to 8.0 micrograms/ml compared to 82%, 65% and 39% of strains inhibited by cefixime, cefuroxime and cefaclor, respectively. 7432-s was also very effective against haemophilus influenzae (mic90, less than or equal to 0.25 microgram/ml), branhamella catarrhalis (mic90, 4.0 micrograms/ml) and neisseria meningitidis (mic90, less than or equal to 0.25 microgram/ml). ... | 1988 | 3147148 |
| in vitro activity of fleroxacin (ro23-6240), a new fluorinated 4-quinolone against isolates from cancer patients. | the in vitro activity of fleroxacin (ro23-6240; am 833), a new fluorinated 4-quinolone, was compared to that of ciprofloxacin, enoxacin and a-56620, against 747 isolates from cancer patients. fleroxacin inhibited more than 90% of enterobacteriacea isolates at a concentration of less than or equal to 0.25 micrograms/ml. it was also extremely active against aeromonas hydrophila and haemophilus influenzae isolates with mic90 values of 0.12 and 0.06 micrograms/ml, respectively. the mic90 for acineto ... | 1988 | 3149568 |
| [a medium for determining the mobility of non-fermenting gram-negative microorganisms]. | | 1988 | 3149615 |
| leukopenic and lethal effects of slime from acinetobacter calcoaceticus. | | 1988 | 3150412 |
| [study of the horizontal transfer of mercury resistance genes in natural populations of bacteria using antibodies to mercury reductases]. | mercury resistant soil and intestinal bacteria were isolated from different mercury deposit areas of the ussr. mercury reductases from all gram negative bacteria studied (pseudomonas, acinetobacter and enterobacterial species) with a single exception (flavobacterium sp.) were immunologically cross reactive. two immunological types of mercury reductases were found among gram positive bacteria (bacillus, staphylococcus and coryneform species). further subdivisions were done by "spur" formation tes ... | 1988 | 3150770 |
| comparative activity in vitro of ticarcillin and ticarcillin plus clavulanic acid against enterobacteriaceae, pseudomonas aeruginosa and acinetobacter calcoaceticus. | | 1987 | 3151358 |
| comparative evaluation of recently developed quinolone compounds--with a note on the frequency of resistant mutants. | the antibacterial activity of the new quinolone compounds enoxacin, norfloxacin, ofloxacin and ciprofloxacin was evaluated in 300 enterobacteriaceae, 50 pseudomonas aeruginosa, 30 acinetobacter spp., 15 haemophilus influenzae, 50 streptococcus faecalis, and 70 staphylococcus aureus isolates and compared to that of nalidixic acid, gentamicin and various beta-lactam compounds. moreover, the rate of spontaneous mutants resistant to quinolone compounds was evaluated. in concentrations only insignifi ... | 1985 | 3156025 |
| interaction between ciprofloxacin and tobramycin or azlocillin against multiresistant strains of acinetobacter anitratum in vitro. | | 1985 | 3159571 |
| comparative in-vitro activity of ciprofloxacin against non-fermenters. | the in-vitro activity of ciprofloxacin, a quinolone-carboxylic acid derivative, was compared with those of carbenicillin, azlocillin, cefsulodin, ceftazidime, tobramycin and amikacin against 187 non-fermenters. only one of the 131 strains of pseudomonas spp. was not inhibited by 1 mg/l of ciprofloxacin, while these isolates appeared highly resistant to carbenicillin, azlocillin and cefsulodin. ciprofloxacin was also the best agent against flavobacterium, alcaligenes faecalis and acinetobacter ca ... | 1985 | 3159710 |
| the in-vitro activities of enoxacin and ofloxacin compared with that of ciprofloxacin. | the in-vitro activities of enoxacin and ofloxacin were compared with that of the other new 4-quinolone, ciprofloxacin. all three compounds were highly active against enterobacteriaceae, haemophilus influenzae and neisseria gonorrhoeae (mics mostly less than 1 mg/l). the other gram-negative aerobes tested were in general less susceptible, though for acinetobacter and pseudomonas species (including aeruginosa) mics seldom exceeded 8 mg/l. ofloxacin and ciprofloxacin were more active against gardne ... | 1985 | 3159712 |
| ciprofloxacin disk susceptibility tests: interpretive zone size standards for 5-microgram disks. | evaluations of 5-microgram ciprofloxacin disk diffusion susceptibility tests were performed independently by seven different investigators. the results of the separate tests were combined to increase the number of resistant strains in the challenge set of microorganisms. based on data with 2,652 isolates, the following interpretive breakpoints are tentatively proposed for use in ongoing clinical trials of ciprofloxacin: less than or equal to 15 mm, resistant (mic greater than 2.0 micrograms/ml); ... | 1985 | 3159749 |
| ciprofloxacin for selective decontamination of the alimentary tract in patients with acute leukemia during remission induction treatment: the effect on fecal flora. | ciprofloxacin, a new quinolone derivative, was given prophylactically (500 mg twice a day) to 15 patients with acute leukemia during remission induction treatment for a mean duration of 42 days. the effect on the microbial flora of the alimentary tract was evaluated. a rapid elimination of enterobacteriaceae within three to five days was observed. bacteroides and clostridium species were not affected, but the number of anerobic nonsporeforming gram-positive bacilli and anaerobic cocci was decrea ... | 1985 | 3159811 |
| [comparative in vitro study of the activity of 5 quinolones against acinetobacter calcoaceticus]. | the authors compared the in vitro activity of ofloxacin, pefloxacin, ciprofloxacin, norfloxacin and nalidixic acid against 143 clinical isolates of acinetobacter calcoaceticus. minimal inhibitory concentrations (mics) were determined by the agar dilution method. minimal bactericidal concentrations (mbcs) were measured using microplates. ciprofloxacin, ofloxacin and pefloxacin were substantially active in vitro. mics 50 were respectively 0.72, 0.69 and 1.05 micrograms/ml. geometric means were 0.9 ... | 1985 | 3162142 |
| [frequency of in vitro sensitivity to netilmicin and ceftizoxime on selected highly resistant flora in surgical intensive therapy]. | the in vitro sensitivity of 132 gram positive and gram negative bacterial strains to netilmycin and ceftizoxime was assessed in order to update the statistics on the a priori efficacy of the antibacterial drugs. the flora studied were carefully selected as an extreme case of resistance to the standard antibiotics. netilmycin was found to be effective against staphylococci, pseudomonas and enterobacter, ceftizoxime against serratia, acinetobacter, proteus and klebsiella. both drugs were equally e ... | 1988 | 3162558 |
| [susceptibilities of clinical isolates to antibacterial agents. focusing mainly on ofloxacin (first report). reported by the research group for testing ofloxacin susceptibility of clinical isolates]. | susceptibility tests were carried out on a variety of clinically isolated pathogens using the susceptibility disc method at 197 hospitals in japan between may, 1985 through march, 1986. these tests were organized by the research group for testing ofloxacin susceptibility on clinical isolates, and the results were statistically analyzed. this paper describes a comparison of susceptibilities of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, st ... | 1988 | 3163383 |
| preparation and characterization of monoclonal antibodies to cephalexin-synthesizing enzyme from acetobacter turbidans. | eleven monoclonal antibodies against the cephalexin-synthesizing enzyme have been constructed and primarily characterized. these antibodies are all igg1 type, with medium affinity, and with no enzyme-inhibition effect. they will be utilized as immunoadsorbents to purify their corresponding antigen, the enzyme, in one step. | 1988 | 3169806 |
| dna sequence of the acinetobacter calcoaceticus catechol 1,2-dioxygenase i structural gene cata: evidence for evolutionary divergence of intradiol dioxygenases by acquisition of dna sequence repetitions. | the dna sequence of a 1.6-kilobase-pair sali-kpni acinetobacter calcoaceticus restriction fragment carrying cata, the structural gene for catechol 1,2-dioxygenase i, was determined. the 933-nucleotide gene encodes a protein product with a deduced molecular weight of 34,351. the similarly sized pseudomonas clca gene encodes catechol 1,2-dioxygenase ii, an enzyme with relatively broad substrate specificity and relatively low catalytic efficiency. comparison of the cata and clca sequences demonstra ... | 1988 | 3170486 |
| [susceptibility of gram-negative bacterial isolates to six beta-lactam and two aminoglycoside antibiotics at our university hospital]. | susceptibilities of gram-negative bacterial strains isolated at the university hospital in 1987 to six beta-lactam antibiotics (azthreonam cefmetazole, ceftizoxime, latamoxef, aminobenzyl-penicillin and piperacillin) and two aminoglycoside antibiotics (gentamicin and amikacin) were examined by agar dilution and the agar diffusion method. results obtained from both methods correlated well in most of the strains. most of the strains belonging to escherichia coli, klebsiella oxytoca, klebsiella pne ... | 1988 | 3175388 |
| cytochrome b-562 from acinetobacter calcoaceticus l.m.d. 79.41. its characteristics and role as electron acceptor for quinoprotein glucose dehydrogenase. | a soluble cytochrome b was purified from acinetobacter calcoaceticus l.m.d. 79.41. on the basis of the alpha-band maximum of a reduced preparation, measured at 25 degrees c, it is designated as cytochrome b-562. this cytochrome is a basic monomeric protein (pi 10.2; mr 18,000), containing one protohaem group per molecule. the reduced form, at 25 degrees c, showed absorption bands at 428, 532 and 562 nm. at 77 k the alpha-band shifted to 560 nm (with a shoulder at 558 nm). the reduced cytochrome ... | 1988 | 3178744 |
| synergistic interactions of ciprofloxacin and extended spectrum beta-lactams or aminoglycosides against acinetobacter calcoaceticus ss. anitratus. | the susceptibility of 54 clinical isolates of acinetobacter calcoaceticus ss. anitratus to 16 antimicrobial agents was determined in vitro with inoculum of 10(4) and 10(6) cfu by a standard agar dilution method. the most active agents were imipenem, sch 34343, ciprofloxacin, difloxacin (a-56619), and a-56620. only imipenem and abbott quinolones (a-56619 and a-56620) remained active when tested with the heavier inoculum. except for ticarcillin and ceftazidime, which showed only moderate activity, ... | 1988 | 3180706 |