Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| [Epidemiology and mechanisms of pneumonia in adults]. | Pneumonia is a major cause of morbidity and mortality in France. Microbial epidemiology, profile of bacterial resistance and prognosis depend on the type of pneumonia (community acquired pneumonia [CAP], health-care associated pneumonia, hospital acquired pneumonia or ventilator associated bacterial pneumonia). Streptococcus pneumoniae, community respiratory viruses, Mycoplasma pneumoniae, Chlamydia pneumonia and Legionella sp. are the five most common pathogens causing CAP in adults. The suscep ... | 2011 | 22135968 |
| [when to ask a microbiological diagnostic test in pneumonia and how to interpret the results]. | pneumonia is a leading cause of death in adults in the industrialised countries and in the rest of the world in infants. most of the time the pathogens stay unkown and the current recommendations for the treatment of pneumonia are mainly based on a severity score to adapt the treatement to the risk. nonethless new pathogens have recently been identified, coronavirus for srass, h5n1 for avian flu, n1n1 for pandemic flu, 35 years after the identification of legionella pneumophila. these exemples s ... | 2011 | 22135970 |
| Identification of Anaplasma marginale Type IV Secretion System Effector Proteins. | Anaplasma marginale, an obligate intracellular alphaproteobacterium in the order Rickettsiales, is a tick-borne pathogen and the leading cause of anaplasmosis in cattle worldwide. Complete genome sequencing of A. marginale revealed that it has a type IV secretion system (T4SS). The T4SS is one of seven known types of secretion systems utilized by bacteria, with the type III and IV secretion systems particularly prevalent among pathogenic Gram-negative bacteria. The T4SS is predicted to play an i ... | 2011 | 22140462 |
| roles of il-17 in an experimental legionella pneumonia model. | interleukin (il)-17 is a key factor in t helper type (th) 17 lineage host responses and plays critical roles in immunological control of a variety of infectious diseases. although legionella pneumophila, an intracellular bacterium found widely in the environment, often causes a serious and life-threatening pneumonia in humans, the contribution of il-17 to immune function during legionella pneumonia is unknown. in the present study, we used an experimental legionella pneumonia infection to clarif ... | 2011 | 22144493 |
| prevalence of legionella spp. in water systems of hospitals and hotels in south western greece. | the aim of the present study was to determine the prevalence of legionella spp. in water systems of hospitals and hotels located in south western greece, to study the molecular epidemiology of the isolated strains and their possible association with bacterial contamination (total count and pseudomonas aeruginosa), the water ph, and temperature. a prevalence survey for legionella spp. by culturing techniques in water distribution systems of eight hospitals and nine hotels occurred in south wester ... | 2011 | 22149148 |
| empyema caused by legionella pneumophila. | 2011 | 22153580 | |
| Legionella pneumonia presenting with bilateral flank pain, hyponatraemia and acute renal failure. | Legionnaires' disease (LD) is an often overlooked but a possible cause of sporadic community acquired pneumonia. High fever, cough and gastrointestinal symptoms are non-specific symptoms. Hyponatremia is more common in LD than pneumonia linked with other causes. A definitive diagnosis is usually confirmed by culture, urinary antigen testing for Legionella species. Macolide or quinolone antibiotic is the treatment of choice. We describe a case of Legionella pneumonia presenting with high fever, b ... | 2011 | 22154178 |
| Evaluation of empirical antibiotherapy for acute community-acquired pneumonia prescribed in emergency departments. | OBJECTIVE: The authors assessed the management of community-acquired pneumonia (CAP) and empirical antibiotic prescription in a primary care center. METHOD: A retrospective study was made on medical charts of patients admitted to the emergency department for CAP. RESULTS: Seventy-six patients were included. Their mean age was 65years for a sex ratio of 1.81. Fever (83%) and cough (72%) were the most common symptoms. Chest X-ray was abnormal in 86%. The median CRP value was 138mg/L (58-235). The ... | 2011 | 22154524 |
| Legionella pneumophila regulates the small GTPase Rab1 activity by reversible phosphorylcholination. | Effectors delivered into host cells by the Legionella pneumophila Dot/Icm type IV transporter are essential for the biogenesis of the specialized vacuole that permits its intracellular growth. The biochemical function of most of these effectors is unknown, making it difficult to assign their roles in the establishment of successful infection. We found that several yeast genes involved in membrane trafficking, including the small GTPase Ypt1, strongly suppress the cytotoxicity of Lpg0695(AnkX), a ... | 2011 | 22158903 |
| the major facilitator superfamily-type protein lbtc promotes the utilization of the legiobactin siderophore by legionella pneumophila. | the gram-negative bacterium legionella pneumophila elaborates the siderophore legiobactin. we previously showed that cytoplasmic lbta helps mediate legiobactin synthesis, inner membrane lbtb promotes export of legiobactin, and outer membrane lbtu acts as the ferrisiderophore receptor. rt-pcr analyses now identified lbtc as an iron-repressed gene that is the final gene in an operon containing lbta and lbtb. in silico analysis predicted that lbtc is an inner membrane protein that belongs to the ... | 2011 | 22160401 |
| [Pathogenicity factors of bacteria with glycosylating activity]. | A and B toxins of Clostridium difficile, a-toxin of C. novyi, lehal toxin of C. sordellii, and TpeL toxin of C. perfringens belong to the group of the so-called large Clostridium toxins. These toxins modify low-molecular weight guanosine triphosphate-binding proteins of the Rho/Ras family by their glycosylation that results in inactivation of major signal pathways in eukaryotic cells. Lgt glycosyltransferases, a new group of pathogenicity factors also capable of inactivating eukaryotic substrate ... | 2011 | 22168036 |
| performance on a multipathogen external quality assessment (eqa) panel by different mono- and multiplex nucleic acid amplification tests. | an external quality assessment (eqa) panel consisting of a total of 48 samples in broncho alveolar lavage (bal) or transport medium was prepared in collaboration with quality control for molecular diagnostics (qcmd) (www.qcmd.org). the panel was used to assess the proficiency of the 3 laboratories that would be responsible to examine the 6000 samples to be collected in the grace network of excellence (www.grace-lrti.org). the main objective was to decide on the best performing testing approach f ... | 2011 | 22170925 |
| [Demographic characteristics of reported cases of legionellosis in years 2005-2009 in Poland in comparison to EWGLI data]. | In Poland, all forms of legionellosis are obligatory reported: Legionella pneumonia and extrapulmonary legionellosis (such as Pontiac fever). Total 210 cases caused by Legionella sp. were reported in years 2003-2010. In our study analysis of age, sex, place of residence of 163 patients with legionellosis reported in 2005-2009 were done. The majority (74.2%) cases were reported in Region Mazowieckie. Age and sex structure of patients in Poland in years 2005-2009 differ from the data of EWGLI. In ... | 2011 | 22184944 |
| [legionella contamination of hospital hot water supply systems in the light of research conducted in 2008-2010 as part of supervision by the country sanitary inspector in bydgoszcz]. | the aim of the study was to assess the level of legionella sp. contamination in the hot water supply systems at the premises of inpatient healthcare facilities. in the years 2008-2010 the state poviat sanitary inspector in bydgoszcz tested the hot water supply systems in 8 hospitals. a total of 88 samples of hot water were collected in the years 2008-2010. the analysis involved temperature measurements and microbiological testing of the hot water. contamination levels exceeding the applicable st ... | 2011 | 22184945 |
| Diagnostic Challenges and Opportunities in Older Adults With Infectious Diseases. | Infections remain a major threat to the well-being of our growing aged population. The correct and timely diagnosis of infections in older adults is increasingly important in the current age of antimicrobial resistance. Urinary tract infection, pneumonia, and bacteremia present particular challenges. In older patients with bacteremia, blood cultures have comparable yield as compared with younger patients. However, the routine triggers for ordering blood cultures may not be appropriate in older a ... | 2011 | 22186775 |
| The First Case of Legionella nagasakiensis Isolation from Hot Spring Water. | In August, 2010, strain HYMO-6 was isolated from a sample of hot spring water in Aomori, Japan. The 16S rDNA sequences (1,496bp) of this strain (accession number: AB597175) had a similarity of less than 96.6% to other Legionella species, prompting us to hypothesize that this strain might be a novel species belonging to the genus Legionella. However, in March of 2011, it was became clear that the HYMO-6 strain (=JCM 17450 =KCTC 23560 =DSM 24727) was Legionella nagasakiensis CDC-1796-JAP-E(T) (=AT ... | 2011 | 22190440 |
| Legionella pneumophila transcriptional response to chlorine treatment. | Legionella pneumophila is a ubiquitous environmental microorganism found in freshwater that can cause an acute form of pneumonia known as Legionnaires' disease. Despite widespread use of chlorine to ensure drinking water quality and awareness that L. pneumophila may escape these treatments, little is known about its effects on L. pneumophila. The aim of this study was to investigate the L. pneumophila transcriptional response induced by chlorine treatment. Transcriptome analysis, using DNA array ... | 2011 | 22192759 |
| Electrochemically amplified molecular beacon biosensor for ultrasensitive DNA sequence-specific detection of Legionella sp. | An electrochemically amplified molecular beacon (EAMB) biosensor is constructed using thiolated hairpin DNA-ferrocene probes on gold electrode. The switching from "on" to "off" states of individual probes in the presence of complementary DNA target influences the electrode potential, besides the current, owing to changes in surface density of the electroactive hairpin DNA-ferrocene probes. The EAMB biosensor demonstrates linear range over 8 orders of magnitude with ultrasensitive detection limit ... | 2011 | 22196877 |
| Legionella pneumophila community-acquired pneumonia (CAP) in a post-splenectomy patient with myelodysplastic syndrome (MDS). | Legionnaire's disease is a cause of community-acquired pneumonia (CAP) in normal hosts, but those with impaired cell-mediated immunity (CMI) and T-lymphocyte function are particularly predisposed to Legionella species CAP. Myelodysplastic syndrome (MDS) is a disorder of the elderly that is associated with impaired CMI. Cases of MDS or Legionella species CAP are rare. Splenectomized patients primarily have impaired humoral immunity and B-lymphocyte function, and, to a lesser extent, some decrease ... | 2011 | 22197305 |
| legionella species in year-round vs. seasonal accommodation water supply systems. | the purpose of this study was to compare the quality of hot water between eleven hotels in the split-dalmatia county, croatia that are open year round and 10 summer season hotels and retirement homes with irregular use of water. we took 122 samples between may and december 2009. water temperature and free residual chlorine were measured in situ. physical and chemical analysis included ph, electrical conductivity, and concentrations of iron, manganese, copper, zinc, calcium, and magnesium that we ... | 2011 | 22202467 |
| development of a miniaturized dna microarray for identification of 66 virulence genes of legionella pneumophila. | introduction: for the last five years, legionella sp. infections and legionnaire's disease in poland have been receiving a lot of attention, because of the new regulations concerning microbiological quality of drinking water. this was the inspiration to search for and develop a new assay to identify many virulence genes of legionella pneumophila to better understand their distribution in environmental and clinical strains. the method might be an invaluable help in infection risk assessment and i ... | 2011 | 22204761 |
| legionella pneumophila st1/paris-pulsotype subtyping by spoligotyping. | endemic strains of legionella pneumophila st1, in particular the st1/paris-pulsotype, are dispersed worldwide and represent about 10% of culture-proven clinical cases of ld in france. the high isolation rate of this strain, in both clinical and environmental samples, makes identification of the source of infection difficult during epidemiological investigations. the full-length genome sequence of this strain was recently determined and it revealed the presence of a crispr/cas complex. the aim of ... | 2011 | 22205819 |
| legionellae in engineered systems and use of quantitative microbial risk assessment to predict exposure. | while it is well-established that legionella are able to colonize engineered water systems, the number of interacting factors contributing to their occurrence, proliferation, and persistence are unclear. this review summarizes current methods used to detect and quantify legionellae as well as the current knowledge of engineered water system characteristics that both favour and promote legionellae growth. furthermore, the use of quantitative microbial risk assessment (qmra) models to predict pote ... | 2011 | 22209280 |
| inhibition of bacterial carbonic anhydrases and zinc proteases: from orphantargets to innovative new antibiotic drugs. | zinc-containing enzymes, such as carbonic anhydrases (cas) and metalloproteases (mps) play critical functions in bacteria, being involved in various steps of their life cycle, which are important for survival, colonization, acquisition of nutrients for growth and proliferation, facilitation of dissemination, invasion and pathogenicity. the development of resistance to many classes of clinically used antibiotics emphasizes the need of new antibacterial drug targets to be explored. there is a weal ... | 2012 | 22214451 |
| eight years of legionnaires' disease transmission in travellers to a condominium complex in las vegas, nevada. | summarytravel is a risk factor for legionnaires' disease. in 2008, two cases were reported in condominium guests where we investigated a 2001 outbreak. we reinvestigated to identify additional cases and determine whether ongoing transmission resulted from persistent colonization of potable water. exposures were assessed by matched case-control analyses (2001) and case-series interviews (2008). we sampled potable water and other water sources. isolates were compared using sequence-based typing. f ... | 2012 | 22214820 |
| aminoacyl-trna-charged eukaryotic elongation factor 1a is the bona fide substrate for legionella pneumophila effector glucosyltransferases. | legionella pneumophila, which is the causative organism of legionnaireś disease, translocates numerous effector proteins into the host cell cytosol by a type iv secretion system during infection. among the most potent effector proteins of legionella are glucosyltransferases (lgt's), which selectively modify eukaryotic elongation factor (eef) 1a at ser-53 in the gtp binding domain. glucosylation results in inhibition of protein synthesis. here we show that in vitro glucosylation of yeast and mous ... | 2011 | 22216304 |
| co-existence of legionella and other gram-negative bacteria in potable water from various rural and urban sources. | 2011 | 22216808 | |
| an outbreak of legionnaires disease associated with a decorative water wall fountain in a hospital. | objective. to detect an outbreak-related source of legionella, control the outbreak, and prevent additional legionella infections from occurring. design and setting. epidemiologic investigation of an acute outbreak of hospital-associated legionnaires disease among outpatients and visitors to a wisconsin hospital. patients. patients with laboratory-confirmed legionnaires disease who resided in southeastern wisconsin and had illness onsets during february and march 2010. methods. patients with ... | 2012 | 22227989 |
| legionella pneumophila lida affects nucleotide binding and activity of the host gtpase rab1. | legionella pneumophila, the causative agent of a severe pneumonia known as legionnaires' disease, intercepts material from host cell membrane transport pathways to create a specialized vacuolar compartment that supports bacterial replication. delivery of bacterial effector proteins into the host cell requires the dot/icm type iv secretion system. several effectors, including sidm, sidd, and lepb, were shown to target the early secretory pathway by manipulating the activity of the host guanosine ... | 2012 | 22228731 |
| legionella micdadei prosthetic valve endocarditis complicated by brain abscess: case report and review of the literature. | legionella endocarditis is extremely uncommon, and embolic phenomena have never been reported. we report the first case of legionella micdadei prosthetic valve endocarditis complicated by brain abscess. a 57-y-old immunocompromised woman with a history of mitral valve replacement developed confusion and left-sided weakness. brain magnetic resonance imaging showed a 3-cm peripheral-enhancing mass. transoesophageal echocardiography suggested a perivalvular abscess. blood cultures and valve culture ... | 2012 | 22235842 |
| legionella pneumophila secretes a mitochondrial carrier protein during infection. | the mitochondrial carrier family (mcf) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. mcf proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. we discovered a novel mcf member, termed legionellanucleotide carrier protein (lncp), encoded in the genome of legionella pneumophila, the causative agent of legionnaire's disease. lncp was se ... | 2012 | 22241989 |
| innate immune pathways triggered by listeria monocytogenes and their role in the induction of cell-mediated immunity. | acquired cell-mediated immunity to listeria monocytogenes is induced by infection with live, replicating bacteria that grow in the host cell cytosol, whereas killed bacteria, or those trapped in a phagosome, fail to induce protective immunity. in this chapter, we focus on how l. monocytogenes is sensed by the innate immune system, with the presumption that innate immunity affects the development of acquired immunity. infection by l. monocytogenes induces three innate immune pathways: an myd88-de ... | 2012 | 22244582 |
| [hygienic assessment of the characteristics of centralized hot water supply in case of moscow]. | the paper considers the characteristics of domestic hot water consumption by moscow dwellers. it gives the results of questionnaire surveys in 100 respondents aged 20-80 years about the modes and intensity of hot water use, by indicating the time and frequency of contact with hot water complaints about hot water supply. according to the authors'studies, the daily consumption of hot water per capita was 1.1-2 times greater than that of cold water; at the same time the total water use remained to ... | 2011 | 22250387 |
| live legionella pneumophila induces muc5ac production by airway epithelial cells independently of intracellular invasion. | the airway epithelium is the initial barrier against airborne pathogens, and it plays many roles in host airway defense. legionella pneumophila is an intracellular pathogen that causes rapidly advancing pneumonia and is sometimes life-threatening. here, we evaluated the role of the airway epithelial cells in the defense against l. pneumophila by examining mucus production in vitro. the production of muc5ac, a major mucin protein, was not induced by formalin- or ultraviolet-killed l. pneumophil ... | 2012 | 22260247 |
| legionella longbeachae and endocarditis. | we report a case of infectious endocarditis attributable to legionella longbeachae. l. longbeachae is usually associated with lung infections. it is commonly found in composted waste wood products. l. longbeachae should be regarded as an agent of infectious endocarditis, notably in the context of gardening involving handling of potting soils. | 2012 | 22261182 |
| domain structure and denaturation of a dimeric mip-like peptidyl prolyl cis-trans isomerase from escherichia coli. | fkbp22, a protein expressed by escherichia coli, possesses ppiase (peptidyl-prolyl cis-trans isomerase) activity, binds fk506 (an immunosuppressive drug) and shares homology with legionella mip (a virulence factor) and the related proteins. to understand the domain structure and the folding / unfolding mechanism of mip-like proteins, a recombinant e. coli fkbp22 (his-fkbp22) was investigated here as a model protein. limited proteolysis indicated that his-fkbp22 harbors an n-terminal domain (ntd) ... | 2012 | 22263615 |