Publications

non-specific biosynthesis of hopane triterpenes by a cell-free system from acetobacter pasteurianum.	1. a cell-free system from the bacterium acetobacter pasteurianum was incubated with [12-3h]squalene; diploptene and diplopterol, hopanoids normally present in the bacterium, were labelled. their radioactivity was confirmed by purification using thin-layer chromatography, synthesis of derivatives and recrystallization to constant specific activity. this demonstrates the direct cyclization of squalene into diploptene and diplopterol, catalysed by a squalene cyclase activity in a. pasteurianum. 2. ...	1980	7460938
[bacterium mb 58, a methylotrophic "acetic acid bacterium"].		1980	7222745
cellulose biogenesis: polymerization and crystallization are coupled processes in acetobacter xylinum.	calcofluor white st, stilbene derivative used commerically as an optical brightener for cellulose, increased the rate of glucose polymerization into cellulose by resting cells of the gram-negative bacterium acetobacter xylinum. this bacterium normally produces a ribbon of cellulose that is a composite of crystalline microfibrils. in concentrations above 0.1 mm, calcofluor disrupts the assembly of crystalline cellulose i microfibrils and their integration into a composite ribbon by stoichiometric ...	1980	16592918
conversion of glycerol to dihydroxyacetone by immobilized whole cells of acetobacter xylinum.	enzymatic production of dihydroxyacetone (dha) was studied by immobilization of the whole cells of acetic acid bacteria capable of oxidizing glycerol to dha. acetobacter xylinum a-9 cells immobilized in a polyacrylamide gel were selected as the most favorable enzyme preparation. the enzymatic properties of immobilized cells converting glycerol to dha were investigated and compared with those of intact cells. the optimum ph for the immobilized cells was broad (4.0 to 5.5), whereas the intact cell ...	1979	16345471
[candida mycoderma growth inhibition with phenol and the autoselection of resistent forms under continuous ph-stat cultivation].	the effect of phenol on the growth rate and respiration was studied with the yeast candida mycoderma cultivated in the ph-static conditions with continuous recording of the principal kinetic parameters of the population. the kinetics of growth inhibition with phenol was studied. adaptation of the culture in terms of the growth rate and the rate of oxygen uptake was detected within 10--15 hours of cultivation. a new strain of c. mycoderma phen. r. isolated using the technique of autoselection upo ...	1979	39228
distribution of coenzyme f420 and properties of its hydrolytic fragments.	the ability of hydrolytic products of coenzyme f420 to substitute for f420 in the hydrogenase and nicotinamide adenine dinucleotide phosphate-liniked hydrogenase systems of methanobacterium strain m.o.h. was kinetically determined. the nicotinamide adenine dinucleotide phosphate-linked hydrogenase system was employed to quantitate the levels of f420 in a number of methanogenic bacteria as well as in some nonmethanogens. methanobacterium ruminantium and methanosarcina barkeri contained low levels ...	1979	40952
the interaction of alpha-chlorohydrin with glycerol kinase.	alpha-chlorohydrin has been examined both for its ability to act as a substrate for glycerol kinase and as an inhibitor of the reaction of glycerol with glycerol kinase. using a purified enzyme from candida mycoderma, it was established that alpha-chlorohydrin does not act as a substrate for glycerol kinase, but does act as a competitive inhibitor (ki of 30 mm) of purified glycerol kinase and the enzyme present in a sonicated preparation of ram spermatozoa. neither alpha-chlorohydrin nor alpha-c ...	1979	225494
[functional characteristics of the electron transport chain in candida mycoderma yeasts exposed to benzhydroxamic acid].	the effect of antimycin a and benzhydroxamic acid (bha) on functioning of the electron transport system was studied with the resting cells of candida mycoderma grown in a medium containing glucose and collected at the beginning of the deceleration phase. in the original ("control") cells, the processes of oxygen consumption were shown to be mediated mainly by the phosphorylating electron transport chain. when the cells were incubated withe glucose, the cyanide resistant electron transport chain ...	1979	423810
visualization of pores (export sites) correlated with cellulose production in the envelope of the gram-negative bacterium acetobacter xylinum.	the gram-negative bacterium acetobacter xylinum assembles a cellulse ribbon composed of a number of microfibrils in the longitudinal axis of its envelope. the zone of ribbon assembly was investigated by freeze-etch electron microscopy. freeze-etching revealed, beneath the cellulose ribbons, a linear array of pores on the lipopolysaccharide membrane. these pores have a rim diameter of 120--150 a and a central hole or deepening of approximately 35 a. the axes of pore arrays closely coincide with l ...	1979	457769
complete degradation of carbohydrate to carbon dioxide and methane by syntrophic cultures of acetobacterium woodii and methanosarcina barkeri.	methanosarcina barkeri (strain ms) grew and converted acetate to co2 and methane after an adaption period of 20 days. growth and metabolism were rapid with gas production being comparable to that of cells grown on h2 and co2. after an intermediary growth cycle under a h2 and co2 atmosphere acetate-adapted cells were capable of growth on acetate with formation of methane and co2. when acetate-adapted methanosarcina barkeri was co-cultured with acetobacterium woodii on fructose or glucose as subst ...	1979	464732
[efficiency of glucose utilization by gluconobacter oxydans].	the dynamics of growth and acid production in gluconobacter oxydans cultures at various glucose concentrations has been investigated. dinitrophenol (10-4 m) was shown to have effect on hexonic acids formation by the growing culture and resting cells of g. oxydans, as well as on the values of y0. g. oxydans molar growth yield for glucose have been calculated. oxidative transformation of glucose was shown to be not involved in energy supply of processes connected with the reproduction of g. oxydan ...	1979	470626
[systematics of acetic acid bacteria].	the method of numerical taxonomy has been used in these studies. fifty-six strains of acetic acid bacteria are characterized in 136 phenotypical features. the coefficients of similarity of the strains were calculated using computer. the nucleotide composition of dna from 21 strains of acetic acid bacteria was determined. the results obtained by the method of numerical taxonomy were discussed basing on this genotypical criterion. the genera acetobacter beijerinck and gluconobacter asai were found ...	1979	470639
beta-1,2-glucosyl transfer by membrane preparations from acetobacter xylinum.		1979	499548
additional properties of a soluble polymer of glucose from cultures of acetobacter xylinum.	the results of differential, thermal analysis of a soluble, beta (1 leads to 2)-branched, beta (1 leads to 4)-d-glucan isolated from cultures of acetobacter xylinum are consistent with previous conclusions about its structure. the o-acetyl content of the polymer is 8.3% which corresponds to a maximum substitution of one acetyl group per three glucose residues. proton nuclear magnetic resonance spectra confirm that all the glycosidic bonds are beta linkages. some preparations of the polymer are c ...	1979	540240
[effect of yeast growth limitation on the respiratory chain, the economic coefficient and the critical oxygen concentration for respiration].	the effect of the yeast growth limitation by oxygen on the economical coefficient (ec), the operation of the cyanide resistant electron transport pathway (cretp), and the critical for respiration oxygen concentration concentration ([o2]cr) was studied. the operation of cretp was found to differ among various yeasts growing on glucose: it could function during both the exponential phase and limitation of growth (torulopsis candida), or only in the conditions of growth limitation (candida tropical ...	1978	568709
purification and regulatory properties of the oxaloacetate decarboxylase of acetobacter xylinum.	the oxaloacetate (oaa) decarboxylase (ec 4.1.1.3) activity of acetobacter xylinum cells grown on glucose or glycerol is the same as that of cells grown on intermediates of the citrate cycle. the enzyme was purified 92-fold from extracts, and its molecular weight was determined to be 100,000 by gel filtration. initial velocity studies revealed marked positive cooperativity for oaa (hill coefficient [n(h)] = 1.8; s(0.5) = 21 mm). the affinity of the enzyme for oaa was markedly increased upon addit ...	1978	206534
bacterial 2,3-butanediol dehydrogenases.	enterobacter aerogenes, aeromonas hydrophila, serratia marcescens and staphylococcus aureus possessing l(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; acetobacter suboxydans, bacillus polymyxa and erwinia carotovora containing d(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. the d(-)-butanedio ...	1978	25056
a conjugate of cellulase with fluorescein isothiocyanate: a specific stain for cellulose.	a fluorescent technique has been developed for in situ staining of cellulose. the staining agent in conjugate of cellulase and fluorescein isothiocyanate (fitc). application of this agent does not disturb intercellular or intracellular substances. the technique depends on the specific binding of the fluorescent labeled enzyme to its substrate. the stain has been tested on cell-free noncellulose polysaccharides similar to cellulose and does not stain them. the technique has been used to localize ...	1978	80840
lipid and fatty acid composition of gluconobacter oxydans before and after intracytoplasmic membrane formation.	gluconobacter oxydans differentiates by forming quantities of intracytoplasmic membranes at the end of exponential growth, and this formation occurs concurrently with a 60% increase in cellular lipid. the present study was initiated to determine whether this newly synthesized lipid differed from that extracted before intracytoplasmic membrane synthesis. undifferentiated exponential-phase cells were found to contain 30% phosphatidylcholine, 27.1% caridolipin, 25% phosphatidylethanolamine, 12.5% p ...	1978	649571
intra- and intergeneric similarities of the rrna cistrons of acetobacter and gluconobacter [proceedings].		1978	655696
tetrahydrofolate enzyme levels in acetobacterium woodii and their implication in the synthesis of acetate from co2.	acetate synthesis from co2 by acetobacterium woodii may occur as in homoacetate-fermenting clostridia, as indicated by high levels of enzymes of the tetrahydrofolate pathway and by pyruvate-dependent formation of acetate from methyl-b12 and methyltetrahydrofolate.	1978	659361
regulatory properties of the alpha-ketoglutarate dehydrogenase complex of acetobacter xylinum. in situ studies and localization of the allosteric response in the e1 component.		1978	670220
morphology microstructure, and development of colonies of acetobacter xylinum.	development of the morphology and microstructure of colonies of acetobacter xylinum growing on agar was studied by optical microscopy, and transmission and scanning electron microscopy. the mass of rapidly dividing cells surrounded by a sheath of cellulose microfibrils passes from a smooth spheroid to a flattened aggregate with a characteristic "pillowed" surface. this morphology is the result of a repeated extrusion of cells from the confirming sheath, followed by regeneration of a new portion ...	1978	679065
[effect of cultivation conditions on the cytochrome system of candida mycoderma yeasts].	the effect of aeration of the medium, the source of carbon, yeast autolysate and its components (amino acids, vitamins, cytochrome precursors) on the biosynthesis of cytochromes and the ratio between them was studied in the cells of candida mycoderma. the content of cytochromes b and c increased in the cells at the stationary phase of growth on the rieder medium regardless of the carbon source and in the presence of elevated concentrations of iron and yeast autolysate (or one of its components, ...	1978	703641
aflatoxin production by aspergillus parasiticus in a competitive environment.	aspergillus parasiticus nrrl 2999 was grown in the presence of rhizopus nigricans, saccharomyces cerevisiae, acetobacter aceti, or brevibacterium linens and aflatoxin concentration was determined after 3,5,7, and 10 days of incubation at 28c. r. nigricans and s. cerevisiae inhibited growth and aflatoxin production by a. parasiticus. b. linens caused slight inhibition and a. aceti stimulated growth and aflatoxin production by a. parasiticus.	1977	339094
[evidence of the haptenic nature of peptidomannans from two yeasts: saccharomyces oviformis and candida mycoderma].	following the observation that it is not possible to immunize rabbits against peptidomannans, extracted from saccharomyces oviformis and candida mycoderma, these glycopeptides have been coupled to bovine serum albumine in order to make them immunogenic. two methods have been used for the coupling: the cyanogen bromide method and the carbodiimide method. rabbits have been injected intravenously with these conjugates. using different serologic technics: agglutination, immunodiffusion, counterimmun ...	1977	412102
kinetics of dithionite ion utilization and atp hydrolysis for reactions catalyzed by the nitrogenase complex from azotobacter vinelandii.	the kinetics of s2o42-utilization and atp hydrolysis during the nitrogenase-catalyzed h2 evolution and acetylene and nitrogen-reducing reactions were studied using a polarographic technique to monitor-s2o42-concentration. rate constants for both s2o42-utilization and atp hydrolysis were determined as a function of temperature and corresponding activation energies determined. the activation energy for atp hydrolysis differs from that for product formation or s2o42-utilization by 5 kcal/mol above ...	1977	836787
oxidation of a branched-chain alditol by acetobacter suboxydans: a stereospecific synthesis of l-dendroketose.	a synthesis of l-dendroketose (5) has been achieved by microbiological oxidation by acetobacter suboxydans of the branched-chain alditol 2-c-(hydroxy-methyl)-d-erythro-pentitol (4). treatment of the oxidation product with acetone, copper(ii) sulfate, and sulfuric acid afforded the two di-o-isopropylidene-l-dendro-ketose derivatives 6 and 7. assignment of configuration at the branching carbon atom (c-4) and at the anomeric center in 6 and 7 was made on the basis of the carbon-13 magnetic resonanc ...	1977	844055
oxidation of glycosides by acetobacter suboxydans: synthesis of methyl alpha- and beta-l-threo-pentopyranosid-4-uloses.		1977	861970
the biosynthesis of cellulose by acetobacter xylinum and acetobacter acetigenus.		1977	871970
[endotoxins: a neglected environmental factor].	the effects of bacterial endotoxins are well known form an experimental and clinical point of view. their presence in the environment in general and their possible role for the development of clinical symptoms at low exposure levels has been less studied. experimental and epidemiological evidence for such effects are reviewed with reference to particular environments where this exposure might be present.	1977	878658
synthesis of polyprenol-monophosphate- beta -galactose by acetobacter xylinum.	a particulate enzyme preparation from acetobacter xylinum synthesizes ficaprenol-monophosphate-beta-galactose from ficaprenol monophosphate (fmp) and udp-galactose in the presence of detergent. the product has the same properties as those previously reported for the compound formed with the endogenous acceptor. dolichol-monophosphate (dolmp) is also a good galactose acceptor but the product obtained has different properties. lipid extracts from acetobacter contain galactose acceptor capacity whi ...	1977	887092
5-deoxy-5-fluoro-l-sorbose originating from 2-deoxy-2-fluoro-d-glucitol by fermentation with acetomonas oxydans.	using fermentation with a selected strain of acetomonas oxydans it was possible to convert 2-deoxy-2-fluro-d-glucitol to 5-deoxy-5-fluoro-l-sorbose, in agreement with bertrand's and hudson's rule. the last-named compound was isolated in a yield of 88%. both compounds were little toxic against acetomonas oxydans.	1977	892670
glucose metabolism in acetobacter aceti.	acetobacter aceti ncib 8554 grows on a minimal medium with ethanol but not with glucose as carbon and energy source. addition of glucose to a wild type culture on ethanol has no influence on growth of the organism. growth of a glucose sensitive mutant a5 is inhibited by the addition of glucose until all glucose has disappeared from the medium. in order to determine the routes by which glucose is metabolised in wild type and mutant, radiorespirometric, enzymatic, and uptake experiments have been ...	1977	907428
purification and properties of a soluble polymer of glucose from cultures of acetobacter xylinum.	a soluble nondialyzable polymer of glucose was isolated and purified by selective ethanol and ammonium sulfate precipitation from the supernatant of a culture of acetobacter xylinum which was actively producing cellulose. this polymer was heterogeneous in size with an average sedimentation constant s20,w, of the most abundant fraction of 11.1. on drying from dilute solution in water, the polymer(s) showed extended linear fibrils or aggregates of such fibrils by transmission electron microscopy. ...	1977	912597
formation of an acid-labile maltosyl-lipid by enzyme preparations from acetobacter xylinum.		1977	923800
[the role of polyprenols in sugar moiety transfer (author's transl)].		1977	67595
fermentation of glucose by acetobacter melanogenus.	growing cultures of acetobacter melanogenus atcc 9937 concerted d-glucose to 2,5-diketo-d-gluconic acid with d-gluconic acid and 5-keto-d-gluconic acid as intermediates. the 2,5-diketo-d-gluconic acid was isolated from the fermented medium by treatment with an anion exchange resin.	1977	15673
alpha-ketoglutarate dehydrogenase complex of acetobacter xylinum. purification and regulatory properties.	the alpha-ketoglutarate dehydrogenase complex of acetobacter xylinum was purified to homogeneity. it consists of three main polypeptide chains with a total molecular weight of about 2.4 x 10(6). it catalyzes the overall mg2+ and thiamin pyrophosphate-dependent, nad+- and coa-linked oxidative decarboxylation of alpha-ketoglutarate, as well as the partial reactions characteristic of the three enzyme components described for the complex from other sources. initial velocity studies revealed marked p ...	1977	16009
researches to the conversion of sorbit into sorbose by acetobacter suboxydans (author's transl).	the production of sorbose by acetobacter suboxydans (4) is closely related to the concentration of sorbit in the medium. an increasing concentration of sorbit gives rise to the inhibition of cell reproduction; followed by a decrease of sorbose content in the culture medium. the decrease of sorbose yield in concentrations of about 15% sorbit in medium indicates the decreasing metabolism rate of the total population of acetobacter suboxydans (4) culture and does not refer to the ability of the ind ...	1977	22208
[several physiologic characteristics of candida mycoderma yeasts and their mutant lacking cytochromes b and a+a3].		1977	190281
the structure of cellulose-producing bacteria, acetobacter xylinum and acetobacter acetigenus.	the structure of the pellicles and cells of the cellulose-producing bacteria, acetobacter xylinum and acetobacter acetigenus, was studied by transmission electron microscopy of thin sections and freeze-etch replicas of glucose-stimulated cell suspensions, quiescent cell suspensions, and discrete pellicles. these bacteria have a relatively thin cell wall in section, with several irregular features superimposed on an otherwise simple, gram-negative morphology. there are no flagella or pili. unfixe ...	1977	194664
[respiratory chain of candida mycoderma].	the respiration chain of the yeast candida mycoderma was studied during its growth on glucose. electrons can be transported from the pyridine nucleotide (pn) pool to oxygen by two pathways, as was shown in experiments with intact cells on the basis of inhibitory analysis of respiration and the extent of reduction of electron carriers. the first pathway is inhibited by cyanide and antimycin a; it includes pn, flavoprotein, (fp) and cytochromes b, c and a + a3. the second pathway of electron trans ...	1977	198640
[mutagenic treatment of acetobacter suboxydans with athylmethansulfonate (ems) (author's transl)].		1977	201127
[effect of oxygen concentration and the physiological state of cells on the respiration chain of candida mycoderma].	the effect of oxygen concentration and limitation of the cultural growth by the substrate to be oxidized on the functionation of the respiration chain and the ratio of cytochromes a : c : b was studied in intact cells of candida mycoderma. as the concentration of oxygen in the medium decreases and the growth of the culture slows down, the cyanide-resistant pathway of electron transport (crpet), which is inhibited by benzhydroxamic acid, is found in the yeast cells. at the same time, the content ...	1977	202840
electron microscopic investigation of the hydrogen-oxidizing acetate-forming anaerobic bacterium acetobacterium woodii.	acetobacterium woodii is a gram-positive anaerobic nonsporeforming bacterium able to grow on h2 and co2 as sole sources of energy. the product of fermentation is acetic acid. fine structural analysis showed rod-shaped flagellated cells, and coccoid cells without flagella arranged predominantly in pairs and chains. the cell wall was found to be composed of three layers. the cell surface exhibited a periodic array of particles consisting of subunits. the cytoplasmic membrane showed particles eithe ...	1977	596994
isolation of alpha-glucan and lipopolysaccharide fractions from acetobacter xylinum.	a cellular phenol-water extract of acetobacter xylinum nrc 17007 was fractionated on sepharose 4 b. the fraction eluting with the void volume consisted to about 95% of glycogen-like material. the lipopolysaccharide fraction was of lower molecular weight and had the following composition (%, w/w): mannose, 42; glucose, 7; galactose, 3.8; heptose, 2; 2-keto-3-deoxy-octonate, 1.2; glucosamine, 3.3; phosphate, 4.5; total fatty acids, 3.9. among the fatty acids, 3-hydroxy-tetradecanoic acid was prese ...	1977	603342
preparation and activity of immobilized acetobacter suboxydans cells.	the possibility of immobilization of acetobacter suboxydans cells by entrapment within polyacrylamide gels or by intercellular cross-linking with glutaral-dehyde was investigated. the oxidation of unsubstituted alditols and aldose diethyl dithioacetals by the resultant modified cells has been achieved.	1977	16345235
polyprenol kinase in acetobacter xylinum.		1976	210629
factors affecting the activity of citrate synthase of acetobacter xylinum and its possible regulatory role.	the citrate synthase activity of acetobacter xylinum cells grown on glucose was the same as of cells grown on intermediates of the tricarboxylic acid cycle. the activity of citrate synthase in extracts is compatible with the overall rate of acetate oxidation in vivo. the enzyme was purified 47-fold from sonic extracts and its molecular weight was determined to be 280000 by gel filtration. it has an optimum activity at ph 8.4. reaction rates with the purified enzyme were hyperbolic functions of b ...	1976	6002
characterization of the acetyl-coa synthetase of acetobacter aceti.	the acetate activating system of acetobacter aceti has been studied. the enzyme responsible, acetyl-coa synthetase, has been purified about 500-fold from crude cell extracts and was approximately 85% pure as judged by polyacrylamide gel electrophoresis in sodium dodecyl sulphate. the purified enzyme showed optimal activity at ph 7.6 in both tris-hcl and potassium phosphate buffers. in its purest form, the enzyme was stable at 4 degrees-c but denatured upon freezing. the km values for coa, atp an ...	1976	12800
method of determination of the activity of glycerol dehydrogenase in the submerged culture of acetobacter suboxydans.	the paper presents a method for assaying activity of glycerol dehydrogenase activity in the submerged culture of acetobacter suboxydans. the effect of substrate concentration, content of the submerged culture in the reaction mixture and ph value on the rate of enzymic oxidation of glycerol to dihygrooxyacetone by a. suboxydans has been studied.	1976	17111
desugarization of egg white by microorganisms.	glucose was eliminated from egg whites, using microorganisms, to prevent melanoidin formation which may damage the product. desugarization was achieved by means of acetobacter xylinum, streptococcus lactis, propionibacterium shermanii, pr. petersonii and propionicacid cocci. optimal conditions of desugarization were found, depending on the physiological characteristics of the above microorganisms. propionibacterium shermanii may be well used to ferment a liquid egg white. these bacteria have no ...	1976	17113
[electron transport chain of the candida mycoderma mutant lacking cytochromes b and a+a3].	two electrontransport chains--"oxygen" and "peroxidase"--were found to operate in the mutant of candida mycoderma lacking cytochromes b and a+a3; the terminal acceptors of electrons are oxygen and hydrogen peroxide, respectively. the "oxygen" chain lacks cytochromes but contains pyridine nucleotides and flavoproteins; it is inhibited by rotenone and benzhydroxamic acid. the "peroxidase" chain consists of pyridine nucleotides, cytochrome c, and cytochrome c peroxidase; it is inhibited by cyanide.	1976	187902
[raffinose metabolism in gluconobacter oxydans].	metabolism of raffinose has been examined in experiments with the growing culture and washed cells of gluconobacter oxydans l-1. degradtion of the trisaccharide was found to be catalyzed by levansucrase, levan being synthesized, and melibiose and small quantitites of fructose being liberated in the reaction. melibiose is not hydrolyzed and is not used by the bacterium as a source of carbon, but is oxidized to melibionic acid. fructose is assimilated by the bacterium in constructive metabolism, b ...	1976	933868
[a study of the cytochrome composition of microorganisms by their absorption spectra and first derivative spectra].	the cytochrome composition of candida mycoderma and torulopsis candida was studied by means of absorption spectra and spectra of the first derivative which were recorded at the temperature of liquid nitrogen and room temperature. the spectrum of the first derivative provided more information concerning the cytochrome composition than absorption spectra; data obtained with the aid of this spectrum at room temperature corresponded to those provided by means of absorption spectra at low temperature ...	1976	945440
phosphorylation of glycerol and dihydroxyacetone in acetobacter xylinum and its possible regulatory role.	extracts of acetobacter xylinum catalyze the phosphorylation of glycerol and dihydroxyacetone (dha) by adenosine 5'-triphosphate (atp) to form, respectively, l-alpha-glycerophosphate and dha phosphate. the ability to promote phosphorylation of glycerol and dha was higher in glycerol-grown cells than in glucose- or succinate-grown cells. the activity of glycerol kinase in extracts is compatible with the overall rate of glycerol oxidation in vivo. the glycerol-dha kinase has been purified 210-fold ...	1976	956117
cellulose saccharification for fermentation industry. applications.		1976	1000071
cellulose biosynthesis in acetobacter xylinum: visualization of the site of synthesis and direct measurement of the in vivo process.	in vivo synthesis of cellulose by acetobacter xylinum was monitored by darkfield light microscopy. cellulose is synthesized in the form of a ribbon projecting from the pole of the bacterial rod. the ribbon elongates at a rate of 2 mum min-1. the ribbon consists of approximately 46 microfibrils which average 1.6 x 5.8 nm in cross section. the observed microfibrillar elongation rate corresponds to 470 amol of glucose/cell per hr assimilated into cellulose. electron microscopy of the process using ...	1976	1070005
letter: nonspecific biosynthesis of hopane triterpenes in a cell-free system from acetobacter rancens.		1976	1249358
activities of citrate synthase and other enzymes of acetobacter xylinum in situ and in vitro.	the activities of a number of enzymes, extracted from acetobacter xylinum, that are involved in carbohydrate metabolism may be accounted for in situ in permeabilized cells. the kinetic properties of citrate synthase and glycerokinase observed in vitro are also retained in situ. so is the regulatory sensitivity of these enzymes. both in vitro and in situ, (a) citrate synthase, in contrast with the enzyme for other gram-negative bacteria, is inhibited by atp and is insensitive to nadh, and (b) gly ...	1976	1275900
inhibition by acetylene of conventional hydrogenase in nitrogen-fixing bacteria.		1976	778640
[a new aid in the diagnosis of fungi: microstix - candida test strips (author's transl)].	microstix test strips are a new aid in the diagnosis of yeasts which are easy to handle and can be used universally. observations on clinical material (a total of over 1200 test strips inoculated) involving 777 inoculated microstix with material from 6 different nursing areas show that altogether 36% postive findings, only 5.6% unspecifically postive (problem organisms and molds) and only 1.5% negative results were demonstrable with positive cultures of fungi on the control media. positive resul ...	1976	820970
cellulose synthesis by acetobacter xylinum. i. low molecular weight compounds present in the region of synthesis.	an analysis has been made of the low molecular weight fraction present in the region of cellulose synthesis in acetobacter xylinum suspensions. a number of nucleic acid bases, nucleosides and nucleotides, together with alpha-glucose 1-phosphate and udpg, were detected in various extracts of washed cells supplied with glucose. since glucose-6-p could be detected in extracts of ultrasonically disrupted cells, but not in extracts of whole cells, it was concluded that separate pools of hexose phosph ...	1975	803380
cellulose synthesis by acetobacter xylinum. ii. investigation into the relation between cellulose synthesis and cell envelope components.	cell envelope fractions, capable of cellulose synthesis from uridine diphosphate glucose, alpha-glucose-1-phosphate, glucose-6-phosphate and glucose, have been isolated from acetobacter xylinum suspensions and various enzymatic properties examined. essential enzymes were found to be distributed throughout the cell envelope region, with both inner (cytoplasmic) and outer (cell wall) membranes contributing to cellulose synthesis. the central role of udpg in cellulose synthesis was confirmed and th ...	1975	803381
biological value of the biomass and protein isolates from different yeasts.	the microbiological analysis with the test-organism tetrahymena pyriformis w can be used successfully to measure the relative nutritive value of the biomass and protein isolates from different yeasts for screening the groups of micro-organisms to choose those of the desired quality. in general, this method is rapid, comparatively cheap and gives the possibility to test large numbers of samples simultaneously. according to the literature and our own results, the relative nutritive value, the perc ...	1975	818566
effect of chlorpromazine on the respiration and hexose monophosphate dehydrogenases of gram-negative bacteria.	the drug chlorpromazine inhibits respiration in several gram-negative bacteria. the enzymes glucose-6-phosphogluconate dehydrogenase and 6-phosphogluconate dehydrogenase from these microorganisms are not affected by the drug.	1975	1090352
cellulose synthesis by acetobacter xylinum. iii. matrix, primer and lipid requirements and heat stability of the cellulose-forming enzymes.	the addition of soluble cellodextrins of increasing size to a cell envelope preparation of acetobacter xylinum stimulated cellulose synthesis from udpg. this stimulation was attributed to both acceptor and activator effects. enzymes required for cellulose synthesis were found to be heat-unstable and those required for synthesis of glycosylated lipid components from udpg, heat-stable. both heat-inactivated envelope fragments and supernatant fluid from whole cells were necessary for cellulose synt ...	1975	1111578
new evidence for an intermediate polymer of glucose in cellulose biosynthesis by acetobacter xylinum.	the results of sucrose-density-gradient centrifugation of a cell-free particulate enzyme system from acetobacter xylinum which was incubated with uridine diphosphoglucose indicate that there is a polymeric intermediate in the biosynthesis of cellulose. this intermediate has the properties of an oligomer of glucose, is normally attached to the heaviest particle of the suspension, but, when released by hydrolysis, is preferentially adsorbed to fragments of preformed cellulose. it may form short se ...	1975	1111860
further characterization of l-leucine-pyruvate transaminase from acetobacter suboxydans.	l-leucine-pyruvate transaminase obtained from acetobacter suboxydans exhibited absorbance maxima to 280 and 332 nm. the 332 nm peak was derived from the coenzyme bound to the enzyme protein with the epsilon nh2 of a lysine residue. the transaminase showed reactivity against many l-amino acids. the relation between the reactivity and the structure of the amino donor is discussed. the michaelis constants for l-leucine, pyruvate, l-alanine and alpha-ketoisocaproate were 6.7, 3.1, 7.1 and 0.9 mm, re ...	1975	1156585
nascent stage of cellulose biosynthesis.	freeze-etching of never-dried pellicles or of incubated suspensions of both acetobacter xylinum and acetobacter acetigenum show a nascent form of the cellulose microfibril which has a core surrounded by an amorphous sheath. drying of the pellicle or suspension reduces the diameter of the sheath and changes the form of the microfibril to the one usually seen. this nascent form of the cellulose microfibril is consistent with previous postulations of an intermediate polymer or polymers in the biosy ...	1975	1162359
[cultivation of the yeast mycoderma vini on an ethanol-containing medium and evaluation of the nutritive value of isolated yeast proteins].	a stable process of the development of the yeast mycoderma vini is possible during continuous cultivation on the nutrient medium containing 3% ethanol as the only carbon and energy source. the maximum coefficient of ethanol consumption is 65%. the total protein isolated from the yeast biomass is similar to casein in terms of the composition and susceptibility to gastrointestinal enzymes in vitro. this protein shows a low content of nucleic acids.	1975	1168903
proceedings: mechanism of action and regulatory behavior of alpha-ketoglutarate dehydrogenase from acetobacter xylinum.		1975	1205750
proceedings: phosphorylated forms of pyruvate, phosphate dikinase from acetobacter xylinum.		1975	1205752
[biosynthesis of bacterial cellulose from glucose selectively deuterated in position 6: nmr study].	d-glucose specifically deuterated at c-6 was prepared and used for the biosynthesis of bacterial cellulose with acetobacter xylinum. the material obtained was converted into glucitol hexaacetate and analyzed by 250 mhz n.m.r. and mass spectrometry. these spectra indicated that about 70% of the starting d-glucose was incorporated without modification of deuteriation at the c-6 position. however an explanation is required of the finding that deuterium was also incorporated at the c-2 and c-1 posit ...	1975	1227972
regulation of aspartokinase and homoserine dehydrogenase in acetic acid bacteria.	the regulation of aspartokinase and homoserine dehydrogenase has been studied in three acetobacter and two gluconobacter species. both enzymes were regulated by feedback inhibition. aspartokinase was inhibited by l-threonine and concertedly inhibited by l-threonine plus l-lysine. the homoserine dehydrogenase was nadp-specific and was inhibited by l-threonine. separation of the two enzymes by ammonium sulphate fractionation was possible in acetobacter peroxydans, a. rancens and gluconobacter mela ...	1975	168808
[function of cytochrome c in a mutant of candida mycoderma yeasts lacking in cytochromes b and a+a3].	the mutant of candida mycoderma was studied, which lacked cytochromes b and a+a3. the mutant contained only cytochrome c which functioned with cytochrome c peroxidase in the absence of any cytochrome c oxidases.	1975	169457
food poisoning--four unusual episodes.	four unusual outbreaks of food poisoning occurring in the dunedin health district during the period 1971-1973 are described. these involved a contaminated cordial, a death associated with a clostridium perfringens outbreak, salmonellosis and infectious hepatitis in persons eating uncooked shellfish and symptoms associated with the ingestion of a normally edible fish--the trumpeter.	1975	170567
proceedings: factors affecting glycerol and dihydroxyacetone phosphorylation in acetobacter xylinum.		1975	173674
[effect of endogenous respiration of candida mycoderma on the operation of the cytochrome system and the oxidation of glucose, ethanol and acids of the krebs cycle].	the kinetics of oxidation of glucose, ethanol, and acids of the krebs cycle was studied in resting cells of candida mycoderma which differed by the level of endogenous respiration (er). the cells with er close to zero oxidized glucose and ethanol almost at once and at a high rate. during oxidation of succinic or other acids of the krebs cycle, qo2 was maximal only after a long period of adaptation (tad); a latent period (tlat) was observed sometimes during which assimilation of oxygen was either ...	1975	175244
isolation and study of levansaccharase of acetobacter subyoxydans l-1.		1975	1732
pyruvate, orthophosphate dikinase from acetobacter sylinum.		1975	237179
[remarks on ethanol oxidation by an "acetobacter xylinum" microbial electrode (author's transl)].	a "microbial electrode" for ethanol assay has been designed using combination of an oxygen probe and cellulosic pellicle of acetobacter xylinum. assay is feasible with an ethanol concentration below 0.4 mm on a ph range of 2,5-7. the formation of acetic acid leds to a competitive inhibition of ethanol oxidation as observed with free cells. pellicle stability at room temperature is good over a ten hours period. at 4 degrees c, film preservation is quite satisfactory over a ten days storage period ...	1975	239620
studies on the activated sludge bacteria participating in the biodegradation of methanol, formaldehyde and ethylene glycol. i. isolation and identification.		1974	4209888
studies on the activated sludge bacteria participating in the biodegradation of methanol, formaldehyde and ethylene glycol. ii. utilization of various carbon and nitrogen compounds.		1974	4209889
myo-inositol dehydrogenase(s) from acetomonas oxydans. optimization of conditions for solubilization of membrane-bound enzyme.	methods are described for the first reported successful isolation of the soluble form of the membrane-bound myo-inositol dehydrogenase(s) from acetomonas oxydans. conditions for optimum yields of active enzyme in a crude membrane-free protein extract were established. the relevant conditions are (a) cell rupture by solid-shearing, (b) solubilization of the complete 60000g pellet with sodium deoxycholate at ph7.2, (c) rapid separation of the released protein from sodium deoxycholate by gel chroma ...	1974	4214535
transformation of organic compounds by microbial enzymes.		1974	4214719
growth inhibition of acetobacter aceti by l-threonine and l-homoserine: the primary regulation of the biosynthesis of amino acids of the aspartate family.		1974	4373525
specificity of glycerol kinase.	the activity of a number of alcohols was examined as substrates or inhibitors of glycerol kinase (atp-glycerol phosphotransferase; ec 2.7.1.30) from candida mycoderma. on the basis of these and other results, a modified model is proposed to account for the substrate specificity of the enzyme.	1974	4375973
[incorporation of cytochrome c from candida mycoderma yeasts into rat liver mitochondria].		1974	4377022
substrate specificity of an alpha-amino acid ester hydrolase produced by acetobacter turbidans a.t.c.c. 9325.	a partially purified preparation of an alpha-amino acid ester hydrolase was obtained from acetobacter turbidans a.t.c.c. 9325, which catalyses synthesis of 7-(d-alpha-amino-alpha-phenylacetamido)-3-cephem-3-methyl-4- carboxylic acid (cephalexin) from methyl d-alpha-aminophenylacetate and 7-amino-3-deacetoxycephalosporanic acid. the enzyme preparation catalysed both cephalosprin synthesis from 7-amino-3-deacetoxycephalosporanic acid and suitable amino acid esters (e.g. methyl d-alpha-aminophenyla ...	1974	4424889
regulation of hexose phosphate metabolism in acetobacter xylinum.	the metabolism of glucose and fructose was studied in resting succinate-grown cells of acetobacter xylinum. from fructose only cellulose and co(2) were formed by the cells, whereas from glucose, gluconate was formed much more rapidly than these two products. the molar ratio of sugar converted into cellulose to sugar converted into co(2) was significantly greater than unity for both hexoses. the pattern of label retention in the cellulose formed by the cells from specifically (14)c-labelled gluco ...	1974	4429547
stability of commensalistic systems.		1974	4429794
proceedings: the effect of some single amino acids on the growth of acetobactet aceti.		1974	4444831
fermentative production of l-sorbose from d-sorbitol by acetobacter suboxydans (vinegar isolate).		1974	4448494
carbon isotopic fractionations associated with acetic acid production by acetobacter suboxydans.		1974	4462877
[dihydroxyacetone preparation via glycerin oxidation by a suspension of resting acetobacter suboxydans cells].		1974	4463353
the structure of spherulites of bacterial cellulose from acetobacter xylinum.		1974	4597644
polysaccharide biosynthesis in acetobacter xylinum. enzymatic synthesis of lipid diphosphate and monophospate sugars.		1974	4600325
[thin layer electrophoretic determination of biogenic amines in fishes and fish products in connection with food poisoning].		1974	4616180