Publications

the metal centers of particulate methane monooxygenase from methylosinus trichosporium ob3b.	particulate methane monooxygenase (pmmo) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria. the nature of the pmmo active site and the overall metal content are controversial, with spectroscopic and crystallographic data suggesting the presence of a mononuclear copper center, a dinuclear copper center, a trinuclear center, and a diiron center or combinations thereof. most studies have focused on pmmo from methylococcus capsulatus (bath). pmmo from a s ...	2008	18540635
effect of temperature and pressure on growth and methane utilization by several methanotrophic cultures.	several methanotrophic microorganisms, i.e., methylococcus capsulatus (bath), methylomonas albus (bg-8), methylosinus trichosporium ob3b, and methylocystis parvus (obbp), were evaluated for growth and methane utilization. the effect of temperature was examined in the range of 25 to 45 degrees c for growth and methane utilization. the temperature variations (25-35 degrees c) had minimal effect on growth of m. albus and m. parvus. methane consumption varied at different temperatures with a maximum ...	1998	18576037
the fine structure of methylococcus capsulatus.	the fine structure of methylococcus capsulatus is described. particular emphasis is focused on the intracytoplasmic membrane system which is organized as a stacked array of flattened saccules. each saccule is limited by a 75 a unit membrane and lies in close apposition to adjacent saccules. methylococcus capsulatus is an obligate methylotroph whose sole source of carbon and energy is methane (or methanol). in this study methane oxidation is demonstrated for the first time in a cell-free system. ...	1970	18631529
transcription of nitrification genes by the methane-oxidizing bacterium, methylococcus capsulatus strain bath.	methylococcus capsulatus strain bath, a methane-oxidizing bacterium, and ammonia-oxidizing bacteria (aob) carry out the first step of nitrification, the oxidation of ammonia to nitrite, through the intermediate hydroxylamine. aob use hydroxylamine oxidoreductase (hao) to produce nitrite. m. capsulatus bath was thought to oxidize hydroxylamine with cytochrome p460 (cytl), until the recent discovery of an hao gene in its genome. we used quantitative pcr analyses of cdna from m. capsulatus bath inc ...	2008	18650926
the presence of multiple c-type cytochromes at the surface of the methanotrophic bacterium methylococcus capsulatus (bath) is regulated by copper.	identification of surface proteins is essential to understand bacterial communication with its environment. analysis of the surface-associated proteins of methylococcus capsulatus (bath) revealed a highly dynamic structure responding closely to the availability of copper in the medium in the range from approximately 0 to 10 microm. several c-type cytochromes, including three novel multihaem proteins, are present at the cellular surface, a feature that is otherwise a peculiarity of dissimilatory ...	2008	18681943
[purification and properties of membrane-bound methane hydroxylase from methylococcus capsulatus (strain m)].	membrane fraction of methylococcus capsulatus (strain m) were treated with [14c]acetylene, an affinity label binding to the active center of membrane-bound methane monooxygenase (mmo). high-purity particulate form of methane hydroxylase (pmh) was obtained by ion exchange and hydrophobic chromatography. according to sds-page data, the enzyme contained three polypeptides with molecular weights of 47 (alpha), 27 (beta), and 25 (gamma) kda in the ratio 1:1:1. the radiolabel was contained in the beta ...	2008	18946992
using shotgun sequence data to find active restriction enzyme genes.	whole genome shotgun sequence analysis has become the standard method for beginning to determine a genome sequence. the preparation of the shotgun sequence clones is, in fact, a biological experiment. it determines which segments of the genome can be cloned into escherichia coli and which cannot. by analyzing the complete set of sequences from such an experiment, it is possible to identify genes lethal to e. coli. among this set are genes encoding restriction enzymes which, when active in e. col ...	2009	18988632
the metal centres of particulate methane mono-oxygenase.	pmmo (particulate methane mono-oxygenase) is an integral membrane metalloenzyme that catalyses the oxidation of methane to methanol. the pmmo metal active site has not been identified, precluding detailed investigation of the reaction mechanism. models for the metal centres proposed by various research groups have evolved as crystallographic and spectroscopic data have become available. the present review traces the evolution of these active-site models before and after the 2005 methylococcus ca ...	2008	19021511
the methane monooxygenase intrinsic activity of kinds of methanotrophs.	methanotrophs have promising applications in the epoxidation of some alkenes and some chlorinated hydrocarbons and in the production of a biopolymer, poly-beta-hydroxybutyrate (poly-3-hydroxybutyrate; phb). in contrast with methane monooxygenase (mmo) activity and ability of phb synthesis of four kinds of methanotrophic bacteria methylosinus trichosporium ob3b, m. trichosporium imv3011, methylococcus capsulatus hd6t, methylomonas sp. gyj3, and the mixture of the four kinds of strains, m. trichos ...	2009	19052919
characterization of the pyrophosphate-dependent 6-phosphofructokinase from methylococcus capsulatus bath.	an active pyrophosphate-dependent 6-phosphofructokinase (ppi-pfk) from the thermotolerant methanotroph methylococcus capsulatus bath, containing a six-residue polyhistidine tag, was characterized. the enzyme was homodimeric (2 x 45 kda), nonallosteric and most active at ph 7.0. ppi-pfk catalyzed reactions of ppi-dependent phosphorylation of fructose-6-phosphate (f-6-p) (k(m) 2.27 mm and v(max) 7.6 u mg(-1) of protein), sedoheptulose-7-phosphate (k(m) 0.027 mm and v(max) 31 u mg(-1)) and ribulose ...	2008	19054082
activity and diversity of methanotrophs in the soil-water interface and rhizospheric soil from a flooded temperate rice field.	to combine molecular and cultivation techniques to characterize the methanotrophic community in the soil-water interface (swi) and rhizospheric soil from flooded rice fields in uruguay, a temperate region in south america.	2009	19054233
phylogenetic analysis of the triterpene cyclase protein family in prokaryotes and eukaryotes suggests bidirectional lateral gene transfer.	functional constraints to modifications in triterpene cyclase amino acid sequences make them good candidates for evolutionary studies on the phylogenetic relatedness of these enzymes in prokaryotes as well as in eukaryotes. in this study, we used a set of identified triterpene cyclases, a group of mainly bacterial squalene cyclases and a group of predominantly eukaryotic oxidosqualene cyclases, as seed sequences to identify 5288 putative triterpene cyclase homologues in publicly available databa ...	2009	19207562
development and validation of promoter-probe vectors for the study of methane monooxygenase gene expression in methylococcus capsulatus bath.	a series of integrative and versatile broad-host-range promoter-probe vectors carrying reporter genes encoding green fluorescent protein (gfp), catechol 2,3-dioxygenase (xyle) or beta-galactosidase (lacz) were constructed for use in methanotrophs. these vectors facilitated the measurement of in vivo promoter activity in methanotrophs under defined growth conditions. they were tested by constructing transcriptional fusions between the soluble methane monooxygenase (smmo) sigma(54) promoter or par ...	2009	19246747
structure of the redox sensor domain of methylococcus capsulatus (bath) mmos.	mmos from methylococcus capsulatus (bath) is the multidomain sensor protein of a two-component signaling system proposed to play a role in the copper-mediated regulation of soluble methane monooxygenase (smmo). mmos binds an fad cofactor within its n-terminal tandem per-arnt-sim (pas) domains, suggesting that it functions as a redox sensor. the crystal structure of the mmos tandem pas domains, designated pas-a and pas-b, has been determined to 2.34 a resolution. both domains adopt the typical pa ...	2009	19271777
rice roots select for type i methanotrophs in rice field soil.	methanotrophs are an important regulator for reducing methane (ch(4)) emissions from rice field soils. the type i group of the proteobacterial methanotrophs are generally favored at low ch(4) concentration and high o(2) availability, while the type ii group lives better under high ch(4) and limiting o(2) conditions. such physiological differences are possibly reflected in their ecological preferences. in the present study, methanotrophic compositions were compared between rice-planted soil and n ...	2009	19481894
diversity and activity of methanotrophs in alkaline soil from a chinese coal mine.	culture-independent molecular biological techniques, including 16s rrna gene and functional gene clone libraries and microarray analyses using pmoa (encoding a key subunit of particulate methane monooxygenase), were applied to investigate the methanotroph community structure in alkaline soil from a chinese coal mine. this environment contained a high diversity of methanotrophs, including the type ii methanotrophs methylosinus/methylocystis, type i methanotrophs related to methylobacter/methyloso ...	2009	19515201
microbial diversity in sediments associated with a shallow methane seep in the tropical timor sea of australia reveals a novel aerobic methanotroph diversity.	this study examined the diversity of bacteria, archaea and in particular aerobic methanotrophs associated with a shallow (84 m) methane seep in the tropical timor sea, australia. seepage of thermogenic methane was associated with a large carbonate hardground covered in coarse carbonate-rich sediments and various benthic organisms such as solitary corals. the diversity of bacteria and archaea was studied by analysis of cloned 16s rrna genes, while aerobic methanotrophic bacteria were quantified u ...	2009	19573197
aptamer-mediated turn-on fluorescence assay for prion protein based on guanine quenched fluophor.	an aptamer-participated haprin structure was designed by employing cellular prion protein (prp(c)) as a model protein, and thus an aptamer-mediated turn-on fluorescence assay for proteins was developed in this contribution. the designed aptamer-participated haprin structure consists of three segments. namely, an aptamer sequence located in the loop, three guanine bases at 3'-terminal, and a fluophor modified at 5'-terminal. it was found that the guanine bases at the 3'-terminal could quench the ...	2009	19635360
[specification of species status of some colleciton strains of methanotrophs].	sequence-analysis of genes 16s rrna has demonstrated the high-level relationship (99%) of the strains methylobacter ucrainicus ucm b-3159, and methylobacter marinus a45(t). the strain ucm b-3159 has lower coefficients of similarity (97.4-96.4%) for other species of that genus. these strains are similar as to their phenotypical properties and form one branch on the dendrogram which demonstrates species relations of methylococcaceae family, that permitted reclassifying m. ucrainicus as m. marinus. ...	2009	19663320
revisiting the mechanism of dioxygen activation in soluble methane monooxygenase from m. capsulatus (bath): evidence for a multi-step, proton-dependent reaction pathway.	stopped-flow kinetic investigations of soluble methane monooxygenase (smmo) from m. capsulatus (bath) have clarified discrepancies that exist in the literature regarding several aspects of catalysis by this enzyme. the development of thorough kinetic analytical techniques has led to the discovery of two novel oxygenated iron species that accumulate in addition to the well-established intermediates h(peroxo) and q. the first intermediate, p*, is a precursor to h(peroxo) and was identified when th ...	2009	19921958
remediation of chromium(vi) by a methane-oxidizing bacterium.	methane-oxidizing bacteria are ubiquitous in the environment and are globally important in oxidizing the potent greenhouse gas methane. it is also well recognized that they have wide potential for bioremediation of organic and chlorinated organic pollutants, thanks to the wide substrate ranges of the methane monooxygenase enzymes that they produce. here we have demonstrated that the well characterized model methanotroph methylococcus capsulatus (bath) is able to bioremediate chromium(vi) polluti ...	2010	20039753
oxidation of methane by a biological dicopper centre.	vast world reserves of methane gas are underutilized as a feedstock for the production of liquid fuels and chemicals owing to the lack of economical and sustainable strategies for the selective oxidation of methane to methanol. current processes to activate the strong c-h bond (104 kcal mol(-1)) in methane require high temperatures, are costly and inefficient, and produce waste. in nature, methanotrophic bacteria perform this reaction under ambient conditions using metalloenzymes called methane ...	2010	20410881
biochemistry: getting the metal right.		2010	20445616
physiological evidence for the presence of a cis-trans isomerase of unsaturated fatty acids in methylococcus capsulatus bath to adapt to the presence of toxic organic compounds.	the physiology of the response in the methanotrophic bacterium methylococcus capsulatus bath towards thermal and solvent stress was studied. a systematic investigation of the toxic effects of organic compounds (chlorinated phenols and alkanols) on the growth of this bacterium was carried out. the sensitivity to the tested alkanols correlated with their chain length and hydrophobicity; methanol was shown to be an exception to which the cells showed a very high tolerance. this can be explained by ...	2010	20487020
identification of a bacterial di-haem cytochrome c peroxidase from methylomicrobium album bg8.	the nucleotide sequence of an open reading frame (corb) downstream of the copper-repressible cora-encoding gene of the methanotrophic bacterium methylomicrobium album bg8 was obtained by restriction enzyme digestion and inverse pcr. the amino acid sequence deduced from this gene showed significant sequence similarity to the surface-associated di-haem cytochrome c peroxidase (saccp) previously isolated from methylococcus capsulatus (bath), including both c-type haem-binding motifs. homology analy ...	2010	20576687
evaluation of methane-utilising bacteria products as feed ingredients for monogastric animals.	bacterial proteins represent a potential future nutrient source for monogastric animal production because they can be grown rapidly on substrates with minimum dependence on soil, water, and climate conditions. this review summarises the current knowledge on methane-utilising bacteria as feed ingredients for animals. we present results from earlier work and recent findings concerning bacterial protein, including the production process, chemical composition, effects on nutrient digestibility, meta ...	2010	20578647
characterization of recombinant fructose-1,6-bisphosphate aldolase from methylococcus capsulatus bath.	the gene fba from the thermotolerant obligate methanotroph methylococcus capsulatus bath was cloned and expressed in escherichia coli bl21(de3). the fructose-1,6-bisphosphate aldolase (fba) carrying six his on the c-end was purified by affinity metal chelating chromatography. the mc. capsulatus fba is a hexameric enzyme (240 kda) that is activated by co2+ and inhibited by edta. the enzyme displays low k(m) to fructose-1,6-bisphosphate (fbp) and higher k(m) to the substrates of aldol condensation ...	2010	20673213
[effects of rice straw on the diversity of nitrifying genes (amoa and hao) in paddy soil].	the effects of long-term (16 years) fertilization on the diversity and community structure of soil ammonia-oxidizing gene (amoa) and hydroxylamine-oxidizing gene (hao) in paddy soil were evaluated using the methods of polymerase chain reaction, cloning and sequencing. the soil samples were collected from the treatments of npk (ck) and npk plus rice straw (sr) of the long-term field fertilization experiment in taoyuan agro-ecological experimental station. the shannon indices showed that the diver ...	2010	20698282
spectral and thermodynamic properties of methanobactin from γ-proteobacterial methane oxidizing bacteria: a case for copper competition on a molecular level.	methanobactin (mb) is a low molecular mass copper-binding molecule analogous to iron-binding siderophores. the molecule is produced by many methanotrophic or methane oxidizing bacteria (mob), but has only been characterized to date in one mob, methylosinus trichosporium ob3b. to explore the potential molecular diversity in this novel class of metal binding compound, the spectral (uv-visible, fluorescent, and electron paramagnetic resonance) and thermodynamic properties of mb from two γ-proteobac ...	2010	20817303
secretion of flavins by three species of methanotrophic bacteria.	we detected flavins in the growth medium of the methanotrophic bacterium methylocystis species strain m. flavin secretion correlates with growth stage and increases under iron starvation conditions. two other methanotrophs, methylosinus trichosporium ob3b and methylococcus capsulatus (bath), secrete flavins, suggesting that flavin secretion may be common to many methanotrophic bacteria.	2010	20833792
characterization of recombinant pyrophosphate-dependent 6-phosphofructokinase from halotolerant methanotroph methylomicrobium alcaliphilum 20z.	pyrophosphate-dependent 6-phosphofructokinase (ppi-pfk) was obtained as his₆-tagged protein by cloning of the pfp gene from the aerobic obligate methanotroph methylomicrobium alcaliphilum 20z and characterized. the recombinant ppi-pfk (4×45 kda) was highly active, non-allosteric and stringently specific to pyrophosphate as the phosphoryl donor. the enzyme was more specific for the reverse reaction substrate fructose-1,6-bisphosphate (k(m) 0.095 mm, v(max) 805 u/mg of protein) than for the forwar ...	2010	20868748
plasticicumulans acidivorans gen. nov., sp. nov., a polyhydroxyalkanoate-accumulating gammaproteobacterium from a sequencing-batch bioreactor.	here we describe a novel bacterium, strain tud-yj37(t), which can accumulate more than 85 wt% of polyhydroxybutyrate (phb). the bacterium was isolated from a mixed-culture bioreactor by using a 'feast-famine' regime and its properties were characterized. phylogenetic analysis based on full 16s rdna gene sequences reveals that the isolate is a member of the gammaproteobacteria, forming an independent deep phylogenetic lineage. it is most closely related to members of the genera methylocaldum, met ...	2010	20971833
growth performance and carcase quality in broiler chickens fed on bacterial protein grown on natural gas.	1. the effects of increasing concentrations (0, 40, 80 or 120 g/kg) of bacterial protein meal (bpm) and bacterial protein autolysate (bpa) grown on natural gas on growth performance and carcase quality in broiler chickens were examined. 2. adding bpm to diets reduced feed intake and improved gain: feed from 0 to 21 d and overall to 35 d, but did not significantly affect weight gain compared to the soybean meal based control diet. 3. increasing concentrations of bpa significantly reduced growth r ...	2010	21058073
bacteria grown on natural gas prevent soybean meal-induced enteritis in atlantic salmon.	dietary inclusion of solvent extracted soybean meal (sbm) is associated with inflammation in the distal intestine of salmonid fish, commonly referred to as sbm-induced enteritis. the enteritis is linked to alcohol soluble components in sbm, but the mechanisms have not been established. previous studies show that bacterial meal (bm) containing mainly methylococcus capsulatus grown on natural gas is a suitable protein source for salmonids. the bm is rich in nucleotides, phospholipids, and small pe ...	2010	21106922
methylogaea oryzae gen. nov., sp. nov., a novel mesophilic methanotroph from a rice paddy field in uruguay.	a novel methanotroph, strain e10(t), was isolated from a rice paddy field in uruguay. strain e10(t) grows on methane or methanol as its sole carbon and energy source. it is a gram-negative, non-motile, non-pigmented, slightly curved rods showing type i intracytoplasmic membranes arranged in stacks. it is neutrophilic and mesophilic (optimum growth at 30-35 °c and with no growth above 37°c). the strain only possesses a particulate methane monooxygenase (pmoa). phylogenetic analysis based on the 1 ...	2010	21131502
diversity of methanotrophs in zoige wetland soils under both anaerobic and aerobic conditions.	zoige wetland is one of the most important methane emission centers in china. the oxidation of methane in the wetland affects global warming, soil ecology and atmospheric chemistry. despite their global significance, microorganisms that consume methane in zoige wetland remain poorly characterized. in this study, we investigated methanotrophs diversity in soil samples from both anaerobic site and aerobic site in zoige wetland using pmoa gene as a molecular marker. the cloning library was construc ...	2010	21179963
characterization of the recombinant pyrophosphate-dependent 6-phosphofructokinases from methylomicrobium alcaliphilum 20z and methylococcus capsulatus bath.	the embden-meyerhof-parnas (emp) glycolysis is the starting point of the core carbon metabolism. aerobic methanotrophs possessing activity of the pyrophosphate-dependent 6-phosphofructokinase (ppi-pfk) instead of the classical glycolytic enzyme atp-dependent 6-phosphofructokinase (atp-pfk) are promising model bacteria for elucidation of the role of inorganic pyrophosphate (ppi) and ppi-dependent glycolysis in microorganisms. characterization of the his(6)-tagged ppi-pfks from two methanotrophs, ...	2011	21419911
methylococcus capsulatus (bath) from genome to protein function, and vice versa.	the genome sequence of methylococcus capsulatus (bath), considered a model methylotroph, was published in 2004 [ward, n., et al. (2004). genomic insights into methanotrophy: the complete genome sequence of methylococcus capsulatus (bath). plos biol.2, e303]. in the postgenomic era, the challenge is to determine the gene function, and to this end, genomics must be complemented with proteomic approaches. this chapter describes some experimental and computational approaches we have used and develop ...	2011	21419915
methanotroph outer membrane preparation.	all presently known methanotrophs are gram-negative bacteria suggesting that they are surrounded by a two-layered membrane: an inner or cytoplasmic membrane and an outer membrane. in the methanotroph methylococcus capsulatus (bath), separation of the two membranes has allowed studies on protein and lipid composition of the outer membrane. its outer membrane can be isolated from purified cell envelopes by selective solubilization of the inner membranes with the detergent triton x-100. the protein ...	2011	21419921
overexpression and purification of the particulate methane monooxygenase from methylococcus capsulatus (bath).	the particulate methane monooxygenase (pmmo) is a multi-copper enzyme that mediates the facile conversion of methane to methanol in methanotrophic bacteria. as a membrane-bound multi-subunit metalloprotein, the highly active protein has been difficult to isolate and purify to homogeneity for biochemical and biophysical studies. in this chapter, we describe a method to overexpress pmmo with good specific activity in high yields in the intracytoplasmic membranes of the host organism, together with ...	2011	21419922
metal reconstitution of particulate methane monooxygenase and heterologous expression of the pmob subunit.	particulate methane monooxygenase (pmmo) is a multisubunit metalloenzyme complex used by methanotrophic bacteria to oxidize methane in the first step of carbon assimilation and energy production. in this chapter, we detail methods to prepare metal free (apo) membrane-bound pmmo and to reconstitute apo pmmo with metal ions. we also describe protocols to clone, express, and refold metal-loaded soluble domain constructs of the pmob subunit. these approaches were used to address fundamental question ...	2011	21419923
isolation of methanobactin from the spent media of methane-oxidizing bacteria.	chalkophores are low molecular mass modified peptides involved in copper acquisition in methane-oxidizing bacteria (mob). a screening method for the detection of this copper-binding molecule is presented in chapter 16. here we describe methods to (1) maximize expression and secretion of chalkophores, (2) concentrate chalkophores from the spent media of mob, and (3) purify chalkophores.	2011	21419927
insights into the different dioxygen activation pathways of methane and toluene monooxygenase hydroxylases.	the methane and toluene monooxygenase hydroxylases (mmoh and tmoh, respectively) have almost identical active sites, yet the physical and chemical properties of their oxygenated intermediates, designated p, h(peroxo), q, and q in mmoh and tomoh(peroxo) in a subclass of tmoh, tomoh, are substantially different. we review and compare the structural differences in the vicinity of the active sites of these enzymes and discuss which changes could give rise to the different behavior of h(peroxo) and ...	2011	21517016
model of the molecular basis for hydroxylamine oxidation and nitrous oxide production in methanotrophic bacteria.	many methane-oxidizing bacteria (mob) have been shown to aerobically oxidize ammonia and hydroxylamine (nh(2)oh) to produce nitrite and nitrous oxide (n(2)o). genome sequences of alphaproteobacterial, gammaproteobacterial, and verrucomicrobial methanotrophs revealed the presence of haoab, cytl, cyts, nirs or nirk, and norcb genes that may be responsible for n(2)o production, and additional haoab genes were sequenced from two strains of methylomicrobium album. the haoab genes of m. album atcc 330 ...	2011	21682764
[effect of long-term application of nitrogen fertilizer on the diversity of nitrifying genes (amoa and hao) in paddy soil].	the aim of this study was to determine the effect of long-term (16 years) application of nitrogen fertilizer on the diversity of nitrifying genes (amoa and hao) in paddy soil on the basis of long-term paddy field experimental station (started in 1990) located in taoyuan, with the molecular approaches of pcr, constructing libraries and sequencing. the fertilizer was urea and no fertilizer was as control. the shannon index showed that long-term application of nitrogen fertilizer made the diversity ...	2011	21780610
cytochrome c-554 from methylosinus trichosporium ob3b; a protein that belongs to the cytochrome c2 family and exhibits a hals-type epr signal.	a small soluble cytochrome c-554 purified from methylosinus trichosporium ob3b has been purified and analyzed by amino acid sequencing, mass spectrometry, visible, cd and epr spectroscopies. it is found to be a mono heme protein with a characteristic cytochrome c fold, thus fitting into the class of cytochrome c(2), which is the bacterial homologue of mitochondrial cytochrome c. the heme iron has a histidine/methionine axial ligation and exhibits a highly anisotropic/axial low spin (hals) epr si ...	2011	21789203
fatty acid composition, oxidative stability and sensory quality of meat from broiler chicken fed autolysate from bacteria grown on natural gas.	bacterial autolysate, a down stream product of bacterial biomass grown on natural gas by mainly the methanotrophic bacteria methylococcus capsulatus, was fed at 8% as is to broiler chickens from 1 to 35ôçâdays of age for studies of fatty acid composition, lipid oxidation and sensory quality of thigh meat stored frozen for 6ôçâmonth at -18ôçâ-¦c or -80ôçâ-¦c. lipid oxidation was measured by thiobarbituric acid reactive substances (tbars) and volatile profile by dynamic headspace gas chromatograph ...	2011	21831229
A metagenomic study of methanotrophic microorganisms in Coal Oil Point seep sediments.	Methane oxidizing prokaryotes in marine sediments are believed to function as a methane filter reducing the oceanic contribution to the global methane emission. In the anoxic parts of the sediments, oxidation of methane is accomplished by anaerobic methanotrophic archaea (ANME) living in syntrophy with sulphate reducing bacteria. This anaerobic oxidation of methane is assumed to be a coupling of reversed methanogenesis and dissimilatory sulphate reduction. Where oxygen is available aerobic metha ...	2011	21970369
Crystal structure and characterization of particulate methane monooxygenase from Methylocystis species strain M.	Particulate methane monooxygenase (pMMO) is an integral membrane metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria. Previous biochemical and structural studies of pMMO have focused on preparations from Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3b. A pMMO from a third organism, Methylocystis species strain M, has been isolated and characterized. Both membrane-bound and solubilized Methylocystis sp. strain M pMMO contain ~2 copper ions per 100 kDa pr ...	2011	22013879
the copper responding surfaceome of methylococccus capsulatus bath.	proteins on the cellular surface of a bacterium, its surfaceome, are part of the interface between the bacterium and its environment, and are essential for the cells response to its habitat. methylococcus capsulatus bath is one of the most extensively studied methane-oxidizers and is considered as a model-methanotroph. the composition of proteins of the surfaceome of m. capsulatus bath varies with the availability of copper and changes significantly upon only minor changes of copper concentratio ...	2011	22092708
response to mercury (ii) ions in methylococcus capsulatus (bath).	the mercury (ii) ion is toxic and is usually detoxified in bacteria by reduction to elemental mercury, which is less toxic. this is catalysed by an nad(p)h-dependent mercuric reductase (ec 1.16.1.1). here, we present strong evidence that methylococcus capsulatus (bath) - a methanotrophic member of the gammaproteobacteria - uses this enzyme to detoxify mercury. in radiorespirometry studies, it was found that cells exposed to mercury dissimilated 100% of [(14) c]-methane provided to generate reduc ...	2011	22092810
community structure, abundance, and activity of methanotrophs in the zoige wetland of the tibetan plateau.	the zoige wetland of the tibetan plateau is a high-altitude tundra wetland and one of the biggest methane emission centers in china. in this study, methanotrophs with respect to community structure, abundance, and activity were investigated in peat soils collected in the vicinity of different marshland plants that dominate different regions of the wetland, including polygonum amphibium, carex muliensis, and eleocharis valleculosa (ev). 16s rrna gene and particulate methane monooxygenase gene (pm ...	2011	22159497