| engineering methylobacterium extorquens for de novo synthesis of the sesquiterpenoid α-humulene from methanol. | over the last 10 to 15 years, metabolic engineering of microbes has become a versatile tool for high-level de novo synthesis of terpenoids, with the sesquiterpenoids armopha-1,4-diene, farnesene and artemisinic acid as prime examples. however, almost all cell factory approaches towards terpenoids to date have been based on sugar as the raw material, which is mainly used as a food resource and subject to high price volatilities. in this study we present de novo synthesis of the sesquiterpenoid α- ... | 2015 | 26369439 |
| metabolic footprint of epiphytic bacteria on arabidopsis thaliana leaves. | the phyllosphere, which is defined as the parts of terrestrial plants above the ground, is a large habitat for different microorganisms that show a high extent of adaption to their environment. a number of hypotheses were generated by culture-independent functional genomics studies to explain the competitiveness of specialized bacteria in the phyllosphere. in contrast, in situ data at the metabolome level as a function of bacterial colonization are lacking. here, we aimed to obtain new insights ... | 2016 | 26305156 |
| [functional activity of the moda, gene in methylobacterium dichloromethanicum dm4]. | the putative metdi2644 (moda2) gene of methylobacterium dichloromethanicum dm4, present in the 126-kbp chromosomal fragment associated with dichloromethane (dcm) degradation was investigated. while this gene is presumed to encode the periplasmic substrate-binding subunit of the molybdate abc transporter, its conceptual translation also exhibits similarity to the proteins containing the osta conservative domain and responsible for resistance of gram-negative bacteria to organic solvents. reverse ... | 2015 | 25844439 |
| [genetic modification of methylobacterium extorquens g10 producer strain of polyhydroxybutyrate]. | the effect of the increased copy number of polyhydroxybutyrate (phb) biosynthesis genes in pink-pigmented methylobacterium methylobacterium extorquens g10 on properties of the biopolymer was studied. the activity of poly-3-hydroxybutyril-synthase (phb-synthase) was shown to increase and the molecular weight of synthesized phb decreases twofold (150 --> 79 kda) after insertion of extra copies of phac and phacab genes into cells of the producer strain, whereas the physicochemical properties of the ... | 2015 | 25757337 |
| electro-biocatalytic production of formate from carbon dioxide using an oxygen-stable whole cell biocatalyst. | the use of biocatalysts to convert co2 into useful chemicals is a promising alternative to chemical conversion. in this study, the electro-biocatalytic conversion of co2 to formate was attempted with a whole cell biocatalyst. eight species of methylobacteria were tested for co2 reduction, and one of them, methylobacterium extorquens am1, exhibited an exceptionally higher capability to synthesize formate from co2 by supplying electrons with electrodes, which produced formate concentrations of up ... | 2015 | 25746476 |
| high-level production of ethylmalonyl-coa pathway-derived dicarboxylic acids by methylobacterium extorquens under cobalt-deficient conditions and by polyhydroxybutyrate negative strains. | bio-based production of dicarboxylic acids is an emerging research field with remarkable progress during the last decades. the recently established synthesis of the ethylmalonyl-coa pathway (emcp)-derived dicarboxylic acids, mesaconic acid and (2s)-methylsuccinic acid, from the alternative carbon source methanol (sonntag et al., appl microbiol biotechnol 98:4533-4544, 2014) gave a proof of concept for the sustainable production of hitherto biotechnologically inaccessible monomers. in this study, ... | 2015 | 25661812 |
| methylobacterium extorquens: methylotrophy and biotechnological applications. | methylotrophy is the ability to use reduced one-carbon compounds, such as methanol, as a single source of carbon and energy. methanol is, due to its availability and potential for production from renewable resources, a valuable feedstock for biotechnology. nature offers a variety of methylotrophic microorganisms that differ in their metabolism and represent resources for engineering of value-added products from methanol. the most extensively studied methylotroph is the alphaproteobacterium methy ... | 2015 | 25432674 |
| discovery of rare protein-coding genes in model methylotroph methylobacterium extorquens am1. | proteogenomics involves the use of ms to refine annotation of protein-coding genes and discover genes in a genome. we carried out comprehensive proteogenomic analysis of methylobacterium extorquens am1 (me-am1) from publicly available proteomics data with a motive to improve annotation for methylotrophs; organisms capable of surviving in reduced carbon compounds such as methanol. besides identifying 2482(50%) proteins, 29 new genes were discovered and 66 annotated gene models were revised in me- ... | 2014 | 25158906 |
| interaction with ectomycorrhizal fungi and endophytic methylobacterium affects nutrient uptake and growth of pine seedlings in vitro. | tissues of scots pine (pinus sylvestris l.) contain several endophytic microorganisms of which methylobacterium extorquens dsm13060 is a dominant species throughout the year. similar to other endophytic bacteria, m. extorquens is able to colonize host plant tissues without causing any symptoms of disease. in addition to endophytic bacteria, plants associate simultaneously with a diverse set of microorganisms. furthermore, plant-colonizing microorganisms interact with each other in a species- or ... | 2014 | 25149086 |
| a set of versatile brick vectors and promoters for the assembly, expression, and integration of synthetic operons in methylobacterium extorquens am1 and other alphaproteobacteria. | the discipline of synthetic biology requires standardized tools and genetic elements to construct novel functionalities in microorganisms; yet, many model systems still lack such tools. here, we describe a novel set of vectors that allows the convenient construction of synthetic operons in methylobacterium extorquens am1, an important alphaproteobacterial model organism for methylotrophy and a promising platform organism for methanol-based biotechnology. in addition, we provide a set of constitu ... | 2015 | 25105793 |
| localization of strawberry (fragaria x ananassa) and methylobacterium extorquens genes of strawberry flavor biosynthesis in strawberry tissue by in situ hybridization. | strawberry flavor is one of the most popular fruit flavors worldwide, with numerous applications in the food industry. in addition, the biosynthetic origin of the most important strawberry flavor components, such as 2,5-dimethyl-4-hydroxy-2h-furan-3-one (dmhf), is a challenging research area. dmhf's precursor, 2-hydroxy-propanal (or lactaldehyde), is biosynthesized by the endophytic bacterium methylobacterium extorquens (m. extorquens). in particular, the alcohol dehydrogenase (adh) enzymes of m ... | 2014 | 24973582 |
| sign epistasis limits evolutionary trade-offs at the confluence of single- and multi-carbon metabolism in methylobacterium extorquens am1. | adaptation of one set of traits is often accompanied by attenuation of traits important in other selective environments, leading to fitness trade-offs. the mechanisms that either promote or prevent the emergence of trade-offs remain largely unknown, and are difficult to discern in most systems. here, we investigate the basis of trade-offs that emerged during experimental evolution of methylobacterium extorquens am1 to distinct growth substrates. after 1500 generations of adaptation to a multi-ca ... | 2014 | 24164359 |
| rare earth metals are essential for methanotrophic life in volcanic mudpots. | growth of methylacidiphilum fumariolicum solv, an extremely acidophilic methanotrophic microbe isolated from an italian volcanic mudpot, is shown to be strictly dependent on the presence of lanthanides, a group of rare earth elements (rees) such as lanthanum (ln), cerium (ce), praseodymium (pr) and neodymium (nd). after fractionation of the bacterial cells and crystallization of the methanol dehydrogenase (mdh), it was shown that lanthanides were essential as cofactor in a homodimeric mdh compar ... | 2014 | 24034209 |
| comprehensive discovery of 13c labeled metabolites in the bacterium methylobacterium extorquens am1 using gas chromatography-mass spectrometry. | herein, we report the identification of isotopically labeled metabolite peaks (or the lack of labeling) between sets of gc-ms data from methylobacterium extorquens am1. m. extorquens am1 is one of the best-characterized model organisms for the study of c1 metabolism in methylotrophic bacteria, a diverse group of microbes that can use reduced one-carbon (c1) sources, such as methanol and methane as a sole source for both energy generation and carbon assimilation. application of a match value (mv) ... | 2013 | 24007683 |
| [aerobic methylobacteria as the basis for a biosensor for dichloromethane detection]. | cells of dichloromethane (dchm) bacteria-destructors were immobilized by sorption on different types of membranes, which were fixed on the measuring surface of a ph-sensitive field transistor. the presence of dchm in the medium (0.6-8.8 mm) led to a change in the transistor's output signal, which was determined by the appearance of h+ ions in the medium due to dchm utilization by methylobateria. among four strains of methylobacteria--methylobacterium dichloromethanicum dm4, methylobacterium exto ... | 2013 | 23795481 |
| [biosynthesis of polyhydroxybutyrate/valerate with different molecular weights during the growth of methylobacterium extorquens g-10 on a methanol-pentanol mixture]. | the influence of the concentration and time of addition of cosubstrate (pentanol) on the molecular weight (mw) of the polyhydroxybutyrate/valerate (phbv) copolymer synthesized by methylobacterium extorquens g-10 during cultivation in a methanol-containing medium has been studied. it was shown that an increase in the pentanol concentration to 20% in a mixture with methanol stimulated the biosynthesis of phbv with a mw of approximately 1500 kda and increased the content of valerate up to 50%, espe ... | 2013 | 23795476 |
| single-domain response regulator involved in the general stress response of methylobacterium extorquens. | the general stress response of alphaproteobacteria is regulated by a partner-switching mechanism that involves the alternative sigma factor σ(ecfg), the anti-sigma factor nepr and the anti-sigma factor antagonist phyr. to address the question of how the phyr-nepr-σ(ecfg) cascade is activated and modulated in methylobacterium extorquens, a forward genetic screen was applied. the screen identified the single-domain response regulator mext_0407 as a novel regulatory element in the general stress re ... | 2013 | 23596318 |
| [functional analysis of the genome fragment involved in aerobic dichloromethane degradation by methylobacterium dichloromethanicum dm4]. | the hypothetical genes of methylobacterium dichloromethanicum dm4, metdi 2671 (biod2), and metdi 2680 located within the chromosomal fragment (126 kb) associated with dichloromethane (dcm) degradation have been studied. the reverse transcription polymerase chain reaction method (rt-pcr) showed the presence of transcripts of both genes in cells grown on dcm and methanol. the mobilized suicidal vector pk18mob was used to obtain knockout mutants in these genes. the bio mutant (with an insertion in ... | 2013 | 23101389 |
| gas chromatography-mass spectrometry with chemometric analysis for determining ¹²c and ¹³c labeled contributions in metabolomics and ¹³c flux analysis. | a novel method for the analysis of nearly co-eluting ¹²c and ¹³c isotopically labeled metabolites has been developed and evaluated for gas chromatography coupled to mass spectrometry (gc-ms) data. the method utilizes parallel factor analysis (parafac) with two-dimensional gc-ms data when sample replicates are aligned and stacked in series to create a three-dimensional data cube for mathematical peak deconvolution and ¹²c and ¹³c contribution isolation, with the intent of increasing the accuracy ... | 2012 | 22503618 |