TitleAbstractYear(sorted ascending)
cloning and nucleotide sequences of the homoserine dehydrogenase genes (hom) and the threonine synthase genes (thrc) of the gram-negative obligate methylotroph methylobacillus glycogenes.we have cloned the homoserine dehydrogenase genes (hom) from the gram-negative obligate methylotrophs methylobacillus glycogenes atcc 21276 and atcc 21371 by complementation of an escherichia coli homoserine dehydrogenase-deficient mutant. the 4.15-kb dna fragment cloned from m. glycogenes atcc 21371 also complemented an e. coli threonine synthase-deficient mutant, suggesting the dna fragment contained the thrc gene in addition to the hom gene. the homoserine dehydrogenases expressed in the e. c ...19948117070
purification and characterization of chaperonins 60 and 10 from methylobacillus glycogenes.two proteins belonging to the group i chaperonin family were isolated from an obligate methanotroph, methylobacillus glycogenes. the two proteins, one a groel homologue (cpn60: m. glycogenes 60 kda chaperonin) and the other a groes homologue (cpn10: m. glycogenes 10 kda chaperonin), composed a heteropolymeric complex in the presence of atp. both proteins were purified from crude extracts of m. glycogenes by anion-exchange (deae-toyopearl) and gel-filtration (sephacryl s-400) chromatography. the ...19989764760
distribution of aldoxime dehydratase in microorganisms.the distribution of phenylacetaldoxime-degrading and pyridine-3-aldoxime-degrading ability was examined with intact cells of 975 microorganisms, including 45 genera of bacteria, 11 genera of actinomyces, 22 genera of yeasts, and 37 genera of fungi, by monitoring the decrease of the aldoximes by high-pressure liquid chromatography. the abilities were found to be widely distributed in bacteria, actinomyces, fungi, and some yeasts: 98 and 107 strains degraded phenylacetaldoxime and pyridine-3-aldox ...200010831401
phylogenetic analysis of tmrna genes within a bacterial subgroup reveals a specific structural signature.bacterial tmrna mediates a trans-translation reaction, which permits the recycling of stalled ribosomes and probably also contributes to the regulated expression of a subset of genes. its action results in the addition of a small number of c-terminal amino acids to protein whose synthesis had stalled and these constitute a proteolytic recognition tag for the degradation of these incompletely synthesized proteins. previous work has identified pseudoknots and stem-loops that are widely conserved i ...200111266563
overproduction of l-lysine from methanol by methylobacillus glycogenes derivatives carrying a plasmid with a mutated dapa gene.the dapa gene, encoding dihydrodipicolinate synthase (ddps) partially desensitized to inhibition by l-lysine, was cloned from an l-threonine- and l-lysine-coproducing mutant of the obligate methylotroph methylobacillus glycogenes dhl122 by complementation of the nutritional requirement of an escherichia coli dapa mutant. introduction of the dapa gene into dhl122 and al119, which is the parent of dhl122 and an l-threonine producing mutant, elevated the specific activity of ddps 20-fold and l-lysi ...200111425723
tmrdb (tmrna database).maintained at the university of texas health science center at tyler, texas, the tmrna database (tmrdb) is accessible at the url with mirror sites located at auburn university, auburn, alabama ( and the bioinformatics research center, aarhus, denmark ( the tmrdb collects and distributes information relevant to the study of tmrna. in trans-translation, this molecule combines propert ...200312520048
methylotrophic autotrophy in beijerinckia mobilis.representatives of the genus beijerinckia are known as heterotrophic, dinitrogen-fixing bacteria which utilize a wide range of multicarbon compounds. here we show that at least one of the currently known species of this genus, i.e., beijerinckia mobilis, is also capable of methylotrophic metabolism coupled with the ribulose bisphosphate (rubp) pathway of c1 assimilation. a complete suite of dehydrogenases commonly involved in the sequential oxidation of methanol via formaldehyde and formate to c ...200515901717
cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.aspartokinase (ask) exists within a variable network that supports the synthesis of 9 amino acids and a number of other important metabolites. lysine, isoleucine, aromatic amino acids, and dipicolinate may arise from the ask network or from alternative pathways. ask proteins were subjected to cohesion group analysis, a methodology that sorts a given protein assemblage into groups in which evolutionary continuity is assured. two subhomology divisions, ask(alpha) and ask(beta), have been recognize ...200919946135
analysis and manipulation of aspartate pathway genes for l-lysine overproduction from methanol by bacillus methanolicus.we investigated the regulation and roles of six aspartate pathway genes in l-lysine overproduction in bacillus methanolicus: dapg, encoding aspartokinase i (aki); lysc, encoding akii; yclm, encoding akiii; asd, encoding aspartate semialdehyde dehydrogenase; dapa, encoding dihydrodipicolinate synthase; and lysa, encoding meso-diaminopimelate decarboxylase. analysis of the wild-type strain revealed that in vivo lysc transcription was repressed 5-fold by l-lysine and induced 2-fold by dl-methionine ...201121724876
the effect of carbon subsidies on marine planktonic niche partitioning and recruitment during biofilm assembly.the influence of resource availability on planktonic and biofilm microbial community membership is poorly understood. heterotrophic bacteria derive some to all of their organic carbon (c) from photoautotrophs while simultaneously competing with photoautotrophs for inorganic nutrients such as phosphorus (p) or nitrogen (n). therefore, c inputs have the potential to shift the competitive balance of aquatic microbial communities by increasing the resource space available to heterotrophs (more c) wh ...201526236289
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