Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| energy coupling efficiency of symbiotic nitrogen fixation. | 1977 | 562660 | |
| genetic control of symbiotic nitrogen fixation in soybeans. | 1977 | 562661 | |
| regulation of nitrogen fixation in rhizobium spp. isolation of mutants of rhizobium trifolii which induce nitrogenase activity. | this communication describes the isolation and characterization of mutants of rhizobium trifolii which can induce nitrogenase activity in defined liquid medium. two procedures were used for the isolation of these mutants from r. trifolii strain dt-6: (1) following chemical mutagenesis, slow growing mutants were selected which were unable to utilize nh+4 as sole source of nitrogen; (2) as spontaneous mutants resistant to the glutamate analogue l-methionine-dl-sulfoximine. mutants (dt-71, dt-125) ... | 1977 | 588592 |
| [production of inoculates for leguminous plants. production of cellular suspensions of rhizobium (lotus group)]. | the influence of culture medium composition and operative conditions on the cellular growth of rhizobium sp (group lotus) strain is studied. as much as 1 x 10(9) cell/ml were obtained in 16 hours using sucrose in the medium as carbon source. the best growth rate was obtained (mu = o,22 h-1) when the experiments were performed at 400 r.p.m. and one volume of air/volume of medium x minute (oar = 793,0 ml of oxygen/1 h). | 1977 | 594429 |
| [transformation of l-tryptophan by rhizobium phaseoli]. | transformation of l-tryptophan was studied in rhizobium phaseoli 680. the culture was capable of oxidation decarboxylation, transamination and degradation of l-tryptophan. four metabolites were identified: tryptamine, indolyl-3-pyruvic, indolyl-3-acetic and anthranilic acids. the fifth metabolite has not been yet identified. | 1977 | 600098 |
| [studies concerning the biology of rhizobia. 1. communication: serological investigations (author's transl)]. | according to earlier studies the strains of the rhizobia could be put into three biochemical groups (a, b, c) independently of the nodules of which kind of plants having been isolated from. the aim of our experiments has been the determination of the antigenic structure of these rhizobia strains. regarding the agglutination tests only 24 strains from the 47 ones were agglutinated by a sera, one b strain from the 3 b ones, and 2 c strains from the c ones gave positive reaction with b and c sera, ... | 1977 | 602469 |
| differential stimulation and inhibition of growth of rhizobium trifolii strain t1 and other rhizobium species by various carbon sources. | the physiological properties of rhizobium trifolii strain t1 were studied in detail, since this strain has many useful characteristics and appears ideal for development as a reference strain for r. trifolii. some tricarboxylic acid cycle intermediates and related compounds were found to stimulate growth in the presence of sucrose and arabinose, while others inhibited growth partially or completely. other r. trifolii strains behaved likewise. moreover, similar responses were also observed with ot ... | 1977 | 617211 |
| energy requirement for symbiotic nitrogen fixation. | comparing the energy required by legumes for symbiotic nitrogen fixation with that of assimilation of nitrate, gibson concluded the costs to be about the same. about 15% of net photosynthetic production by the plant may be used in meeting its nitrogen requirements. if energy for the fixation of nitrogen symbiotically and that for the assimilation of nh4+ or no3- from the soil solution are both provided by the chemical products of photosynthesis, then the co2 respired in supplying that energy mus ... | 1977 | 16073424 |
| root hair cell enhancement in tissue cultures from soybean roots: a useful model system: in vitro rhizobium symbiosis. | a technique for obtaining large numbers of root hair cells in cell cultures from soybeans is described. the cells were grown on agar containing the prairie regional laboratory b5 (prl-b5) medium for periods longer than 60 days. mixed populations of cultured root hair cells and cortical cells were used to study the in vitro association between soybean cells and rhizobium japonicum. the advantages of these types of root cell cultures in studies of symbiosis are discussed. | 1977 | 16659797 |
| receptor site on clover and alfalfa roots for rhizobium. | sites on white clover and alfalfa roots that bind rhizobium trifolii and r. meliloti capsular polysaccharides, respectively, were examined by fluorescence microscopy. fluorescein isothiocyanate-labeled capsular material from r. trifolii bound specifically to root hairs of clover but not alfalfa. binding was most intense at the root hair tips. treatment of clover roots with 2-deoxyglucose (2-dg) prevented binding of r. trifolii capsular material to the roots. the sugar 2-dg enhanced the elution o ... | 1977 | 16345183 |
| heme synthesis in soybean root nodules: i. on the role of bacteroid delta-aminolevulinic acid synthase and delta-aminolevulinic acid dehydrase in the synthesis of the heme of leghemoglobin. | during nodulation of soybean (glycine max) by rhizobium japonicum, variations in the activities of two enzymes of heme biosynthesis, delta-aminolevulinic acid synthase (alas) and delta-aminolevulinic acid dehydrase (alad) are described. delta-aminolevulinic acid synthase activity is found in the bacteroid fraction of nodules, but is not detected in the plant fraction. bacteroid alas activity parallels heme accumulation during nodule development. delta-aminolevulinic acid dehydrase activity is fo ... | 1977 | 16660108 |
| hydrogen reactions of nodulated leguminous plants: i. effect of rhizobial strain and plant age. | the atp-dependent evolution of h(2) catalyzed by nitrogenase and the hydrogenase-catalyzed oxidation of h(2) have been implicated as factors influencing the efficiency of energy utilization in the n(2) fixation process. the effects of rhizobial strain and plant age on the h(2)-evolving and h(2)-utilizing activity of leguminous root nodules are described in this manuscript. two classes of legume-rhizobium combinations were observed in studies with soybeans (glycine max l. merr.) and cowpeas (vign ... | 1977 | 16660157 |
| preparation and fractionation of rhizobium bacteroids by zone sedimentation through sucrose gradients. | zone sedimentation through sucrose gradients was used for preparing rhizobium bacteroids from lupin nodules and for separating them into slowly and rapidly sedimenting fractions.the purity of the bacteroids was established by electron microscopy and by enzyme assays, and they were shown to contain a co-insensitive cytochrome c oxidase.bacteroids sedimented more rapidly than broth-cultured rhizobium bacteria, and bacteroids from old nodules sedimented more rapidly than bacteroids from young nodul ... | 1977 | 16660187 |
| changes in the number, viability, and amino-acid-incorporating activity of rhizobium bacteroids during lupin nodule development. | between days 10 and 21 after inoculation of lupinus angustifolius seedlings with rhizobium nzp 2257, the average nodule fresh weight increased 3-fold and the number of bacteroids per nodule increased more than 10-fold.the viability of rhizobium bacteroids, as judged by their ability to form colonies on yeast-extract agar, declined from about 10% on days 10 and 11 after inoculation to about 0.3% on days 14 to 25. bacteroid viability was highly sensitive to osmolarity.at optimal ph and k and mg io ... | 1977 | 16659929 |
| role of lectins in plant-microorganism interactions: i. binding of soybean lectin to rhizobia. | highly purified soybean lectin (sbl) was labeled with fluorescein isothiocyanate (fitc-sbl) or tritium ((3)h-sbl) and repurified by affinity chromatography. fitc-sbl was found to bind to living cells of 15 of the 22 rhizobium japonicum strains tested. the lectin did not bind to cells of the other seven r. japonicum strains, or to cells of any of the nine rhizobium strains tested which do not nodulate soybean. the binding of the lectin to the sbl-positive strains of r. japonicum was shown to be s ... | 1977 | 16660121 |
| chemotaxis of rhizobium spp. to a glycoprotein produced by birdsfoot trefoil roots. | rhizobium spp. show chemotaxis to plant root exudates. a glycoprotein has been isolated from the root exudates of birdsfoot trefoil, lotus corniculatus, which, at micromolar concentrations, attracts six strains of rhizobia. this glycoprotein has been given the trivial name trefoil chemotactin and contains approximately twice as much protein as carbohydrate. gel filtration of trefoil chemotactin on a bio-gel a-1.5m column gave a molecular weight of approximately 60,000. trefoil chemotactin repres ... | 1977 | 17776952 |
| purification and characterisation of d-amino acid aminotransferase from rhizobium japonicum. | rhizobium japonicum has d-amino acid aminotransferase and alanine racemase activities. the d-amino-acid aminotransferase has been partially purified and characterized. this enzyme has a broad specificity and is very active with d-alpha-aminobutyrate and d-aspartate as well as d-alanine and d-glutamate. the stereospecificity of the enzyme for d-amino acids was apparently absolute with respect to product inhibition, pyridoxamine formation as well as catalytic activity. the apparent molecular weigh ... | 1978 | 620041 |
| pectolytic enzymes in rhizobium. | a sensitive pectin agar plate assay was used to demonstrate low levels of pectolytic enzymes in infective and noninfective strains of rhizobium. the possible relation of this characteristic to legume infection is discussed. | 1978 | 623465 |
| the effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by rhizobium leguminosarum. | the effects of nh4no3 on the development of root nodules of pisum sativum after infection with rhizobium leguminosarum (strain pre) and on the nitrogenase activity of the bacteroids in the nodule tissue were studied. the addition of nh4no3 decreased the nitrogenase activity measured on intact nodules. this reduction of nitrogen fixation did not result from a reduced number of bacteroids or a decreased amount of bacteroid proteins per gram of nodule. the synthesis of nitrogenase, measured as the ... | 1978 | 623788 |
| trifolin: a rhizobium recognition protein from white clover. | a protein agglutinin, trifoliin, was purified from white clover seeds and seedling roots. trifoliin specifically agglutinates the symbiont of clover, rhizobium trifolii, at concentrations as low as 0.2 microgram protein/ml, and binds to the surface of encapsulated r. trifolii 0403. this clover protein has a subunit with mr approximately 50 000, an isoelectric point of 7.3, and contains carbohydrate. antibody to purified trifoliin binds to the root hair region of 24-h-old clover seedlings, but do ... | 1978 | 630002 |
| inducing effect of plant cells on nitrogenase activity by spirillum and rhizobium in vitro. | eleven different plant cell tissue cultures of both legume and non-legume origin have been grown in direct association, and in separate but close proximal association with both spirillum lipoferum and rhizobium sp. 32h1. basic similarities were found in the nutritional requirement for the induction of nitrogenase activity (c2h2) in both organisms. in the absence of plant cell cultures both organisms need to be provided with a pentose sugar and a tricarboxylic acid to induce high levels of nitrog ... | 1978 | 647472 |
| transformation of azotobacter vinelandii strains unable to fix nitrogen with rhizobium spp. dna. | the phenotypes of azotobacter vinelandii atcc 12837 strains defective in nitrogen fixation (nif-) were characterized by intrageneric transformation with known nif- strains of a. vinelandii op. these former mutant strains were used as recipients for intergeneric transformation by deoxyribonucleic acid (dna) prepared from rhizobium spp. to determine if the rhizobia would transform the azotobacter nif- phenotypes to nif+. the frequency of nif+ transformants using rhizobium dna was always less than ... | 1978 | 647476 |
| membranes in lupin root nodules. i. the role of golgi bodies in the biogenesis of infection threads and peribacteroid membranes. | the process of infection of lupin nodule cells by rhizobia was examined using thin-section and freeze-fracture electron-microscopic techniques to characterize the properties of different membranes and to establish relationships between them. the membranes of the golgi bodies and the endoplasmic reticulum stained with zinc iodide-osmium tetroxide but not with phosphotungstic acid or silver. by contrast the infection thread membranes, peribacteroid membranes, plasma membranes and membranes of cyto ... | 1978 | 649682 |
| membranes in lupin root nodules. ii. preparation and properties of peribacteroid membranes and bacteroid envelope inner membranes from developing lupin nodules. | peribacteroid membranes and bacteroid envelope inner membranes have been isolated from developing lupin nodules. isolation of the peribacteroid membranes was achieved by first preparing membrane-enclosed bacteroids free from other plant organelles or membranes. the peribacteroid membranes were then released by osmotic shock and purified by centrifugation to equilibrium on sucrose gradients. the bacteroids were broken in a pressure cell and the bacteroid envelope inner membranes were isolated usi ... | 1978 | 649683 |
| intra- and intergeneric similarities of the rrna cistrons of agrobacterium and their significance for the taxonomy of this genus [proceedings]. | 1978 | 655694 | |
| effects of interactions between different culture fractions of 'phosphobacteria' and rhizobium on mycorrhizal infection, growth, and nodulation of medicago sativa. | interactions between cell-free culture supernatants, cells, and the whole cultures of rhizobium and phosphobacteria with endomycorrhizal fungi and their effects on growth and nutrition of medicago sativa grown in a low-phosphate soil were studied. a satisfactory nodulation was greatly dependent on the mycorrhizal symbiosis. cell-free supernatants of rhizobium and phosphobacteria improved plant growth, nodulation and mycorrhiza formation. the activity of phosphobacterial culture seemed to be due ... | 1978 | 657005 |
| [actinomycetes antagonistic to fungi and not affecting rhizobium meliloti]. | the effects of 481 actinomycetes isolated from agricultural soils supporting good growth of alfalfa or clover on two efficient strains of rhizobium meliloti a2 and s14 were studied. strain a2 was inhibited by 28% of the isolates and strain s14 was inhibited by 31% of them. no significant difference was found between the resistance of both actinomycete strains. the effects of the 288 isolates not affecting r. meliloti on six fungi were also studied. the most sensitive fungus was stemphylium sarci ... | 1978 | 657008 |
| transfer from rhizobium japonicum to azotobacter vinelandii of genes required for nodulation. | a mutant strain of azotobacter vinelandii that is unable to fix n2 (nif-) was transformed to nif+ with dna from rhizobium japonicum. of 50 nif+ transformants tested, 3 contained the o antigen-related polysaccharide that is present on the cell surface of a nodulating r. japonicum strain, but is absent from a non-nodulating mutant strain. | 1978 | 659367 |
| [development of nodule bacteria in soil]. | the rate of growth of a population of nodule bacteria in soil and the time it takes for the cell generation depend on the content and accessibility of necessary nutrient substances. the periods of adaptation, active growth, and stabilization of the population number in the course of growth of a population are typical of soddy-podzolic continuous-cultivated soil. the duration of each period is determined by a sum of ecological factors. | 1978 | 661644 |
| influence of light on pectic enzymes in root exudates of trifolium alexandrinum inoculated with rhizobium trifolii. | an in vitro experiment was conduced under bacteriologically controlled conditions to examine the effect of light on the production of pectin methyl esterase (pme) and pectin polygalacturonase (pg) in the root exudates of trifolium alexandrinum inoculated with an efficient strain of rhizobium trifolii. the results revealed that pme and pg increased with an increase in the duration of light to which plants were exposed. however, both the enzymes were detected in the root exudates of nonphotosynthe ... | 1978 | 664934 |
| purification and biochemical properties of complex flagella isolated from rhizobium lupini h13-3. | 1. the complex flagella of rhizobium lupini h13-3 differ from plain bacterial flagella in the fine structure of their filaments dominated by conspicuous helical bands, in their fragility and their resistance against heat decomposition. to elucidate the basis of these differences, the composition of complex filaments and their subunits was analysed. 2. isolated complex flagella containing the filament and hook protions were purified by differential centrifugation. hooks were separated by ultracen ... | 1978 | 667114 |
| nitrogenase--hydrogenase interrelationships in rhizobia. | a review is given of the properties of the hydrogenase present in rhizobium bacteriods together with a discussion and evidence of the function of the enzyme in relationship to nitrogen fixation. the efficiency with which nodules fix nitrogen i.e. the amount of hydrogen evolved as a ratio of the total electron flow through nitrogenase, is considered and the recycling of hydrogen is discussed. attention is drawn to recent work, in which plants which have nodules containing hydrogenase have been sh ... | 1978 | 667179 |
| membrane energization in relation with nitrogen fixation in azotobacter vinelandii and rhizobium leguminosarum bacteroids. | nitrogen fixation in a. vinelandii and r. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the atp/adp-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. although we have been successful in preparing inside-out vesicles which can be energized, attempts to couple these membranes to n2-ase were still unsuccessful. one of the major problems could be the failure to energize these vesi ... | 1978 | 667180 |
| interaction between rhizobium japonicum phage m-1 and its receptor. | the receptor for phage m-1 was present in the exopolysaccharide (eps) of rhizobium japonicum d211. the eps was a heteropolysaccharide consisting of glucose, galactose, glucuronic acid, and glucosamine units. these monosaccharides prevented phage-cell attachment indicating that they may mimick the receptor. phage-cell attachment was either stimulated or inhibited by mg2+ and ca2+ depending upon their concentration. an enzyme which depolymerized the exopolysaccharide releasing oligosaccharides was ... | 1978 | 667736 |
| denitrification in rhizobium. | thirty-three strains of rhizobium were examined for their reduction of nitrate under anoxic conditions. three patterns of dissimilatory nitrate reduction were observed: (1) reduction to n2o and n2 (denitrification), (2) reduction to and subsequent accumulation of no2- (nitrate respiration), (3) no reduction. strains of r. japonicum and the cowpea miscellany displayed all three types, while strains of r. leguminosarum, r. phaseoli, and r. trifolii did not reduce nitrate by dissimilatory means. th ... | 1978 | 667742 |
| involvement of the cytoplasmic membrane in nitrogen fixation by rhizobium leguminosarum bacteroids. | 1. the nitrogen-fixing efficiency of freshly prepared suspensions of rhizobium leguminosarum bacteroids from pea root nodules was considerably enhanced by addition of bovine serum albumin. evidence was found that during preparation of bacteroids the cell membrane is exposed to the uncoupling effect of free fatty acids and to plant phospholipase d activity. both effects could be counteracted by bovine serum albumin. 2. a technique was developed by which concentrations of free o2 and nitrogenase a ... | 1978 | 668685 |
| studies on the mode of action of agrocin 84. | 1978 | 670092 | |
| purification of rhizobium cytochromes p-450. | 1978 | 672626 | |
| fertility inhibition in rhizobium lupini by the resistance plasmid rp4. | r plasmid rp4 inhibits the fertility of r. lupini. an rp4 carrying r. lupini donor strain is no longer capable of transferring chromosomal genes. after loss of rp4 the r. lupini fertility reappears. plasmid rp4 spontaneously mutates at high frequency in r. lupini. rp4 mutants which do not inbibit fertility were isolated. these mutants were always transfer-defective, too. it is postulated that the genetic information for fertility inhibition in r. lupini is a substantial part of the transfer unit ... | 1978 | 672901 |
| influence of ammonium chloride on the nitrogenase activity of nodulated pea plants (pisum sativum). | a study was made on the short-term effect of ammonium ions on the nitrogenase activity of pea root nodules. nodulated pea plants (pisum sativum), having reached maximum acetylene-reducing activity, were supplied with nh4cl (20 mm). nitrogenase activity of intact plants, detached nodules, and isolated bacteroids was measured at differed time intervals. a significant drop (20 to 40%) in the acetylene-reducing activity of treated intact plants and their detached nodules was observed after 1 day. no ... | 1978 | 677873 |
| [demonstration of extrachromosomal dna in rhizobium meliloti]. | seven effective (nitrogen-fixing) strains of rhizobium meliloti have been studied. by sedimentation analysis of their alkaline lysates in alkaline sucrose gradients, a plasmid was found in four strains. in a strain (2011 str 3) which gave no result with this method, supercoiled dna was detected by cscl-dye buoyant density gradient centrifugation. that result was confirmed by analytical cs2so4-ag+ density gradients, which showed a heterogeneity in the average base composition of the dna extracted ... | 1978 | 688104 |
| stimulation of tetrapyrrole formation in rhizobium japonicum by restricted aeration. | cultures of rhizobium japonicum were grown with vigorous aeration to stationary phase and were then incubated under restricted aeration for several days. under these "microaerobic" conditions, cellular heme content increased 10-fold, and visible amounts of porphyrins were released into the culture medium. the two predominant porphyrins produced were identified, on the basis of their spectrophotometric and chromatographic properties, as protoporphyrin and coproporphyrin. the cytochrome complement ... | 1978 | 690074 |
| survival and efficiency of cowpea rhizobia on pelleted and non-pelleted peanut seeds, treated with fungicides. | peanut seeds were either normally inoculated with the legume inoculant okadin, containing cowpea rhizobia, or pelleted and treated with each of the fungicides brassical, thiram, orthocide 75, falisan, vitavax 75, and agrosan. the seeds were then incubated at room (+/- 25 degrees c) or refrigeration temperatures (+/- 5 degrees c). survival tests were made after 2 and 10 days. treated seeds were also planted in pots containing nile silt for testing the efficiency of rhizobia as affected by the fun ... | 1978 | 696041 |
| [identification of actinomycetes with antifungal activity which do not affect rhizobium meliloti]. | thirteen isolates of actinomycetes that have broad antifungal activity and do not affect two efficient strains of rhizobium meliloti were identified as: nocardia autotrophica, streptomyces antimycoticus, s. anulatus, s. capoamus, s. lydicus, s. murinus, s. roseo-luteus, and s. thermotolerans. | 1978 | 709436 |
| coordinated regulation of octopine degradation and conjugative transfer of ti plasmids in agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons. | by using the analog noroctopine, mutants of agrobacterium tumefaciens were isolated with altered regulation patterns for the ti plasmid-borne octopine utilization genes. these could be divided into three classes: (i) strains with a constitutive level of octopine enzymes and a high degree of spontaneous ti transfer; (ii) one strain with constitutive octopine enzymes but no spontaneous ti transfer; and (iii) strains with an altered inducibility in which, contrary to the wild-type ti plasmid, conju ... | 1978 | 711678 |
| protein composition and structure of the sheath and core of a defective bacteriophage from rhizobium. | 1978 | 712879 | |
| [ring structures on the cell wall of agrobacterium tumefaciens (author's transl)]. | 1978 | 713041 | |
| a possible mechanism for the establishment of nitrogen fixation in bacteroids of fast-growing acid-producing rhizobium. | 1978 | 713563 | |
| [nitrogenase activity of rhizobium meliloti and rhizobium vigna in a root tisse culture of leguminous and nonleguminous plants]. | as was shown using the acetylene technique, the nitrogenase activity of rhizobium meliloti and rhizobium vigna increased when they were cultivated with the root tissue cultures of legumes (lucerne, sweetclover) and non-legumes (tobacco, glasswort, carrot), particularly in the case of the former. the maximum activity of nitrogenase was found in r. meliloti. the tissue cultures of legumes had no effect on the growth of rhizobium whereas the tissues of non-legumes stimulated their biomass accumulat ... | 1978 | 713878 |
| carotenoids of rhizobia. ii. the effect of nicotine on the carotenoid pattern of rhizobium lupini. | with increasing concentrations in the growth medium of the cyclization inhibitors nicotine or 2-(4-chlorophenylthio)-triethylamine hydrochloride (cpta) the previously identified bicyclic carotenoids of rhizobium lupini (2,3,2',3'-tetrahydroxy-beta,beta-caroten-4-one and 2,3,2',3'-tetrahydroxy-beta,beta-carotene) were successively replaced by hitherto unknown monocyclic carotenoids. by application of mass and nuclear magnetic resonance spectroscopy 3 carotenoids were identified as 2,3-trans-dihyd ... | 1978 | 718371 |
| adsorption of rhizobia to cereal roots. | 1978 | 718714 | |
| tumor-inducing (ti) plasmids of agrobacterium share extensive regions of dna homology. | labeled ti plasmid dnas from diverse agrobacterium strains were hybridized to southern blots of pti-b6-806 plasmid dna digest fragments of known map order. the map location of dna sequences common to all ti plasmids was found to be extensive, consistent with the view that ti plasmids have evolved from a common ancestral plasmid. | 1978 | 721776 |
| multiple genes coding for octopine-degrading enzymes in agrobacterium. | most biotype 2 strains of agrobacterium tumefaciens and a. radiobacter which utilize nopaline also degrade octopine. in all such strains studied, the ability to degrade octopine did not appear to be transferred to plasmidless recipient cells under conditions of plasmid transfer in which the ability to utilize nopaline was transferred. an octopine-degrading mutant was isolated in a strain cured of its plasmid, suggesting that genes of octopine degradation may have a chromosomal location in some s ... | 1978 | 721779 |
| [competitive capacity of the nodular bacteria of lupine]. | 1978 | 723654 | |
| [proliferative activity of normal and tumorous plant tissues cultivated in vitro]. | proliferative pools of helianthus tuberosus l. explants tissues were studied using autoradiography with 3h-thymidine in the course of normal growth in vitro and tumorous growth induced by agrobacterium tumefaciens. it is determined that on the 3d or 4th day of tumorous transformation the number of cycling cells reaches 30% whereas that of normally growing explants showed 10-11%. no changes were observed in 3h-thymidine transport during this period. | 1978 | 726052 |
| trifluralin effect on pisum--rhizobium relationship. | trifluralin inhibited root and shoot elongation of pisum sativum plant and caused isodiametric increase in cell volume of both tissues. the water content of the plant was not affected. the weedicide inhibited also growth and o2 uptake of rhizobium leguminosarum, isolated from pisum plant. reduction in the nitrogen content of pisum tissues was noticed, and this may be attributed to the inhibition of nodule formation. variations in the free and protein-amino acids were observed in the plant tissue ... | 1978 | 726711 |
| nitrate reductase and dehydrogenase activities of cowpea nodules as affected by ammonium sulphate and urea fertilizers. | 1978 | 726712 | |
| the correlation between the efficiency of rhizobia and nitrate reductase and dehydrogenase activities of cowpea nodules. | cowpea seeds variety fettriat were planted in nile silt soil and inoculated with 5 strains of cowpea rhizobia. after 50 and 80 days, the plants were uprooted, analysed for dry weight, total nitrogen, fresh weight of nodules, nitrate reductase activity in the leaves, and nitrate reductase and dehydrogenase activities in the nodule homogenate in the presence or absence of succinate, citrate, and ethyl alcohol. the data were analysed to establish the correlation coefficients between total nitrogen ... | 1978 | 726713 |
| plasmid-dependent attachment of agrobacterium tumefaciens to plant tissue culture cells. | kinetic, microscopic, and biochemical studies show that virulent ti (tumor inducing)-plasmid-containing strains of agrobacterium attach to normal tobacco and carrot tissue culture cells. kinetic studies showed that virulent strains of a. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. five ti-plasmid-containing virulent agrobacterium strains showed greater attachment to tobacco cells than did ... | 1978 | 730370 |
| nodule infection by bean yellow mosaic virus in phaseolus vulgaris. | infection of root nodules of beans, phaseolus vulgaris l., by bean yellow mosaic virus (bymv) and the effect of the disease on the specific activity of the nodule are reported. infectivity and serological microprecipitin assays with two sources of bymv antiserum demonstrated that nodules from bean plants whose leaves had been inoculated with bymv contain bymv antigen. the disease reduced the fresh weights of tops, roots, and root nodules and induced premature nodule decay and/or nodule drop. the ... | 1978 | 736539 |
| [production of mutants of nodule bacteria which have lost their ability to reduce acetylene]. | 1978 | 745564 | |
| round table discussion: long-range research planning in carbon assimilation. | 1978 | 747609 | |
| rhizobium strain identification in arachis hypogaea nodules by enzyme-linked immunosorbent assay (elisa). | the technique of enzyme-linked immunosorbent assay (elisa) was used for serological identification of peanut rhizobium strains both in cell suspension of pure culture and in single root nodules of groundnut (arachis hypogaea) plants. antisera of three peanut rhizobium strains were tested against eight different rhizobium isolates. three serogroups identified by agglutination and immunodiffusion tests were confirmed by elisa. in this experiment elisa was more sensitive by four to six orders of ma ... | 1978 | 747814 |
| mutagenesis by insertion of the drug resistance transposon tn7 applied to the ti plasmid of agrobacterium tumefaciens. | 1978 | 748948 | |
| fingerprints of agrobacterium ti plasmids. | 1978 | 748949 | |
| restriction endonuclease mapping of a plasmid that confers oncogenicity upon agrobacterium tumefaciens strain b6-806. | 1978 | 748950 | |
| production of adventitious root primordia on hypocotyls of castor bean seedling, infected with agrobacterium tumefaciens. | the development of sterile secondary tumours on hypocotyls of castor bean seedlings, inoculated with any of the ten isolates of agrobacterium tumefaciens tested, were observed below the site of the primary tumours. histopathological studies performed in the present work indicate that the observed secondary tumours were adventitious root primordia and not the ordinary type of secondary tumours. according to the available literature such findings are reported here for the first time. all the teste ... | 1978 | 749411 |
| induction of polygalacturonase production in legume roots as a consequence of extrachromosomal dna carried by rhizobium meliloti. | the ability of rhizobium meliloti to induce polygalacturonase production in legume roots decreased during culture under laboratory conditions but was inducible with mitomycin c. this character was irreversibly lost after treatment with acridine orange. extrachromosomal dna of molecular weight 5.9 x 10(6) daltons was detectable in neutral sucrose gradient but was absent from cells 'cured' with acridine orange. therefore, the ability to induce the enzyme production may be controlled by a plasmid. | 1978 | 750886 |
| ability of free-living nodule bacteria to fix atmospheric nitrogen. | literature material is presented on the ability of free-living nodule bacteria for asymbiotic nitrogen fixation, detected for the first time in 1975. necessary components of the nutrient medium in the use of which the nitrogen-fixing ability of rhizobia in pure cultures is manifested, proved to be sugars and intermediates of the citric acid cycle, as well as small quantities of bound nitrogen. the experimental data available in the literature are evidence of the presence of a complete assortment ... | 1978 | 751688 |
| quantitative cytochemistry of glycogen in cells of pea and lupine nodule bacteria. | using a fluorescent variation of the method of pas reaction with auramine oo-so2, the content of pas-positive substance, identified on the basis of supplementary treatments of the preparations with alpha-amylase and a hot mixture of chloroform with methanol and glycogen, was determined cytofluorimetrically in cells of effective and ineffective strains of lupine and pea nodule bacteria in pure culture and under conditions of symbiosis. the largest level of glycogen was detected in bacteroid forms ... | 1978 | 751689 |
| electron microscopic study of the rhizosphere in situ. | 1978 | 751698 | |
| inhibitory effects of the herbicide trifluralin on the establishment of the clover root nodule symbiosis. | trifluralin, a widely used herbicide (rahman, 1977), may act via interaction with plant microtubule protein (hess and bayer, 1974, 1977). in trifluralin treated seedlings of trifolium repens, root tips increased in diameter and decreased in length, cell elongation was decreased, cell wall deposition was abnormal, root hairs were deformed, and a marked reduction was seen in the number of infection threads induced by the bacterial symbionts. our observations support the hypothesis that trifluralin ... | 1978 | 751777 |
| adaptability of rhizobium meliloti in callus tissues of alfalfa roots. | the establishment of associative interrelations between nodule bacteria and cells of a callus culture of alfalfa roots, evaluated according to the criterion of nitrogenase activity, was accompanied by considerable inhibition of the plant cells in the period of maximum infection as well as by the dispersal of actively multiplying nodule bacteria on the surface of the callus cells in the first 7 days after infection. the dynamics of the localization of nodule bacteria on the surface of callus cell ... | 1978 | 754813 |
| action of metabolites of isolated plant tissues on the nitrogenase activity of rhizobium vigna and rhizobium meliloti. | the dependence of the nitrogenase activity of rhizobium meliloti on the strain peculiarities of the cultures, the composition of the media used, and the metabolites of legume tissue cultures was demonstrated by the acetylene method. the nitrogenase activity is significantly higher in r. vigna than in r. meliloti, under the same experimental conditions. enrichment of the murashige-skoog medium with arabinose (25 mm), succinate (25 mm), glutamine (2 mm nitrogen), and yeast extract (0.1%) substanti ... | 1978 | 754814 |
| properties of the hydrogenase system in rhizobium japonicum bacteroids. | 1978 | 564190 | |
| [production of inoculants leguminoses]. | 1978 | 288116 | |
| molecular cloning as a tool to the analysis of the ti plasmids of agrobacterium tumefaciens [proceedings]. | 1978 | 289306 | |
| proteins conferred by the virulence-specifying plasmid of agrobacterium tumefaciens c-58. | membrane-associated and periplasmic proteins of agrobacterium tumefaciens c-58 were compared with those from avirulent (nontumrigenic) derivative strains by slab and two-dimensional gel electrophoresis. two proteins (per-i and per-2), with a molecular weight of 37,500 and 37,300, respectively, were detected in the supernatant fraction of cells of strain c-58 treated with edta and lysozyme in which a 117-megadalton plasmid confers virulence on the organism. the same proteins are missing in an av ... | 1978 | 278990 |
| transfer of nitrogen fixation genes from a bacterium with the characteristics of both rhizobium and agrobacterium. | strain t1k, reported to be rhizobium trifolii strain t1 carrying the drug resistance plasmid ru-1drd, was able to transfer a cluster of nif+ genes to escherichia coli k-12. additional genetic material, resembling the gal-chla region of e. coli, was also transferred from strain t1k. the segregation pattern of these transferred genes suggested that they were on a plasmid. although strain tik was able to nodulate red and white clover, it also formed very slow-growing galls on tomato stems and share ... | 1978 | 342496 |
| incorporation and maintenance of recombinant-dna plasmid vehicles pbr313 and pcr1 in plant protoplasts. | 1978 | 344069 | |
| transfection and transformation of agrobacterium tumefaciens. | the freeze thaw transfection procedure of dityatkin et al. (1972) was adapted for the transfection and transformation of a. tumefaciens. transfection of the strains b6s3 and b6-6 with dna of the temperate phage ps8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. in transformation of the strain gv3100 with the p type plasmid rp4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. agrobacterium ti-plasmids were int ... | 1978 | 355847 |
| [the classification of agrobacterium and rhizobium phages (author's transl)]. | all phages studied are tailed, belong to six morphological groups, and are classified by morphological and serological properties and by physico-chemical parameters of the virion and its nucleic acid. four species of agrobacterium and 12 species of rhizobium phages are described, and their relationship with enterobacteria phages discussed. they include 68 viruses, whereas 60 poorly known phages have not been classified. | 1978 | 370746 |
| transfer of rp4::mu plasmids to agrobacterium tumefaciens. | 1978 | 372975 | |
| spontaneous formation of cointegrates of the oncogenic ti-plasmid and the wide-host-range p-plasmid rp4. | 1978 | 372976 | |
| some aspects of fermentation of rhizobium leguminosarum with reference to economy of ingredients in the substrate. | incremental feeding of nutrients was found to be beneficial in mass cultivation of r. leguminosarum. similarly, semi-continuous fermentation of r. leguminosarum up to 4 days was also found to be useful in maintaining the viable number of cells. | 1978 | 373307 |
| involvement of rhizobium japonicum o antigen in soybean nodulation. | non-nodulating mutant strains of rhizobium japonicum lacked a surface antigen that was present on the wild type. this surface antigen is associated with the o antigen portion of the lipopolysaccharide. paper chromatography of hydrolyzed lipopolysaccharide and o antigen revealed three major component differences between the non-nodulating strains and the wild type. | 1978 | 565351 |
| ultrastructure of rhizobium japonicum in relation to its attachment to root hairs. | in rhizobium japonicum strain nitragin 61a76, morphologically distinct types of bacteria were found to occur in yeast extract-mannitol broth cultures, at both mid-log and stationary phases. of these only the capsular form, characterized by a smooth cell envelope, storage granules (glycogen and poly-beta-hydroxybutyric acid), and an amorphous extracellular capsule, bound soybean lectin. the binding site was localized in the capsular material. less than 1% of the bacterial population differentiate ... | 1978 | 565352 |
| hydrogen evolution and uptake by nodules of soybeans inoculated with different strains of rhizobium japonicum. | hydrogen evolved by nitrogenase may be recycled by a hydrogenase present in some legume nodules. anoka and portage cultivars of soybeans were inoculated with each of 8 and 24 strains, respectively, of rhizobium japonicum and surveyed for h2 evolution and c2h2 reduction rates nodule weight, and plant dry weight. six of the strains (3ilb 110, usda 122, usda 136, 3ilb 6, 3ilb 142, and 3ilb 143) which exhibited no h2 evolution in air were shown to take up h2. the relative efficiencies of nitrogenase ... | 1978 | 565672 |
| role of lectins in plant--microorganism interactions. iv. ultrastructural localization of soybean lectin binding sites of rhizobium japonicum. | the binding of purified, ferritin-labeled soybean seed lectin to the cell surfaces of rhizobium japonicum 31 lb 138 has been examined by whole mount, thin section, and freeze-etch electron microscopy. the ferritin-labeled lectin binds in a biochemically specific manner to the capsular material of this bacterium. the lectin does not bind to the outer membranes of the cells or to flagella. labeled lectin binds to sites throughout the capsular structure, although the density of labeling is somewha ... | 1978 | 567091 |
| [determination of the nitrogen-fixing activity of rhizobium japonicum under sterile microvegetative conditions]. | the nitrogen fixing activity of rhizobium japonicum in symbiosis with soya grown in the sterile microvegetative conditions at an air humidity of 70%, at a temperature of 20 degrees c and a length of light day of 16 hours was assayed using the acetylene technique. the plants were cultivated in phytotron in glass tubes (245 cm3) illuminated with xenon lamps. this technique can be used, apparently, to determine the nitrogen fixing activity of other legumes and cereals. | 1978 | 568711 |
| preparation of a complementary dna for leghaemoglobin and direct demonstration that leghaemoglobin is encoded by the soybean genome. | in soybean root nodules, leghaemoglobin (lb) accounts for 25--30% of the total soluble protein but is not detected in other tissues. in order to determine whether the lb genes are plant or bacterial in origin a cdna probe for lb was prepared from 9s poly (a) containing mrna of root nodules. although this 9s mrna directed synthesis of predominantly three forms of lb in vitro, the kinetics of hybridisation of cdna and the 9s mrna showed a transition at about 30% hybridisation which suggested that ... | 1978 | 569292 |
| efficiency of rhizobium japonicum strains as affecting nitrate reductase and dehydrogenase activities of the soybean nodules. | 1978 | 569408 | |
| genetically marked rhizobium identifiable as inoculum strain in nodules of soybean plants grown in fields populated with rhizobium japonicum. | the fate of an inoculum strain of rhizobium japonicum was studied using a genetically marked strain i-11o subline carrying resistance markers for azide, rifampin, and streptomycin (i-110 ars). at the time of planting into a field populated with r. japonicum, seeds of soybean cultivars kent and peking were inoculated with varying cell densities of strain i-110 ars. at various times during the growing season, surface-sterilized root nodules were examined for the presence of the inoculum strain by ... | 1978 | 570015 |
| bacteriocin-like substances produced by rhizobium japonicum and other slow-growing rhizobia. | bacteriocin-like substances were commonly produced by slow-growing rhizobium japonicum and cowpea rhizobia on an l-arabinose medium. antagonism between strains of r. japonicum was not detected in vitro; however, such strains were often sensitive to some bacteriocins produced by cowpea rhizobia. inhibitory zones (2 to 8 mm from colony margins), produced by 58 of 66 r. japonicum test strains, were reproducibly detected with corynebacterium nebraskense as an indicator. quantitative production was n ... | 1978 | 570016 |
| quality and rate of extracellular polysaccharides produced by rhizobium meliloti and their inducing effect on polygalacturonase production in legume roots as derived from the presence of extrachromosomal dna. | the ability of extracellular polysaccharides of different strains of rhizobium meliloti to induce the production of polygalacturonase by roots of medicago sativa seedlings has been studied. some strains showed a high inducing activity while those derived from them, after treatment with acridine orange and in which extrachromosomal dna was absent, did not show this character. a comparative study of polysaccharide production and preliminary studies on the chemical composition of the active fractio ... | 1978 | 755154 |
| effect of sublethal heat injury on tumour induction and rna synthesis in agrobacterium tumefaciens. | when cells of agrobacterium tumefaciens are subjected to sublethal heat injury at 45 degrees c for 20 min, less than 5% of the viable population retain their ability to initiate tumour formation on kalanchöe daigremontiana. if the cells are then incubated in phosphate buffer at 27 degrees c for 2 h, tumour initiation returns to control levels. inhibitors of dna and protein synthesis had little effect on the recovery of tumour initiation after heat injury. rifamycin, a specific inhibitor of rna s ... | 1978 | 756950 |
| quality control of commercial inoculants for legumes in uruguay (1963-1975). | 1978 | 290009 | |
| varieties of mutualistic interaction in population models. | 1978 | 732344 | |
| studies on bacteroid size and nucleic acid content of alfalfa bacteroids fractionated by velocity sedimentation. | a velocity sedimentation procedure was described to fractionate bacteroids of alfalfa nodules into four subpopulations. bacteroids in these subpopulations were different in size and nucleic acid content as determined by microscopy and flow-microfluorometry (fmf). the slowest-sedimenting bacteroids (fraction i) were small and resembled free-living rhizobium meliloti both in size and nucleic acid content. the fastest-sedimenting bacteroids (fraction iv) were 2 to 3 times longer and contained 3 to ... | 1978 | 728854 |
| electron microscopy and computerized evaluation of some partially denatured group p resistance plasmids. | dna of the r plasmids rp1, rp4 and rp8 was isolated from various hosts. the lengths of these plasmid molecules were determined by electron microscopy: rp1 and rp4 were about 19 micron long, rp8 measured 31 micron. an rp4 plasmid mutant, designated rp4a, was isolated from escherichai coli; it was about 1 micron shorter than normal rp4 dna. to investigate the molecular relationship between rp4, rp4a and rp8 dnas of these plasmids were partially denatured and examined in the electron microscope. me ... | 1978 | 416173 |