Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [growth of nodule bacteria in soil]. | as was found with the aid of capillary microscopy, the nodule bacteria of pea and lupine in soil are encountered as rods, bacteroids, and cocci. the rod-like cells form bacteroids not only in the nodules but also outside, in soil. the bacteroids are viable and later reproduce the coccoid cells (arthrospores). the rods and bacteroids prevail in soil during flowering of the legumes, and the cocci are predominant at the end of vegetation. | 1975 | 1226146 |
| studies on certain factors influencing legume-rhizobia interrelationship. iii. antagonistic soil streptomycetes in the rhizosphere region. | 1975 | 1233355 | |
| leghaemoglobin and the supply of o2 to nitrogen-fixing root nodule bacteroids: presence of two oxidase systems and atp production at low free o2 concentration. | studies of rates of consumption of dissolved o2 by suspensions of bacteroids (rhizobium japonicum, strain cb1809) from soybean root nodules showed the presence of two different terminal oxidase systems. a high-affinity system, sensitive to inhibition by n-phenylimidazole and by carbon monoxide, was most active when the dissolved o2 was between 0-01 and 0-1 mum. at 1 mum-o2 or higher, this oxidase system had little activity and o2 was consumed largely by a low-affinity system insensitive to these ... | 1975 | 1239489 |
| metabolism of p-hydroxybenzoic acid by certain strains of rhizobia. | 1975 | 1242251 | |
| effect of seed fumigation on the symbiosis of rhizobium sp. with arachis hypogaea linn. | 1975 | 1242253 | |
| efficiency of rhizobium leguminosarum as affected by certain herbicides and nematocides. | 1975 | 1243844 | |
| on the relationship among weight of nodules, their leghaemoglobin and bacteroid content, dry weight, and nitrogen content of soybean. | 1975 | 1243845 | |
| [antagonism of pozol and agrobacterium azotophilum on various species of bacteria and fungi, some of them pathogenic to man]. | 1975 | 1059217 | |
| [nitrogen fixation in monospecific and mixed cultures of aerobacter aerogenes and agrobacterium azotophilum using different sources of carbon]. | 1975 | 1059219 | |
| improvements of the membrane filter method for dna:rrna hybridization. | we describe and recommend the following improvements of dna:rrna membrane filter hybridization methods. one of our aims was to avoid dna release from filter discs during hybridization. 1. our hybridization conditions are 2 ssc in aq. dest., with 20% formamide, 50 c, overnight for 16 hr. 2. duplexing is over in 8-10 hr. 3. formamide has to be very pure (o.d. less than or equal to 0.2/cm light path at 270 nm). 4. rnaase treatment: 250 mug/5 ml 2 ssc/filter at 37 c for 1 hr. 5. our conditions for s ... | 1975 | 1082296 |
| comparison of dna polymerase of rhizobium meliloti and alfalfa bacteroids. | dna dependent-dna polymerase activity was established and partially purified from extracts of cultured rhizobium meliloti, f-28, and nodule bacteroids (r. meliloti, f-28) of alfalfa plants (medicago sativa). polymerase activity in the partially purified fractions showed characteristic dependence on mg(2+), dna, and a full complement of deoxyribonucleoside triphosphates. dnase activity, preference of "activated" double strand dna, and inhibition by p-chloromercuribenzoate and mncl(2) were respons ... | 1975 | 16659337 |
| attachment of a long-tailed rhizobium bacteriophage to the pili of its host. | bacterial strain 16-12 was isolated from the root nodules of lupines and was found to be mitomycin c-inducible for the production of a bacteriophage ("16-12-1") with a long noncontractile tail. the phage was found to attach with a fork-like terminal tail structure to the pili of strain 16-12. in addition, it was also found adsorbed to the bacterial cell poles. it is suggested that phage 16-12-1 may be pilus dependent. | 1975 | 16789164 |
| neutral lipids and phospholipids of free-living and bacteroid forms of two strains of rhizobium infective on lotus pedunculatus. | the neutral lipids and phospholipids of two strains of rhizobia in their free-living state and in symbiosis with a host plant are described. the principal lipid classes found were the polymer poly-beta-hydroxybutyrate, phospholipids, free fatty acids, glycerides, methyl esters, aliphatic alcohols, and hydrocarbons. the lipids include unusual unsaturated methyl-branched and saturated methoxy-branched fatty acids. most components were found to be common to both forms of both strains, although the ... | 1975 | 16350025 |
| relation between glutamine synthetase and nitrogenase activities in the symbiotic association between rhizobium japonicum and glycine max. | the activity and extent of adenylylation of glutamine synthetase was examined in both free-living and bacteroid forms of rhizobium japonicum in the presence of excess ammonia. ammonia caused an apparent repression of glutamine synthetase in free-living r. japonicum and adenylylation of the enzyme was also increased. in contrast, neither the activity nor the extent of adenylylation of the bacteroid enzyme was consistently affected by ammonium treatment of bacteroid suspensions. similar results we ... | 1976 | 16659522 |
| synergetic cultures of glycine max root cells and rhizobia separated by membrane filters. | when suspension cultures of actively growing soybean (glycine max l.) root cells were separated by two or three membrane filters from suspension cultures of the bacteria, a synergetic (cooperative) activation of nitrogenase was observed in the rhizobium japonicum used in the bacterial side. either plant cells or plant cell-conditioned medium was needed for this activation to take place. both acetylene reduction and hydrogen evolution by the activated r. japonicum persisted for several days after ... | 1976 | 16659545 |
| chemotaxis of rhizobium spp. to plant root exudates. | rhizobium spp. show chemotaxis to plant root exudates. both legumes and non-legume root exudates attract the different rhizobia studied. however, the bacteria show a differential response in that they are attracted to the root exudates of some plants and show no attraction toward others. an example of negative chemotaxis was also observed. the trefoil strain of rhizobium shows chemotaxis which is qualitatively different from that observed in other bacteria in that simple sugars, di-and trisaccha ... | 1976 | 16659577 |
| effect of protein additives on acetylene reduction (nitrogen fixation) by rhizobium in the presence and absence of soybean cells. | the effect of protein additives on acetylene reduction (n(2) fixation) by rhizobium associated with soybean cells (glycine max [l.] merr.) in vitro was studied. acetylene reduction was promoted on the basal medium supplemented with 1.4 mg of n/ml supplied as aqueous extracts of hexane-extracted soybean, red kidney beans (phaseolus vulgaris l.), or peas (pisum sativum l.). commercial samples of alpha-casein, or bovine serum albumin also promoted acetylene reduction at a concentration of 1.4 mg of ... | 1976 | 16659592 |
| l-sorbose metabolism in agrobacterium tumefaciens. | the pathway of l-sorbose metabolism in agrobacterium tumefaciens strain b6 was determined to be: l-sorbose leads to d-glucitol (sorbitol) leads to d-fructose leads to d-fructose-6-phosphate leads to d-glucose-6-phosphate. the reduction of l-sorbose and the oxidation of d-glucitol were mediated by nadph- and nad+-linked oxidoreductases, respectively. the intermediates, d-glucitol and d-fructose, were isolated from in vitro reaction mixtures by column chromatography on dowex 1-borate, and identifi ... | 1976 | 1085123 |
| construction of a p plasmid carrying nitrogen fixation genes from klebsiella pneumoniae. | 1976 | 768778 | |
| the molybdenum--iron protein of klebsiella pneumoniae nitrogenase. evidence for non-identical subunits from peptide 'mapping'. | the molybdenum- and iron-containing protein components of nitrogenase purified from klebsiella pneumoniae, azotobacter vinelandii, azotobacter chroococcum and rhizobium japonicum bacteroids all gave either one or two protein-staining bands after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, depending on the commercial brand of sodium dodecyl sulphate used. the single band obtained with k. pneumoniae mo-fe protein when some commercial brands of sodium dodecyl sulphate were used in t ... | 1976 | 779772 |
| conjugation in agrobacterium tumefaciens in the absence of plant tissue. | a general, reliable conjugation system for agrobacterium tumefaciens in the absence of plant tissue is described in which a. tumefaciens can serve either as the donor or recipient of plasmid deoxyribonucleic acid with reasonable efficiency. plasmid rp4 was transferred from escherichia coli to a. tumefaciens and from strain of a. tumefaciens. both rp4 and the a. tumefaciens virulence-associated plasmids were detected by alkaline sucrose gradients in a. tumefaciens strains a6 and c58 after mating ... | 1976 | 783141 |
| properties of plasmids constructed by the in vitro insertion of dna from rhizobium leguminosarum or proteus mirabilis into rp4. | plasmids have been constructed by insertion of dna from rhizobium leguminosarum or proteus mirabilis into rp4 (an r factor of group p). such recombinant plasmids retain the wide host range of the parental plasmid, being as efficiently transmissible as the unmodified rp4 and are stably maintained in rapidly growing cultures. the recombinant plasmids, even though each contained a dna sequence absolutely identical with that of the host strain, are no more efficient at mobilizing the transfer of chr ... | 1976 | 787766 |
| inhibitory effect of streptomyces antibioticus and other microorganisms on rhizobium. | 1976 | 789197 | |
| [selection methods of nodule bacteria]. | the aim of selecting nodule bacteria is to obtain stable and genetically pure strains with elevated effectiveness in the conditions of symbiosis with bean plants, sufficient viability and competing ability, which would survive in soil for a long time. the following methods have been suggested to solve this task: selection by plants using mutagenic factors; genetic markers; production of genetically homogeneous material; determination of the degree of persistance effectiveness in mutants. | 1976 | 794638 |
| [molecular nitrogen fixation by pure cultures of nodular bacteria (a literature review)]. | 1976 | 800021 | |
| oxygen requirement for acetylene reduction by pure cultures of rhizobia. | the oxygen and nutritional requirements for acetylene reduction by rhizobium japonicum and rhizobium sp. in liquid culture are described. the optimal oxygen concentration was about 0.1% in the gas phase, which is lower than that of any other known aerobic nitrogen-fixing microorganism. these organisms are also unique in that nitrogenase synthesis is not repressed in the presence of ammonium chloride under certain cultural conditions, in contrast to other wild-type bacteria. | 1976 | 931945 |
| octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of agrobacterium tumefaciens. | several nopaline degrading strains and one octopine degrading strain are shown to loose oncogenicity as well as the ability to utilize these guanidine compounds when they are cured of their ti plasmid. to investigate whether the specific genes involved in the utilization of one or the other compound are located on the plasmid, plasmid-transfer experiments have been performed. the plasmid from a nopaline degrading strain has been transferred to a naturally non oncogenic agrobacterium namely a. ra ... | 1976 | 934053 |
| occurrence of trans-ribosylzeatin in agrobacterium tumefaciens trna. | 1976 | 934334 | |
| mixed inoculations with effective and ineffective strains of rhizobium leguminosarum. | 1976 | 939736 | |
| [the effect of nitrate and nitrite on the respiration and cytochrome system of rhizobium lupini]. | differential spectrophotometry and oxygen electrode were used to study the effect of nitrate and nitrite on the cytochrome system and respiration of rhizobium lupini cultivated in the conditions of different aeration, and bacteroids of this strain isolated from lupine nodules. in the anaerobic conditions, nitrate (10(-3) m) accepts electrons from the cytochrome system in suspensions of bacteroids and bacterial cells grown in the microaerophilic conditions (12 mum o2), but does not oxidize cytoch ... | 1976 | 940501 |
| host-symbiont interactions. i. the lectins of legumes interact with the o-antigen-containing lipopolysaccharides of their symbiont rhizobia. | 1976 | 945740 | |
| a study on the effect of certain phenolic acids and fumarie acid in soil on the development of paddy seedlings and nitrogen-fixing bacteria. | 1976 | 947114 | |
| studies on seed coat toxicity to rhizobia of urid (phaseolus mungo), mung (phaseolus aureus), and soybean (glycine max). | 1976 | 947116 | |
| resistance to antibiotics in rhizobium trifolii and its relation to nitrogen fixation. | 1976 | 947117 | |
| [separation into components and recombination of nitrogenase from lupine bacteroids]. | 1976 | 947717 | |
| nitrogenase activity and respiration of cultures of rhizobium spp. with special reference to concentrations of dissolved oxygen. | studies of nitrogenase in cultures of the cowpea rhizobia (rhizobium spp.) strains 32h1 and cb756 are reported. preliminary experiments established that, even when agar cultures were grown in air, suspensions of bacteria prepared anaerobically from them were most active at low concentrations of free dissolved o2. consequently, assays for activity used low concentrations of o2, stabilized by adding the nodule pigment leghaemoglobin. in continuous, glutamine-limited cultures of 32h1, nitrogenase a ... | 1976 | 953022 |
| the presence of agrobacterium tumefaciens plasmid dna in crown gall tumour cells. | 1976 | 956781 | |
| reduction of acetylene by stationary cultures of free-living rhizobium sp. under atmospheric oxygen levels. | the reduction of acetylene to ethylene by stationary (non-shaking) cultures of free-living rhizobia under atmospheric oxygen levels has been demonstrated. under these conditions the development of the activity is inhibited by 10 mm nh4cl and about 20% of oxygen is required for maximal activity. when the stationary cultures were shaken, oxygen concentrations of 1% and higher were found to be inhibitory. specific activities of 20 and 40 nmol of acetylene reduced h-1 mg-1 protein were observed. | 1976 | 963617 |
| monomethyl sugars in extracellular polysaccharides from slow-growing rhizobia. | 1976 | 963704 | |
| adsorption of bacteria to roots as related to host specificity in the rhizobium-clover symbiosis. | quantitative microscope techniques were utilized to examine the adsorption of rhizobial cells to clover root hairs. adsorption of cells of noninfective strains of rhizobium trifolii or infective r. meliloti strains to clover root hairs was four to five times less than that of the infective r. trifolii strains. attachment of the rod-shaped bacteria to clover root cells occurred in a polar, end-on fashion. viable or heat-killed r. trifolii cells precoated with a clover lectin having 2-deoxyglucose ... | 1976 | 970936 |
| rp4 promotion of transfer of a large agrobacterium plasmid which confers virulence. | introduction of rp4 plasmid into agrobacterium tumefaciens promotes the transfer on solid medium of large virulence-associated plasmids from virulent donor strains to a plasmidless avirulent recipient. exconjugants were selected for the ability to utilize octopine or nopaline as the sole source of arginine, traits which are coded for by virulence-associated plasmids in the strains employed here. all exconjugants retained the arginine auxotrophy of the recipient strain, and were resistant to ampi ... | 1976 | 971805 |
| physical characteristics of dna from bacteriophages of agrobacterium tumefaciens. | dna was extracted from isolates of bacteriophages grown on virulent and avirulent strains of agrobacterium tumefaciens. molecular weights of dna from phages isolated from the virulent a. tumefaciens (iibv7) were about 41.5 x 10(6) daltons, while those from the avirulent a. tumefaciens (iibnv6) were about 32.5 x 10(6) daltons. the buoyant densities of the four dna's ranged from 1.7086 to 1.7089 g/cm3, values that were not significantly different. dna-dna hybridization studies also indicated that ... | 1976 | 974908 |
| isoenzymes of acid phosphatase and non-specific esterases in cultures of neoplastic and normal tobacco tissues. | axenic cultures of normal, habituated and crown gall teratoma were grown under varying conditions to examine the effects of environment on the expression of neoplastic character. acid phosphatase patterns on polyacrylamide gels did not vary greatly among tissues although there were differences in acid phosphatase activity between various strains of agrobacterium tumefaciens, the bacteria which cause crown gall. certain esterase isoenzymes were found only in tissues grown on specific media, while ... | 1976 | 976652 |
| properties of the cured oncogenic strain 37400 of agrobacterium tumefaciens. | the properties of the 37400 oncogenic strain of agrobacterium tumefaciens are described. this strain was derived from the vi lysogenic strain originally isolated by hamilton from a zinnia elegans tumour. strain 37400 has a number of properties which render it suitable for quantitative and genetic studies. it is cured of prophages and can serve as a universal sensitive indicator for a number of phages isolated from various lysogenic strains of agrobacterium tumefaciens. its good growth properties ... | 1976 | 976880 |
| isolation and complementation analysis of temperature-sensitive mutants of the ps8 bacteriophage of agrobacterium tumefaciens. | 1976 | 976881 | |
| plasmid deoxyribonucleic acid in rhizobium trifolii. | in deoxyribonucleic acid of rhizobium trifolii centrifuged in cesium chloride-ethidium bromide equilibrium was found a sattelite peak containing covalently closed circular deoxyribonucleic acid. the plasmid had a molecular weight of about 64 x 10(6) shown by sedimentation in sucrose gradients and electron microscopy. | 1976 | 977542 |
| isolation and characterization of agrobacterium tumefaciens mutants affected in the utilization of octopine, octopinic acid and lysopine. | using an enrichment procedure, mutant strains of agrobacterium tumefaciens were isolated that lacked the ability to utilize octopine as a nitrogen source. of 55 such isolates, 44 were unable to utilize several amino acids; the remaining 11 strains were altered solely in their ability to utilize octopine, octopinic acid and lysopine. it is concluded that only the latter were plasmid mutations. among them, there was a high, but no absolute, correlation with avirulence. all strains contained the t1 ... | 1976 | 978177 |
| the origin of endosymbiotic nitrogen fixation by rhizobium. | 1976 | 979293 | |
| [chemistry of denitrification in sporogenous soil bacteria]. | soil sporeforming denitrifying bacteria, bacillus filaris and bacillus polymyxa, differ by their cultural-morphological and physiological characteristics, but are similar in the chemistry of dissimilating nitrate reduction. two processes occur simultaneously: denitrification yielding gaseous nitrogen forms, and nitrate respiration upon which nitrates are reduced to ammonia. the ratio between the two depends on physico-chemical conditions of the environment. the chemistry of dissimilating nitrate ... | 1976 | 979677 |
| [relationship between crown-gall plant tumors and the cell cycle]. | it is established that under conditions of the culture in vitro the greatest amount of crown-gall swellings on topinambur and carrot explants is formed with agrobacterium tumefaciens inoculation 4 and 6 hrs after the tissue extraction and planting, which corresponds to the g1-phase of the 1st cellular cycle. in the inoculated tissue cells entrance to the s-phase is accelerated and the maximal number of labelled nuclei is found 10 hrs earlier. | 1976 | 982581 |
| chemical nature of agrocin 84 and its effect on a virulent strain of agrobacterium tumefaciens. | agrocin 84, produced by agrobacterium radiobacter k84, inhibited ribonucleic acid, deoxyribonucleic acid, and protein synthesis and amino acid transport in a susceptible, virulent strain of a. tumefaciens h-38-9. cell motility was immediately stopped by action of the agrocin, 50% of the cells were killed within 15 min of contact, and the remainder were inhibited. agrocin 84 is trypsin and pepsin resistant, but chemical analysis indicated a small peptide with a molecular weight of 2,500 containin ... | 1976 | 984792 |
| regulation of nitrogen fixation in rhizobium sp. | regulation of nitrogen fixation by ammonium and glutamate was examined in rhizobium sp. 32h1 growing in defined liquid media. whereas nitrogenase synthesis in klebsiella pneunoniae is normally completely repressed during growth on nh4+, nitrogenase activity was detected in cultures of rhizobium sp. grown with excess nh4+. however, an "ammonium effect" on activity was invariably observed in cultures grown on nh4+ as sole nitrogen source; the nitrogenase activity was, depending on conditions, 14 t ... | 1976 | 984826 |
| ineffective and non-nodulating mutant strains of rhizobium japonicum. | mutant strains of rhizobium japonicum that were unable to allow the corsoy cultivar of soybean to reduce acetylene or fix n2 were isolated. these strains grow as well as the wild type in a variety of media. mutant strains sm1 and sm2 did not form nodules on the host plant; however, they reduced acetylene in the nonsymbiotic assay. strains sm3 and sm4 produced nodules that did not have the characteristic pink pigment caused by leghemoglobin. the nodules formed by these strains also were small. on ... | 1976 | 986388 |
| rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization. | four derivatives of rhizobium japonicum 110 were isolated on the basis of morphologically different colonies formed on yeast extract-mannitol-hm salts medium. all are able to nodulate lee soybeans. the bacteria-plant associations formed by each clone have measurable acetylene-reducing activity, but those formed by two of these clones (designated l1-110 and l2-110) are 5- to 10-fold less efficient than those formed by the others (designated i-110 and s-110). these derivatives were not detectable ... | 1976 | 988784 |
| effectiveness of rhizobia from wild species of arachis on the cultivated species, a. hypogaea and their physiological characteristics. | 1976 | 989215 | |
| root exudation in relation to innoculation with rhizobia. | 1976 | 989216 | |
| biochemical changes in the roots of legumes upon inoculation. | 1976 | 989217 | |
| nitrogen fixation by microbial cultures with sodium salt of organic acids as carbon source. | the best source of carbon, when used as the sodium salt of organic acids, is sodium salicylate which shows highest nitrogen fixation and also appreciably large amounts of nitrogen fixed per g carbon consumed. next in order is sodium benzoate, then oxalate. sodium citrate is followed by sowium acetate in the order of decreasing efficiency. | 1976 | 989656 |
| nitrate reductase from anaerobically grown rhizobium japonicum. | the activity of nitrate reductase in rhizobium japonicum is controlled by oxygen tension, and not by nitrate. the enzyme from r. japonicum grown anaerobically in the presence of nitrate resembles that from bacteroids in having a molecular weight of about 69000 daltons; the enzyme from aerobically grown cells ahs a molecular weight of about 170000 daltons. both types of enzyme have similar km values, but differ in their sensitivity to kcn. | 1976 | 993777 |
| [the presence of agrobacterium tumefaciens in lucerne root nodules]. | agrobacterium tumefaciens does not penetrate into nodules on the roots of lucerne with the active strain of nodule bacterium as was established with the aid of genetic markers and plant selection. a nodule, whose shape was not typical and which did not fix nitrogen, was formed on the root of lucerne inoculated with the culture of agrobacterium tumefaciens treated with uv. a bacterial strain isolated from the nodule was identical to a. tumefaciens according to its resistance to streptomycin and s ... | 1976 | 1004260 |
| [transformation as a method for increasing the activity of lucerne nodule bacteria]. | transformation of lucerne nodule bacteria was tried as a method for increasing their activity. about 100 strains resistant to 1000 units/ml of streptomycin have been obtained by transformation; 34 strains were tested for the activity of nitrogen fixation in conditions of a microvegetative experiment according to the scheme: strain-donor, strain-recipient, transformed strains. the activity varied within a wide range: +/-30--40% cf. the activity of the parent strains. the transformed strains were ... | 1976 | 1004274 |
| [molecular nitrogen fixation by rhizobium in association with tobacco and wheat cells]. | 1976 | 1009823 | |
| [lipopolysaccharides of rhizobium lupini in its symbiotic form]. | 1976 | 1009837 | |
| characterization of soluble and structural proteins of rhizobium cells and bacteroids from root nodules. | 1976 | 1010553 | |
| characterization of ribosomal rna & proteins from rhizobium cells, bacteroids & phaseolus aureus roots. | 1976 | 1010576 | |
| [role of leguminous plants in effective symniosis with nodule bacteria]. | experiments were conducted with five clover varieties and six lucerne varieties infected with their specific strains of nodule bacteria with a high or low activity. the role of plants in active symbiosis with nodule bacteria was established. the nitrogen fixing activity of nodule bacteria varied within a wide range depending on the plant variety. not only the activity of the strain, but the variety of the plant too, should be taken into account for industrial purposes. | 1976 | 1012047 |
| [survival of rhizobium meliloti in soil studied by genetic labeling]. | the number of cells of a mutant of rhizobium meliloti resistant to streptomycin decreased to 10(6) per 1 g of soil during 3-5 days and remained at this level for at least a month when 10(8) cells of the mutant per 1 g were introduced into non-sterile soil. factors causing such a stability of the population are discussed. | 1976 | 1012051 |
| isolation of 3-o-methyl-d-ribose from a rhizobium polysaccharide. | 1976 | 1016992 | |
| trans-zeatin in culture filtrates of agrobacterium tumefaciens. | 1976 | 1027440 | |
| studies on the bacteriophage ps8 of agrobacterium tumefaciens (smith and townsend) conn: physico-chemical properties of its dna. | dna from the bacteriophage ps8 was extracted and purified. the buoyant density was determined was 1.716 cm3/g. the guanine-cytosine content was calculated to be 57%. dna molecules which looked like circles were found among linear strands in an electron-microscopic study. with an endonuclease from streptomyces albus g the dna was digested to 19 fragments, with molecular weights ranging from 600 to 7,400 daltons. the molecular weight of the dna was determined to be 38.8 x 10(6) daltons +/- 8.7%. | 1976 | 1031838 |
| effect of some pesticides on the efficiency of the inoculated rhizobium associated with broad bean plants. | the effect of some pesticides on the efficiency of the inoculated rhizobium in broad bean plants (vicia faba) was studied in greenhouse experiments. different pesticides were used in this work, including insecticides, herbicides, nematocides, and a molecide in different concentrations. the study revealed the following conclusions. application of insecticides in different concentrations generally affected the formation of efficient nodules on the root system of broad bean plants. the inhibitory e ... | 1976 | 1037049 |
| effect of phosphate fertilization and seed inoculation with ""okadin'' at high rate on yield of broadbean and lentil. | the effect of phosphate fertilization and seed inoculation with ""okadin'' preperation of rhizobia strains on the yield of broadbean and lentil was tested in field experiments during the seasons 1969 to 1972 in the farm of faculty of agriculture, assiut university. superphosphate was added at the rate of 0, 50, 100, or 200 kg/fa., and ""okadin'' preparation, specific for the kind of legume planted, was applied to the seeds at 10 times the recommended rate. application of superphosphate at any of ... | 1976 | 1037057 |
| [factors of multiple resistance to antibiotics in nodule bacteria]. | multiple resistance to antibiotics (penicillin, levomycetin, neomycin, tetracycline) was found in 15% of collection strains of nodule bacteria and in strains isolated from natural environment. | 1976 | 1050635 |
| a search for a leghaemoglobin-like compound in root nodules of trema cannabina lour. | root nodules collected from the non-leguminous plant trema cannabina lour. grown under natural field conditions were examined for the presence of leghaemoglobin. no trace of any soluble haemoglobin-like compound could be detected by spectrophotometric analysis or molecular weight comparison studies, although a small amount of soluble haemoprotein with peroxidase activity was isolated. possible alternatives to the possession of a leghaemoglobin in non-legume/rhizobium n2-fixing associations are d ... | 1976 | 1247574 |
| [study of exopolysaccharides of the nitrogen-fixing nodular microorganism rhizobium lupini]. | 1976 | 1253695 | |
| on the isolation of ti-plasmid from agrobacterium tumefaciens. | an efficient lysis method for agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (ti)-plasmid. the lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,edta, followed by sarkosyl, after growth in the presence of carbenicillin. we also present a procedure for the isolation of the ti-plasmid on a large scale, that might be used for the mass isolation of other large plasmids which like the ti-plasmid, can ... | 1976 | 1257056 |
| glycerol metabolism in rhizobium. | four strains of rhizobium japonicum and one strain of r. trifolii were grown on glycerol and found to contain a soluble atp-glycerol kinase and a particulate glycerolphosphate dehydrogenase. both enzymes are induced by glycerol. the presence of nad+-or nadp+-glycerol dehydrogenase was not detected in any of the strains. no significant differences were found in the glycerol metabolic pathway between fast-and slow-growing rhizobia. | 1976 | 1260522 |
| morphology and host range of virulent phages of lotus rhizobia. | nineteen virulent bacteriophages for fast- and slow-growing rhizobia were isolated. most of the phage isolates were of two morphological types, and these showed specificity for either the fast- or the slow-growing rhizobia. the ecological distribution, morphology, and host range of the phages is presented. classification of lotus rhizobia is discussed on the basis of phage typing. | 1976 | 1260527 |
| evidence for diverse types of large plasmids in tumor-inducing strains of agrobacterium. | homology between the large plasmids of 15 pathogenic agrobacterium strains isolated from various parts of the world has been measured and was found to vary over a wide range, from 3 to 100%. two genetically distinct groups of plasmids can be identified: one closely related to the plasmid of a. tumefaciens a6, an octopine-utilizing strain, and the other closely related to the plasmid of a. tumefaciens c-58, a nopaline-utilizing strain. the plasmids of four agrobacterium strains do not belong to e ... | 1976 | 1262301 |
| genetic mapping of leucine and isoleucine-valine loci in rhizobium japonicum. | leucine and isoleucine-valine loci have been mapped in rhizobium japonicum. transformation analysis suggests a common pathway for isoleucine-valine biosynthesis. three-point reciprocal crosses indicated that all the leucine loci are not genetically linked. | 1976 | 1262320 |
| p-450 hemeproteins of rhizobium japonicum. purification by affinity chromatography and relationship to p-450cam and p-450lm-2. | 1976 | 1267795 | |
| octopine as a marker for the induction of tumorous growth by agrobacterium tumefaciens strain b6. | 1976 | 1267817 | |
| a new method for strain identification of rhizobium trifolii in nodules. | 1976 | 1270360 | |
| role of agrobacterium cell envelope lipopolysaccharide in infection site attachment. | lipopolysaccharide (lps) isolated from agrobacterium tumefaciens inhibited tumor induction by virulent bacteria. lps from site-binding strains was not effective if added to the plant wound shortly after the bacteria, and lps from avirulent, non-site-binding strains of agrobacterium was not inhibitory regardless of the order of addition. however, lps and whole cells of avirulent strains nt1 and iibnv6, which lack of agrobacterim virulence plasmid, were inhibitory. chromosomal deoxyribonucleic aci ... | 1976 | 1278998 |
| degradation of 4-aminopyridine in soil. | 1976 | 818143 | |
| ribonucleotide reductase in blue-green algae: dependence on adenosylcobalamin. | ten species of freshwater blue-green algae exhibit an adenosylcobalamin-dependent ribonucleotide reductase, thuse explaining the requirement for cobalt by these organisms. the evidence suggests a phylogenetic affinity between the cyanophytes and bacteria, such as clostridium and rhizobium, and the euglenoid flagellates, which also use the cofactor-dependent reductase. in contrast, the ribonucleotide reductase reaction in the few green algae surveyed shows no dependence on cobalamins. | 1976 | 818711 |
| discrimination of rhizobium japonicum, rhizobium lupini, rhizobium trifolii, rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate. | rhizobium strains (one each of rh. japonicum, rh. lupini, rh. leguminosarum) take up 2-ketoglutaric acid in general much faster and from lower concentrations in the medium than strains of escherichia coli, bacillus subtilis and chromobacterium violaceum. a strain of enterobacter aerogenes, however, is more similar to some rhizobium strains. the same strains of rhizobium take up also phosphate much faster and from lower concentrations than the other bacteria tested. 4 strains of rh. lupini proved ... | 1976 | 818969 |
| improved method for preparing anaerobic bacteroid suspensions of rhizobium leguminosarum for the acetylene reduction assay. | a method using ethylenediaminetetraacetate (edta) and toluene-treated pea bacteroid suspensions for the acetylene reduction assay is described. the high level of acetylene reduction by these bacteroids is comparable to that of intact plants. reproducibility of the edta-toluene treatment is, on the average, within 5%. preliminary experiments with soybeans indicate that the edta-toluene method might be applicable to other legumes as well. | 1976 | 820257 |
| [conditions for bacterial transfection]. | 1976 | 822657 | |
| [enzymatic reduction of legoglobin]. | during incubation in the buffer bacteroids rizobium lupini liberate an enzymic system (ferri-lb-reductase) capable of reducing ferri-lb and ferri-mb the hemoproteids reduction required the presence of cofactors (nadph or nadh). the ferri-lb-reductase activity was also found in the soluble fraction of nodules. it is assumed that bacteroids ferri-lb-reductase, maintains lb in a physiologically active reduced state into vegetable cells of the nodules "in vivo" as well. | 1976 | 826285 |
| chromosomal recombination and mapping in rhizobium leguminosarum. | 1976 | 826827 | |
| [competing ability of rhizobium trifolii strains and efficiency of clover nitraginization on spent peat mosses]. | the competing ability of active strains of clover nodule bacteria was assayed in vegetation and field experiments. the competing ability of the industrial strains 311a and 347a was found to be sufficiently high. these strains increased the yield of clover on exhausted turf peats of the kirovsk region by 26--29%. the stimulating effect was observed also with new strains 313b and 329b. the optimal dose for inoculation of clover seeds in these conditions is 50,000 nodule bacteria per one seed. inoc ... | 1976 | 135915 |
| [inhibitory analysis of the respiration of bacteroids from the nodules of yellow lupine]. | oxygen uptake and reduction of c2h2 by bacteroids was found to be inhibited by low concentrations of cyanide and azide. however, oxygen uptake was not completely suppressed even by 10(-3) m kcn. cyanide-resistant respiration was not inhibited by salicyl-hydroxamic acid, and seemed to be accomplished at the account of autoxidable flavo-proteins. a small light-reversible inhibition of respiration by carbon monoxide was found only in the bacteroids with a high rate of nitrogen fixation. rotenone, a ... | 1976 | 180385 |
| immunofluorescent polar tips of rhizobium japonicum: possible site of attachment or lectin binding. | rhizobium japonicum usda 31 demonstrated marked polarity by binding homologous fluorescent antibody (fa) heavily on one end of the cell. fa prepared against r. japonicum strains 110 and 138, and against r. trifolii ta1 cross-reacted with strain 31 only in the polar tip region. no polar immunofluorescing tips could be seen with fa against two other strains of r. japonicum or with those against several unrelated microorganisms. common antigens localized only in a polar region were seen in many rhi ... | 1976 | 56334 |
| [a rna extract from oncogenic and non oncogenic strains of agrobacterium tumefaciens is an indispensable element for the induction of tumors in datura stramomium]. | an rna bound to the reverse transcriptase of agrobacterium tumefaciens has been isolated and shown to be oncogenic for stem tissues of datura stramonium grown under axenic conditions. the tumorous nature of the cellular change induced by the infectious rna was demonstrated by serial grafts of tumors on datura stems and by cultivation of tumorous tissue in vitro on a medium without supplemental auxins and cytokinins. active cellular proliferation within tissues of datura stems was a prerequisite ... | 1976 | 58705 |
| a phenomenon of photoenhancement of acridine orange-induced fluorescence. | it is a common experience that, with exposure to exciting radiation of the fluorescence microscope, the acridine orange-induced red fluorescence of the nucleus, produced by feulgen hydrolysis, fades with a concomitant shift to green. the present investigation reports a phenomenon of photoenhancement observed in the hydrolyzed cytoplasm where pale green fluorescence increases in intensity with exposure to exciting radiation. the phenomenon has been noticed in rhizobium, oscillatoria, tomato root ... | 1976 | 58929 |
| the effect of rhizobiophages on the effectiveness of rhizobium meliloti in symbiosis with lucerne. i. effect of various strains of r. meliloti and rhizobiophages on the yield and nitrogen fixation in lucerne. | pot and test-tube experiments were carried out on the effect of phages on yield and nitrogen fixation in lucerne inoculated with effective and non-effective strains of rhizobium meliloti. it was found that some strains of r. meliloti became activated by the phages, which was reflected in an increase in yield and in crude protein content in the plants, while others lost their effectiveness. the results of pot experiments were in line with those of the test-tube experiments and have demonstrated t ... | 1976 | 59525 |
| the effect of rhizobiophages on the effectiveness of rhizobium meliloti in symbiosis with lucerne. ii. effects of phages on the r. meliloti strains during passing them through the plant. | studies were carried out of the effectiveness of rhizobium meliloti strains reisolated from the roots of lucerne inoculated with effective and non-effective strains in the presence or absence of rhizobiophages. estimates were made of the changes in phage-sensitivity and lysogeny found in these strains compared with the parent strains. it was found that the character of the reisolated strains depended on the changes brought about by passing through the plant and on the activity of the phages. | 1976 | 59526 |
| the occurrence of rhizobiophages in various lucerne plantations. | 1976 | 59531 | |
| proceedings: l-sorbose metabolism in agrobacterium: a biochemical explanation for an intrageneric phenotypic difference. | 1976 | 60964 |