Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [proceedings: plasmids in rhizobium japonicum]. | 1975 | 50812 | |
| loss of agglutinating specificity in stock cultures of rhizobium meliloti. | several strains of rhizobium meliloti which have been subcultured for 23-33 years have changed from being markedly specific in their somatic agglutination reactions to become widely cross-reactive. on the other hand a fresh collection of the same species obtained from naturally nodulated, field-grown plants revealed the high degree of agglutinating specificity which had previously characterised the old cultures. attempts to reselect a specific substrain from old cross-agglutinating cultures by s ... | 1975 | 50826 |
| proceedings: studies on large dna plasmids of agrobacterium tumefaciens. | 1975 | 54090 | |
| cross-reactive antigens and lectin as determinants of symbiotic specificity in the rhizobium-clover association. | cross-reactive antigens of clover roots and rhizobium trifolii were detected on their cell surfaces by tube agglutination, immunofluorescent, and radioimmunoassay techniques. anti-clover root antiserum had a higher agglutinating titer with infective strains of r. trifolii than with noninfective strains. the root antiserum previously adsorbed with noninfective r. trifolii cells remained reactive only with infective cells, including infective revertants. when adsorbed with infective cells, the roo ... | 1975 | 55100 |
| conversion of spheroplast symbiotes in a leafhopper, helochara communis fitch (cicadellidae: homoptera). | in mycetomes of leafhoppers, helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of dna--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. additional t-symbiotes were incorporated into the substance of these 'a-symbiotes'. | 1975 | 163130 |
| the nitrogen-fixing complex of bacteria. | 1975 | 164247 | |
| transfer of the tumor inducing factor in agrobacterium tumefaciens. | 1975 | 164858 | |
| hexopyranoside: cytochrome c oxidoreductase from agrobacterium. | 1975 | 165382 | |
| effect of adenine nucleotides on nad-dependent isocitrate dehydrogenases in rhizobia and bacteroids of legume root nodules. | atp, adp, amp and cyclic amp inhibit nad-dependent isocitrate dehydrogenase (l-s-isocitrate : nad-+ oxidoreductase, ec 1.1.1.41) from rhizobia but have no effect on the enzyme from corresponding bacteroids. this was observed using three rhizobial strains two of which are effective, and one ineffective, with lotus pedunculatus. using partially purified enzyme from each of the three rhizobial strains it was found that the adenine nucleotides inhibit the enzyme by competing with nad-+, not with iso ... | 1975 | 166683 |
| cyclic-amp phosphodiesterase activity in crown-gall tumor formation. | 1975 | 169845 | |
| purification and properties of cytochrome c-556 from agrobacterium tumefaciens b2a. | cytochrome c-556 from agrobacterium mefaciens b2a was isolated in a pure, homoneous state. the best purification procedure volved ammonium sulphate fractionation, delting on sephadex g-25, column chromatographic fractionation on deae- and cm-cellulose, and gel filtration on sephadex g-75 superfine. substitution of the cm-cellulose step by isoelectric focusing was successful. the purity of the final preparation is warranted by the purity index value, the electrophoretic patterns in the absence an ... | 1975 | 170180 |
| a comparison of dna from free living and endosymbiotic rhizobium leguminosarum (strain pre). | 1. bacteroids of rhizobium leguminosarum (strain pre) purified from root nodules of pisum sativum (var. 'rondo') by the standard procedure of differential centrifugation contained considerable contamination of mitochondrial material. this could be removed by incubation of the bacteroid preparation with 1 m kcl/1% deoxycholate. 2. the dna content of bacteroid cells of r. leguminosarum was found to have increased about three fold in comparison with the dna content of free living r. leguminosarum b ... | 1975 | 172157 |
| lipopolysaccharide as receptor for rhizobium phage 1p. | 1975 | 172600 | |
| a ferrodoxin from agrobacterium tumefaciens. | 1975 | 179857 | |
| [symbiotic efficiency in spontaneous mutants of rhizobium legumino-sarum resistant to streptomyocin, spectinomycin, or kanamycin]. | symbiotic effectiveness of 45 mutant strains selected from four wild effective strains of rhizobium leguminosarum for resistance to streptomycin, spectinomycin or kanamycin was determined on vicia faba. loss of effectiveness occurred in twenty of these mutants; distribution of ineffective mutants was uniform among the three types of antibiotic resistant mutants but varied with the parent strain from which mutants have been derived. | 1975 | 125155 |
| carbohydrate catabolism of selected strains in the genus agrobacterium. | radiorespirometric and enzyme analyses were used to reveal the glucose-catabolizing mechanisms functioning in single strains of seven presumed agrobacterium species. the entner-doudoroff and pentose cycle pathways functioned in a. radiobacter, a. tumefaciens, a. rubi, and a. rhizogenes. whereas both catabolic pathways were utilized to an almost equal degree in the a. radiobacter and a. tumefaciens strains, use of the entner-doudoroff pathway predominated in the a. rubi and a. rhizogenes strains. ... | 1975 | 128316 |
| nitrogen fixing activity in rhizobium japonicum separated from plant cell cultures. | induced by soy bean tissue cultures in socalled "tissue chambers", rhizobium japonicum str. 61-a-96 developed nitrogenase activity separated from the plant cells. the activity proceded for 48 h with a rate of 1 x 10(-8) nmol c2h4h-1 cell-1, which is about 6% of the activity measured for bacteroids from rhizobium japonicum in nodules of glycine max. | 1975 | 130001 |
| [nucleic acids during tumorous transformation in plants]. | crown-gall, one of the "plant cancer" is induced in the presence of a soil bacterium agrobacterium tumefaciens which elaborates a tumor inducing principle (t.i.p.), the nature of which is unknown. several informations suggest that some dna sequences of bacterial origin are included in tumorous cells of tissue cultures. a ribonuclease inhibits induction. an rna extracted from agrobacterium induces some hyperplasia transplantable by graft. | 1975 | 130189 |
| [tumorous nature of hyperplasia obtained experimentally]. | the determination of the tumorous nature of an overgrowth provides one of the most essential information for all research concerning animal and plant cancerisation. the experimental procedure which will be described here answers to this imperative: it allows to prove the tumorous characteristics of proliferations obtained by inoculation of agrobacterium tumefaciens rna fractions to datura stems. | 1975 | 130191 |
| ammonia assimilation by rhizobium cultures and bacteroids. | the enzymes involved in the assimilation of ammonia by free-living cultures of rhizobium spp. are glutamine synthetase (ec. 6.o.i.2), glutamate synthase (l-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ed i.4.i.4). under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of r. leguminosarum, r. trifolii and r. japonicum proceeded via glutamine synthetase and glutamate synthase. under glucose limitation and with an excess ... | 1975 | 234505 |
| [beta-hydroxybutyrate dehydrogenase of the bacteroid rhizobium lipid]. | 1975 | 235408 | |
| induction of glutamate synthase during nodule development in lupin. | 1975 | 237796 | |
| enzymes of ammonia assimilation in rhizobium leguminosarum bacteroids. | the activities of the following enzymes were studied in connection with dinitrogen fixation in pea bacteroids: glutamine synthetase(l-glutamate: ammonia ligase (adp-forming)(ec 6.3.1.2)(gs); glutamate dehydrogenase (nadp+)(l-glutamate: nadp+ oxidoreductase (deaminating)(ec 1.4.1.4)(gdh); glutamate synthase (l-glutamine: 2-exeglutarate aminotransferase (nadph-oxidizing))(ec 2.6.1.53)(gogat). gs activity was high throughout the growth of the plant and gogat activity was always low. it is unlikely ... | 1975 | 238731 |
| adsorption and selection of rhizobia with ion-exchange papers. | ion exchange papers were used to study the adsorption of 32p-labelled rhizobia on defined surfaces. two strains of rhizobium japonicum and one each of r. leguminosarum and r. lupini were compared with escherichia coli and bacillus subtilis. the ratio of adsorption to strong and to weak acid papers/strong and weak basic papers was consistantly higher for all rhizobial strains compared to the other bacteria. the process of desorption by increasing the ion-concentration causes about 35% desorption ... | 1975 | 242293 |
| immunological evidence for the capability of free-living rhizobium japonicum to synthesize a portion of a nitrogenase component. | immunodiffusion tests conducted under aerobic conditions demonstrated that cross-reactive material to antiserum prepared against the mo-fe protein component of nitrogenase from soybean nodule bacteroids was detectable in extracts of free-living rhizobium japonicum cells cultured in a standard medium under: aerobic conditions; aerobic conditions with nitrate; aerobic conditions with ammonia; anaerobic conditions with nitrate; and anaerobic conditions with nitrate and ammonia. the most intense pre ... | 1975 | 803382 |
| [regulation of nitrogenase biosynthesis in microorganisms]. | 1975 | 803994 | |
| protozoa and the decline of rhizobium populations added to soil. | a fall in rhizobium abundance occurred in nonsterile soil inoculated with large numbers of the root-nodule bacteria, but many of the rhizobia still survived. no such decline was evident in sterile soil. protozoa feeding on these bacteria were isolated from soil and other environments. as the abundance of rhizobium meliloti and a cowpea rhizobium strain in soil decreased, the protozoan density increased. the inability of the predators to eliminate their prey from soil was not the result of the pr ... | 1975 | 807307 |
| [cytokinins in microorganisms]. | 1975 | 808806 | |
| antigenic differences between infective and noninfective strains of rhizobium trifolii. | immunodiffusion and immunoelectrophoresis techniques have revealed the presence of soluble antigens in sonicated preparations of four infective strains of rhizobium trifolii which were absent in similar preparations of related noninfective mutants derived from the infective strains. the soluble antigens unique to the infective strains were cross-reactive with one another. | 1975 | 809004 |
| on the question of the integration of exogenous bacterial dna into plant dna. | extensive studies with pea, tomato, and barley failed to confirm the evidence presented by previous investigators for integration or replication of exogenously applied bacterial dna in these plants. labeled dna of buoyant density in cscl intermediate between that of high density donor bacterial dna and of plant dna was never observed with axenic plants. intermediate peaks, similar to those used as evidence for recombination by earlier investigators, were observed only when the plants were contam ... | 1975 | 809769 |
| [an current question in the molecular genetics of bacteria]. | the author considers the possibilities and limits of extrapolation of the data by the genetics of one species of bacteria to the other. it is emphasized that even in the related bacterial species a similar localization on chromosomes was inherent only to some of the unitypical genes, by in this case as well not all the genes were grouped in the same way, and differed by their delicate structure. an idea on the significant role of genetic metabolism in the microbial evolution is being developed; ... | 1975 | 813460 |
| studies on phage 1p receptors in rhizobium trifolii and rhizobium leguminosarum. | the rate of phage 1p attachment to rhizobium cell walls was increased in the presence of ethylenediaminetetraacetic acid (edta). on the other hand the rate of adsorption of phage 1p to the triton -- insoluble cell walls was diminished. the subsequent treatment of cell walls with 2% triton x-100 and 5mm edta caused a more substantial decline of the phage inactivating capacity. lipopolysaccharides (lps) isolated from the sensitive strains, contrary to those from phage-resistant mutants, inactivate ... | 1975 | 813494 |
| [immobilization of rhizobium lupini bacteroids in polyacrylamide gel]. | 1975 | 1093828 | |
| production of cellulose microfibrils by rhizobium. | electron microscope examination of rhizobium spp. revealed microfibrils produced by flocculating strains but not by nonflocculating strains. the microfibrils from r. trifolii (na30) were isolated and identified as cellulose by enzymatic, x-ray diffraction, and infrared spectral analyses. both infective and noninfective strains of r. trifolii flocculated and produced microfibrils. more infection threads were observed in clover root hairs growing in the presence of flocs in comparison with root ha ... | 1975 | 1096821 |
| crown gall tumors: are bacterial nucleic acids involved? | 1975 | 1100042 | |
| regulation and genetics of bacterial nitrogen fixation. | 1975 | 1101806 | |
| methods for growing nitrogen-fixing bacteria separated from plant cells. | 1975 | 1104122 | |
| an enrichment technique for auxotrophs of agrobacterium tumefaciens using a combination of carbenicillin and lysozyme. | a procedure to enrich for auxotrophic and fermentation mutants of agrobacterium tumefaciens is described. the method is based on the amplification of the killing power of carbenicillin by the addition of lysozyme. isolation frequencies of some types of mutants are presented, with and without the application of the proposed procedure. the yield of mutants is usually enhanced a hundredfold per enrichment treatment. | 1975 | 1104767 |
| [rapid method of determining the capacity for symbiotic nitrogen fixation in growing plants under sterile and under natural conditions]. | 1975 | 1107764 | |
| attempts to induce tumours with nucleic acid preparations from agrobacterium tumefaciens. | nucleic acid preparations from agrobacterium tumefaciens (smith & townsend) conn. have been tested for tumorigenic activity on a number of bioassay systems including carrot root explants, sunflower and tobacco stem segments, callus cultures of sunflower, tobacco and carrot, and sunflower stems. the methods used to isolate and test the dna included those which have been reported to be successful for the induction of tumours. strict precautions were taken to ensure that the dna samples used in the ... | 1975 | 1113080 |
| host-phage interaction on agrobacterium tumefaciens. iv. phage-directed protein synthesis. | gel electrophoretic and autoradiographic techniques were used to detect the temporal sequence of protein synthesis after infection of the sensitive strain agrobacterium tumefaciens with phage lv-1. three classes of protein were detected: early proteins, class i, which include a protein capable of shutting off host protein synthesis; class ii, proteins which are detected after 30 min; and late proteins, class iii, which include the phage-directed endolysin and five additional proteins that appear ... | 1975 | 1113375 |
| variation in colony characteristics and symbiotic effectiveness of rhizobium. | 1975 | 1116979 | |
| influence of combined nitrogen on the symbiosis between single colony isolates of rhizobium cb756 and macrotyloma axillare. | 1975 | 1116982 | |
| [microbiologic dehydrogenation of microcrystalline delta4-3-ketosteroids]. | 1975 | 1117085 | |
| characterization of different plaque-forming and defective temperate phages in agrobacterium. | four agrobacterium tumefaciens temperate phages (pb2a, pb6(omega), pv-1(lv-1) and ps8), were shown to have the same genome size. moreover hybridization experiments by the heteroduplex method and electron microscopy showed a 100% homology between these four phage genomes. indications for lysogeny were found by direct means for the agrobacterum timefaciens strain 396, agrobacterium radiobacter strain 8149 and agrobacterium species 0362 and by the electron microscope negative staining technique fo ... | 1975 | 1123610 |
| attempts to detect agrobacterium tumefaciens and bacteriophage ps8 dna in crown gall tumors by dna-dna-filter hybridization. | a systematic study of the dna-dna-filter reaction is presented which measures its ability to detect small amounts of simple dna (bacterial or bacteriophage) in model mixtures of dna immobilized on filters. saturation curves show qualitatively that significant binding occurs when there is 10% agrobacterium tumefaciens dna on the filter but not 1%. ps8 bacteriophage dna is detectable at a level of 0.1%. true saturation is not attained in the bacterial dna reaction : radioactivity bound represents ... | 1975 | 1125316 |
| some unusual fatty acids of rhizobium. | a number of unusual fatty acids were identified after isolation from rhizobium. they include 11-methyl-octadec-11-enoic, 12-methoxy-11-methyl- and 11-methoxy-12-methyloctadecanoic, and 11-methoxy- and 13-methoxynonadecanoic acids. | 1975 | 1128167 |
| polarographic determination of 3-keto sugars-1. | 1975 | 1128262 | |
| protein turnover measured by 18-o exchange with h2-18o in non-growing cells of agrobacterium tumefaciens. | 1975 | 1133576 | |
| acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. | 1975 | 1134573 | |
| physical evidence of a plasmid in rhizobium japonicum. | 1975 | 1140246 | |
| plasmid required for virulence of agrobacterium tumefaciens. | the irreversible loss of crown gall-inducing ability of agrobacterium tumefaciens strain c-58 during growth at 37 c is shown to be due to loss of a large plasmid (1.2 x 10-8 daltons). the gene responsible for this high rate of plasmid loss at elevated temperatures seems to be located on the plasmid. in addition, another spontaneous avirulent variant, a. tumefaciens strain iibnv6 is shown to lack the virulence plasmid which its virulent sibling strain, iibv7, possesses. deoxyribonucleic acid reas ... | 1975 | 1141196 |
| identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. | improved methods for the identification and grouping of bacteria by polyacrylamide gel electrophoresis of soluble proteins are described. electrophoretic protein patterns were obtained in rigorously standardized comditions. the results were much more reproducible than any described previously. some of the factors affecting reproducibility were; growth conditions, time and speed of centrifugation of extracts, and conditions of gel electrophoresis. protein patterns were compared by computing corre ... | 1975 | 1141858 |
| identification of the rhizobium strains in pea root nodules using genetic markers. | pea plants were inoculated jointly with pairs of genetically marked strains of rhizobium leguminosarum. out of 297 modules examined 56 contained both inoculant strains. the ratios of the strains in the inoculum did not affect the frequencies of mixed nodules. generally one of the strains consistently occupied the majority of the nodules and ithe mixed nodules comprised the majority of bacteria. transfer of the p-group r factor, rp4, between certain strains of rhizobium within mixed nodules was d ... | 1975 | 1141859 |
| isolation and characterization of a rod-shaped bacteriocin from a strain of rhizobium. | a bactericidal agent ('bacteriocin 16-2') produced by rhizobial strain 16-2 had been characterized as a sheathless rod-shaped particle with a length of 200 nm and a diam. of 8 nm. one end of the rod is pointed and carries short fibre-like appendages, while the other end appears square. the particles specifically adsorb with their pointed end to bacteriocin-sensitive, but not to bacteriocin-resistant, cells. the possible mode of action of this bacteriocin in discussed. | 1975 | 1141864 |
| a genetic model for pathogenicity in agrobacterium and for tumour induction in plants. | 1975 | 1142794 | |
| plant genotype times rhizobium strain interactions in white clover. | 1975 | 1147320 | |
| studies on agrobacterium tumefaciens. iv. nonreplication of the bacterial dna in mung beam (phaseolus aureus). | 1975 | 1147920 | |
| [regulation of nitrogenase from lupin bacteroids]. | 1975 | 1149612 | |
| agrocin 84 sensitivity: a plasmid determined property in agrobacterium tumefaciens. | it was shown for some oncogenic agrobacterium tumefaciens strains that agrocin 84 sensitivity is determined by the presence of a large closed circular dna plasmid, called the ti-plasmid. whereas wild-type strain c58 is agrocin 84 sensitive, all ti-plasmid cured derivatives were found to be fully resistant. moreover all independently isolated agrocin 84 resistant colonies were stably non-oncogenic and plasmid negative. in a growth experiment carried out at 37 degrees c it was shown that the ki ... | 1975 | 1152843 |
| the occurrence of twitching motility among gram-negative bacteria. | almost 1000 strains representing well above 50 different species or groups of gram-negative bacteria were examined for twitching motility. this kind of motility was mainly found in strictly aerobic cocci and rods (viz. acinetobacter calcoaceticus, moraxella spp., neisseria gonorrhoeae, n. meningitidis, pseudomonas spp., phenon 3 of thornley that is closely related to acinetobacter, and marine, yellow-pigmented rods), but also in the facultative eikenella corrodens and in anaerobic strains presum ... | 1975 | 1155115 |
| electron microscopic characterization of rhizobium bacteriophage 16-6-12 and its isolated deoxyribonucleic acid. | bacteriophage 16-6-12 of rhizobium lupini has a long, non-contractile tail and a head which is hexagonal in outline. the tail is 140 nm in length, 11 nm in diameter, and carries a short term fiber. analysis of the tail structure by optical diffraction indicates that it is of the helical "stacked disc" type. after phenol-extraction from purified particles, the dna of phage 16-6-12 can circularize in vitro. no significant difference in contour length was observed between the linear (14.34 plus or ... | 1975 | 1156101 |
| growth dynamics of agrobacterium tumefaciens in chemostat cultures limited by carbon source and mineral nutrients. | agrobacterium tumefaciens was grown in a chemostat in a chemically-defined medium which hs alpha-methyl d-glucoside, magnesium, manganese, phosphate or urea as the growth-limiting nutrient. steady-state biomass concentrations were dependent on the specific growth rate of the organism when alpha-methyl d-glucoside, manganese or phosphate were growth-limiting nutrients. during magnesium-limited growth, large undamped oscillations in biomass concentration occurred. in all chemostat cultures a varia ... | 1975 | 1156102 |
| acetylene reduction by pure cultures of rhizobia. | acetylene reduction has been demonstrated in pure cultures of rhizobia. the requirements and conditions necessary for the activity in rhizobium sp. 32h1 are described. the most important factors are a low cell density and a very low oxygen concentration. | 1975 | 1158849 |
| interactions between azotobacter and "phosphobacteria" and their establishment in the rhizosphere as affected by soil fertility. | the effects on plant growth of "bacterial fertilizers" prepared from azotobacter spp. and phosphate-solubilizing bacteria ("phosphobacteria") have been the subject of much controversy. cases where no plant-growth stimulation occurred may often be accounted for by the failure to establish the bacterial inocula in the rhizosphere. three factors that may influence inocula establishment, i.e. soil fertility, manuring, and interactions between azotobacter and "phosphobacteria," were examined in pot e ... | 1975 | 1164695 |
| action of water in depressing acetylene reduction by detached nodules. | adverse effects of water on acetylene reduction by detached soybean root nodules could not be attributed to ethanol formation, but apparently were associated with nodule tissue damage caused by detachment. intact nodules were not affected by moisture. | 1975 | 1167777 |
| regulation of predation by prey density: the protozoan-rhizobium relationship. | tetramitus rostratus and strains of hartmanella, naegleria, and vahlkampfia consumed large numbers of rhizobium meliloti cells in a salt solution, but protozoan multiplication and the bacterial decline stopped when the prey density fell to about 10-6 to 10-7 cells/ml. at higher prey densities, the maximum numbers of hartmanella sp. and naegleria sp. were proportional to the quantity of r. meliloti initially provided to the amoebas. when supplemental rhizobia were supplied to hartmanella sp. or n ... | 1975 | 1168441 |
| structural similarity of the membrane envelopes of rhizobial bacteroids and the host plasma membrane as revealed by freeze-fracturing. | the freeze-fracture technique was used to study the host plasma membrane and the membrane envelope of bacteroids in rhizobial root nodules of three host-rhizobium combinations. in all three combinations studied, the membrane envelopes of bacteroids are structurally similar to their host plasma membrane. however, the membrane appears to be reversed, because the number and arrangement of particles in the outer fractured face (face a, concave) and in the inner fractured face (face b, convex) of the ... | 1975 | 1168633 |
| acetylene reduction by transfilter suspension cultures of rhizobium japonicum. | 1975 | 1169944 | |
| involvement of oxyleghaemoglobin and cytochrome p-450 in an efficient oxidative phosphorylation pathway which supports nitrogen fixation in rhizobium. | cellular atp level, atp/adp ratio and nitrogenase activity rise when oxyleghaemoglobin is added to respiring suspensions of rhizobium japonicum bacteroids from soybean root nodules. increased gaseous o2 tension is much less efficient than oxyleghaemoglobin in stimulation of bacteroid atp production. studies with the inhibitor carbonyl cyanide m-chlorophenylhydrazone show this atp to be generated as a consequence of oxidative phosphorylation. n-phenylimidazole, a specific cytochrome p-450 inhibit ... | 1975 | 1169973 |
| nitrogen fixation by free-living rhizobium in a defined liquid medium. | 1975 | 1170865 | |
| nitrate reductase from bacteroides of rhizobium japonicum: enzyme characteristics and possible interaction with nitrogen fixation. | the soluble nitrate reductase of rhizobium japonicum bacteroids has been purified and its properties compared to those of aerobically grown cells. the enzymes from both sources are similar with molecular weights of about 70 000 suggesting no close relationship with the molybdo-protein component of nitrogenase. nitrite, the product of nitrate reductase, strongly inhibited the nitrogenase activity from bacteroids, at concentrations less than 100 mum. thus, an interference in the rate of nitrogen f ... | 1975 | 1170894 |
| role of pectic and cellulolytic enzymes in the invasion of the soybean by rhizobium japonicum. | past workers have suggested pectic enzyme involvement in the invasion of legumes by rhizobium. however, no role for pectic acid, pectin, or methyl cellulose depolymerase enzymes in the invasion of r. japonicum was suggested by the current study. seedling inoculation with infective bacteria did not result in increased enzyme activity. rhizobium japonicum cell-free culture extracts and 3-indoleacetic acid did not affect the activation, induction, or binding of these enzymes. | 1975 | 1172457 |
| inhibition of protein synthesis by d-threo-chloramphenicol in the laboratory and nodule forms of rhizobium lupini. | protein synthesis by both laboratory-grown bacteria and isolated nodule bacteroids of rhizobium lupini (strain wu8) is inhibited by d-threo-chloramphenicol, the bacteroid form being the more sensitive to the antibiotic. a comparison between the two forms of the uptake of [14c]chloramphenicol showed that the bacteria always attained a lower intracellular chloramphenicol concentration. it is proposed that the sensitivity difference is due to a difference in membrane permeability between the two fo ... | 1975 | 1176963 |
| the genus agrobacterium and plant tumorigenesis. | 1975 | 1180518 | |
| fragmentation analysis of extracellular acid polysaccharides from seven rhizobium strains. part i. d-glucuronic acid-containing oligosaccharides. | the extracellular, bacterial polysaccharides from seven rhizobium strains have been submitted to partial hydrolysis with acid. several neutral oligosaccharides, some containing pyruvic acid, were isolated together with d-glucuronic acid-containing oligosaccharides. the polysaccharide from rh. meliloti did not contain glucuronic acid. for the other six strains, the following components were characterized: 4-o-(beta-d-glucopyranosyluronic acid)-d-glucuronic acid, 4-o-(beta-d-glucopyranosyluronic a ... | 1975 | 1182711 |
| frequency of infection and efficiency of transfection of rhizobium meliloti cells and spheroplasts. | competent cultures of rhizobium meliloti cells and spheroplasts obtained by various methods were infected with dna of phage 1a. the frequency of infection among the cells and spheroplasts was 2 x 10(-8)-5 x 10(-10). the efficiency of transfection calculated from the ratio of plaque forming units to infective dna molecule of phage 1a was 5 x 10(-8) to 10(-10). frequency of infection and efficiency of transfection among the competent cells were by one order of magnitude higher in the case of the s ... | 1975 | 1189991 |
| the ribosomal ribonucleic acid of agrobacterium tumefaciens. | the 23s rrna of agrobacterium tumefaciens contains at least two nicks which result in the formation of rna components with mol.wts. of 0.52 x 10(6) and 0.48 x 10(6). thus under the usual conditions of extraction and analysis, no 23s rrna was recovered from the bacterium. the experiments show that 23s rrna is synthesized as a continuous chain, in which one or two nicks are formed almost immediately near the ends of the molecule and an additional nick in the middle at a later time. | 1975 | 1191256 |
| structure and biosynthesis of the ribosomal ribonucleic acids from the oncogenic bacterium agrobacterium tumefaciens. | the rrna of the oncogenic bacterium agrobacterium tumefaciens was extracted by several methods and analysed by polyacrylamide-gel electrophoresis. the large rrna of this bacterium is degraded in vivo during the maturation of the ribosome. the influence of mg2+ and denaturation on degradation of 23s rna was studied. in pulse and chase experiments, we identified two precursors of the rrna with mol.wts. of 1.04 x 10(6) and 0.70 x 10(6). from studies of the structure of the large rrna, we propose th ... | 1975 | 1191257 |
| toxicity of sodium and chloride ions to rhizobium spp. in broth and peat culture. | 1975 | 1194132 | |
| loss of symbiotic capacity in commercially useful strains of rhizobium trifolii. | 1975 | 1194136 | |
| the effect of the ratio of magnesium: calcium on numbers of rhizobium in liquid medium. | 1975 | 1194137 | |
| on the question of integration of agrobacterium tumefaciens deoxyribonucleic acid by tomato plants. | treatment of tomato plants with agrobacterium tumefaciens causes subsequently administered [3h]thymidine to be preferentially incorporated into a satellite deoxyribonucleic acid (dna) whose buoyant density is between that of bacterial dna (rho = 1.718 g/cm3) and plant main band dna (rho = 1.692 g/cm3). satellite dna upon shearing or sonic treatment releases fragments of higher and lower buoyant density, as reported by earlier investigators. the satellite has no significant base sequence homology ... | 1975 | 1194235 |
| factors influencing the formation and stability of d-glucoside 3-dehydrogenase activity in cultures of agrobacterium tumefaciens. | d-glucoside 3-dehydrogenase specific activity in agrobacterium tumefaciens was maximal towards the end of the exponential growth phase of batch cultures; over 90% of the activity disappeared within the next 15 h. manganese ions, although essential for growth of the organism, strongly repressed d-glucoside 3-dehydrogenase synthesis in sucrose medium but had little effect when the carbon source was methyl alpha-d-glucoside. d-glucoside 3-dehydrogenase activity increased linearly with increasing sp ... | 1975 | 1194891 |
| effect on microorganisms of volatile compounds released from germinating seeds. | volatile compounds evolved from germinating seeds of slash pine, bean, cabbage, corn, cucumber, and pea were evaluated for their ability to support growth of microorganisms in liquid mineral salts media lacking a carbon source. growth of eight bacteria was measured turbidimetrically and of six fungi as dry weight of mycelium. volatiles caused increased growth of pseudomonas fluorescens, bacillus cereus, erwinia carotovora, agrobacterium tumefaciens, a. radiobacter, rhizobium japonicum, mucor muc ... | 1975 | 1201509 |
| exopolysaccharide depolymerases induced by rhizobium bacteriophages. | enzymes induced by two rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most r. trifolii and r. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of r. meliloti, the slow-growing rhizobia, or agrobacterium. ca2+ and (or) mg2+ were required for enzyme activity. in all strains tested, depolymerization of exopolysaccharide occurred when there was successful phage infection, but depolymerization also occurred with exopolysacchari ... | 1975 | 1201510 |
| the orientation of certain root-nodule bacteria at interfaces, including legume root-hair surfaces. | 1975 | 1202159 | |
| plasmid deoxyribonucleic acid in rhizobium vigna and rhizobium trifolii. | 1975 | 1203260 | |
| [synthesis and breakdown of poly-beta-hydroxybutyric acid in rhizobium lupini]. | the effect of various substrates on synthesis and decomposition of poly-beta-hydroxybutyric acid (phba) was studied in cell suspensions of the effective strain of rhizobium lupini and in suspensions of the bacteroids of this strain which were isolated from lupine nodules at different growth stages of the plants. glucose and beta-hydroxybutyrate were found to be the best substrates for synthesis of phba in all variants. the content of phba in the presence of these substrates increased in suspensi ... | 1975 | 1207500 |
| [effect of ultraviolet rays on the activity of clover nodule bacteria]. | the effect of uv on the survival, morphological variability, and effectiveness was studied with nodule bacteria of clover, strain 374a. the survival of the strain directly depended on the dose of uv rays, being 43.3% at 130 erg/mm2 and 0.000022% at 22,900 erg/mm2. uv irradiation yielded two morphological types of colonies; they differed by the production of slime which was less at higher doses of uv (15,970 to 22,900 erg/mm2). variants with either positive or negative effectiveness were obtained ... | 1975 | 1207509 |
| [possibility of the effect of symbiosis on the dna of nodule bacteria]. | 1975 | 1207523 | |
| [effect of cultivation conditions on the accumulation of poly-beta-hydroxy-butyric acid in rhizobium lupini]. | the influence of the age of the culture and nitrogen source on the accumulation of poly-beta-hydroxybutyric acid by different strains of rhizobium lupini was studied. the accumulation depended on the age of the culture and reached maximum at the end of the logarithmic and at the beginning of the stationary phase of the bacterial growth (about 50-60% dry weight). the accumulation varied in relation to the nitrogen source used: it was the highest in the glutamate medium and the lowest on nitrate n ... | 1975 | 1208373 |
| [a study of polysaccharides in fast growing "rhizobium" (author's transl)]. | this work is an attempt to use the composition of exopolysaccharides in the rhizobium genus as a criterium of taxonomic importance. the organisms were first cultured in media and under conditions which led to the production of as many polysaccharides as possible. the medium chosen for proliferation conditions was wright's medium; for conditions of non-proliferation conditions was wright's medium; for conditions of non-proliferation, a minimal medium without nitrogen was used. the polysaccharides ... | 1975 | 1211720 |
| ultrastructure of rhizobium-induced infection threads in clover root hairs. | ultrastructural studies of rhizobium-induced infection threads in clover root hairs show that the infection thread is initiated by an invagination process. root hair wall growth is redirected at a localized point, resulting in the formation of an open pore. there is no direct penetration through the wall, and the bacteria remain extracellular within the root hair. | 1975 | 1211931 |
| [effect of inoculation on nitrogen fixation by chick pea, on its crop and protein content]. | active strains of rh. cicer were found to increase the crop of chick pea by 25--36%, the content of protein by 2--6%, and the content of total nitrogen in the roots by 1.7--2%. symbiotic fixation of nitrogen determined by a technique of "inoculation" varies within 40--60% depending on the activity of the strain. no correlation has been established between the effectiveness of the strains of nodule bacteria and the activity of their enzymes--dehydrogenases. | 1975 | 1214612 |
| [synthesis of thiamine and riboflavin by nodule bacteria of clover and lupine]. | 1975 | 1214651 | |
| [activity of dehydrogenases in lupine nodule bacteria]. | 1975 | 1214668 | |
| [synthesis of pyridoxine and cobalamin by nodule bacteria clover and lupine]. | 1975 | 1214669 | |
| [group b vitamins in nodules of lupine inoculated with active and less active strains of nodule bacteria]. | 1975 | 1219321 | |
| insecticides and soil microorganisms. iii. fate of 14c-labelled dipterex as affected by two nodule-forming rhizobium spp. and roots of their respective leguminous host plants. | chromatographic analysis led to the identification of monomethyl- and dimethyl-phosphates as metabolites resulting from the enzymatic degradation of 14c-labelled dipterex in the buffer solutions and root tissues of broad bean and clover plants, as well as in the culture media of rhizobium leguminosarum and rhizobium trifolii. the formation of 14co2 from rhizobial cultures containing radioactive dipterex suggests that some of the liberated methanol groups (during breakdown of dipterex) are oxidat ... | 1975 | 1220485 |