Publications

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effect of additional shots on growth of rhizobium trifolii in relation to mass cultivation of rhizobia. 19734613718
factors affecting the reduction of acetylene by rhizobium-soybean cell associations in vitro.the acetylene reduction assay was used to measure presumed n(2)-reducing activity in rhizobium-soybean cell associations in vitro. no acetylene reduction was observed in liquid suspensions of these organisms, but cells plated onto an agar medium from a liquid suspension of rhizobium and soybean cells exhibited acetylene-dependent production of ethylene after 7 to 14 days. aggregates of soybean cells 0.5 to 2.0 mm in diameter were required for this activity. decreasing oxygen from 0.20 atm to 0.1 ...197416658654
a simple technique for the establishment of nitrogenase in soybean callus culture.a nitrogen-fixing association can be readily established in vitro between rhizobium and cultured soybean root cells. plant cells are grown as a thin callus on the surface of solid defined medium containing low levels of inorganic nitrogen and inoculated with bacteria during the active phase of growth. acetylene reduction activities up to 275 nmoles ethylene/hr.g dry weight of cells have been recorded.the system is compact, uses inexpensive glassware, and facilitates multiple replication of sampl ...197416658659
the flavin content of clovers relative to symbiosis with a riboflavin-requiring mutant of rhizobium trifoli.a riboflavin-requiring auxotroph of rhizobium trifolii (t1/d-his(r)-15) formed ineffective root nodules on red clover and on two cultivars of subterranean clover, but produced almost fully effective nodules on several other cultivars of subterranean clover. fluorescence and bioassay measurements of the flavin content of the roots and shoots of these cultivars revealed no differences between cultivars which could be correlated with the differences in symbiotic response. the concentration of flavi ...197416658676
promotion of acetylene reduction by rhizobium-soybean cell associations in vitro.nitrogenase activity, determined by the acetylene reduction assay, in soybean cell suspensions infected with rhizobium, was stimulated significantly by the addition of succinic acid or glutamine. acetylene-dependent ethylene values as high as 4.6 mumoles of ethylene/gram dry weight.hour were observed.197416658946
lectins: a possible basis for specificity in the rhizobium--legume root nodule symbiosis.soybean lectin labeled with fluorescein isothiocyanate combined specifically with all but 3 of 25 strains of the soybean-nodulating bacterium rhizobium japonicum. the lectin did not bind to any of 23 other strains representative of rhizobia that do not nodulate soybeans. the evidence suggests that an interaction between legume lectins and rhizobium cells may account for the specificity expressed between rhizobia and host plant in the initiation of the nitrogen-fixing symbiosis.197417812054
pristionchus lheritieri as a carrier of rhizobium japonicum. 197419308114
[symbiotic fixation of molecular nitrogen]. 19744618273
[natural electron donors in the process of nitrogen fixation]. 19744619170
the carriage of rhizobium by leguminous seeds after floral spray. 19744619550
[change in specificity of pea nodule bacteria mutants]. 19744495004
[makeup of the cytochromes of the bacteroid rhizobium leguminosarum (vicia faba)]. 19744374257
microbial metabolism of the pyridine ring. the metabolism of pyridine-3,4-diol (3,4-dihydroxypyridine) by agrobacterium sp.1. pyridine-3,4-diol (3,4-dihydroxypyridine, 3-hydroxypyrid-4-one), an intermediate in 4-hydroxypyridine metabolism by an agrobacterium sp (n.c.i.b. 10413), was converted by extracts into 1mol of pyruvate, 2mol of formate and 1mol of nh(3) at ph7.0. 2. formate, but not the alternative likely product formamide, was further oxidized fivefold faster by 4-hydroxypyridine-grown washed cells than by similar organisms grown on succinate. 3. the oxidation of pyridine-3,4-diol by crude extracts at ph8.5 ...19744375963
microbial metabolism of the pyridine ring. the hydroxylation of 4-hydroxypyridine to pyridine-3,4-diol (3,4-dihydroxypyridine) by 4-hydroxypyridine-3-hydroxylase.1. the first metabolic step in the biodegradation of 4-hydroxypyridine by an agrobacterium sp. was hydroxylation to form pyridine-3,4-diol. 2. extracts required 1mol of o(2) and 1mol of nadh or nadph for the conversion of 4-hydroxypyridine into pyridine-3,4-diol, suggesting that the enzyme responsible, 4-hydroxypyridine-3-hydroxylase, was a mixed function mono-oxygenase. 3. after treatment with acidic (nh(4))(2)so(4) the enzyme required fad for activity; fmn and riboflavin would not substitute f ...19744156169
on a eukaryotic cell-transformation by a bacterium. 19744597805
[a method of separating slowly growing strains of nodular bacteria from slime]. 19744598406
r factor transfer in rhizobium leguminosarum. 19744612098
chemical analysis of exocellular, acid polysaccharides from seven rhizobium strains. 19744363665
isolation of the tumor-inducing rna from oncogenic and nononcogenic agrobacterium tumefaciens.two rna fractions have been isolated and purified from both oncogenic and nononcogenic strains of agrobacterium tumefaciens. both rnas are capable of inducing the formation of transplantable tumors when introduced at wound sites in stems of datura stramonium plants. one of these rna fractions was found to be bound to an rna-directed dna polymerase, while the other was associated with the bacterial dna. physical evidence suggests that both are single stranded and small in size; linear sucrose gra ...19744525450
agrobacterium tumefaciens dna and ps8 bacteriophage dna not detected in crown gall tumors.renaturation kinetics of labeled agrobacterium tumefaciens dna are not influenced by addition of 10(4)-fold excess of crown gall tumor dna. reconstruction experiments demonstrated that 0.01% added bacterial dna produces a detectable increase in rate of renaturation of labeled dna. crown gall tumor dna therefore cannot contain as much as 0.01% a. tumefaciens dna (one entire bacterial genome per three diploid tumor cells). by the same technique, ps8 bacteriophage dna is not detected in crown gall ...19744530328
quantitative estimation of agrobacterium tumefaciens dna in crown gall tumor cells.several reports suggest that agrobacterium tumefaciens nucleic acids can induce transformation of the cells of susceptible host plants and that bacteria-free tissue cultures of transformed cells contain a. tumefaciens dna, rna, antigens, or bacteriophages. we assayed vinca rosea tumor dna for base sequence homologies with a. tumefaciens dna by dna.dna solution enrichment and dna.dna filter saturation hybridization techniques. no homologies were found by either method. the filter saturation hybri ...19744530329
l-arabinose metabolism in rhizobium japonicum.l-arabinose was metabolized through an oxidative pathway by extracts of a strain of rhizobium japonicum. the findings showed that l-arabinose is converted into 2-keto-3-deoxy-l-arabonate, which is cleaved into glycoaldehyde and pyruvate.19744407017
sensitivity of rhizobia to vanillin. 19744415723
the binding of microsomal hydroxylation substrates to cytochrome p-450rh and its effect on the nitrogen fixation by lupin bacteroids. 19744416102
the effects of antimicrobial substances on rhizobium spp. and their use in selective media. 19744416229
some features of the dna of rhizobium bacteroids and bacteria. 19744417115
characterization of dna from rhizobium cells and their bacteriods from root nodules. 19744417565
large plasmid in agrobacterium tumefaciens essential for crown gall-inducing ability. 19744419109
vitamin b12-dependent methionine synthesis in rhizobium meliloti. 19744422142
plant cell transformation with bacteria. 19744424370
genetic studies on rhizobiophage 16-3. ii. helper-induced transfection. 19744424649
biological control of crown gall: seed and root inoculation. 19744436158
the presence of defective prophages in agrobacterium tumefaciens, strains 806 and b6s. 19744437681
streptomycin resistance in rhizobium japonicum. 19744441216
formation and utilisation of carbon reserves by rhizobium. 19744441218
continuous production of urocanic acid by immobilized achromobacter liquidum cells. 19744441634
studies on the bacteriophage ps-8 of agrobacterium tumefaciens (smith and townsend) conn: purification and properties. 19744451429
[induction of virulent mutants of temperate phages of indicator cultures as a cause of possible erroneous conclusions]. 19744453219
unidirectional growth and branch formation of a morphological mutant, agrobacterium tumefaciens.morphological characteristics of thermoconditional mutant agrobacterium tumefaciens f-502 were investigated in relation to growth, division, and synthesis of cellular components. as a result of a shift from 27 to 37 c, mutant cells altered their morphology from short rods to elongated and branched forms; in addition, division and deoxyribonucleic acid synthesis were inhibited at 37 c. at 37 c unidirectional cell growth and branch formation occurred at one end of a cell, and the elongation rate o ...19744455684
proceedings: investigation of the nucleoside metabolism in rhizobium lupini: obligatory thymidine phosphorylase deficiency. 19744461554
[photosynthesis in lupin leaves in relation to the effectiveness of symbiosis between plant and nodule bacteria]. 19744461738
the nucleoside triphosphate-ribonucleic acid nucleotidyltransferase (ec 2.7.7.6) of agrobacterium tumefaciens (smith and townsend) conn. purification and properties of the enzyme from the tumorigenic strain b6806.the rna nucleotidyltransferase (rna polymerase) of the plant-tumorigenic bacterium agrobacterium tumefaciens was purified. the method involves the disruption of the bacterial cells with glass beads in a waring blendor, treatment with deae-cellulose, fractionation with (nh(4))(2)so(4), protamine sulphate precipitation, deae-cellulose column chromatography and either glycerol-gradient centrifugation or phosphocellulose chromatography. the subunit structure of the highly purified enzyme is similar ...19744462737
[antibody formation against agrobacterium tumefaciens in patients with various cancers]. 19744463498
physiological comparisons of transformed (crown gall) and nontransformed vinca rosea l. cells. 19744471192
regulation of amino acid and glucose dissimilation in so-called ammonifiers and in other soil microorganisms. 19744474858
transformation of effectiveness in rhizobium japonicum. 19744477931
induced variations in microorganisms. v. genetic transformation in rhizobium. 19744479371
effect of certain fungicides on some rhizobia-legume-symbiotic systems. 19744479377
vertical movement of rhizobia in soil. 19744479490
promiscuity in groundnut rhizobium association. 19744479503
effect of some pelleting agents and sodium humate as spray on dry matter yield and nutrient uptake of soybean (glycine max l. cv bragg) in a saline-sodic soil. 19744480492
some studies on the groundnut rhizobium symbiosis. 19744480493
nodulation of soybean under field conditions. 19744480504
host-bacteriophage interaction in agrobacterium tumefaciens. 3. phage-coded endolysins.endolysins were detected in a sensitive strain of agrobacterium tumefaciens (b6) after infection with phage lv-1 and in the lysogen a. tumefaciens v-1 after induction with mitomycin c. a similar endolysin was found in mitomycin c-induced a. tumefaciens c-58, which apparently harbors a defective prophage.19744821492
host-phage interaction in agrobacterium tumefaciens. ii. host strain response to mitomycin c induction. 19744822054
genetic transfer of nitrogen fixation from rhizobium trifolii to klebsiella aerogenes. 19744828191
fast- and slow-growing rhizobia: differences in sucrose utilization and invertase activity. 19744828871
distribution of the isopropylmalate pathway to leucine among diverse bacteria.alpha-isopropylmalate synthase and beta-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: chromatium d, rhodopseudomonas spheroides, hydrogenomonas h16, pseudomonas aeruginosa, pseudomonas fluorescens, vibrio extorquens, rhizobium japonicum, alcaligenes viscolactis, escherichia coli b, proteus vulgaris, aerobacter aerogenes, salmonella typhimurium, micrococcus sp., micrococcus lysodeikticus, bacillus polymyxa, bacillus subtilis, and nocardia opaca. th ...19744829932
ultrastructure of root nodules formed by ineffective strains of rhizobium meliloti. 19744832257
[relation between the content of poly-beta-hydroxybutyric acid in rhizobium lupini bacteroids, their respiration and nitrogen fixation]. 19744833573
letter: the genesis of tumours. 19744834336
[detection of cytokinins in intracellular fractions of purine compounds in rhizobium meliloti dabgeard]. 19744836693
[study of rhizobium lupini polysaccharides in pure culture and in symbiosis]. 19744837341
typing of rhizobium meliloti mutants by means of phages. 19744838507
[effect of certain azomethines on phyopathogenic bacteria]. 19744840336
[spectral properties of cytochrome p-450 from lupin nodule bacteroids]. 19744843933
[effect of substrates and microsomal hydroxylation inhibitors on the nitrogen-fixing activity of lupin bacteroids]. 19744843941
sodium chloride as a cause of low numbers of rhizobium in legume inoculants. 19744846742
[study of sugars and carbohydrate metabolism enzymes in rhizobium lupini in symbiosis]. 19744849805
attempts to detect deoxyribonucleic acid from agrobacterium tumefaciens and bacteriophage ps8 in crown gall tumors by complementary ribonucleic acid-deoxyribonucleic acid-filter hybridization.labeled ribonucleic acid (rna) complementary to agrobacterium tumefaciens dna and ps8 bacteriophage dna (crna) were used in a systematic study of the sensitivity of crna/deoxyribonucleic acid (dna)-filter hybridization for detection of small amounts of phage or bacterial dna immobilized on filters. a. tumefaciens crna of specific activity 10(6) to 2 x 10(6) counts per min per mug reacted to a significant extent when the dna-filter contained 1% a. tumefaciens dna in a salmon dna background, but 0 ...19744850689
dna homology, redundancy and genome size of four rhizobium bacteriophages. 19744851465
fatty acids present in the lipopolysaccharide of rhizobium trifolii.approximately 70% of the fatty acids recovered after acid or alkaline hydrolysis of the lipopolysaccharide of rhizobium trifolii were hydroxy fatty acids identified as hydroxymyristic and hydroxypalmitic acids. palmitic acid was the only saturated fatty acid found in the lipopolysaccharide of r. trifolii. octadecenoic and a small amount of hexadecenoic acids were also identified. the results of bf(3) methanolysis and hydroxylaminolysis suggest that hydroxypalmitic acid is n-acyl bound.19744852028
relation between phage sensitivity and symbiotic properties of auxotrophic mutants of rhizobium meliloti. 19744854080
relationship between the membrane envelope of rhizobial bacteroids and the plasma membrane of the host cell as demonstrated by histochemical localization of adenyl cyclase.by using adenyl cyclase as a marker enzyme, the relationship between the membrane envelope of the bacteroids of rhizobia and the plasma membrane of the host cell was demonstrated histochemically. electron-dense deposits were found on the outer surface of the plasma membrane of the host cell and on the inner surface of the membrane envelopes of the bacteroids, but not in vacuole membranes, endoplasmic reticula, golgi apparatus, and mitochondrial membranes. the results suggest that the membrane en ...19744854087
supercoiled circular dna in crown-gall inducing agrobacterium strains. 19744854526
effects of concentration of volatile metabolites from bacteria and germinating seeds on fungi in the presence of selective absorbents. 19744207172
[8-methoxypsoralen makes agrobacterium tumefaciens, kalanchoe daigremontiana, and the tumor induction sensitive for long wave uv (author's transl)]. 19744277973
[study of the population dynamics of rhizobium leguminosarum in soils using immunofluorescence]. 19744280495
synthesis and resolution of vinylglycine, a beta,gamma-unsaturated alpha-amino acid. 19744212379
[ultrastructure of some bacteriophages of agrobacterium tumefaciens]. 19744214287
legume root nodule nitrogenase. purification, properties, and studies on its genetic control. 19744215454
[prevalence among microorganisms of the ability to synthesize cytokinins]. 19744218857
[regulation of chromosomal transfer in rhizobium lupini]. 19744154658
[sequential mutagenesis in rhizobium lupini]. 19744154660
[genetic studies of class p resistance factors in rhizobium lupini]. 19744154661
[spontaneous induction of a defective rhizobium lupini prophage]. 19744154662
[characterization of a newly isolated rhizobium lupini bacteriophage with a circularizing chromosome]. 19744154663
fluorometric properties of the bibenzimidazole derivative hoechst 33258, a fluorescent probe specific for at concentration in chromosomal dna. 19744136742
[morphological basis of a possible mode of transfer for dna during the conjugation of star-forming soil bacteria (author's transl)]. 19744138745
[ultrastructural and cytochemical studies of bacteroid differentiation of rhizobium trifolii dangeard in the nodules of trifolium repens l]. 19744138777
[occurrence of defective lysogeny in rhizobium lupini]. 19744139842
[insensitivity of defective lysogenic rhizobium lupini strains to homologous phage-like bacteriocins]. 19744139843
comparison of dna polymerase of rhizobium meliloti and alfalfa bacteroids.dna dependent-dna polymerase activity was established and partially purified from extracts of cultured rhizobium meliloti, f-28, and nodule bacteroids (r. meliloti, f-28) of alfalfa plants (medicago sativa). polymerase activity in the partially purified fractions showed characteristic dependence on mg(2+), dna, and a full complement of deoxyribonucleoside triphosphates. dnase activity, preference of "activated" double strand dna, and inhibition by p-chloromercuribenzoate and mncl(2) were respons ...197516659337
attachment of a long-tailed rhizobium bacteriophage to the pili of its host.bacterial strain 16-12 was isolated from the root nodules of lupines and was found to be mitomycin c-inducible for the production of a bacteriophage ("16-12-1") with a long noncontractile tail. the phage was found to attach with a fork-like terminal tail structure to the pili of strain 16-12. in addition, it was also found adsorbed to the bacterial cell poles. it is suggested that phage 16-12-1 may be pilus dependent.197516789164
neutral lipids and phospholipids of free-living and bacteroid forms of two strains of rhizobium infective on lotus pedunculatus.the neutral lipids and phospholipids of two strains of rhizobia in their free-living state and in symbiosis with a host plant are described. the principal lipid classes found were the polymer poly-beta-hydroxybutyrate, phospholipids, free fatty acids, glycerides, methyl esters, aliphatic alcohols, and hydrocarbons. the lipids include unusual unsaturated methyl-branched and saturated methoxy-branched fatty acids. most components were found to be common to both forms of both strains, although the ...197516350025
fructose 1,6-bisphosphate aldolase activity of rhizobium species.fdp aldolase was found to be present in the cell-free extracts of rhizobium leguminosarum, rhizobium phaseoli, rhizobium trifolii, rhizobium meliloti, rhizobium lupini, rhizobium japonicum and rhizobium species from arachis hypogaea and sesbania cannabina. the enzyme in 3 representative species has optimal activity at ph 8.4 in 0.2m veronal buffer. the enzyme activity was completely lost by treatment at 60 degrees c for 15 min. the km values were in the range from 2.38 to 4.55 x 10(-6)m fdp. met ...1975283
seed pelleting with systemic insecticide (aldicarb) for the control of pests of mungo (phaseolus aureus l.). 1975869
properties of some bacteriocins produced by rhizobium trifolii.bacteriocins produced by six strains of rhizobium trifolii were found to be of the relatively low molecular weight, non-phage type. the molecular weights ranged from approximately 1-8 x 105 to 2-0 x 105. all were of protein composition, as indicated by buoyant density (1-32 to 1-34 g/cm3) in csc1 and by sensitivity to proteolytic enzymes. they were resistant to rnaase but sensitive to dnaase. the six bacteriocins could further be separated into two subgroups on the basis of sensitivity to extrem ...19751463
influence of treflan, lindane, and ceresan on different parameters of symbiotic nitrogen fixation and yield in cicer arietinum. 197556828
a general method for the isolation of non-sense suppressor strains in gram-negative bacteria. 197558594
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