Publications

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production of high-viscosity whey-glucose broths by a xanthomonas campestris strain.crude broth viscosities, as high as 700 centipoise at a 12-s shear rate, were produced by fermenting a whey-glucose medium with a xanthomonas campestris strain.198616346971
antagonism of lactic acid bacteria against phytopathogenic bacteria.a variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens xanthomonas campestris, erwinia carotovora, and pseudomonas syringae. effective "in vitro" inhibition was found both on agar plates and in broth cultures. in pot trials, treatment of bean plants with a lactobacillus plantarum strain before inoculation with p. syringae caused a significant reduction of the disease incidence.198616347150
comparison of bacterial lipopolysaccharides by high-performance liquid chromatography.a comparison of lipid-free polysaccharides from gram-negative bacteria was rapidly accomplished by using high-performance liquid chromatography of underivatized hydrolysates. examination of a number of such products revealed that, contrary to earlier reports, xanthomonas campestris lipopolysaccharide contained heptose, together with rhamnose and galactose, but not mannose. the polymers from the methanotrophs "methylomonas albus" and "methylosinus trichosporium" contained heptose and glucose, and ...198616347189
gene-for-gene interactions of five cloned avirulence genes from xanthomonas campestris pv. malvacearum with specific resistance genes in cotton.a total dna clone bank of a strain of xanthomonas campestris pv. malvacearum (xcm) was constructed in the cosmid vector psa747 and transfected into escherichia coli. the xcm strain carries at least nine identifiable avirulence (a) genes. clones in e. coli were mated individually into a recombination-proficient xcm isolate carrying no known a genes. screening was for incompatibility on congenic cotton host lines that differ by single specific resistance (r) genes. ten different cosmid clones conf ...198616593751
production of high-viscosity whey broths by a lactose-utilizing xanthomonas campestris strain.xanthomonas campestris bb-1l was isolated by enrichment and selection by serial passage in a lactose-minimal medium. when bb-1l was subsequently grown in medium containing only 4% whey and 0.05% yeast extract, the lactose was consumed and broth viscosities greater than 500 cps at a 12 s shear rate were produced. prolonged maintenance in whey resulted in the loss of the ability of bb-1l to produce viscous broths in whey, indicating a reversion to preferential growth on whey protein, like the pare ...198516346946
pressate from peat dewatering as a substrate for bacterial growth.this study considered the possibility of using water expressed during the drying of fuel-grade peat as a substrate for microbial growth. highly humified peat pressed for 2.5 min at 1.96 mpa produced water with a chemical oxygen demand of 690 mg/liter. several biological compounds could be produced by using the organic matter in expressed peat water as a substrate. these included polymers such as chitosan, contained in the cell wall of rhizopus arrhizus, and two extracellular polysaccharides, xan ...198516346834
bacterial leaf spot of zinnia elegans, a new disease in argentina.a bacterial disease of zinnia elegans (compositae), caused by xanthomonas campestris pv. zinniae is recorded for the first time in argentina. the identification of the causal organism was based on disease symptoms, pathogenicity and morphological and physiological characteristics of the cultures isolated from material collected at la plata, province of buenos aires, argentina.19853916667
occurrence of maltase and beta-galactosidase in xanthomonas campestris pv. oryzae. 19853928284
evolutionary implications of features of aromatic amino acid biosynthesis in the genus acinetobacter.key enzymes of aromatic amino acid biosynthesis were examined in the genus acinetobacter. members of this genus belong to a suprafamilial assemblage of gram-negative bacteria (denoted superfamily b) for which a phylogenetic tree based upon oligonucleotide cataloging of 16s rrna exists. since the acinetobacter lineage diverged at an early evolutionary time from other lineages within superfamily b, an examination of aromatic biosynthesis in members of this genus has supplied important clues for th ...19854074072
human serum amyloid p component, a circulating lectin with specificity for the cyclic 4,6-pyruvate acetal of galactose. interactions with various bacteria.serum amyloid p component (sap), a normal plasma glycoprotein, has recently been shown to have ca2+-dependent binding specificity for methyl 4,6-o-(1-carboxyethylidene)-beta-d-galactopyranoside (mo beta dg) [hind, collins, renn, cook, caspi, baltz & pepys (1984) j. exp. med. 159, 1058-1069]. sap was found to bind in vitro to klebsiella rhinoscleromatis, the cell wall of which is known to contain this particular cyclic pyruvate acetal of galactose. sap also bound in similar amounts (approx. 6000 ...19853883985
copper-binding characteristics of exopolymers from a freshwater-sediment bacterium.copper-binding activity by exopolymers from adherent cells of a freshwater-sediment bacterium was demonstrated by a combination of equilibrium dialysis and flameless atomic absorption spectrometry. crude, cell-free exopolymer preparations containing protein and polysaccharide components bound up to 37 nmol of cu per mg (dry weight). a highly purified exopolysaccharide preparation bound up to 253 nmol of cu per mg of carbohydrate. the conditional stability constant for the crude exopolymer-cu com ...19854004217
phytoalexin synthesis in soybean cells: elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mrnas and correlation with phytoalexin accumulation.a glucan elicitor from cell walls of the fungus phytophthora megasperma f. sp. glycinea, a pathogen of soybean (glycine max), induced large and rapid increases in the activities of enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase, and of the flavonoid pathway, acetyl-coa carboxylase and chalcone synthase, in suspension-cultured soybean cells. the changes in phenylalanine ammonia-lyase and chalcone synthase activities were correlated with corresponding changes in the mrn ...19846540068
differentiation between xanthomonas campestris pv. oryzae, xanthomonas campestris pv. oryzicola and the bacterial 'brown blotch' pathogen on rice by numerical analysis of phenotypic features and protein gel electrophoregrams.thirty-five xanthomonas campestris pv. oryzae, fourteen x. campestris pv. oryzicola strains and six 'brown blotch' pathogens of rice, all of different geographical origin, were studied by numerical analysis of 133 phenotype features and gel electrophoregrams of soluble proteins, %g + c determinations and dna:rrna hybridizations. the following conclusions were drawn. (i) the xanthomonas campestris pathovars oryzae and oryzicola display clearly distinct protein patterns on polyacrylamide gels and ...19846084704
clues from xanthomonas campestris about the evolution of aromatic biosynthesis and its regulation.the recent placement of major gram-negative prokaryotes (superfamily b) on a phylogenetic tree (including, e.g., lineages leading to escherichia coli, pseudomonas aeruginosa, and acinetobacter calcoaceticus) has allowed initial insights into the evolution of the biochemical pathway for aromatic amino acid biosynthesis and its regulation to be obtained. within this prokaryote grouping, xanthomonas campestris atcc 12612 (a representative of the group v pseudomonads) has played a key role in facili ...19846152589
mechanism and dynamics of conformational ordering in xanthan polysaccharide.the thermally induced order-disorder transition of xanthan (extracellular bacterial polysaccharide from xanthomonas campestris) has been investigated by optical rotation, differential scanning calorimetry, stopped-flow reaction kinetics and low-angle laser light scattering, and the results have been analysed in terms of zimm -bragg helix-coil transition theory. the reciprocal of the transition midpoint temperature (tm) varies linearly with the logarithm of cation (k+) the salt dependence of tm, ...19846726814
genetic construction of lactose-utilizing xanthomonas campestris.xanthomonas campestris, the producer of xanthan gum, possesses a beta-galactosidase of very low specific activity. plasmid pgc9114 (rp1::tn951), generated by the transposition of the lactose transposon tn951 to rp1, was conjugally transferred into xn1, a nalidixic acid-resistant derivative of x. campestris nrrl b-1459s-4l. transfer occurred on membrane filters and in broth. the beta-galactosidase gene of tn951 was expressed in x. campestris. the specific activity of beta-galactosidase in transco ...198416346464
cloning of genes involved in pathogenicity of xanthomonas campestris pv. campestris using the broad host range cosmid plafr1.a genomic library was prepared in escherichia coli from dna of wild-type xanthomonas campestris pv. campestris (aetiological agent of crucifer black rot), partially digested with endonuclease ecori, using the mobilisable broad host range cosmid vector plafr1. recombinant plasmids contained inserts ranging in size from 19.1 to 32.3 kb (mean 26.6). certain of the clones complemented e. coli auxotrophic markers. using the narrow host range plasmid prk2013 as a helper the pooled recombinant plasmids ...198416453595
[transfer of plasmid rp4 into some phytopathogenic bacteria and its relation to their virulence].plasmid rp4 were transferred from escherichia coli into pseudomonas solanacerum, xanthomonas campestris pv. oryzae, x. campestris pv. campestris, and x. campestris pv. citri at the frequencies of 1.8 x 10(-6), 2.8 x 10(-6), 1.4 x 10(-2) and 2.0 x 10(-3), respectively. the frequencies of transfer depended on bacterial species and conjugation conditions. treatment of bromide at the concentration of 2 micrograms/ml and acridine orange at 100 micrograms/ml for 32 hrs the plasmid rp4 could be cured f ...19836342991
immune responses to xanthan gum. i. the characteristics of lymphocyte activation by xanthan gum.xanthan gum (xg), a microbial polysaccharide produced extracellularly by fermentation of xanthomonas campestris, has unique physical properties. we studied the effects of xg on murine lymphocytes in vitro and found that xg induced both a significant increase of dna synthesis in mouse splenic b cells and thymocytes as well as polyclonal igm and igg antibody responses in b cells. xg-activated thymocytes, however, did not display helper or suppressor functions. xg was almost as effective in inducin ...19836832212
the similarities between pseudomonas paucimobilis and allied bacteria derived from analysis of deoxyribonucleic acids and electrophoretic protein patterns.the chromosomal dna was isolated and purified from 17 strains of pseudomonas paucimobilis, and from the type or reference strains of flavobacterium capsulatum, f. devorans, f. multivorum, 'chromobacterium lividum', xanthomonas campestris and seven species of pseudomonas. the dna base compositions (mol% g + c) of p. paucimobilis strains were between 62.2 and 68.6%, and typical strains had a mean value of 65.3 +/- 0.4 mol%, determined from thermal denaturation temperature. dna-dna molecular hybrid ...19827183747
biodegradation of xanthan gum by bacillus sp.strains tentatively identified as bacillus sp. were isolated from sewage sludge and soil and shown to elaborate extracellular enzymes that degrade the extracellular polysaccharide (xanthan gum, polysaccharide b-1459) of xanthomonas campestris nrrl b-1459. enzyme production by one strain was greatly enhanced when the strain was incubated in a mixed culture. products of degradation were identified as d-glucuronic acid, d-mannose, pyruvylated mannose, 6-o-acetyl d-mannose, and a (1-->4)-linked gluc ...198216346068
hot acidified cupric acetate soaks for eradication of xanthomonas campestris from crucifer seeds.acidified cupric acetate soaks were tested for eradication of xanthomonas campestris from naturally infected crucifer seeds. the pathogen was eradicated from seeds by soaking in 0.5% cupric acetate dissolved in 0.005 n acetic acid for 20 min at 35, 40, 45, and 50 degrees c but not 25 degrees c. moreover, normal bacterial flora of crucifer seeds and the seed-borne phoma lingam and alternaria spp. were reduced by 95, 92, and 81%, respectively, after the cupric acetate treatment at 40 degrees c. th ...198016345544
growth and accumulation of polysaccharide by xanthomonas campestris. 19807424048
nutritional studies on xanthan production by xanthomonas campestris nrrl b1459.the nutritional requirements of xanthomonas campestris nrrl b1459 for optimal xanthan production were studied in a chemically defined medium. of the carbon sources tested, a 4% sucrose or glucose medium yielded the highest xanthan titers. the further addition of certain organic acids, such as succinate, pyruvate, and alpha-ketoglutarate, stimulated xanthan production; excess concentrations of these organic acids inhibited xanthan formation. certain amino acids (e.g., glutamate) and nitrate salts ...197916345398
general properties of beta-galactosidase of xanthomonas campestris.partially purified beta-galactosidase of xanthomonas campestris required 32 to 37 degrees c and ph 5.5 to 5.8 for optimum activity. the enzyme had low affinity for lactose hydrolysis (k(m) = 22 mm) and was inhibited by thiol group reagents, ethylenediaminetetraacetic acid, galactose, and d-galactal.197916345439
combining site specificity of antipneumococcal type viii horse immunoglobulins cross-reactive with mild acid-treated xanthomonas campestris polysaccharide. 197828090
maintenance procedures for the curtailment of genetic instability: xanthomonas campestris nrrl b-1459.characteristics are described of small-colony variants of xanthomonas campestris nrrl b-1459 which are frequently encountered when routine culture maintenance procedures are employed. in contrast to the parental type, smallcolony variants were shown to be resistant to a number of antibiotics, to acridine orange, and to phage which are virulent for the parent colony type. sensitivity to ultraviolet radiation was similar in both colony types. a simple method for preservation of viable cells is des ...1977326188
antagonistic interactions of phylloplane bacteria with drechslera dictyoides (drechslera) shoemaker.strains of listeria denitrificans (e2), pseudomonas fluorescens (c37 and c92), and xanthomonas campestris (d119), isolated from the phylloplane of lolium perenne (s24), were antagonistic to drechslera dictyoides (drechslera) shoemaker. from in vitro and in vivo experiments it was deduced that their mode of activity included an initial inhibition of spore germination, a retardation in the rate of germ-tube elongation, and ultimately lysis of the hyphae. the effects were expressed on the plant in ...1977406025
further evidence for the regulation of bacterial populations in soil by protozoa.after the addition to soil of large numbers of a cowpea rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. when indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall initially, but its abundance declined to about 10(7)/g when actidione-resistant protozoa arose in significant numbers. the addition to actidione-treated soil of an antibiotic-resista ...1977879960
bacteriolysis by immobilized enzymes.bacteriolytic enzymes produced by achromobacter lunatus were immobilized in collagen membrane. intact bacteria such as pseudomonas solanacearum, xanthomonas oryzae, staphylococcus aureus, and pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme-collagen membrane. relative activity of the bacteriolytic enzyme-collagen membrane against pseu. solanacearum was about 2% of that of native bacteriolytic enzymes. no difference in the optimum ph was observed between immobilized enzymes and nat ...197714747
[nitrogen utilization and xanthan production by xanthomonas campestris].in media with mixed nitrogen sources (nitrate plus yeast extract) a three auxic growth is observed. the first growth phase is characterized by preferential utilization of the amino acids of the yeast autolysate and the utilization of only small amounts of nitrate. during the second growth phase nitrate is preferentially utilized. in the third phase there is only growth without dividing of cells and the accumulation of xanthan takes place. the change from growth by dividing to growth without divi ...1977930119
colonial variation in xanthomonas campestris nrrl b-1459 and characterization of the polysaccharide from a variant strain.stock cultures of xanthomonas campestris nrrl b-1459 require special attention to maintenance and propagation to assure consistent production in good yields of the extracellular polysaccharide xanthan. under customary conditions of propagative maintenance on agar slants, variant colony types develop that are smaller in size than the normal type. the rate of regression of the normal to the variant forms was diminished when the d-glucose content of the stock medium was sufficient to avoid depletio ...1976963616
covalent structure of the extracellular polysaccharide from xanthomonas campestris: evidence from partial hydrolysis studies. 19761260790
n-nitrosamine formation by cultures of several microorganisms.of 38 pure cultures of microorganisms tested, only one, pseudomonas stutzeri, was capable of forming dimethylnitrosamine from dimethylamine and nitrite during growth. resting cells of p. stutzeri, cryptococcus terreus, escherichia coli, and xanthomonas campestris formed dimethylnitrosamine, although no nitrosamine was found in growing cultures of the latter three organisms. no nitrosamine was produced by either growing cultures or resting-cell suspensions of pseudomonas fragi or proteus mirabili ...19767197
conformation of the extracellular polysaccharide of xanthomonas campestris.the solution conformation of the extracellular polysaccharide of the bacterium xanthomonas campestris is examined by optical rotation, viscometry, and potentiometric titration. measurements of optical rotation vs. temperature for solutions of the polysaccharide at low ionic strength reveal a sharp transition to a denatured structure which is reversible if sufficient salt is present. the temperature tm at the transition midpoint increases as log (na+) or log (ca2+). viscosity-temperature profiles ...19769135
structure of extracellular polysaccharide from xanthomonas campestris. 19751212669
protozoa as agents responsible for the decline of xanthomonas campestris in soil.a streptomycin-resistant mutant of xanthomonas campestris was used to assess the persistence of the plant pathogen in soil and the changes in populations that might be important for its survival. in soil into which large numbers of the organism were introduced, a marked decline in its abundance occurred, but after about 1 week its population density reached a level of about 105 and did not continue to fall during the test period. no such marked decline was evident in sterile soil inoculated wit ...19751115496
growth of xanthomonas campestris is not simply nutrient-limited. 19715156942
biological assays for two mycotoxins produced by fusarium tricinctum.a survey was made to detect microorganisms useful for assaying butenolide [4-acetamido-4-hydroxy-2-butenoic acid gamma-lactone] and t-2 toxin [4beta, 15-diacetoxy-8alpha-(3-methylbutyryloxy)-12,13-epoxytricothec -9-en-3alpha-ol]. these mycotoxins produced by strains of fusarium tricinctum have been implicated in mycotoxicosis of livestock. although butenolide proved to be a very weak antibiotic, assay discs containing 100 mug of this toxin inhibited sprillum serpens nrrl b-2052, vibrio tyrogenus ...19705485724
outbreak of black rot of cabbage (xanthomonas campestris) in udaipur, rajasthan and effect of antibiotics on the growth of the pathogen in vitro. 19695823583
isolation of d-galacturonic acid 1-phosphate from hydrolysates of cell wall lipopolysaccharide extracted from xanthomonas campestris.d-galacturonic acid 1-phosphate was found to be one of the products formed during hydrolysis of the cell wall lipopolysaccharide of xanthomonas campestris in 0.01 n acetic acid at ph 3.3. the molecule was shown to consist of equimolar amounts of d-galacturonic acid and phosphate. resistance to borohydride reduction before, but not after, treatment with escherichia coli alkaline phosphatase indicated that the phosphate group is attached to carbon-1 of the galacturonic acid. the presence of an add ...19684868363
polygalacturonic acid trans-eliminase of xanthomonas campestris.polygalacturonic acid trans-eliminase from the culture fluid of xanthomonas campestris was purified 66-fold by acetone precipitation, citrate extraction and chromatography on diethylaminoethyl- and carboxymethyl-cellulose. the optimum ph is 9.5 in glycine-sodium hydroxide buffer. up to 1mm-calcium chloride brings about a remarkable stimulation of the enzyme activity and, at this concentration, no other cations promote or inhibit enzyme action except ba(2+) ions, which cause complete inhibition. ...19676035509
isolation of a bacterial lipopolysaccharide from xanthomonas campestris containing 3-acetamido-3,6-dideoxy-d-galactose and d-rhamnose. 19665935353
isolation and identification of n-acetyl-3-amino-3,6-dideoxy-d-galactose, a cell wall constituent of xanthomonas campestris. 19655846977
similarity of xanthomonas and pseudomonas deoxyribonucleic acid.de ley, j. (state university, ghent, belgium), and s. friedman. similarity of xanthomonas and pseudomonas deoxyribonucleic acid. j. bacteriol. 89:1306-1309. 1965.-pronounced deoxyribonucleic acid (dna) hybridization was detected with the dna-agar method within the group of xanthomonas and between xanthomonas and pseudomonas. sheared, denatured c(14)-dna from x. pelargonii hybridized 80 to 100% with high molecular weight, denatured agar-bound dna from eight different xanthomonas nomenspecies. the ...196514293002
[on the development of resistance in erwinia carotovora (jones) holland and xanthomonas campestris (pammel) dowson to thiosulfate ethers]. 196314048461
[changes of some biological properties of xanthomonas campestris under the influence of thiosulfonic acid esters. ii. change of the virulence of x. campestris]. 196214460453
[changes in some biological properties of xanthomonas campestris under the influence of thiosulfonic acid esters. i. changes in morphological, biochemical and serological properties of x. campestris]. 196114460452
[on the biological properties of the pathogen of cabbage bacteriosis xanthomonas campestris]. 196113755078
serological studies of the genus xanthomonas: iii. the xanthomonas vascularum and xanthomonas phaseoli groups; the intermediate position of xanthomonas campestris. 194716561367
serological studies of the genus xanthomonas: the xanthomonas vascularum and xanthomonas phaseoli groups: the intermediate position of xanthomonas campestris. 194720266337
vitality of pseudomonas campestris (pam.) smith on cabbage seed. 190417791993
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