| a 54-kda protein encoded by pbtoxis is required for parasporal body structural integrity in bacillus thuringiensis subsp. israelensis. | strains of bacillus thuringiensis such as b. thuringiensis subsp. israelensis (onr-60a) and b. thuringiensis subsp. morrisoni (pg-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions bound together by a multilamellar fibrous matrix. most studies of these strains focus on the molecular biology of the endotoxins, and although it is known that parasporal body stru ... | 2011 | 22210770 |
| surveillance of single-cell behavior in different subpopulations of ralstonia pickettii ar1 during growth and polyhydroxybutyrate production phases by flow cytometry. | most bacterial strains accumulate intracellular polyhydroxybutyrate (phb) granules as an energy reservoir, in response to fluctuations in their microenvironment. flow cytometry was applied for the analysis of single cells of ralstonia pickettii ar1 in response to changes in the culture conditions. two parameters, the phb production-related fl2 and side scatter (ssc) parameters, were used to monitor, distinguish and characterize different subpopulations in the growth and phb production phases. a ... | 2011 | 21780145 |
| metagenomic analysis of taxa associated with lutzomyia longipalpis, vector of visceral leishmaniasis, using an unbiased high-throughput approach. | leishmaniasis is one of the most diverse and complex of all vector-borne diseases worldwide. it is caused by parasites of the genus leishmania, obligate intramacrophage protists characterised by diversity and complexity. its most severe form is visceral leishmaniasis (vl), a systemic disease that is fatal if left untreated. in latin america vl is caused by leishmania infantum chagasi and transmitted by lutzomyia longipalpis. this phlebotomine sandfly is only found in the new world, from mexico t ... | 2011 | 21909446 |
| Complete Genome Sequence of the BTEX-Degrading Bacterium Pseudoxanthomonas spadix BD-a59. | Pseudoxanthomonas spadix BD-a59, able to metabolize all six BTEX (benzene, toluene, ethylbenzene, and o-, m-, and p-xylene) compounds, was isolated from gasoline-contaminated sediment. Here, we report the complete 3.45-Mb genome sequence and annotation of strain BD-a59. These advance the understanding of strain BD-a59's genomic properties and pollutant metabolic versatility. | 2012 | 22207748 |
| Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including High-purity Water. Diversity in Ralstonia pickettii. | Ralstonia pickettii is a nosocomial infectious agent and a significant industrial contaminant. It has been found in many different environments including clinical situations, soil and industrial High Purity Water. This study compares the phenotypic and genotypic diversity of a selection of strains of Ralstonia collected from a variety of sources. | 2011 | 21878094 |
| environmental adaptability and stress tolerance of laribacter hongkongensis: a genome-wide analysis. | | 2011 | 21711489 |
| mechanisms and evolution of oxidative sulfur metabolism in green sulfur bacteria. | green sulfur bacteria (gsb) constitute a closely related group of photoautotrophic and thiotrophic bacteria with limited phenotypic variation. they typically oxidize sulfide and thiosulfate to sulfate with sulfur globules as an intermediate. based on genome sequence information from 15 strains, the distribution and phylogeny of enzymes involved in their oxidative sulfur metabolism was investigated. at least one homolog of sulfide:quinone oxidoreductase (sqr) is present in all strains. in all sul ... | 2011 | 21833341 |
| characterisation of the burkholderia pseudomallei k96243 cps i coding region. | burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic to regions of southeast asia and northern australia. both humans and a range of other animal species are susceptible to melioidosis and the production of a group 3 polysaccharide capsule in b. pseudomallei is essential for virulence. this b. pseudomallei capsular polysaccharide (cps) i comprises unbranched manno-heptopyranose residues and is encoded by a 34.5 kb locus on chromosome 1. despite the importance of thi ... | 2012 | 22252864 |
| Burkholderia species are major inhabitants of white lupin cluster roots. | The formation of cluster roots by plants represents a highly efficient strategy for acquisition of sparingly available phosphate. This particular root type is characterized by a densely branched structure and high exudation of organic acids and protons, which are likely to influence the resident bacterial community. Until now, the identity of the bacterial populations living in cluster roots has not been investigated. We applied cultivation-dependent and cultivation-independent methods to charac ... | 2011 | 21908626 |
| identification and characterization of a novel class of extracellular poly(3-hydroxybutyrate) depolymerase from bacillus sp. strain nrrl b-14911. | the catalytic, linker, and denatured poly(3-hydroxybutyrate) (dphb)-binding domains of bacterial extracellular phb depolymerases (phazs) are classified into several different types. we now report a novel class of extracellular phb depolymerase from bacillus sp. strain nrrl b-14911. its catalytic domain belongs to type 1, whereas its putative linker region neither possesses the sequence features of the three known types of linker domains nor exhibits significant amino acid sequence similarity to ... | 2011 | 21948827 |
| annotation of protein domains reveals remarkable conservation in the functional make up of proteomes across superkingdoms. | the functional repertoire of a cell is largely embodied in its proteome, the collection of proteins encoded in the genome of an organism. the molecular functions of proteins are the direct consequence of their structure and structure can be inferred from sequence using hidden markov models of structural recognition. here we analyze the functional annotation of protein domain structures in almost a thousand sequenced genomes, exploring the functional and structural diversity of proteomes. we find ... | 2011 | 24710297 |
| the active site of tthpolx is adapted to prevent 8-oxo-dgtp misincorporation. | full genome sequencing of bacterial genomes has revealed the presence of numerous genes encoding family x dna polymerases. these enzymes play a variety of biological roles and, accordingly, display often striking functional differences. here we report that the polx from the heat-stable organism thermus thermophilus (tthpolx) inserts the four dntps with strong asymmetry. we demonstrate that this behaviour is related to the presence of a single divergent residue in the active site of tthpolx. muta ... | 2013 | 24084083 |
| the active site of tthpolx is adapted to prevent 8-oxo-dgtp misincorporation. | full genome sequencing of bacterial genomes has revealed the presence of numerous genes encoding family x dna polymerases. these enzymes play a variety of biological roles and, accordingly, display often striking functional differences. here we report that the polx from the heat-stable organism thermus thermophilus (tthpolx) inserts the four dntps with strong asymmetry. we demonstrate that this behaviour is related to the presence of a single divergent residue in the active site of tthpolx. muta ... | 2013 | 24084083 |
| f-/cl- selectivity in clcf-type f-/h+ antiporters. | many bacterial species protect themselves against environmental f(-) toxicity by exporting this anion from the cytoplasm via clc(f) f(-)/h(+) antiporters, a subclass of clc superfamily anion transporters. strong f(-) over cl(-) selectivity is biologically essential for these membrane proteins because cl(-) is orders of magnitude more abundant in the biosphere than f(-). sequence comparisons reveal differences between clc(f)s and canonical cl(-)-transporting clcs within regions that, in the canon ... | 2014 | 25070431 |
| transport genes and chemotaxis in laribacter hongkongensis: a genome-wide analysis. | abstract: | 2011 | 21849034 |
| the quest for biofuels fuels genome sequencing. | | 2008 | 18821974 |
| analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates. | twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ... | 2012 | 23451394 |
| functional genes to assess nitrogen cycling and aromatic hydrocarbon degradation: primers and processing matter. | targeting sequencing to genes involved in key environmental processes, i.e., ecofunctional genes, provides an opportunity to sample nature's gene guilds to greater depth and help link community structure to process-level outcomes. vastly different approaches have been implemented for sequence processing and, ultimately, for taxonomic placement of these gene reads. the overall quality of next generation sequence analysis of functional genes is dependent on multiple steps and assumptions of unknow ... | 2013 | 24062736 |
| a brief review: the z-curve theory and its application in genome analysis. | in theoretical physics, there exist two basic mathematical approaches, algebraic and geometrical methods, which, in most cases, are complementary. in the area of genome sequence analysis, however, algebraic approaches have been widely used, while geometrical approaches have been less explored for a long time. the z-curve theory is a geometrical approach to genome analysis. the z-curve is a three-dimensional curve that represents a given dna sequence in the sense that each can be uniquely reconst ... | 2014 | 24822026 |
| distribution of genes encoding nucleoid-associated protein homologs in plasmids. | bacterial nucleoid-associated proteins (naps) form nucleoprotein complexes and influence the expression of genes. recent studies have shown that some plasmids carry genes encoding nap homologs, which play important roles in transcriptional regulation networks between plasmids and host chromosomes. in this study, we determined the distributions of the well-known naps fis, h-ns, hu, ihf, and lrp and the newly found naps mvat and ndpa among the whole-sequenced 1382 plasmids found in gram-negative b ... | 2011 | 21350637 |
| the prokaryotic antecedents of the ubiquitin-signaling system and the early evolution of ubiquitin-like beta-grasp domains. | ubiquitin (ub)-mediated signaling is one of the hallmarks of all eukaryotes. prokaryotic homologs of ub (this and moad) and e1 ligases have been studied in relation to sulfur incorporation reactions in thiamine and molybdenum/tungsten cofactor biosynthesis. however, there is no evidence for entire protein modification systems with ub-like proteins and deconjugation by deubiquitinating enzymes in prokaryotes. hence, the evolutionary assembly of the eukaryotic ub-signaling apparatus remains unclea ... | 2006 | 16859499 |
| effect of antimicrobial growth promoter administration on the intestinal microbiota of beef cattle. | antimicrobial growth promoters (agps) are antimicrobial agents administered to livestock in feed for prolonged periods to enhance feed efficiency. beef cattle are primarily finished in confined feeding operations in canada and the usa, and the administration of agps such as chlortetracycline and sulfamethazine (aureo s-700 g) is the standard. the impacts of agps on the intestinal microbiota of beef cattle are currently uncertain; it is documented that agps administered to beef cattle pass throug ... | 2013 | 23578222 |
| microbial toluene removal in hypoxic model constructed wetlands occurs predominantly via the ring monooxygenation pathway. | in the present study, microbial toluene degradation in controlled constructed wetland model systems, planted fixed-bed reactors (pfrs), was queried with dna-based methods in combination with stable isotope fractionation analysis and characterization of toluene-degrading microbial isolates. two pfr replicates were operated with toluene as the sole external carbon and electron source for 2 years. the bulk redox conditions in these systems were hypoxic to anoxic. the autochthonous bacterial communi ... | 2015 | 26150458 |
| across bacterial phyla, distantly-related genomes with similar genomic gc content have similar patterns of amino acid usage. | the gc content of bacterial genomes ranges from 16% to 75% and wide ranges of genomic gc content are observed within many bacterial phyla, including both gram negative and gram positive phyla. thus, divergent genomic gc content has evolved repeatedly in widely separated bacterial taxa. since genomic gc content influences codon usage, we examined codon usage patterns and predicted protein amino acid content as a function of genomic gc content within eight different phyla or classes of bacteria. w ... | 2011 | 21423704 |
| the influence of smoking on the peri-implant microbiome. | smokers are at high risk for 2 bacterially driven oral diseases: peri-implant mucositis and peri-implantitis. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the effect of smoking on the peri-implant microbiome in states of health and disease. peri-implant biofilm samples were collected from 80 partially edentulous subjects with peri-implant health, peri-implant mucositis, and peri-implantitis. bacterial dna was isolated and 16s ribsomal rna gene li ... | 2015 | 26124222 |
| effect of modeled reduced gravity conditions on bacterial morphology and physiology. | abstract: background: bacterial phenotypes result from responses to environmental conditions under which these organisms grow; reduced gravity has been demonstrated in many studies as an environmental condition that profoundly influences microorganisms. in this study, we focused on low-shear stress, modeled reduced gravity (mrg) conditions and examined, for escherichia coli and staphlyococcus aureus, a suite of bacterial responses (including total protein concentrations, biovolume, membrane pot ... | 2012 | 22239851 |
| performances of the vitek ms matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology. | rapid and cost-effective matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms)-based systems will replace conventional phenotypic methods for routine identification of bacteria. we report here the first evaluation of the new maldi-tof ms-based vitek ms system in a large clinical microbiology laboratory. this system uses an original spectrum classifier algorithm and a specific database designed for the identification of clinically relevant species. we have te ... | 2012 | 22593596 |
| bacterial chemotaxis toward environmental pollutants: role in bioremediation. | | 2002 | 12450797 |
| growth and multiplexed analysis of microorganisms on a subdivided, highly porous, inorganic chip manufactured from anopore. | a highly porous inorganic material (anopore) was shown to be an effective support for culturing and imaging a wide range of microorganisms. an inert barrier grid was printed on the rigid surface of anopore to create a "living chip" of 336 miniaturized compartments (200/cm2) with broad applications in microbial culture. | 2005 | 16332904 |
| evolution of the oligopeptide transporter family. | the oligopeptide transporter (opt) family of peptide and iron-siderophore transporters includes members from both prokaryotes and eukaryotes but with restricted distribution in the latter domain. eukaryotic members were found only in fungi and plants with a single slime mold homologue clustering with the fungal proteins. all functionally characterized eukaryotic peptide transporters segregate from the known iron-siderophore transporters on a phylogenetic tree. prokaryotic members are widespread, ... | 2011 | 21347612 |
| data correction pre-processing for electronically stored blood culture results: implications on microbial spectrum and empiric antibiotic therapy. | the outcome of patients with bacteraemia is influenced by the initial selection of adequate antimicrobial therapy. the objective of our study was to clarify the influence of different crude data correction methods on a) microbial spectrum and ranking of pathogens, and b) cumulative antimicrobial susceptibility pattern of blood culture isolates obtained from patients from intensive care units (icus) using a computer based tool, moni. | 2009 | 19500418 |
| advances in electronic-nose technologies developed for biomedical applications. | the research and development of new electronic-nose applications in the biomedical field has accelerated at a phenomenal rate over the past 25 years. many innovative e-nose technologies have provided solutions and applications to a wide variety of complex biomedical and healthcare problems. the purposes of this review are to present a comprehensive analysis of past and recent biomedical research findings and developments of electronic-nose sensor technologies, and to identify current and future ... | 2011 | 22346620 |
| hospital epidemiology and infection control in acute-care settings. | health care-associated infections (hais) have become more common as medical care has grown more complex and patients have become more complicated. hais are associated with significant morbidity, mortality, and cost. growing rates of hais alongside evidence suggesting that active surveillance and infection control practices can prevent hais led to the development of hospital epidemiology and infection control programs. the role for infection control programs has grown and continues to grow as rat ... | 2011 | 21233510 |
| structural and thermodynamic characterization of the interaction between two periplasmic treponema pallidum lipoproteins that are components of a tpr-protein-associated trap transporter (tpat). | tripartite atp-independent periplasmic transporters (trap-ts) are bacterial transport systems that have been implicated in the import of small molecules into the cytoplasm. a newly discovered subfamily of trap-ts [tetratricopeptide repeat-protein associated trap transporters (tpats)] has four components. three are common to both trap-ts and tpats: the p component, a ligand-binding protein, and a transmembrane symporter apparatus comprising the m and q components (m and q are sometimes fused to f ... | 2012 | 22504226 |
| guidelines for preventing infectious complications among hematopoietic cell transplantation recipients: a global perspective. | | 2009 | 19747629 |
| biology and pathogenesis of acanthamoeba. | acanthamoeba is a free-living protist pathogen, capable of causing a blinding keratitis and fatal granulomatous encephalitis. the factors that contribute to acanthamoeba infections include parasite biology, genetic diversity, environmental spread and host susceptibility, and are highlighted together with potential therapeutic and preventative measures. the use of acanthamoeba in the study of cellular differentiation mechanisms, motility and phagocytosis, bacterial pathogenesis and evolutionary p ... | 2012 | 22229971 |
| broad-spectrum biosensor capable of detecting and identifying diverse bacterial and candida species in blood. | we describe an assay which uses broad-spectrum, conserved-site pcr paired with mass spectrometry analysis of amplicons (pcr/electrospray ionization-mass spectrometry [esi-ms]) to detect and identify diverse bacterial and candida species in uncultured specimens. the performance of the assay was characterized using whole-blood samples spiked with low titers of 64 bacterial species and 6 candida species representing the breadth of coverage of the assay. the assay had an average limit of detection o ... | 2013 | 23761152 |
| optimized use of the maldi biotyper system and the filmarray bcid panel for direct identification of microbial pathogens from positive blood cultures. | despite the current reliance on blood cultures (bcs), the diagnosis of bloodstream infections (bsis) can be sped up using new technologies performed directly on positive bc bottles. two methods (the maldi biotyper system and filmarray blood culture identification [bcid] panel) are potentially applicable. in this study, we performed a large-scale clinical evaluation (1,585 microorganisms from 1,394 bsi episodes) on the combined use of the maldi biotyper and filmarray bcid panel compared to a refe ... | 2016 | 26677254 |
| rapid 16s rrna next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections. | classifying individual bacterial species comprising complex, polymicrobial patient specimens remains a challenge for culture-based and molecular microbiology techniques in common clinical use. we therefore adapted practices from metagenomics research to rapidly catalog the bacterial composition of clinical specimens directly from patients, without need for prior culture. we have combined a semiconductor deep sequencing protocol that produces reads spanning 16s ribosomal rna gene variable regions ... | 2013 | 23734239 |
| ability of the microscan rapid gram-negative id type 3 panel to identify nonenteric glucose-fermenting and nonfermenting gram-negative bacilli. | the microscan rapid neg id3 panel is designed for the identification of enterobacteriaceae and nonenteric glucose-fermenting and nonfermenting gram-negative bacilli. we evaluated this panel for its ability to identify gram-negative non-enterobacteriaceae bacteria. a total of 134 strains, representing 26 genera and 42 species, were taken from storage at -70(o)c, passaged three times before testing, and inoculated into the panels according to the manufacturer's directions before being inserted int ... | 2002 | 12354875 |
| missing genes in the annotation of prokaryotic genomes. | protein-coding gene detection in prokaryotic genomes is considered a much simpler problem than in intron-containing eukaryotic genomes. however there have been reports that prokaryotic gene finder programs have problems with small genes (either over-predicting or under-predicting). therefore the question arises as to whether current genome annotations have systematically missing, small genes. | 2010 | 20230630 |
| diversity and antibiograms of bacterial organisms isolated from samples of household drinking-water consumed by hiv-positive individuals in rural settings, south africa. | diarrhoea is a hallmark of hiv infections in developing countries, and many diarrhoea-causing agents are often transmitted through water. the objective of the study was to determine the diversity and antibiotic susceptibility profiles of bacterial organisms isolated from samples of household drinking-water consumed by hiv-infected and aids patients. in the present study, household water stored for use by hiv-positive patients was tested for microbial quality, and isolated bacterial organisms wer ... | 2012 | 23082625 |
| epidemiology of bacteremia caused by uncommon non-fermentative gram-negative bacteria. | prevalence of bacteremia caused by non-fermentative gram-negative bacteria (nfgnb) has been increasing over the past decade. although many studies have already investigated epidemiology of nfgnb bacteremia, most focused only on common nfgnb including pseudomonas aeruginosa (pa) and acinetobacter baumannii (ab). knowledge of uncommon nfgnb bacteremia is very limited. our study aimed to investigate epidemiology and identify factors associated with uncommon nfgnb bacteremia. | 2013 | 23566113 |
| microbial redox processes in deep subsurface environments and the potential application of (per)chlorate in oil reservoirs. | the ability of microorganisms to thrive under oxygen-free conditions in subsurface environments relies on the enzymatic reduction of oxidized elements, such as sulfate, ferric iron, or co2, coupled to the oxidation of inorganic or organic compounds. a broad phylogenetic and functional diversity of microorganisms from subsurface environments has been described using isolation-based and advanced molecular ecological techniques. the physiological groups reviewed here comprise iron-, manganese-, and ... | 2014 | 25225493 |
| comprehensive laboratory evaluation of a highly specific lateral flow assay for the presumptive identification of bacillus anthracis spores in suspicious white powders and environmental samples. | we conducted a comprehensive, multiphase laboratory evaluation of the anthrax biothreat alert(®) test strip, a lateral flow immunoassay (lfa) for the rapid detection of bacillus anthracis spores. the study, conducted at 2 sites, evaluated this assay for the detection of spores from the ames and sterne strains of b. anthracis, as well as those from an additional 22 strains. phylogenetic near neighbors, environmental background organisms, white powders, and environmental samples were also tested. ... | 2016 | 27661796 |
| infectious causes of cholesteatoma and treatment of infected ossicles prior to reimplantation by hydrostatic high-pressure inactivation. | chronic inflammation, which is caused by recurrent infections, is one of the factors contributing to the pathogenesis of cholesteatoma. if reimplantation of autologous ossicles after a surgical intervention is intended, inactivation of planktonic bacteria and biofilms is desirable. high hydrostatic pressure treatment is a procedure, which has been used to inactivate cholesteatoma cells on ossicles. here we discuss the potential inactivating effect of high hydrostatic pressure on microbial pathog ... | 2015 | 25705686 |
| real-time comparative evaluation of biomerieux vitek ms versus bruker microflex ms, two matrix-assisted laser desorption-ionization time-of-flight mass spectrometry systems, for identification of clinically significant bacteria. | matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (maldi-tof ms) recently became available for the identification of bacteria in routine diagnostic laboratories. it is rapid and cost-effective and likely to replace phenotypic identification. this study was undertaken to compare two maldi-tof ms-based, bruker microflex ms (bms) and vitek ms (vms) systems, for identification (id) of clinically significant bacterial isolates. clinically relevant broad diversity of bacteri ... | 2014 | 25433488 |
| identification of bacterial protein o-oligosaccharyltransferases and their glycoprotein substrates. | o-glycosylation of proteins in neisseria meningitidis is catalyzed by pgll, which belongs to a protein family including waal o-antigen ligases. we developed two hidden markov models that identify 31 novel candidate pgll homologs in diverse bacterial species, and describe several conserved sequence and structural features. most of these genes are adjacent to possible novel target proteins for glycosylation. we show that in the general glycosylation system of n. meningitidis, efficient glycosylati ... | 2013 | 23658772 |
| redox-coupled structural changes in nitrite reductase revealed by serial femtosecond and microfocus crystallography. | serial femtosecond crystallography (sfx) has enabled the damage-free structural determination of metalloenzymes and filled the gaps of our knowledge between crystallographic and spectroscopic data. crystallographers, however, scarcely know whether the rising technique provides truly new structural insights into mechanisms of metalloenzymes partly because of limited resolutions. copper nitrite reductase (cunir), which converts nitrite to nitric oxide in denitrification, has been extensively studi ... | 2016 | 26769972 |
| structures of protein-protein complexes involved in electron transfer. | electron transfer reactions are essential for life because they underpin oxidative phosphorylation and photosynthesis, processes leading to the generation of atp, and are involved in many reactions of intermediary metabolism. key to these roles is the formation of transient inter-protein electron transfer complexes. the structural basis for the control of specificity between partner proteins is lacking because these weak transient complexes have remained largely intractable for crystallographic ... | 2013 | 23535590 |
| analysis of the sos response of vibrio and other bacteria with multiple chromosomes. | the sos response is a well-known regulatory network present in most bacteria and aimed at addressing dna damage. it has also been linked extensively to stress-induced mutagenesis, virulence and the emergence and dissemination of antibiotic resistance determinants. recently, the sos response has been shown to regulate the activity of integrases in the chromosomal superintegrons of the vibrionaceae, which encompasses a wide range of pathogenic species harboring multiple chromosomes. here we combin ... | 2012 | 22305460 |
| from environment to man: genome evolution and adaptation of human opportunistic bacterial pathogens. | environment is recognized as a huge reservoir for bacterial species and a source of human pathogens. some environmental bacteria have an extraordinary range of activities that include promotion of plant growth or disease, breakdown of pollutants, production of original biomolecules, but also multidrug resistance and human pathogenicity. the versatility of bacterial life-style involves adaptation to various niches. adaptation to both open environment and human specific niches is a major challenge ... | 2012 | 24704914 |
| in vitro activities of bal9141, a novel broad-spectrum pyrrolidinone cephalosporin, against gram-negative nonfermenters. | the activities of bal9141 (formerly ro 63-9141), a novel pyrrolidinone-3-ylidenemethyl cephalosporin, against 244 strains of gram-negative nonfermenters were evaluated. the overall mic at which 50% of isolates are inhibited (mic50) and the overall mic90 were 2 and 64 microg/ml, respectively, which are similar to those of imipenem, lower than those of the other cephalosporins tested, amoxicillin, and the ticarcillin-clavulanic acid combination, and much higher than those of ciprofloxacin. bal9141 ... | 2002 | 11850276 |
| biochemical and susceptibility tests useful for identification of nonfermenting gram-negative rods. | six hundred nineteen strains of nonfermenting gram-negative rods were tested for alkaline phosphatase, benzyl-arginine arylamidase, pyrrolidonyl arylamidase, ethylene glycol acidification, and susceptibility to desferrioxamine and colistin. the results were highly discriminant. therefore, the proposed tests may be helpful for the identification of this group of organisms. | 2002 | 11880447 |
| comparison of traditional phenotypic identification methods with partial 5' 16s rrna gene sequencing for species-level identification of nonfermenting gram-negative bacilli. | correct identification of nonfermenting gram-negative bacilli (nfb) is crucial for patient management. we compared phenotypic identifications of 96 clinical nfb isolates with identifications obtained by 5' 16s rrna gene sequencing. sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify nfb. | 2010 | 20164273 |
| investigation of the bacterial communities associated with females of lutzomyia sand fly species from south america. | phlebotomine sand flies are vectors of leishmania that are acquired by the female sand fly during blood feeding on an infected mammal. leishmania parasites develop exclusively in the gut lumen during their residence in the insect before transmission to a suitable host during the next blood feed. female phlebotomine sand flies are blood feeding insects but their life style of visiting plants as well as animals, and the propensity for larvae to feed on detritus including animal faeces means that t ... | 2012 | 22880020 |
| heavy metal tolerance (cr, ag and hg) in bacteria isolated from sewage. | samples of sewage from a university hospital and a chemistry technical school were analysed for the percentage of bacterial tolerance to chromium (cr), silver (ag) and mercury (hg). additionally, we investigated the effect of these metals on pigmentation and on some enzymatic activities of the metal tolerant strains isolated, as well as antimicrobial resistance in some metal tolerant enterobacteriaceae strains. tolerance to cr was observed mainly in gram positive bacteria while in the case of ag ... | 2012 | 24031994 |
| evaluation of the bruker biotyper and vitek ms matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from cultures from cystic fibrosis patients. | the bruker biotyper and vitek ms matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) instruments were evaluated for the identification of nonfermenting gram-negative bacilli (nfgnb) by a blinded comparison to conventional biochemical or molecular methods. two hundred nfgnb that were recovered from cultures from cystic fibrosis patients in the university of iowa health care (uihc) microbiology laboratory between 1 january 2006 and 31 october 2010 were sen ... | 2012 | 22495566 |
| carbapenem resistance in elizabethkingia meningoseptica is mediated by metallo-β-lactamase blab. | elizabethkingia meningoseptica, a gram-negative rod widely distributed in the environment, is resistant to most β-lactam antibiotics. three bla genes have been identified in e. meningoseptica, coding for the extended-spectrum serine-β-lactamase cme (class d) and two unrelated wide-spectrum metallo-β-lactamases, blab (subclass b1) and gob (subclass b3). e. meningoseptica is singular in being the only reported microorganism possessing two chromosomally encoded mbl genes. real-time pcr and biochemi ... | 2012 | 22290979 |
| marine and giant viruses as indicators of a marine microbial community in a riverine system. | viral communities are important for ecosystem function as they are involved in critical biogeochemical cycles and controlling host abundance. this study investigates riverine viral communities around a small rural town that influences local water inputs. myoviridae, siphoviridae, phycodnaviridae, mimiviridae, herpesviridae, and podoviridae were the most abundant families. viral species upstream and downstream of the town were similar, with synechoccocus phage, salinus, prochlorococcus phage, mim ... | 2016 | 27506856 |
| microscopic, chemical, and molecular-biological investigation of the decayed medieval stained window glasses of two catalonian churches. | we investigated the decayed historical church window glasses of two catalonian churches, both under mediterranean climate. glass surfaces were studied by scanning electron microscopy (sem), energy dispersive spectrometry (eds), and x-ray diffraction (xrd). their chemical composition was determined by wavelength-dispersive spectrometry (wds) microprobe analysis. the biodiversity was investigated by molecular methods: dna extraction from glass, amplification by pcr targeting the16s rrna and its re ... | 2013 | 24092957 |
| discrepancy in maldi-tof ms identification of uncommon gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis. | early identification of microbial organisms from respiratory secretions of patients with cystic fibrosis (cf) is important to guide therapeutic decisions. the objective was to compare the accuracy of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) relative to the conventional phenotypic method in identifying common bacterial isolates, including nonfermenting gram-negative bacteria, in a cohort of patients with cf. | 2015 | 25995646 |
| infectious etiologies of acute febrile illness among patients seeking health care in south-central cambodia. | the agents of human febrile illness can vary by region and country suggesting that diagnosis, treatment, and control programs need to be based on a methodical evaluation of area-specific etiologies. from december 2006 to december 2009, 9,997 individuals presenting with acute febrile illness at nine health care clinics in south-central cambodia were enrolled in a study to elucidate the etiologies. upon enrollment, respiratory specimens, whole blood, and serum were collected. testing was performed ... | 2012 | 22302857 |
| bacteria that travel: the quality of aircraft water. | the travelling population is increasing globally year on year. international tourist arrival figures reached 1087 million in 2013 and 1133 million in 2014; of which 53% and 54% respectively accounted for air transport. the water on board aircraft is sourced from surface or ground water; piped to a central filling point and distributed to each aircraft by water service vehicles at the home base or at the destination airport. the purpose of this study was to ascertain the microbial, chemical (ph; ... | 2015 | 26529000 |
| recent trends in control methods for bacterial wilt diseases caused by ralstonia solanacearum. | previous studies have described the development of control methods against bacterial wilt diseases caused by ralstonia solanacearum. this review focused on recent advances in control measures, such as biological, physical, chemical, cultural, and integral measures, as well as biocontrol efficacy and suppression mechanisms. biological control agents (bcas) have been dominated by bacteria (90%) and fungi (10%). avirulent strains of r. solanacearum, pseudomonas spp., bacillus spp., and streptomyces ... | 2015 | 25762345 |
| the crystal structure of pyrimidine/thiamin biosynthesis precursor-like domain-containing protein cae31940 from proteobacterium bordetella bronchiseptica rb50, and evolutionary insight into the nmt1/thi5 family. | we report a 2.0 å structure of the cae31940 protein, a proteobacterial nmt1/thi5-like domain-containing protein. we also discuss the primary and tertiary structure similarity with its homologs. the highly conserved fggxmp motif was identified in cae31940, which corresponds to the gcccx motif located in the vicinity of the active center characteristic for thi5-like proteins found in yeast. this suggests that the fggxmp motif may be a unique hallmark of proteobacterial nmt1/thi5-like proteins. | 2014 | 24908050 |
| the legionella pneumophila siderophore legiobactin is a polycarboxylate that is identical in structure to rhizoferrin. | legionella pneumophila, the agent of legionnaires' disease, secretes a siderophore (legiobactin) that promotes bacterial infection of the lung. in past work, we determined that cytoplasmic lbta (from legiobactin gene a) promotes synthesis of legiobactin, inner membrane lbtb aids in export of the siderophore, and outer membrane lbtu and inner membrane lbtc help mediate ferrilegiobactin uptake and assimilation. however, the past studies examined legiobactin contained within bacterial culture super ... | 2015 | 26195554 |
| extracellular lipase and protease production from a model drinking water bacterial community is functionally robust to absence of individual members. | bacteria secrete enzymes into the extracellular space to hydrolyze macromolecules into constituents that can be imported for microbial nutrition. in bacterial communities, these enzymes and their resultant products can be modeled as community property. our goal was to investigate the impact of individual community member absence on the resulting community production of exoenzymes (extracellular enzymes) involved in lipid and protein hydrolysis. our model community contained nine bacteria isolate ... | 2015 | 26599415 |
| detection of bacillus anthracis dna in complex soil and air samples using next-generation sequencing. | bacillus anthracis is the potentially lethal etiologic agent of anthrax disease, and is a significant concern in the realm of biodefense. one of the cornerstones of an effective biodefense strategy is the ability to detect infectious agents with a high degree of sensitivity and specificity in the context of a complex sample background. the nature of the b. anthracis genome, however, renders specific detection difficult, due to close homology with b. cereus and b. thuringiensis. we therefore elec ... | 2013 | 24039948 |
| paenibacillus larvae-directed bacteriophage hb10c2 and its application in american foulbrood-affected honey bee larvae. | paenibacillus larvae is the causative agent of american foulbrood (afb), the most serious honey bee brood bacterial disease. we isolated and characterized p. larvae-directed bacteriophages and developed criteria for safe phage therapy. whole-genome analysis of a highly lytic virus of the family siphoviridae (hb10c2) provided a detailed safety profile and uncovered its lysogenic nature and a putative beta-lactamase-like protein. to rate its antagonistic activity against the pathogens targeted and ... | 2015 | 26048941 |
| bioaugmentation: an emerging strategy of industrial wastewater treatment for reuse and discharge. | a promising long-term and sustainable solution to the growing scarcity of water worldwide is to recycle and reuse wastewater. in wastewater treatment plants, the biodegradation of contaminants or pollutants by harnessing microorganisms present in activated sludge is one of the most important strategies to remove organic contaminants from wastewater. however, this approach has limitations because many pollutants are not efficiently eliminated. to counterbalance the limitations, bioaugmentation ha ... | 2016 | 27571089 |
| the characterization of novel tissue microbiota using an optimized 16s metagenomic sequencing pipeline. | substantial progress in high-throughput metagenomic sequencing methodologies has enabled the characterisation of bacteria from various origins (for example gut and skin). however, the recently-discovered bacterial microbiota present within animal internal tissues has remained unexplored due to technical difficulties associated with these challenging samples. | 2015 | 26544955 |
| hexavalent chromium reduction under fermentative conditions with lactate stimulated native microbial communities. | microbial reduction of toxic hexavalent chromium (cr(vi)) in-situ is a plausible bioremediation strategy in electron-acceptor limited environments. however, higher [cr(vi)] may impose stress on syntrophic communities and impact community structure and function. the study objectives were to understand the impacts of cr(vi) concentrations on community structure and on the cr(vi)-reduction potential of groundwater communities at hanford, wa. steady state continuous flow bioreactors were used to gro ... | 2013 | 24376771 |
| the pathochip, a functional gene array for assessing pathogenic properties of diverse microbial communities. | pathogens present in the environment pose a serious threat to human, plant and animal health as evidenced by recent outbreaks. as many pathogens can survive and proliferate in the environment, it is important to understand their population dynamics and pathogenic potential in the environment. to assess pathogenic potential in diverse habitats, we developed a functional gene array, the pathochip, constructed with key virulence genes related to major virulence factors, such as adherence, colonizat ... | 2013 | 23765101 |
| the changing microbial epidemiology in cystic fibrosis. | infection of the airways remains the primary cause of morbidity and mortality in persons with cystic fibrosis (cf). this review describes salient features of the epidemiologies of microbial species that are involved in respiratory tract infection in cf. the apparently expanding spectrum of species causing infection in cf and recent changes in the incidences and prevalences of infection due to specific bacterial, fungal, and viral species are described. the challenges inherent in tracking and int ... | 2010 | 20375354 |
| novel extracellular phb depolymerase from streptomyces ascomycinicus: phb copolymers degradation in acidic conditions. | the ascomycin-producer strain streptomyces ascomycinicus has been proven to be an extracellular poly(r)-3-hydroxybutyrate (phb) degrader. the fkbu gene, encoding a phb depolymerase (phaz sa ), has been cloned in e. coli and rhodococcus sp. t104 strains for gene expression. gram-positive host rhodococcus sp. t104 was able to produce and secrete to the extracellular medium an active protein form. phaz sa was purified by two hydrophobic interaction chromatographic steps, and afterwards was biochemi ... | 2013 | 23951224 |
| selection of clc, cba, and fcb chlorobenzoate-catabolic genotypes from groundwater and surface waters adjacent to the hyde park, niagara falls, chemical landfill. | the frequency of isolation of three nonhomologous chlorobenzoate catabolic genotypes (clc, cba, and fcb) was determined for 464 isolates from freshwater sediments and groundwater in the vicinity of the hyde park industrial landfill site in the niagara watershed. samples were collected from both contaminated and noncontaminated sites during spring, summer, and fall and enriched at 4, 22, or 32 degrees c with micromolar to millimolar concentrations of chlorobenzoates and 3-chlorobiphenyl (m. c. pe ... | 1999 | 10103260 |
| pulmonary bacterial pathogens in cystic fibrosis patients and antibiotic therapy: a tool for the health workers. | cystic fibrosis is the most common and best known genetic disease involving a defect in transepithelial cl- transport by mutations in the cf gene on chromosome 7, which codes for the cystic fibrosis transmembrane conductance regulator protein (cftr). the most serious symptoms are observed in the lungs, augmenting the risk of bacterial infection. the objective of this review was to describe the bacterial pathogens colonizing patients with cystic fibrosis. a systematic search was conducted using t ... | 2008 | 18992146 |
| spatial patterns in antibiotic resistance among stream bacteria: effects of industrial pollution. | the spatial distribution of antibiotic resistance to streptomycin and kanamycin was examined in natural bacterial communities of two streams. the proportion of resistant bacteria was substantially higher (p < 0.05) in the midreaches of an industrially perturbed stream, but no such pattern was apparent in an undisturbed reference stream. the highest relative frequency of resistance was found at the confluence of a tributary draining a nuclear reactor and industrial complex. antibiotic resistance ... | 2000 | 10966382 |
| spatial changes in the bacterial community structure along a vertical oxygen gradient in flooded paddy soil cores. | molecular ecology techniques were applied to assess changes in the bacterial community structure along a vertical oxygen gradient in flooded paddy soil cores. microsensor measurements showed that oxygen was depleted from 140 microm at the floodwater/soil interface to nondetectable amounts at a depth of approximately 2.0 mm and below. bacterial 16s rrna gene (rdna)-based community fingerprint patterns were obtained from 200-microm-thick soil slices of both the oxic and anoxic zones by using the t ... | 2000 | 10653747 |
| analysis of the bacterial communities present in lungs of patients with cystic fibrosis from american and british centers. | the aim of this study was to determine whether geographical differences impact the composition of bacterial communities present in the airways of cystic fibrosis (cf) patients attending cf centers in the united states or united kingdom. thirty-eight patients were matched on the basis of clinical parameters into 19 pairs comprised of one u.s. and one united kingdom patient. analysis was performed to determine what, if any, bacterial correlates could be identified. two culture-independent strategi ... | 2010 | 21068277 |
| analysis of the bacterial communities present in lungs of patients with cystic fibrosis from american and british centers. | the aim of this study was to determine whether geographical differences impact the composition of bacterial communities present in the airways of cystic fibrosis (cf) patients attending cf centers in the united states or united kingdom. thirty-eight patients were matched on the basis of clinical parameters into 19 pairs comprised of one u.s. and one united kingdom patient. analysis was performed to determine what, if any, bacterial correlates could be identified. two culture-independent strategi ... | 2010 | 21068277 |
| the promise of bacteriophage therapy for burkholderia cepacia complex respiratory infections. | in recent times, increased attention has been given to evaluating the efficacy of phage therapy, especially in scenarios where the bacterial infectious agent of interest is highly antibiotic resistant. in this regard, phage therapy is especially applicable to infections caused by the burkholderia cepacia complex (bcc) since members of the bcc are antibiotic pan-resistant. current studies in bcc phage therapy are unique from many other avenues of phage therapy research in that the investigation i ... | 2011 | 22919592 |
| the promise of bacteriophage therapy for burkholderia cepacia complex respiratory infections. | in recent times, increased attention has been given to evaluating the efficacy of phage therapy, especially in scenarios where the bacterial infectious agent of interest is highly antibiotic resistant. in this regard, phage therapy is especially applicable to infections caused by the burkholderia cepacia complex (bcc) since members of the bcc are antibiotic pan-resistant. current studies in bcc phage therapy are unique from many other avenues of phage therapy research in that the investigation i ... | 2011 | 22919592 |
| rapid identification of acinetobacter spp. by fluorescence in situ hybridization (fish) from colony and blood culture material. | multi-drug-resistant strains of the acinetobacter baumannii complex cause nosocomial infections. rapid identification of acinetobacter spp. is desirable in order to facilitate therapeutic or hygiene decisions. we evaluated a newly designed dna probe that can be used under standard conditions in both a microwave oven and a slide chamber for the rapid identification of acinetobacter spp. by fluorescence in situ hybridization (fish). using fish, the new probe correctly identified 81/81 acinetobacte ... | 2011 | 24516735 |
| defining falciparum-malaria-attributable severe febrile illness in moderate-to-high transmission settings on the basis of plasma pfhrp2 concentration. | in malaria-endemic settings, asymptomatic parasitemia complicates the diagnosis of malaria. histidine-rich protein 2 (hrp2) is produced by plasmodium falciparum, and its plasma concentration reflects the total body parasite burden. we aimed to define the malaria-attributable fraction of severe febrile illness, using the distributions of plasma p. falciparum hrp2 (pfhrp2) concentrations from parasitemic children with different clinical presentations. | 2012 | 23136222 |
| defining falciparum-malaria-attributable severe febrile illness in moderate-to-high transmission settings on the basis of plasma pfhrp2 concentration. | in malaria-endemic settings, asymptomatic parasitemia complicates the diagnosis of malaria. histidine-rich protein 2 (hrp2) is produced by plasmodium falciparum, and its plasma concentration reflects the total body parasite burden. we aimed to define the malaria-attributable fraction of severe febrile illness, using the distributions of plasma p. falciparum hrp2 (pfhrp2) concentrations from parasitemic children with different clinical presentations. | 2012 | 23136222 |
| multidrug-resistance and toxic metal tolerance of medically important bacteria isolated from an aquaculture system. | the use of antimicrobials and toxic metals should be considered carefully in aquaculture and surrounding environments. we aimed to evaluate medically relevant bacteria in an aquaculture system and their susceptibility to antimicrobials and toxic metals. selective cultures for enterobacteria (ent), non-fermenting gram-negative rods (nfr) and gram-positive cocci (gpc) were obtained from water samples collected in two different year seasons. the isolated bacteria were biochemically identified and a ... | 2012 | 22972388 |
| a multilocus sequence typing scheme implies population structure and reveals several putative novel achromobacter species. | the genus achromobacter currently is comprised of seven species, including achromobacter xylosoxidans, an opportunistic and nosocomial pathogen that displays broad-spectrum antimicrobial resistance and is recognized as causing chronic respiratory tract infection in persons with cystic fibrosis (cf). to enable strain typing for global epidemiologic investigations, to clarify the taxonomy of "achromobacter-like" strains, and to elucidate the population structure of this genus, we developed a genus ... | 2012 | 22785192 |
| bacterial contamination of dental unit waterlines. | safety of patients and dental personnel requires the appropriate microbiological water quality in dental units. during treatment, patients and dental workers are exposed both to direct contact with bacteria-contaminated water in the form of splatter and with contaminated water aerosol emitted during work by unit handpieces, including rotating and ultrasonic instruments. the aim of the study was to determine the qualitative and quantitative contamination of water in dental unit reservoirs with ae ... | 2012 | 22899458 |
| bacterial contamination of dental unit waterlines. | safety of patients and dental personnel requires the appropriate microbiological water quality in dental units. during treatment, patients and dental workers are exposed both to direct contact with bacteria-contaminated water in the form of splatter and with contaminated water aerosol emitted during work by unit handpieces, including rotating and ultrasonic instruments. the aim of the study was to determine the qualitative and quantitative contamination of water in dental unit reservoirs with ae ... | 2012 | 22899458 |
| combination antimicrobial susceptibility testing of multidrug-resistant stenotrophomonas maltophilia from cystic fibrosis patients. | stenotrophomonas maltophilia is increasingly being isolated from the respiratory tract of individuals with cystic fibrosis, and, because of its multidrug-resistant nature, the selection of suitable treatment regimens can be problematical. etest methodology was used to facilitate mic and antimicrobial combination testing on 80 isolates of s. maltophilia cultured from the respiratory tract of scottish individuals with cystic fibrosis between 2001 and 2010. the overall rate of susceptibility for th ... | 2012 | 22585220 |
| centromere binding and evolution of chromosomal partition systems in the burkholderiales. | how split genomes arise and evolve in bacteria is poorly understood. since each replicon of such genomes encodes a specific partition (par) system, the evolution of par systems could shed light on their evolution. the cystic fibrosis pathogen burkholderia cenocepacia has three chromosomes (c1, c2, and c3) and one plasmid (pbc), whose compatibility depends on strictly specific interactions of the centromere sequences (pars) with their cognate binding proteins (parb). however, the par systems of b ... | 2012 | 22522899 |
| distribution and properties of the genes encoding the biosynthesis of the bacterial cofactor, pyrroloquinoline quinone. | pyrroloquinoline quinone (pqq) is a small, redox active molecule that serves as a cofactor for several bacterial dehydrogenases, introducing pathways for carbon utilization that confer a growth advantage. early studies had implicated a ribosomally translated peptide as the substrate for pqq production. this study presents a sequence- and structure-based analysis of the components of the pqq operon. we find the necessary components for pqq production are present in 126 prokaryotes, most of which ... | 2012 | 22324760 |
| stable carbon isotope fractionation in chlorinated ethene degradation by bacteria expressing three toluene oxygenases. | one difficulty in using bioremediation at a contaminated site is demonstrating that biodegradation is actually occurring in situ. the stable isotope composition of contaminants may help with this, since they can serve as an indicator of biological activity. to use this approach it is necessary to establish how a particular biodegradation pathway affects the isotopic composition of a contaminant. this study examined bacterial strains expressing three aerobic enzymes for their effect on the (13)c/ ... | 2012 | 22363335 |
| complexity, temporal stability, and clinical correlates of airway bacterial community composition in primary ciliary dyskinesia. | primary ciliary dyskinesia (pcd) is a genetic disease characterized by abnormalities in ciliary function, leading to compromised airway clearance and chronic bacterial infection of the upper and lower airways. the compositions of these infections and the relationships between their characteristics and disease presentation are poorly defined. we describe here the first systematic culture-independent evaluation of lower airway bacteriology in pcd. thirty-three airway samples (26 from sputum, 7 fro ... | 2013 | 24068019 |
| prevent infection linked to the dialysis water in a hemodialysis center in fez city (morocco). | water treatment systems are a critical variable in dialysis therapy. rigorous control of hemodialysis water quality is particularly important in order to guarantee a better quality of life of the hemodialysis patients. the objective of the study was to evaluate the chemical, microbiological quality and antimicrobial resistance of bacteria isolated from water and dialysate in a public hd center. | 2013 | 24839530 |
| the use of platensimycin and platencin to fight antibiotic resistance. | infectious diseases are known as one of the most life-threatening disabilities worldwide. approximately 13 million deaths related to infectious diseases are reported each year. the only way to combat infectious diseases is by chemotherapy using antimicrobial agents and antibiotics. however, due to uncontrolled and unnecessary use of antibiotics in particular, surviving bacteria have evolved resistance against several antibiotics. emergence of multidrug resistance in bacteria over the past severa ... | 2013 | 24082790 |
| evaluation of a new lipase from staphylococcus sp. for detergent additive capability. | lipases are the enzymes of choice for laundry detergent industries owing to their triglyceride removing ability from the soiled fabric which eventually reduces the usage of phosphate-based chemical cleansers in the detergent formulation. in the present study, a partially purified bacterial lipase from staphylococcus arlettae jpbw-1 isolated from the rock salt mine has been assessed for its triglyceride removing ability by developing a presoak solution so as to use lipase as an additive in laundr ... | 2013 | 24106703 |
| copper enhanced monooxygenase activity and ft-ir spectroscopic characterisation of biotransformation products in trichloroethylene degrading bacterium: stenotrophomonas maltophilia pm102. | stenotrophomonas maltophilia pm102 (ncbi genbank acc. no. jq797560) is capable of growth on trichloroethylene as the sole carbon source. in this paper, we report the purification and characterisation of oxygenase present in the pm102 isolate. enzyme activity was found to be induced 10.3-fold in presence of 0.7 mm copper with a further increment to 14.96-fold in presence of 0.05 mm nadh. optimum temperature for oxygenase activity was recorded at 36°c. the reported enzyme was found to have enhance ... | 2013 | 24083236 |