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from community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic bacteroidetes generalists.the microbiota of multi-pond solar salterns around the world has been analyzed using a variety of culture-dependent and molecular techniques. however, studies addressing the dynamic nature of these systems are very scarce. here we have characterized the temporal variation during 1 year of the microbiota of five ponds with increasing salinity (from 18% to >40%), by means of card-fish and dgge. microbial community structure was statistically correlated with several environmental parameters, includ ...201424926861
teleost microbiomes: the state of the art in their characterization, manipulation and importance in aquaculture and fisheries.indigenous microbiota play a critical role in the lives of their vertebrate hosts. in human and mouse models it is increasingly clear that innate and adaptive immunity develop in close concert with the commensal microbiome. furthermore, several aspects of digestion and nutrient metabolism are governed by intestinal microbiota. research on teleosts has responded relatively slowly to the introduction of massively parallel sequencing procedures in microbiomics. nonetheless, progress has been made i ...201424917852
cyanuric acid hydrolase from azorhizobium caulinodans ors 571: crystal structure and insights into a new class of ser-lys dyad proteins.cyanuric acid hydrolase (cah) catalyzes the hydrolytic ring-opening of cyanuric acid (2,4,6-trihydroxy-1,3,5-triazine), an intermediate in s-triazine bacterial degradation and a by-product from disinfection with trichloroisocyanuric acid. in the present study, an x-ray crystal structure of the cah-barbituric acid inhibitor complex from azorhizobium caulinodans ors 571 has been determined at 2.7 å resolution. the cah protein fold consists of three structurally homologous domains forming a β-barre ...201424915109
quorum quenching mediated approaches for control of membrane biofouling.membrane biofouling is widely acknowledged as the most frequent adverse event in wastewater treatment systems resulting in significant loss of treatment efficiency and economy. different strategies including physical cleaning and use of antimicrobial chemicals or antibiotics have been tried for reducing membrane biofouling. such traditional practices are aimed to eradicate biofilms or kill the bacteria involved, but the greater efficacy in membrane performance would be achieved by inhibiting bio ...201424910534
new insights into 1-aminocyclopropane-1-carboxylate (acc) deaminase phylogeny, evolution and ecological significance.the main objective of this work is the study of the phylogeny, evolution and ecological importance of the enzyme 1-aminocyclopropane-1-carboxylate (acc) deaminase, the activity of which represents one of the most important and studied mechanisms used by plant growth-promoting microorganisms. the acc deaminase gene and its regulatory elements presence in completely sequenced organisms was verified by multiple searches in diverse databases, and based on the data obtained a comprehensive analysis w ...201424905353
a novel, nested, multiplex, real-time pcr for detection of bacteria and fungi in blood.the study describes the application of the pcr method for the simultaneous detection of dna of gram-negative bacteria, gram-positive bacteria, yeast fungi and filamentous fungi in blood and, thus, a whole range of microbial etiological agents that may cause sepsis. material for the study was sterile blood inoculated with four species of microorganisms (escherichia coli, staphylococcus aureus, candida albicans and aspergillus fumigatus) and blood collected from patients with clinical symptoms of ...201424893651
biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity.current soil management strategies are mainly dependent on inorganic chemical-based fertilizers, which caused a serious threat to human health and environment. the exploitation of beneficial microbes as a biofertilizer has become paramount importance in agriculture sector for their potential role in food safety and sustainable crop production. the eco-friendly approaches inspire a wide range of application of plant growth promoting rhizobacteria (pgprs), endo- and ectomycorrhizal fungi, cyanobac ...201424885352
the gut microbiota of larvae of rhynchophorus ferrugineus oliver (coleoptera: curculionidae).the red palm weevil (rpw) rhynchophorus ferrugineus olivier (coleoptera: curculionidae) is one of the major pests of palms. the larvae bore into the palm trunk and feed on the palm tender tissues and sap, leading the host tree to death. the gut microbiota of insects plays a remarkable role in the host life and understanding the relationship dynamics between insects and their microbiota may improve the biological control of insect pests. the purpose of this study was to analyse the diversity of t ...201424884866
natural rice rhizospheric microbes suppress rice blast infections.the natural interactions between plant roots and their rhizospheric microbiome are vital to plant fitness, modulating both growth promotion and disease suppression. in rice (oryza sativa), a globally important food crop, as much as 30% of yields are lost due to blast disease caused by fungal pathogen magnaporthe oryzae. capitalizing on the abilities of naturally occurring rice soil bacteria to reduce m. oryzae infections could provide a sustainable solution to reduce the amount of crops lost to ...201424884531
evolving epidemiology and antimicrobial resistance in spontaneous bacterial peritonitis: a two-year observational study.current recommendations for empirical antimicrobial therapy in spontaneous bacterial peritonitis (sbp) are based on quite old trials. since microbial epidemiology and the management of patients have changed, whether these recommendations are still appropriate must be confirmed.201424884471
u2 snrnp is required for expression of the 3' end of genes.pre-mrna in eukaryotes is subjected to mrna processing, which includes capping, polyadenylation, and splicing. transcription and mrna processing are coupled, and this coupling stimulates mrna processing; however, the effects of mrna processing on transcription are not fully understood. in this study, we found that inhibition of u2 snrnp by a splicing inhibitor, spliceostatin a (ssa), or by an antisense oligonucleotide to u2 snrna, caused gene-specific 3'-end down-regulation. removal of ssa from ...201424845214
prevent infection linked to the dialysis water in a hemodialysis center in fez city (morocco).water treatment systems are a critical variable in dialysis therapy. rigorous control of hemodialysis water quality is particularly important in order to guarantee a better quality of life of the hemodialysis patients. the objective of the study was to evaluate the chemical, microbiological quality and antimicrobial resistance of bacteria isolated from water and dialysate in a public hd center.201324839530
inhibition of batrachochytrium dendrobatidis caused by bacteria isolated from the skin of boreal toads, anaxyrus (bufo) boreas boreas, from grand teton national park, wyoming, usa.the chytrid fungus batrachochytrium dendrobatidis is a significant cause of the worldwide decline in amphibian populations; however, various amphibian species are capable of coexisting with b. dendrobatidis. among them are boreal toads (anaxyrus (bufo) boreas boreas) located in grand teton national park (gtnp) in wyoming, usa. the purpose of this study was to identify cultivable bacterial isolates from the skin microbiota of boreal toads from gtnp and determine if they were capable of inhibiting ...201424826077
the dimerization domain in dape enzymes is required for catalysis.the emergence of antibiotic-resistant bacterial strains underscores the importance of identifying new drug targets and developing new antimicrobial compounds. lysine and meso-diaminopimelic acid are essential for protein production and bacterial peptidoglycan cell wall remodeling and are synthesized in bacteria by enzymes encoded within dap operon. therefore dap enzymes may serve as excellent targets for developing a new class of antimicrobial agents. the dape-encoded n-succinyl-l,l-diaminopimel ...201424806882
characterization and function of the first antibiotic isolated from a vent organism: the extremophile metazoan alvinella pompejana.the emblematic hydrothermal worm alvinella pompejana is one of the most thermo tolerant animal known on earth. it relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. here, by studying the pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. after purification and peptide sequencing, both the gene and the peptide tertiary struct ...201424776651
fast and economic immobilization methods described for non-commercial pseudomonas lipases.there is an increasing interest to seek new enzyme preparations for the development of new products derived from bioprocesses to obtain alternative bio-based materials. in this context, four non-commercial lipases from pseudomonas species were prepared, immobilized on different low-cost supports, and examined for potential biotechnological applications.201424755191
food, pathogen, signal: the multifaceted nature of a bacterial diet.c. elegans, both in the wild and in the lab, live on a diet of live bacteria. the bacterial diet provides nutrients for c. elegans, but can also play a number of other roles in c. elegans physiology. recently, we compared the effects of different bacterial diets on life history traits and gene expression. here, we discuss our recent findings in the context of other dietary studies and highlight challenges in understanding dietary effects. for instance, since bacteria can be pathogenic it can be ...201324744980
isolation and characterization of broad spectrum coaggregating bacteria from different water systems for potential use in bioaugmentation.the bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. in this study, through a visual and semi-quantitative assay, twenty-two bacterial strains with aggregation ability were obtained from 8 different water environments, and these strains were assigned to 7 genera according to their 16s rdna and they were aeromonas, bacillus, comamonas, exiguobacterium, pseudomonas, shewanella and comamonas. furthermore, all possible 231 pa ...201424736645
microbial growth and carbon use efficiency in the rhizosphere and root-free soil.plant-microbial interactions alter c and n balance in the rhizosphere and affect the microbial carbon use efficiency (cue)-the fundamental characteristic of microbial metabolism. estimation of cue in microbial hotspots with high dynamics of activity and changes of microbial physiological state from dormancy to activity is a challenge in soil microbiology. we analyzed respiratory activity, microbial dna content and cue by manipulation the c and nutrients availability in the soil under beta vulgar ...201424722409
engineering non-heme mono- and dioxygenases for biocatalysis.oxygenases are ubiquitous enzymes that catalyze the introduction of one or two oxygen atoms to unreactive chemical compounds. they require reduction equivalents from nadh or nadph and comprise metal ions, metal ion complexes, or coenzymes in their active site. thus, for industrial purposes, oxygenases are most commonly employed using whole cell catalysis, to alleviate the need for co-factor regeneration. biotechnological applications include bioremediation, chiral synthesis, biosensors, fine che ...201224688652
classification of healthcare-associated infection: a systematic review 10 years after the first proposal.ten years after the first proposal, a consensus definition of healthcare-associated infection (hcai) has not been reached, preventing the development of specific treatment recommendations. a systematic review of all definitions of hcai used in clinical studies is made.201424597462
characterization of 3-phosphoglycerate kinase from corynebacterium glutamicum and its impact on amino acid production.corynebacterium glutamicum cg1790/pgk encodes an enzyme active as a 3-phosphoglycerate kinase (pgk) (ec 2.7.2.3) catalyzing phosphoryl transfer from 1,3-biphosphoglycerate (bpg) to adp to yield 3-phosphoglycerate (3-pg) and atp in substrate chain phosphorylation.201424593686
antibacterial activity of rhizome of curcuma aromatica and partial purification of active compounds.the hexane extract of curcuma aromatica, a plant belonging to the family zingiberaceae was tested on 10 bacterial strains (clinical isolates and standard strains). agar diffusion method was adopted for determining the antibacterial activity of the extract. the hexane extract was found to be active against all gram-positive strains tested, but inactive against gram-negative strains. the minimum inhibitory concentration and minimum bactericidal concentration were determined and found to be 539 μg/ ...201324591751
bacterial degradation of chlorophenols and their derivatives.chlorophenols (cps) and their derivatives are persistent environmental pollutants which are used in the manufacture of dyes, drugs, pesticides and other industrial products. cps, which include monochlorophenols, polychlorophenols, chloronitrophenols, chloroaminophenols and chloromethylphenols, are highly toxic to living beings due to their carcinogenic, mutagenic and cytotoxic properties. several physico-chemical and biological methods have been used for removal of cps from the environment. bact ...201424589366
cloning and characterisation of (r)-3-hydroxyacyl-acyl carrier protein-coenzyme a transferase gene (phag) from pseudomonas sp. usm 4-55.the (r)-3-hydroxyacyl-acp-coa transferase catalyses the conversion of (r)-3-hydroxyacyl-acp to (r)-3-hydroxyacyl-coa derivatives, which serves as the ultimate precursor for polyhydroxyalkanoate (pha) polymerisation from unrelated substrates in pseudomonads. phag was found to be responsible for channelling precursors for polyhydroxyalkanoate (pha) synthase from a de novo fatty acid biosynthesis pathway when cultured on carbohydrates, such as glucose or gluconate. the phag gene was cloned from pse ...200924575175
structural characterization of a hypothetical protein: a potential agent involved in trimethylamine metabolism in catenulispora acidiphila.catenulispora acidiphila is a newly identified lineage of actinomycetes that produces antimicrobial activities and represents a promising source of novel antibiotics and secondary metabolites. among the discovered protein coding genes, 68 % were assigned a putative function, while the remaining 32 % are genes encoding "hypothetical" proteins. caci_0382 is one of the "hypothetical" proteins that has very few homologs. sequence analysis shows that the protein belongs to the ntf2-like protein famil ...201424562475
postoperative endophthalmitis following cataract surgery in asia.postoperative endophthalmitis is a rare clinical occurrence. however, it remains one of the most serious complications following cataract surgery because of its poor prognosis. we investigated the epidemiologic trends in postoperative endophthalmitis following cataract surgery, particularly in asian populations. the incidence of postcataract endophthalmitis was generally consistent with epidemiologic data reported from caucasian populations. the most frequently occurring causative organism was c ...201124555121
community acquired bacteremia by sphingomonas paucimobilis: two rare case reports.s.paucimobilis has a diverse nutritional substrate spectrum and found in both environmental and hospital settings. sphingomonas paucimobilis is rarely isolated from clinical specimen. this low virulence organism since has been reported to cause a variety of diseases since 1979. it has been reported to be associated with both community acquired and nosocomial diseases including bacteremia, catheter related sepsis, diarrhoeal diseases, peritonitis, meningitis, cutaneous infections, endopthalmitis, ...201324551684
microbial enhanced heavy oil recovery by the aid of inhabitant spore-forming bacteria: an insight review.crude oil is the major source of energy worldwide being exploited as a source of economy, including oman. as the price of crude oil increases and crude oil reserves collapse, exploitation of oil resources in mature reservoirs is essential for meeting future energy demands. as conventional recovery methods currently used have become less efficient for the needs, there is a continuous demand of developing a new technology which helps in the upgradation of heavy crude oil. microbial enhanced oil re ...201424550702
burkholderia ambifaria and b. caribensis promote growth and increase yield in grain amaranth (amaranthus cruentus and a. hypochondriacus) by improving plant nitrogen uptake.grain amaranth is an emerging crop that produces seeds having high quality protein with balanced amino-acid content. however, production is restricted by agronomic limitations that result in yields that are lower than those normally produced by cereals. in this work, the use of five different rhizobacteria were explored as a strategy to promote growth and yields in amaranthus hypochondriacus cv. nutrisol and a. cruentus cv. candil, two commercially important grain amaranth cultivars. the plants ...201424533068
outpatient management following intensive induction or salvage chemotherapy for acute myeloid leukemia.adults with newly diagnosed or relapsed acute myeloid leukemia (aml) commonly receive intensive chemotherapy to achieve disease remission. in the united states and many other countries, it is standard practice that these patients remain hospitalized "preemptively" until blood count recovery, owing to the risk for overwhelming infections and bleeding during pancytopenia. this care policy requires hospitalization for an average of 3 to 4 weeks after completion of chemotherapy. however, highly effe ...201324518520
a novel approach for pathogen reduction in wastewater treatment.many sewage waste treatment systems are aiming for complete pathogen removal which necessitates search for novel approaches that does not harm the environment. one such novel approach is exploring the possibilities of bacteriophages for pathogen removal. hospital wastewater was collected from different locations of tamil nadu and used for the study. the total heterotroph and total coliform population ranged from 1.6 × 105 to 8.3 × 106 per ml and from 1.2 × 103 to 1.6 × 103/ 100 ml of sample resp ...201324499588
atrazine removal from aqueous solutions using submerged biological aerated filter.atrazine is widely used in the agriculture as an herbicide. due to its high mobility, atrazine leaks into the groundwaters, surface waters, and drinking water wells. many physical and chemical methods have been suggested for removing atrazine from aquatic environments. however, these methods are very costly, have many performance problems, produce a lot of toxic intermediates which are very harmful and dangerous, and cannot completely mineralize atrazine. in this study, biodegradation of atrazin ...201324499572
current status on biochemistry and molecular biology of microbial degradation of nicotine.bioremediation is one of the most promising methods to clean up polluted environments using highly efficient potent microbes. microbes with specific enzymes and biochemical pathways are capable of degrading the tobacco alkaloids including highly toxic heterocyclic compound, nicotine. after the metabolic conversion, these nicotinophilic microbes use nicotine as the sole carbon, nitrogen, and energy source for their growth. various nicotine degradation pathways such as demethylation pathway in fun ...201324470788
potential for plant growth promotion by a consortium of stress-tolerant 2,4-dinitrotoluene-degrading bacteria: isolation and characterization of a military soil.the presence of explosives in soils and the interaction with drought stress and nutrient limitation are among the environmental factors that severely affect plant growth on military soils. in this study, we seek to isolate and identify the cultivable bacteria of a 2,4-dinitrotoluene (dnt) contaminated soil (ds) and an adjacent grassland soil (gs) of a military training area aiming to isolate new plant growth-promoting (pgp) and 2,4-dnt-degrading strains. metabolic profiling revealed disturbances ...201424467368
a multidisciplinary intervention to reduce infections of esbl- and ampc-producing, gram-negative bacteria at a university hospital.in response to a considerable increase in the infections caused by esbl/ampc-producing klebsiella pneumonia in 2008, a multidisciplinary intervention, with a main focus on antimicrobial stewardship, was carried out at one university hospital. four other hospitals were used as controls. stringent guidelines for antimicrobial treatment and prophylaxis were disseminated throughout the intervention hospital; cephalosporins were restricted for prophylaxis use only, fluoroquinolones for empiric use in ...201424466106
antibiotic resistance determinants in a pseudomonas putida strain isolated from a hospital.environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistance profiles of animal and human pathogens via horizontal gene transfer. pseudomonas putida strains are ubiquitous in soil and water but have been seldom isolated from humans. we have established a collection of p. putida strains isolated from in-patients in different hospitals in france. one of the isolated strains (hb3267) kills insects and is resistant to the majority of the an ...201424465371
pcrg protects the two long helical oligomerization domains of pcrv, by an interaction mediated by the intramolecular coiled-coil region of pcrg.pcrv is a hydrophilic translocator of type three secretion system (ttss) and a structural component of the functional translocon. c-terminal helix of pcrv is essential for its oligomerization at the needle tip. conformational changes within pcrv regulate the effector translocation. pcrg is a cytoplasmic regulator of ttss and forms a high affinity complex with pcrv. c-terminal residues of pcrg control the effector secretion.201424460624
3d structure modeling of alpha-amino acid ester hydrolase from xanthomonas rubrilineans.alpha-amino acid ester hydrolase (ec 3.1.1.43, aeh) is a promising biocatalyst for the production of semi-synthetic β-lactam antibiotics, penicillins and cephalosporins. the aeh gene from xanthomonas rubrilineans (xraeh) was recently cloned in this laboratory. the three-dimensional structure of xraeh was simulated using the homology modeling method for rational design experiments. the analysis of the active site was performed, and its structure was specified. the key amino acid residues in the a ...201324455184
the quest for a unified view of bacterial land colonization.exploring molecular mechanisms underlying bacterial water-to-land transition represents a critical start toward a better understanding of the functioning and stability of the terrestrial ecosystems. here, we perform comprehensive analyses based on a large variety of bacteria by integrating taxonomic, phylogenetic and metagenomic data, in the quest for a unified view that elucidates genomic, evolutionary and ecological dynamics of the marine progenitors in adapting to nonaquatic environments. we ...201424451209
electronic waste - an emerging threat to the environment of urban india.electronic waste or e-waste is one of the emerging problems in developed and developing countries worldwide. it comprises of a multitude of components with valuable materials, some containing toxic substances, that can have an adverse impact on human health and the environment. previous studies show that india has generated 0.4 million tons of e-waste in 2010 which may increase to 0.5 to 0.6 million tons by 2013-2014. coupled with lack of appropriate infrastructural facilities and procedures for ...201424444377
ex situ diet influences the bacterial community associated with the skin of red-eyed tree frogs (agalychnis callidryas).amphibians support symbiotic bacterial communities on their skin that protect against a range of infectious pathogens, including the amphibian chytrid fungus. the conditions under which amphibians are maintained in captivity (e.g. diet, substrate, enrichment) in ex situ conservation programmes may affect the composition of the bacterial community. in addition, ex situ amphibian populations may support different bacterial communities in comparison to in situ populations of the same species. this ...201424416427
public health implications of contamination of franc cfa (xaf) circulating in buea (cameroon) with drug resistant pathogens.studies in different parts of the world have implicated money as a vehicle for transmission of pathogens. such information which is necessary to facilitate infection control strategies is lacking in many sub-saharan countries including cameroon. this study analyzed the franc de la communauté financiere d'afrique (franc cfa), the currency used in cameroon and other countries in the central african sub-region, as a potential vehicle for transmission of pathogenic bacteria and fungi, particularly d ...201424401290
parallel histories of horizontal gene transfer facilitated extreme reduction of endosymbiont genomes in sap-feeding insects.bacteria confined to intracellular environments experience extensive genome reduction. in extreme cases, insect endosymbionts have evolved genomes that are so gene-poor that they blur the distinction between bacteria and endosymbiotically derived organelles such as mitochondria and plastids. to understand the host's role in this extreme gene loss, we analyzed gene content and expression in the nuclear genome of the psyllid pachypsylla venusta, a sap-feeding insect that harbors an ancient endosym ...201424398322
metabolic engineering of pseudomonas sp. strain vlb120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites.over the recent years the production of ehrlich pathway derived chemicals was shown in a variety of hosts such as escherichia coli, corynebacterium glutamicum, and yeast. exemplarily the production of isobutyric acid was demonstrated in escherichia coli with remarkable titers and yields. however, these examples suffer from byproduct formation due to the fermentative growth mode of the respective organism. we aim at establishing a new aerobic, chassis for the synthesis of isobutyric acid and othe ...201424397404
new and alternative approaches to tackling antibiotic resistance.multidrug-resistant bacteria are becoming more common and due to their multiplicity of mechanisms, they are frequently resistant to many if not all of the current antibiotics. this daunting spectre has been the target of many research efforts into conventional antibiotics and alternative approaches. this review focuses on the more recent advances in these fields with an overview on peptidomimetics, nanoparticles and their derivatives, fimh inhibitors, quorum sensing inhibition molecules, neoglyc ...201324381727
biosynthesis of rare hexoses using microorganisms and related enzymes.rare sugars, referred to as monosaccharides and their derivatives that rarely exist in nature, can be applied in many areas ranging from foodstuffs to pharmaceutical and nutrition industry, or as starting materials for various natural products and drug candidates. unfortunately, an important factor restricting the utilization of rare sugars is their limited availability, resulting from limited synthetic methods. nowadays, microbial and enzymatic transformations have become a very powerful tool i ...201324367410
cryotolerance strategies of pseudomonads isolated from the rhizosphere of himalayan plants.the cold stress biology of psychrotrophic pseudomonas strains isolated from the rhizosphere of himalayan plants have been explored to evaluate their cryotolerance characteristcs. pseudomonas strains were examined for stress metabolites, viz., exopolysaccharide (eps) production, intracellular sugar, polyols and amino acid content, ice nucleation activity, and their freezing survival at -10 and -40°c, respectively. high freezing survival was observed for the pseudomonas strains that were grown at ...201324363982
microbial community dynamics and effect of environmental microbial reservoirs on red-backed salamanders (plethodon cinereus).beneficial cutaneous bacteria on amphibians can protect against the lethal disease chytridiomycosis, which has devastated many amphibian species and is caused by the fungus batrachochytrium dendrobatidis. we describe the diversity of bacteria on red-backed salamanders (plethodon cinereus) in the wild and the stability of these communities through time in captivity using culture-independent illumina 16s rrna gene sequencing. after field sampling, salamanders were housed with soil from the field o ...201324335825
microbial community dynamics and effect of environmental microbial reservoirs on red-backed salamanders (plethodon cinereus).beneficial cutaneous bacteria on amphibians can protect against the lethal disease chytridiomycosis, which has devastated many amphibian species and is caused by the fungus batrachochytrium dendrobatidis. we describe the diversity of bacteria on red-backed salamanders (plethodon cinereus) in the wild and the stability of these communities through time in captivity using culture-independent illumina 16s rrna gene sequencing. after field sampling, salamanders were housed with soil from the field o ...201324335825
midgut microbial community of culex quinquefasciatus mosquito populations from india.the mosquito culex quinquefasciatus is a ubiquitous species that serves as a major vector for west nile virus and lymphatic filariasis. ingestion of bloodmeal by females triggers a series of physiological processes in the midgut and also exposes them to infection by these pathogens. the bacteria normally harbored in the midgut are known to influence physiology and can also alter the response to various pathogens. the midgut bacteria in female cx. quinquefasciatus mosquitoes collected over a larg ...201324312223
prevalence and antibiogram of extended spectrum β-lactamase (esbl) producing gram negative bacilli and further molecular characterization of esbl producing escherichia coli and klebsiella spp.resistance to a wide variety of common antimicrobials has made the proliferation of extended spectrum β-lactmase (esbl) producing strains a serious global health concern that has complicated treatment strategies. the high proportion of esbl producers among the enterobacteriaceae and the complex molecular epidemiology with diverse types of esbl genes are alarming. this study was undertaken to identify esbl production in various gram negative bacilli isolated and to further characterize esbl produ ...201324298468
commensal bacteria-dependent indole production enhances epithelial barrier function in the colon.microbiota have been shown to have a great influence on functions of intestinal epithelial cells (ecs). the role of indole as a quorum-sensing (qs) molecule mediating intercellular signals in bacteria has been well appreciated. however, it remains unknown whether indole has beneficial effects on maintaining intestinal barriers in vivo. in this study, we analyzed the effect of indole on ecs using a germ free (gf) mouse model. gf mice showed decreased expression of junctional complex molecules in ...201324278294
utilization of oleo-chemical industry by-products for biosurfactant production.biosurfactants are the surface active compounds produced by micro-organisms. the eco-friendly and biodegradable nature of biosurfactants makes their usage more advantageous over chemical surfactants. biosurfactants encompass the properties of dropping surface tension, stabilizing emulsions, promoting foaming and are usually non- toxic and biodegradable. biosurfactants offer advantages over their synthetic counterparts in many applications ranging from environmental, food, and biomedical, cosmeti ...201324262384
crystal structure of the γ-hydroxymuconic semialdehyde dehydrogenase from pseudomonas sp. strainwbc-3, a key enzyme involved in para-nitrophenol degradation.para-nitrophenol (pnp) is a highly toxic compound with threats to mammalian health. the pnpe-encoded γ-hydroxymuconic semialdehyde dehydrogenase catalyzes the reduction of γ-hydroxymuconic semialdehyde to maleylacetate in pseudomonas sp. strain wbc-3, playing a key role in the catabolism of pnp to krebs cycle intermediates. however, the catalyzing mechanism by pnpe has not been well understood.201324252642
biological activities of various extracts and chemical composition of trigonella monantha c. a. mey. subsp. monantha grown in iran.the objective of this research was to study the biological activities of various extracts and the chemical composition of the essential oil trigonella monantha c. a. mey. subsp. monantha by gas chromatography (gc) and gas chromatography-mass spectroscopy (gc-ms), from the compounds derived from the aerial parts. the overall results of t. monantha tests allow us to conclude various extracts [hexane extract (he), methanol extract (me) and chloroform extract (ce)] of the test total phenolic, abts a ...201224250546
loss of competition in the outside host environment generates outbreaks of environmental opportunist pathogens.environmentally transmitted pathogens face ecological interactions (e.g., competition, predation, parasitism) in the outside-host environment and host immune system during infection. despite the ubiquitousness of environmental opportunist pathogens, traditional epidemiology focuses on obligatory pathogens incapable of environmental growth. here we ask how competitive interactions in the outside-host environment affect the dynamics of an opportunist pathogen. we present a model coupling the class ...201324244752
antimicrobial activity of heterotrophic bacterial communities from the marine sponge erylus discophorus (astrophorida, geodiidae).heterotrophic bacteria associated with two specimens of the marine sponge erylus discophorus were screened for their capacity to produce bioactive compounds against a panel of human pathogens (staphylococcus aureus wild type and methicillin-resistant s. aureus (mrsa), bacillus subtilis, pseudomonas aeruginosa, acinetobacter baumanii, candida albicans and aspergillus fumigatus), fish pathogen (aliivibrio fischeri) and environmentally relevant bacteria (vibrio harveyi). the sponges were collected ...201324236081
structural and enzymatic characterization of the phosphotriesterase ophc2 from pseudomonas pseudoalcaligenes.organophosphates (ops) are neurotoxic compounds for which current methods of elimination are unsatisfactory; thus bio-remediation is considered as a promising alternative. here we provide the structural and enzymatic characterization of the recently identified enzyme isolated from pseudomonas pseudoalcaligenes dubbed ophc2. ophc2 belongs to the metallo-β-lactamase superfamily and exhibits an unusual thermal resistance and some op degrading abilities.201324223749
infectious scleritis: clinical spectrum and management outcomes in india.in this retrospective case series, we studied the predisposing factors, causative organisms, clinical spectrum, and outcomes of 12 cases of culture-proven infectious scleritis. nine of 12 patients had a history of preceding trauma (surgical or accidental). past surgical history included small-incision cataract surgery (4), pterygium surgery (1), and trabeculectomy (1). six patients had multifocal scleral abscesses due to pseudomonas, klebsiella, or nocardia. only 2 patients retained useful visio ...201324212312
a treatment plant receiving waste water from multiple bulk drug manufacturers is a reservoir for highly multi-drug resistant integron-bearing bacteria.the arenas and detailed mechanisms for transfer of antibiotic resistance genes between environmental bacteria and pathogens are largely unclear. selection pressures from antibiotics in situations where environmental bacteria and human pathogens meet are expected to increase the risks for such gene transfer events. we hypothesize that waste-water treatment plants (wwtps) serving antibiotic manufacturing industries may provide such spawning grounds, given the high bacterial densities present there ...201324204801
a microfluidic bioreactor with in situ sers imaging for the study of controlled flow patterns of biofilm precursor materials.a microfluidic bioreactor with an easy to fabricate nano-plasmonic surface is demonstrated for studies of biofilms and their precursor materials via surface enhanced raman spectroscopy (sers). the system uses a novel design to induce sheath flow confinement of a sodium citrate biofilm precursor stream against the sers imaging surface to measure spatial variations in the concentration profile. the unoptimised sers enhancement was approximately 2.5 × 10(4), thereby improving data acquisition time, ...201324172286
african mistletoes (loranthaceae); ethnopharmacology, chemistry and medicinal values: an update.mistletoes of the loranthaceae and viscaceae are hemiparasitic plants and their preparations in the form of injectable extracts, infusions, tinctures, fluid extracts or tea bags are widely used in various cultures in almost every continent to treat or manage various health problems including hypertension, diabetes mellitus, inflammatory conditions, irregular menstruations, menopause, epilepsy, arthritis, cancer, etc. the medicinal values of some species of mistletoes (loranthaceae) growing in th ...201324146518
genomic and functional analyses of the 2-aminophenol catabolic pathway and partial conversion of its substrate into picolinic acid in burkholderia xenovorans lb400.2-aminophenol (2-ap) is a toxic nitrogen-containing aromatic pollutant. burkholderia xenovorans lb400 possess an amn gene cluster that encodes the 2-ap catabolic pathway. in this report, the functionality of the 2-aminophenol pathway of b. xenovorans strain lb400 was analyzed. the amnrjbacdfehg cluster located at chromosome 1 encodes the enzymes for the degradation of 2-aminophenol. the absence of haba and habb genes in lb400 genome correlates with its no growth on nitrobenzene. rt-pcr analyses ...201324124510
bioaugmentation with petroleum-degrading consortia has a selective growth-promoting impact on crop plants germinated in diesel oil-contaminated soil.rhizoremediation is a complex type of green clean-up technology that involves both plants and the rhizosphere-associated microorganisms to decompose hazardous compounds. the success of the strategy strongly depends on plant tolerance towards the pollutant, as well as plant's interactions with the rhizospheric microbes. the microorganisms may be stimulated by the secreted root exudates, which results in an increased breakdown of contaminants in the rhizosphere. the main goal of this study was to ...201324078757
functional genes to assess nitrogen cycling and aromatic hydrocarbon degradation: primers and processing matter.targeting sequencing to genes involved in key environmental processes, i.e., ecofunctional genes, provides an opportunity to sample nature's gene guilds to greater depth and help link community structure to process-level outcomes. vastly different approaches have been implemented for sequence processing and, ultimately, for taxonomic placement of these gene reads. the overall quality of next generation sequence analysis of functional genes is dependent on multiple steps and assumptions of unknow ...201324062736
light based anti-infectives: ultraviolet c irradiation, photodynamic therapy, blue light, and beyond.owing to the worldwide increase in antibiotic resistance, researchers are investigating alternative anti-infective strategies to which it is supposed microorganisms will be unable to develop resistance. prominent among these strategies, is a group of approaches which rely on light to deliver the killing blow. as is well known, ultraviolet light, particularly uvc (200-280 nm), is germicidal, but it has not been much developed as an anti-infective approach until recently, when it was realized that ...201324060701
evaluation and biochemical characterization of a distinctive pyoverdin from a pseudomonas isolated from chickpea rhizosphere.microbial siderophores confiscate the available ferric ions around the roots and trigger a reaction resulting in plant growth promotion. in our study, a high level of siderophore production was observed from a newly isolated pseudomonas sp. from the rhizosphere of chickpea plants. under an iron depleted condition in standard succinic acid medium a 1000 μgml(-1) of siderophore production was achieved. increasing the concentration of iron showed an inverse relationship between growth and sideropho ...201224031875
optimization of extracellular thermophilic highly alkaline lipase from thermophilic bacillus sp isolated from hotspring of arunachal pradesh, india.studies on lipase production were carried out with a bacterial strain (bacillus sp lbn 2) isolated from soil sample of hotspring of arunachal pradesh, india. the cells were cultivated in a mineral medium with maximum production at 1% groundnut oil. the optimum temperature and initial medium ph for lipase production by the organism were 50(0)c and 9.0 respectively. the molecular mass was found to be 33kda by sds page. the optimal ph and temperature for activity were 10 and 60(0)c respectively. th ...201224031801
hydrolytic potential of a psychrotrophic pseudomonas isolated from refrigerated raw milk.the production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a pseudomonas sp. belonging to the pseudomonas jenssenii group, was studied. this bacterium produced proteolytic and lipolytic enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). high levels of α-glucosidase were produced in skim milk broth. hydrolytic enzymes detected in skim milk broth are of particular concern, indicating ...201124031781
prevalence, aetiology and antibiotic resistance profiles of coagulase negative staphylococci isolated in a teaching hospital.in this paper we carried out a study about prevalence of the clinically significant coagulase negative staphylococcal (cns) isolates found in an university hospital. two hundred four cns isolates from 191 patients obtained between the period of 1998 to 2002, were studied. about 27% (52/191) of the infection cases studied were confirmed as cns-associated diseases. blood stream infection (bsi) was the most frequent cns associated-disease (25%; 13/52). the great majority of the bsi was verified in ...201124031628
degradation of h-acid by free and immobilized cells of alcaligenes latus.alcaligenes latus, isolated from industrial effluent, was able to grow in mineral salts medium with 50 ppm (0.15 mm) of h-acid as a sole source of carbon. immobilization of alcaligenes latus in ca-alginate and polyurethane foam resulted in cells embedded in the matrices. when free cells and immobilized cells were used for biodegradation studies at concentration ranging from 100 ppm (0.3 mm) to 500 ppm (1.15 mm) degradation rate was enhanced with immobilized cells. cells immobilized in polyuretha ...201024031573
isolation and identification of profenofos degrading bacteria.an enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from hubei province of central china. two pure bacterial cultures, named w and y, were isolated and subsequently characterized by sequencing of 16s rrna genes and biochemical tests. isolate w showed 96% similarity to the 16s rrna gene of a pseudomonas putida unlike y which showed 99% similarity to the 16s rrna gene of burkholderia gladioli. both strains grew well at ph ...200924031438
aerobic bacterial microflora of broad-snouted caiman (caiman latirostris) oral cavity and cloaca, originating from parque zoológico arruda câmara, paraíba, brazil.the objective of this study was to isolate and identify the aerobic bacterial microflora from the oral cavity mucosa and cloaca's samples, collected from broad-snouted caiman (caiman latirostris), born and bred in captivity at parque zoológico arruda câmara, joão pessoa, paraíba, brazil. the most common bacteria were staphylococcus sp. (14.74%), corynebacterium sp. (13.68%), escherichia coli (13.68%) and shigella sp.(11.58%), and the less common were citrobacter sp. (1.05%), klebsiella pneumonia ...200924031343
isolation and preliminary characterization of a o-nitrobenzaldehyde-degrading alcaligenes sp. nd1.this paper reports the isolation and characterization of a new o-nitrobenzaldehyde (onba)-degrading bacterium, alcaligenes sp. nd1. nd1 degraded almost all onba (100 mg l(-1)) in m9 medium within 36 hours. the key enzyme(s) involved in the initial biodegradation was a constitutively intracellular enzyme(s). this bacterium has great potential utility for bioremediation.200824031306
characterization of epiphytic bacterial communities from grapes, leaves, bark and soil of grapevine plants grown, and their relations.despite its importance in plant health and crop quality, the diversity of epiphytic bacteria on grape berries and other plant parts, like leaves and bark, remains poorly described, as does the role of telluric bacteria in plant colonization. in this study, we compare the bacterial community size and structure in vineyard soils, as well as on grapevine bark, leaves and berries. analyses of culturable bacteria revealed differences in the size and structure of the populations in each ecosystem. the ...201324023666
permanent draft genome sequence of comamonas testosteroni kf-1.comamonas testosteroni kf-1 is a model organism for the elucidation of the novel biochemical degradation pathways for xenobiotic 4-sulfophenylcarboxylates (spc) formed during biodegradation of synthetic 4-sulfophenylalkane surfactants (linear alkylbenzenesulfonates, las) by bacterial communities. here we describe the features of this organism, together with the complete genome sequence and annotation. the 6,026,527 bp long chromosome (one sequencing gap) exhibits an average g+c content of 61.79% ...201323991256
cloning and characterization of ef-tu and ef-ts from pseudomonas aeruginosa.we have cloned genes encoding elongation factors ef-tu and ef-ts from pseudomonas aeruginosa and expressed and purified the proteins to greater than 95% homogeneity. sequence analysis indicated that p. aeruginosa ef-tu and ef-ts are 84% and 55% identical to e. coli counterparts, respectively. p. aeruginosa ef-tu was active when assayed in gdp exchange assays. kinetic parameters for the interaction of ef-tu with gdp in the absence of ef-ts were observed to be k m = 33 μm, k cat (obs) = 0.003 s(-1 ...201323984384
bacteriuria amongst pregnant women in the buea health district, cameroon: prevalence, predictors, antibiotic susceptibility patterns and diagnosis.bacteriuria is associated with significant maternal and foetal risks. however, its prevalence is not known in our community.201323976983
microarray-based identification of differentially expressed genes in intracellular brucella abortus within raw264.7 cells.brucella spp. is a species of facultative intracellular gram-negative bacteria that induces abortion and causes sterility in domesticated mammals and chronic undulant fever in humans. important determinants of brucella's virulence and potential for chronic infection include the ability to circumvent the host cell's internal surveillance system and the capability to proliferate within dedicated and non-dedicated phagocytes. hence, identifying genes necessary for intracellular survival may hold th ...201323950864
prevention and treatment of biofilms by hybrid- and nanotechnologies.bacteria growing as adherent biofilms are difficult to treat and frequently develop resistance to antimicrobial agents. to counter biofilms, various approaches, including prevention of bacterial surface adherence, application of device applicators, and assimilation of antimicrobials in targeted drug delivery machinery, have been utilized. these methods are also combined to achieve synergistic bacterial killing. this review discusses various multimodal technologies, presents general concepts, and ...201323946652
promise for plant pest control: root-associated pseudomonads with insecticidal activities.insects are an important and probably the most challenging pest to control in agriculture, in particular when they feed on belowground parts of plants. the application of synthetic pesticides is problematic owing to side effects on the environment, concerns for public health and the rapid development of resistance. entomopathogenic bacteria, notably bacillus thuringiensis and photorhabdus/xenorhabdus species, are promising alternatives to chemical insecticides, for they are able to efficiently k ...201323914197
culture-independent investigation of the microbiome associated with the nematode acrobeloides maximus.symbioses between metazoans and microbes are widespread and vital to many ecosystems. recent work with several nematode species has suggested that strong associations with microbial symbionts may also be common among members of this phylu. in this work we explore possible symbiosis between bacteria and the free living soil bacteriovorous nematode acrobeloides maximus.201323894287
diversity and phylogenetic analyses of bacteria from a shallow-water hydrothermal vent in milos island (greece).studies of shallow-water hydrothermal vents have been lagging behind their deep-sea counterparts. hence, the importance of these systems and their contribution to the local and regional diversity and biogeochemistry is unclear. this study analyzes the bacterial community along a transect at the shallow-water hydrothermal vent system of milos island, greece. the abundance and biomass of the prokaryotic community is comparable to areas not affected by hydrothermal activity and was, on average, 1.3 ...201323847607
structural and kinetic characterization of two 4-oxalocrotonate tautomerases in methylibium petroleiphilum strain pm1.methylibium petroleiphilum strain pm1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. it has two operons, dmpi and dmpii, that code for the enzymes in a pair of parallel meta-fission pathways. in order to understand the roles of the pathways, the 4-oxalocrotonate tautomerase (4-ot) isozyme from each pathway was characterized. tautomerase i and tautomerase ii have the lowest pairwis ...201323831510
screening of sds-degrading bacteria from car wash wastewater and study of the alkylsulfatase enzyme activity.sodium dodecyl sulfate (sds) is one of the main surfactant components in detergents and cosmetics, used in high amounts as a detergent in products such as shampoos, car wash soap and toothpaste. therefore, its bioremediation by suitable microorganisms is important. alkylsulfatase is an enzyme that hydrolyses sulfate -ester bonds to give inorganic sulfate and alcohol. the purpose of this study was to isolate sds-degrading bacteria from tehran city car wash wastewater, study bacterial alkylsulfata ...201323825734
genome analysis and physiological comparison of alicycliphilus denitrificans strains bc and k601(t.).the genomes of the betaproteobacteria alicycliphilus denitrificans strains bc and k601(t) have been sequenced to get insight into the physiology of the two strains. strain bc degrades benzene with chlorate as electron acceptor. the cyclohexanol-degrading denitrifying strain k601(t) is not able to use chlorate as electron acceptor, while strain bc cannot degrade cyclohexanol. the 16s rrna sequences of strains bc and k601(t) are identical and the fatty acid methyl ester patterns of the strains are ...201323825601
responses of soil bacterial and fungal communities to extreme desiccation and rewetting.the microbial response to summer desiccation reflects adaptation strategies, setting the stage for a large rainfall-induced soil co2 pulse upon rewetting, an important component of the ecosystem carbon budget. in three california annual grasslands, the present (dna-based) and potentially active (rna-based) soil bacterial and fungal communities were tracked over a summer season and in response to controlled rewetting of intact soil cores. phylogenetic marker genes for bacterial (16s) and fungal ( ...201323823489
postoperative differences between colonization and infection after pediatric cardiac surgery-a propensity matched analysis.the objective of this study was to identify the postoperative risk factors associated with the conversion of colonization to postoperative infection in pediatric patients undergoing cardiac surgery.201323819455
evolution of rep diversity: a comparative study.repetitive extragenic palindromic elements (reps) constitute a group of bacterial genomic repeats known for their high abundance and several roles in host cells´ physiology. we analyzed the phylogenetic distribution of particular rep classes in genomic sequences of sixty-three bacterial strains belonging to the pseudomonas fluorescens species complex and ten strains of stenotrophomonas sp., in order to assess intraspecific rep diversity and to gain insight into long-term rep evolution.201323758774
purification and identification of trichloroethylene induced proteins from stenotrophomonas maltophilia pm102 by immuno-affinity-chromatography and maldi-tof mass spectrometry.a novel bacterial isolate capable of growth on trichloroethylene as the sole carbon source was identified as stenotrophomonas maltophilia pm102 by 16s rdna sequencing (genbank acc.no. jq797560). serum was obtained from a rabbit immunized with the total protein extracted from the pm102 isolate grown in 0.2% tce with 0.2% peptone. antibodies to the common antigens were removed by preadsorbing the serum antibody on total protein extracted from the pm102 strain grown in 0.2% peptone. western blot wi ...201323741645
characterisation of phosphate solubilising bacteria in sandy loam soil under chickpea cropping system.with the aim to explore the possible role of phosphate-solubilizing bacteria (psb) in phosphorus (p) cycling in agricultural soils, we isolated psb inhabiting naturally in the sandy loam soils under chickpea cropping of patiala (punjab state). a total of 31 bacterial isolates showing solubilizing activities were isolated on pikovskaya agar plates. the potent phosphate solubilizers were selected for further characterization. these isolates were shown to belong to the genera pseudomonas and serrat ...201123729877
characterisation of phosphate solubilising bacteria in sandy loam soil under chickpea cropping system.with the aim to explore the possible role of phosphate-solubilizing bacteria (psb) in phosphorus (p) cycling in agricultural soils, we isolated psb inhabiting naturally in the sandy loam soils under chickpea cropping of patiala (punjab state). a total of 31 bacterial isolates showing solubilizing activities were isolated on pikovskaya agar plates. the potent phosphate solubilizers were selected for further characterization. these isolates were shown to belong to the genera pseudomonas and serrat ...201123729877
the catalytic roles of p185 and t188 and substrate-binding loop flexibility in 3α-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni.3α-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni reversibly catalyzes the oxidation of androsterone with nad(+) to form androstanedione and nadh. structurally the substrate-binding loop of the residues, t188-k208, is unresolved, while binding with nad(+) causes the appearance of t188-p191 in the binary complex. this study determines the functional roles of the flexible substrate-binding loop in conformational changes and enzyme catalysis. a stopped-flow study reveal ...201323717450
fluopsin c induces oncosis of human breast adenocarcinoma cells.fluopsin c, an antibiotic isolated from pseudomonas jinanesis, has shown antitumor effects on several cancer cell lines. in the current study, the oncotic cell death induced by fluopsin c was investigated in human breast adenocarcinoma cells in vitro.201323708552
antiviral activity of natural products extracted from marine organisms.many epidemics have broken out over the centuries. hundreds and thousands of humans have died over a disease. available treatments for infectious diseases have always been limited. some infections are more deadly than the others, especially viral pathogens. these pathogens have continuously resisted all kinds of medical treatment, due to a need for new treatments to be developed. drugs are present in nature and are also synthesized in vitro and they help in combating diseases and restoring healt ...201123678429
the ethnobotanical, phytochemical and mineral analyses of phragmanthera incana (klotzsch), a species of mistletoe growing on three plant hosts in south-western nigeria.mistletoe is collected wildly on various plants and phragmanthera incana is noted to grow on different plant hosts. this study was designed to carry out the ethnobotanical survey, phytochemical and mineral analyses of phragmanthera incana, a species of mistletoe growing on three plant hosts namely cocoa (theobroma cacao), kolanut (cola nitida) and bush mango (irvingia gabonensis). mistletoe samples were identified at the forestry research institute of nigeria herbarium. phragmanthera incana was ...201323675287
halomonas sp. bs4, a biosurfactant producing halophilic bacterium isolated from solar salt works in india and their biomedical importance.halophilic bacteria were isolated from thamaraikulam solar salt works in india. after routine biosurfactant screening by various methods, the biosurfactant producing bacteria, halomonas sp bs4 was confirmed by 16 s rrna sequencing. the growth optimization of halomonas sp bs4 revealed their optimum growth at 8% nacl and 6-8 ph in the growth medium. further the partially purified biosurfactants were characterized by tlc, ftir and gc-ms analysis. gc-ms results revealed that, the partial purified bi ...201323667807
secondary bacterial infections of buruli ulcer lesions before and after chemotherapy with streptomycin and rifampicin.buruli ulcer (bu), caused by mycobacterium ulcerans is a chronic necrotizing skin disease. it usually starts with a subcutaneous nodule or plaque containing large clusters of extracellular acid-fast bacilli. surrounding tissue is destroyed by the cytotoxic macrolide toxin mycolactone produced by microcolonies of m. ulcerans. skin covering the destroyed subcutaneous fat and soft tissue may eventually break down leading to the formation of large ulcers that progress, if untreated, over months and ...201323658847
pivotal role of p450-p450 interactions in cyp3a4 allostery: the case of α-naphthoflavone.we investigated the relationship between oligomerization of cyp3a4 (cytochrome p450 3a4) and its response to anf (α-naphthoflavone), a prototypical heterotropic activator. the addition of anf resulted in over a 2-fold increase in the rate of cyp3a4-dependent debenzylation of 7-bfc [7-benzyloxy-4-(trifluoromethyl)coumarin] in hlm (human liver microsomes), but failed to produce activation in bd supersomes or baculosomes containing recombinant cyp3a4 and nadph-cpr (cytochrome p450 reductase). howev ...201323651100
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