Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| molecular cloning and functional expression of a δ9- fatty acid desaturase from an antarctic pseudomonas sp. a3. | fatty acid desaturase enzymes play an essential role in the synthesis of unsaturated fatty acids. pseudomonas sp. a3 was found to produce a large amount of palmitoleic and oleic acids after incubation at low temperatures. using polymerase chain reaction (pcr), a novel δ9- fatty acid desaturase gene was isolated, cloned, and successfully expressed in escherichia coli. the gene was designated as pa3fad9 and has an open reading frame of 1,185 bp which codes for 394 amino acids with a predicted mole ... | 2016 | 27494717 |
| cellshape: a user-friendly image analysis tool for quantitative visualization of bacterial cell factories inside. | visualization of the intracellular constituents of individual bacteria while performing as live biocatalysts is in principle doable through more or less sophisticated fluorescence microscopy. unfortunately, rigorous quantitation of the wealth of data embodied in the resulting images requires bioinformatic tools that are not widely extended within the community-let alone that they are often subject to licensing that impedes software reuse. in this context we have developed cellshape, a user-frien ... | 2017 | 27492366 |
| insights into the genome sequences of an n-acyl homoserine lactone molecule producing two pseudomonas spp. isolated from the arctic. | we report for the first time the draft genome sequence of two psychrotrophic pseudomonas species, pseudomonas simiae rgcb 73 and pseudomonas brenneri rgcb 108, from the arctic that produce more than one acyl homoserine lactone molecule of varied n-acyl length. the study confirms the presence of a luxr-luxi (type) mediated quorum-sensing system in both the pseudomonas species and enables us to understand the role of quorum sensing in their survival in extremely cold environments. | 2016 | 27491995 |
| genetic dissection of the regulatory network associated with high c-di-gmp levels in pseudomonas putida kt2440. | most bacteria grow in nature forming multicellular structures named biofilms. the bacterial second messenger cyclic diguanosine monophosphate (c-di-gmp) is a key player in the regulation of the transition from planktonic to sessile lifestyles and this regulation is crucial in the development of biofilms. in pseudomonas putida kt2440, rup4959, a multidomain response regulator with diguanylate cyclase activity, when overexpressed causes an increment in the intracellular levels of c-di-gmp that giv ... | 2016 | 27489550 |
| mapping the patent landscape of synthetic biology for fine chemical production pathways. | a goal of synthetic biology bio-foundries is to innovate through an iterative design/build/test/learn pipeline. in assessing the value of new chemical production routes, the intellectual property (ip) novelty of the pathway is important. exploratory studies can be carried using knowledge of the patent/ip landscape for synthetic biology and metabolic engineering. in this paper, we perform an assessment of pathways as potential targets for chemical production across the full catalogue of reachable ... | 2016 | 27489206 |
| expression of a grapevine nac transcription factor gene is induced in response to powdery mildew colonization in salicylic acid-independent manner. | tissue colonization by grape powdery mildew (pm) pathogen erysiphe necator (schw.) burr triggers a major remodeling of the transcriptome in the susceptible grapevine vitis vinifera l. while changes in the expression of many genes bear the signature of salicylic acid (sa) mediated regulation, the breadth of pm-induced changes suggests the involvement of additional regulatory networks. to explore pm-associated gene regulation mediated by other sa-independent systems, we designed a microarray exper ... | 2016 | 27488171 |
| lactobacillus oligofermentans glucose, ribose and xylose transcriptomes show higher similarity between glucose and xylose catabolism-induced responses in the early exponential growth phase. | lactobacillus oligofermentans has been mostly isolated from cold-stored packaged meat products in connection with their spoilage, but its precise role in meat spoilage is unknown. it belongs to the l. vaccinostercus group of obligate heterofermentative lactobacilli that generally ferment pentoses (e.g. xylose and ribose) more efficiently than hexoses (e.g. glucose). however, more efficient hexose utilization can be induced. the regulation mechanisms of the carbohydrate catabolism in such bacteri ... | 2016 | 27487841 |
| rhizosphere selection of pseudomonas putida kt2440 variants with increased fitness associated to changes in gene expression. | as the interface between plant roots and soil, the rhizosphere is a complex environment where nutrients released by the plant promote microbial growth. increasing evidences indicate that the plant also exerts a selective pressure on microbial populations in the rhizosphere, favouring colonization by certain groups. in this work, we have designed an experimental setup to begin analysing the evolution of a specific bacterial population in the rhizosphere, using pseudomonas putida kt2440 as model o ... | 2016 | 27487165 |
| three coa transferases involved in the production of short chain fatty acids in porphyromonas gingivalis. | butyryl-coa:acetate coa transferase, which produces butyrate and acetyl-coa from butyryl-coa and acetate, is responsible for the final step of butyrate production in bacteria. this study demonstrates that in the periodontopathogenic bacterium porphyromonas gingivalis this reaction is not catalyzed by pgn_1171, previously annotated as butyryl-coa:acetate coa transferase, but by three distinct coa transferases, pgn_0725, pgn_1341, and pgn_1888. gas chromatography/mass spectrometry (gc-ms) and spec ... | 2016 | 27486457 |
| thusin, a novel two-component lantibiotic with potent antimicrobial activity against several gram-positive pathogens. | due to the rapidly increasing prevalence of multidrug-resistant bacterial strains, the need for new antimicrobial drugs to treat infections has become urgent. bacteriocins, which are antimicrobial peptides of bacterial origin, are considered potential alternatives to conventional antibiotics and have attracted widespread attention in recent years. among these bacteriocins, lantibiotics, especially two-component lantibiotics, exhibit potent antimicrobial activity against some clinically relevant ... | 2016 | 27486447 |
| analysis of plant growth-promoting effects of fluorescent pseudomonas strains isolated from mentha piperita rhizosphere and effects of their volatile organic compounds on essential oil composition. | many species or strains of the genus pseudomonas have been characterized as plant growth promoting rhizobacteria (pgpr). we used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent pseudomonas strains in the rhizosphere of commercially grown mentha piperita (peppermint). biochemical techniques, amplified rdna restriction analysis (ardra), and 16s rrna gene sequence analysis revealed that the majority of the isolated native fluorescent strains were p. puti ... | 2016 | 27486441 |
| a luxr homolog in a cottonwood tree endophyte that activates gene expression in response to a plant signal or specific peptides. | homologs of the luxr acyl-homoserine lactone (ahl) quorum-sensing signal receptor are prevalent in proteobacteria isolated from roots of the eastern cottonwood tree, populus deltoides many of these isolates possess an orphan luxr homolog, closely related to oryr from the rice pathogen xanthomonas oryzae oryr does not respond to ahl signals but, instead, responds to an unknown plant compound. we discovered an oryr homolog, pipr, in the cottonwood endophyte pseudomonas sp. strain gm79. the genes a ... | 2016 | 27486195 |
| effect of shear stress on pseudomonas aeruginosa isolated from the cystic fibrosis lung. | chronic colonization of the lungs by pseudomonas aeruginosa is one of the major causes of morbidity and mortality in cystic fibrosis (cf) patients. to gain insights into the characteristic biofilm phenotype of p. aeruginosa in the cf lungs, mimicking the cf lung environment is critical. we previously showed that growth of the non-cf-adapted p. aeruginosa pao1 strain in a rotating wall vessel, a device that simulates the low fluid shear (ls) conditions present in the cf lung, leads to the formati ... | 2016 | 27486191 |
| biosorption of aluminum through the use of non-viable biomass of pseudomonas putida. | living and non-living biomass of pseudomonas putida a (atcc 12633) was used as biosorbent for the removing of al(3+) from aqueous solutions. the process was stable with time, efficient at ph 4.3 and between 15°c and 42°c. two isotherms models were applied to describe the interaction between the biosorbent and al(3+). non-living biomass of p. putida a (atcc 12633) was found to be the most efficient at adsorbing al(3+) with a maximum sorption capacity of 0.55mg al(3+)/gr adsorbent and with 36×10(5 ... | 2016 | 27485814 |
| interactive effects of phosphorus and pseudomonas putida on chickpea (cicer arietinum l.) growth, nutrient uptake, antioxidant enzymes and organic acids exudation. | phosphorus (p) availability in alkaline soils of arid and semi-arid regions is a major constraint for decreased crop productivity. use of plant growth promoting rhizobacteria (pgpr) may enhance plant growth through the increased plant antioxidation activity. additionally, pgpr may increase nutrient uptake by plants as a result of induced root exudation and rhizosphere acidification. the current study was aimed to investigate combined effects of p and pesudomonas putida (pgpr) on chickpea growth ... | 2016 | 27485620 |
| flra, flrb and flrc regulate adhesion by controlling the expression of critical virulence genes in vibrio alginolyticus. | adhesion is an important virulence trait of vibrio alginolyticus. bacterial adhesion is influenced by environmental conditions; however, the molecular mechanism underlying this effect remains unknown. the expression levels of flra, flrb and flrc were significantly downregulated in adhesion-deficient v. alginolyticus strains cultured under cu(2+), pb(2+), hg(2+) and low-ph stresses. silencing these genes led to deficiencies in adhesion, motility, flagellar assembly, biofilm formation and exopolys ... | 2016 | 27485498 |
| new family of biosensors for monitoring btx in aquatic and edaphic environments. | benzene, toluene, ethylbenzene and xylenes (btex) contamination is a serious threat to public health and the environment, and therefore, there is an urgent need to detect its presence in nature. the use of whole-cell reporters is an efficient, easy-to-use and low-cost approach to detect and follow contaminants outside specialized laboratories; this is especially important in oil spills that are frequent in marine environments. the aim of this study is the construction of a bioreporter system and ... | 2016 | 27484951 |
| risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry. | the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ... | 2017 | 27478029 |
| two distinct cardiolipin synthases operate in agrobacterium tumefaciens. | cardiolipin (cl) is a universal component of energy generating membranes. in most bacteria, it is synthesized via the condensation of two molecules phosphatidylglycerol (pg) by phospholipase d-type cardiolipin synthases (pld-type cls). in the plant pathogen and natural genetic engineer agrobacterium tumefaciens cl comprises up to 15% of all phospholipids in late stationary growth phase. a. tumefaciens harbors two genes, atu1630 (cls1) and atu2486 (cls2), coding for pld-type cls. heterologous exp ... | 2016 | 27472399 |
| structural and catalytic characterization of a fungal baeyer-villiger monooxygenase. | baeyer-villiger monooxygenases (bvmos) are biocatalysts that convert ketones to esters. due to their high regio-, stereo- and enantioselectivity and ability to catalyse these reactions under mild conditions, they have gained interest as alternatives to chemical baeyer-villiger catalysts. despite their widespread occurrence within the fungal kingdom, most of the currently characterized bvmos are from bacterial origin. here we report the catalytic and structural characterization of bvmoafl838 from ... | 2016 | 27472055 |
| structural analysis and biological toxicity of aflatoxins b1 and b2 degradation products following detoxification by ocimum basilicum and cassia fistula aqueous extracts. | this study showed the comparison between ocimum basilicum and cassia fistula (leaves and branch) aqueous extracts for their ability to detoxify of aflatoxins b1 and b2 (afb1; 100 μg l(-1) and afb2; 50 μg l(-1)) by in vitro assays and decontamination studies. results indicated that o. basilicum leaves extract was found to be highly significant (p < 0.05) in degrading afb1 and afb2, i.e., 90.4 and 88.6%, respectively. however, o. basilicum branch, c. fistula leaves and branch extracts proved to be ... | 2016 | 27471501 |
| development of a quadruplex loop-mediated isothermal amplification assay for field detection of four vibrio species associated with fish disease. | a quadruplex loop-mediated isothermal amplification (lamp) method was developed to detect four vibrio species, including vibrio ichthyoenteri, vibrio parahaemolyticus, vibrio scophthalmi, and vibrio vulnificus, simultaneously. four sets of species-specific primers were designed with different restriction sites contained in the inner primers. the quadruplex lamp method could distinguish four vibrio species via the subsequent restriction enzyme analysis. the sensitivity of the quadruplex lamp meth ... | 2016 | 27468405 |
| engineering salmonella as intracellular factory for effective killing of tumour cells. | salmonella have many desirable properties as antitumour-agent due to its ability to proliferate inside tumours and induce tumour regression. additionally, this bacterium can be genetically engineered to deliver therapeutic proteins intratumourally. the main limitation of this approach is the efficient release of therapeutic molecules from intratumoural bacteria. here we have developed an inducible autolysis system based in the lysis operon of the lambda phage that, in response to anhydrotetracyc ... | 2016 | 27464652 |
| sublethal concentrations of antibiotics cause shift to anaerobic metabolism in listeria monocytogenes and induce phenotypes linked to antibiotic tolerance. | the human pathogenic bacterium listeria monocytogenes is exposed to antibiotics both during clinical treatment and in its saprophytic lifestyle. as one of the keys to successful treatment is continued antibiotic sensitivity, the purpose of this study was to determine if exposure to sublethal antibiotic concentrations would affect the bacterial physiology and induce antibiotic tolerance. transcriptomic analyses demonstrated that each of the four antibiotics tested caused an antibiotic-specific ge ... | 2016 | 27462313 |
| antimicrobial activities of bacteria associated with the brown alga padina pavonica. | macroalgae belonging to the genus padina are known to produce antibacterial compounds that may inhibit growth of human- and animal pathogens. hitherto, it was unclear whether this antibacterial activity is produced by the macroalga itself or by secondary metabolite producing epiphytic bacteria. here we report antibacterial activities of epiphytic bacteria isolated from padina pavonica (peacocks tail) located on northern coast of tunisia. eighteen isolates were obtained in pure culture and tested ... | 2016 | 27462308 |
| pcr-independent detection of bacterial species-specific 16s rrna at 10 fm by a pore-blockage sensor. | a pcr-free, optics-free device is used for the detection of escherichia coli (e. coli) 16s rrna at 10 fm, which corresponds to ~100-1000 colony forming units/ml (cfu/ml) depending on cellular rrna levels. the development of a rapid, sensitive, and cost-effective nucleic acid detection platform is sought for the detection of pathogenic microbes in food, water and body fluids. since 16s rrna sequences are species specific and are present at high copy number in viable cells, these nucleic acids off ... | 2016 | 27455337 |
| proteome analysis of pseudomonas putida f1 genes induced in soil environments. | knowledge of the gene expression dynamics of a single soil bacterial strain contributes to the understanding of its behaviour, physiological state and surrounding microenvironment. genes expressed in soil environments rather than in laboratory media are considered to particularly relevant. here, we compared genome-wide gene expression profiles of the bacterium pseudomonas putida f1 inoculated in three different types of nonsterile soils deduced using proteome analysis via sodium dodecyl sulfate- ... | 2016 | 27452675 |
| a novel papillation assay for the identification of genes affecting mutation rate in pseudomonas putida and other pseudomonads. | formation of microcolonies (papillae) permits easy visual screening of mutational events occurring in single colonies of bacteria. in this study, we have established a novel papillation assay employable in a wide range of pseudomonads including pseudomonas aeruginosa and pseudomonas putida for monitoring mutation frequency in distinct colonies. with the aid of this assay, we conducted a genome-wide search for the factors affecting mutation frequency in p. putida. screening ∼27,000 transposon mut ... | 2016 | 27447898 |
| carbon sources for polyhydroxyalkanoates and an integrated biorefinery. | polyhydroxyalkanoates (phas) are a group of bioplastics that have a wide range of applications. extensive progress has been made in our understanding of phas' biosynthesis, and currently, it is possible to engineer bacterial strains to produce phas with desired properties. the substrates for the fermentative production of phas are primarily derived from food-based carbon sources, raising concerns over the sustainability of their production in terms of their impact on food prices. this paper give ... | 2016 | 27447619 |
| "omics" tools for better understanding the plant-endophyte interactions. | endophytes, which mostly include bacteria, fungi and actinomycetes, are the endosymbionts that reside asymptomatically in plants for at least a part of their life cycle. they have emerged as a valuable source of novel metabolites, industrially important enzymes and as stress relievers of host plant, but still many aspects of endophytic biology are unknown. functions of individual endophytes are the result of their continuous and complex interactions with the host plant as well as other members o ... | 2016 | 27446181 |
| plants rather than mineral fertilization shape microbial community structure and functional potential in legacy contaminated soil. | plant-microbe interactions are of particular importance in polluted soils. this study sought to determine how selected plants (horseradish, black nightshade and tobacco) and npk mineral fertilization shape the structure of soil microbial communities in legacy contaminated soil and the resultant impact of treatment on the soil microbial community functional potential. to explore these objectives, we combined shotgun metagenomics and 16s rrna gene amplicon high throughput sequencing with data anal ... | 2016 | 27446035 |
| genomic potential of stenotrophomonas maltophilia in bioremediation with an assessment of its multifaceted role in our environment. | the gram negative bacterium stenotrophomonas is rapidly evolving as a nosocomial pathogen in immuno-compromised patients. treatment of stenotrophomonas maltophilia infections is problematic because of their increasing resistance to multiple antibiotics. this article aims to review the multi-disciplinary role of stenotrophomonas in our environment with special focus on their metabolic and genetic potential in relation to bioremediation and phytoremediation. current and emerging treatments and dia ... | 2016 | 27446008 |
| production of glucaric acid from myo-inositol in engineered pichia pastoris. | a potential myo-inositol oxygenase (ppmiox) was identified as a functional enzyme and a glucaric acid synthetic pathway was firstly constructed in pichia pastoris. coexpression of the native ppmiox and the urinate dehydrogenase (udh) from pseudomonas putida kt2440 led to obvious accumulation of glucaric acid (90.46±0.04mg/l) from myo-inositol whereas no glucaric acid was detected from glucose. in comparison, coexpression of the heterologous mouse miox (mmiox) and udh resulted in higher titers of ... | 2016 | 27444324 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) can precisely discriminate the lineages of listeria monocytogenes and species of listeria. | the genetic lineages of listeria monocytogenes and other species of the genus listeria are correlated with pathogenesis in humans. although matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has become a prevailing tool for rapid and reliable microbial identification, the precise discrimination of listeria species and lineages remains a crucial issue in clinical settings and for food safety. in this study, we constructed an accurate and reliable ms databa ... | 2016 | 27442502 |
| content of cardiolipin of the membrane and sensitivity to cationic surfactants in pseudomonas putida. | to establish the role of cardiolipin (cl) of the membrane in response to the presence of tetradecyltrimethylammonium in pseudomonas putida a (atcc 12633). | 2016 | 27442261 |
| physiological and transcriptomic analyses reveal mechanistic insight into the adaption of marine bacillus subtilis c01 to alumina nanoparticles. | an increasing number of studies have investigated the effects of nanoparticles (nps) on microbial systems; however, few existing reports have focused on the defense mechanisms of bacteria against nps. whether secondary metabolism biosynthesis is a response to np stress and contributes to the adaption of bacteria to nps is unclear. here, a significant induction in the surfactin production and biofilm formation were detected by adding al2o3 nps to the b. subtilis fermentation broth. physiological ... | 2016 | 27440502 |
| breaking down polystyrene through the application of a two-step thermal degradation and bacterial method to produce usable byproducts. | in this work, the objective is to examine a novel approach on the biodegradability of polystyrene is examined. a two-step method for the degradation of polystyrene to chemically useful products has been devised. initially, polystyrene is liquefied via a thermal degradation procedure predominantly into styrene monomers at low temperatures and left to cool down. application of microorganisms in a second step targets the breaking of the polymer into smaller organic molecules. microorganisms tested ... | 2017 | 27440221 |
| unknown unknowns: essential genes in quest for function. | the experimental design of a minimal synthetic genome revealed the presence of a large number of genes without ascribed function, in part because the abstract laws of life must be implemented within ad hoc material contraptions. creating a function needs recruitment of some pre-existing structure and this reveals kludges in their set-up and history. here, we show that looking for functions as an engineer would help in discovery of a significant number of those, proposed together with conceptual ... | 2016 | 27435445 |
| inter-sigmulon communication through topological promoter coupling. | divergent transcription from within bacterial intergenic regions frequently involves promoters dependent on alternative σ-factors. this is the case for the non-overlapping σ(70)- and σ(54)-dependent promoters that control production of the substrate-responsive regulator and enzymes for (methyl)phenol catabolism. here, using an array of in vivo and in vitro assays, we identify transcription-driven supercoiling arising from the σ(54)-promoter as the mechanism underlying inter-promoter communicatio ... | 2016 | 27422872 |
| self-regulation and interplay of rsm family proteins modulate the lifestyle of pseudomonas putida. | in the plant-beneficial bacterium pseudomonas putida kt2440, three genes have been identified that encode posttranscriptional regulators of the csra/rsma family. their regulatory roles in the motile and sessile lifestyles of p. putida have been investigated by generating single-, double-, and triple-null mutants and by overexpressing each protein (rsma, rsme, and rsmi) in different genetic backgrounds. the rsm triple mutant shows reduced swimming and swarming motilities and increased biofilm for ... | 2016 | 27422830 |
| chemical compositions and antibacterial activity of the leaf and stem oils of piper porphyrophyllum (lindl.) n.e. br. | the essential oils obtained by hydrodistillation from the fresh leaf and stem of piper porphyrophyllum n.e. br. were analyzed by gc and gc/ms. thirty four constituents were identified in the leaf oil, while thirty eight constituents were identified in the stems oil. the most abundant components in the leaf oil included bicyclogermacrene (14.7 %), α-copaene (13.2 %) and β-phellandrene (9.5 %) while sabinene (15.5 %), bicyclogermacrene (12.3 %) and α-copaene (8.1 %) were the main constituents in t ... | 2012 | 27418915 |
| engineering pseudomonas putida kt2440 for simultaneous degradation of carbofuran and chlorpyrifos. | currently, chlorpyrifos (cp) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. some cost-effective techniques are required for the remediation of combined pollution caused by multiple pesticides. in this work, we aim at constructing a detectable recombinant microorganism with the capacity to simultaneously degrade cp and carbofuran. to achieve this purpose, cp/carbofur ... | 2016 | 27418102 |
| pseudomonas putida as a platform for the synthesis of aromatic compounds. | aromatic compounds such as l-phenylalanine, 2-phenylethanol and trans-cinnamate are aromatic compounds of industrial interest. current trends support replacement of chemical synthesis of these compounds by 'green' alternatives produced in microbial cell factories. the solvent-tolerant pseudomonas putida dot-t1e strain was genetically modified to produce up to 1 g l-1 of l-phenylalanine. in order to engineer this strain, we carried out the following stepwise process: (1) we selected random mutant ... | 2016 | 27417954 |
| draft genome sequence of pseudomonas putida cbf10-2, a soil isolate with bioremediation potential in agricultural and industrial environmental settings. | pseudomonas putida cbf10-2 is a microorganism isolated from farmland soil in fairchild, tx, found to degrade high-impact xenobiotics, including organophosphate insecticides, petroleum hydrocarbons, and both monocyclic and polycyclic aromatics. the versatility of cbf10-2 makes it useful for multipurpose bioremediation of contaminated sites in agricultural and industrial environments. | 2016 | 27417844 |
| arabidopsis thaliana contains both ni2+ and zn2+ dependent glyoxalase i enzymes and ectopic expression of the latter contributes more towards abiotic stress tolerance in e. coli. | the glyoxalase pathway is ubiquitously found in all the organisms ranging from prokaryotes to eukaryotes. it acts as a major pathway for detoxification of methylglyoxal (mg), which deleteriously affects the biological system in stress conditions. the first important enzyme of this system is glyoxalase i (glyi). it is a metalloenzyme which requires divalent metal ions for its activity. this divalent metal ion can be either zn2+ as found in most of eukaryotes or ni2+ as seen in prokaryotes. in the ... | 2016 | 27415831 |
| evaluation of the catalytic contribution from a positioned general base in ketosteroid isomerase. | proton transfer reactions are ubiquitous in enzymes and utilize active site residues as general acids and bases. crystal structures and site-directed mutagenesis are routinely used to identify these residues, but assessment of their catalytic contribution remains a major challenge. in principle, effective molarity measurements, in which exogenous acids/bases rescue the reaction in mutants lacking these residues, can estimate these catalytic contributions. however, these exogenous moieties can be ... | 2016 | 27410422 |
| permanent draft genome sequence of sulfoquinovose-degrading pseudomonas putida strain sq1. | pseudomonas putida sq1 was isolated for its ability to utilize the plant sugar sulfoquinovose (6-deoxy-6-sulfoglucose) for growth, in order to define its sq-degradation pathway and the enzymes and genes involved. here we describe the features of the organism, together with its draft genome sequence and annotation. the draft genome comprises 5,328,888 bp and is predicted to encode 5,824 protein-coding genes; the overall g + c content is 61.58 %. the genome annotation is being used for identificat ... | 2015 | 27408681 |
| crystal structure of sgcj, an ntf2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic c-1027. | comparative analysis of the enediyne biosynthetic gene clusters revealed sets of conserved genes serving as outstanding candidates for the enediyne core. here we report the crystal structures of sgcj and its homologue ncs-orf16, together with gene inactivation and site-directed mutagenesis studies, to gain insight into enediyne core biosynthesis. gene inactivation in vivo establishes that sgcj is required for c-1027 production in streptomyces globisporus. sgcj and ncs-orf16 share a common struct ... | 2016 | 27406907 |
| metabolic analysis of the response of pseudomonas putida dot-t1e strains to toluene using fourier transform infrared spectroscopy and gas chromatography mass spectrometry. | an exceptionally interesting stress response of pseudomonas putida strains to toxic substances is the induction of efflux pumps that remove toxic chemical substances from the bacterial cell out to the external environment. to exploit these microorganisms to their full potential a deeper understanding of the interactions between the bacteria and organic solvents is required. thus, this study focuses on investigation of metabolic changes in p. putida upon exposure to toluene. | 2016 | 27398079 |
| impact of soil salinity on the structure of the bacterial endophytic community identified from the roots of caliph medic (medicago truncatula). | in addition to being a forage crop, caliph medic (medicago truncatula) is also a model legume plant and is used for research focusing on the molecular characterization of the interaction between rhizobia and plants. however, the endophytic microbiome in this plant is poorly defined. endophytic bacteria play a role in supplying plants with the basic requirements necessary for growth and development. moreover, these bacteria also play a role in the mechanism of salinity stress adaptation in plants ... | 2016 | 27391592 |
| deterioration of tolerance to hydrophobic organic solvents in a toluene-tolerant strain of pseudomonas putida under the conditions lowering aerobic respiration. | the growth curve (increase in the number of viable cells) of a toluene-tolerant strain pseudomonas putida px51t was not reproducible in the presence of harmful organic solvents, such as p-xylene and toluene. the survival often fluctuated the during late exponential phase of growth. the repetitive growth was obtained by maintaining po2 20-40% (v/v) in the culture flask. however, even under these aerobic conditions, the cells starved for a carbon source were killed by exposure to harmful solvents. ... | 1999 | 27389504 |
| source-sink plasmid transfer dynamics maintain gene mobility in soil bacterial communities. | horizontal gene transfer is a fundamental process in bacterial evolution that can accelerate adaptation via the sharing of genes between lineages. conjugative plasmids are the principal genetic elements mediating the horizontal transfer of genes, both within and between bacterial species. in some species, plasmids are unstable and likely to be lost through purifying selection, but when alternative hosts are available, interspecific plasmid transfer could counteract this and maintain access to pl ... | 2016 | 27385827 |
| biofilm adaptation to iron availability in the presence of biotite and consequences for chemical weathering. | bacteria in nature often live within biofilms, exopolymeric matrices that provide a favorable environment that can differ markedly from their surroundings. biofilms have been found growing on mineral surfaces and are expected to play a role in weathering those surfaces, but a clear understanding of how environmental factors, such as trace-nutrient limitation, influence this role is lacking. here, we examine biofilm development by pseudomonas putida in media either deficient or sufficient in fe d ... | 2016 | 27384343 |
| mcsm-lig: quantifying the effects of mutations on protein-small molecule affinity in genetic disease and emergence of drug resistance. | the ability to predict how a mutation affects ligand binding is an essential step in understanding, anticipating and improving the design of new treatments for drug resistance, and in understanding genetic diseases. here we present mcsm-lig, a structure-guided computational approach for quantifying the effects of single-point missense mutations on affinities of small molecules for proteins. mcsm-lig uses graph-based signatures to represent the wild-type environment of mutations, and small-molecu ... | 2016 | 27384129 |
| comparative genomic and functional analyses: unearthing the diversity and specificity of nematicidal factors in pseudomonas putida strain 1a00316. | we isolated pseudomonas putida (p. putida) strain 1a00316 from antarctica. this bacterium has a high efficiency against meloidogyne incognita (m. incognita) in vitro and under greenhouse conditions. the complete genome of p. putida 1a00316 was sequenced using pacbio single molecule real-time (smrt) technology. a comparative genomic analysis of 16 pseudomonas strains revealed that although p. putida 1a00316 belonged to p. putida, it was phenotypically more similar to nematicidal pseudomonas fluor ... | 2016 | 27384076 |
| occurrence of enterobacteriaceae in raw meat and in human samples from egyptian retail sellers. | the present study was performed to assess the presence of enterobacteriaceae in raw meat and handlers in egypt using cultivation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms). a total of 100 raw meat samples (chicken and beef meat, 50 each) were randomly purchased from butchers and local meat retailers located at mansoura city, egypt. fifty human samples were collected from meat handlers (hand swabs and stool specimens, 25 each). 228 bacterial is ... | 2014 | 27379312 |
| current technological improvements in enzymes toward their biotechnological applications. | enzymes from extremophiles are creating interest among researchers due to their unique properties and the enormous power of catalysis at extreme conditions. since community demands are getting more intensified, therefore, researchers are applying various approaches viz. metagenomics to increase the database of extremophilic species. furthermore, the innovations are being made in the naturally occurring enzymes utilizing various tools of recombinant dna technology and protein engineering, which a ... | 2016 | 27379087 |
| relationships between the regulatory systems of quorum sensing and multidrug resistance. | cell-cell communications, known as quorum sensing (qs) in bacteria, involve the signal molecules as chemical languages and the corresponding receptors as transcriptional regulators. in gram-negative bacteria, orphan luxr receptors recognize signals more than just acylhomoserine lactones, and modulate interspecies and interkingdom communications. whereas, in the gram-positive streptomyces, pseudo gamma-butyrolactones (gbls) receptors bind antibiotics other than gbl signals, and coordinate antibio ... | 2016 | 27379084 |
| development of genetic tools for the manipulation of the planctomycetes. | bacteria belonging to the planctomycetes, verrucomicrobia, chlamydiae (pvc) superphylum are of interest for biotechnology, evolutionary cell biology, ecology, and human health. some pvc species lack a number of typical bacterial features while others possess characteristics that are usually more associated to eukaryotes or archaea. for example, the planctomycetes phylum is atypical for the absence of the ftsz protein and for the presence of a developed endomembrane system. studies of the cellula ... | 2016 | 27379046 |
| poly-β-hydroxybutyrate metabolism is unrelated to the sporulation and parasporal crystal protein formation in bacillus thuringiensis. | poly-3-hydroxybutyrate (phb) is a natural polymer synthesized by many bacteria as a carbon-energy storage material. it was accumulated maximally prior to the spore formation but was degraded during the process of sporulation in bacillus thuringiensis. intriguingly, b. thuringiensis also accumulates large amounts of insecticidal crystal proteins (icps) during sporulation, which requires considerable input of carbon and energy sources. how phb accumulation affects sporulation and icp formation rem ... | 2016 | 27379025 |
| development of formaldehyde biosensor for determination of formalin in fish samples; malabar red snapper (lutjanus malabaricus) and longtail tuna (thunnus tonggol). | electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. during bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an electrochemical detector. a formaldehyde biosensor was successfully developed by depositing an ionic liquid (il) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([emim][otf])), gold nano ... | 2016 | 27376338 |
| bacteriophages as weapons against bacterial biofilms in the food industry. | microbiological contamination in the food industry is often attributed to the presence of biofilms in processing plants. bacterial biofilms are complex communities of bacteria attached to a surface and surrounded by an extracellular polymeric material. their extreme resistance to cleaning and disinfecting processes is related to a unique organization, which implies a differential bacterial growth and gene expression inside the biofilm. the impact of biofilms on health, and the economic consequen ... | 2016 | 27375566 |
| role of conserved met112 residue in the catalytic activity and stability of ketosteroid isomerase. | ketosteroid isomerase (3-oxosteroid δ(5)-δ(4)-isomerase, ksi) from pseudomonas putida catalyzes allylic rearrangement of the 5,6-double bond of δ(5)-3-ketosteroid to 4,5-position by stereospecific intramolecular transfer of a proton. the active site of ksi is formed by several hydrophobic residues and three catalytic residues (tyr14, asp38, and asp99). in this study, we investigated the role of a hydrophobic met112 residue near the active site in the catalysis, steroid binding, and stability of ... | 2016 | 27375051 |
| disk carbapenemase test for the rapid detection of kpc-, ndm-, and other metallo-β-lactamase-producing gram-negative bacilli. | rapid detection of carbapenemase-producing gram-negative bacilli (gnb) is required for optimal treatment of infected patients. we developed and assessed a new disk carbapenemase test (dct). | 2016 | 27374708 |
| 3-fluorophenmetrazine, a fluorinated analogue of phenmetrazine: studies on in vivo metabolism in rat and human, in vitro metabolism in human cyp isoenzymes and microbial biotransformation in pseudomonas putida and wastewater using gc and lc coupled to (hr)-ms techniques. | wastewater-based epidemiology (wbe) as means to estimate illicit drug and new psychoactive substance (nps) consumption with spatial and temporal resolution is gaining increasing attention. in order to evaluate a given nps using wbe, in vivo metabolism and microbial biotransformation of excretion products and unchanged compounds need evaluation. the aims of this study were to identify in vivo phase i and ii metabolites of the nps 3-fluorophenmetrazine (3-fpm) in human and rat urine and study the ... | 2016 | 27372653 |
| genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms. | hyaluronic acid, or ha, is a rigid and linear biopolymer belonging to the class of the glycosaminoglycans, and composed of repeating units of the monosaccharides glucuronic acid and n-acetylglucosamine. ha has multiple important functions in the human body, due to its properties such as bio-compatibility, lubricity and hydrophilicity, it is widely applied in the biomedical, food, health and cosmetic fields. the growing interest in this molecule has motivated the discovery of new ways of obtainin ... | 2016 | 27370777 |
| the ssr protein (t1e_1405) from pseudomonas putida dot-t1e enables oligonucleotide-based recombineering in platform strain p. putida em42. | some strains of the soil bacterium pseudomonas putida have become in recent years platforms of choice for hosting biotransformations of industrial interest. despite availability of many genetic tools for this microorganism, genomic editing of the cell factory p. putida em42 (a derivative of reference strain kt2440) is still a time-consuming endeavor. in this work we have investigated the in vivo activity of the ssr protein encoded by the open reading frame t1e_1405 from pseudomonas putida dot-t1 ... | 2016 | 27367544 |
| methane-oxidizing enzymes: an upstream problem in biological gas-to-liquids conversion. | biological conversion of natural gas to liquids (bio-gtl) represents an immense economic opportunity. in nature, aerobic methanotrophic bacteria and anaerobic archaea are able to selectively oxidize methane using methane monooxygenase (mmo) and methyl coenzyme m reductase (mcr) enzymes. although significant progress has been made toward genetically manipulating these organisms for biotechnological applications, the enzymes themselves are slow, complex, and not recombinantly tractable in traditio ... | 2016 | 27366961 |
| a de-novo genome analysis pipeline (denogap) for large-scale comparative prokaryotic genomics studies. | comparative analysis of whole genome sequence data from closely related prokaryotic species or strains is becoming an increasingly important and accessible approach for addressing both fundamental and applied biological questions. while there are number of excellent tools developed for performing this task, most scale poorly when faced with hundreds of genome sequences, and many require extensive manual curation. | 2016 | 27363390 |
| stereochemical consequences of vinylpyruvate hydratase-catalyzed reactions. | a stereochemical analysis has been carried out on two vinylpyruvate hydratases (vph), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4s-hydroxypentanoate in meta-fission pathways. bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. the analysis was carried out using the 5-methyl and 5-chloro derivatives of 2-hydroxy-2,4-pentadienoate with the enzymes from pseudomonas putida mt-2 (pp) and leptothrix cholodnii sp-6 (lc). in both organisms, vph ... | 2016 | 27362840 |
| bacteria in nanoparticle synthesis: current status and future prospects. | microbial metal reduction can be a strategy for remediation of metal contaminations and wastes. bacteria are capable of mobilization and immobilization of metals and in some cases, the bacteria which can reduce metal ions show the ability to precipitate metals at nanometer scale. biosynthesis of nanoparticles (nps) using bacteria has emerged as rapidly developing research area in green nanotechnology across the globe with various biological entities being employed in synthesis of nps constantly ... | 2014 | 27355054 |
| environmental factors modulating the stability and enzymatic activity of the petrotoga mobilis esterase (pmest). | enzymes isolated from thermophilic organisms found in oil reservoirs can find applications in many fields, including the oleochemical, pharmaceutical, bioenergy, and food/dairy industries. in this study, in silico identification and recombinant production of an esterase from the extremophile bacteria petrotoga mobilis (designated pmest) were performed. then biochemical, bioinformatics and structural characterizations were undertaken using a combination of synchrotron radiation circular dichroism ... | 2016 | 27351338 |
| pyridine nucleotide transhydrogenases enable redox balance of pseudomonas putida during biodegradation of aromatic compounds. | the metabolic versatility of the soil bacterium pseudomonas putida is reflected by its ability to execute strong redox reactions (e.g., mono- and di-oxygenations) on aromatic substrates. biodegradation of aromatics occurs via the pathway encoded in the archetypal tol plasmid pww0, yet the effect of running such oxidative route on redox balance against the background metabolism of p. putida remains unexplored. to answer this question, the activity of pyridine nucleotide transhydrogenases (that ca ... | 2016 | 27348295 |
| l-2-haloacid dehalogenase (dehl) from rhizobium sp. rc1. | l-2-haloacid dehalogenase (dehl) from rhizobium sp. rc1 is a stereospecific enzyme that acts exclusively on l-isomers of 2-chloropropionate and dichloroacetate. the amino acid sequence of this enzyme is substantially different from those of other l-specific dehalogenases produced by other organisms. dehl has not been crystallised, and hence its three-dimensional structure is unavailable. herein, we review what is known concerning dehl and tentatively identify the amino acid residues important fo ... | 2016 | 27347470 |
| pseudomonas sp. lz-q continuously degrades phenanthrene under hypersaline and hyperalkaline condition in a membrane bioreactor system. | 2015 | 27340693 | |
| pseudomonas sp. lz-q continuously degrades phenanthrene under hypersaline and hyperalkaline condition in a membrane bioreactor system. | 2015 | 27340693 | |
| biosynthesis of α-pyrones. | the α-pyrone moiety is a structural feature found in a huge variety of biologically active metabolites. in recent times new insights into additional biosynthetic mechanisms, yielding in such six-membered unsaturated ester ring residues have been obtained. the purpose of this mini-review is to give a brief overview of α-pyrones and the mechanisms forming the basis of their natural synthesis. especially the chain interconnecting enzymes, showing homology to ketosynthases which catalyze claisen-lik ... | 2016 | 27340449 |
| draft genome sequence of comamonas thiooxydans strain phe2-6 (nbrc 110656), a chlorinated-ethene-degrading bacterium. | comamonas thiooxydans strain phe2-6 (nbrc 110656), which was isolated from a trichloroethene-contaminated site in japan, utilizes phenol as a sole source of carbon and cometabolizes cis- and trans-dichloroethenes. we report here the draft genome sequence of this strain, containing 5,309,680 bp, with 60.6% g+c content. | 2016 | 27340052 |
| acidobacteria strains from subdivision 1 act as plant growth-promoting bacteria. | acidobacteria is one of the most abundant phyla in soils and has been detected in rhizosphere mainly based on cultivation-independent approaches such as 16s rrna gene survey. although putative interaction of acidobacteria with plants was suggested, so far no plant-bacterial interactions were shown. therefore, we performed several in vitro tests to evaluate acidobacteria-plant interactions and the possible mechanisms involved in such interaction. we observed that arabidopsis thaliana inoculated w ... | 2016 | 27339258 |
| crystal structures of the global regulator dasr from streptomyces coelicolor: implications for the allosteric regulation of gntr/hutc repressors. | small molecule effectors regulate gene transcription in bacteria by altering the dna-binding affinities of specific repressor proteins. although the gntr proteins represent a large family of bacterial repressors, only little is known about the allosteric mechanism that enables their function. dasr from streptomyces coelicolor belongs to the gntr/hutc subfamily and specifically recognises operators termed dasr-responsive elements (dre-sites). its dna-binding properties are modulated by phosphoryl ... | 2016 | 27337024 |
| novel polyhydroxyalkanoate copolymers produced in pseudomonas putida by metagenomic polyhydroxyalkanoate synthases. | bacterially produced biodegradable polyhydroxyalkanoates (phas) with versatile properties can be achieved using different pha synthases (phacs). this work aims to expand the diversity of known phacs via functional metagenomics and demonstrates the use of these novel enzymes in pha production. complementation of a pha synthesis-deficient pseudomonas putida strain with a soil metagenomic cosmid library retrieved 27 clones expressing either class i, class ii, or unclassified pha synthases, and many ... | 2016 | 27333909 |
| metabolomics analysis reveals the participation of efflux pumps and ornithine in the response of pseudomonas putida dot-t1e cells to challenge with propranolol. | efflux pumps are critically important membrane components that play a crucial role in strain tolerance in pseudomonas putida to antibiotics and aromatic hydrocarbons that result in these toxicants being expelled from the bacteria. here, the effect of propranolol on p. putida was examined by sudden addition of 0.2, 0.4 and 0.6 mg ml-1 of this β-blocker to several strains of p. putida, including the wild type dot-t1e and the efflux pump knockout mutants dot-t1e-ps28 and dot-t1e-18. bacterial viabi ... | 2016 | 27331395 |
| whole genome sequence analysis of an alachlor and endosulfan degrading pseudomonas strain w15feb9b isolated from ochlockonee river, florida. | we recently isolated a pseudomonas sp. strain w15feb9b from ochlockonee river, florida and demonstrated potent biodegradative activity against two commonly used pesticides - alachlor [(2-chloro-2',6'-diethylphenyl-n (methoxymethyl)acetanilide)] and endosulfan [(6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9methano-2,3,4-benzo(e)di-oxathiepin-3-oxide], respectively. to further identify the repertoire of metabolic functions possessed by strain w15feb9b, a draft genome sequence was obtained, ... | 2016 | 27330991 |
| staphylococcus aureus coordinates leukocidin expression and pathogenesis by sensing metabolic fluxes via rpirc. | staphylococcus aureus is a formidable human pathogen that uses secreted cytolytic factors to injure immune cells and promote infection of its host. of these proteins, the bicomponent family of pore-forming leukocidins play critical roles in s. aureus pathogenesis. the regulatory mechanisms governing the expression of these toxins are incompletely defined. in this work, we performed a screen to identify transcriptional regulators involved in leukocidin expression in s. aureus strain usa300. we di ... | 2016 | 27329753 |
| the induction and identification of novel colistin resistance mutations in acinetobacter baumannii and their implications. | acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. multidrug resistant a. baumannii has risen rapidly in vietnam, where colistin is becoming the drug of last resort for many infections. in this study we generated spontaneous colistin resistant progeny (up to >256 μg/μl) from four colistin susceptible vietnamese isolates and one susceptible refere ... | 2016 | 27329501 |
| phosphorus mobilizing consortium mammoth p(™) enhances plant growth. | phosphorus (p) is a critical nutrient used to maximize plant growth and yield. current agriculture management practices commonly experience low plant p use efficiency due to natural chemical sorption and transformations when p fertilizer is applied to soils. a perplexing challenge facing agriculture production is finding sustainable solutions to deliver p more efficiently to plants. using prescribed applications of specific soil microbial assemblages to mobilize soil bound-p to improve crop nutr ... | 2016 | 27326379 |
| genomics of high molecular weight plasmids isolated from an on-farm biopurification system. | the use of biopurification systems (bps) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. bps environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (mges), which play a key role in bacterial adaptation and evolution in such environments. here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-fa ... | 2016 | 27321040 |
| in silico analysis for prediction of degradative capacity of pseudomonas putida sf1. | the study employs draft genome sequence data to explore p-nitrophenol (pnp) degradation activity of pseudomonas putida strain sf-1 at a genomic scale. annotation analysis proposes that the strain sf1 not only possesses the gene cluster for pnp utilization but also for the utilization of benzoate, catechol, hydroxybenzoate, protocatechuate, and homogentisate. further, the analysis was carried out to understand more details of pnp 4-monooxygenase and its regulator. a comparative analysis of pnp 4- ... | 2016 | 27317892 |
| listeria monocytogenes dna glycosylase adlp affects flagellar motility, biofilm formation, virulence, and stress responses. | the temperature-dependent alteration of flagellar motility gene expression is critical for the foodborne pathogen listeria monocytogenes to respond to a changing environment. in this study, a genetic determinant, l. monocytogenes f2365_0220 (lmof2365_0220), encoding a putative protein that is structurally similar to the bacillus cereus alkyl base dna glycosylase (alkd), was identified. this determinant was involved in the transcriptional repression of flagellar motility genes and was named adlp ... | 2016 | 27316964 |
| characterization of quinohemoprotein amine dehydrogenase from pseudomonas putida. | quinohemoprotein amine dehydrogenase (amdh) was purified and crystallized from the soluble fraction of pseudomonas putida ifo 15366 grown on n-butylamine medium. amdh gave a single component in analytical ultracentrifugation showing an intrinsic sedimentation coefficient of 5.8s. amdh showed a typical absorption spectrum of cytochrome c showing maxima at 554, 522, 420, and 320 nm in the reduced form and one peak at 410 nm, a shoulder at 350 nm, and a broad hill around 530 nm in the oxidized form ... | 1998 | 27315927 |
| integrated analysis of root microbiomes of soybean and wheat from agricultural fields. | root associated bacteria are critical for plant growth and health. understanding the composition and role of root microbiota is crucial toward agricultural practices that are less dependent on chemical fertilization, which has known negative effects on the environment and human health. here we analyzed the root-associated microbiomes of soybean and wheat under agricultural field conditions. we took samples from 11 different production fields across a large geographic area. we used 16s rrna pyros ... | 2016 | 27312589 |
| engineering of baeyer-villiger monooxygenase-based escherichia coli biocatalyst for large scale biotransformation of ricinoleic acid into (z)-11-(heptanoyloxy)undec-9-enoic acid. | baeyer-villiger monooxygenases (bvmos) are able to catalyze regiospecific baeyer-villiger oxygenation of a variety of cyclic and linear ketones to generate the corresponding lactones and esters, respectively. however, the enzymes are usually difficult to express in a functional form in microbial cells and are rather unstable under process conditions hindering their large-scale applications. thereby, we investigated engineering of the bvmo from pseudomonas putida kt2440 and the gene expression sy ... | 2016 | 27311560 |
| gcsr, a tyrr-like enhancer-binding protein, regulates expression of the glycine cleavage system in pseudomonas aeruginosa pao1. | glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (gcs). our lab previously identified the transcriptional regulator gcsr (pa2449) as being required for catabolism of glycine in the opportunistic pathogen pseudomonas aeruginosa pao1. in an effort to clarify and have an overall better understanding of the role of gcsr ... | 2016 | 27303730 |
| when genome-based approach meets the "old but good": revealing genes involved in the antibacterial activity of pseudomonas sp. p482 against soft rot pathogens. | dickeya solani and pectobacterium carotovorum subsp. brasiliense are recently established species of bacterial plant pathogens causing black leg and soft rot of many vegetables and ornamental plants. pseudomonas sp. strain p482 inhibits the growth of these pathogens, a desired trait considering the limited measures to combat these diseases. in this study, we determined the genetic background of the antibacterial activity of p482, and established the phylogenetic position of this strain. pseudomo ... | 2016 | 27303376 |
| biological control of rice bakanae by an endophytic bacillus oryzicola yc7007. | in our previous study, we reported that a novel endophytic bacterium bacillus oryzicola yc7007 has suppressed bacterial diseases of rice via induced systemic resistance and antibiotic production. this endophytic strain, b. oryzicola yc7007 was used as a biological control agent against bakanae disease of rice caused by fusarium fujikuroi, and its mechanism of interaction with the pathogen and the rice was further elucidated. root drenching with b. oryzicola yc7007 suspension reduced the disease ... | 2016 | 27298598 |
| meta-analysis reveals that the genus pseudomonas can be a better choice of biological control agent against bacterial wilt disease caused by ralstonia solanacearum. | biological control agents (bcas) from different microbial taxa are increasingly used to control bacterial wilt caused by ralstonia solanacearum. however, a quantitative research synthesis has not been conducted on the role of bcas in disease suppression. therefore, the present study aimed to meta-analyze the impacts of bcas on both ralstonia wilt disease suppression and plant (host) growth promotion. the analysis showed that the extent of disease suppression by bcas varied widely among studies, ... | 2016 | 27298597 |
| highly regio- and enantioselective multiple oxy- and amino-functionalizations of alkenes by modular cascade biocatalysis. | new types of asymmetric functionalizations of alkenes are highly desirable for chemical synthesis. here, we develop three novel types of regio- and enantioselective multiple oxy- and amino-functionalizations of terminal alkenes via cascade biocatalysis to produce chiral α-hydroxy acids, 1,2-amino alcohols and α-amino acids, respectively. basic enzyme modules 1-4 are developed to convert alkenes to (s)-1,2-diols, (s)-1,2-diols to (s)-α-hydroxyacids, (s)-1,2-diols to (s)-aminoalcohols and (s)-α-hy ... | 2016 | 27297777 |
| isolation of bdellovibrio sp. from soil samples in mexico and their potential applications in control of pathogens. | in this study, two strains of bdellovibrio were isolated from soil samples using the culture-dependent technique and two members of the family enterobacteriaceae (klebsiella sp. and salmonella sp.) as prey. the bdellovibrio strains were bacteriolytic, plaque-forming, and highly motile gram-negative bacteria. we identified and confirmed the bdellovibrio strains using microscopy, pcr amplification, and sequencing of the 16s rrna gene. they were observed to be different strains based on hit locus a ... | 2016 | 27297185 |
| potential of biological agents in decontamination of agricultural soil. | pesticides are widely used for the control of weeds, diseases, and pests of cultivated plants all over the world, mainly since the period after the second world war. the use of pesticides is very extensive to control harm of pests all over the globe. persistent nature of most of the synthetic pesticides causes serious environmental concerns. decontamination of these hazardous chemicals is very essential. this review paper elaborates the potential of various biological agents in decontamination o ... | 2016 | 27293964 |
| signaling states of a short blue-light photoreceptor protein ppsb1-lov revealed from crystal structures and solution nmr spectroscopy. | light-oxygen-voltage (lov) domains represent the photo-responsive domains of various blue-light photoreceptor proteins and are widely distributed in plants, algae, fungi, and bacteria. here, we report the dark-state crystal structure of ppsb1-lov, a slow-reverting short lov protein from pseudomonas putida that is remarkably different from our previously published "fully light-adapted" structure [1]. a direct comparison of the two structures provides insight into the light-activated signaling mec ... | 2016 | 27291287 |