Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| structural and biochemical characterization of a metagenome-derived esterase with a long n-terminal extension. | the genes encoding six novel esterolytic/lipolytic enzymes, termed lc-est1∼6, were isolated from a fosmid library of a leaf-branch compost metagenome by functional screening using tributyrin agar plates. these enzymes greatly vary in size and amino acid sequence. the highest identity between the amino acid sequence of each enzyme and that available from the database varies from 44 to 73%. of these metagenome-derived enzymes, lc-est1 is characterized by the presence of a long n-terminal extension ... | 2014 | 25348365 |
| structural and biochemical characterization of a metagenome-derived esterase with a long n-terminal extension. | the genes encoding six novel esterolytic/lipolytic enzymes, termed lc-est1∼6, were isolated from a fosmid library of a leaf-branch compost metagenome by functional screening using tributyrin agar plates. these enzymes greatly vary in size and amino acid sequence. the highest identity between the amino acid sequence of each enzyme and that available from the database varies from 44 to 73%. of these metagenome-derived enzymes, lc-est1 is characterized by the presence of a long n-terminal extension ... | 2014 | 25348365 |
| nutrition effects on the biofilm immobilization and 3,5-dnba degradation of comamonas testosteroni a3 during bioaugmentation treatment. | the survival of inoculated microbes is critical for successful bioaugmentation in wastewater treatment. the influence of readily available nutrients (rans) on the colonization of two functional bacteria, pseudomonas putida m9, a strong biofilm-forming strain, and comamonas testosteroni a3, a 3,5-dinitrobenzoic acid (3,5-dnba)-degrading strain, in biofilms was studied with 3,5-dinitrobenoic acid synthetic wastewater (dcmm) complemented with various ratios of luria-bertani broth (lb). with the inc ... | 2016 | 25345550 |
| molecular mechanism of nicotine degradation by a newly isolated strain, ochrobactrum sp. strain sjy1. | a newly isolated strain, sjy1, identified as ochrobactrum sp., utilizes nicotine as a sole source of carbon, nitrogen, and energy. strain sjy1 could efficiently degrade nicotine via a variant of the pyridine and pyrrolidine pathways (the vpp pathway), which highlights bacterial metabolic diversity in relation to nicotine degradation. a 97-kbp dna fragment containing six nicotine degradation-related genes was obtained by gap closing from the genome sequence of strain sjy1. three genes, designated ... | 2014 | 25344232 |
| molecular mechanism of nicotine degradation by a newly isolated strain, ochrobactrum sp. strain sjy1. | a newly isolated strain, sjy1, identified as ochrobactrum sp., utilizes nicotine as a sole source of carbon, nitrogen, and energy. strain sjy1 could efficiently degrade nicotine via a variant of the pyridine and pyrrolidine pathways (the vpp pathway), which highlights bacterial metabolic diversity in relation to nicotine degradation. a 97-kbp dna fragment containing six nicotine degradation-related genes was obtained by gap closing from the genome sequence of strain sjy1. three genes, designated ... | 2014 | 25344232 |
| emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management. | atypical and multidrug resistance, especially esbl and carbapenemase expressing enterobacteriaceae, is globally spreading. therefore, it becomes increasingly difficult to achieve therapeutic success by calculated antibiotic therapy. consequently, rapid antibiotic resistance testing is essential. various molecular and mass spectrometry-based approaches have been introduced in diagnostic microbiology to speed up the providing of reliable resistance data. pcr- and sequencing-based approaches are th ... | 2014 | 25343142 |
| chromobacterium csp_p reduces malaria and dengue infection in vector mosquitoes and has entomopathogenic and in vitro anti-pathogen activities. | plasmodium and dengue virus, the causative agents of the two most devastating vector-borne diseases, malaria and dengue, are transmitted by the two most important mosquito vectors, anopheles gambiae and aedes aegypti, respectively. insect-bacteria associations have been shown to influence vector competence for human pathogens through multi-faceted actions that include the elicitation of the insect immune system, pathogen sequestration by microbes, and bacteria-produced anti-pathogenic factors. t ... | 2014 | 25340821 |
| in vitro fermentation of lactulose by human gut bacteria. | lactulose has been known as a prebiotic that can selectively stimulate the growth of beneficial bifidobacteria and lactobacilli. recent studies have indicated that streptococcus mutans, clostridium perfringens, and faecalibacterium prausnitzii are also able to utilize lactulose. however, the previous studies mainly focused on the utilization of lactulose by individual strains, and few studies were designed to identify the species that could utilize lactulose among gut microbiota. this study aime ... | 2014 | 25340538 |
| bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality. | some previous studies have identified bacteria in semen as being a potential factor in male infertility. however, only few types of bacteria were taken into consideration while using pcr-based or culturing methods. here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. ninety-six semen samples collected were examined for bacterial communities, measuring seven cli ... | 2014 | 25340531 |
| high-throughput screening of dipeptide utilization mediated by the abc transporter dppbcdf and its substrate-binding proteins dppa1-a5 in pseudomonas aeruginosa. | in this study, we show that the dppbcdf operon of pseudomonas aeruginosa pa14 encodes an abc transporter responsible for the utilization of di/tripeptides. the substrate specificity of abc transporters is determined by its associated substrate-binding proteins (sbps). whereas in e. coli only one protein, dppa, determines the specificity of the transporter, five orthologous sbps, dppa1-a5 are present in p. aeruginosa. multiple sbps might broaden the substrate specificity by increasing the transpo ... | 2014 | 25338022 |
| broad host range plasmids can invade an unexpectedly diverse fraction of a soil bacterial community. | conjugal plasmids can provide microbes with full complements of new genes and constitute potent vehicles for horizontal gene transfer. conjugal plasmid transfer is deemed responsible for the rapid spread of antibiotic resistance among microbes. while broad host range plasmids are known to transfer to diverse hosts in pure culture, the extent of their ability to transfer in the complex bacterial communities present in most habitats has not been comprehensively studied. here, we isolated and chara ... | 2014 | 25333461 |
| broad host range plasmids can invade an unexpectedly diverse fraction of a soil bacterial community. | conjugal plasmids can provide microbes with full complements of new genes and constitute potent vehicles for horizontal gene transfer. conjugal plasmid transfer is deemed responsible for the rapid spread of antibiotic resistance among microbes. while broad host range plasmids are known to transfer to diverse hosts in pure culture, the extent of their ability to transfer in the complex bacterial communities present in most habitats has not been comprehensively studied. here, we isolated and chara ... | 2014 | 25333461 |
| application of a novel alkali-tolerant thermostable dyp-type peroxidase from saccharomonospora viridis dsm 43017 in biobleaching of eucalyptus kraft pulp. | saccharomonospora viridis is a thermophilic actinomycete that may have biotechnological applications because of its dye decolorizing activity, though the enzymatic oxidative system responsible for this activity remains elusive. bioinformatic analysis revealed a dyp-type peroxidase gene in the genome of s. viridis dsm 43017 with sequence similarity to peroxidase from dye-decolorizing microbes. this gene, svidyp, consists of 1,215 bp encoding a polypeptide of 404 amino acids. the gene encoding svi ... | 2014 | 25333297 |
| formulation of microbial cocktails for btex biodegradation. | btex biodegradation by a mixed community of micro-organisms offers a promising approach in terms of cost-effectiveness and elimination of secondary pollution. two bacterial strains, pseudomonas putida f1 and pseudomonas stutzeri ox1 were chosen to formulate synthetic consortia based on their ability to biodegrade the mono-aromatic compounds. benzene and toluene supported the growth of both the strains; while ethyl benzene and o-xylene were only utilized as growth substrates by p. putida f1 and p ... | 2015 | 25331771 |
| (r,s)-tetrahydropapaveroline production by stepwise fermentation using engineered escherichia coli. | tetrahydropapaveroline (thp), a benzylisoquinoline alkaloid (bia) found in diverse pharmaceutical compounds, is used as a starting material for the production of bia. thp also has various neurobiological properties but is difficult to synthesize. therefore, a simple method for thp production is desired. recent studies have shown that microbes, especially bacteria, can serve as platforms for synthesizing these complex compounds; however, because bacteria lack organelles, the designed synthetic pa ... | 2014 | 25331563 |
| the diguanylate cyclase gcba facilitates pseudomonas aeruginosa biofilm dispersion by activating bdla. | biofilm dispersion is a highly regulated process that allows biofilm bacteria to respond to changing environmental conditions and to disseminate to new locations. the dispersion of biofilms formed by the opportunistic pathogen pseudomonas aeruginosa is known to require a number of cyclic di-gmp (c-di-gmp)-degrading phosphodiesterases (pdes) and the chemosensory protein bdla, with bdla playing a pivotal role in regulating pde activity and enabling dispersion in response to a wide array of cues. b ... | 2014 | 25331436 |
| the diguanylate cyclase gcba facilitates pseudomonas aeruginosa biofilm dispersion by activating bdla. | biofilm dispersion is a highly regulated process that allows biofilm bacteria to respond to changing environmental conditions and to disseminate to new locations. the dispersion of biofilms formed by the opportunistic pathogen pseudomonas aeruginosa is known to require a number of cyclic di-gmp (c-di-gmp)-degrading phosphodiesterases (pdes) and the chemosensory protein bdla, with bdla playing a pivotal role in regulating pde activity and enabling dispersion in response to a wide array of cues. b ... | 2014 | 25331436 |
| loss of flil alters proteus mirabilis surface sensing and temperature-dependent swarming. | proteus mirabilis is a dimorphic motile bacterium well known for its flagellum-dependent swarming motility over surfaces. in liquid, p. mirabilis cells are 1.5- to 2.0-μm swimmer cells with 4 to 6 flagella. when p. mirabilis encounters a solid surface, where flagellar rotation is limited, swimmer cells differentiate into elongated (10- to 80-μm), highly flagellated swarmer cells. in order for p. mirabilis to swarm, it first needs to detect a surface. the ubiquitous but functionally enigmatic fla ... | 2014 | 25331431 |
| loss of flil alters proteus mirabilis surface sensing and temperature-dependent swarming. | proteus mirabilis is a dimorphic motile bacterium well known for its flagellum-dependent swarming motility over surfaces. in liquid, p. mirabilis cells are 1.5- to 2.0-μm swimmer cells with 4 to 6 flagella. when p. mirabilis encounters a solid surface, where flagellar rotation is limited, swimmer cells differentiate into elongated (10- to 80-μm), highly flagellated swarmer cells. in order for p. mirabilis to swarm, it first needs to detect a surface. the ubiquitous but functionally enigmatic fla ... | 2014 | 25331431 |
| involvement of a 1-cys peroxiredoxin in bacterial virulence. | the killing of bacterial pathogens by macrophages occurs via the oxidative burst and bacteria have evolved to overcome this challenge and survive, using several virulence and defense strategies, including antioxidant mechanisms. we show here that the 1-cys peroxiredoxin lsfa from the opportunistic pathogen pseudomonas aeruginosa is endowed with thiol-dependent peroxidase activity that protects the bacteria from h(2)o(2) and that this protein is implicated in pathogenicity. lsfa belongs to the po ... | 2014 | 25329795 |
| transcriptional activation of multiple operons involved in para-nitrophenol degradation by pseudomonas sp. strain wbc-3. | pseudomonas sp. strain wbc-3 utilizes para-nitrophenol (pnp) as a sole carbon and energy source. the genes involved in pnp degradation are organized in the following three operons: pnpa, pnpb, and pnpcdefg. how the expression of the genes is regulated is unknown. in this study, an lysr-type transcriptional regulator (lttr) is identified to activate the expression of the genes in response to the specific inducer pnp. while the lttr coding gene pnpr was found to be not physically linked to any of ... | 2014 | 25326309 |
| transcriptional activation of multiple operons involved in para-nitrophenol degradation by pseudomonas sp. strain wbc-3. | pseudomonas sp. strain wbc-3 utilizes para-nitrophenol (pnp) as a sole carbon and energy source. the genes involved in pnp degradation are organized in the following three operons: pnpa, pnpb, and pnpcdefg. how the expression of the genes is regulated is unknown. in this study, an lysr-type transcriptional regulator (lttr) is identified to activate the expression of the genes in response to the specific inducer pnp. while the lttr coding gene pnpr was found to be not physically linked to any of ... | 2014 | 25326309 |
| siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen pantoea stewartii subsp. stewartii. | iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. pantoea stewartii subsp. stewartii, the causal agent of stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. the proteins involved in the biosynthesi ... | 2014 | 25326304 |
| siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen pantoea stewartii subsp. stewartii. | iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. pantoea stewartii subsp. stewartii, the causal agent of stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. the proteins involved in the biosynthesi ... | 2014 | 25326304 |
| degradation and changes in toxicity and biodegradability of tetracycline during ozone/ultraviolet-based advanced oxidation. | advanced oxidation processes (aops) composed of o3, h2o2 and ultraviolet (uv) were applied to degrade tetracycline (tc). degradation efficiency was evaluated in terms of changes in absorbance (abs) and total organic carbon (toc). the change in biotoxicity was monitored with escherichia coli and vibrio fischeri. the improvement in biodegradability during oxidation was demonstrated through 5-day biochemical oxygen demand/chemical oxygen demand ratio and aerobic biological treatment. the combinatio ... | 2014 | 25325548 |
| complete genome sequence of n-acylhomoserine lactone-producing pseudomonas sp. strain stflb209, isolated from potato phyllosphere. | pseudomonas sp. strain stflb209 is isolated from the potato leaf and produces n-acylhomoserine lactone quorum-sensing signal compounds. here, we present the 6,332,373-bp complete genome sequence of stflb209, with a g+c content of 60.7%, which carries 5,598 protein-coding genes, 6 rrna operons, and 69 trna genes. | 2014 | 25323715 |
| transcriptomic analysis reveals tomato genes whose expression is induced specifically during effector-triggered immunity and identifies the epk1 protein kinase which is required for the host response to three bacterial effector proteins. | plants have two related immune systems to defend themselves against pathogen attack. initially,pattern-triggered immunity is activated upon recognition of microbe-associated molecular patterns by pattern recognition receptors. pathogenic bacteria deliver effector proteins into the plant cell that interfere with this immune response and promote disease. however, some plants express resistance proteins that detect the presence of specific effectors leading to a robust defense response referred to ... | 2014 | 25323444 |
| isolation and characterization of a nitrile-hydrolysing bacterium isoptericola variabilis rgt01. | a nitrile-hydrolysing bacterium, identified as isoptericola variabilis rgt01, was isolated from industrial effluent through enrichment culture technique using acrylonitrile as the carbon source. whole cells of this microorganism exhibited a broad range of nitrile-hydrolysing activity as they hydrolysed five aliphatic nitriles (acetonitrile, acrylonitrile, propionitrile, butyronitrile and valeronitrile), two aromatic nitriles (benzonitrile and m-tolunitrile) and two arylacetonitriles (4-methoxyph ... | 2014 | 25320428 |
| metabolism dependent chemotaxis of pseudomonas aeruginosa n1 towards anionic detergent sodium dodecyl sulfate. | sodium dodecyl sulfate (sds) is one of the most commonly used detergent, which exhibits excellent biocidal activity against various bacteria and fungi. it is commonly employed in many detergent formulations and is employed for disinfection purposes. it is shown to be toxic to fishes, aquatic animals and is also inhibitory to microbes and cyanobacteria. we had isolated a strain belonging to pseudomonas aeruginosa n1, from a detergent contaminated pond situated in varanasi city india, which was ab ... | 2013 | 25320412 |
| metabolism dependent chemotaxis of pseudomonas aeruginosa n1 towards anionic detergent sodium dodecyl sulfate. | sodium dodecyl sulfate (sds) is one of the most commonly used detergent, which exhibits excellent biocidal activity against various bacteria and fungi. it is commonly employed in many detergent formulations and is employed for disinfection purposes. it is shown to be toxic to fishes, aquatic animals and is also inhibitory to microbes and cyanobacteria. we had isolated a strain belonging to pseudomonas aeruginosa n1, from a detergent contaminated pond situated in varanasi city india, which was ab ... | 2013 | 25320412 |
| effects of binary mixtures of inducers (toluene analogs) and of metals on bioluminescence induction of a recombinant bioreporter strain. | this paper investigated the effects of binary mixtures of bioluminescence inducers (toluene, xylene isomers, m-toluate) and of metals (cu, cd, as(iii), as(v), and cr) on bioluminescence activity of recombinant (pm-lux) strain kg1206. different responses and sensitivities were observed depending on the types and concentrations of mixtures of inducers or metals. in the case of inducer mixtures, antagonistic and synergistic modes of action were observed, whereas metal mixtures showed all three mode ... | 2014 | 25313497 |
| high cell density cultivation of pseudomonas putida kt2440 using glucose without the need for oxygen enriched air supply. | high cell density (hcd) cultivation of bacteria is essential for the majority of industrial processes to achieve high volumetric productivity (g l(-1) h(-1) ) of a bioproduct of interest. this study developed a fed batch bioprocess using glucose as sole carbon and energy source for the hcd of the well described biocatalyst pseudomonas putida kt2440 without the supply of oxygen enriched air. growth kinetics data from batch fermentations were used for building a bioprocess model and designing feed ... | 2015 | 25311981 |
| bioremediation in marine ecosystems: a computational study combining ecological modeling and flux balance analysis. | the pressure to search effective bioremediation methodologies for contaminated ecosystems has led to the large-scale identification of microbial species and metabolic degradation pathways. however, minor attention has been paid to the study of bioremediation in marine food webs and to the definition of integrated strategies for reducing bioaccumulation in species. we propose a novel computational framework for analysing the multiscale effects of bioremediation at the ecosystem level, based on co ... | 2014 | 25309577 |
| molecular dynamics of the p450cam-pdx complex reveals complex stability and novel interface contacts. | cytochrome p450cam catalyzes the stereo and regiospecific hydroxylation of camphor to 5-exo-hydroxylcamphor. the two electrons for the oxidation of camphor are provided by putidaredoxin (pdx), a fe2 s2 containing protein. two recent crystal structures of the p450cam-pdx complex, one solved with the aid of covalent cross-linking and one without, have provided a structural picture of the redox partner interaction. to study the stability of the complex structure and the minor differences between th ... | 2014 | 25307478 |
| molecular dynamics of the p450cam-pdx complex reveals complex stability and novel interface contacts. | cytochrome p450cam catalyzes the stereo and regiospecific hydroxylation of camphor to 5-exo-hydroxylcamphor. the two electrons for the oxidation of camphor are provided by putidaredoxin (pdx), a fe2 s2 containing protein. two recent crystal structures of the p450cam-pdx complex, one solved with the aid of covalent cross-linking and one without, have provided a structural picture of the redox partner interaction. to study the stability of the complex structure and the minor differences between th ... | 2014 | 25307478 |
| metabolic engineering of arabidopsis for remediation of different polycyclic aromatic hydrocarbons using a hybrid bacterial dioxygenase complex. | the widespread presence of polycyclic aromatic hydrocarbons (pahs) and their potential harm to various organisms has generated interest in efficiently eliminating these compounds from the environment. phytoremediation is an efficient technology for cleaning up pollutants. however, unlike microorganisms, plants lack the catabolic pathway for complete degradation of these dangerous groups of compounds. one way to enhance the potential of plants for remediation of these compounds is by transferring ... | 2014 | 25305469 |
| coevolution of the atpase clpv, the sheath proteins tssb and tssc, and the accessory protein tagj/hsie1 distinguishes type vi secretion classes. | the type vi secretion system (t6ss) is a bacterial nanomachine for the transport of effector molecules into prokaryotic and eukaryotic cells. it involves the assembly of a tubular structure composed of tssb and tssc that is similar to the tail sheath of bacteriophages. the sheath contracts to provide the energy needed for effector delivery. the aaa(+) atpase clpv disassembles the contracted sheath, which resets the systems for reassembly of an extended sheath that is ready to fire again. this me ... | 2014 | 25305017 |
| recovery of plasmid pemb1, whose toxin-antitoxin system stabilizes an ampicillin resistance-conferring β-lactamase gene in escherichia coli, from natural environments. | non-culture-based procedures were used to investigate plasmids showing ampicillin resistance properties in two different environments: remote mountain soil (mt. jeombong) and sludge (tancheon wastewater treatment plant). total dna extracted from the environmental samples was directly transformed into escherichia coli top10, and a single and three different plasmids were obtained from the mountain soil and sludge samples, respectively. interestingly, the restriction fragment length polymorphism p ... | 2014 | 25304509 |
| recovery of plasmid pemb1, whose toxin-antitoxin system stabilizes an ampicillin resistance-conferring β-lactamase gene in escherichia coli, from natural environments. | non-culture-based procedures were used to investigate plasmids showing ampicillin resistance properties in two different environments: remote mountain soil (mt. jeombong) and sludge (tancheon wastewater treatment plant). total dna extracted from the environmental samples was directly transformed into escherichia coli top10, and a single and three different plasmids were obtained from the mountain soil and sludge samples, respectively. interestingly, the restriction fragment length polymorphism p ... | 2014 | 25304509 |
| genetic and phenotypic characterization of the heat shock response in pseudomonas putida. | molecular chaperones function in various important physiological processes. null mutants of genes for the molecular chaperone clpb (hsp104), and those that encode j-domain proteins (dnaj, cbpa, and djla), which may act as hsp40 co-chaperones of dnak (hsp70), were constructed from pseudomonas putida kt2442 (kt) to elucidate their roles. the ktδclpb mutant showed the same heat shock response (hsr) as the wild-type, both in terms of heat-shock protein (hsp) synthesis (other than clpb) and in hsp ge ... | 2014 | 25303383 |
| production of recombinant proteins in mycobacterium smegmatis for structural and functional studies. | protein production using recombinant dna technology has a fundamental impact on our understanding of biology through providing proteins for structural and functional studies. escherichia coli (e. coli) has been traditionally used as the default expression host to over-express and purify proteins from many different organisms. e. coli does, however, have known shortcomings for obtaining soluble, properly folded proteins suitable for downstream studies. these shortcomings are even more pronounced ... | 2014 | 25303009 |
| production of recombinant proteins in mycobacterium smegmatis for structural and functional studies. | protein production using recombinant dna technology has a fundamental impact on our understanding of biology through providing proteins for structural and functional studies. escherichia coli (e. coli) has been traditionally used as the default expression host to over-express and purify proteins from many different organisms. e. coli does, however, have known shortcomings for obtaining soluble, properly folded proteins suitable for downstream studies. these shortcomings are even more pronounced ... | 2014 | 25303009 |
| study on the promotion of bacterial biofilm formation by a salmonella conjugative plasmid and the underlying mechanism. | to investigate the effect of the prst98 plasmid, originally isolated from salmonella enterica serovar typhi (s. typhi), on biofilm (bf) formation, we carried out in vitro experiments using s. typhi, salmonella enterica serovar typhimurium (s. typhimurium) and escherichia coli (e. coli). we further explored the effects of prst98 in vivo by establishing two animal models, a tumor-bearing mouse model and a mouse urethral catheter model. moreover, we examined the relationship between the quorum-sens ... | 2014 | 25299072 |
| the beneficial fungus piriformospora indica protects arabidopsis from verticillium dahliae infection by downregulation plant defense responses. | verticillium dahliae (vd) is a soil-borne vascular pathogen which causes severe wilt symptoms in a wide range of plants. the microsclerotia produced by the pathogen survive in soil for more than 15 years. | 2014 | 25297988 |
| molecular annotation of ketol-acid reductoisomerases from streptomyces reveals a novel amino acid biosynthesis interlock mediated by enzyme promiscuity. | the 6-phosphogluconate dehydrogenase superfamily oxidize and reduce a wide range of substrates, making their functional annotation challenging. ketol-acid reductoisomerase (kari), encoded by the ilvc gene in branched-chain amino acids biosynthesis, is a promiscuous reductase enzyme within this superfamily. here, we obtain steady-state enzyme kinetic parameters for 10 ilvc homologues from the genera streptomyces and corynebacterium, upon eight selected chemically diverse substrates, including som ... | 2014 | 25296650 |
| molecular annotation of ketol-acid reductoisomerases from streptomyces reveals a novel amino acid biosynthesis interlock mediated by enzyme promiscuity. | the 6-phosphogluconate dehydrogenase superfamily oxidize and reduce a wide range of substrates, making their functional annotation challenging. ketol-acid reductoisomerase (kari), encoded by the ilvc gene in branched-chain amino acids biosynthesis, is a promiscuous reductase enzyme within this superfamily. here, we obtain steady-state enzyme kinetic parameters for 10 ilvc homologues from the genera streptomyces and corynebacterium, upon eight selected chemically diverse substrates, including som ... | 2014 | 25296650 |
| cyp105-diverse structures, functions and roles in an intriguing family of enzymes in streptomyces. | the cytochromes p450 (cyp or p450) are a large superfamily of haem-containing enzymes found in all domains of life. they catalyse a variety of complex reactions, predominantly mixed-function oxidations, often displaying highly regio- and/or stereospecific chemistry. in streptomycetes, they are predominantly associated with secondary metabolite biosynthetic pathways or with xenobiotic catabolism. homologues of one family, cyp105, have been found in all streptomyces species thus far sequenced. thi ... | 2014 | 25294646 |
| non-natural olefin cyclopropanation catalyzed by diverse cytochrome p450s and other hemoproteins. | recent work has shown that engineered variants of cytochrome p450bm3 (cyp102a1) efficiently catalyze non-natural reactions, including carbene and nitrene transfer reactions. given the broad substrate range of natural p450 enzymes, we set out to explore if this diversity could be leveraged to generate a broad panel of new catalysts for olefin cyclopropanation (i.e., carbene transfer). here, we took a step towards this goal by characterizing the carbene transfer activities of four new wild-type p4 ... | 2014 | 25294253 |
| cytosine chemoreceptor mcpc in pseudomonas putida f1 also detects nicotinic acid. | soil bacteria are generally capable of growth on a wide range of organic chemicals, and pseudomonads are particularly adept at utilizing aromatic compounds. pseudomonads are motile bacteria that are capable of sensing a wide range of chemicals, using both energy taxis and chemotaxis. whilst the identification of specific chemicals detected by the ≥26 chemoreceptors encoded in pseudomonas genomes is ongoing, the functions of only a limited number of pseudomonas chemoreceptors have been revealed t ... | 2014 | 25294107 |
| modulation of quorum sensing in acylhomoserine lactone-producing or -degrading tobacco plants leads to alteration of induced systemic resistance elicited by the rhizobacterium serratia marcescens 90-166. | numerous root-associated bacteria (rhizobacteria) are known to elicit induced systemic resistance (isr) in plants. bacterial cell-density-dependent quorum sensing (qs) is thought to be important for isr. here, we investigated the role of qs in the isr elicited by the rhizobacterium, serratia marcescens strain 90-166, in tobacco. since s. marcescens 90-166 produces at least three qs signals, qs-mediated isr in strain 90-166 has been difficult to understand. therefore, we investigated the isr capa ... | 2013 | 25288945 |
| biocontrol of phytophthora blight and anthracnose in pepper by sequentially selected antagonistic rhizobacteria against phytophthora capsici. | we previously developed a sequential screening procedure to select antagonistic bacterial strains against phytophthora capsici in pepper plants. in this study, we used a modified screening procedure to select effective biocontrol strains against p. capsici; we evaluated the effect of selected strains on phytophthora blight and anthracnose occurrence and fruit yield in pepper plants under field and plastic house conditions from 2007 to 2009. we selected four potential biocontrol strains (pseudomo ... | 2013 | 25288942 |
| catechol biosynthesis from glucose in escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from pseudomonas aeruginosa pao1. | the aromatic compound catechol is used as a precursor of chemical products having multiple applications. this compound is currently manufactured by chemical synthesis from petroleum-derived raw materials. the capacity to produce catechol is naturally present in several microbial species. this knowledge has been applied to the generation of recombinant escherichia coli strains that can produce catechol from simple carbon sources. | 2014 | 25281236 |
| identification of multidrug-resistant bacteria and bacillus cereus from healthcare workers and environmental surfaces in a hospital. | 2014 | 25280977 | |
| coordinated response of phospholipids and acyl components of membrane lipids in pseudomonas putida a (atcc 12633) under stress caused by cationic surfactants. | the present study assessed the role of membrane components of pseudomonas putida a (atcc 12633) under chemical stress conditions originated by treatment with tetradecyltrimethylammonium bromide (ttab), a cationic surfactant. we examined changes in fatty acid composition and in the fluidity of the membranes of cells exposed to ttab at a specific point of growth as well as of cells growing with ttab. the addition of 10-50 mg ttab l(-1) promoted an increase in the saturated/unsaturated fatty acid r ... | 2014 | 25280753 |
| how to find rna thermometers. | temperature is one of the decisive signals that a mammalian pathogen has entered its warm-blooded host. among the many ways to register temperature changes, bacteria often use temperature-modulated structures in the untranslated region of mrnas. in this article, we describe how such rna thermometers (rnats) have been discovered one by one upstream of heat shock and virulence genes in the past, and how next-generation sequencing approaches are able to reveal novel temperature-responsive rna struc ... | 2014 | 25279353 |
| molecular diversity and functional variability of environmental isolates of bacillus species. | in the present study, out of 264 phosphate (p) solubilizing bacillus strains isolated from apple rhizosphere, only twelve isolates were found to be efficient (showed most of the plant growth promoting activity) which were further characterized at molecular level using 16s rdna partial gene sequencing. out of 12 isolates, mzpsb 207 was found to be most efficient p-solubilizing (864.71 μg/ml) isolate which also showed indole acetic acid production (51.83 μg/ml), siderophore production, ammonia pro ... | 2014 | 25279279 |
| degradation, phytoprotection and phytoremediation of phenanthrene by endophyte pseudomonas putida, pd1. | endophytes have been isolated from a large diversity of plants and have been shown to enhance the remediation efficiency of plants, but little information is available on the influence of endophytic bacteria on phytoremediation of widespread environmental contaminants such as polycyclic aromatic hydrocarbons (pahs). in this study we selected a naturally occurring endophyte for its combined ability to colonize plant roots and degrade phenanthrene in vitro. inoculation of two different willow clon ... | 2014 | 25275224 |
| metagenome-wide association of microbial determinants of host phenotype in drosophila melanogaster. | animal-associated bacteria (microbiota) affect host behaviors and physiological traits. to identify bacterial genetic determinants of microbiota-responsive host traits, we employed a metagenome-wide association (mgwa) approach in two steps. first, we measured two microbiota-responsive host traits, development time and triglyceride (tag) content, in drosophila melanogaster flies monoassociated with each of 41 bacterial strains. the effects of monoassociation on host traits were not confined to pa ... | 2014 | 25271286 |
| gibberellin secreting rhizobacterium, pseudomonas putida h-2-3 modulates the hormonal and stress physiology of soybean to improve the plant growth under saline and drought conditions. | the physiological changes in tolerant soybean plants under salt and drought stress conditions with pseudomonas putida h-2-3 were investigated. a bacterial isolate h-2-3 was isolated from soil and identified as pseudomonas putida h-2-3 by 16s rdna sequences. the treatment of p. putida h-2-3 significantly increased the length, fresh and dry weight of shoot and chlorophyll content in gibberellins (gas) deficient mutant waito-c rice seedlings over the control, it might be the presence of ga1, ga4, g ... | 2014 | 25270162 |
| microscopical observation of inhibition-behaviors against diaporthe citri by pre-treated with pseudomonas putida strain thj609-3 on the leaves of citrus plants. | citrus melanose is one of the most important diseases in orchards cultivating citrus in the world. although the disease does not cause yield loss, the profitability of the infected fruits is often reduced in the fresh-market, resulting in economic loss. in this study, disease reduction was proven by pre-treatment with pseudomonas putida strain thj609-3. in order to illustrate mechanism of the disease reduction by the bacterial strain, the infection behaviors of diaporthe citri and necrosis depos ... | 2014 | 25269607 |
| an aerobic exercise: defining the roles of pseudomonas aeruginosa terminal oxidases. | the opportunistic pathogen pseudomonas aeruginosa encodes a large and diverse complement of aerobic terminal oxidases, which is thought to contribute to its ability to thrive in settings with low oxygen availability. in this issue, arai et al. (j. bacteriol. 196:4206-4215, 2014, doi:http://dx.doi.org/10.1128/jb.02176-14) present a thorough characterization of these five complexes, enabling a more detailed understanding of aerobic respiration in this organism. | 2014 | 25266389 |
| synthetic vanillate-regulated promoter for graded gene expression in sphingomonas. | regulated promoters are an important basic genetic tool allowing, for example, gene-dosage and gene depletion studies. we have previously described a cumate-inducible promoter (p(q5)) that is functional in diverse alphaproteobacteria. this promoter has been engineered by combining a synthetic minimal promoter, p(syn2), and operator sites and the repressor of the pseudomonas putida f1 cym/cmt system. in the present study, we engineered a vanillate-regulated promoter using p(syn2) and the regulato ... | 2014 | 25262659 |
| silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent pseudomonas putida in laboratory medium and artificial wastewater. | bacteria based ecotoxicology assessment of manufactured nanoparticles is largely restricted to escherichia coli bioreporters in laboratory media. here, toxicity effects of model oecd nanoparticles (ag nm-300k, zno nm-110 and tio2 nm-104) were assessed using the switch-off luminescent pseudomonas putida bs566::luxcdabe bioreporter in luria bertani (lb) medium and artificial wastewater (aw). ic50 values ∼4 mg l(-1), 100 mg l(-1) and >200 mg l(-1) at 1 h were observed in lb for ag nm-300k, zno nm-1 ... | 2014 | 25261625 |
| anaerobic activation of p-cymene in denitrifying betaproteobacteria: methyl group hydroxylation versus addition to fumarate. | the betaproteobacteria "aromatoleum aromaticum" pcyn1 and "thauera" sp. strain pcyn2 anaerobically degrade the plant-derived aromatic hydrocarbon p-cymene (4-isopropyltoluene) under nitrate-reducing conditions. metabolite analysis of p-cymene-adapted "a. aromaticum" pcyn1 cells demonstrated the specific formation of 4-isopropylbenzyl alcohol and 4-isopropylbenzaldehyde, whereas with "thauera" sp. pcyn2, exclusively 4-isopropylbenzylsuccinate and tentatively identified (4-isopropylphenyl)itaconat ... | 2014 | 25261521 |
| fis overexpression enhances pseudomonas putida biofilm formation by regulating the ratio of lapa and lapf. | bacteria form biofilm as a response to a number of environmental signals that are mediated by global transcription regulators and alarmones. here we report the involvement of the global transcription regulator fis in pseudomonas putida biofilm formation through regulation of lapa and lapf genes. the major component of p. putida biofilm is proteinaceous and two large adhesive proteins, lapa and lapf, are known to play a key role in its formation. we have previously shown that fis overexpression e ... | 2014 | 25253613 |
| l-methionase: a therapeutic enzyme to treat malignancies. | cancer is an increasing cause of mortality and morbidity throughout the world. l-methionase has potential application against many types of cancers. l-methionase is an intracellular enzyme in bacterial species, an extracellular enzyme in fungi, and absent in mammals. l-methionase producing bacterial strain(s) can be isolated by 5,5'-dithio-bis-(2-nitrobenzoic acid) as a screening dye. l-methionine plays an important role in tumour cells. these cells become methionine dependent and eventually fol ... | 2014 | 25250324 |
| electricity generation and wastewater treatment of oil refinery in microbial fuel cells using pseudomonas putida. | microbial fuel cells (mfcs) represent a novel platform for treating wastewater and at the same time generating electricity. using pseudomonas putida (bcrc 1059), a wild-type bacterium, we demonstrated that the refinery wastewater could be treated and also generate electric current in an air-cathode chamber over four-batch cycles for 63 cumulative days. our study indicated that the oil refinery wastewater containing 2213 mg/l (ppm) chemical oxygen demand (cod) could be used as a substrate for ele ... | 2014 | 25247576 |
| colonization on root surface by a phenanthrene-degrading endophytic bacterium and its application for reducing plant phenanthrene contamination. | a phenanthrene-degrading endophytic bacterium, pn2, was isolated from alopecurus aequalis sobol grown in soils contaminated with polycyclic aromatic hydrocarbons (pahs). based on morphology, physiological characteristics and the 16s rrna gene sequence, it was identified as massilia sp. strain pn2 could degrade more than 95% of the phenanthrene (150 mg · l(-1)) in a minimal salts medium (msm) within 48 hours at an initial ph of 7.0 and a temperature of 30 °c. pn2 could grow well on the msm plates ... | 2014 | 25247301 |
| differential effects of a mutation on the normal and promiscuous activities of orthologs: implications for natural and directed evolution. | neutral drift occurring over millions or billions of years results in substantial sequence divergence among enzymes that catalyze the same reaction. although natural selection maintains the primary activity of orthologous enzymes, there is, by definition, no selective pressure to maintain physiologically irrelevant promiscuous activities. thus, the levels and the evolvabilities of promiscuous activities may vary among orthologous enzymes. consistent with this expectation, we have found that the ... | 2014 | 25246702 |
| differential effects of a mutation on the normal and promiscuous activities of orthologs: implications for natural and directed evolution. | neutral drift occurring over millions or billions of years results in substantial sequence divergence among enzymes that catalyze the same reaction. although natural selection maintains the primary activity of orthologous enzymes, there is, by definition, no selective pressure to maintain physiologically irrelevant promiscuous activities. thus, the levels and the evolvabilities of promiscuous activities may vary among orthologous enzymes. consistent with this expectation, we have found that the ... | 2014 | 25246702 |
| shigella ipah7.8 e3 ubiquitin ligase targets glomulin and activates inflammasomes to demolish macrophages. | when nucleotide-binding oligomerization domain-like receptors (nlrs) sense cytosolic-invading bacteria, they induce the formation of inflammasomes and initiate an innate immune response. in quiescent cells, inflammasome activity is tightly regulated to prevent excess inflammation and cell death. many bacterial pathogens provoke inflammasome activity and induce inflammatory responses, including cell death, by delivering type iii secreted effectors, the rod component flagellin, and toxins. recent ... | 2014 | 25246571 |
| bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of leclercia sp. qau-66. | glucose dehydrogenase (gdh; ec 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. in present study, leclercia sp. qau-66, isolated from rhizosphere of vigna mungo, was characterized for phosphate solubilization and the role of gdh in plant growth promotion of phaseolus vulgaris. the strain qau-66 had ability to solubilize phosphorus and significantly (p ≤ 0.05) promoted the shoot and root l ... | 2014 | 25242947 |
| growth kinetics, effect of carbon substrate in biosynthesis of mcl-pha by pseudomonas putida bet001. | growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-pha) in pseudomonas putida bet001 isolated from palm oil mill effluent was studied. models with substrate inhibition terms described well the kinetics of its growth. selected fatty acids (c8:0 to c18:1) and ammonium were used as carbon and nitrogen sources during growth and pha biosynthesis, resulting in pha accumulation of about 50 to 69% (w/w) and pha yields ranging from 10.12 g l(-1) to 15.45 g l(-1), respecti ... | 2014 | 25242925 |
| cloning and characterization of a novel esterase from rhodococcus sp. for highly enantioselective synthesis of a chiral cilastatin precursor. | a novel nonheme chloroperoxidase (rhest1), with promiscuous esterase activity for enantioselective hydrolysis of ethyl (s)-2,2-dimethylcyclopropanecarboxylate, was identified from a shotgun library of rhodococcus sp. strain ecu1013. rhest1 was overexpressed in escherichia coli bl21(de3), purified to homogeneity, and functionally characterized. fingerprinting analysis revealed that rhest1 prefers para-nitrophenyl (pnp) esters of short-chain acyl groups. pnp esters with a cyclic acyl moiety, espec ... | 2014 | 25239898 |
| conversion of the pseudomonas aeruginosa quinolone signal and related alkylhydroxyquinolines by rhodococcus sp. strain bg43. | a bacterial strain, which based on the sequences of its 16s rrna, gyrb, cata, and qsda genes, was identified as a rhodococcus sp. closely related to rhodococcus erythropolis, was isolated from soil by enrichment on the pseudomonas quinolone signal [pqs; 2-heptyl-3-hydroxy-4(1h)-quinolone], a quorum sensing signal employed by the opportunistic pathogen pseudomonas aeruginosa. the isolate, termed rhodococcus sp. strain bg43, cometabolically degraded pqs and its biosynthetic precursor 2-heptyl-4(1h ... | 2014 | 25239889 |
| clar--a novel key regulator of cellobiose and lactose metabolism in lactococcus lactis il1403. | in a number of previous studies, our group has discovered an alternative pathway for lactose utilization in lactococcus lactis that, in addition to a sugar-hydrolyzing enzyme with both p-β-glucosidase and p-β-galactosidase activity (bgls), engages chromosomally encoded components of cellobiose-specific pts (pts(cel-lac)), including ptca, ptcb, and celb. in this report, we show that this system undergoes regulation via clar, a novel activator protein from the rpir family of transcriptional regula ... | 2014 | 25239037 |
| clar--a novel key regulator of cellobiose and lactose metabolism in lactococcus lactis il1403. | in a number of previous studies, our group has discovered an alternative pathway for lactose utilization in lactococcus lactis that, in addition to a sugar-hydrolyzing enzyme with both p-β-glucosidase and p-β-galactosidase activity (bgls), engages chromosomally encoded components of cellobiose-specific pts (pts(cel-lac)), including ptca, ptcb, and celb. in this report, we show that this system undergoes regulation via clar, a novel activator protein from the rpir family of transcriptional regula ... | 2014 | 25239037 |
| selective methioninase-induced trap of cancer cells in s/g2 phase visualized by fucci imaging confers chemosensitivity. | a major impediment to the response of tumors to chemotherapy is that the large majority of cancer cells within a tumor are quiescent in g0/g1, where cancer cells are resistant to chemotherapy. to attempt to solve this problem of quiescent cells in a tumor, cancer cells were treated with recombinant methioninase (rmetase), which selectively traps cancer cells in s/g2. the cell cycle phase of the cancer cells was visualized with the fluorescence ubiquitination-based cell cycle indicator cell cycle ... | 2014 | 25238266 |
| mining the metabiome: identifying novel natural products from microbial communities. | microbial-derived natural products provide the foundation for most of the chemotherapeutic arsenal available to contemporary medicine. in the face of a dwindling pipeline of new lead structures identified by traditional culturing techniques and an increasing need for new therapeutics, surveys of microbial biosynthetic diversity across environmental metabiomes have revealed enormous reservoirs of as yet untapped natural products chemistry. in this review, we touch on the historical context of mic ... | 2014 | 25237864 |
| synthetic biology and metabolic engineering for marine carotenoids: new opportunities and future prospects. | carotenoids are a class of diverse pigments with important biological roles such as light capture and antioxidative activities. many novel carotenoids have been isolated from marine organisms to date and have shown various utilizations as nutraceuticals and pharmaceuticals. in this review, we summarize the pathways and enzymes of carotenoid synthesis and discuss various modifications of marine carotenoids. the advances in metabolic engineering and synthetic biology for carotenoid production are ... | 2014 | 25233369 |
| bacterial sigma factors as targets for engineered or synthetic transcriptional control. | sigma (σ) factors are the predominant constituents of transcription regulation in bacteria. σ factors recruit the core rna polymerase to recognize promoters with specific dna sequences. recently, engineering of transcriptional regulators has become a significant tool for strain engineering. the present review summarizes the recent advances in σ factor based engineering or synthetic design. the manipulation of σ factors presents insights into the bacterial stress tolerance and metabolite producti ... | 2014 | 25232540 |
| strain-specific bioaccumulation and intracellular distribution of cd²⁺ in bacteria isolated from the rhizosphere, ectomycorrhizae, and fruitbodies of ectomycorrhizal fungi. | bioaccumulation of cd(2+) in soil bacteria might represent an important route of metal transfer to associated mycorrhizal fungi and plants and may have potential as a tool to accelerate cd(2+) extraction in the bioremediation of contaminated soils. the present study examined the bioaccumulation of cd(2+) in 15 bacterial strains representing three phyla (firmicutes, proteobacteria, and bacteroidetes) that were isolated from the rhizosphere, ectomycorrhizae, and fruitbody of ectomycorrhizal fungi. ... | 2014 | 25231735 |
| strain-specific bioaccumulation and intracellular distribution of cd²⁺ in bacteria isolated from the rhizosphere, ectomycorrhizae, and fruitbodies of ectomycorrhizal fungi. | bioaccumulation of cd(2+) in soil bacteria might represent an important route of metal transfer to associated mycorrhizal fungi and plants and may have potential as a tool to accelerate cd(2+) extraction in the bioremediation of contaminated soils. the present study examined the bioaccumulation of cd(2+) in 15 bacterial strains representing three phyla (firmicutes, proteobacteria, and bacteroidetes) that were isolated from the rhizosphere, ectomycorrhizae, and fruitbody of ectomycorrhizal fungi. ... | 2014 | 25231735 |
| effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase. | pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenases (pqq-adh) require ammonia or primary amines as activators in in vitro assays with artificial electron acceptors. we found that pqq-adh from pseudomonas putida kt2440 (ppadh) was activated by various primary amines, di-methylamine, and tri-methylamine. the alcohol oxidation activity of ppadh was strongly enhanced and the affinity for substrates was also improved by pentylamine as an activator. | 2014 | 25229857 |
| photochemistry of flavoprotein light sensors. | three major classes of flavin photosensors, light oxygen voltage (lov) domains, blue light sensor using fad (bluf) proteins and cryptochromes (crys), regulate diverse biological activities in response to blue light. recent studies of structure, spectroscopy and chemical mechanism have provided unprecedented insight into how each family operates at the molecular level. in general, the photoexcitation of the flavin cofactor leads to changes in redox and protonation states that ultimately remodel p ... | 2014 | 25229449 |
| improved cytotoxic effects of salmonella-producing cytosine deaminase in tumour cells. | in order to increase the cytotoxic activity of a salmonella strain carrying a salicylate-inducible expression system that controls cytosine deaminase production, we have modified both, the vector and the producer bacterium. first, the translation rates of the expression module containing the escherichia coli coda gene cloned under the control of the pm promoter have been improved by using the t7 phage gene 10 ribosome binding site sequence and replacing the original gug start codon by aug. secon ... | 2014 | 25227763 |
| improved cytotoxic effects of salmonella-producing cytosine deaminase in tumour cells. | in order to increase the cytotoxic activity of a salmonella strain carrying a salicylate-inducible expression system that controls cytosine deaminase production, we have modified both, the vector and the producer bacterium. first, the translation rates of the expression module containing the escherichia coli coda gene cloned under the control of the pm promoter have been improved by using the t7 phage gene 10 ribosome binding site sequence and replacing the original gug start codon by aug. secon ... | 2014 | 25227763 |
| phytochemicals increase the antibacterial activity of antibiotics by acting on a drug efflux pump. | drug efflux pumps confer resistance upon bacteria to a wide range of antibiotics from various classes. the expression of efflux pumps are also implicated in virulence and biofilm formation. moreover, organisms can only acquire resistance in the presence of active drug efflux pumps. therefore, efflux pump inhibitors (epis) are attractive compounds to reverse multidrug resistance and to prevent the development of resistance in clinically relevant bacterial pathogens. we investigated the potential ... | 2014 | 25224951 |
| o serotype-independent susceptibility of pseudomonas aeruginosa to lectin-like pyocins. | lectin-like bacteriocins of the llpa family, originally identified in plant-associated bacteria, are narrow-spectrum antibacterial proteins composed of two tandemly organized monocot mannose-binding lectin (mmbl) domains. the llpa-like bacteriocin of pseudomonas aeruginosa c1433, pyocin l1, lacks any similarity to known p. aeruginosa bacteriocins. the initial interaction of pyocin l1 with target cells is mediated by binding to d-rhamnose, present in the common polysaccharide antigen of lipopolys ... | 2014 | 25224846 |
| bacterial population succession and adaptation affected by insecticide application and soil spraying history. | although microbial communities have varying degrees of exposure to environmental stresses such as chemical pollution, little is known on how these communities respond to environmental disturbances and how past disturbance history affects these community-level responses. to comprehensively understand the effect of organophosphorus insecticide application on microbiota in soils with or without insecticide-spraying history, we investigated the microbial succession in response to the addition of fen ... | 2014 | 25221549 |
| biocontrol and plant growth-promoting activity of rhizobacteria from chinese fields with contaminated soils. | the aim of this study was to inventory the types of plant growth-promoting rhizobacteria (pgpr) present in the rhizosphere of plants grown in soils contaminated with heavy metals, recalcitrant organics, petroleum sewage or salinity in china. we screened 1223 isolates for antifungal activity and about 24% inhibited rhizoctonia solani or sclerotinia sclerotiorum. twenty-four strains inhibitory to r. solani, gaeumannomyces graminis var. tritici and/or s. sclerotiorum and representing the dominant m ... | 2014 | 25219642 |
| biocontrol and plant growth-promoting activity of rhizobacteria from chinese fields with contaminated soils. | the aim of this study was to inventory the types of plant growth-promoting rhizobacteria (pgpr) present in the rhizosphere of plants grown in soils contaminated with heavy metals, recalcitrant organics, petroleum sewage or salinity in china. we screened 1223 isolates for antifungal activity and about 24% inhibited rhizoctonia solani or sclerotinia sclerotiorum. twenty-four strains inhibitory to r. solani, gaeumannomyces graminis var. tritici and/or s. sclerotiorum and representing the dominant m ... | 2014 | 25219642 |
| physical lysis only (plo) methods suitable as rapid sample pretreatment for qpcr assay. | quantitative pcr (qpcr) enables rapid and sensitive gene quantification and is widely used in genomics, such as biological, medical, environmental, and food sciences. however, sample pretreatment requires the use of conventional dna extraction kits which are time-consuming and labor intensive. in this study, we investigated four physical lysis only (plo) methods which are rapid and could serve as alternatives to conventional dna extraction kits. these plo methods are bead mill, heating, sonicati ... | 2014 | 25219535 |
| responses of unsaturated pseudomonas putida cz1 biofilms to environmental stresses in relation to the eps composition and surface morphology. | the extracellular polymeric substance (eps) and surface properties of unsaturated biofilms of a heavy metal-resistant rhizobacterium pseudomonas putida cz1, in response to aging, ph, temperature and osmotic stress, were studied by quantitative analysis of eps and atomic force microscope. it was found that eps production increased approximately linearly with culture time, cells in the air-biofilm interface enhanced eps production and decreased cell volume to cope with nutrient depletion during ag ... | 2014 | 25217027 |
| three-component o-demethylase system essential for catabolism of a lignin-derived biphenyl compound in sphingobium sp. strain syk-6. | sphingobium sp. strain syk-6 is able to assimilate lignin-derived biaryls, including a biphenyl compound, 5,5'-dehydrodivanillate (ddva). previously, ligxa (slg_07770), which is similar to the gene encoding oxygenase components of rieske-type nonheme iron aromatic-ring-hydroxylating oxygenases, was identified to be essential for the conversion of ddva; however, the genes encoding electron transfer components remained unknown. disruption of putative electron transfer component genes scattered thr ... | 2014 | 25217011 |
| cluster and sporadic cases of herbaspirillum species infections in patients with cancer. | herbaspirillum species are gram-negative betaproteobacteria that inhabit the rhizosphere. we investigated a potential cluster of hospital-based herbaspirillum species infections. | 2014 | 25216687 |
| cluster and sporadic cases of herbaspirillum species infections in patients with cancer. | herbaspirillum species are gram-negative betaproteobacteria that inhabit the rhizosphere. we investigated a potential cluster of hospital-based herbaspirillum species infections. | 2014 | 25216687 |
| the biosurfactant viscosin transiently stimulates n-hexadecane mineralization by a bacterial consortium. | pseudomonas produces powerful lipopeptide biosurfactants including viscosin, massetolide a, putisolvin, and amphisin, but their ability to stimulate alkane mineralization and their utility for bioremediation have received limited attention. the four pseudomonas lipopeptides yielded emulsification indices on hexadecane of 20-31% at 90 mg/l, which is comparable to values for the synthetic surfactant tween 80. viscosin was the optimal emulsifier and significantly stimulated n-hexadecane mineralizat ... | 2014 | 25216581 |
| the biosurfactant viscosin transiently stimulates n-hexadecane mineralization by a bacterial consortium. | pseudomonas produces powerful lipopeptide biosurfactants including viscosin, massetolide a, putisolvin, and amphisin, but their ability to stimulate alkane mineralization and their utility for bioremediation have received limited attention. the four pseudomonas lipopeptides yielded emulsification indices on hexadecane of 20-31% at 90 mg/l, which is comparable to values for the synthetic surfactant tween 80. viscosin was the optimal emulsifier and significantly stimulated n-hexadecane mineralizat ... | 2014 | 25216581 |
| differences between the rhizosphere microbiome of beta vulgaris ssp. maritima-ancestor of all beet crops-and modern sugar beets. | the structure and function of the plant microbiome is driven by plant species and prevailing environmental conditions. effectuated by breeding efforts, modern crops diverge genetically and phenotypically from their wild relatives but little is known about consequences for the associated microbiota. therefore, we studied bacterial rhizosphere communities associated with the wild beet b. vulgaris ssp. maritima grown in their natural habitat soil from coastal drift lines (cs) and modern sugar beets ... | 2014 | 25206350 |