Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| induction of alkane hydroxylase proteins by unoxidized alkane in pseudomonas putida. | in vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity. | 1975 | 1150630 |
| regulation of alkane oxidation in pseudomonas putida. | we have studied the appearance of whole-cell oxidizing activity for n-alkanes and their oxidation products in strains of pseudomonas putida carrying the oct plasmid. our results indicate that the oct plasmid codes for inducible alkane-hydroxylating and primary alcohol-dehydrogenating activities and that the chromosome codes for constitutive oxidizing activities for primary alcohols, aliphatic aldehydes, and fatty acids. mutant isolation confirms the presence of an alcohol dehydrogenase locus on ... | 1975 | 1150626 |
| the uptake of glucose and gluconate by pseudomonas putida. | the uptake of glucose and gluconate is under inductive control in pseudomonas putida. glucose, gluconate, and 2-ketogluconate were each good nutritional inducers of these transport abilities. glucose and gluconate uptake obeyed saturation kinetics: the apparent km for glucose was 6 mm and that for gluconate was 0.5 mm. therefore, transport of both substrates appears to be mediated by enzyme-like carriers. glucose and gluconate are parallel inhibitors for their uptake9 strains selected for their ... | 1975 | 1134500 |
| metabolism of resorcinylic compounds by bacteria. purification and properties of orcinol hydroxylase from pseudomonas putida 01. | orcinol hydroxylase (ec 1.14.13.6), which catalyzes the first reaction of orcinol catabolism in pseudomonas putida 01, has been purified to homogeneity, and crystallized. orcinol hydroxylase catalyzes the hydroxylation of orcinol with equimolar consumption of o2 and nadh (or nadph) to 2, 3, 5-trihydroxytoluene, which is nonenzymically oxidized to a quinone. the visible absorption spectrum of the enzyme shows maxima at 373 and 454 nm and a shoulder at 480 nm. fad can be dissociated from the prote ... | 1975 | 1126936 |
| pathways for the degradation of m-cresol and p-cresol by pseudomonas putida. | a comparison of the oxidation rates of various compounds by whole cells of pseudomonas putida 3, 5 indicated that m-cresol is metabolized by oxidation to 3-hydroxybenzoate followed by hydroxylation to gentisate, the ring-fission substrate, when grown with 3, 5-xylenol. however, when m-cresol was the growth substrate, similar experiments suggested a different pathway involving a methyl-substituted catechol, and ring-fission by meta cleavage. assays of ring-fission enzymes in cell-free extracts co ... | 1975 | 1123316 |
| the uptake of fructose by pseudomonas putida. | fructose transport was not apparently affected in a number of pseudomonas putida strains with deranged activity of a common glucose-gluconate uptake system, indicating the existence of an independent fructose uptake system. fructose uptake by glucose-gluconate uptake mutants was induced by fructose and obeyed saturation kinetics (apparent km equal 0.3 mm). the fructose uptake system serves to transport glucose in addition to fructose. the entry of fructose into p.putida cells appears to be media ... | 1975 | 1115560 |
| purification and characterization of bacteriophage gh-i-induced deoxyribonucleic acid-dependent ribonucleic acid polymerase from pseudomonas putida. | infection of pseudomonas putida by the bacteriophage gh-l-induced the synthesis of a novel dna-dependent rna polymerase. this gh-l-induced rna polymerase was purified to near homogeneity. it was shown to be distinct from the host rna polymerase (alpha-2 beta beta sigma) physically and in respect to many of its catalytic properties. the gh-l-induced rna polymerase was composed of a single polypeptide of approximately 98,000 molecular weight. the divalent metal ion requirement for in vitro rna syn ... | 1975 | 1112826 |
| improved method of selection for mutants of pseudomonas putida. | optimum conditions for enrichment of mutants of pseudomonas putida in liquid culture were established using a procedure which combines n-methyl-n'-nitro-n-nitrosoguanidine mutagenesis with an improved d-cycloserine selection. | 1975 | 1108792 |
| transformation of pseudomonas putida and escherichia coli with plasmid-linked drug-resistance factor dna. | conditions optimal for the transformation of pseudomonas putida and e. coli with a drug-resistance factor (rp 1) dna, which specifies resistance to carbenicillin, tetracycline, kanamycin, and neomycin, are described. the transformants retain all the fertility, incompatibility, and drug-resistance characteristics present in the parent. covalently-closed circular molecules of almost identical contour lengths have been isolated from the parent and the transformants. the frequency of transformation ... | 1975 | 1103151 |
| lipoate metabolism in pseudomonas putida lp. | 1975 | 1099990 | |
| characterization of the growth of pseudomonas putida lp on lipoate and its analogues: transport, oxidation, sulphur source, and enzyme induction. | pseudomonas putida lp, which grows on lipoate, nh4no3 and mineral salts, converts most of the organic substrate to bisnor-lipoate (1,2-dithiolane-3-propanoic acid) and acetyl-coa. d-, l-, or dl-lipoate serve equally well as carbon and sulphur sources. there was no growth on or bacterial oxidation of the chemically synthesized bisnor- or tetranor-(1,2-dithiolane-3-carboxylic acid) chain-shortened analogues, but these, as well as lipoate, could supply the sulphur needed for growth when acetate was ... | 1975 | 1089758 |
| autogenous regulation of the inducible tryptophan synthase of pseudomonas putida. | mutants blocked before indole-3-glycerol phosphate formation in the tryptophan biosynthetic pathway of p. putida ("early-blocked" mutants) are unable to use indole as a source of tryptophan for growth on minimal medium. the uninduced level of tryptophan synthase [ec 4.2.1.20; l-serine hydro-lyase (adding indole)] in such mutants was thought to be responsible for this property. we have shown that levels of indole higher than those previously tested will support growth of these mutants. in additio ... | 1975 | 1055401 |
| [generalized transduction of pseudomonas putida with a thermosensitive mutant of phage pf16h2]. | 1976 | 1032694 | |
| regulation of the meta-cleavage of 4-hydroxyphenylacetic acid by pseudomonas putida. | 1976 | 1027447 | |
| the uptake of 2-ketogluconate by pseudomonas putida. | the uptake of 2-ketogluconate is inducible in pseudomonas putida: 2-ketogluconate, glucose, gluconate, glycerol and glycerate were each good nutritional inducers of this ability. 2-ketogluconate uptake obeyed saturation kinetics (apparent km in 2-ketogluconate-grown cells was 0.4 mm). 2-ketogluconate was transported against a concentration gradient, apparently in an unchanged state, and the process required metabolic energy, all of which indicate an active transport system. a number of independe ... | 1976 | 1015939 |
| characterization of a benzoate permease mutant of pseudomonas putida. | a spontaneous mutant of pseudomonas putida (prs 2017) has been isolated which is incapable of growth on benzoate, does not induce the enzymes of the catechol branch of the beta-ketoadipate pathway when grown in the presence of benzoate, cannot accumulate radioactively labeled benzoate, yet grows well with mandelate as sole source of carbon and energy. this strain apparently lacks a benzoate permease, which in the wild type shows a km of about 0.1 mm for benzoate, is inducible, and is not under t ... | 1976 | 1015938 |
| the effect of a non-metabolizable analog on mandelate catabolism in pseudomonas putida. | dl-2,3,4,5,6-pentafluoromandelic acid (pfm) specifically inhibits the growth of pseudomonas putida (atcc 12633) on medium containing mandelate as sole carbon and energy source by competitive inhibition of mandelate dehydrogenase. pfm is not metabolized and is neither an inducer of the mandelate catabolic enzymes nor an antagonist of induction. mutants resistant to the inhibitory effects of pfm (pfmr) were isolated; most prove to be superinducible, i.e. synthesize corrdinately the mandelate-speci ... | 1976 | 1015936 |
| creatine amidinohydrolase of pseudomonas putida: crystallization and some properties. | 1976 | 1015832 | |
| combined chromosomal and plasmid encoded control for the degradation of phenol in pseudomonas putida. | 1976 | 1001897 | |
| aromatic-alcohol dehydrogenases from pseudomonas putida n.c.i.b. 9869. | 1976 | 1001711 | |
| [microbial breakdown of caffeine (author's transl)]. | a bacterium, capable of growing aerobically with caffeine as its sole source of carbon and nitrogen, was isolated from soil and identified as pseudomonas putida sp. the breakdown of caffeine begins with stepwise demethylation, which leads via various n-methyl-purines to xanthine each step yielding formaldehyde. xanthine is then broken down via uric acid, allantoin, allantoic acid and further intermediates to urea and glyoxylic acid, which serves as the actual source of carbon. | 1976 | 998047 |
| purification and properties of l-4-hydroxymandelate oxidase from pseudomonas convexa. | an inducible membrane-bound l-4-hydroxymandelate oxidase (decarboxylating) from pseudomonas convexa has been solubilized and partially purified. it catalyzes the conversion of l-4-hydroxymandelic acid to 4-hydroxybenzaldehyde in a single step with the stoichiometric consumption of o2 and liberation of co2. the enzyme is optimally active at ph 6.6 and at 55 degrees c. it requires fad and mn2+ for its activity. the membrane-bound enzyme is more stable than the solubilized and purified enzyme. afte ... | 1976 | 976259 |
| enzyme evolution in a microbial community growing on the herbicide dalapon. | a seven-membered microbial community capable of utilising the herbicide dalapon has been isolated by continuous-flow enrichment culture. the composition of this community has remained remarkably stable over thousands of hours in a dalapon-limited chemostat. during this period, however, one member of the community, pseudomonas putida, acquired the ability to grow on dalapon through the evolution of an extant dehalogenase. | 1976 | 972691 |
| current status of the sequence studies of the pseudomonas putida camphor hydroxylase system. | 1976 | 961531 | |
| involvement of 4-hydroxymandelic acid in the degradation of mandelic acid by pseudomonas convexa. | a microorganism capable of degrading dl-mandelic acid was isolated from sewage sediment of enrichment culture and was identified as pseudomonas convexa. it was found to metabolize mandelic acid by a new pathway involving 4-hydroxymandelic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzoic acid as aromatic intermediates. all the enzymes of the pathway were demonstrated in cell-free extracts. l-mandelate-4-hydroxylase, a soluble enzyme, requires tetrahydropteridine, nicot ... | 1976 | 956122 |
| catechol oxygenases of pseudomonas putida mutant strains. | investigation of a mutant strain of pseudomonas putida ncib 10015, strain psu-e1, showed that it had lost the ability to produce catechol 1,2-oxygenase after growth with catechol. additional mutants of both wild-type and mutant strains psu-e1 have been isolated that grow on catechol, but not on benzoate, yet still form a catechol 1,2-oxygenase when exposed to benzoate. these findings indicate that either there are separately induced catechol 1,2-oxygenase enzymes, or that there are two separate ... | 1976 | 956121 |
| purification and properties of methioninase from pseudomonas ovalis. | 1976 | 955094 | |
| [pseudomonas putida plasmid controlling the initial stages of naphthalene oxidation]. | 1976 | 949929 | |
| crystallization and preliminary crystal data of iron-containing superoxide dismutase from pseudomonas ovalis. | large single crystals of iron-containing superoxide dismutase from pseudomonas ovalis were prepared from 50% saturated ammonium sulfate solution, at ph 4.5, on gentle evaporation of the solvent at 4 degrees. the crystals were monoclinic, space group p2, with unit cell dimensions a = 81.9 a, b = 49.0 a, c = 61.0 a, and beta = 106 degrees. considerations of cell volume and protein molecular weight indicated 1 molecule of superoxide dismutase in the assymmetric unit, the smallest number reported s ... | 1976 | 947910 |
| metabolism of resorcinylic compounds by bacteria: alternative pathways for resorcinol catabolism in pseudomonas putida. | two strains of pseudomonas putida isolated by enrichment cultures with orcinol as the sole source of carbon were both found to grow with resorcinol. data are presented which show that one strain (orc) catabolizes resorcinol by a metabolic pathway, genetically and mechanistically distinct from the orcinol pathway, via hydroxyquinol and ortho oxygenative cleavage to give maleylacetate, but that the other strain (o1) yields mutants that utilize resorcinol. one mutant strain, designated o1oc, was sh ... | 1976 | 942589 |
| the p-cymene pathway in pseudomonas putida pl: isolation of a dihydrodiol accumulated by a mutant. | 1976 | 942434 | |
| failure of complex supplementation of minimal cultures to elicit a shift-up response in pseudomonas putida. | the addition of complex supplements (particularly amino acids) to cultures of pseudomonas putida growing on a good carbon source did not result in a substantial increase in the growth rate. amino acids entered the cells within 30 s of addition and reached significant internal pool concentrations. endogenous amino acid biosynthesis was quickly inhibited (about 75%), with a substantial sparing of the original carbon source. within 20 min of supplementation significant respiration of added amino ac ... | 1976 | 932677 |
| purification and partial amino acid sequence of the cyanogen bromide fragments of muconolactone isomerase from pseudomonas putida. | muconolactone isomerase is shown to be resistant to proteolytic cleavage by trypsin. cyanogen bromide cleavage at the methionine residues of the polypeptide is at least 95% complete. six cyanogen bromide fragments are separated on deae-cellulose. one fragment is shown by amino acid analysis and carboxyl-terminal analysis to be an incomplete cleavage product. the five remaining fragments represent the entire polypeptide and have been ordered with respect to the entire muconolactone isomerase sequ ... | 1977 | 901811 |
| molecular characterization of hydrocarbon degradative plasmids in pseudomonas putida. | 1977 | 901483 | |
| two modes of loss of the tol function from pseudomonas putida mt-2. | some of a set of independently arising tol- (non toluate-utilising) derivatives of pseudomonas putida mt-2 have lost the unique plasmid present in the parent strain. in others this plasmid has suffered a deletion of a specific region of about 27 md. | 1977 | 895716 |
| myo-inositol transport system in pseudomonas putida. | the kinetic features of the myo-inositol transport system in pseudomonas putida are reported. the system is sensitive to osmotic shock, is not operative in membrane vesicles, and does not involved substrate phosphorylation. line-weaver-burk plots indicate the presence of two different systems, whose kt are 5 micrometer and 0.43 mm and whose v max are 7.9 and 27 nml/mg per min, respectively. transport activity of glucose-grown cells is very low. myo-inositol-grown cells lose the high-affinity sys ... | 1977 | 893343 |
| molecular sizes and relationships of tol plasmids in pseudomonas. | plasmid deoxyribonucleic acid was isolated from thirteen pseudomonas strains judged on genetic criteria to carry plasmids coding for the degradation of toluene and m- and p-xylenes (tol plasmids). most strains carried a single species, but two strains carried two size classes, and cells of a third strain contained plasmids ranging in size from 25 x 10(6) to 202 x 10(6) daltons. some plasmids could be transformed into a pseudomonas putida strain to yield tol+ progeny. plasmids from 5 of the 13 st ... | 1977 | 863855 |
| distribution of xanthine oxidase and xanthine dehydrogenase specificity types among bacteria. | a diverse collection of xanthine-metabolizing bacteria was examined for xanthine-, 1-methylxanthine-, and 3-methylxanthine-oxidizing activity. both particulate and soluble fractions of extracts from aerobically grown gram-negative bacteria exhibited oxidation of all three substrates; however, when facultative gram-negative bacteria were grown anaerobically, low particulate and 3-methylxanthine activities were detected. gram-positive and obligately anaerobic bacteria showed no particulate activit ... | 1977 | 863854 |
| characterization of a spontaneously occurring mutant of the tol20 plasmid in pseudomonas putida mt20: possible regulatory implications. | pseudomonas putida mt20 carries a plasmid (tol20) that codes for the enzymes responsible for the catabolism of toluene, m- and p-xylene to benzoate, and m- and p-toluate, respectively, followed by meta cleavage of the aromatic ring. growth on 5 mm benzoate selects very strongly for (i) strains that have been cured of the plasmid and (ii) strains with an intermediate growth pattern (the b3 phenotype) that retain the ability to grow on toluene, m-xylene, and benzoate but are unable to grow on m-to ... | 1977 | 863853 |
| crystalline cis-benzene glycol dehydrogenase from pseudomonas putida. | 1977 | 859182 | |
| mandelate racemase from pseudomonas putida. absence of detectable intermolecular proton transfer accompanying racemization. | an equimolar mixture of dl-[alpha-2h]- and dl-[alpha-13c]mandelate, when incubated with mandelate racemase (ec 5.1.2.2), shows conversion of singly labeled mandelate to unlabeled mandelate, due to solvent exchange of the alpha proton, while the level of doubly labeled mandelate remains at a constant low level. similarly, an equimolar mixture of unlabeled and dl-[alpha-2h,alpha-13c]mandelate, when incubated with the enzyme, shows conversion of doubly labeled mandelate to singly labeled mandelate, ... | 1977 | 849410 |
| isolation of metabolic plasmid dna from pseudomonas putida. | 1977 | 849300 | |
| p-cymene pathway in pseudomonas putida: ring cleavage of 2,3-dihydroxy-p-cumate and subsequent reactions. | it was confirmed that 2,3-dihydroxy-p-cumate is a substrate for ring cleavage in pseudomonas putida pl-w after growth with p-cymene or p-cumate. this compound was oxidized to pyruvate, acetaldehyde, isobutyrate, and carbon dioxide by extracts of cells, and these products appear in equimolar amounts. the transient appearance of compounds and 2,3-dihydroxy-p-cumate to a yellow intermediate (lambda max, 345 nm) without decarboxylation. extracts of the benzene nucleus; this is followed by decarboxyl ... | 1977 | 845118 |
| p-cymene pathway in pseudomonas putida: initial reactions. | initial reactions of the p-cymene pathway induced in pseudomonas putida pl have been reinvestigated. oxidation of the methyl group attached to the nucleus occurs in three steps to give p-cumic acid. the substrate for the ring cleavage of 2,3-dihydroxy-p-cumate is formed from p-cumate in two reactions via a dihydrodiol intermediate (2,3-dihydroxy-4-isopropylcyclohexa-4,6-dienoate) and not as previously postulated via 3-hydroxy-p-cumate. there are three pieces of evidence for the physiological rol ... | 1977 | 845117 |
| purification and properties of homoprotocatechuate 2,3-dioxygenase from bacillus stearothermophilus. | the enzyme 3,4-dihydroxyphenylacetate:oxygen 2,3-oxidoreductase (decyclizing) (homoprotocatechuate 2,3-dioxygenase) was purified from the thermophilic organism bacillus stearothermophilus, grown with j-hydroxyphenylacetic acid as a source of carbon. the enzyme appeared to be homogeneous as judged by disc-gel electrophoresis and sedimentation equilibrium measurements. the average molecular weight determined by three independent procedures was 106,000; the protein was globular and was dissociated ... | 1977 | 838683 |
| modified sutter's arginine dihydrolase medium for pseudomonas speciation. | sutter's arginine dihydrolase medium has been modified to obtain maximum yields of arginine dihydrolase. bromothymol blue is the indicator for alkalinity in sutter's medium. by adding glucose and lowering the ph of the medium, more positive reactions were obtained in 24 hr as well as a sharper color contrast to the base medium which facilitated reading the reactions. the modified sutter's medium was included in the routine biochemical schema for speciation of pseudomonads isolated from cosmetic ... | 1977 | 838673 |
| magnitude of the equilibrium isotope effect on carbon-tritium bond synthesis. | the exchange of tritium from 3hoh into the methyl group of pyruvate catalyzed by 6-phospho-2-keto-3-deoxygluconate aldolase (6-phospho-2-keto-3-deoxy-d-gluconate d-glyceraldehyde-3-phosphate-lyase, ec 4.1.2.14) of pseudomonas putida shows an equilibrium isotope effect of 0.78. from this value and the deuterium effect on the fumarase equilibrium (thomson, j.f. (1960) arch. biochem. biophys. 90, 1), one can calculate by use of the relative fractionation factors of hartshorn and shiner (hartshorn, ... | 1977 | 836851 |
| properties of l-methionine gamma-lyase from pseudomonas ovalis. | the distribution of bacterial l-methionine gamma-lyase (l-methionine methanethiollyase (deaminating) (ec 4.4.1.11) was investigated, and pseudomonas ovalis (ifo 3738) was found to have the highest activity of enzyme, which was inducibly formed by addition of l-methionine to the medium. l-methionine gamma-lyase, purified to homogeneity from ps. ovalis, has a molecular weight of about 173 000 and consists of nonidentical subunits (mol wt: 40 000 and 48 000). the enzyme exhibits absorption maxima a ... | 1977 | 831771 |
| isolation of a mutant tol plasmid with increased activity and transmissibility from pseudomonas putida (arvilla) mt-2. | strains with greater ability to dissimilate m-toluate were obtained from the wild-type pseudomonas putida (arvilla) mt-2 that harbors the tol plasmid. increased growth of a mutant strain on aromatic substrates was coupled with simultaneous increase in the activity of metapyrocatechase, an enzyme coded by the tol plasmid, without changing its catalytic properties. in the mutant and the wild-type strains, the inducer specificity and the induction kinetics of metapyrocatechase synthesis were the sa ... | 1977 | 830645 |
| superoxide dismutase from mycobacterium tuberculosis. | 1. a superoxide dismutase [ec 1.15.1.1] was purified about 275-fold with a yield of 34% from mycobacterium tuberculosis, strain h37ra (attenuated strain), grown on a sauton medium for two months. the purified enzyme was homogeneous as judged by polyacrylamide gel electrophoresis, and by analytical ultracentrifugation and sedimentation equilibrium studies. 2. the molecular weight of the enzyme was estimated to be approximately 88,000 by sedimentation equilibrium analysis. since the molecular weig ... | 1976 | 828161 |
| isolation of plasmid deoxyribonucleic acid from pseudomonas putida. | conditions suitable for reproducible recovery of covalently closed circular deoxyribonucleic acid from strains of pseudomonas putida containing degradative plasmids (cam, sal, oct, etc.) have been defined. these degradative plasmids could not be isolated by the usual procedure, whereas rp1, an r factor of the p group, present in the isogenic strain of p. putida, was isolated equally well by either the usual procedure or the modified procedure. characterization by electron microscopy of rp1 deoxy ... | 1976 | 816778 |
| multiple forms of rat liver cytochrome p-450. immunochemical evidence with antibody against cytochrome p-448. | purified hepatic cytochrome p-448 from 3-methylcholanthrene-treated rats was used to produce antibody in rabbits. the cytochrome p-448 antibody (igg fraction) isolated from immune rabbit serum is quite specific and precipitates purified rat liver cytochrome p-448 at low antibody to protein ratios when assayed by the ouchterlony double diffusion technique. purified hepatic cytochrome p-450 from phenobarbital-treated rats cross-reacts poorly with the cytochrome p-448 antibody as do purified rabbit ... | 1976 | 815258 |
| physiological function of the pseudomonas putida ppg6 (pseudomonas oleovorans) alkane hydroxylase: monoterminal oxidation of alkanes and fatty acids. | pseudomonas putida ppg6 is able to utilize purified n-alkanes of six to ten carbon atoms for growth. it can also grow on the primary terminal oxidation products of these alkanes and on 1-dodecanol but not on the corresponding 2-ketones or 1,6-hexanediol, adipic acid, or pimelic acid. revertible point mutants can be isolated which have simultaneously lost the ability to grow on all five n-alkane growth substrates but which can still grow on octanol or nonanol. an acetate-negative mutant defective ... | 1975 | 804473 |
| properties of anthranilate synthetase component ii from pseudomonas putida. | the interaction of pseudomanas putida anthranilate synthetase component ii (as ii) with glutamine, glutamine analogs, and iodoacetamide has been investigated in order to clarify the initial steps in the mechanism for glutamine utilization. as ii is alkylated and irreversibly inactivated by covalent attachment of approximately 1 eg of l-2-amino-4-oxo-5-chloropentanoic acid (chloroketone) or 1 eq of iodoacetamide. alkylation of as ii by chloroketone involves initial formation of an enzyme-inhibito ... | 1976 | 765343 |
| dissociation of the nic plasmid aggregate in pseudomonas putida. | the nic plasmid on conjugal transfer from pseudomonas convexa pc 1 to pseudomonas putida ppg 1 dissociates into an independent fertility factor t and a nontransmissible nic structural gene plasmid. | 1979 | 762028 |
| fertility factors in pseudomonas putida: selection and properties of high-frequency transfer and chromosome donors. | the octane plasmid (oct) in pseudomonas putida strains has been shown to be transferred at low frequency. however, bacteria which had newly received this plasmid showed a transient increase in donor ability. using octane+ p. putida as the donor, the transfer of most chromosomal markers was shown to be independent of oct transfer, whereas the mobilization of the octanoate catabolism genes (octanoic and acetate) was dependent on oct plasmid transfer. the presence of a fertility factor termed fpo h ... | 1979 | 762014 |
| direct hydroxylation in the biosynthesis of hydroxy fatty acid in lipid a of pseudomonas ovalis. | it was found that pseudomonas ovalis iam 1177 had an abundance of hydroxy fatty acids such as 3-hydroxy-decanoic acid, 3-hydroxy-dodecanoic acid and 2-hydroxy-dodecanoic acid in the lipophilic part of the lipopolysaccharide fraction, which comprise 80% of total fatty acids. by using 18o2, it was shown that one oxygen atom from molecular oxygen was incorporated into 2-hydroxy-dodecanoic acid, but not into 3-hydroxy-decanoic acid. the incorporated oxygen atom was specifically located at the hydrox ... | 1979 | 760794 |
| evidence for a transmissible catabolic plasmid in pseudomonas putida encoding the degradation of p-cresol via the protocatechuate ortho cleavage pathway. | 1978 | 751853 | |
| the aromatic alcohol dehydrogenases in pseudomonas putida n.c.i.b. 9869 grown on 3,5-xylenol and p-cresol. | whole cells of pseudomonas putida n.c.i.b 9869, when grown on either 3,5-xylenol or p-cresol, oxidized both m- and p-hydroxybenzyl alcohols. two distinct nad+-dependent m-hydroxybenzyl alcohol dehydrogenases were purified from cells grown on 3,5-xylenol. each is active with a range of aromatic alcohols, including both m- and p-hydroxybenzyl alcohol, but differ in their relative rates with the various substrates. an nad+-dependent alcohol dehydrogenase was also partially purified from p-cresol gr ... | 1978 | 743216 |
| p-cresol and 3,5-xylenol methylhydroxylases in pseudomonas putida n.c.i.b. 9896. | pseudomonas putida n.c.i.b. 9869, when grown on 3,5-xylenol, hydroxylates the methyl groups on 3,5-xylenol and on p-cresol by two different enzymes. 3,5-xylenol methylhydroxylase, studied only in relatively crude extracts, requires nadh, is not active with p-cresol and is inhibited by cyanide, but not by co. the p-cresol methylhydroxylase requires an electron acceptor and will act under anaerobic conditions. it was purified and is a flavocytochrome c of mol.wt. approx. 114,000 consisting of two ... | 1978 | 743215 |
| incorporation of [18o]water in the formation of p-hydroxybenzyl alcohol by the p-cresol methylhydroxylase from pseudomonas putida. | in the hydroxylation of the methyl group of p-cresol by an enzyme from pseudomonas putida the oxygen atom is derived from water. although a second reaction by the same enzyme converts the product, p-hydroxybenzyl alcohol, into the aldehyde, the alcohol is an enzyme-free intermediate. | 1978 | 736904 |
| subunit and amino acid composition of l-arginine deiminase of pseudomonas putida. | 1978 | 729806 | |
| chemical and spectral properties of putidamonooxin, the iron-containing and acid-labile-sulfur-containing monooxygenase of a 4-methoxybenzoate o-demethylase from pseudomonas putida. | gel chromatography indicates that putidamonooxin has a molecular weight of about 126,000. on the other hand, the amino acid composition and the iron-to-protein ratio point to a minimal molecular weight of 33,000 and 31,000 respectively. on sodium dodecylsulfate/polyacrylamide gel electrophoresis the enzyme migrated as a homogeneous band corresponding to a molecular weight of about 40,000. the number of spots found in the tryptic peptide map of the carboxymethylated and digested enzyme indicates ... | 1978 | 729590 |
| circular dichroism and magnetic circular dichroism of iron-sulfur proteins. | circular dichroism (cd) and magnetic circular dichroism (mcd) spectra are reported for the 2-fe ferredoxins from pseudomonas putida and spirulina maxima, chromatium hipip, the 4-fe ferredoxin from bacillus stearothermophilus, and the 8-fe ferredoxin from clostridium pasteurianum. the spectral range spans the near-infrared, visible, and near ultraviolet. in all cases except oxidized 2-fe ferredoxins, electronic absorption is observed continuously from less than 5000 cm-1 to above 30,000 cm-1. the ... | 1978 | 728385 |
| a study of bacteria contaminating refrigerated cooked chicken; their spoilage potential and possible origin. | cooked chicken was allowed to spoil in a normal kitchen refrigerator (variable temperature) and at a standard 4c. after 10 days' storage, bacteria were isolated from the chicken. it was found that the numbers of organisms at variable refrigeration temperature were tenfold higher than those at a uniform 4c. in an attempt to find the sources of contamination, swabs were made of different areas of the kitchen. many of the bacteria isolated from the spoiled chicken, were also isolated from the kitch ... | 1978 | 701783 |
| amine dehydrogenase of pseudomonas putida: properties of the heme-prosthetic group. | there was approximately five times more hemoprotein (amine dehydrogenase) in crude extracts obtained from pseudomonas putida grown on benzylamine than present in extracts from succinate-grown cells. the difference (reduced minus oxidized) spectrum of the purified enzyme possessed alpha,beta, and gamma bands at 550, 523, and 416 nm, respectively. the difference spectrum of the pyridine hemochrome derivative had absorption maxima at 416, 520, and 550 nm. these results, together with the fact that ... | 1978 | 690082 |
| chemical structure and biodegradability of halogenate aromatic compounds. substituent effects on 1,2-dioxygenation of benzoic acid. | dioxygenation of substituted benzoic acids by whole cells of 3-chlorobenzoate-utilizing pseudomonas sp. b 13, benzoate-induced cells of alcaligenes eutrophus b 9 and toluate-grown cells of pseudomonas putida mt-2 was examined. electron-attracting substituents like halogen decreased the reaction rates of benzoate 1,2-dioxygenation. dioxygenation of substituted benzoic acids by p. putida mt-2 was mostly undisturbed by steric effects of the substituents. good correlation resulted between the log vr ... | 1978 | 687664 |
| pseudomonas ovalis ferredoxin: similarity to azotobacter and chromatium ferredoxins. | 1978 | 680138 | |
| the role of enoyl-coa hydratase in the metabolism of isoleucine by pseudomonas putida. | the purpose of the present study was to determine if the enoyl coenzyme a hydratase formed by pseudomonas putida during growth on isoleucine was a unique enzyme specific for isoleucine metabolism. the highest levels of the hydratase were formed during growth on isoleucine intermediates and the lowest levels during growth on glutamate and glucose. data from growth experiments revealed that 2-methyl-3-hydroxybutyryl coenzyme a hydratase, an enzyme unique to isoleucine metabolism and enoyl coenzyme ... | 1978 | 678016 |
| nic, a conjugative nicotine-nicotinate degradative plasmid in pseudomonas convexa. | the plasmid nature of genes specifying degradation of nicotine and nicotinate in pseudomas convexa strain 1 (pc1) is indicated by mitomycin curing and conjugational transfer to other strains. the nic plasmid appears to be compatible with other metabolic plasmids in pseudomonas putida. | 1978 | 670150 |
| magnetic circular dichroism of pseudomonas putida cytochrome p-450 in near infrared region. | magnetic circular dichroism spectra of oxidized, reduced and carbonmonoxy reduced forms of cytochrome p-450 from d-camphor grown pseudomonas putida (p-450cam) were studied in the near infrared region (650 to 1200 nm) at various temperatures in the presence of d-camphor. oxidized p-450cam with camphor exhibited positive (+) and negative (-) magnetic cd bands at 825 and 970 nm, respectively, and both of them were assigned to faraday b terms. the magnetic cd spectrum of reduced p-450cam in the pres ... | 1978 | 667105 |
| transformation of pseudomonas putida with chromosomal dna. | 1978 | 666840 | |
| [naphthalene oxidation by a pseudomonas putida strain carrying a mutant plasmid]. | naphthalene oxidation by a parent and a mutant strain of pseudomonas putida was studied. the parent strain contained a plasmid npl-1 which controlled oxidation of naphthalene to salicylic acid and was capable of oxidizing salicylate. the mutant strain did not oxidize salicylate because of a mutation in salicylate hydroxylase; it contained also a mutant plasmid npl-41 which determined constitutive synthesis of naphthalene oxygenase. salicylic acid which accumulated as a product of naphthalene cat ... | 1978 | 661635 |
| anthranilate synthetase component ii from pseudomonas putida. covalent structure and identification of the cysteine residue involved in catalysis. | the complete amino acid sequence of carboxamidomethylated anthranilate synthetase component ii (as ii) from pseudomonas putida has been determined by analysis of cyanogen bromide fragments, tryptic peptides from the citraconylated protein, and by analysis of subdigests of these peptides. as ii is a single polypeptide chain of 197 residues having a calculated molecular weight of 21,684. previous studies (goto, y., keim, p. s., zalkin, h., and heinrikson, r. l. (1976) j. biol. chem, 251, 941-949) ... | 1978 | 659439 |
| regulation of the degradative pathway enzymes coded for by the tol plasmid (pwwo) from pseudomonas putida mt-2. | pseudomonas putida mt-2 carries a plasmid (tol, pwwo) which codes for a single set of enzymes responsible for the catabolism of toluene and m- and p-xylene to central metabolites by way of benzoate and m- and p-toluate, respectively, and subsequently by a meta cleavage pathway. characterization of strains with mutations in structural genes of this pathway demonstrates that the inducers of the enzymes responsible for further degradation of m-toluate include m-xylene, m-methylbenzyl alcohol, and m ... | 1978 | 659369 |
| mutation to increased resistance to phenol in pseudomonas putida. | 1978 | 656570 | |
| [characteristics of pseudomonas putida plasmid dnas]. | physico-chemical characteristics of plasmid dnas isoalted from pseudomonas putida g7 were studied as well as the behavior of these dnas in th eourse of chromatography on columns with sepharose 4b and kieselguhr with methylated albumin (mac). this strain was found to contain several plasmid dnas having molecular weights of 33-36x10(6), 15-18x10(6), and 3-5x10(6) dalton. the plasmid dnas of biodegradation are supposed to be located in the vicinity of chromosomes, and only a small part of them is c ... | 1978 | 651695 |
| phosphonate utilization by bacteria. | bacteria able to use at least one of 13 ionic alkylphosphonates of o-alkyl or o,o-dialkyl alkylphosphonates as phosphorus sources were isolated from sewage and soil. four of these isolates used 2-aminoethylphosphonic acid (aep) as a sole carbon, nitrogen, and phosphorus source. none of the other phosphonates served as a carbon source for the organisms. one isolate, identified as pseudomonas putida, grew with aep as its sole carbon, nitrogen, and phosphorus source and released nearly all of the o ... | 1978 | 618850 |
| changes in cytochrome content and electron transport patterns in pseudomonas putida as a function of growth phase. | optical absorbance difference spectra of membrane vesicles prepared from aerobically grown pseudomonas putida indicated that, when harvested in logarithmic phase, the cells contained one c-type cytochrome and two or three b-type cytochromes, one of which was cytochrome o. as the cells grew into stationary phase and the oxygen concentration of the medium dropped to essentially zero, an additional component believed to be cytochrome d was produced. both the o- and d-type cytochromes might function ... | 1978 | 618838 |
| microbiological transformations of terpenes: part xxiv--pathways of degradation of linalool, geraniol, nerol & limonene by pseudomonas incognita (linalool strain). | 1977 | 615106 | |
| isolation of mutants with altered metabolic control of the nah plasmid-encoded catechol meta-cleavage pathway. | two types of mutants which displayed altered regulation of the nah catabolic plasmid-encoded catechol meta-cleavage pathway were isolated in pseudomonas putida. altered metabolic control was indicated by assay of catechol 2,3-dioxygenase. in one type of mutant the catechol 2,3-dioxygenase was synthesized constitutively. in the other type the range of carbon sources which induce the catechol 2,3-dioxygenase was increased. | 1977 | 614009 |
| metabolism of d- and l-lactate by pseudomonas putida. | pseudomonas putida grew at the same rate with the same molar growth yield on d-, l, or dl-lactate as the sole source of carbon for growth. d- and l- lactate were utilized simultaneously and at the same rate when the organism was grown on dl-lactate (ratio of d isomer to l isomer of 1:1). growth on either isomer alone, or in combination, caused the induction of both a d-lactate, and an l-lactate dehydrogenase. both enzymes were particulate and used dichlorophenolindophenol, or oxygen, but not na ... | 1977 | 614007 |
| microbiological transformations of terpenes: part xxiii--fermentation of geraniol, nerol & limonene by a soil pseudomonad, pseudomonas incognita (linalool strain). | 1977 | 612543 | |
| in vitro inhibition of human peripheral blood lymphocyte transformation by an extract of pseudomonas putida. | an extract prepared from a psychrophilic strain of pseudomonas putida was found to cause a dose-dependent inhibition of [h3]tdr incorporation into human peripheral blood lymphocytes stimulated with pha, cona, pwm, or in a mixed lymphocyte reaction. the inhibition was found not to be the result of cytotoxicity, culture medium depletion of a component necessary for lymphocyte transformation, or interference with label uptake by blast lymphocytes. the extract was most effective when added prior to ... | 1977 | 608686 |
| a comparative study of the nah and tol catabolic plasmids in pseudomonas putida. | a comparative study of the nah and tol catabolic plasmids was carried out to provide information for future genetic manipulation experiments involving these two plasmids. the plasmids were studied in a strain of p. putida and its mutant derivatives. the nah and tol plasmids were found to be incompatible. under the conditions used in these experiments the tol plasmid transferred into some strains into which nah was unable to transfer. the use of mutants to remove certain catabolic activities enco ... | 1977 | 603460 |
| alpha-pinene metabolism by pseudomonas putida. | by using metabolically altered mutants and acrylate, novel putative intermediates of alpha-pinene metabolism by pseudomonas putida pin11 were detected. they were characterized as 3-isopropylbut-3-enoic acid and (zeta)-2-methyl-5-isopropylhexa-2,5-dienoic acid. | 1977 | 597274 |
| microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products. | microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ... | 1977 | 596877 |
| metabolism of dibenzothiophene by a beijerinckia species. | beijerinckia b8/36 when grown with succinate in the presence of dibenzothiophene, accumulated (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene and dibenzothiophene-5-oxide in the culture medium. each metabolite was isolated in crystalline form and characterized by a variety of chemical techniques, cis-naphthalene dihydrodiol dehydrogenase, isolated from pseudomonas putida, oxidized (+)-cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene to a compound that was tentatively identified as 1,2-dihydroxydi ... | 1977 | 596875 |
| the purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from pseudomonas putida. | the enzyme that catalyses the hydroxylation of the methyl group of p-cresol was purified from pseudomonas putida. it has mol.wt. 115000 and appears to contain two subunits of equal molecular weight. one subunit is a c-type cytochrome and the other is a flavoprotein. reduction of the cytochrome occurred on addition of substrate. the same enzyme catalyses both p-cresol hydroxylation and the further oxidation of the product, 4-hydroxybenzyl alcohol. the stoicheiometry of acceptor reduced per molecu ... | 1977 | 588247 |
| transposition of a beta-lactamase locus from rp1 into pseudomonas putida degradative plasmids. | the beta-lactamase gene from the rp1 plasmid transposes into at least two pseudomonas putida degradative plasmids. donor strains that carry rp1 (bla+ tet+ apha+) and a degradative plasmid yield transconjugants that have only the bla+ marker of rp1. this occurs in up to 80% of all bla+ transconjugants. segregation of the bla+ marker requires the presence of a degradative plasmid in the donor and is only observed in transconjugants that have received degradative markers. the bla+ tet apha transcon ... | 1977 | 584205 |
| stereospecific hydrogen loss in the conversion of [2h7]isobutyrate to beta-hydroxyisobutyrate in pseudomonas putida. the stereochemistry of beta-hydroxyisobutyrate dehydrogenase. | 1979 | 573276 | |
| stereochemistry of the conversion of methacrylate to beta-hydroxyisobutyrate in pseudomonas putida. | 1979 | 572832 | |
| formaldehyde dehydrogenase from pseudomonas putida. purification and some properties. | formaldehyde dehydrogenase was isolated and purified in an overall yield of 12% from cell-free extract of pseudomonas putida c-83 by chromatographies on columns of deae-cellulose, deae-sephadex a-50, and hydroxyapatite. the purified enzyme was homogeneous as judged by disc gel electrophoresis and was most active at ph 7.8 using formaldehyde as a substrate. the enzyme was also active toward acetaldehyde, propionaldehyde, glyoxal, and pyruvaldehyde, though the reaction rates were low. the enzyme w ... | 1979 | 571868 |
| [interrelationships between carnitine metabolism and fatty acid assimilation in pseudomonas putida (author's transl)]. | the carnitine metabolism and some relations to the fatty acid metabolism were studied in pseudomonas putida by means of control of growth, analysis of metabolites, and determination of enzyme activites. the strain grew on gamma-butyrobetaine, d,l- and l-carnitine, glycinebetaine, choline, d,l-norcarnitine, d,l-gamma-amino-beta-hydroxybutyrate, and d,l-beta-hydroxybuty-rate. although the strain used straight-chain fatty acids of 2-16 c-atoms, it was only able to grow on o-acyl-l-carnitines of 10 ... | 1978 | 565193 |
| the metabolism of caffeine by a pseudomonas putida strain. | 1) a bacterium capable of growing aerobically with caffeine (1,3,7-trimethylxanthine) as sole source of carbon and nitrogen was isolated from soil. the morphological and physiological characteristics of the bacterium were examined. the organism was identified as a strain of pseudomonas putida and is referred to as pseudomonas putida c1. 15 additional caffeine-degrading bacteria were isolated, and all of them were also identified as pseudomonas putida strains. the properties of the isolates are d ... | 1977 | 561017 |
| cometabolism of products of 1,1,1-trichloro-2,2-bis (p-chlorophenyl)ethane (ddt) by pseudomonas putida. | 1977 | 560402 | |
| [purification and crystallization of a superoxide dismutase from pseudomonas putida]. | 1979 | 551636 | |
| coexistence of different pathways in the metabolism of n-propylbenzene by pseudomonas sp. | pseudomonas desmolytica s449b1 and pseudomonas convexa s107b1 grown on n-propylbenzene oxidized n-propylbenzene to beta-phenylpropionic acid and benzoic acid by initial oxidation of the n-propyl side chain and the following beta-oxidation, respectively. the same strains also oxidized n-propylbenzene to 3-n-propylcatechol by initial oxidation of positions 2 and 3 of the aromatic nucleus. a ring fission product, 2-hydroxy-6-oxononanoic acid, was also isolated from the culture broth. together with ... | 1979 | 543699 |
| regulation of membrane peptides by the pseudomonas plasmid alk regulon. | pseudomonas putida strains carrying the plasmid alk genes will grow on n-alkanes. induced alk+ strains contain membrane activities for alkane hydroxylation and dehydrogenation of aliphatic primary alcohols. p. putida cytoplasmic and outer membranes can be separated by sucrose gradient centrifugation after disruption of cells by either mild detergent lysis or passage through a french press. both the membrane component of alkane hydroxylase and membrane alcohol dehydrogenase fractionated with the ... | 1979 | 533768 |
| local anesthetics block induction of the pseudomonas alk regulon. | the local anesthetics procaine and piperocaine blocked induction of the plasmid-determined enzymatic activities involved in the metabolism of n-alkanes in pseudomonas putida. procaine reversibly inhibited existing alkane hydroxylase activity. induction of a soluble aliphatic amidase activity was not affected. these results support the hypothesis that induction of the plasmid-determined alkane metabolic system in p. putida involves a membrane component(s). | 1979 | 533765 |